EP0000645B1 - Isopenicillins, processes for their preparation, and compositions containing them - Google Patents

Isopenicillins, processes for their preparation, and compositions containing them Download PDF

Info

Publication number
EP0000645B1
EP0000645B1 EP78300169A EP78300169A EP0000645B1 EP 0000645 B1 EP0000645 B1 EP 0000645B1 EP 78300169 A EP78300169 A EP 78300169A EP 78300169 A EP78300169 A EP 78300169A EP 0000645 B1 EP0000645 B1 EP 0000645B1
Authority
EP
European Patent Office
Prior art keywords
compound
oxo
mmol
heptane
thia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP78300169A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP0000645A1 (en
Inventor
Ralph Floyd Hall
William Francis Huffman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GlaxoSmithKline LLC
SmithKline Beecham Corp
Original Assignee
SmithKline Corp
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Corp, SmithKline Beecham Corp filed Critical SmithKline Corp
Publication of EP0000645A1 publication Critical patent/EP0000645A1/en
Application granted granted Critical
Publication of EP0000645B1 publication Critical patent/EP0000645B1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D513/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
    • C07D513/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains two hetero rings
    • C07D513/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D205/00—Heterocyclic compounds containing four-membered rings with one nitrogen atom as the only ring hetero atom
    • C07D205/02—Heterocyclic compounds containing four-membered rings with one nitrogen atom as the only ring hetero atom not condensed with other rings
    • C07D205/06—Heterocyclic compounds containing four-membered rings with one nitrogen atom as the only ring hetero atom not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • C07D205/08—Heterocyclic compounds containing four-membered rings with one nitrogen atom as the only ring hetero atom not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with one oxygen atom directly attached in position 2, e.g. beta-lactams
    • C07D205/085—Heterocyclic compounds containing four-membered rings with one nitrogen atom as the only ring hetero atom not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with one oxygen atom directly attached in position 2, e.g. beta-lactams with a nitrogen atom directly attached in position 3
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D499/00—Heterocyclic compounds containing 4-thia-1-azabicyclo [3.2.0] heptane ring systems, i.e. compounds containing a ring system of the formula:, e.g. penicillins, penems; Such ring systems being further condensed, e.g. 2,3-condensed with an oxygen-, nitrogen- or sulfur-containing hetero ring

Definitions

  • This invention relates to isopenicillins showing antibacterial activity, to processes for their preparation, and to pharmaceutical compositions containing them.
  • the acyl group is preferably an acyl group known to impart antibacterial activity as a substituent in the 7- or 6-positions of cephalosporins or _penicillins respectively.
  • acyl refers to acyl groups represented by the general formulae where
  • the 5- or 6-membered heterocyclic rings referred to above include thienyl, furyl, thiazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, triazolyl, tetrazolyl, pyridyl, and pyrimidyl.
  • Each heterocyclic group may be unsubstituted or substituted with one or two substituents selected from lower alkyl, halo, hydroxy, nitro, lower alkoxy, aryl such as phenyl, or lower aralkyl.
  • the terms lower alkyl or lower alkoxy refer to groups containing one to six carbon atoms.
  • acyl groups include the following examples:
  • the isopenicillin compounds of this invention decompose rapidly when the 2-carboxylic acid group is present in the free acid form. However, the compounds are stable when the acid is present as a salt or is protected with a protective ester group. Therefore, it is apparent to the skilled chemist that all chemical reactions performed on these compounds must be done under conditions which take this fact into account.
  • a carboxylic acid protective ester residue refers to those ester groups which are commonly employed to block or protect the carboxylic acid functionality while reactions are carried out on other functional groups within the molecule.
  • the term has acquired a definite meaning within the f3- lactam and organic chemical arts and many useful groups within this term are known in the art. These protective groups are known for the ease with which they may be cleaved to regenerate the carboxylic acid group.
  • the term refers to those groups known in the art which can be cleaved by mild basic hydrolysis and/or hydrogenation in basic solution.
  • ester protecting groups include lower alkyl such as methyl, 2,2,2-trichloroethyl, f3-iodoethyl, C1-Ca-alkanoylmethyl, N-phthalimidomethyl, benzoylmethyl, halobenzoylmethyl, methylbenzoylmethyl, methanesulfonylbenzoylmethyl, phenylbenzoylmethyl, benzyl, p-nitrobenzyl, p-methoxybenzyl, and benzhydryl.
  • the choice of which ester group to use is well within the ability of one skilled in the art. Factors which are considered include what subsequent reaction conditions the group must withstand and what conditions for removing the protecting ester are desirable. Particularly preferred esters are methyl, benzyl and benzhydryl.
  • the selection of the proper protecting group is not critical to our invention since the point of novelty of our invention lies within the new isopenicillin nucleus and not within the ester groups substituted thereon
  • carboxyl protecting groups are not intended to be exhaustive. A person skilled in the art knows the purpose of these groups and is able to properly choose from the groups known and described in the art. Many articles and books have described the subject of protecting reactive groups, for example J. F. W. McOmie, "Protective Groups in Organic Chemistry", Plenum Press, 1973.
  • salts of carboxylic acids for pharmaceutical formulations. These salts have improved properties, such as solubility, over the free acids.
  • useful cations include alkali metals such as sodium and potassium, alkaline earth metals and ammonium cations from inorganic or organic amine bases. These salts are prepared when the protective ester groups are hydrolyzed by base or when the isopenicillin nucleus is formed by base treatment as described below.
  • salts of other acid moieties present within the acyl group of the compounds are prepared in the same manner as described above.
  • the compounds of this invention may exist in hydrate or solvate form.
  • the amount of water or solvent may vary.
  • the compounds of this invention where R is acylamino and M'is hydrogen or a pharmaceutically acceptable cation have antibacterial activity against Gram-positive and Gram-negative organisms.
  • Minimum inhibitory concentrations (MIC's) against a variety of bacteria are shown in Table 1 for representative compounds. Data for standard antibacterial agents, penicillin V and 2-thienylmethyl- penicillin are included.
  • the active compounds or their salts can be dissolved in water and used to sterilize laboratory equipment or for the treatment or prevention of bacterial infections in warm-blooded mammals such as man.
  • R is acylamino and M is a carboxylic acid protecting ester group also exhibit antibacterial activity, for example against B. subtilis. These compounds may be used in the same manner as described for the compounds where M is not an ester group.
  • R is amino or azido and/or M is a carboxylic acid protecting ester group are useful as intermediates for the preparation of the therapeutically active compounds.
  • R is azido
  • reduction by chemical or catalytic methods also gives the useful free amino derivative.
  • halogen or halo shall mean fluorine, chlorine, bromine or iodine.
  • the compounds of this invention are novel bicyclic A-lactams which are prepared by a totally synthetic route.
  • the key starting materials are cis-3-azido-4-oxo-2-azetidinylmethyl iodide (1 a) and cis-3-t-butoxycarbonylamino-2-hydroxymethyl-4-oxoazetidine ⁇ 1b ⁇ .
  • These compounds can be prepared in good yield via a ketene-imine cyclization reaction of azidoacetic acid and methyl N-(2,4-dimethoxybenzyl)iminoacetate and subsequent chemical modification, all as set forth in Belgian Patent No. 841,234.
  • Schemes 1, 2 and 3 set forth different reactions which may be used to prepare compounds of this invention. It is readily apparent to one skilled in the art that the reactions set forth in these Schemes may be carried out by various methods in various sequences. In particular, at various points along the reaction pathway set forth in each of the Schemes, the R substituent may be converted from azido to amino and the amino group subsequently acylated with a desired acyl group. The most advantageous times to perform these conversions would be readily apparent to a person skilled in the art.
  • the reaction sequence set forth in Scheme 1 involves first, a condensation of the A-lactam 1 with an ester of glyoxylic acid to give the ⁇ -hydroxy- ⁇ -azetidinyl acetic acid derivative (2).
  • the hydroxy group of this compound is converted to a halo derivative, such as chloro by the reaction with thionyl chloride, and the halo derivative is reacted with a salt of thiolacetic acid to give the sulfur-containing compound (3).
  • Cyclization of compound (3) to the desired isopenicillin derivative can be effected by treatment with a base such as cyclohexylamine. If R is azido, reduction to the amino derivative followed by acylation with the desired acyl group gives the compounds of this invention. If M is a protecting ester group, it may be removed by base hydrolysis to give the compounds where M is a cation.
  • a preferred route to the antibacteriaf compounds of this invention involves treating compound 3 where R is acylamino and M is a cation such as sodium with a base such as cyclohexylamine.
  • R is t-butoxycarbonylamino
  • M is benzhydryl
  • thionyl chloride followed by potassium thiolacetate gives compound 3 (R and M are as above).
  • any organic primary and secondary amine which preferentially hydrolyzes the thiolacetate moiety over attacking the A-lactam moiety gives the desired product.
  • the selectivity of action is a result of choosing a base with the proper balance between basicity and nucleophilicity. The selection of the proper base is within the ability of a person skilled in the art.
  • the preferred route is run in an organic solvent, preferably an aprotic solvent.
  • the reaction is run at a temperature and a period of time which maximizes the formation of product and minimizes product decomposition. Temperatures may range from -30 to 30° with about 0° being a preferred temperature.
  • Scheme 2 sets forth a different reaction sequence for converting the ⁇ -hydroxy compound 2 into the isopenicillins.
  • the hydroxy group is converted into a chloro group as outlined above in Scheme 1.
  • the resulting chloro derivative is treated with sodium triphenylmethylmercaptide to give derivative 4.
  • Cyclization of derivative 4 can be effected by treatment with metal ions such as silver or mercury or by treatment with a strong acid such as trifluoroacetic acid.
  • the a-chloro compound (5) may also be converted directly into the desired isopenicillin as outlined in Scheme 3.
  • Reagents useful for this conversion include hydrogen sulfide, sodium hydrosulfide, sodium sulfide and tetramethylguanidinium hydrosulfide.
  • Acylations of the compounds of this invention are effected by standard methods.
  • the carboxylic acid group which will be the carbonyl group in the acyl moiety is activated by known methods including mixed anhydride, activated esters, and acid halides.
  • use of coupling reagents such as dicyclohexylcarbodiimide and carbonyldiimidazole is a possible method of acylation.
  • any sensitive group in the acyl moiety for example, hydroxyl or carboxyl, can be protected by a standard protecting group such as those described previously and/or known in the art.
  • acyl groups which are particularly useful in this invention contain an asymmetric carbon atom. It is understood that each optical isomer separately and as mixtures of the isomers are within the scope of this invention. It has been found that the D-isomer is particularly useful and therefore is a preferred isomer as with the mandelamido containing compounds.
  • the cis-fused isopenicillin ring system may exist as d and I isomers.
  • the carboxylic acid group at position 2 can be in the a or f3 configuration and results in an additional center of asymmetry. All possible stereoisomers are within the scope of this invention.
  • Benzyl glyoxylate (1.97 g, 12 mmol) was dissolved in toluene (25 ml) and a small amount was distilled out to dry the solution. The solution was cooled to 90° and the product of Preparation 1 (1 g, 3.97 mmol) was added. The reaction was heated for 5.5 hours under argon at 90°. The solution was evaporated in vacuo and the residue was chromatographed on silica gel (100 g). The product was eluted with 10% ethyl acetate in benzene, 1.28 g (78%).
  • the reaction mixture was extracted with ethyl acetate and the product isolated in the usual way to give crude material (16 g) which was dissolved in dichloromethane and allowed to crystallize overnight at -23°C. The solution was filtered and the crystals dried to give a white crystalline solid; 2.6 g, mp 159-160.5°. The mother liquors were combined and chromatographed on silica gel to afford additional semicrystalline product (3.71 g). The crystalline material was a single diastereoisomer while the material isolated by chromatography was a mixture of diastereoisomers.
  • the combined extracts are washed copiously with water and brine.
  • the dried extracts are distilled in vacuo to give 4.5 g of clear orange gum which was rapidly chromatographed on a column of 90 g of silica gel. with methylene chloride and 20% ethyl acetate in methylene chloride as eluants to give the condensation products, 1.66 g (64%).
  • Example 2 The compound from Example 2 (65 mg, 0.171 mmol) was hydrogenated at atmospheric pressure in ethyl acetate (2 ml) in the presence of Pt0 2 (130 mg) for three hours. The mixture was filtered and evaporated to give the title product, 60 mg.
  • Example 1 The compound of Example 1 (35 mg, 0.115 mmol) was hydrogenated in ethyl acetate with Pto 2 as catalyst (70 mg) in the same manner as in Example 3 to give the title product, 32 mg.
  • the compound of Preparation 12 (731 mg, 0.935 mmol) was dissolved in dichloromethane (30 ml) and anisole (4 ml), cooled to 0° under argon and treated with trifluoroacetic acid (36 ml). The mixture was stirred at 0° for 20 minutes and then was added rapidly to a cold mixture of aqueous NaHCO a layered with ethyl acetate. The layers were separated and the aqueous layer was re-extracted with ethyl acetate. The combined organic phases were washed with brine, dried and evaporated to give the product which was chromatographed on silica gel (100 g).
  • the title compound was also prepared by dissolving the intermediate chloro compound prepared above in anhydrous dimethylformamide (1.5 ml) and cooling to -20 0 .-The cold solution was treated with a sodium sulfide-dimethylformamide solution (0.7 ml) which was prepared as follows:

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
EP78300169A 1977-07-26 1978-07-20 Isopenicillins, processes for their preparation, and compositions containing them Expired EP0000645B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US819197 1977-07-26
US05/819,197 US4122086A (en) 1977-07-26 1977-07-26 Isopenicillins

Publications (2)

Publication Number Publication Date
EP0000645A1 EP0000645A1 (en) 1979-02-07
EP0000645B1 true EP0000645B1 (en) 1981-01-28

Family

ID=25227463

Family Applications (1)

Application Number Title Priority Date Filing Date
EP78300169A Expired EP0000645B1 (en) 1977-07-26 1978-07-20 Isopenicillins, processes for their preparation, and compositions containing them

Country Status (7)

Country Link
US (1) US4122086A (enrdf_load_stackoverflow)
EP (1) EP0000645B1 (enrdf_load_stackoverflow)
JP (1) JPS5424891A (enrdf_load_stackoverflow)
DE (1) DE2860370D1 (enrdf_load_stackoverflow)
DK (1) DK328578A (enrdf_load_stackoverflow)
IE (1) IE47037B1 (enrdf_load_stackoverflow)
IT (1) IT1097306B (enrdf_load_stackoverflow)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257947A (en) * 1977-08-03 1981-03-24 Smithkline Corporation 3-Amino-2-hydroxy, halo or mercaptomethyl-4-oxoazetidines
US4174316A (en) * 1978-08-14 1979-11-13 Merck & Co., Inc. 4-Iodomethylazetidin-2-one
ATE6065T1 (de) * 1978-08-14 1984-02-15 Merck & Co. Inc. Verfahren zur herstellung von thienamycin und zwischenprodukten.
US4290947A (en) * 1979-04-27 1981-09-22 Merck & Co., Inc. Process for the preparation of thienamycin and intermediates
JPS62248766A (ja) * 1986-04-21 1987-10-29 タキゲン製造株式会社 扉用ハンドル装置
JPS63140464U (enrdf_load_stackoverflow) * 1987-03-09 1988-09-16
US5563264A (en) * 1993-02-10 1996-10-08 Shionogi & Co., Ltd. Preparation of βlactam compounds
DE69427100T2 (de) * 1993-02-10 2001-11-22 Shionogi & Co., Ltd. Herstellung von Beta-lactamverbindungen und Zwischenprodukte
US5496816A (en) * 1994-03-14 1996-03-05 Merck & Co., Inc. Carbapenem antibacterial compounds, compositions containing such compounds and methods of treatment

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK194776A (da) * 1975-05-05 1976-11-06 Smithkline Corp Antibakterielle midler og mellemprodukter dertil

Also Published As

Publication number Publication date
IT1097306B (it) 1985-08-31
IE47037B1 (en) 1983-11-30
IT7826043A0 (it) 1978-07-24
JPS6143357B2 (enrdf_load_stackoverflow) 1986-09-26
JPS5424891A (en) 1979-02-24
EP0000645A1 (en) 1979-02-07
DE2860370D1 (en) 1981-03-19
US4122086A (en) 1978-10-24
DK328578A (da) 1979-01-27
IE781493L (en) 1979-01-26

Similar Documents

Publication Publication Date Title
CA1059988A (en) Process for the manufacture of enol derivatives
EP0000645B1 (en) Isopenicillins, processes for their preparation, and compositions containing them
EP0178980B1 (fr) Dérivés de céphalosporines, procédé d'obtention et leur application à titre d'antibiotiques
US4155912A (en) 2-Methylpenem-3-carboxylic acid antibiotics
US4670431A (en) Beta-lactam antibacterial agents
EP0003115A1 (en) Penicillins, per se and for use as antibacterially active antibiotics and their production
CH628900A5 (fr) Procede de preparation de thio-oximes derivees de cephalosporines et de penicillines.
US4200572A (en) Substituted azetidinones
IE43845B1 (en) 4-thia-1-azabicyclo/4.2.0/oct-2-ene derivatives
US4071529A (en) Derivatives of 6-aminopenicillanic acid
US4035359A (en) 6α, β-Substituted penicillin derivatives
US4590073A (en) 6-substituted penicillanic acid 1,1-dioxide compounds
US3842072A (en) Certain 2alpha-(2-hydroxymethyl-2-propylmercapto)-3-amino-azetidin-4-one compounds
US4145418A (en) Thienopyridine substituted cephalosporins
US4103086A (en) 8-Oxo-4-thia-1-azabicyclo (4.2.0)-oct-2-ene derivatives
US4000154A (en) 3-Substituted-6β-(amino- and acylamino)-7-oxo-1,3-diazabicyclo[3.2.0]-h
CA1120935A (en) MONOCYCLIC .beta.-LACTAMS WITH ANTIBACTERIAL ACTIVITY
JPH0780840B2 (ja) ４，４‐ジアルキル‐２‐アゼチジノン類の製造法
EP0122002B1 (en) Process for preparing azetidinone derivatives
CA1102308A (en) Carbon and oxygen analogs of cephalosporins
JP2689412B2 (ja) 保護基の除去方法
EP0066373B1 (en) Beta-lactam derivatives, a process for their preparation and compositions containing them
US4613462A (en) 6-substituted penicillanic acid 1,1-dioxide compounds
EP0093564B1 (en) Beta-lactam antibiotics
US3705160A (en) Thiazacyclic hydroxy compounds and process for their manufacture

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Designated state(s): BE CH DE FR GB LU NL SE

17P Request for examination filed
GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Designated state(s): BE CH DE FR GB LU NL SE

REF Corresponds to:

Ref document number: 2860370

Country of ref document: DE

Date of ref document: 19810319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 19810731

REG Reference to a national code

Ref country code: CH

Ref legal event code: PFA

Free format text: SMITHKLINE BECKMAN CORPORATION

REG Reference to a national code

Ref country code: FR

Ref legal event code: CD

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: LU

Payment date: 19830707

Year of fee payment: 6

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 19840619

Year of fee payment: 7

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 19840813

Year of fee payment: 7

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: CH

Payment date: 19840903

Year of fee payment: 7

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: SE

Payment date: 19840930

Year of fee payment: 7

Ref country code: BE

Payment date: 19840930

Year of fee payment: 7

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: NL

Payment date: 19860731

Year of fee payment: 9

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Effective date: 19870721

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CH

Effective date: 19870731

BERE Be: lapsed

Owner name: SMITHKLINE BECKMAN CORP.

Effective date: 19870731

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Effective date: 19880201

NLV4 Nl: lapsed or anulled due to non-payment of the annual fee
PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 19880331

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DE

Effective date: 19880401

GBPC Gb: european patent ceased through non-payment of renewal fee
REG Reference to a national code

Ref country code: FR

Ref legal event code: ST

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 19881117

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Effective date: 19890731

EUG Se: european patent has lapsed

Ref document number: 78300169.6

Effective date: 19880713

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT