EA201992615A1 - METHODS FOR ISOLATING CELLS WITHOUT USING TRANSGENIC MARKER SEQUENCES - Google Patents

METHODS FOR ISOLATING CELLS WITHOUT USING TRANSGENIC MARKER SEQUENCES

Info

Publication number
EA201992615A1
EA201992615A1 EA201992615A EA201992615A EA201992615A1 EA 201992615 A1 EA201992615 A1 EA 201992615A1 EA 201992615 A EA201992615 A EA 201992615A EA 201992615 A EA201992615 A EA 201992615A EA 201992615 A1 EA201992615 A1 EA 201992615A1
Authority
EA
Eurasian Patent Office
Prior art keywords
methods
marker sequences
isolating cells
transgenic
transgenic marker
Prior art date
Application number
EA201992615A
Other languages
Russian (ru)
Inventor
Саиксиа Гао
Руи Жанг
Джинхинг Лиу
Аарон Хуммел
Зарир Вагхчхипавала
Матиас Лабс
Original Assignee
Институте Оф Генетисс Анд Девелопментал Биологй, Чинесе Асадемй Оф Ссиенсес
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Институте Оф Генетисс Анд Девелопментал Биологй, Чинесе Асадемй Оф Ссиенсес filed Critical Институте Оф Генетисс Анд Девелопментал Биологй, Чинесе Асадемй Оф Ссиенсес
Publication of EA201992615A1 publication Critical patent/EA201992615A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/10—Vectors comprising a non-peptidic targeting moiety

Abstract

Настоящее изобретение относится к способам целенаправленного редактирования в растении, растительной клетке или материале, которое скомбинировано с параллельным введением фенотипически селектируемого признака. Кроме того, предлагаются способы, не содержащие этап введения трансгенной селективной маркерной последовательности.The present invention relates to methods for targeted editing in a plant, plant cell or material that is combined with the parallel introduction of a phenotypically selectable trait. In addition, methods are provided that do not include the step of introducing a transgenic selective marker sequence.

EA201992615A 2017-09-01 2018-05-07 METHODS FOR ISOLATING CELLS WITHOUT USING TRANSGENIC MARKER SEQUENCES EA201992615A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201710778196 2017-09-01
PCT/CN2018/085829 WO2018202199A1 (en) 2017-05-05 2018-05-07 Methods for isolating cells without the use of transgenic marker sequences

Publications (1)

Publication Number Publication Date
EA201992615A1 true EA201992615A1 (en) 2020-03-10

Family

ID=64015902

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201992615A EA201992615A1 (en) 2017-09-01 2018-05-07 METHODS FOR ISOLATING CELLS WITHOUT USING TRANSGENIC MARKER SEQUENCES

Country Status (3)

Country Link
CN (1) CN108795972B (en)
EA (1) EA201992615A1 (en)
WO (1) WO2018202199A1 (en)

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CN109666693B (en) * 2018-12-29 2022-08-16 北京市农林科学院 Application of MG132 in editing receptor genome by base editing system
CN110066797A (en) * 2019-01-23 2019-07-30 福建上源生物科学技术有限公司 Editor's label ben-1 sgRNA target site and its CRISPR/Cas9 are total to editing system and application altogether
CN110628795B (en) * 2019-09-30 2021-07-16 北京市农林科学院 Cell enrichment technology using inactivated screening agent resistance gene as report system for A.G base substitution and application thereof
CN110669775B (en) * 2019-09-30 2021-07-16 北京市农林科学院 Application of differential proxy technology in enrichment of A.G base substitution cells
CN110564752B (en) * 2019-09-30 2021-07-16 北京市农林科学院 Application of differential agent technology in enrichment of C.T base substitution cells
CN110669794B (en) * 2019-09-30 2021-07-16 北京市农林科学院 Cell enrichment technology of C.T base substitution by using mutant screening agent resistance gene as report system and application thereof
CN110628794B (en) * 2019-09-30 2021-07-16 北京市农林科学院 Cell enrichment technology of C.T base substitution by taking inactivated screening agent resistance gene as report system and application thereof
CN112725348B (en) * 2019-10-28 2022-04-01 安徽省农业科学院水稻研究所 Gene and method for improving single-base editing efficiency of rice and application of gene
MX2022005328A (en) 2019-11-05 2022-07-21 Pairwise Plants Services Inc Compositions and methods for rna-encoded dna-replacement of alleles.
AU2020378387A1 (en) * 2019-11-07 2022-01-20 Qingdao Kingagroot Chemical Compound Co., Ltd. Method for generating new mutations in organisms, and application thereof
WO2021123397A1 (en) * 2019-12-20 2021-06-24 Biogemma IMPROVING EFFICIENCY OF BASE EDITING USING TypeV CRISPR ENZYMES
CN111549053B (en) * 2020-05-22 2022-06-07 北京市农林科学院 Method for single nucleotide mutation of cauliflower
CN111961126B (en) * 2020-08-31 2022-04-08 中国农业科学院作物科学研究所 Application of TaVQ25 gene in regulation and control of resistance of wheat to powdery mildew and banded sclerotial blight
CN112322655B (en) * 2020-10-22 2023-06-30 肇庆华夏凯奇生物技术有限公司 Base editing system free from restriction of gene sequence, and preparation method and application thereof
CN113178229B (en) * 2021-05-31 2022-03-08 吉林大学 Deep learning-based RNA and protein binding site recognition method
GB202109586D0 (en) 2021-07-02 2021-08-18 Tropic Biosciences Uk Ltd Method for editing banana genes
CN114045302A (en) * 2021-11-12 2022-02-15 三亚中国农业科学院国家南繁研究院 Single-base editing vector and construction and application thereof
WO2023111130A1 (en) * 2021-12-17 2023-06-22 Tropic Biosciences UK Limited Modified agrobacteria for editing plants
WO2023245113A1 (en) * 2022-06-16 2023-12-21 Intellia Therapeutics, Inc. Methods and compositions for genetically modifying a cell

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Also Published As

Publication number Publication date
CN108795972A (en) 2018-11-13
CN108795972B (en) 2023-07-14
WO2018202199A1 (en) 2018-11-08

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