EA201790944A1 - METHOD OF OBTAINING VIRUS-LIKE PARTICLES OF HUMAN PAPILLOMAVIRUS - Google Patents

METHOD OF OBTAINING VIRUS-LIKE PARTICLES OF HUMAN PAPILLOMAVIRUS

Info

Publication number
EA201790944A1
EA201790944A1 EA201790944A EA201790944A EA201790944A1 EA 201790944 A1 EA201790944 A1 EA 201790944A1 EA 201790944 A EA201790944 A EA 201790944A EA 201790944 A EA201790944 A EA 201790944A EA 201790944 A1 EA201790944 A1 EA 201790944A1
Authority
EA
Eurasian Patent Office
Prior art keywords
particles
human papillomavirus
obtaining virus
protein
hpv
Prior art date
Application number
EA201790944A
Other languages
Russian (ru)
Inventor
Ян Джэ Чо
Кван Сун Ким
Original Assignee
Айджин, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Айджин, Инк. filed Critical Айджин, Инк.
Publication of EA201790944A1 publication Critical patent/EA201790944A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Настоящее изобретение предусматривает способ получения вирусоподобных частиц (VLP) на основе белка L1 папилломавируса человека (HPV). Настоящее изобретение обеспечивает получение белка L1 HPV с высокой степенью чистоты с использованием одного хроматографического процесса способом, в котором загрязнения вначале удаляют посредством осаждения сульфатом аммония, а затем дополнительно удаляют из раствора, содержащего соль в концентрации от 0,35 М до 0,60 М, посредством диализа.The present invention provides a method for producing virus-like particles (VLPs) based on human papillomavirus (HPV) L1 protein. The present invention provides high purity HPV L1 protein using a single chromatographic process in a manner in which contaminants are first removed by precipitation with ammonium sulfate and then additionally removed from a solution containing salt in a concentration of 0.35 M to 0.60 M through dialysis.

EA201790944A 2014-12-26 2015-10-12 METHOD OF OBTAINING VIRUS-LIKE PARTICLES OF HUMAN PAPILLOMAVIRUS EA201790944A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20140190902 2014-12-26
PCT/KR2015/010740 WO2016104923A1 (en) 2014-12-26 2015-10-12 Method for preparing virus-like particles of human papillomavirus

Publications (1)

Publication Number Publication Date
EA201790944A1 true EA201790944A1 (en) 2017-11-30

Family

ID=56150907

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201790944A EA201790944A1 (en) 2014-12-26 2015-10-12 METHOD OF OBTAINING VIRUS-LIKE PARTICLES OF HUMAN PAPILLOMAVIRUS

Country Status (4)

Country Link
KR (1) KR101825996B1 (en)
EA (1) EA201790944A1 (en)
RU (1) RU2677336C2 (en)
WO (1) WO2016104923A1 (en)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL183781B1 (en) * 1994-05-16 2002-07-31 Merck & Co Inc Vaccines against papilloma virus
US5821087A (en) * 1995-03-30 1998-10-13 Merck & Co., Inc. Production of recombinant human papillomavirus type II protein utilizing papillomavirus 6/11 hybrid DNA
ES2572628T3 (en) * 1997-09-05 2016-06-01 Medimmune, Inc. In vitro method for disassembly / reassembly of particles similar to human papillomavirus (VLP)
EP1608767B1 (en) * 2003-03-24 2012-02-22 Merck Sharp & Dohme Corp. Optimized expression of hpv 31 l1 in yeast
BRPI0811016B1 (en) * 2007-04-29 2021-09-21 Xiamen Innovax Biotech Co., Ltd. LI TRUNCATE PROTEIN FROM HUMAN PAPILOMA VIRUS TYPE 16
KR100959145B1 (en) 2008-03-21 2010-05-25 중앙대학교 산학협력단 Process for Producing and Purifying Human Papillomavirus Virus-Like Particles
TR201802597T4 (en) * 2009-06-19 2018-03-21 Eyegene Inc Vaccine for cervical cancer.
KR101182663B1 (en) * 2010-06-29 2012-09-18 주식회사 유라코퍼레이션 Process box in one body nametag
KR101559622B1 (en) * 2012-07-30 2015-10-13 중앙대학교 산학협력단 An Efficient Method for Purifying Virus-Like Particles of Human Papillomavirus

Also Published As

Publication number Publication date
WO2016104923A1 (en) 2016-06-30
RU2015149330A (en) 2018-11-30
RU2015149330A3 (en) 2018-11-30
KR20160080060A (en) 2016-07-07
KR101825996B1 (en) 2018-02-09
RU2677336C2 (en) 2019-01-16

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