EA201692516A1 - Применение селектируемого маркерного гена в способе и системе трансформации протопластов сахарной свеклы - Google Patents

Применение селектируемого маркерного гена в способе и системе трансформации протопластов сахарной свеклы

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Publication number
EA201692516A1
EA201692516A1 EA201692516A EA201692516A EA201692516A1 EA 201692516 A1 EA201692516 A1 EA 201692516A1 EA 201692516 A EA201692516 A EA 201692516A EA 201692516 A EA201692516 A EA 201692516A EA 201692516 A1 EA201692516 A1 EA 201692516A1
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Eurasian Patent Office
Prior art keywords
sequence
protoplasts
sugar beet
selective marker
application
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EA201692516A
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English (en)
Inventor
Ги Вейенс
Марк Лефебвр
Шивеговда Тамманнаговда
Мюриэль Ломмель
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Сесвандерхаве Н.В.
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Application filed by Сесвандерхаве Н.В. filed Critical Сесвандерхаве Н.В.
Publication of EA201692516A1 publication Critical patent/EA201692516A1/ru

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    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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Abstract

Настоящее изобретение относится к способу трансформации протопластов сахарной свеклы, включающему следующие стадии: получение протопластов из замыкающих клеток устьица, выделенных из растения сахарной свеклы, трансформация протопластов конструкцией нуклеиновой кислоты, содержащей интересующую нуклеотидную последовательность и последовательность селективного маркера, применение к in vitro культуре указанных протопластов и регенерирование растений сахарной свеклы из выживших протопластов, имеющих интегрированную конструкцию нуклеиновой кислоты, содержащую интересующую последовательность и последовательность селективного маркера, где последовательность селективного маркера представляет собой мутированную последовательность BvALS113, несущую в своей последовательности мутацию в 113 аминокислотном положении аланина в тирозин.
EA201692516A 2014-06-12 2015-06-12 Применение селектируемого маркерного гена в способе и системе трансформации протопластов сахарной свеклы EA201692516A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP2014062288 2014-06-12
PCT/EP2015/063144 WO2015189376A1 (en) 2014-06-12 2015-06-12 Use of a selectable marker gene in sugar beet protoplasts transformation method and system

Publications (1)

Publication Number Publication Date
EA201692516A1 true EA201692516A1 (ru) 2017-04-28

Family

ID=53434320

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Application Number Title Priority Date Filing Date
EA201692516A EA201692516A1 (ru) 2014-06-12 2015-06-12 Применение селектируемого маркерного гена в способе и системе трансформации протопластов сахарной свеклы

Country Status (5)

Country Link
US (1) US20170335336A1 (ru)
EP (1) EP3155000A1 (ru)
CN (1) CN106459988A (ru)
EA (1) EA201692516A1 (ru)
WO (1) WO2015189376A1 (ru)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9321183D0 (en) * 1993-10-14 1993-12-01 Zeneca Ltd A method of plant transformation
EA021541B9 (ru) * 2007-04-04 2017-04-28 Басф Плант Сайенс Гмбх Рекомбинантный или мутантный полинуклеотид для придания растениям толерантности к гербицидам, ингибирующим ацетогидроксикислотную синтазу (ahas) (варианты), растение, не встречающееся в природе, и его семена, способ борьбы с сорняками, способ получения трансгенного растения, толерантного к соединениям, ингибирующим ahas, способ идентификации или отбора клетки растения, ткани растения или растения или части растения, способ предотвращения нежелательного вегетативного роста и способ идентификации растения
UA126466C2 (uk) * 2010-10-15 2022-10-12 Баєр Інтеллекчуел Проперті Гмбх Толерантний до інгібуючого als гербіциду мутант буряка звичайного
UA117821C2 (uk) * 2012-12-13 2018-10-10 Сесвандерхаве Н.В. Спосіб одержання мутантної рослини цукрового буряку, що стійка до гербіцидів інгібіторів als

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Publication number Publication date
EP3155000A1 (en) 2017-04-19
US20170335336A1 (en) 2017-11-23
CN106459988A (zh) 2017-02-22
WO2015189376A1 (en) 2015-12-17

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