EA201400713A1 - Генеральная стратегия для скрининга библиотеки антител - Google Patents

Генеральная стратегия для скрининга библиотеки антител

Info

Publication number
EA201400713A1
EA201400713A1 EA201400713A EA201400713A EA201400713A1 EA 201400713 A1 EA201400713 A1 EA 201400713A1 EA 201400713 A EA201400713 A EA 201400713A EA 201400713 A EA201400713 A EA 201400713A EA 201400713 A1 EA201400713 A1 EA 201400713A1
Authority
EA
Eurasian Patent Office
Prior art keywords
general strategy
screening antibodies
selective
antibodies libraries
libraries
Prior art date
Application number
EA201400713A
Other languages
English (en)
Inventor
Штефан Беккер
Тим Хайзелер
Александер Маасс
Харальд Кольмар
Original Assignee
Мерк Патент Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Мерк Патент Гмбх filed Critical Мерк Патент Гмбх
Publication of EA201400713A1 publication Critical patent/EA201400713A1/ru

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/08—Methods of screening libraries by measuring catalytic activity

Abstract

Раскрывается общеприменимый способ для селективного ковалентного присоединения репортерной молекулы к реплицируемой единице, который позволяет получать специфически связывающие агенты из одного цикла скрининга библиотеки. Например, селективное биотинилирование фаговых частиц и дрожжевых клеток, демонстрирующих связывающий агент к любой заданной мишени, может быть достигнуто путем применения сочетанной ферментативной реакции, которая включает пероксидазу, оксидазу и каталазу.
EA201400713A 2011-12-16 2012-12-14 Генеральная стратегия для скрининга библиотеки антител EA201400713A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11009901.7A EP2605014A1 (en) 2011-12-16 2011-12-16 General strategy for antibody library screening
PCT/EP2012/005162 WO2013087215A1 (en) 2011-12-16 2012-12-14 General strategy for antibody library screening

Publications (1)

Publication Number Publication Date
EA201400713A1 true EA201400713A1 (ru) 2014-12-30

Family

ID=47428549

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201400713A EA201400713A1 (ru) 2011-12-16 2012-12-14 Генеральная стратегия для скрининга библиотеки антител

Country Status (14)

Country Link
US (1) US9733240B2 (ru)
EP (2) EP2605014A1 (ru)
JP (1) JP2015505675A (ru)
KR (1) KR20140104493A (ru)
CN (1) CN103998934A (ru)
AU (1) AU2012351104B2 (ru)
BR (1) BR112014014427A2 (ru)
CA (1) CA2859247A1 (ru)
EA (1) EA201400713A1 (ru)
ES (1) ES2742303T3 (ru)
IL (1) IL233133A (ru)
MX (1) MX2014007094A (ru)
SG (1) SG11201403073PA (ru)
WO (1) WO2013087215A1 (ru)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113999772A (zh) * 2021-09-29 2022-02-01 佛山奥素博新科技有限公司 一种基于数字微流控系统的酵母表面展示方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9712818D0 (en) * 1996-07-08 1997-08-20 Cambridge Antibody Tech Labelling and selection of specific binding molecules
EP1634074B1 (en) * 2003-06-12 2009-08-12 Perkinelmer Las, Inc. Hydrolytic substrates for an analyte-dependent enzyme activation system
JP2008228637A (ja) * 2007-03-20 2008-10-02 Tokushima Bunri Univ 蛍光相関分光測定法を用いた過酸化水素量の測定方法及びその利用方法
NZ582618A (en) * 2007-07-13 2012-07-27 Prometheus Lab Inc Drug selection for lung cancer therapy using antibody-based arrays
EP2268317B1 (en) * 2008-03-14 2020-02-26 VisEn Medical, Inc. Integrin targeting agents and in vivo and in vitro imaging methods using the same
AU2010248884B2 (en) * 2009-05-14 2015-04-02 Nestec S.A. Biomarkers for determining sensitivity of breast cancer cells to HER2-targeted therapy

Also Published As

Publication number Publication date
US9733240B2 (en) 2017-08-15
EP2605014A1 (en) 2013-06-19
WO2013087215A1 (en) 2013-06-20
AU2012351104B2 (en) 2018-08-09
CN103998934A (zh) 2014-08-20
SG11201403073PA (en) 2014-07-30
JP2015505675A (ja) 2015-02-26
US20140357514A1 (en) 2014-12-04
KR20140104493A (ko) 2014-08-28
IL233133A0 (en) 2014-07-31
EP2791677A1 (en) 2014-10-22
IL233133A (en) 2017-12-31
CA2859247A1 (en) 2013-06-20
MX2014007094A (es) 2014-07-22
EP2791677B1 (en) 2019-05-15
ES2742303T3 (es) 2020-02-13
AU2012351104A1 (en) 2014-07-31
BR112014014427A2 (pt) 2017-06-13

Similar Documents

Publication Publication Date Title
MX2023003418A (es) Moleculas multiespecificas de union a antigenos y usos de estas.
MX2022012083A (es) Moleculas de union de antigeno multiespecificas y usos de las mismas.
WO2013011011A3 (en) Method of reversibly staining a target cell
WO2012129242A3 (en) Isolation of polymerase-nucleic acid complexes and loading onto substrates
WO2012106718A3 (en) Methods of using gene expression signatures to select a method of treatment, predict prognosis, survival, and/or predict response to treatment
CA2832534C (en) Method for the generation of compact tale-nucleases and uses thereof
WO2010091182A3 (en) Methods for screening candidate agents for modulating prorenin and renin, assays for detecting prorenin, and antibodies used therein
MX355060B (es) Molecula multiespecifica de union a antigeno que tiene funcion alternativa a la funcion del factor viii de coagulacion sanguinea.
CR20130669A (es) Molèculas biespecìficas de uniòn a antìgeno activadoras de cèlulas t
EA201790309A1 (ru) Антитела к fap, способы их получения и применения
BRPI0812878C1 (pt) anticorpo isolado ou parte funcional do mesmo, vetor, método para produzir um anticorpo ou uma parte funcional do mesmo, para produzir uma célula produtora de anticorpo e para produzir um anticorpo capaz de se ligar especificamente a rsv, composição, e, uso de um anticorpo ou parte funcional do mesmo ou de uma composição ou de vetor
MY195714A (en) Antigen-Binding Molecule Capable of Binding to Two or More Antigen Molecules Repeatedly
EA201692242A1 (ru) Композиции с высокой концентрацией антител
EA201491833A1 (ru) Основанные на клетках перекрестные анализы и их применение
EA201690465A1 (ru) Способ оценки эффектов композиции, содержащей микроорганизмы, на кишечную микробиоту
WO2012154794A3 (en) Enzyme concentration and assays
EA201391389A1 (ru) Кристаллы антител против склеростина и составы на их основе
WO2013059582A3 (en) Small molecule inhibitors of histone deacteylases
EP2141497B8 (en) Method for the analysis of circulating antibodies
MX2022006578A (es) Anticuerpos especificos para cd47, pd-l1 y sus usos.
EA201490450A1 (ru) Новые ингибиторы ферментов
MX349801B (es) Sustratos de enzimas de ácido nucleico.
GB2542576A (en) Method and kit of detecting the absence of micro-oranisms
MX2014014566A (es) Metodo para detectar un aglutinante multiespecifico.
WO2014210416A8 (en) Helicase suppression of non-template amplification