EA200900513A1 - ПОЛУЧЕНИЕ β-ЛАКТАМОВЫХ АНТИБИОТИКОВ - Google Patents

ПОЛУЧЕНИЕ β-ЛАКТАМОВЫХ АНТИБИОТИКОВ

Info

Publication number
EA200900513A1
EA200900513A1 EA200900513A EA200900513A EA200900513A1 EA 200900513 A1 EA200900513 A1 EA 200900513A1 EA 200900513 A EA200900513 A EA 200900513A EA 200900513 A EA200900513 A EA 200900513A EA 200900513 A1 EA200900513 A1 EA 200900513A1
Authority
EA
Eurasian Patent Office
Prior art keywords
tripeptide
module
specific
hpgcv
pgcv
Prior art date
Application number
EA200900513A
Other languages
English (en)
Other versions
EA016155B1 (ru
Inventor
Маркус Ханс
Рулоф Ари Ланс Бовенберг
Пол Классен
Ремон Бур
ВАН ДЕР Ян Метске ЛААН
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA200900513A1 publication Critical patent/EA200900513A1/ru
Publication of EA016155B1 publication Critical patent/EA016155B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oncology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Настоящее изобретение описывает способ получения N-α-аминогидроксифенилацетил или N-α-аминофенилацетил β-лактамового антибиотика, включающий IPNS-катализируемое превращение предшественника трипептида гидроксифенилглицилцистеинилвалин (HpgCV) или фенилглицилцистеинил-валин (PgCV), соответственно, в N-гидроксифенилглицил или N-фенилглицил β-лактамовый антибиотик, соответственно. Трипептид HpgCV или трипептид PgCV затем может быть получен контактированием аминокислот гидроксифенилглицина (Hpg) или фенилглицина (Pg), цистеина (С) и валина (V) с нерибосомальной пептидной синтетазой (NRPS) для того, чтобы осуществить образование трипептида HpgCV или трипептида PgCV, NRPS, содержащей первый модуль M1, специфичный к Hpg или Pg, второй модуль М2, специфичный к С, и третий модуль М3, специфичный к V. Более того, IPNS обеспечивает обладание улучшенной активностью в этом превращении, так же как и катализирование образования трипептидов. Также представлена клетка-хозяин, способная к ферментативному производству β-лактамовых антибиотиков с N-α-аминогидроксифенилацетил или N-α-аминофенилацетил боковыми цепями.
EA200900513A 2006-10-05 2007-10-02 ПОЛУЧЕНИЕ β-ЛАКТАМОВЫХ АНТИБИОТИКОВ EA016155B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06121817 2006-10-05
EP07113095 2007-07-25
PCT/EP2007/060460 WO2008040731A2 (en) 2006-10-05 2007-10-02 Production of beta-lactam antibiotics

Publications (2)

Publication Number Publication Date
EA200900513A1 true EA200900513A1 (ru) 2009-08-28
EA016155B1 EA016155B1 (ru) 2012-02-28

Family

ID=38787063

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200900513A EA016155B1 (ru) 2006-10-05 2007-10-02 ПОЛУЧЕНИЕ β-ЛАКТАМОВЫХ АНТИБИОТИКОВ

Country Status (9)

Country Link
US (1) US8293511B2 (ru)
EP (1) EP2084270B1 (ru)
KR (1) KR101444483B1 (ru)
BR (1) BRPI0719263A2 (ru)
EA (1) EA016155B1 (ru)
ES (1) ES2447491T3 (ru)
MX (1) MX2009003594A (ru)
SI (1) SI2084270T1 (ru)
WO (1) WO2008040731A2 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006007293B4 (de) * 2006-01-31 2023-04-06 OSRAM Opto Semiconductors Gesellschaft mit beschränkter Haftung Verfahren zum Herstellen eines Quasi-Substratwafers und ein unter Verwendung eines solchen Quasi-Substratwafers hergestellter Halbleiterkörper
HUE035540T2 (en) 2010-06-22 2018-05-02 Dsm Sinochem Pharm Nl Bv Air bubble fermentation process
CN104093831A (zh) 2012-01-31 2014-10-08 中化帝斯曼制药有限公司荷兰公司 半合成抗生素生产中的类MbtH蛋白

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9621486D0 (en) * 1996-10-15 1996-12-04 Isis Innovation Oxygenase enzymes & method
DE19909146A1 (de) * 1999-03-03 2000-09-28 Mohamed A Marahiel Verfahren zur gezielten biologischen Synthese von Peptiden
EP1326983B1 (en) * 2000-10-13 2005-04-06 Ecopia Biosciences Inc. Gene cluster for ramoplanin biosynthesis
MX2007006780A (es) * 2004-12-10 2007-08-06 Dsm Ip Assets Bv Produccion de beta-lactamas en celulas individuales.

Also Published As

Publication number Publication date
ES2447491T3 (es) 2014-03-12
EP2084270B1 (en) 2013-11-20
KR101444483B1 (ko) 2014-10-02
KR20090086981A (ko) 2009-08-14
EP2084270A2 (en) 2009-08-05
WO2008040731A2 (en) 2008-04-10
SI2084270T1 (sl) 2014-03-31
BRPI0719263A2 (pt) 2014-07-15
WO2008040731A3 (en) 2008-05-22
MX2009003594A (es) 2009-04-23
US20100009404A1 (en) 2010-01-14
EA016155B1 (ru) 2012-02-28
US8293511B2 (en) 2012-10-23

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Legal Events

Date Code Title Description
PC4A Registration of transfer of a eurasian patent by assignment
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

TC4A Change in name of a patent proprietor in a eurasian patent