EA043767B1 - MOBILE BOX FOR MICROBIOLOGICAL SAFETY - Google Patents

Description

The invention relates to the field of medical equipment and is intended for use in equipping individual workplaces of employees working with pathogenic biological agents (PBA) in the field, and provides protection for the operator and the environment from contamination by aerosols arising when working with PBA.

Currently, microbiological safety cabinets are an integral part of research related to the study of pathogens of dangerous infectious diseases. Microbiological safety cabinets are designed to protect the operator, laboratory space and the environment from exposure to aerosols and splashes that may occur when working with pathogenic biological agents, as well as to protect experimental samples from external factors. Boxes are equipped with individual workplaces of employees working with pathogenic biological agents, in accordance with the requirements for ensuring biological safety and protecting personnel from infection.

Traditional microbiological safety boxes are large in size and have significant weight (more than 200 kg), which hinders their transportation and use in the field. This indicates a clear need to develop a mobile, lightweight, collapsible and quickly assembled microbiological safety cabinet for field teams.

From sources of scientific and technical information, a portable biological safety glove box is known (patent CN 204431285, IPC B25J 21/02), made of transparent thermoplastic polyurethane (TPU) in the form of a rectangular parallelepiped and having a system for creating negative pressure at one end of the body, at the other end filtration device. In each of the two sides of the body there are two working holes in which disposable medical gloves are located, on the upper side there is a hole for supplying liquid or gas inside, and there are also support rods and a locking device for fixing the support rod. However, this biosafety glovebox is designed for benchtop testing, which is not always feasible in the field. Also, this box does not have a device for transferring additional equipment during research, and the lack of lighting limits research in the dark.

The objective of the invention is to create a mobile, lightweight, quickly disassembled and easy to transport by all types of transport microbiological safety box for carrying out work by mobile teams in the field with pathogenic biological agents (PBA) of pathogenicity group IIV and meeting the requirements of regulatory documents for laboratory equipment when working with PBA.

The technical result consists in the implementation of the specified purpose.

The technical result is achieved by the fact that the mobile microbiological safety box has an insulating working chamber made of PVC 0.5 mm thick by high-temperature splicing with additional sealing of the seams, a transfer gateway with glove ports, an inflatable rigid base made of AirDeck, an external filter ventilation unit (FVU) with control panel for all box functions and indicator lamp; Insulated frame arches made of low-density polyethylene (HDPE) are installed on the sides of the working chamber and the transfer gate; on the side surface of the working chamber there is a panel with more than 1 sealed input for connecting the FVU and internal filters; more than 1 railing washer is installed on the surface of the box for attaching longitudinal struts and more than 1 longitudinal strut to impart rigidity to the structure in the longitudinal direction; The front part of the working chamber of the box is made of transparent PVC film, into which glove ports are built-in.

In addition, the box has more than 1 tension strap to secure the box to the desktop;

in addition, the box has more than 1 sealed zipper;

in addition, the box is equipped with an ultraviolet irradiation lamp (UVR);

in addition, the box is equipped with a socket block with more than 1 connector for connecting additional electrical equipment in the box;

In addition, the box is equipped with more than 1 LED lamp.

The inventive mobile microbiological safety box is an insulating means of protection in the form of a ventilated chamber with a transfer gateway made of polymer materials. Reliability and tightness of the seams are ensured by the use of high-temperature fusion technology, as well as high-frequency currents. To maintain the shape and overall dimensions in cross section, the frame of the inventive box is made in the form of arcs made of low-density polyethylene (HDPE). In the longitudinal direction, the shape and overall dimensions of the box are supported by carbon fiber struts, which are installed in rail washers. A transfer gateway with glove ports allows you to safely place the necessary equipment and tools in the work area without turning off the filter ventilation unit (FVU).

The filter-ventilation unit ensures maintenance of the set pressure minus 50 Pa and the volume of supply and removal of air necessary for ventilation of the working chamber. The box control system automatically supports the specified operating parameters. The supplied and exhaust air undergoes two-stage cleaning through anti-aerosol HEPA filters H14.

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The inventive mobile microbiological safety box can be used in field conditions without the use of special countertops and support devices. With the help of tension belts, the inventive mobile microbiological safety box is securely fixed on the work table; an inflatable rigid base made of AirDeck creates a flat and solid work surface. This ensures that work is completed without the risk of overturning the test samples, laboratory glassware and equipment located in the work area.

The front part of the working chamber is made of transparent polymer material and has an arched shape, which allows for improved visual control over the work being carried out in the working area of the proposed box, since the operator’s visual axis runs perpendicular to the surface of the polyurethane film. For comfort and greater mobility of the operator when performing manipulations in the work area, glove ports are built into the front part of the working chamber of the inventive box, to which nitrile gloves are connected, which are impenetrable and resistant to aggressive environments. The gloves are attached to the sleeves of the glove ports of the working chamber and the transfer sluice through a plastic cuff using O-rings.

The zippers of the transfer airlock and the working chamber are sealed gas-proof zippers with plastic teeth. Sealed zippers are designed in such a way that they exclude the possibility of simultaneous unfastening on one side and the appearance of unprotected through access to the inside of the box.

An ultraviolet irradiation lamp (UVR) allows for disinfection of the working area of the box before placing the sample in the box chamber and after performing work with PBA.

The socket block allows you to connect the necessary additional electrical equipment in the working chamber of the box.

LED lamps mounted on frame arches allow you to carry out work in low light conditions, as well as in the dark.

The control panel located on the filter ventilation unit (FVU) is used to control all possible functions of the box: turning on and off the FVU, UV lamp, lighting, setting the timer for the UV lamp, selecting the language for displaying information, and managing the information menu. The information display allows you to read information about the state of the system (pressure differential level, battery charge, temperature, humidity) and quickly respond based on the received data. Using an indicator lamp on the FVU, the operator evaluates the pressure values in the box chamber. When the system is operating normally with pressure readings within the required limits, the lamp lights up green. When the pressure goes beyond the set limits, an audible alarm sounds and the indicator lamp flashes red. If the seal of the box is broken or if the filters are clogged, the alarm system is triggered: a sound signal is generated and the message ATTENTION is displayed on the control panel display! VIOLATION OF REGIME!. The indicator lamp flashes red. Threshold values of parameters, when exceeded, the alarm system is activated, are: minus 20 - minus 90 Pa.

The claimed invention is illustrated by drawings.

In fig. Figure 1 shows a general view of the proposed mobile microbiological safety box, where 1 is the working chamber of the box; 2 - transfer gateway; 3 - tension belts; 4 - inflatable rigid base made of Airdeck; 5 - frame arcs; 6 - rail washers; 7 - longitudinal struts; 8 - socket block; 9 - glove ports of the working chamber; 10 - gateway glove ports; 11 - sealed zippers; 12 internal filters; 13 - external filters; 14 - air ducts; 15 - filter-ventilation unit; 16 - control panel; 17 - information display; 18 - indicator lamp; 19 - panel with terminals for connecting the FVU; 20 - ultraviolet irradiation lamp (UVR); 21 - LED lamps.

In fig. Figure 2 shows a diagram of the control panel of the filter-ventilation unit (FVU), where 22 is an indicator lamp, 23 is an information display, 24 is a pressure sensor.

In fig. 3 - distribution of zones of the proposed box.

In fig. 4 shows a diagram of connecting gloves to the sleeves of glove ports, where 25 is a glove; 26 - glove sealing rings; 27 - plastic cuff; 28 - sealing ring of the sleeve; 29 - sleeve; 30 - sleeve edging; 31 - edging of the glove.

In fig. 5 - the claimed box in assembled condition.

The box is stored folded in a transport bag.

The inventive box is prepared for operation as follows.

Open the transport bag and remove the insulating chamber and the FVU from it. Straighten the chamber on the work surface, unzipping the sealed zippers to let air in. Then the base of the box is inflated using a compressor. Install longitudinal struts into rail washers. Using tension straps, secure the box to the work surface. The tension force of the belts is achieved by adjusting them along the length. Next, connect the FVU to the box. Completely unfasten the outer and inner sealed zippers and screw in the internal filters. The UV lamp is placed in the upper part of the working chamber in the holding loops, secured with clamps and

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Claims (3)

connect to the appropriate connector inside the working chamber of the box. Install gloves in the glove ports of the working chamber and the transfer gateway. The working chamber of the box is disinfected using a UV lamp. Then all the necessary equipment and tools are placed in the working chamber of the box. Devices operating from a 220 V network are connected to the socket block. Close the internal sealed zipper of the transfer gateway, then fasten the outer sealed zipper of the transfer gateway. The FVU is turned on, after which the FVU automatically creates a pressure difference in the working chamber relative to the ambient pressure minus 50±10 Pa, the indicator lamp lights up green. Working negative pressure is maintained automatically. If it is necessary to place additional equipment in the working chamber of the box during operation (with the FVU turned on), you must make sure that the internal sealed zipper is zipped all the way, and only after that the external sealed zipper is unfastened. Place all the necessary additional equipment into the transfer lock and fasten the external sealed zipper. Then open the internal sealed zipper using the glove port of the airlock and move the equipment into the work chamber, after which the internal sealed zipper is closed. After completion of work, disinfection is carried out using ultraviolet radiation. Turn off the power to the FVU, unfasten the outer and inner sealed zippers of the transfer gateway. Then the surfaces and contents of the working chamber and transfer gate are sanitized using a liquid disinfectant. After disinfection, equipment and tools used in the work are removed from the working chamber and transfer gate. Disconnect the UV lamp from the power connector, loosen the clamps holding the lamp and remove it from the working chamber. Unscrew the internal and external filters and immerse them in a container filled with a 6% hydrogen peroxide solution with the addition of 0.5% detergent. The filter holding time is at least 2 hours. After disinfection, the filters are disposed of. Disconnect the FVU from the box. Fasten the internal and external sealed zippers so that 10-15 cm remain unfastened. Remove the longitudinal struts from the rail washers. Unfasten the tension straps and remove air from the base. By moving the side parts of the box longitudinally towards each other, assemble the box and place it in a transport bag. The design features and technical characteristics of the proposed mobile lightweight microbiological safety box provide the necessary conditions for working with pathogenic pathogens of groups I-IV and comply with the requirements of regulatory documents for laboratory equipment when working with pathogenic pathogens. The inventive box has low weight and small dimensions, is convenient for transportation by all types of transport, is easy to use and is intended for the work of mobile teams in the field, where prompt deployment and compliance with biological safety measures is a prerequisite. The applicant has produced a prototype of a mobile lightweight microbiological safety cabinet, which has passed tests that confirmed its compliance with the requirements of regulatory documents for laboratory equipment when working with pathogenic biological agents. CLAIM 1. Mobile microbiological safety box containing an insulating working chamber with glove ports, characterized in that it is equipped with an inflatable rigid base made of AirDeck, a frame, drawstrings for fixing the box on the desktop and an external filter and ventilation unit (FVU) with a box control panel and an indicator lamp, while the insulating working chamber is made of PVC 0.5 mm thick, obtained using high-temperature splicing technology, and is equipped with a transfer gateway made of polymer material, made with glove ports, and a panel located on the side surface with sealed inputs for connecting the FVU, frame is made in the form of insulated frame arches made of low-pressure polyethylene HDPE located on the sides of the box, equipped with LED lamps, and longitudinal struts to give the box rigidity in the longitudinal direction, installed in rail washers, and the insulating working chamber and the transfer gateway are made with sealed zippers to ensure when they are unfastened, access to the mentioned transfer gateway and working chamber. 2. The box according to claim 1 differs in that it is equipped with an ultraviolet irradiation lamp. 3. The box according to claim 1 differs in that it is equipped with a block of sockets with connectors for connecting additional electrical equipment in the working chamber. -