EA042144B1 - COMPOSITIONS, METHODS AND USES FOR CLEANING, DISINFECTING AND/OR STERILIZING - Google Patents

Description

This application claims priority and filing date benefit under 35 U.S.C. § 119(e) based on U.S. Provisional Application No. 62/526175, filed June 28, 2017, and U.S. Provisional Application No. 62/577225, filed October 26, 2017, the contents of each of which are incorporated into this application in its entirety by reference.

In the United States, approximately 46.5 million surgical procedures and even more invasive medical procedures are performed each year, including approximately 5 million gastrointestinal endoscopies. Each procedure involves bringing the medical device into contact with sterile tissue or mucous membranes of the patient. The main risk of all such procedures is the introduction of pathogens that can lead to infection. Failure to properly clean, disinfect, and/or sterilize a medical device may expose the patient to body fluids and/or tissue contamination from a previous patient, which may result in human-to-human or environmental transmission of pathogens and infect large numbers of people.

Endoscopes are used to diagnose and treat numerous diseases and are part of a family of valuable diagnostic and therapeutic tools. Unfortunately, endoscopes cause more healthcare-associated disease outbreaks than any other medical device. This is mainly due to the fact that endoscopes: 1) are subject to high levels of microbial contamination (bioburden) during use due to the types of body cavities in which said device is used; and 2) fundamentally difficult to clean, disinfect and/or sterilize. For example, in 2012, a source of transmission of carbapenem-resistant enterobacteria (CRE) superbug infections was found in contaminated endoscopes in hospital patients in Seattle, Pittsburgh, and Chicago.

Sterilization of a medical device is preferred because the process destroys or eliminates all forms of microbial life. However, the high temperature and pressure used in sterilization processes are not suitable for heat sensitive medical devices. Such heat-sensitive medical devices should be cleaned using a disinfection process. However, one of the problems with current disinfection processes is that the type and amount of disinfectant agents used are damaging to the medical device. This shortens the overall life of the medical device, which ultimately leads to an increase in medical costs due to the need to replace the medical device with a new one earlier. In addition, given the recent infectious outbreaks caused by contaminated medical devices, a more efficient disinfection method than currently available methods is needed.

The present invention describes disinfectant compositions, methods and uses that provide superior cleaning and disinfection of a medical device, including a temperature-sensitive medical device and a device classified as critical, semi-critical or non-critical. At the same time, the disinfectant compositions, methods, and uses described are less aggressive than conventional compositions, methods, and uses, resulting in a longer service life of the medical device.

Brief description of the invention

In aspects according to the present invention, a composition is described containing hypochlorous acid or free active chlorine and one or more disinfecting agents. The disinfecting agent described in this application may be one or more compounds containing a guanide fragment or a functional group, one or more aldehyde-containing compounds, or one or more organic peroxides, or any combination thereof. The described compound containing a guanide moiety includes one or more compounds containing a biguanide moiety, one or more compounds containing a biguanide moiety, one or more compounds containing a triguanide moiety, or any combination thereof. The described aldehyde-containing compound includes one or more aldehydes, one or more dialdehydes, or any combination thereof.

In other aspects, the present invention describes a composition comprising hypochlorous acid or free active chlorine, one or more disinfecting agents, and one or more surfactants. The disinfecting agent described in this application may be one or more compounds containing a guanide fragment or a functional group, one or more aldehyde-containing compounds, or one or more organic peroxides, or any combination thereof. The described compound containing a guanide moiety includes one or more compounds containing a biguanide moiety, one or more compounds containing a biguanide moiety, one or more compounds containing a triguanide moiety, or any combination thereof. The described aldehyde-containing compound includes one or more aldehydes, one or more dialdehydes, or any combination thereof. The surfactant described in this application may be one or more ionic surfactants,

- 1 042144 one or more zwitterionic (amphoteric) surfactants or one or more nonionic surfactants.

In other aspects according to the present invention describes a composition containing hypochlorous acid or free active chlorine and one or more surfactants. The disinfecting agent described in this application may be one or more compounds containing a guanide fragment or a functional group, one or more aldehyde-containing compounds, or one or more organic peroxides, or any combination thereof. The described compound containing a guanide moiety includes one or more compounds containing a biguanide moiety, one or more compounds containing a biguanide moiety, one or more compounds containing a triguanide moiety, or any combination thereof. The described aldehyde-containing compound includes one or more aldehydes, one or more dialdehydes, or any combination thereof. The surfactant described in this application may be one or more ionic surfactants, one or more zwitterionic (amphoteric) surfactants, or one or more nonionic surfactants.

In other aspects, the present invention describes a composition comprising one or more compounds containing a triguanide moiety and one or more alcohols. The described compound containing a guanide moiety includes one or more compounds containing a biguanide moiety, one or more compounds containing a biguanide moiety, one or more compounds containing a triguanide moiety, or any combination thereof. Described alcohol includes ethanol, methanol, isopropyl alcohol, or any combination thereof.

In other aspects, according to the present invention, a method for cleaning, disinfecting and/or sterilizing a device is described. The described method includes applying the composition described in this application to the device, while applying the composition cleans, disinfects and/or sterilizes the device. The described method may further include rinsing the cleaned, disinfected and/or sterilized device with the rinsing solution described in this application. In other aspects, according to the present invention, a composition described in this application is described for use in cleaning, disinfecting and/or sterilizing a device. In other aspects, according to the present invention, the use of the described composition for cleaning, disinfecting and/or sterilizing a device is described.

In other aspects, the present invention describes a method for cleaning, disinfecting and/or sterilizing a surface area. The described method includes applying the composition described in the invention to the surface area, while applying the composition cleans, disinfects and/or sterilizes the surface area. The described method may further include rinsing the cleaned, disinfected and/or sterilized surface area with a rinsing solution as described herein. In other aspects, according to the present invention, a composition described in this application is described for use in cleaning, disinfecting and/or sterilizing a surface area. In other aspects, according to the present invention, the use of the described composition for cleaning, disinfecting and/or sterilizing a surface area is described.

In other aspects, the present invention describes a method for cleaning, disinfecting and/or sterilizing a microbial infection in a patient. The described method includes the application of the composition described in this application to the patient, while the use of the composition cleans, disinfects and/or sterilizes the microbial infection. In other aspects, according to the present invention, a composition described in this application is described for use in cleaning, disinfecting and/or sterilizing a microbial infection in a patient. In other aspects, according to the present invention, the use of the described composition for cleaning, disinfecting and/or sterilizing a microbial infection in a patient is described. In other aspects, the present invention describes the use of the disclosed composition for the manufacture of a medicament for cleaning, disinfecting and/or sterilizing a microbial infection in a patient.

Detailed description of the invention

Disinfection and sterilization are necessary to ensure that the medical device does not transmit infectious pathogens to the patient. During manufacture, a medical device for human or animal care should be cleaned in accordance with implantation standards before coating or packaging. After use, the medical device should be cleaned, disinfected, and/or sterilized to prevent the spread of nosocomial (treatment-induced) infections to the next patient.

With regard to cleaning, disinfection and/or sterilization, a medical device can be classified as a critical device, a semi-critical device, or a non-critical device. A critical device is a device that must be sterile as it may be associated with a high risk of infection, as the medical device is inserted into a sterile tissue or vascular system and any microbial contamination can transmit disease. Non-limiting examples of a medical device that is a critical device include

- 2 042144 include a surgical instrument, a catheter such as, for example, a cardiac or urinary catheter, an implant, an ECMO (extracorporeal membrane oxygenation) heart-lung machine, and an ultrasound probe for use in sterile body cavities.

A semi-critical device is a medical device that is brought into contact with a mucous membrane or damaged skin. Although the medical device must not contain any form of microorganism, a small amount of bacterial spores is acceptable because the mucosa or damaged skin is usually resistant to infection by common bacterial spores but susceptible to other organisms such as bacteria, mycobacteria and viruses. Thus, a medical device classified as a semi-critical device should be minimally subjected to high-level disinfection using chemical disinfecting agents, since this procedure completely destroys all microorganisms in or on the medical device, with the exception of a small number of bacterial spores. Non-limiting examples of a medical device that is a semi-critical device include respiratory therapy equipment, anesthesia equipment, an endoscope such as, for example, a gastrointestinal endoscope, a bronchoscope or nasopharyngoscope, an arthroscope, a laparoscope, a laryngoscope blade, an esophageal manometry probe , cystoscope, spirometer, anorectal manometry catheter, in vitro fertilization instruments and a diaphragm adjustment ring.

A non-critical device is a medical device that is brought into contact with intact skin, but not with a mucous membrane. Intact skin acts as an effective barrier to most microorganisms; therefore, the sterility of a device brought into contact with intact skin is not critical. In fact, no risk of transmission of infectious agents to patients through non-critical devices has been reported when non-critical devices are used, unless they result in contact with broken skin and/or mucous membranes. Unlike critical and some semi-critical devices, most reusable non-critical devices can be decontaminated on site without having to be moved to a central processing area. Thus, a medical device classified as a non-critical device can be cleaned with a low level disinfectant. Non-critical devices can be divided into non-critical patient care devices and non-critical environmental surface treatment devices. Non-limiting examples of a medical device that is a non-critical patient care device include a medical vessel, a blood pressure cuff, crutches, and a computer. Non-limiting examples of a non-critical environmental surface treatment device include bedside fixtures, eating utensils, bedside tables, furniture, and floors.

The composition described in this application is suitable for cleaning, disinfecting and/or sterilizing a medical device. In one aspect, according to said embodiment, the composition described herein is suitable for cleaning, disinfecting and/or sterilizing a heat-sensitive medical device. In another aspect, according to said embodiment, the composition described herein is suitable for cleaning, disinfecting and/or sterilizing a medical device classified as a semi-critical device or a non-critical device.

The composition described in this application may contain hypochlorous acid. Hypochlorous acid is a weak acid with the chemical formula HOCl, while its molecular formula is written as HClO. As shown in Formula I, hypochlorous acid is a simple molecule with a central oxygen atom linked to chlorine and hydrogen atoms by single bonds and has a molecular weight of 52.46 g/mol.

n CI (I)

Hypochlorous acid is a colorless solution and its exact physical properties are variable depending on the concentration of hypochlorous acid in the solution. Hypochlorous acid reacts with bases to form salts called hypochlorites. For example, sodium hypochlorite (NaOCl), the active ingredient in bleach, is formed by reacting hypochlorous acid with sodium hydroxide. Hypochlorous acid also readily interacts with various organic molecules and biomolecules.

The hypochlorous acid solution can be obtained, for example, by dissolving chlorine in water, hydrolyzing chlorine gas, electrolysing a saline solution, or acidifying hypochlorite. For example, stable hypochlorous salts such as, for example, alkali metal hypochlorites such as sodium hypochlorite, calcium hypochlorite, potassium hypochlorite, lithium hypochlorite and magnesium hypochlorite can be prepared by dissolving chlorine gas in an aqueous alkali metal hydroxide solution such as sodium hydroxide solution. , calcium hydroxide solution, potassium hydroxide solution, lithium hydroxide solution or magnesium hydroxide solution, respectively.

Hypochlorous acid can also be obtained by dissolving dichlorine monoxide in water. IN

- 3 042144 as another example, hypochlorous acid can also be obtained by electrolytic treatment of saline. In one method, electric current is applied to a three-chamber cell containing a cathode chamber, an anode chamber, and a central saline chamber located between the other two chambers, all chambers being separated by a semi-permeable membrane. During electrolysis, sodium chloride (NaCl) dissociates into negatively charged chloride (Cl ^- ) and positively charged sodium (Na ⁺ ). In this case, water dissociates into hydroxide ions (OH ^- ) and hydrogen (H ⁺ ). Negatively charged ions of chlorine (Cl ^- ) and hydroxide (OH ^- ) move to the anode, losing electrons and forming hypochlorous acid (HOCl), as well as hypochlorite ions (OCl ^- ) and gaseous oxygen (O ₂ ) and chlorine (Cl ₂ ). Then, the reduction water containing hypochlorous acid is fed into the collection chamber for subsequent use. Methods for producing hypochlorous acid are described, for example, in US patent No. 3914397, 4190638, 4908215, 5322677, 6426066, 7323118, 8062500, 8945630, 9168318 and 9486479, each of which is incorporated into this application in its entirety by reference.

In one embodiment, the composition described in this application contains an amount of hypochlorous acid, which gives the desired beneficial effect of the composition described in this application. In aspects according to the specified implementation variant, the composition described in this application contains hypochlorous acid in an amount of, for example, about 0.00005%, about 0.00006%, about 0.00007%, about 0.000075%, about 0.00008% , about 0.00009%, about 0.0001%, about 0.0005%, about 0.001%, about 0.0015%, about 0.002%, about 0.0025%, about 0.003%, about 0.0035%, about 0.004%, about 0.0045%, about 0.005%, about 0.0055%, about 0.006%, about 0.007%, about 0.0075%, about 0.008%, about 0.009%, about 0.01%, about 0, 02%, about 0.03%, about 0.04%, or about 0.05% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains hypochlorous acid in an amount, for example, at least 0.00005%, at least 0.00006%, at least 0.00007%, at least 0 .000075%, at least 0.00008%, at least 0.00009%, at least 0.0001%, at least 0.0005%, at least 0.001%, at least 0.0015%, at least 0.002%, at least 0.0025%, at least 0.003%, at least 0.0035%, at least 0.004%, at least 0.0045%, at least 0.005%, at least at least 0.0055%, at least 0.006%, at least 0.007%, at least 0.0075%, at least 0.008%, at least 0.009%, at least 0.01%, at least 0 02%, at least 0.03%, at least 0.04% or at least 0.05% by weight of the composition. In other aspects, according to the specified embodiment, the composition described in this application contains hypochlorous acid in an amount, for example, not more than 0.00005%, not more than 0.00006%, not more than 0.00007%, not more than 0.000075%, not more than 0.00008%, not more than 0.00009%, not more than 0.0001%, not more than 0.0005%, not more than 0.001%, not more than 0.0015%, not more than 0.002%, not more than 0.0025 %, max 0.003%, max 0.0035%, max 0.004%, max 0.0045%, max 0.005%, max 0.0055%, max 0.006%, max 0.007%, max 0.0075%, not more than 0.008%, not more than 0.009%, not more than 0.01%, not more than 0.02%, not more than 0.03%, not more than 0.04% or not more than 0.05% of weight of the composition. In other aspects, according to the specified embodiment, the composition described in this application contains hypochlorous acid in an amount, for example, from about 0.00075% to about 0.002%, from about 0.00075% to about 0.003%, from about 0.00075% to about 0.004%, from about 0.00075% to about 0.005%, from about 0.00075% to about 0.006%, from about 0.00075% to about 0.007%, from about 0.00075% to about 0.008%, from about 0.00075% to about 0.009%, from about 0.00075% to about 0.01%, from about 0.001% to about 0.002%, from about 0.001% to about 0.003%, from about 0.001% to about 0.004%, from about 0.001% to about 0.005%, from about 0.001% to about 0.006%, from about 0.001% to about 0.007%, from about 0.001% to about 0.008%, from about 0.001% to about 0.009%, from about 0.001% to about 0.01%, from about 0.002% to about 0.003%, from about 0.002% to about 0.004%, from about 0.002% to about 0.005%, from example but 0.002% to about 0.006%, about 0.002% to about 0.007%, about 0.002% to about 0.008%, about 0.002% to about 0.009%, or about 0.002% to about 0.01% by weight of the composition.

In other aspects, according to the specified embodiment, the composition described in this application contains hypochlorous acid in an amount, for example, about 0.01%, about 0.02%, about 0.025%, about 0.03%, about 0.04%, about 0.05%, about 0.06%, about 0.07%, about 0.075%, about 0.08%, about 0.09%, about 0.10%, about 0.11%, about 0.12 %, about 0.13%, about 0.14% or about 0.15% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains hypochlorous acid in an amount, for example, at least 0.01%, at least 0.02%, at least 0.025%, at least 0.03 %, at least 0.04%, at least 0.05%, at least 0.06%, at least 0.07%, at least 0.075%, at least 0.08%, at least at least 0.09%, at least 0.10%, at least 0.11%, at least 0.12%, at least 0.13%, at least 0.14%, or at least 0 .15% by weight of the composition. In other aspects

- 4 042144 according to the specified embodiment, the composition described in this application contains hypochlorous acid in an amount, for example, not more than 0.01%, not more than 0.02%, not more than 0.025%, not more than 0.03%, not more 0.04%, max 0.05%, max 0.06%, max 0.07%, max 0.075%, max 0.08%, max 0.09%, max 0.10 %, not more than 0.11%, not more than 0.12%, not more than 0.13%, not more than 0.14% or not more than 0.15% by weight of the composition. In other aspects, according to the specified embodiment, the composition described in this application contains hypochlorous acid in an amount, for example, from about 0.01% to about 0.02%, from about 0.01% to about 0.03%, from about 0.01% to about 0.04%, about 0.01% to about 0.05%, about 0.01% to about 0.06%, about 0.01% to about 0.07%, from about 0.01% to about 0.08%, from about 0.01% to about 0.09%, from about 0.01% to about 0.10%, from about 0.01% to about 0.11 %, from about

0.01% to about 0.12%, about 0.01% to about 0.13%, about 0.01% to about

0.14%, from about 0.01% to about 0.15%, from about 0.02% to about 0.03%, from about

0.02% to about 0.04%, about 0.02% to about 0.05%, about 0.02% to about

0.06%, about 0.02% to about 0.07%, about 0.02% to about 0.08%, from about

0.02% to about 0.09%, about 0.02% to about 0.10%, about 0.02% to about

0.11%, about 0.02% to about 0.12%, about 0.02% to about 0.13%, from about

0.02% to about 0.14%, about 0.02% to about 0.15%, about 0.03% to about

0.04%, from about 0.03% to about 0.05%, from about 0.03% to about 0.06%, from about

0.03% to about 0.07%, about 0.03% to about 0.08%, about 0.03% to about

0.09%, from about 0.03% to about 0.10%, from about 0.03% to about 0.11%, from about

0.03% to about 0.12%, about 0.03% to about 0.13%, about 0.03% to about

0.14%, from about 0.03% to about 0.15%, from about 0.04% to about 0.05%, from about

0.04% to about 0.06%, about 0.04% to about 0.07%, about 0.04% to about

0.08%, about 0.04% to about 0.09%, about 0.04% to about 0.10%, from about

0.04% to about 0.11%, about 0.04% to about 0.12%, about 0.04% to about

0.13%, from about 0.04% to about 0.14%, from about 0.04% to about 0.15%, from about

0.05% to about 0.06%, about 0.05% to about 0.07%, about 0.05% to about

0.08%, about 0.05% to about 0.09%, about 0.05% to about 0.10%, from about

0.05% to about 0.11%, about 0.05% to about 0.12%, about 0.05% to about

0.13%, from about 0.05% to about 0.14%, from about 0.05% to about 0.15%, from about

0.06% to about 0.07%, about 0.06% to about 0.08%, about 0.06% to about

0.09%, from about 0.06% to about 0.10%, from about 0.06% to about 0.11%, from about

0.06% to about 0.12%, about 0.06% to about 0.13%, about 0.06% to about

0.14%, from about 0.06% to about 0.15%, from about 0.07% to about 0.08%, from about

0.07% to about 0.09%, about 0.07% to about 0.10%, about 0.07% to about

0.11%, about 0.07% to about 0.12%, about 0.07% to about 0.13%, from about

0.07% to about 0.14%, about 0.07% to about 0.15%, about 0.08% to about

0.09%, from about 0.08% to about 0.10%, from about 0.08% to about 0.11%, from about

0.08% to about 0.12%, about 0.08% to about 0.13%, about 0.08% to about

0.14%, from about 0.08% to about 0.15%, from about 0.09% to about 0.10%, from about

0.09% to about 0.11%, about 0.09% to about 0.12%, about 0.09% to about

0.13%, from about 0.09% to about 0.14%, from about 0.09% to about 0.15%, from about

0.10% to about 0.11%, about 0.10% to about 0.12%, about 0.10% to about

0.13%, about 0.10% to about 0.14%, about 0.10% to about 0.15%, from about

0.11% to about 0.12%, about 0.11% to about 0.13%, about 0.11% to about

0.14%, from about 0.11% to about 0.15%, from about 0.12% to about 0.13%, from about

0.12% to about 0.14%, about 0.12% to about 0.15%, about 0.13% to about

0.14%, from about 0.13% to about 0.15%, or from about 0.14% to about 0.15%, by weight of the composition.

In aspects according to the specified embodiment, the composition described in the invention contains hypochlorous acid in an amount of, for example, 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35ppm 0.40ppm 0.45ppm 0.50ppm 0.55ppm 0.60ppm 0.65ppm 0.70ppm 0.75ppm 0.80ppm 0.85ppm, 0.90ppm, 0.95ppm, 1ppm, 5ppm, 10ppm, 15ppm, 20ppm, 25ppm, 30ppm, 35ppm, 40ppm, 45ppm, 50ppm, 55ppm , 60ppm, 65ppm, 70ppm, 75ppm, 80ppm, 85ppm, 90ppm, 95ppm, 100ppm,

125ppm, 150ppm, 175ppm, 200ppm, 225ppm, 250ppm, 275ppm, 300ppm, 325ppm, 350ppm, 375ppm

400ppm, 425ppm, 450ppm, 475ppm, 500ppm, 525ppm, 550ppm, 575ppm, 600ppm, 625ppm, 650ppm,

675ppm, 700ppm, 725ppm, 750ppm, 775ppm, 800ppm, 825ppm, 850ppm, 875ppm, 900ppm, 925ppm,

950ppm 975ppm 1000ppm 1025ppm 1050ppm 1075ppm 1100ppm 1125ppm 1150ppm 1175ppm 1200ppm 1225ppm 1250ppm 1275ppm 1300ppm , 1375ppm, 1400ppm, 1425ppm, 1450ppm, 1475ppm or 1500ppm. In other aspects, according to the specified implementation variant, the composition described in this application contains hypochlorous acid in an amount, for example, at least 0.05 ppm, at least 0.10 ppm, at least 0.20 ppm, at least 0 ,thirty

- 5 042144 ppm, at least 0.40 ppm, at least 0.50 ppm, at least 0.60 ppm, at least 0.70 ppm, at least 0.80 ppm, at least 0, 90 ppm, at least 1 ppm, at least 10 ppm, at least 20 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, at least 60 ppm, at least 70 ppm , at least 80 ppm, at least 90 ppm, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least 200 ppm, at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least 625 ppm, at least 650 ppm , at least e 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm, at least 850 ppm, at least 875 ppm, at least 900 ppm, at least 925 ppm, at least 950 ppm, at least 975 ppm, at least 1000 ppm, at least 1025 ppm, at least 1050 ppm, at least 1075 ppm, at least 1100 ppm, at least 1125 ppm, at least 1150 ppm, at least 1175 ppm, at least 1200 ppm, at least 1225 ppm, at least 1250 ppm, at least 1275 ppm, at least at least 1300 ppm, at least 1325 ppm, at least 1350 ppm, at least 1375 ppm, at least 1400 ppm, at least 1425 ppm, at least 1450 ppm, at least 1475 ppm, or at least 1500 ppm In other aspects, according to the specified implementation variant, the composition described in this application contains hypochlorous acid in an amount, for example, not more than 0.05 ppm, not more than 0.10 ppm, not more than 0.20 ppm, not more than 0.30 ppm, 0.40ppm max, 0.50ppm max, 0.60ppm max, 0.70ppm max, 0.80ppm max, 0.90ppm max, 1ppm max, 10ppm max ppm, max 20 ppm, max 30 ppm, max 40 ppm, max 50 ppm, max 60 ppm, max 70 ppm, max 80 ppm, max 90 ppm, max 100 ppm, max 125 ppm, max 150 ppm, max 175 ppm, max 200 ppm, max 225 ppm, max 250 ppm, max 275 ppm, max 300 ppm, max 325 ppm, max 350 ppm, max 375 400ppm max, 425ppm max, 450ppm max, 475ppm max, 500ppm max, 525ppm max, 550ppm max, 575ppm max, 600ppm max, 625ppm max ppm, no more than 650 ppm, no more than 675 ppm, no more than 700 ppm, no more than 725 ppm, no more than 750 ppm, 775ppm max, 800ppm max, 825ppm max, 850ppm max, 875ppm max, 900ppm max, 925ppm max, 950ppm max, 975ppm max, 1000ppm max, 1025ppm max, 1050ppm max, 1075ppm max, 1100ppm max, 1125ppm max, 1150ppm max, 1175ppm max, 1200ppm max, 1225ppm max, 1250ppm max, 1275ppm max, 1300ppm max, 1325ppm max, 1350ppm max, 1375ppm max, 1400ppm max, 1425ppm max, 1450ppm max, 1475ppm max, or 1500ppm max.

In other aspects, according to the specified embodiment, the composition described in this application contains hypochlorous acid in an amount, for example, from about 0.5 ppm to about 20 ppm, from about 0.5 ppm to about 25 ppm, from about 0.5 ppm to about 30 ppm, from about 0.5 ppm to about 35 ppm, from about 0.5 ppm to about 40 ppm, from about 0.5 ppm to about 45 ppm, from about 0.5 ppm to about 50 ppm, from about 0.5 ppm to about 55 ppm, from about 0.5 ppm to about 60 ppm, from about 0.5 ppm to about 65 ppm, from about 0.5 ppm to about 70 ppm, from about 0.5 ppm to about 75 ppm, about 0.5 ppm to about 80 ppm, about 0.5 ppm to about 85 ppm, about 0.5 ppm to about 90 ppm, about 0.5 ppm to about 95 ppm, from about 0.5 ppm to about 100 ppm, about 0.75 ppm to about 20 ppm, about 0.75 ppm to about 25 ppm, about 0.75 ppm to about 30 ppm, about 0.75 ppm to about 35ppm from about 0.75 ppm to about 40 ppm, from about 0.75 ppm to about 45 ppm, from about 0.75 ppm to about 50 ppm, from about 0.75 ppm to about 55 ppm, from about 0.75 ppm to about 60 ppm, from about 0.75 ppm to about 65 ppm, from about 0.75 ppm to about 70 ppm, from about 0.75 ppm to about 75 ppm, from about 0.75 ppm to about 80 ppm, from about 0.75 ppm to about 85 ppm, about 0.75 ppm to about 90 ppm, about 0.75 ppm to about 95 ppm, about 0.75 ppm to about 100 ppm, about 1 ppm to about 20 ppm, from about 1 ppm to about 25 ppm, from about 1 ppm to about 30 ppm, from about 1 ppm to about 35 ppm, from about 1 ppm to about 40 ppm, from about 1 ppm to about 45 ppm, from about 1 ppm to about 50 ppm, about 1 ppm to about 55 ppm, about 1 ppm to about 60 ppm, about 1 ppm to about 65 ppm, about 1 ppm to about 70 ppm, about 1 ppm to about 75 ppm , from example but 1 ppm to about 80 ppm, about 1 ppm to about 85 ppm, about 1 ppm to about 90 ppm, about 1 ppm to about 95 ppm, about 1 ppm to about 100 ppm, about 5 ppm to about 20 ppm, about 5 ppm to about 25 ppm, about 5 ppm to about 30 ppm, about 5 ppm to about 35 ppm, about 5 ppm to about 40 ppm, about 5 ppm to about 45 ppm, from about 5 ppm to about 50 ppm, about 5 ppm to about 55 ppm, about 5 ppm to about 60 ppm, about 5 ppm to about 65 ppm, about 5 ppm to at

- 6 042144 measured 70 ppm, about 5 ppm to about 75 ppm, about 5 ppm to about 80 ppm, about 5 ppm to about 85 ppm, about 5 ppm to about 90 ppm, about 5 ppm to about 95 ppm, from about 5 ppm to about 100 ppm, from about 10 ppm to about 20 ppm, from about 10 ppm to about 25 ppm, from about 10 ppm to about 30 ppm, from about 10 ppm to about 35 ppm, from about 10 ppm to about 40 ppm, about 10 ppm to about 45 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 55 ppm, about 10 ppm to about 60 ppm, about 10 ppm to about 65 ppm , from about 10 ppm to about 70 ppm, from about 10 ppm to about 75 ppm, from about 10 ppm to about 80 ppm, from about 10 ppm to about 85 ppm, from about 10 ppm to about 90 ppm, from about 10 ppm to about 95 ppm or from about 10 ppm to about 100 ppm.

In other aspects, according to the specified embodiment, the composition described in this application contains hypochlorous acid in an amount, for example, from about 1 ppm to about 25 ppm, from about 1 ppm to about 50 ppm, from about 1 ppm to about 75 ppm, from about 1 ppm to about 100 ppm, about 1 ppm to about 125 ppm, about 1 ppm to about 150 ppm, about 1 ppm to about 175 ppm, about 1 ppm to about 200 ppm, about 1 ppm to about 225 ppm, from about 1 ppm to about 250 ppm, from about 1 ppm to about 275 ppm, from about 1 ppm to about 300 ppm, from about 1 ppm to about 325 ppm, from about 1 ppm to about 350 ppm, from about 1 ppm to about 375 ppm, about 1 ppm to about 400 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 100 ppm, from about 10 ppm to about 125 ppm, from about 10 ppm to approx. 150 ppm, from about 10 ppm to about 175 ppm, from about 10 ppm to about 200 ppm, from about 10 ppm to about 225 ppm, from about 10 ppm to about 250 ppm, from about 10 ppm to about 275 ppm, from about 10 ppm to about 300 ppm, about 10 ppm to about 325 ppm, about 10 ppm to about 350 ppm, about 10 ppm to about 375 ppm, about 10 ppm to about 400 ppm, about 25 ppm to about 50 ppm, about 25 ppm to about 75 ppm, about 25 ppm to about 100 ppm, about 25 ppm to about 125 ppm, about 25 ppm to about 150 ppm, about 25 ppm to about 175 ppm, from about 25 ppm to about 200 ppm, about 25 ppm to about 225 ppm, about 25 ppm to about 250 ppm, about 25 ppm to about 275 ppm, about 25 ppm to about 300 ppm, about 25 ppm to about 325 ppm, from about 25 ppm to about 350 ppm, from about 25 ppm to about 375 ppm, from about 25 ppm to but 400 ppm, from about 50 ppm to about 75 ppm, from about 50 ppm to about 100 ppm, from about 50 ppm to about 125 ppm, from about 50 ppm to about 150 ppm, from about 50 ppm to about 175 ppm, from about 50 ppm to about 200 ppm, about 50 ppm to about 225 ppm, about 50 ppm to about 250 ppm, about 50 ppm to about 275 ppm, about 50 ppm to about 300 ppm, about 50 ppm to about 325 ppm, about 50 ppm to about 350 ppm, about 50 ppm to about 375 ppm, about 50 ppm to about 400 ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125 ppm, from about 75 ppm to about 150 ppm, about 75 ppm to about 175 ppm, about 75 ppm to about 200 ppm, about 75 ppm to about 225 ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275 ppm, about 75 ppm to about 300 ppm, about 75 ppm to about 325 ppm, about 75 ppm to about about 350 ppm, from about 75 ppm to about 375 ppm, from about 75 ppm to about 400 ppm, from about 100 ppm to about 125 ppm, from about 100 ppm to about 150 ppm, from about 100 ppm to about 175 ppm, from about 100 ppm to about 200 ppm, about 100 ppm to about 225 ppm, about 100 ppm to about 250 ppm, about 100 ppm to about 275 ppm, about 100 ppm to about 300 ppm, about 100 ppm to about 325 ppm, about 100 ppm to about 350 ppm, about 100 ppm to about 375 ppm, about 100 ppm to about 400 ppm, about 150 ppm to about 175 ppm, about 150 ppm to about 200 ppm, from about 150 ppm to about 225 ppm, about 150 ppm to about 250 ppm, about 150 ppm to about 275 ppm, about 150 ppm to about 300 ppm, about 150 ppm to about 325 ppm, about 150 ppm to about 350 ppm, from about 150 ppm to about 375 ppm, from about 150 ppm to about 400 ppm, from approx. 200 ppm to about 225 ppm, about 200 ppm to about 250 ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300 ppm, about 200 ppm to about 325 ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375 ppm, about 200 ppm to about 400 ppm, about 250 ppm to about 275 ppm, about 250 ppm to about 300 ppm, about 250 ppm to about 325 ppm, from about 250 ppm to about 350 ppm, about 250 ppm to about 375 ppm, about 250 ppm to about 400 ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375 ppm, about 300 ppm to about 400 ppm, about 350 ppm to about 375 ppm, about 350 ppm to about 400 ppm, or about 375 ppm to about 400 ppm.

In other aspects, according to the specified implementation variant, the composition described in this

- 7 042144 application, contains hypochlorous acid in an amount, for example, from about 400 ppm to about 500 ppm, from about 400 ppm to about 600 ppm, from about 400 ppm to about 700 ppm, from about 400 ppm to about 800 ppm, from about 400 ppm to about 900 ppm, about 400 ppm to about 1000 ppm, about 400 ppm to about 1100 ppm, about 400 ppm to about 1200 ppm, about 400 ppm to about 1300 ppm, about 400 ppm to about 1400 ppm, about 400 ppm to about 1500 ppm, about 500 ppm to about 600 ppm, about 500 ppm to about 700 ppm, about 500 ppm to about 800 ppm, about 500 ppm to about 900 ppm, from about 500 ppm to about 1000 ppm, about 500 ppm to about 1100 ppm, about 500 ppm to about 1200 ppm, about 500 ppm to about 1300 ppm, about 500 ppm to about 1400 ppm, about 500 ppm to about 1500 ppm, from about 600 ppm to about 700 ppm, from about 600 ppm to about 800 ppm, about 600 ppm to about 900 ppm, about 600 ppm to about 1000 ppm, about 600 ppm to about 1100 ppm, about 600 ppm to about 1200 ppm, about 600 ppm to about 1300 ppm, from about 600 ppm to about 1400 ppm, from about 600 ppm to about 1500 ppm, from about 700 ppm to about 800 ppm, from about 700 ppm to about 900 ppm, from about 700 ppm to about 1000 ppm, from about 700 ppm to about 1100 ppm, from about 700 ppm to about 1200 ppm, from about 700 ppm to about 1300 ppm, from about 700 ppm to about 1400 ppm, from about 700 ppm to about 1500 ppm, from about 800 ppm to about 900 ppm , from about 800 ppm to about 1000 ppm, from about 800 ppm to about 1100 ppm, from about 800 ppm to about 1200 ppm, from about 800 ppm to about 1300 ppm, from about 800 ppm to about 1400 ppm, from about 800 ppm up to about 1500 ppm, from about 900 ppm to about 1000 ppm, from approx. 900 ppm to about 1100 ppm, about 900 ppm to about 1200 ppm, about 900 ppm to about 1300 ppm, about 900 ppm to about 1400 ppm, about 900 ppm to about 1500 ppm, about 1000 ppm to about 1100 ppm, from about 1000 ppm to about 1200 ppm, from about 1000 ppm to about 1300 ppm, from about 1000 ppm to about 1400 ppm, from about 1000 ppm to about 1500 ppm, from about 1100 ppm to about 1200 ppm, from about 1100 ppm to about 1300 ppm, from about 1100 ppm to about 1400 ppm, from about 1100 ppm to about 1500 ppm, from about 1200 ppm to about 1300 ppm, from about 1200 ppm to about 1400 ppm, from about 1200 ppm to about 1500 ppm, from about 1300 ppm to about 1400 ppm, from about 1300 ppm to about 1500 ppm, or from about 1400 ppm to about 1500 ppm.

The concentration of hypochlorous acid in a solution can be described as the content of free active chlorine in parts per million. Hypochlorous acid is in equilibrium with hypochlorite ions (OCF) and dissolved chlorine gas (Cl ₂ ). The degree of equilibrium is determined mainly by the pH value of the solution. Temperature also affects the ratio of the free chlorine component. Thus, it is necessary to know the FAC (free available chlorine) and pH values to understand the amount of chlorine present as hypochlorous acid. In general, at a pH in the range of about 4.0 to about 5.6, about 100% of the available chlorine is present as HOCl. As the pH drops below about 4, the content of dissolved chlorine gas (Cl ₂ ) increases. Thus, at a pH of about 3, about 90% of the available chlorine is present as hypochlorous acid, at a pH of about 2, about 75% of the available chlorine is present as hypochlorous acid, at a pH of about 1.5, about 50% of the available chlorine is present as hypochlorous acid, while at a pH of about 1, about 25% of the available chlorine is present as hypochlorous acid. As the pH increases above about 5.6, the content of hypochlorite ions (OCl ^- ) increases. Thus, at a pH of about 6.5, about 90% of the available chlorine is present as hypochlorous acid; at a pH of about 7, about 75% of the available chlorine is present as hypochlorous acid; at a pH of about 7.5, about 50% of the available chlorine is present as hypochlorous acid. acids, while at a pH of about 8, about 25% of the available chlorine is present as hypochlorous acid.

The amount of chlorine can be measured using methods known in the art, such as the colorimetric method using DPD (Lamotte Company, Chestertown, Md.), or other known methods established by the Environmental Protection Agency. In the colorimetric method using DPD, the yellow color is formed by the reaction of free chlorine with N,N-diethyl-n-phenylenediamine (DPD) and the intensity is measured with a calibrated colorimeter that gives a ppm result. Further addition of potassium iodide turns the solution pink, allowing the total chlorine to be determined.

In aspects according to the specified embodiment, the composition described in this application contains free active chlorine in an amount, for example, 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35ppm, 0.40ppm, 0.45ppm, 0.50ppm, 0.55ppm, 0.60ppm, 0.65ppm, 0.70ppm, 0.75ppm, 0.80 ppm, 0.85 ppm, 0.90 ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm, 45 ppm, 50 ppm, 55ppm, 60ppm, 65ppm, 70ppm, 75ppm, 80ppm, 85ppm, 90ppm, 95ppm, 100ppm, 125ppm, 150ppm, 175ppm, 200ppm, 225ppm, 250ppm, 275ppm , 300ppm, 325ppm, 350ppm, 375ppm,

- 8 042144

400ppm, 425ppm, 450ppm, 475ppm, 500ppm, 525ppm, 550ppm, 575ppm, 600ppm, 625ppm, 650ppm, 675ppm, 700ppm, 725ppm, 750ppm, 775ppm, 800ppm 825ppm ppm, 1250 ppm, 1275 ppm, 1300 ppm, 1325 ppm, 1350 ppm, 1375 ppm, 1400 ppm, 1425 ppm, 1450 ppm, 1475 ppm or 1500 ppm. In other aspects, according to the specified implementation variant, the composition described in this application contains free active chlorine in an amount, for example, at least 0.05 ppm, at least 0.10 ppm, at least 0.20 ppm, at least 0.30 ppm, at least 0.40 ppm, at least 0.50 ppm, at least 0.60 ppm, at least 0.70 ppm, at least 0.80 ppm, at least 0, 90 ppm, at least 1 ppm, at least 10 ppm, at least 20 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, at least 60 ppm, at least 70 ppm , at least 80 ppm, at least 90 ppm, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least 200 ppm, at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least 625 ppm, at least 650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm, at least at least 850 ppm, at least 875 ppm, at least 900 ppm, at least 925 ppm, at least 950 ppm, at least 975 ppm, at least 1000 ppm, at least 1025 ppm, at least 1050 ppm, at least 1075 ppm, at least 1100 ppm, at least 1125 ppm, at least 1150 ppm, at least 1175 ppm, at least 1200 ppm, at least 1225 ppm, at least 1250 ppm, at least 1275 ppm, at least 1300 ppm, at least 1325 ppm, at least 1350 ppm, at least 1375 ppm, at least 1400 ppm, at least 1425 ppm, at least 1450 ppm, at least 1475 ppm, or at least 1500 ppm. In other aspects, according to the specified embodiment, the composition described in this application contains free active chlorine in an amount, for example, not more than 0.05 ppm, not more than 0.10 ppm, not more than 0.20 ppm, not more than 0.30 ppm , no more than 0.40 ppm, no more than 0.50 ppm, no more than 0.60 ppm, no more than 0.70 ppm, no more than 0.80 ppm, no more than 0.90 ppm, no more than 1 ppm, no more 10 ppm, max 20 ppm, max 30 ppm, max 40 ppm, max 50 ppm, max 60 ppm, max 70 ppm, max 80 ppm, max 90 ppm, max 100 ppm, max 125 ppm, max 150 ppm, max 175 ppm, max 200 ppm, max 225 ppm, max 250 ppm, max 275 ppm, max 300 ppm, max 325 ppm, max 350 ppm, max 375 ppm, max 400 ppm, max 425 ppm, max 450 ppm, max 475 ppm, max 500 ppm, max 525 ppm, max 550 ppm, max 575 ppm, max 600 ppm, max 625 ppm, max 650 ppm, max 675 ppm, max 700 ppm, max 725 ppm, max 750 ppm , no more than 775 ppm, no more than 800 ppm, no more than 825 ppm, no more than 850 ppm, no more than 875 ppm, no more than 900 ppm, no more than 925 ppm, no more than 950 ppm, no more than 975 ppm, no more than 1000 ppm , no more than 1025 ppm, no more than 1050 ppm, no more than 1075 ppm, no more than 1100 ppm, no more than 1125 ppm, no more than 1150 ppm, no more than 1175 ppm, no more than 1200 ppm, no more than 1225 ppm, no more than 1250 ppm , 1275 ppm or less, 1300 ppm or less, 1325 ppm or less, 1350 ppm or less, 1375 ppm or less, 1400 ppm or less, 1425 ppm or less, 1450 ppm or less, 1475 ppm or less, or 1500 ppm or less .

In other aspects, according to the specified embodiment, the composition described in this application contains free active chlorine in an amount, for example, from about 0.5 ppm to about 20 ppm, from about 0.5 ppm to about 25 ppm, from about 0.5 ppm to about 30 ppm, from about 0.5 ppm to about 35 ppm, from about 0.5 ppm to about 40 ppm, from about 0.5 ppm to about 45 ppm, from about 0.5 ppm to about 50 ppm, from about 0.5 ppm to about 55 ppm, from about 0.5 ppm to about 60 ppm, from about 0.5 ppm to about 65 ppm, from about 0.5 ppm to about 70 ppm, from about 0.5 ppm to about 75 ppm, from about 0.5 ppm to about 80 ppm, from about 0.5 ppm to about 85 ppm, from about 0.5 ppm to about 90 ppm, from about 0.5 ppm to about 95 ppm, from about 0.5 ppm to about 100 ppm, from about 0.75 ppm to about 20 ppm, from about 0.75 ppm to about 25 ppm, from about 0.75 ppm to about 30 ppm, from about 0.75 ppm to about 35ppm , from about 0.75 ppm to about 40 ppm, from about 0.75 ppm to about 45 ppm, from about 0.75 ppm to about 50 ppm, from about 0.75 ppm to about 55 ppm, from about 0.75 ppm to about 60 ppm, from about 0.75 ppm to about 65 ppm, from about 0.75 ppm to about 70 ppm, from about 0.75 ppm to about 75 ppm, from about 0.75 ppm to about 80 ppm, about 0.75 ppm to about 85 ppm, about 0.75 ppm to about 90 ppm, about 0.75 ppm to about 95 ppm, about 0.75 ppm to about 100 ppm, about 1 ppm to about 20 ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 30 ppm, about 1 ppm to about 35 ppm, about 1 ppm to about 40 ppm, about 1 ppm to about 45 ppm, from about 1 ppm to about 50 ppm, about 1 ppm to about 55 ppm, about 1 ppm to about 60 ppm, about 1 ppm to about 65 ppm, about 1 ppm to about 70 ppm, from about 1 ppm up to about 75 pp m, from about 1 ppm to about 80 ppm, from about 1 ppm to about 85 ppm, from about 1 ppm to about 90 ppm, from about 1 ppm to about 95 ppm, from about 1 ppm to about 100 ppm, from about 5 ppm to about 20 ppm, about 5 ppm to about 25 ppm, about 5 ppm to about 30 ppm, about 5 ppm to about 35 ppm, about 5 ppm to about 40 ppm, about 5 ppm to about 45 ppm , from about 5 ppm to about 50 ppm, from about 5 ppm to about 55 ppm, from about 5 ppm to about 60 ppm, from about 5 ppm to about 65 ppm, from about 5 ppm to about 70 ppm, from about 5 ppm to about 75 ppm, from about 5 ppm to about 80 ppm, from about 5 ppm to about 85 ppm, from about 5 ppm to about 90 ppm, from about 5 ppm to about 95 ppm, from about 5 ppm to about 100 ppm, from about 10 ppm to about 20 ppm, from about 10 ppm to about 25 ppm, from about 10 ppm to about 30 ppm, from about 10 ppm to about 35 ppm, from about 10 ppm to about 40 ppm, about 10 ppm to about 45 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 55 ppm, about 10 ppm to about 60 ppm, about 10 ppm to about 65 ppm, about 10 ppm to about 70 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 80 ppm, about 10 ppm to about 85 ppm, about 10 ppm to about 90 ppm, from about 10 ppm to about 95 ppm or about 10 ppm to about 100 ppm.

In other aspects, according to the specified embodiment, the composition described in this application contains free active chlorine in an amount, for example, from about 1 ppm to about 25 ppm, from about 1 ppm to about 50 ppm, from about 1 ppm to about 75 ppm, from about 1 ppm to about 100 ppm, from about 1 ppm to about 125 ppm, from about 1 ppm to about 150 ppm, from about 1 ppm to about 175 ppm, from about 1 ppm to about 200 ppm, from about 1 ppm to about 225 ppm, from about 1 ppm to about 250 ppm, from about 1 ppm to about 275 ppm, from about 1 ppm to about 300 ppm, from about 1 ppm to about 325 ppm, from about 1 ppm to about 350 ppm, from about 1 ppm to about 375 ppm, about 1 ppm to about 400 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 100 ppm, from about 10 ppm to about 125 ppm, from about 10 ppm to at 150 ppm, about 10 ppm to about 175 ppm, about 10 ppm to about 200 ppm, about 10 ppm to about 225 ppm, about 10 ppm to about 250 ppm, about 10 ppm to about 275 ppm, from about 10 ppm to about 300 ppm, about 10 ppm to about 325 ppm, about 10 ppm to about 350 ppm, about 10 ppm to about 375 ppm, about 10 ppm to about 400 ppm, about 25 ppm to about 50 ppm, about 25 ppm to about 75 ppm, about 25 ppm to about 100 ppm, about 25 ppm to about 125 ppm, about 25 ppm to about 150 ppm, about 25 ppm to about 175 ppm, from about 25 ppm to about 200 ppm, about 25 ppm to about 225 ppm, about 25 ppm to about 250 ppm, about 25 ppm to about 275 ppm, about 25 ppm to about 300 ppm, about 25 ppm to about 325 ppm, from about 25 ppm to about 350 ppm, from about 25 ppm to about 375 ppm, from about 25 ppm to approx. up to 400 ppm, from about 50 ppm to about 75 ppm, from about 50 ppm to about 100 ppm, from about 50 ppm to about 125 ppm, from about 50 ppm to about 150 ppm, from about 50 ppm to about 175 ppm, from about 50 ppm to about 200 ppm, about 50 ppm to about 225 ppm, about 50 ppm to about 250 ppm, about 50 ppm to about 275 ppm, about 50 ppm to about 300 ppm, about 50 ppm to about 325 ppm, about 50 ppm to about 350 ppm, about 50 ppm to about 375 ppm, about 50 ppm to about 400 ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125 ppm, from about 75 ppm to about 150 ppm, about 75 ppm to about 175 ppm, about 75 ppm to about 200 ppm, about 75 ppm to about 225 ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275 ppm, from about 75 ppm to about 300 ppm, from about 75 ppm to about 325 ppm, from about 75 ppm to but 350 ppm, from about 75 ppm to about 375 ppm, from about 75 ppm to about 400 ppm, from about 100 ppm to about 125 ppm, from about 100 ppm to about 150 ppm, from about 100 ppm to about 175 ppm, from about 100 ppm to about 200 ppm, about 100 ppm to about 225 ppm, about 100 ppm to about 250 ppm, about 100 ppm to about 275 ppm, about 100 ppm to about 300 ppm, about 100 ppm to about 325 ppm, about 100 ppm to about 350 ppm, about 100 ppm to about 375 ppm, about 100 ppm to about 400 ppm, about 150 ppm to about 175 ppm, about 150 ppm to about 200 ppm, from about 150 ppm to about 225 ppm, about 150 ppm to about 250 ppm, about 150 ppm to about 275 ppm, about 150 ppm to about 300 ppm, about 150 ppm to about 325 ppm, about 150 ppm to about 350 ppm, from about 150 ppm to about 375 ppm, from about 150 ppm to about 400 ppm, from at 200 ppm to about 225 ppm, about 200 ppm to about 250 ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300 ppm, about 200 ppm to about 325 ppm, about 200 ppm to about 350 ppm, from about 200 ppm to about 375 ppm, from about 200

- 10 042144 ppm to about 400 ppm, from about 250 ppm to about 275 ppm, from about 250 ppm to about 300 ppm, from about 250 ppm to about 325 ppm, from about 250 ppm to about 350 ppm, from about 250 ppm to about 375 ppm, from about 250 ppm to about 400 ppm, from about 300 ppm to about 325 ppm, from about 300 ppm to about 350 ppm, from about 300 ppm to about 375 ppm, from about 300 ppm to about 400 ppm, from about 350 ppm to about 375 ppm, about 350 ppm to about 400 ppm, or about 375 ppm to about 400 ppm.

In other aspects, according to the specified embodiment, the composition described in this application contains free active chlorine in an amount, for example, from about 400 ppm to about 500 ppm, from about 400 ppm to about 600 ppm, from about 400 ppm to about 700 ppm, from about 400 ppm to about 800 ppm, from about 400 ppm to about 900 ppm, from about 400 ppm to about 1000 ppm, from about 400 ppm to about 1100 ppm, from about 400 ppm to about 1200 ppm, from about 400 ppm to about 1300 ppm, from about 400 ppm to about 1400 ppm, from about 400 ppm to about 1500 ppm, from about 500 ppm to about 600 ppm, from about 500 ppm to about 700 ppm, from about 500 ppm to about 800 ppm, from about 500 ppm to about 900 ppm, about 500 ppm to about 1000 ppm, about 500 ppm to about 1100 ppm, about 500 ppm to about 1200 ppm, about 500 ppm to about 1300 ppm, about 500 ppm to about 1400 ppm, from approx. 500 ppm to about 1500 ppm, about 600 ppm to about 700 ppm, about 600 ppm to about 800 ppm, about 600 ppm to about 900 ppm, about 600 ppm to about 1000 ppm, about 600 ppm to about 1100 ppm, from about 600 ppm to about 1200 ppm, from about 600 ppm to about 1300 ppm, from about 600 ppm to about 1400 ppm, from about 600 ppm to about 1500 ppm, from about 700 ppm to about 800 ppm, from about 700 ppm to about 900 ppm, from about 700 ppm to about 1000 ppm, from about 700 ppm to about 1100 ppm, from about 700 ppm to about 1200 ppm, from about 700 ppm to about 1300 ppm, from about 700 ppm to about 1400 ppm , from about 700 ppm to about 1500 ppm, from about 800 ppm to about 900 ppm, from about 800 ppm to about 1000 ppm, from about 800 ppm to about 1100 ppm, from about 800 ppm to about 1200 ppm, from about 800 ppm up to about 1300 ppm, from about 800 ppm to about 1400 ppm, from about 800 ppm to about 1500 ppm, about 900 ppm to about 1000 ppm, about 900 ppm to about 1100 ppm, about 900 ppm to about 1200 ppm, about 900 ppm to about 1300 ppm, about 900 ppm to about 1400 ppm, from about 900 ppm to about 1500 ppm, from about 1000 ppm to about 1100 ppm, from about 1000 ppm to about 1200 ppm, from about 1000 ppm to about 1300 ppm, from about 1000 ppm to about 1400 ppm, from about 1000 ppm to about 1500 ppm, from about 1100 ppm to about 1200 ppm, from about 1100 ppm to about 1300 ppm, from about 1100 ppm to about 1400 ppm, from about 1100 ppm to about 1500 ppm, from about 1200 ppm to about 1300 ppm, from about 1200 ppm to about 1400 ppm, from about 1200 ppm to about 1500 ppm, from about 1300 ppm to about 1400 ppm, from about 1300 ppm to about 1500 ppm, or from about 1400 ppm to about 1500 ppm.

In one embodiment, the composition described in this application does not contain ozone. In another embodiment, the implementation of the composition described in this application may contain ozone.

In one embodiment, the composition described in this application does not contain hydrogen peroxide. In another embodiment, the implementation of the composition described in this application may contain hydrogen peroxide.

The composition described in this application may contain a disinfectant agent. The disinfecting agent may be one or more compounds containing a biguanide moiety or functional group, one or more aldehyde-containing compounds, or one or more organic peroxides, or any combination thereof.

The composition described in this application may contain a compound containing a guanide moiety and/or a functional group. Non-limiting examples of compounds containing a guanide moiety include an organic compound containing a biguanide functional group, a biguanide functional group, or a triguanide functional group. In one embodiment, a compound containing a guanide moiety described herein is a biguanide or a compound containing a biguanide moiety. In one aspect, according to said embodiment, the compound containing the biguanide moiety contains one, two, three, four, or five biguanide functional groups. In one aspect, according to said embodiment, the compound containing the biguanide moiety comprises Formula II

where each of R ⁵ and R ⁶ independently can be a bond, H, C, NH, NH ₂ , C _1-10 alkyl, C _1-10 alkane, C _1-10 alkyne, 5- or 6-membered aromatic ring, optionally substituted with halogen; and n

- 11 042144 is 1-5. Halogen is F, Br, Cl, I and At. Non-limiting examples of compounds containing a biguanide moiety include polyhexamethylene biguanide (PHMB), polyaminopropyl biguanide (PAPB), 1,1'-(1,6-hexanediyl)bis{2-[N'-(2-ethylhexyl)carbamimidoyl]guanidine} (alexidine ), chlorhexidine and chlorhexidine gluconate.

In one embodiment, the guanidine moiety-containing compound described herein is a biguanidine or a compound containing a biguanidine moiety. In one aspect, according to said embodiment, the compound containing the biguanidine moiety contains one, two, three, four, or five biguanidine functional groups. In one aspect, according to said embodiment, the compound containing the biguanidine moiety comprises formula III

where each of R ⁷ and R ⁸ independently can be a bond, H, C, NH, NH2, C _1-10 alkyl, C _1-10 alkane, C _1-10 alkyne, 5- or 6-membered aromatic ring, optionally substituted with halogen; and n is 1-5. Halogen is F, Br, Cl, I and At. In one embodiment, the guanide moiety-containing compound described herein is a triguanide or a triguanide moiety-containing compound. In one aspect, according to said embodiment, the compound containing the triguanide moiety contains one, two, three, four, or five triguanide functional groups. In one aspect, according to said embodiment, the compound containing the triguanide moiety comprises formula IV

where each of R ⁹ and R ¹⁰ independently can be a bond, H, C, NH, NH2, C _1-10 alkyl, C _1-10 alkane, C _1-10 alkyne, 5- or 6-membered aromatic ring, optionally substituted with halogen; and n is 1-5. Halogen is F, Br, Cl, I and At.

In one embodiment, the composition described in this application contains one compound containing a guanide moiety. In another embodiment, the composition described in this application contains many compounds containing a guanide moiety. In aspects according to said embodiment, the composition described herein comprises, for example, one or more compounds containing a guanide moiety, two or more compounds containing a guanide moiety, three or more compounds containing a guanide moiety, four or more compounds containing a guanide moiety; or five or more compounds containing a guanide moiety. In other aspects, according to the specified implementation variant, the composition described in this application contains, for example, only one compound containing a guanide moiety, no more than two compounds containing a guanide moiety, no more than three compounds containing a guanide moiety, no more than four compounds containing guanide fragment, or not more than five compounds containing a guanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains, for example, from 1 to 2 compounds containing a guanide fragment, from 1 to 3 compounds containing a guanide fragment, from 1 to 4 compounds containing a guanide fragment, from 1 up to 5 compounds containing a guanide fragment, from 2 to 3 compounds containing a guanide fragment, from 2 to 4 compounds containing a guanide fragment, from 2 to 5 compounds containing a guanide fragment, from 3 to 4 compounds containing a guanide fragment, from 3 up to 5 compounds containing a guanide fragment, or from 4 to 5 compounds containing a guanide fragment.

You can use any amount described in this application of the connection containing guanide fragment, provided that this amount is suitable for use in the methods described in this application. In aspects according to the specified implementation variant, the composition described in this application contains a compound containing a guanide fragment, in an amount of, for example, about 0.01%, about 0.05%, about 0.075%, about 0.1%, about 0, 2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1.0% , about 1.5%, about 2.0%, about 2.5%, about 3.0%, about 4.0%, about 5.0%, about 6.0%, about 7.0%, about 7.5%, about 8.0%, about 9.0%, about 10.0%, about 11%, about 12%, about 13%, about 14%, about 15%, about 20%, about 25% or about 30% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains a compound containing a guanide fragment, in an amount of, for example, at least 0.01%, at least 0.05%, at least 0.075%,

- 12 042144 at least 0.1%, at least 0.25%, at least 0.5%, at least 0.75%, at least 1.0%, at least 1.5%, at least 2.0%, at least 2.5%, at least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%, at least at least 7.0%, at least 7.5%, at least 8.0%, at least 9.0%, at least 10.0%, at least 11%, at least 12%, at least 13%, at least 14%, at least 15%, at least 20%, at least 25% or at least 30% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains a compound containing a guanide fragment, in an amount, for example, not more than 0.01%, not more than 0.05%, not more than 0.075%, not more than 0.1 %, no more than 0.25%, no more than 0.5%, no more than 0.75%, no more than 1.0%, no more than 1.5%, no more than 2.0%, no more than 2.5% , not more than 3.0%, not more than 4.0%, not more than 5.0%, not more than 6.0%, not more than 7.5%, not more than 8.0%, not more than 9.0%, not more than 10.0%, not more than 11%, not more than 12%, not more than 13%, not more than 14%, not more than 15%, not more than 20%, not more than 25% or not more than 30% by weight of the composition.

In other aspects, according to the specified implementation variant, the composition described in this application contains a compound containing a guanide fragment, in an amount, for example, from about 0.1% to about 0.5%, from about 0.1% to about 0.75 %, from about 0.1% to about 1.0%, from about 0.1% to about 1.5%, from about 0.1% to about 2.0%, from about 0.1% to about 2 .5%, from about 0.1% to about 3.0%, from about 0.1% to about 3.5%, from about 0.1% to about 4.0%, from about 0.1% to about 4.5%, from about 0.1% to about 5.0%, from about 0.2% to about 0.5%, from about 0.2% to about 0.75%, from about 0.2 % to about 1.0%, from about 0.2% to about 1.5%, from about 0.2% to about 2.0%, from about 0.2% to about 2.5%, from about 0 .2% to about 3.0%, from about 0.2% to about 3.5%, from about 0.2% to about 4.0%, from about 0.2% to about 4.5%, from about 0.2% to about 5.0%, from about 0.5% to about 1.0%, from about 0.5% up to about 1.5%, from about 0.5% to about 2.0%, from about 0.5% to about 2.5%, from about 0.5% to about 3.0%, from about 0, 5% to about 4.0%, about 0.5% to about 5.0%, about 0.5% to about 6.0%, about 0.5% to about 7.0%, from about 0.5% to about 8.0%, about 0.5% to about 9.0%, about 0.5% to about 10.0%, about 1.0% to about 2.5%, from about 1.0% to about 3.0%, from about 1.0% to about 4.0%, from about 1.0% to about 5.0%, from about 1.0% to about 6.0 %, from about 1.0% to about 7.0%, from about 1.0% to about 7.5%, from about 1.0% to about 8.0%, from about 1.0% to about 9 .0%, from about 1.0% to about 10.0%, from about 1.0% to about 15.0%, from about 1.0% to about 20.0%, from about 1.0% to about 25.0%, from about 1.0% to about 30.0%, from about 2.0% to about 2.5%, from about 2.0% to about 3.0%, from about 2.0 % to about 4.0%, from about 2.0% to about 5, 0%, about 2.0% to about 6.0%, about 2.0% to about 7.0%, about 2.0% to about 7.5%, about 2.0% to about 8.0%, from about 2.0% to about 9.0%, from about 2.0% to about 10.0%, from about 2.0% to about 15.0%, from about 2.0% up to about 20.0%, from about 2.0% to about 25.0%, from about 2.0% to about 30.0%, from about 5.0% to about 6.0%, from about 5, 0% to about 7.0%, from about 5.0% to about 7.5%, from about 5.0% to about 8.0%, from about 5.0% to about 9.0%, from about 5.0% to about 10.0%, from about 5.0% to about 11.0%, from about 5.0% to about 12.0%, from about 5.0% to about 13.0%, from about 5.0% to about 14.0%, from about 5.0% to about 15.0%, from about 5.0% to about 20.0%, from about 5.0% to about 25.0 %, from about 5.0% to about 30.0%, from about 6.0% to about 7.0%, from about 6.0% to about 7.5%, from about 6.0% to about 8 .0%, from about 6.0% to about 9.0%, from about 6.0% to about 10.0%, from about 6.0% to about 11.0%, from about 6.0% to about 12.0%, from about 6.0% to about 13.0% , from about 6.0% to about 14.0%, from about 6.0% to about 15.0%, from about 6.0% to about 20.0%, from about 6.0% to about 25, 0%, about 6.0% to about 30.0%, about 7.0% to about 8.0%, about 7.0% to about 9.0%, about 7.0% to about 10.0%, from about 7.0% to about 11.0%, from about 7.0% to about 12.0%, from about 7.0% to about 13.0%, from about 7.0% up to about 14.0%, from about 7.0% to about 15.0%, from about 7.0% to about 20.0%, from about 7.0% to about 25.0%, from about 7, 0% to about 30.0%, about 8.0% to about 9.0%, about 8.0% to about 10.0%, about 8.0% to about 11.0%, from about 8.0% to about 12.0%, from about 8.0% to about 13.0%, from about 8.0% to about 14.0%, from about 8.0% to about 15.0%, from about 8.0% to about 20.0 %, from about 8.0% to about 25.0%, from about 8.0% to about 30.0%, from about 9.0% to about 10.0%, from about 9.0% to about 11 .0%, from about 9.0% to about 12.0%, from about 9.0% to about 13.0%, from about 9.0% to about 14.0%, from about 9.0% to about 15.0%, from about 9.0% to about 20.0%, from about 9.0% to about 25.0%, from about 9.0% to about 30.0%, from about 10.0 % to about 11.0%, from about 10.0% to about 12.0%, from about 10.0% to about 13.0%, from about 10.0% to about 14.0%, from about 10 0% to about 15.0%, from about 10.0% to about 20.0%, from about 10.0% to about 25.0%, from about 10.0% to about 30.0%, from about 15.0% to about 20.0%, from about 15.0% to about 25.0%, from example

- 13 042144 but 15.0% to about 30.0%, from about 20.0% to about 25.0%, from about 20.0% to about 30.0%, or from about 20.0% to about 30 0% by weight of the composition.

In another embodiment, the implementation of the composition described in this application does not contain a compound containing a guanide fragment. In another embodiment, the implementation of the composition described in this application does not contain biguanide. In another embodiment, the implementation of the composition described in this application does not contain a compound containing a biguanide fragment. In another embodiment, the implementation of the composition described in this application does not contain biguanidine. In another embodiment, the implementation of the composition described in this application does not contain a compound containing a biguanidine fragment. In another embodiment, the composition described in this application does not contain triguanide. In another embodiment, the implementation of the composition described in this application does not contain a compound containing a triguanide fragment.

The composition described in this application may contain an aldehyde-containing compound. The aldehyde-containing compound may be a linear aldehyde, a branched aldehyde, a cyclic aldehyde, an aromatic aldehyde, a linear dialdehyde, a branched dialdehyde, a cyclic dialdehyde, or an aromatic dialdehyde.

An aldehyde is an organic compound containing a -CHO functional group and having a carbonyl center (carbon double bonded to oxygen) with a carbon atom also bonded to hydrogen and an R group. In one embodiment, the aldehyde described herein is a compound of formula V, wherein R ¹ may be H, C _1-6 alkyl, C _1-6 alkane, C _1-6 alkyne, C _3-6 cyclic alkyl, or C _3-6 aryl.

ABOUT

R ¹ ^^HV

Non-limiting examples of aldehydes include formaldehyde, acetaldehyde, propionaldehyde, and butyraldehyde.

Dialdehyde is an organic chemical compound with two aldehyde groups. In one embodiment, the aldehyde described herein is a compound of formula VI, where the R ² group may be a bond, C _1-6 alkyl, C _1-6 alkane, C _1-6 alkyne, C _3-6 cyclic alkyl or C _3-6 aryl.

SG R ² VI

Non-limiting examples of dialdehydes include oxaldehyde, malondialdehyde, glutaraldehyde, succinic dialdehyde, phthalaldehyde (ortho-phthalaldehyde), isophthalaldehyde (metaphthalaldehyde), and terephthalaldehyde (para-phthalaldehyde).

In one embodiment, the composition of the invention contains a single aldehyde-containing compound. In another embodiment, the composition described in this application contains many aldehyde-containing compounds. In aspects according to said embodiment, the composition of the invention comprises, for example, one or more aldehyde-containing compounds, two or more aldehyde-containing compounds, three or more aldehyde-containing compounds, four or more aldehyde-containing compounds, or five or more aldehyde-containing compounds. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, only one aldehyde-containing compound, no more than two aldehyde-containing compounds, no more than three aldehyde-containing compounds, no more than four aldehyde-containing compounds, or no more than five aldehyde-containing compounds. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, from 1 to 2 aldehyde-containing compounds, from 1 to 3 aldehyde-containing compounds, from 1 to 4 aldehyde-containing compounds, from 1 to 5 aldehyde-containing compounds, from 2 to 3 aldehyde-containing compounds, 2 to 4 aldehyde-containing compounds, 2 to 5 aldehyde-containing compounds, 3 to 4 aldehyde-containing compounds, 3 to 5 aldehyde-containing compounds, or 4 to 5 aldehyde-containing compounds.

You can use any amount described in this application aldehyde-containing compounds, provided that this amount is suitable for use in the methods described in this application. In aspects according to the specified embodiment, the composition described in this application contains an aldehyde-containing compound in an amount of, for example, about 0.01%, about 0.05%, about 0.075%, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1.0%, about 1, 5%, about 2.0%, about 2.5%, about 3.0%, about 4.0%, about 5.0%, about 6.0%, about 7.0%, about 7.5% , about 8.0%, about 9.0%, or about 10.0% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains an aldehyde-containing compound in an amount, for example, at least 0.01%, according to

- 14 042144 at least 0.05%, at least 0.075%, at least 0.1%, at least 0.25%, at least 0.5%, at least 0.75%, at least at least 1.0%, at least 1.5%, at least 2.0%, at least 2.5%, at least 3.0%, at least 4.0%, at least 5 .0%, at least 6.0%, at least 7.0%, at least 7.5%, at least 8.0%, at least 9.0%, or at least 10.0 % by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains an aldehyde-containing compound in an amount, for example, not more than 0.01%, not more than 0.05%, not more than 0.075%, not more than 0.1%, not more than 0.25%, max 0.5%, max 0.75%, max 1.0%, max 1.5%, max 2.0%, max 2.5%, max 3 .0%, not more than 4.0%, not more than 5.0%, not more than 6.0%, not more than 7.5%, not more than 8.0%, not more than 9.0% or not more than 10, 0% by weight of the composition.

In other aspects, according to the specified embodiment, the composition described in this application contains an aldehyde-containing compound in an amount, for example, from about 0.1% to about 0.5%, from about 0.1% to about 0.75%, from about 0.1% to about 1.0%, about 0.1% to about 1.5%, about 0.1% to about 2.0%, about 0.1% to about 2.5%, about 0.2% to about 0.5%, about 0.2% to about 0.75%, about 0.2% to about 1.0%, about 0.2% to about 1.5 %, from about 0.2% to about 2.0%, from about 0.2% to about 2.5%, from about 0.5% to about 1.0%, from about 0.5% to about 1 .5%, from about 0.5% to about 2.0%, from about 0.5% to about 2.5%, from about 0.5% to about 3.0%, from about 0.5% to about 4.0%, from about 0.5% to about 5.0%, from about 1.0% to about 2.5%, from about 1.0% to about 3.0%, from about 1.0 % to about 4.0%, from about 1.0% to about 5.0%, from about 1.0% to about 6 .0%, from about 1.0% to about 7.0%, from about 1.0% to about 7.5%, from about 1.0% to about 8.0%, from about 1.0% to about 9.0%, from about 1.0% to about 10.0%, from about 2.0% to about 2.5%, from about 2.0% to about 3.0%, from about 2.0 % to about 4.0%, from about 2.0% to about 5.0%, from about 2.0% to about 6.0%, from about 2.0% to about 7.0%, from about 2 0% to about 7.5%, from about 2.0% to about 8.0%, from about 2.0% to about 9.0%, from about 2.0% to about 10.0%, from about 5.0% to about 6.0%, from about 5.0% to about 7.0%, from about 5.0% to about 7.5%, from about 5.0% to about 8.0% , from about 5.0% to about 9.0%, from about 5.0% to about 10.0%, from about 5.0% to about 11.0%, from about 5.0% to about 12, 0%, from about 5.0% to about 13.0%, from about 5.0% to about 14.0%, or from about 5.0% to about 15.0% by weight of the composition.

In aspects according to the specified embodiment, the composition described in this application contains an aldehyde-containing compound in an amount of, for example, 100 ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm . ppm, 775 ppm, 800 ppm, 825 ppm, 850 ppm, 875 ppm, 900 ppm, 925 ppm, 950 ppm, 975 ppm or 1000 ppm. In other aspects, according to the specified embodiment, the composition described in this application contains an aldehyde-containing compound in an amount of, for example, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least 200 ppm, at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least at least 625 ppm, at least 650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm, at least 850 ppm, at least 875 ppm, at least 900 ppm, at least m here 925 ppm, at least 950 ppm, at least 975 ppm, or at least 1000 ppm. In other aspects, according to the specified embodiment, the composition described in this application contains an aldehyde-containing compound in an amount, for example, not more than 100 ppm, not more than 125 ppm, not more than 150 ppm, not more than 175 ppm, not more than 200 ppm, not more than 225 250ppm max, 275ppm max, 300ppm max, 325ppm max, 350ppm max, 375ppm max, 400ppm max, 425ppm max, 450ppm max, 475ppm max 500ppm max, 525ppm max, 550ppm max, 575ppm max, 600ppm max, 625ppm max, 650ppm max, 675ppm max, 700ppm max, 725ppm max 750ppm max, 775ppm max, 800ppm max, 825ppm max, 850ppm max, 875ppm max, 900ppm max, 925ppm max, 950ppm max, 975ppm max ppm or not more than 1000 ppm.

In other aspects, according to the specified embodiment, the composition described in this application contains an aldehyde-containing compound in an amount, for example, from about 100 ppm to about 200 ppm, from about 100 ppm to about 300 ppm, from about 100 ppm to about 400 ppm, from about 100 ppm to about 500 ppm, about 100 ppm to about 600 ppm, about 100 ppm to about 700 ppm, about 100 ppm to about 800 ppm, about 100 ppm to about 900 ppm, about 100 ppm to about 1000 ppm, from about 200 ppm to about

- 15 042144

300 ppm, about 200 ppm to about 400 ppm, about 200 ppm to about 500 ppm, about 200 ppm to about 600 ppm, about 200 ppm to about 700 ppm, about 200 ppm to about 800 ppm, from about 200 ppm to about 900 ppm, about 200 ppm to about 1000 ppm, about 300 ppm to about 400 ppm, about 300 ppm to about 500 ppm, about 300 ppm to about 600 ppm, about 300 ppm to about 700 ppm, from about 300 ppm to about 800 ppm, from about 300 ppm to about 900 ppm, from about 300 ppm to about 1000 ppm, from about 400 ppm to about 500 ppm, from about 400 ppm to about 600 ppm, from about 400 ppm to about 700 ppm, from about 400 ppm to about 800 ppm, from about 400 ppm to about 900 ppm, from about 400 ppm to about 1000 ppm, from about 500 ppm to about 600 ppm, from about 500 ppm to about 700 ppm , from about 500 ppm to about 800 ppm, from about 500 ppm to about 900 ppm, from about 500 ppm to about 1000 ppm, about 600 ppm to about 700 ppm, about 600 ppm to about 800 ppm, about 600 ppm to about 900 ppm, about 600 ppm to about 1000 ppm, about 700 ppm to about 800 ppm, about 700 ppm to about 900 ppm, about 700 ppm to about 1000 ppm, about 800 ppm to about 900 ppm, about 800 ppm to about 1000 ppm, or about 900 ppm to about 1000 ppm.

In another embodiment, the implementation of the composition described in this application does not contain aldehyde-containing compound.

In one embodiment, the composition described in this application contains one aldehyde. In another embodiment, the composition described in this application contains many aldehydes. In aspects according to said embodiment, the composition described herein contains, for example, one or more aldehydes, two or more aldehydes, three or more aldehydes, four or more aldehydes, or five or more aldehydes. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, only one aldehyde, no more than two aldehydes, no more than three aldehydes, no more than four aldehydes, or no more than five aldehydes. In other aspects according to the specified embodiment, the composition described in this application contains, for example, from 1 to 2 aldehydes, from 1 to 3 aldehydes, from 1 to 4 aldehydes, from 1 to 5 aldehydes, from 2 to 3 aldehydes, from 2 up to 4 aldehydes, 2 to 5 aldehydes, 3 to 4 aldehydes, 3 to 5 aldehydes, or 4 to 5 aldehydes.

You can use any amount described in this application of the aldehyde, provided that this amount is suitable for use in the methods described in this application. In aspects according to the specified implementation variant, the composition described in this application contains aldehyde in an amount of, for example, about 0.01%, about 0.05%, about 0.075%, about 0.1%, about 0.2%, about 0 .3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1.0%, about 1.5 %, about 2.0%, about 2.5%, about 3.0%, about 4.0%, about 5.0%, about 6.0%, about 7.0%, about 7.5%, about 8.0%, about 9.0%, or about 10.0% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains aldehyde in an amount, for example, at least 0.01%, at least 0.05%, at least 0.075%, at least 0.1% , at least 0.25%, at least 0.5%, at least 0.75%, at least 1.0%, at least 1.5%, at least 2.0%, at least 2.5%, at least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%, at least 7.0%, at least 7.5%, at least 8.0%, at least 9.0% or at least 10.0% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains aldehyde in an amount, for example, not more than 0.01%, not more than 0.05%, not more than 0.075%, not more than 0.1%, not more than 0 .25%, not more than 0.5%, not more than 0.75%, not more than 1.0%, not more than 1.5%, not more than 2.0%, not more than 2.5%, not more than 3, 0%, 4.0% or less, 5.0% or less, 6.0% or less, 7.5% or less, 8.0% or less, 9.0% or less, or 10.0 or less % by weight of the composition.

In other aspects, according to the specified implementation variant, the composition described in this application contains aldehyde in an amount, for example, from about 0.1% to about 0.5%, from about 0.1% to about 0.75%, from about 0 1% to about 1.0%, from about 0.1% to about 1.5%, from about 0.1% to about 2.0%, from about 0.1% to about 2.5%, from about 0.2% to about 0.5%, about 0.2% to about 0.75%, about 0.2% to about 1.0%, about 0.2% to about 1.5% , from about 0.2% to about 2.0%, from about 0.2% to about 2.5%, from about 0.5% to about 1.0%, from about 0.5% to about 1, 5%, about 0.5% to about 2.0%, about 0.5% to about 2.5%, about 0.5% to about 3.0%, about 0.5% to about 4.0%, from about 0.5% to about 5.0%, from about 1.0% to about 2.5%, from about 1.0% to about 3.0%, from about 1.0% to about 4.0%, from about 1.0% to about 5.0%, from about 1.0% to about 6.0%, from about 1.0% to about 7.0%, from about 1.0% to about 7.5%, from about 1.0% to about 8.0%, from about 1.0% to about 9.0%, from about 1, 0% to about 10.0%, about 2.0% to about 2.5%, about 2.0% to about 3.0%, about 2.0% to about 4.0%, from about 2.0% to about 5.0%, from about 2.0% to about 6.0%, from about

- 16 042144

2.0% to about 7.0%, from about 2.0% to about 7.5%, from about 2.0% to about 8.0%, from about 2.0% to about 9.0%, about 2.0% to about 10.0%, about 5.0% to about 6.0%, about 5.0% to about 7.0%, about 5.0% to about 7.5 %, from about 5.0% to about 8.0%, from about 5.0% to about 9.0%, from about 5.0% to about 10.0%, from about 5.0% to about 11 .0%, from about 5.0% to about 12.0%, from about 5.0% to about 13.0%, from about 5.0% to about 14.0%, or from about 5.0% to about 15.0% by weight of the composition.

In aspects according to the specified embodiment, the composition described in this application contains aldehyde in an amount of, for example, 100 ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm, 350ppm, 375ppm, 400ppm, 425ppm, 450ppm, 475ppm, 500ppm, 525ppm, 550ppm, 575ppm, 600ppm, 625ppm, 650ppm, 675ppm, 700ppm, 725ppm, 750ppm , 775ppm, 800ppm, 825ppm, 850ppm, 875ppm, 900ppm, 925ppm, 950ppm, 975ppm or 1000ppm. In other aspects, according to the specified embodiment, the composition described in this application contains aldehyde in an amount, for example, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least 200 ppm , at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least 625 ppm, at least 650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm , at least 850 ppm, at least 875 ppm, at least 900 ppm, at least 925 ppm, at least it is at least 950 ppm, at least 975 ppm, or at least 1000 ppm. In other aspects, according to the specified embodiment, the composition described in this application contains aldehyde in an amount, for example, not more than 100 ppm, not more than 125 ppm, not more than 150 ppm, not more than 175 ppm, not more than 200 ppm, not more than 225 ppm , no more than 250 ppm, no more than 275 ppm, no more than 300 ppm, no more than 325 ppm, no more than 350 ppm, no more than 375 ppm, no more than 400 ppm, no more than 425 ppm, no more than 450 ppm, no more than 475 ppm , 500ppm max, 525ppm max, 550ppm max, 575ppm max, 600ppm max, 625ppm max, 650ppm max, 675ppm max, 700ppm max, 725ppm max , no more than 750 ppm, no more than 775 ppm, no more than 800 ppm, no more than 825 ppm, no more than 850 ppm, no more than 875 ppm, no more than 900 ppm, no more than 925 ppm, no more than 950 ppm, no more than 975 ppm or not more than 1000 ppm.

In other aspects, according to the specified embodiment, the composition described in this application contains aldehyde in an amount, for example, from about 100 ppm to about 200 ppm, from about 100 ppm to about 300 ppm, from about 100 ppm to about 400 ppm, from about 100 ppm to about 500 ppm, about 100 ppm to about 600 ppm, about 100 ppm to about 700 ppm, about 100 ppm to about 800 ppm, about 100 ppm to about 900 ppm, about 100 ppm to about 1000 ppm, from about 200 ppm to about 300 ppm, from about 200 ppm to about 400 ppm, from about 200 ppm to about 500 ppm, from about 200 ppm to about 600 ppm, from about 200 ppm to about 700 ppm, from about 200 ppm to about 800 ppm, from about 200 ppm to about 900 ppm, from about 200 ppm to about 1000 ppm, from about 300 ppm to about 400 ppm, from about 300 ppm to about 500 ppm, from about 300 ppm to about 600 ppm , from about 300 ppm to about 700 ppm, from about 300 ppm to about 800 ppm, from about 300 ppm to about 900 ppm, from about 300 ppm to about 1000 ppm, from about 400 ppm to about 500 ppm, from about 400 ppm to about 600 ppm, from about 400 ppm to about 700 ppm, from about 400 ppm to about 800 ppm, from about 400 ppm to about 900 ppm, from about 400 ppm to about 1000 ppm, from about 500 ppm to about 600 ppm, from about 500 ppm to about 700 ppm , from about 500 ppm to about 800 ppm, from about 500 ppm to about 900 ppm, from about 500 ppm to about 1000 ppm, from about 600 ppm to about 700 ppm, from about 600 ppm to about 800 ppm, from about 600 ppm up to about 900 ppm, from about 600 ppm to about 1000 ppm, from about 700 ppm to about 800 ppm, from about 700 ppm to about 900 ppm, from about 700 ppm to about 1000 ppm, from about 800 ppm to about 900 ppm, about 800 ppm to about 1000 ppm or about 900 ppm to about 1000 ppm.

In another embodiment, the implementation of the composition described in this application does not contain aldehyde.

In one embodiment, the composition described in this application contains one dialdehyde. In another embodiment, the composition described in this application contains many dialdehydes. In aspects according to said embodiment, the composition described herein contains, for example, one or more dialdehydes, two or more dialdehydes, three or more dialdehydes, four or more dialdehydes, or five or more dialdehydes. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, only one dialdehyde, no more than two dialdehydes, no more than three dialdehydes, no more than four dialdehydes, or no more than five dialdehydes. In other aspects, according to the specified implementation variant, the composition described in this application contains, for example, from 1 to 2 dialdehydes, from 1

- 17 042144 up to 3 dialdehydes, 1 to 4 dialdehydes, 1 to 5 dialdehydes, 2 to 3 dialdehydes, 2 to 4 dialdehydes, 2 to 5 dialdehydes, 3 to 4 dialdehydes, 3 to 5 dialdehydes or from 4 to 5 dialdehydes.

You can use any amount described in this application of dialdehyde, provided that this amount is suitable for use in the methods described in this application. In aspects according to the specified embodiment, the composition described in this application contains dialdehyde in an amount of, for example, about 0.01%, about 0.05%, about 0.075%, about 0.1%, about 0.2%, about 0 .3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1.0%, about 1.5 %, about 2.0%, about 2.5%, about 3.0%, about 4.0%, about 5.0%, about 6.0%, about 7.0%, about 7.5%, about 8.0%, about 9.0%, or about 10.0% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains dialdehyde in an amount, for example, at least 0.01%, at least 0.05%, at least 0.075%, at least 0.1% , at least 0.25%, at least 0.5%, at least 0.75%, at least 1.0%, at least 1.5%, at least 2.0%, at least 2.5%, at least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%, at least 7.0%, at least 7.5%, at least 8.0%, at least 9.0% or at least 10.0% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains dialdehyde in an amount, for example, not more than 0.01%, not more than 0.05%, not more than 0.075%, not more than 0.1%, not more than 0 .25%, not more than 0.5%, not more than 0.75%, not more than 1.0%, not more than 1.5%, not more than 2.0%, not more than 2.5%, not more than 3, 0%, 4.0% or less, 5.0% or less, 6.0% or less, 7.5% or less, 8.0% or less, 9.0% or less, or 10.0 or less % by weight of the composition.

In other aspects, according to the specified implementation variant, the composition described in this application contains dialdehyde in an amount, for example, from about 0.1% to about 0.5%, from about 0.1% to about 0.75%, from about 0 1% to about 1.0%, from about 0.1% to about 1.5%, from about 0.1% to about 2.0%, from about 0.1% to about 2.5%, from about 0.2% to about 0.5%, about 0.2% to about 0.75%, about 0.2% to about 1.0%, about 0.2% to about 1.5% , from about 0.2% to about 2.0%, from about 0.2% to about 2.5%, from about 0.5% to about 1.0%, from about 0.5% to about 1, 5%, about 0.5% to about 2.0%, about 0.5% to about 2.5%, about 0.5% to about 3.0%, about 0.5% to about 4.0%, from about 0.5% to about 5.0%, from about 1.0% to about 2.5%, from about 1.0% to about 3.0%, from about 1.0% to about 4.0%, from about 1.0% to about 5.0%, from about 1.0% to about 6.0%, from about 1, 0% to about 7.0%, from about 1.0% to about 7.5%, from about 1.0% to about 8.0%, from about 1.0% to about 9.0%, from about 1.0% to about 10.0%, about 2.0% to about 2.5%, about 2.0% to about 3.0%, about 2.0% to about 4.0%, from about 2.0% to about 5.0%, from about 2.0% to about 6.0%, from about 2.0% to about 7.0%, from about 2.0% to about 7.5 %, from about 2.0% to about 8.0%, from about 2.0% to about 9.0%, from about 2.0% to about 10.0%, from about 5.0% to about 6 .0%, from about 5.0% to about 7.0%, from about 5.0% to about 7.5%, from about 5.0% to about 8.0%, from about 5.0% to about 9.0%, from about 5.0% to about 10.0%, from about 5.0% to about 11.0%, from about 5.0% to about 12.0%, from about 5.0 % to about 13.0%, from about 5.0% to about 14.0%, or about 5.0% to about 15.0% by weight of the composition.

In aspects according to the specified embodiment, the composition described in this application contains dialdehyde in an amount of, for example, 100 ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm, 350ppm, 375ppm, 400ppm, 425ppm, 450ppm, 475ppm, 500ppm, 525ppm, 550ppm, 575ppm, 600ppm, 625ppm, 650ppm, 675ppm, 700ppm, 725ppm, 750ppm , 775ppm, 800ppm, 825ppm, 850ppm, 875ppm, 900ppm, 925ppm, 950ppm, 975ppm or 1000ppm. In other aspects, according to the specified embodiment, the composition described in this application contains dialdehyde in an amount, for example, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least 200 ppm , at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least 625 ppm, at least 650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm , at least 850 ppm, at least 875 ppm, at least 900 ppm, at least 925 ppm, alternate At least 950 ppm, at least 975 ppm, or at least 1000 ppm. In other aspects, according to the specified embodiment, the composition described in this application contains dialdehyde in an amount, for example, not more than 100 ppm, not more than 125 ppm, not more than 150 ppm, not more than 175 ppm, not more than 200 ppm, not more than 225 ppm , no more than 250 ppm, no more than 275 ppm, no more than 300 ppm, no more than 325 ppm, no more than 350 ppm, no more than 375 ppm, no more than 400 ppm, no more

- 18 042144 more 425 ppm, max 450 ppm, max 475 ppm, max 500 ppm, max 525 ppm, max 550 ppm, max 575 ppm, max 600 ppm, max 625 ppm, max 650 675ppm max, 700ppm max, 725ppm max, 750ppm max, 775ppm max, 800ppm max, 825ppm max, 850ppm max, 875ppm max, 900ppm max ppm, 925 ppm or less, 950 ppm or less, 975 ppm or less, or 1000 ppm or less.

In other aspects, according to the specified embodiment, the composition described in this application contains dialdehyde in an amount, for example, from about 100 ppm to about 200 ppm, from about 100 ppm to about 300 ppm, from about 100 ppm to about 400 ppm, from about 100 ppm to about 500 ppm, about 100 ppm to about 600 ppm, about 100 ppm to about 700 ppm, about 100 ppm to about 800 ppm, about 100 ppm to about 900 ppm, about 100 ppm to about 1000 ppm, from about 200 ppm to about 300 ppm, from about 200 ppm to about 400 ppm, from about 200 ppm to about 500 ppm, from about 200 ppm to about 600 ppm, from about 200 ppm to about 700 ppm, from about 200 ppm to about 800 ppm, from about 200 ppm to about 900 ppm, from about 200 ppm to about 1000 ppm, from about 300 ppm to about 400 ppm, from about 300 ppm to about 500 ppm, from about 300 ppm to about 600 ppm , from about 300 ppm to about 70 0 ppm, about 300 ppm to about 800 ppm, about 300 ppm to about 900 ppm, about 300 ppm to about 1000 ppm, about 400 ppm to about 500 ppm, about 400 ppm to about 600 ppm, from about 400 ppm to about 700 ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900 ppm, about 400 ppm to about 1000 ppm, about 500 ppm to about 600 ppm, about 500 ppm to about 700 ppm, from about 500 ppm to about 800 ppm, from about 500 ppm to about 900 ppm, from about 500 ppm to about 1000 ppm, from about 600 ppm to about 700 ppm, from about 600 ppm to about 800 ppm, from about 600 ppm to about 900 ppm, from about 600 ppm to about 1000 ppm, from about 700 ppm to about 800 ppm, from about 700 ppm to about 900 ppm, from about 700 ppm to about 1000 ppm, from about 800 ppm to about 900 ppm , from about 800 ppm to about 1000 ppm, or from about 900 ppm to about 1000 ppm.

In another embodiment, the implementation of the composition described in the invention does not contain dialdehyde.

The composition described in this application may contain an organic peroxide. An organic peroxide is an organic compound containing a peroxide (ROOR') functional group. If one of the R groups is hydrogen, the compound is called an organic hydroperoxide. In one embodiment, the organic peroxide described herein is a compound of Formula VII, wherein R ³ and R ⁴ can each independently be H, C _1-6 alkyl, C _1-6 alkane, C _1-6 alkyne , C _3-6 cyclic alkyl or C _3-6 aryl.

I.O. ^.R4

R ³ O VII

Non-limiting examples of organic peroxides include benzoyl peroxide and peroxyacetic acid (peracetic acid).

In one embodiment, the composition described in this application contains one organic peroxide. In another embodiment, the composition described in this application contains many organic peroxides. In aspects according to said embodiment, the composition described herein comprises, for example, one or more organic peroxides, two or more organic peroxides, three or more organic peroxides, four or more organic peroxides, or five or more organic peroxides. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, only one organic peroxide, no more than two organic peroxides, no more than three organic peroxides, no more than four organic peroxides, or no more than five organic peroxides. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, from 1 to 2 organic peroxides, from 1 to 3 organic peroxides, from 1 to 4 organic peroxides, from 1 to 5 organic peroxides, from 2 to 3 organic peroxides, 2 to 4 organic peroxides, 2 to 5 organic peroxides, 3 to 4 organic peroxides, 3 to 5 organic peroxides, or 4 to 5 organic peroxides.

Any amount of the organic peroxide described herein may be used, provided that the amount is suitable for use in the methods described herein. In aspects according to the specified implementation variant, the composition described in this application contains organic peroxide in an amount of, for example, about 0.01%, about 0.05%, about 0.075%, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1.0%, about 1, 5%, about 2.0%, about 2.5%, about 3.0%, about 4.0%, about 5.0%, about 6.0%, about 7.0%, about 7.5% , about 8.0%, about 9.0%, or about 10.0% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains an organic peroxide in an amount, for example, at least 0.01%, at least 0.05%, at least 0.075%, at least 0.1 %, at least 0.25%, at least 0.5%, at least

at least 0.75%, at least 1.0%, at least 1.5%, at least 2.0%, at least 2.5%, at least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%, at least 7.0%, at least 7.5%, at least 8.0%, at least at least 9.0% or at least 10.0% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains organic peroxide in an amount, for example, not more than 0.01%, not more than 0.05%, not more than 0.075%, not more than 0.1%, not more than 0.25%, max 0.5%, max 0.75%, max 1.0%, max 1.5%, max 2.0%, max 2.5%, max 3 .0%, not more than 4.0%, not more than 5.0%, not more than 6.0%, not more than 7.5%, not more than 8.0%, not more than 9.0% or not more than 10, 0% by weight of the composition.

In other aspects, according to the specified embodiment, the composition described in this application contains organic peroxide in an amount, for example, from about 0.1% to about 0.5%, from about 0.1% to about 0.75%, from about 0.1% to about 1.0%, about 0.1% to about 1.5%, about 0.1% to about 2.0%, about 0.1% to about 2.5%, about 0.2% to about 0.5%, about 0.2% to about 0.75%, about 0.2% to about 1.0%, about 0.2% to about 1.5 %, from about 0.2% to about 2.0%, from about 0.2% to about 2.5%, from about 0.5% to about 1.0%, from about 0.5% to about 1 .5%, from about 0.5% to about 2.0%, from about 0.5% to about 2.5%, from about 0.5% to about 3.0%, from about 0.5% to about 4.0%, from about 0.5% to about 5.0%, from about 1.0% to about 2.5%, from about 1.0% to about 3.0%, from about 1.0 % to about 4.0%, from about 1.0% to about 5.0%, from about 1.0% to about 6.0%, from about 1.0% to about 7.0%, from about 1.0% to about 7.5%, from about 1.0% to about 8.0%, from about 1.0% to about 9.0% , from about 1.0% to about 10.0%, from about 2.0% to about 2.5%, from about 2.0% to about 3.0%, from about 2.0% to about 4, 0%, about 2.0% to about 5.0%, about 2.0% to about 6.0%, about 2.0% to about 7.0%, about 2.0% to about 7.5%, from about 2.0% to about 8.0%, from about 2.0% to about 9.0%, from about 2.0% to about 10.0%, from about 5.0% up to about 6.0%, from about 5.0% to about 7.0%, from about 5.0% to about 7.5%, from about 5.0% to about 8.0%, from about 5, 0% to about 9.0%, about 5.0% to about 10.0%, about 5.0% to about 11.0%, about 5.0% to about 12.0%, from about 5.0% to about 13.0%, from about 5.0% to about 14.0%, or from about 5.0% to about 15.0% by weight of the composition.

In aspects according to the specified embodiment, the composition described in this application contains organic peroxide in an amount of, for example, 100 ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm . ppm, 775 ppm, 800 ppm, 825 ppm, 850 ppm, 875 ppm, 900 ppm, 925 ppm, 950 ppm, 975 ppm or 1000 ppm. In other aspects, according to the specified implementation variant, the composition described in this application contains an organic peroxide in an amount, for example, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least 200 ppm, at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least at least 625 ppm, at least 650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm at least 850 ppm at least 875 ppm at least 900 ppm at least 925 ppm, at least 950 ppm, at least 975 ppm, or at least 1000 ppm. In other aspects, according to the specified implementation variant, the composition described in this application contains organic peroxide in an amount, for example, not more than 100 ppm, not more than 125 ppm, not more than 150 ppm, not more than 175 ppm, not more than 200 ppm, not more than 225 250ppm max, 275ppm max, 300ppm max, 325ppm max, 350ppm max, 375ppm max, 400ppm max, 425ppm max, 450ppm max, 475ppm max 500ppm max, 525ppm max, 550ppm max, 575ppm max, 600ppm max, 625ppm max, 650ppm max, 675ppm max, 700ppm max, 725ppm max 750ppm max, 775ppm max, 800ppm max, 825ppm max, 850ppm max, 875ppm max, 900ppm max, 925ppm max, 950ppm max, 975ppm max ppm or not more than 1000 ppm.

In other aspects, according to the specified embodiment, the composition described in this application contains organic peroxide in an amount, for example, from about 100 ppm to about 200 ppm, from about 100 ppm to about 300 ppm, from about 100 ppm to about 400 ppm, from about 100 ppm to about 500 ppm, about 100 ppm to about 600 ppm, about 100 ppm to about 700 ppm, about 100 ppm to about 800 ppm, about 100 ppm to about 900 ppm, about 100 ppm to about 1000 ppm, about 200 ppm to about 300 ppm, about 200 ppm to about 400 ppm, about 200 ppm to about 500 ppm, from about 200

- 20 042144 ppm to about 600 ppm, from about 200 ppm to about 700 ppm, from about 200 ppm to about 800 ppm, from about 200 ppm to about 900 ppm, from about 200 ppm to about 1000 ppm, from about 300 ppm to about 400 ppm, from about 300 ppm to about 500 ppm, from about 300 ppm to about 600 ppm, from about 300 ppm to about 700 ppm, from about 300 ppm to about 800 ppm, from about 300 ppm to about 900 ppm, from about 300 ppm to about 1000 ppm, about 400 ppm to about 500 ppm, about 400 ppm to about 600 ppm, about 400 ppm to about 700 ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900 ppm, about 400 ppm to about 1000 ppm, about 500 ppm to about 600 ppm, about 500 ppm to about 700 ppm, about 500 ppm to about 800 ppm, about 500 ppm to about 900 ppm, from about 500 ppm to about 1000 ppm, from about 600 ppm to about 700 ppm, from about 600 ppm to about 800 ppm, from about 600 ppm to about 900 ppm, from about 600 ppm to about 1000 ppm, from about 700 ppm to about 800 ppm, from about 700 ppm to about 900 ppm, from about 700 ppm to about 1000 ppm , from about 800 ppm to about 900 ppm, from about 800 ppm to about 1000 ppm, or from about 900 ppm to about 10θΟ ppm.

In another embodiment, the implementation of the composition described in this application does not contain organic peroxide.

The composition described in this application may contain one or more alcohols. An organic peroxide is an organic compound containing a peroxide (ROOR') functional group. If one of the R groups is hydrogen, the compound is called an organic hydroperoxide. In one embodiment, the organic peroxide described herein is a compound of Formula VII, wherein R ³ and R ⁴ can each independently be H, C _1-6 alkyl, C _1-6 alkane, C 1-6 alkyne, _C3-6 cyclic alkyl or _C3-6 aryl.

An alcohol is an organic molecule containing a hydroxyl functional group (-OH) bonded to a carbon atom, where the carbon atom is saturated. In aspects according to said embodiment, the alcohol may be, for example, C _2-4 alcohol, C _1-4 alcohol, Ci. ₅ alcohol, C _1-7 alcohol, _C1-10 alcohol, C1_ ₁₅ alcohol, or _C1-20 alcohol. In other aspects, according to the specified embodiment, the alcohol may be, for example, a primary alcohol, a secondary alcohol, or a tertiary alcohol. In other aspects, according to said embodiment, the alcohol may be, for example, an acyclic alcohol, a monohydric alcohol, a polyhydric alcohol (also known as a polyol or sugar alcohol), an unsaturated aliphatic alcohol, an alicyclic alcohol, or a combination thereof. Examples of monohydric alcohols include, without limitation, methanol, ethanol, propanol, isopropanol, butanol, pentanol, and 1-hexadecanol. Examples of polyhydric alcohols include, without limitation, glycol, glycerol, arabitol, erythritol, xylitol, maltitol, sorbitol (glucite), mannitol, inositol, lactitol, galactitol (idit) and isomalt. Examples of unsaturated aliphatic alcohols include, without limitation, prop-2-en-1-ol, 3,7-dimethylocta-2,6-dien-1-ol, and prop-2-yn-1-ol. Examples of alicyclic alcohols include, without limitation, cyclohexane-1,2,3,4,5,6-hexol and 2-(2-propyl)-5-methyl-cyclohexan1-one.

In one embodiment, the composition described in this application contains one alcohol. In another embodiment, the composition described in this application contains many alcohols. In aspects according to said embodiment, the composition described herein comprises, for example, one or more alcohols, two or more alcohols, three or more alcohols, four or more alcohols, or five or more alcohols. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, only one alcohol, no more than two alcohols, no more than three alcohols, no more than four alcohols, or no more than five alcohols. In other aspects according to the specified embodiment, the composition described in this application contains, for example, from 1 to 2 alcohols, from 1 to 3 alcohols, from 1 to 4 alcohols, from 1 to 5 alcohols, from 2 to 3 alcohols, from 2 up to 4 alcohols, 2 to 5 alcohols, 3 to 4 alcohols, 3 to 5 alcohols, or 4 to 5 alcohols.

In aspects of this embodiment, the plurality of alcohols contained in a composition described herein may be, for example, about 95% first alcohol and about 5% second alcohol, about 90% first alcohol and about 10% second alcohol, about 80% about 70% first alcohol and about 30% second alcohol, about 66.7% first alcohol and about 33.3% second alcohol, about 60% first alcohol and about 40% second alcohol, about 50% first alcohol and about 50% second alcohol, about 40% first alcohol and about 60% second alcohol, about 30% first alcohol and about 70% second alcohol, about 20% first alcohol and about 80% second alcohol, about 10% first alcohol and about 90% second alcohol, about 5% first alcohol and about 95% second alcohol. In other aspects, according to the specified embodiment, the ratio of the first alcohol to the second alcohol is, for example, 100:1, 90:1, 80:1, 70:1, 60:1, 50:1, 40:1, 30:1, 20 :1, 19:1, 18:1, 17:1, 16:1, 15:1, 14:1, 13:1, 12:1, 11:1, 10:1, 9:1, 8:1 , 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1 :7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19 , 1:20, 1:30, 1:40, 1:50, 1:60, 1:70, 1:80, 1:90 or 1:100. In other aspects, the first alcohol is isopropanol and the second alcohol is

- 21 042144 is ethanol.

You can use any amount of alcohol described in this application, provided that this amount is suitable for use in the methods described in this application. In aspects according to the specified implementation variant, the composition described in this application contains alcohol in an amount of, for example, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40 %, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 96%, about 97%, about 98%, or about 99% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains alcohol in an amount, for example, at least 5%, at least 10%, at least 15%, at least 20%, at least 25% at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% by weight of the composition. In other aspects, according to the specified embodiment, the composition described in this application contains alcohol in an amount, for example, not more than 5%, not more than 10%, not more than 15%, not more than 20%, not more than 25%, not more than 30% , no more than 35%, no more than 40%, no more than 45%, no more than 50%, no more than 55%, no more than 60%, no more than 65%, no more than 70%, no more than 75%, no more than 80% , not more than 85%, not more than 90%, not more than 95%, not more than 96%, not more than 97%, not more than 98% or not more than 99% by weight of the composition. In other aspects, according to the specified embodiment, the composition described in this application contains alcohol in an amount, for example, from about 5% to about 10%, from about 5% to about 20%, from about 5% to about 30%, from about 5% to about 40%, about 5% to about 50%, about 5% to about 60%, about 5% to about 70%, about 5% to about 80%, about 5% to about 90 %, from about 5% to about 95%, from about 5% to about 97%, from about 5% to about 99%, from about 10% to about 20%, from about 10% to about 30%, from about 10 % to about 40%, from about 10% to about 50%, from about 10% to about 60%, from about 10% to about 70%, from about 10% to about 80%, from about 10% to about 90% , from about 10% to about 95%, from about 10% to about 97%, from about 10% to about 99%, from about 20% to about 30%, from about 20% to about 40%, from about 20% up to about 50%, from about 20% up to about 60%, from about 20% to about 70%, from about 20% to about 80%, from about 20% to about 90%, from about 20% to about 95%, from about 20% to about 97%, from about 20% to about 99%, from about 30% to about 40%, from about 30% to about 50%, from about 30% to about 60%, from about 30% to about 70%, from about 30% to about 80%, from about 30% to about 90%, from about 30% to about 95%, from about 30% to about 97%, from about 30% to about 99%, from about 35% to about 40%, from about 35% to about 50%, about 35% to about 60%, about 35% to about 70%, about 35% to about 80%, about 35% to about 90%, about 35% to about 95%, about 35% to about 97%, about 35% to about 99%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, from about 40% to about 80%, about 40% to about 90%, about 40% to but 95%, from about 40% to about 97%, from about 40% to about 99%, from about 45% to about 50%, from about 45% to about 60%, from about 45% to about 70%, from about 45% to about 80%, about 45% to about 90%, about 45% to about 95%, about 45% to about 97%, about 45% to about 99%, about 50% to about 60%, from about 50% to about 70%, from about 50% to about 80%, from about 50% to about 90%, from about 50% to about 95%, from about 50% to about 97%, from about 50% to about 99%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 60% to about 97 %, from about 60% to about 99%, from about 70% to about 80%, from about 70% to about 90%, from about 70% to about 95%, from about 70% to about 97%, from about 70 % to about 99%, from about 80% to about 90%, from about 80% to about 95%, from about 80% to about 97%, about 80% to about 99%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99% by weight of the composition.

In aspects according to the specified embodiment, the composition described in this application contains a plurality of alcohols in a total amount, for example, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 96 %, about 97%, about 98% or about 99% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains a plurality of alcohols in a total amount, for example, at least 5%, at least 10%, at least 15%, at least 20%, at least

- 22 042144 re 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% by weight of the composition. In other aspects, according to the specified embodiment, the composition described in this application contains a plurality of alcohols in a total amount, for example, no more than 5%, no more than 10%, no more than 15%, no more than 20%, no more than 25%, no more 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, max 80%, not more than 85%, not more than 90%, not more than 95%, not more than 96%, not more than 97%, not more than 98% or not more than 99% by weight of the composition. In other aspects, according to the specified embodiment, the composition described in this application contains a plurality of alcohols in a total amount, for example, from about 5% to about 10%, from about 5% to about 20%, from about 5% to about 30%, from about 5% to about 40%, from about 5% to about 50%, from about 5% to about 60%, from about 5% to about 70%, from about 5% to about 80%, from about 5% to about 90%, from about 5% to about 95%, from about 5% to about 97%, from about 5% to about 99%, from about 10% to about 20%, from about 10% to about 30%, from about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 10% to about 97%, about 10% to about 99%, about 20% to about 30%, about 20% to about 40%, from about 20% to about 50 %, from about 20% to about 60%, from about 20% to about 70%, from about 20% to about 80%, from about 20% to about 90%, from about 20% to about 95%, from about 20 % to about 97%, from about 20% to about 99%, from about 30% to about 40%, from about 30% to about 50%, from about 30% to about 60%, from about 30% to about 70% , from about 30% to about 80%, from about 30% to about 90%, from about 30% to about 95%, from about 30% to about 97%, from about 30% to about 99%, from about 35% up to about 40%, from about 35% to about 50%, from about 35% to about 60%, from about 35% to about 70%, from about 35% to about 80%, from about 35% to about 90%, from about 35% to about 95%, from about 35% to about 97%, from about 35% to about 99%, from about 40% to about 50%, from about 40% to about 60%, from about 40% to about 70%, from about 40% to about 80%, from about 40% to about 90%, from approx. 40% to about 95%, about 40% to about 97%, about 40% to about 99%, about 45% to about 50%, about 45% to about 60%, about 45% to about 70%, about 45% to about 80%, about 45% to about 90%, about 45% to about 95%, about 45% to about 97%, about 45% to about 99%, from about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 50% to about 97 %, from about 50% to about 99%, from about 60% to about 70%, from about 60% to about 80%, from about 60% to about 90%, from about 60% to about 95%, from about 60 % to about 97%, from about 60% to about 99%, from about 70% to about 80%, from about 70% to about 90%, from about 70% to about 95%, from about 70% to about 97% , from about 70% to about 99%, from about 80% to about 90%, from about 80% to about 95%, about 80% to about 97%, about 80% to about 99%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99% of weight of the composition.

In another embodiment, the implementation of the composition described in this application does not contain alcohol. In one aspect, according to the specified implementation variant, the composition described in this application does not contain ethanol. In another aspect, according to the specified implementation variant, the composition described in this application does not contain isopropanol.

The composition described in this application may contain a carrier. The carrier, also known as the carrier substance, can be any material commonly known in the field of skin care, cosmetics and medicine, which is used as a basis for obtaining the composition described in this application. The carrier may be an aqueous carrier, a semi-solid carrier or a solid carrier. The carrier may also provide skin care, as described in this application. The carrier includes, without limitation, water, vegetable oil, mineral oil, essential oil, ether, alcohol, fatty alcohol, isoparaffin, hydrocarbon oil, polyhydric alcohol, and wax. Non-limiting examples of essential oils include octyl palmitate, isopropyl myristate, and isopropyl palmitate. Non-limiting examples of ethers include dicapryl ether and dimethyl isosorbide. Non-limiting examples of alcohols include ethanol and isopropanol. Non-limiting examples of fatty alcohols include cetyl alcohol, cetearyl alcohol, stearyl alcohol, and behenyl alcohol. Non-limiting examples of isoparaffins include isooctane, isododecane (IDD) and isohexadecane. Non-limiting examples of hydrocarbon oils include mineral oil, petrolatum, isoeicosan, and polyolefin, including (hydrogenated) polyisobutene. unlimited

- 23 042144 Examples of polyhydric alcohols include propylene glycol, glycerin, butylene glycol, pentylene glycol, hexylene glycol, caprylyl glycol. Non-limiting examples of waxes include beeswax, carnauba wax, ozocerite, microcrystalline wax, polyethylene wax, and botanical wax.

In one embodiment, the composition described in this application contains one carrier. In another embodiment, the composition described in this application contains many carriers. In aspects according to the specified embodiment, the composition described in this application contains, for example, one or more carriers, two or more carriers, three or more carriers, four or more carriers, or five or more carriers. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, only one carrier, no more than two carriers, no more than three carriers, no more than four carriers, or no more than five carriers. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, from 1 to 2 carriers, from 1 to 3 carriers, from 1 to 4 carriers, from 1 to 5 carriers, from 2 to 3 carriers, from 2 up to 4 media, 2 to 5 media, 3 to 4 media, 3 to 5 media, or 4 to 5 media.

In another embodiment, the implementation of the composition described in this application contains an amount of carrier that provides the desired prescription or beneficial effect of the composition described in this application. In aspects according to the specified embodiment, the composition described in this application contains a carrier in an amount, for example, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains a carrier in an amount, for example, not more than 25%, not more than 30%, not more than 35%, not more than 40%, not more than 45%, not more than 50% , no more than 55%, no more than 60%, no more than 65%, no more than 70%, no more than 75%, no more than 80%, no more than 85%, no more than 90%, no more than 95%, no more than 96% , not more than 97%, not more than 98% or not more than 99% by weight of the composition. In other aspects, according to the specified embodiment, the composition described in this application contains a carrier in an amount, for example, from about 25% to about 50%, from about 25% to about 75%, from about 25% to about 90%, from about 25% to about 95%, about 25% to about 96%, about 25% to about 97%, about 25% to about 98%, about 25% to about 99%, about 50% to about 75 %, from about 50% to about 90%, from about 50% to about 95%, from about 50% to about 96%, from about 50% to about 97%, from about 50% to about 98%, from about 50 % to about 99%, from about 75% to about 80%, from about 75% to about 85%, from about 75% to about 90%, from about 75% to about 95%, from about 75% to about 96% , from about 75% to about 97%, from about 75% to about 98%, from about 75% to about 99%, from about 80% to about 85%, from about 80% to about 90%, from about 80% up to about 95%, from about 80% to about 96%, from about 80% to about 97%, from about 80% to about 98%, from about 80% to about 99%, from about 85% to about 90%, from about 85% to about 95%, from about 85% to about 96%, from about 85% to about 97%, from about 85% to about 98%, from about 85% to about 99%, from about 90% to about 95%, from about 90% to about 96%, about 90% to about 97%, about 90% to about 98%, about 90% to about 99%, or about 95% to about 99% by weight of the composition.

In another embodiment, the implementation of the composition described in this application contains a carrier other than alcohol. In aspects according to the specified implementation variant, if the composition described in this application contains hypochlorous acid or free active chlorine, then the composition may contain a carrier other than alcohol.

The composition described in this application may additionally optionally include additional ingredients. An additional ingredient is an ingredient useful in obtaining the final composition described in this application. The additional ingredient includes, without limitation, a surfactant, preservative or chelating agent. The additional ingredient described in this application is known in the art.

Surfactants are compounds that reduce the surface tension of a liquid, allowing for easier spreading and reduction of interfacial tension between two liquids or between a liquid and a solid. Acting at the interface between two surfaces, surfactants change the interfacial tension, which leads to the formation of foam. A single surfactant may be mixed with the buffered solution described herein, or a plurality of surfactants may be mixed with the buffered solution described herein. Suitable surfactants for use include, without limitation, ionic surfactants, zwitterionic (amphoteric) surfactants, non-ionic surfactants

- 24 042144 or any combination thereof. The person skilled in the art can vary the surfactant used in the composition described in this application, and it usually depends, in particular, on the specific buffer used, the eluting protein and the conductivity values used.

Ionic surfactants include anionic surfactants. Anionic surfactants include those based on permanent functional groups attached to the head, such as, for example, sulfates, sulfonates, phosphates and carboxylates, or pH dependent anionic surfactants. Anionic surfactants include, without limitation, alkyl sulfates such as ammonium lauryl sulfate and sodium lauryl sulfate (SDS); alkyl ether sulfates such as sodium laureth sulfate and sodium myreth sulfate; docusates such as sodium dioctylsulfosuccinate; fluorosulfonate surfactants such as perfluorooctanesulfonate (PFOS) and perfluorobutanesulfonate; alkyl diphenyl oxide disulfonates such as DOWFAX™ 2A1 (lauryl phenyl disulfonate ether disodium salt), DOWFAX™ 3B2 (decylphenyl disulfonate ether disodium salt), DOWFAX™ C10L (decylphenyl disulfonate ether disodium salt), DOWFAX™ 2EP and DOWFAX™ 8390 (ether ether disodium salt) cetylphenyl disulfonate); potassium salts of phosphate esters such as TRITON™ H-55 and TRITON™ H-66; alkylbenzenesulfonates; alkylaryl ether phosphates; alkyl ether phosphates; alkyl carboxylates such as fatty acid salts and sodium stearate; sodium lauryl sarcosinate; a fluorocarboxylate surfactant such as perfluorononanoate and perfluorooctanoate; and hexyl diphenyl sulfonate ether sodium salt (DOWFAX™ C6L).

Ionic surfactants also include cationic surfactants. Cationic surfactants include those based on permanent or pH dependent cationic surfactants such as, for example, primary, secondary or tertiary amines. Cationic surfactants include, without limitation, quaternary ammonium surfactants such as alkyltrimethylammonium salts and alkylbenzyldimethylammonium salts, as well as pH dependent primary, secondary or tertiary amines, such as surfactants in which the primary amines become positively charged at pH greater than 10, or secondary amines become charged at pH less than 4, such as octenidine dihydrochloride. Non-limiting examples of quaternary ammonium surfactants include first generation quaternary ammonium surfactants containing C ₁₂ _ ₁₈ alkyl chains, second generation quaternary ammonium surfactants containing aromatic ring groups, surfactants third generation quaternary ammonium compounds containing first generation quaternary ammonium double compounds and/or alkyldimethylammonium chloride mixtures, fourth generation quaternary ammonium surfactants containing double chain dialkylmethylamines, fifth generation quaternary ammonium surfactants containing synergistic combinations of double quaternary ammonium compounds, sixth generation quaternary ammonium surfactants containing polymeric quaternary ammonium compounds, and surfactants based on a seventh generation quaternary ammonium containing bis-quaternary ammonium compounds with polymeric quaternary ammonium compounds. Quaternary ammonium surfactants include, without limitation, benzalkonium chloride (BAC), benzethonium chloride (BZT), benzododecinium bromide (or dimethyldodecylbenzylammonium bromide), bronidox (or 5-bromo-5nitro-1,3-dioxane), carbetopendecinium bromide, cetalkonium chloride (CKC), cetrimonium, cetrimide, cetylpyridinium chloride (CPC), cetyltrimethylammonium bromide (CTAB or cetrimonium bromide) and cetyltrimethylammonium chloride (CTAC or cetrimonium chloride), didecyldimethylammonium chloride (DDAC), dioctadecyldimethylammonium bromide (DODAB or dioctadecylammonium) , dioctadecyldimethylammonium chloride (DODAC or dimethyldioctadecylammonium chloride), docosyltrimethylammonium chloride (DCTAC or behentrimonium chloride), dophanium chloride, domiphen bromide, methylbenzetonium chloride, octenidine dihydrochloride, polydronium chloride, stearylalkonium chloride (or dimethylbenzyloctadecylammonium), tetraethylammonium, and tetramethylammonium bromide tonsonium bromide. Other suitable anionic surfactants for use include biobased anionic surfactants, including, without limitation, STEPONOL® AM 30-KE, ammonium lauryl sulfate and STEPONOL® EHS, sodium 2-ethylhexyl sulfate. Such bio-based surfactants are not synthetic molecules and are anionic bio-based surfactants derived from organic raw materials such as plants.

Zwitterionic surfactants are based on primary, secondary or tertiary amines or quaternary ammonium cations with a sulfonate, carboxylate or phosphate. Zwitterionic surfactants include, without limitation, 3-[(3-cholamidopropyl)dimethyl

- 25 042144 ammonio]-1-propanesulfonate (CHAPS); sultaines such as cocamidopropylhydroxysultaine; betaines such as cocamidopropyl betaine; or lecithins.

Nonionic surfactants are less denaturing and as such are suitable for solubilizing membrane proteins and lipids while maintaining protein-protein interactions. Nonionic surfactants include polyether nonionic surfactants, polyhydroxyl nonionic surfactants and biobased surfactants. Nonionic surfactants include alcohol ethoxylates, alkylphenol ethoxylates, phenol ethoxylates, amide ethoxylates, glyceride ethoxylates, fatty acid ethoxylates, fatty amine ethoxylates, and polyethoxylated tallow amine (POEA). Other suitable nonionic surfactants include biobased nonionic surfactants, including but not limited to STEPOSOL® MET-10U, a metathesis nonionic surfactant that is a short chain unsaturated amide. Such bio-based surfactants are non-synthetic molecules and are non-ionic bio-based surfactants derived from organic raw materials such as plants.

Non-limiting examples of surfactants include polyoxyethylene glycol sorbitan alkyl esters (or ethoxylated sorbitan esters) such as polysorbate 20 sorbitan monooleate (TWEEN® 20), polysorbate 40 sorbitan monooleate (TWEEN® 40), polysorbate 60 sorbitan monooleate (TWEEN® 60), polysorbate 61 sorbitan monooleate (TWEEN® 61), polysorbate 65 sorbitan monooleate (TWEEN® 65), polysorbate 80 sorbitan monooleate (TWEEN® 80), polysorbate 81 sorbitan monooleate (TWEEN® 81) and polysorbate 85 sorbitan monooleate (TWEEN® 85); esters of sorbitan such as sorbitan monooleate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate and sorbitan tristearate; polyglycerol esters such as glycerol monooleate, glycerol monolaurate, glycerol monopalmitate, glycerol monostearate, glycerol trioleate, glycerol ricinoleate, glycerol tristearate, glycerol monodiglycerides, and glycerol triacetate; ethoxylated polyglycerol esters; alkyl glucosides such as arachidyl glucoside, C _12-20 alkyl glucoside, caprylyl/capryl glucoside _, cetearyl glucoside, coco glucoside, ethyl glucoside and lauryl glucoside, decyl glucoside; ethoxylated alkyl glucosides; sucrose esters such as sucrose monooleate, sucrose monolaurate, sucrose monopalmitate, sucrose monostearate, sucrose trioleate, sucrose ricinoleate, sucrose tristearate, sucrose diglycerides and sucrose triacetate; ethoxylated sucrose ester; amine oxides; ethoxylated alcohols; ethoxylated aliphatic alcohols; alkylamines; ethoxylated alkylamines; ethoxylated alkylphenols such as ethoxylated nonylphenol and ethoxylated octylphenol; alkylpolysaccharides; ethoxylated alkyl polysaccharides; ethoxylated fatty acids such as ethoxylated castor oil; ethoxylated fatty alcohols such as ethoxylated cetoolyl alcohol, ethoxylated cetostearyl alcohol, ethoxylated decyl alcohol, ethoxylated dodecyl alcohol, and ethoxylated tridecyl alcohol; ethoxylated fatty amines; poloxamers (copolymers of polyethylene and polypropylene) such as poloxamer 124 (PLURONIC® L44), poloxamer 181 (PLURONIC® L61), poloxamer 182 (PLURONIC® L62), poloxamer 184 (PLURONIC® L64), poloxamer 188 (PLURONIC® F68), poloxamer 237 (PLURONIC® F87), poloxamer 338 (PLURONIC® L108) and poloxamer 407 (PLURONIC® F127); linear secondary alcohol ethoxylates such as TERGITOL™ 15-S-5, TERGITOL™ 15-S-7, TERGITOL™ 15-S-9, TERGITOL™ 15-S-12, TERGITOL™ 15-S-15, TERGITOL™ 15 -S-20, TERGITOL™ 15-S-30 and TERGITOL™ 15-S-40; simple alkylphenolpolyglycol ethers; simple alkylaryl ethers of polyethylene glycol; polyoxyethylene glycol alkyl ethers such as octaethylene glycol monododecyl ether, pentaethylene glycol monododecyl ether, BRIJ® 30 and BRIJ®35; 2-dodecyloxyethanol (LUBROL®-PX); polyoxyethyl glycol octylphenol ethers such as polyoxyethylene (4-5) p-tert-octylphenol (TRITON® X45) and polyoxyethylene octylphenyl ether (TRITON® X-100); alkyl polyglucoside (TRITON® CG-110); polyoxyethylene glycol alkylphenol ethers such as nonoxynol-9; phenoxypolyethoxyethanols such as nonylphenoxypolyethoxyethanol and octylphenoxypolyethoxyethanol (IGEPAL® CA-630 or NONIDET™ P-40); glucoside alkyl ethers such as octylglucopyranoside; maltoside alkyl ethers such as dodecylmaltopyranoside; thioglucoside alkyl ethers such as heptylthioglucopyranoside; digitonins; glycerol alkyl esters such as glyceryl laurate; alkylarylpolyether sulfates; alcohol sulfonates; alkyl esters of sorbitan; cocamideethanolamines such as cocamidemonoethanolamine and cocamidediethanolamine; sucrose monolaurate; dodecyldimethylamine oxide and sodium cholate. Other non-limiting examples of surfactants suitable for use in the methods described in this application can be found, for example, in Winslow et al., Methods and Compositions for Simultaneously Isolating Hemoglobin from Red Blood Cells and Inactivating Viruses, US 2008/0138790; Pharmaceutical Dosage Forms and Drug Delivery Systems (Howard C. Ansel et al, eds., Lippincott Williams &

- 26 042144

Wilkins Publishers, ^7th ed. 1999); Remington: The Science and Practice of Pharmacy (Alfonso R. Gennaro ed., Lippincott, Williams & Wilkins, ^20th ed. 2000); Goodman &Gilman's The Pharmacological Basis of Therapeutics (Joel G. Hardman et al., eds., McGraw-Hill Professional, ^10th ed. 2001); and Handbook of Pharmaceutical Excipients (Raymond C. Rowe et al., APhA Publications, ^4th edition 2003), the contents of each of which are incorporated herein in their entirety by reference.

In one embodiment, the composition described herein contains a single surfactant. In another embodiment, the composition described in this application contains a variety of surfactants. In aspects according to said embodiment, the composition described herein comprises, for example, one or more surfactants, two or more surfactants, three or more surfactants, four or more surfactants, or five or more surfactants. In other aspects, according to the specified embodiment, the composition described in this application contains, for example, only one surfactant, no more than two surfactants, no more than three surfactants, no more than four surfactants, or no more than five surfactants. In other aspects, according to the specified implementation variant, the composition described in this application contains, for example, from 1 to 2 surfactants, from 1 to 3 surfactants, from 1 to 4 surfactants, from 1 to 5 surfactants -active agents, 2 to 3 surfactants, 2 to 4 surfactants, 2 to 5 surfactants, 3 to 4 surfactants, 3 to 5 surfactants, or 4 to 5 surfactants.

Any amount of the surfactant described herein may be used, provided that the amount is suitable for the application of the methods described herein. In aspects according to the specified embodiment, the composition described in this application contains a surfactant in an amount of, for example, about 0.001%, about 0.005%, about 0.0075%, about 0.01%, about 0.05%, about 0.075%, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8% , about 0.9%, about 1.0%, about 1.5%, about 2.0%, about 2.5%, about 3.0%, about 4.0%, about 5.0%, about 6.0%, about 7.0%, about 7.5%, about 8.0%, about 9.0% or about 10.0% by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains a surfactant in an amount, for example, at least 0.001%, at least 0.005%, at least 0.0075%, at least 0.01 %, at least 0.05%, at least 0.075%, at least 0.1%, at least 0.25%, at least 0.5%, at least 0.75%, at least at least 1.0%, at least 1.5%, at least 2.0%, at least 2.5%, at least 3.0%, at least 4.0%, at least 5 .0%, at least 6.0%, at least 7.0%, at least 7.5%, at least 8.0%, at least 9.0%, or at least 10.0 % by weight of the composition. In other aspects, according to the specified implementation variant, the composition described in this application contains a surfactant in an amount, for example, not more than 0.001%, not more than 0.005%, not more than 0.0075%, not more than 0.01%, not more 0.05%, max 0.075%, max 0.1%, max 0.25%, max 0.5%, max 0.75%, max 1.0%, max 1.5 %, max 2.0%, max 2.5%, max 3.0%, max 4.0%, max 5.0%, max 6.0%, max 7.5% , not more than 8.0%, not more than 9.0% or not more than 10.0% by weight of the composition.

In other aspects, according to the specified embodiment, the composition described in this application contains a surfactant in an amount, for example, from about 0.001% to about 0.005%, from about 0.001% to about 0.0075%, from about 0.001% to about 0.01%, from about 0.001% to about 0.025%, from about 0.001% to about 0.05%, from about 0.001% to about 0.075%, from about 0.001% to about 0.1%, from about 0.001% to about 0.25%, from about 0.001% to about 0.75%, from about 0.001% to about 1.0%, from about 0.005% to about 0.0075%, from about 0.005% to about 0.01%, from about 0.005% to about 0.025%, from about 0.005% to about 0.05%, from about 0.005% to about 0.075%, from about 0.005% to about 0.1%, from about 0.005% to about 0.25% , from about 0.005% to about 0.75%, from about 0.005% to about 1.0%, from about 0.01% to about 0.05%, from about 0.01% to about 0.075%, from about 0 .01% to about 0.1% , from about 0.01% to about 0.25%, from about 0.01% to about 0.5%, from about 0.01% to about 0.75%, from about 0.01% to about 1, 0%, about 0.01% to about 1.5%, about 0.01% to about 2.0%, about 0.01% to about 2.5%, about 0.05% to about 0.075%, about 0.05% to about 0.1%, about 0.05% to about 0.25%, about 0.05% to about 0.5%, about 0.05% to about 0.75%, from about 0.05% to about 1.0%, from about 0.05% to about 1.5%, from about 0.05% to about 2.0%, from about 0.05% to about 2.5%, from about 0.1% to about 0.5%, from about 0.1% to about 0.75%, from about 0.1% to about 1.0%, from about 0, 1% to about 1.5%, from about 0.1% to about 2.0%, from about 0.1% to about 2.5%, from about 0.1% to

- 27 042144 about 3.0%, from about 0.1% to about 4.0%, from about 0.1% to about 5.0%, from about 0.2% to about 0.5%, from about 0.2% to about 0.75%, about 0.2% to about 1.0%, about 0.2% to about 1.5%, about 0.2% to about 2.0%, about 0.2% to about 2.5%, about 0.2% to about 3.0%, about 0.2% to about 4.0%, about 0.2% to about 5.0 %, from about 0.5% to about 1.0%, from about 0.5% to about 1.5%, from about 0.5% to about 2.0%, from about 0.5% to about 2 .5%, from about 0.5% to about 3.0%, from about 0.5% to about 4.0%, from about 0.5% to about 5.0%, from about 0.5% to about 6.0%, from about 0.5% to about 7.0%, from about 0.5% to about 8.0%, from about 0.5% to about 9.0%, from about 0.5 % to about 10.0%, from about 1.0% to about 2.5%, from about 1.0% to about 3.0%, from about 1.0% to about 4.0%, from about 1 .0% to about 5.0%, from about 1.0% to example but 6.0%, from about 1.0% to about 7.0%, from about 1.0% to about 7.5%, from about 1.0% to about 8.0%, from about 1.0 % to about 9.0%, from about 1.0% to about 10.0%, from about 2.0% to about 2.5%, from about 2.0% to about 3.0%, from about 2 0% to about 4.0%, from about 2.0% to about 5.0%, from about 2.0% to about 6.0%, from about 2.0% to about 7.0%, from about 2.0% to about 7.5%, from about 2.0% to about 8.0%, from about 2.0% to about 9.0%, from about 2.0% to about 10.0% , from about 5.0% to about 6.0%, from about 5.0% to about 7.0%, from about 5.0% to about 7.5%, from about 5.0% to about 8, 0%, about 5.0% to about 9.0%, about 5.0% to about 10.0%, about 5.0% to about 11.0%, about 5.0% to about 12.0%, from about 5.0% to about 13.0%, from about 5.0% to about 14.0%, from about 5.0% to about 15.0%, from about 6.0% to about 7.0%, from about 6.0% to about 7.5%, from about 6.0% to about 8.0%, from about 6.0% to about 9.0%, from about 6.0% to about 10.0%, from about 6.0% to about 11.0% , from about 6.0% to about 12.0%, from about 6.0% to about 13.0%, from about 6.0% to about 14.0%, or from about 6.0% to about 15, 0% by weight of the composition.

In another embodiment, the implementation of the composition described in this application does not contain a surfactant.

The preservative maintains the stability of the composition described in this application. Preservatives can also prevent the growth of microorganisms in the composition described in this application. Non-limiting examples of preservatives include methyl paraben, phenoxyethanol, capryl glycol, glyceryl caprylate, benzoic acid, sorbic acid, gallic acid, and propyl paraben.

The pH of the composition described in this application can be adjusted to any pH that allows the composition described in this application to achieve the desired beneficial effect. In aspects according to said embodiment, the pH of the composition described herein is, for example, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least at least 8, at least 9, at least 10, or at least 11. In other aspects, according to the specified embodiment, the pH of the composition described in this application is, for example, no more than 2, no more than 3, no more than 4, no more more than 5, no more than 6, no more than 7, no more than 8, no more than 9, no more than 10 or no more than 11.

In other aspects, according to the specified embodiment, the pH of the composition described in this application is, for example, from about 2 to about 5, from about 2 to about 5.5, from about 2 to about 6, from about 2 to about 6.5 , from about 2 to about 7, from about 2 to about 7.5, from about 2 to about 8, from about 2 to about 8.5, from about 2 to about 9, from about 2.5 to about 5, from about 2.5 to about 5.5, about 2.5 to about 6, about 2.5 to about 6.5, about 2.5 to about 7, about 2.5 to about 7.5, about 2.5 to about 8, about 2.5 to about 8.5, about 2.5 to about 9, about 3 to about 5, about 3 to about 5.5, about 3 to about 6, from about 3 to about 6.5, from about 3 to about 7, from about 3 to about 7.5, from about 3 to about 8, from about 3 to about 8.5, from about 3 to about 9, about 3.5 to about 5, about 3.5 to about 5.5, from about 3.5 to about 6, from about 3.5 to about 6.5, from about 3.5 to about 7, from about 3.5 to about 7.5, from about 3.5 to about 8, from about 3.5 to about 8.5, from about 3.5 to about 9, from about 4 to about 5, from about 4 to about 5.5, from about 4 to about 6, from about 4 to about 6.5, from about 4 to about 7, from about 4 to about 7.5, from about 4 to about 8, from about 4 to about 8.5, from about 4 to about 9, from about 4.5 to about 5, from about 4.5 to about 5.5, from about 4.5 to about 6, from about 4.5 to about 6.5, from about 4.5 to about 7, from about 4.5 to about 7.5, about 4.5 to about 8, about 4.5 to about 8.5, about 4.5 to about 9, about 5 to about 5.5, about 5 to about 6, about 5 to about 6.5, about 5 to about 7, about 5 to about 7.5, about 5 to about 8, about 5 to about 8 .5, about 5 to about 9, about 5.5 to about 6, about 5.5 to about 6.5, about 5.5 to about 7, about 5.5 to about 7.5, about 5.5 to about 8, about 5.5 to about 8.5, about 5.5 to about 9, about 6 to about 6.5, about 6 to about 7, about 6 to about 7.5, from about 6 to about 8, from at

- 28 042144 6 to about 8.5, about 6 to about 9, about 6.5 to about 7, about 6.5 to about 7.5, about 6.5 to about 8, about 6 .5 to about 8.5, from about 6.5 to about 9, from about 7 to about 7.5, from about 7 to about 8, from about 7 to about 8.5, from about 7 to about 9, from about 7.5 to about 8, about 7.5 to about 8.5, about 7.5 to about 9, about 8 to about 8.5, about 8 to about 9, or about 8.5 to about 9.

In other aspects, according to the specified embodiment, the pH of the composition described in this application is, for example, from about 6 to about 8, from about 6.7 to about 7.7, from about 6.8 to about 7.6, from about 6.9 to about 7.5, about 7.0 to about 7.4, about 7.1 to about 7.3, or about 7.2. In one aspect, according to said embodiment, the pH of the composition described herein is, for example, from about 6 to about 8, from about 6.7 to about 7.7, from about 6.8 to about 7.6, from about 6.9 to about 7.5, about 7.0 to about 7.4, about 7.1 to about 7.3, or about 7.2. In another embodiment, the implementation of the pH of the composition described in this application is, for example, from about 5.0 to about 6.5, from about 5.2 to about 6.2, from about 5.3 to about 6.1, from about 5.4 to about 6.0, about 5.5 to about 5.9, about 5.6 to about 5.8, or about 5.7. In one aspect, according to said embodiment, the pH of the composition described herein is, for example, from about 5.0 to about 6.5, from about 5.2 to about 6.2, from about 5.3 to about 6 .1, from about 5.4 to about 6.0, from about 5.5 to about 5.9, from about 5.6 to about 5.8, or about 5.7.

The composition described in this application is stable. In one embodiment, a composition described herein is stable when it exhibits, for example, no more than 1% degradation, no more than 2% degradation, no more than 3% degradation, no more than 4% degradation, no more than 5% degradation , no more than 6% decomposition, no more than 7% decomposition, no more than 8% decomposition, no more than 9% decomposition, or no more than 10% decomposition of hypochlorous acid. In another embodiment, the composition described in this application is stable when it shows, for example, no more than 10% decomposition, no more than 15% decomposition, no more than 20% decomposition, no more than 25% decomposition, no more than 30% decomposition, no more than 35% decomposition, no more than 40% decomposition, no more than 45% decomposition, no more than 50% decomposition, no more than 55% decomposition, no more than 60% decomposition, no more than 65% decomposition, no more than 70% decomposition, no more 75% decomposition, no more than 80% decomposition, no more than 85% decomposition, no more than 90% decomposition, or no more than 95% decomposition of hypochlorous acid. In another embodiment, a composition described herein is stable when it exhibits, for example, about 1% to about 10% degradation, about 1% to about 20% degradation, about 1% to about 30% degradation, from about 1% to about 40% degradation, from about 1% to about 50% degradation, from about 1% to about 60% degradation, from about 1% to about 70% degradation, from about 1% to about 80% degradation, from about 1% to about 90% degradation, from about 1% to about 95% degradation, from about 5% to about 10% degradation, from about 5% to about 20% degradation, from about 5% to about 30% degradation, about 5% to about 40% degradation, from about 5% to about 50% degradation, from about 5% to about 60% degradation, from about 5% to about 70% degradation, from about 5% to about 80% degradation, about 5% to about 90% degradation, from about 5% to about 95% degradation, from about 1 0% to about 20% degradation, about 10% to about 30% degradation, about 10% to about 40% degradation, about 10% to about 50% degradation, about 10% to about 60% degradation, from about 10% to about 70% degradation, about 10% to about 80% degradation, about 10% to about 90% degradation, about 10% to about 95% degradation, 20% to about 30% degradation, from about 20% to about 40% degradation, from about 20% to about 50% degradation, from about 20% to about 60% degradation, from about 20% to about 70% degradation, from about 20% to about 80% degradation, from about 20% to about 90% degradation, from about 20% to about 95% degradation, from about 30% to about 40% degradation, from about 30% to about 50% degradation, from about 30% to about 60% degradation, from about 30% to about 70% degradation, from about 30% to about 80% degradation, from about 30% to about 90% degradation, from about 3 0% to about 95% degradation, about 40% to about 50% degradation, about 40% to about 60% degradation, about 40% to about 70% degradation, about 40% to about 80% degradation, from about 40% to about 90% degradation, about 40% to about 95% degradation, about 50% to about 60% degradation, about 50% to about 70% degradation, about 50% to about 80% degradation, from about 50% to about 90% degradation, about 50% to about 95% degradation, about 60% to about 70% degradation, about 60% to about 80% degradation, about 60% to about 90% degradation, from about 60% to about 95% degradation, about 70% to about 80% degradation, from about 70% to about 90% degradation, from about 70% to about 95% degradation, from about 80% to about 90% degradation, from about 80% to about

- 29 042144

95% decomposition or from about 90% to about 95% decomposition of hypochlorous acid.

In one embodiment, a composition described herein is stable when it exhibits, for example, no more than 1% degradation, no more than 2% degradation, no more than 3% degradation, no more than 4% degradation, no more than 5% degradation , no more than 6% decomposition, no more than 7% decomposition, no more than 8% decomposition, no more than 9% decomposition, or no more than 10% decomposition of free active chlorine. In another embodiment, the composition described in this application is stable when it shows, for example, no more than 10% decomposition, no more than 15% decomposition, no more than 20% decomposition, no more than 25% decomposition, no more than 30% decomposition, no more than 35% decomposition, no more than 40% decomposition, no more than 45% decomposition, no more than 50% decomposition, no more than 55% decomposition, no more than 60% decomposition, no more than 65% decomposition, no more than 70% decomposition, no more 75% decomposition, no more than 80% decomposition, no more than 85% decomposition, no more than 90% decomposition, or no more than 95% decomposition of free active chlorine. In another embodiment, a composition described herein is stable when it exhibits, for example, about 1% to about 10% degradation, about 1% to about 20% degradation, about 1% to about 30% degradation, from about 1% to about 40% degradation, from about 1% to about 50% degradation, from about 1% to about 60% degradation, from about 1% to about 70% degradation, from about 1% to about 80% degradation, from about 1% to about 90% degradation, from about 1% to about 95% degradation, from about 5% to about 10% degradation, from about 5% to about 20% degradation, from about 5% to about 30% degradation, about 5% to about 40% degradation, from about 5% to about 50% degradation, from about 5% to about 60% degradation, from about 5% to about 70% degradation, from about 5% to about 80% degradation, about 5% to about 90% degradation, from about 5% to about 95% degradation, from about 1 0% to about 20% degradation, about 10% to about 30% degradation, about 10% to about 40% degradation, about 10% to about 50% degradation, about 10% to about 60% degradation, from about 10% to about 70% degradation, about 10% to about 80% degradation, about 10% to about 90% degradation, about 10% to about 95% degradation, 20% to about 30% degradation, from about 20% to about 40% degradation, from about 20% to about 50% degradation, from about 20% to about 60% degradation, from about 20% to about 70% degradation, from about 20% to about 80% degradation, from about 20% to about 90% degradation, from about 20% to about 95% degradation, from about 30% to about 40% degradation, from about 30% to about 50% degradation, from about 30% to about 60% degradation, from about 30% to about 70% degradation, from about 30% to about 80% degradation, from about 30% to about 90% degradation, from about 3 0% to about 95% degradation, about 40% to about 50% degradation, about 40% to about 60% degradation, about 40% to about 70% degradation, about 40% to about 80% degradation, from about 40% to about 90% degradation, about 40% to about 95% degradation, about 50% to about 60% degradation, about 50% to about 70% degradation, about 50% to about 80% degradation, from about 50% to about 90% degradation, about 50% to about 95% degradation, about 60% to about 70% degradation, about 60% to about 80% degradation, about 60% to about 90% degradation, from about 60% to about 95% degradation, about 70% to about 80% degradation, from about 70% to about 90% degradation, from about 70% to about 95% degradation, from about 80% to about 90% degradation, from about 80% to about 95% decomposition, or from about 90% to about 95% decomposition of free active chlorine.

In aspects according to the specified embodiment, the composition described in this application is stable for, for example, about 1 day, about 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days or 15 days. In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, at least 1 day, at least 2 days, at least 3 days, at least 4 days, at least 5 days , at least 6 days, at least 7 days, at least 8 days, at least 9 days, at least 10 days, at least 11 days, at least 12 days, at least 13 days, at least 14 days or at least 15 days. In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, no more than 1 day, no more than 2 days, no more than 3 days, no more than 4 days, no more than 5 days, no more than 6 days , no more than 7 days, no more than 8 days, no more than 9 days, no more than 10 days, no more than 11 days, no more than 12 days, no more than 13 days, no more than 14 days, or no more than 15 days.

In other aspects of this embodiment, the composition described in this application is stable for, for example, from about 1 day to about 2 days, from about 1 day to about 3 days, from about 1 day to about 4 days, from about 1 days to about 5 days, from about 1 day to about 6 days, from about 1 day to about 7 days, from about 1 day to about 8 days, from about 1 day to about 9 days, from about 1 day to about 10 days , from about 1 day to about 11 days, from about 1 day to about 12 days, from about 1 day to about 13

- 30 042144 days, from about 1 day to about 14 days, from about 1 day to about 15 days, from about 2 days to about 3 days, from about 2 days to about 4 days, from about 2 days to about 5 days, from about 2 days to about 6 days, from about 2 days to about 7 days, from about 2 days to about 8 days, from about 2 days to about 9 days, from about 2 days to about 10 days, from about 2 days to about 11 days, from about 2 days to about 12 days, from about 2 days to about 13 days, from about 2 days to about 14 days, from about 2 days to about 15 days, from about 3 days to about 4 days, from about 3 days to about 5 days, about 3 days to about 6 days, from about 3 days to about 7 days, from about 3 days to about 8 days, from about 3 days to about 9 days, from about 3 days to about 10 days, from about 3 days to about 11 days, from about 3 days to about 12 days, from about 3 days to about 13 days th, from about 3 days to about 14 days, from about 3 days to about 15 days, from about 4 days to about 5 days, from about 4 days to about 6 days, from about 4 days to about 7 days, from about 4 days to about 8 days, from about 4 days to about 9 days, from about 4 days to about 10 days, from about 4 days to about 11 days, from about 4 days to about 12 days, from about 4 days to about 13 days , from about 4 days to about 14 days, from about 4 days to about 15 days, from about 5 days to about 6 days, from about 5 days to about 7 days, from about 5 days to about 8 days, from about 5 days up to about 9 days, from about 5 days to about 10 days, from about 5 days to about 11 days, from about 5 days to about 12 days, from about 5 days to about 13 days, from about 5 days to about 14 days, from about 5 days to about 15 days, from about 6 days to about 7 days, from about 6 days to about 8 days, from about 6 days to about 9 days, about 6 days to about 10 days, about 6 days to about 11 days, about 6 days to about 12 days, about 6 days to about 13 days, about 6 days to about 14 days, from about 6 days to about 15 days, from about 7 days to about 8 days, from about 7 days to about 9 days, from about 7 days to about 10 days, from about 7 days to about 11 days, from about 7 days to about 12 days, about 7 days to about 13 days, from about 7 days to about 14 days, from about 7 days to about 15 days, from about 8 days to about 9 days, from about 8 days to about 10 days, from about 8 days to about 11 days, from about 8 days to about 12 days, from about 8 days to about 13 days, from about 8 days to about 14 days, from about 8 days to about 15 days, from about 9 days to about 10 days, from about 9 days to about 11 days, from about 9 days to about 12 days, from about 9 days to about 13 days, from about 9 days to about 14 days, from about 9 days to about 15 days, from about 10 days to about 11 days, from about 10 days to about 12 days, from about 10 days to about 13 days, from about 10 days to about 14 days, from about 10 days to about 15 days, from about 11 days to about 12 days, from about 11 days to about 13 days, from about 11 days to about 14 days, from about 11 days to about 15 days, from about 12 days to about 13 days, from about 12 days to about 14 days, from about 12 days to about 15 days, from about 13 days to about 14 days, from about 13 days to about 15 days or from about 14 days to about 15 days.

In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, about 1 week, about 2 weeks, 3 weeks, 4 weeks, 5 weeks, 6 weeks, 7 weeks, 8 weeks, 9 weeks, 10 weeks, 11 weeks, 12 weeks, 13 weeks, 14 weeks or 15 weeks. In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, at least 1 week, at least 2 weeks, at least 3 weeks, at least 4 weeks, at least 5 weeks , at least 6 weeks, at least 7 weeks, at least 8 weeks, at least 9 weeks, at least 10 weeks, at least 11 weeks, at least 12 weeks, at least 13 weeks, at least 14 weeks or at least 15 weeks. In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, no more than 1 week, no more than 2 weeks, no more than 3 weeks, no more than 4 weeks, no more than 5 weeks, no more than 6 weeks , 7 weeks or less, 8 weeks or less, 9 weeks or less, 10 weeks or less, 11 weeks or less, 12 weeks or less, 13 weeks or less, 14 weeks or less, or 15 weeks or less.

In aspects according to the specified implementation variant, the composition described in this application is stable for, for example, from about 1 week to about 2 weeks, from about 1 week to about 3 weeks, from about 1 week to about 4 weeks, from about 1 weeks to about 5 weeks, from about 1 week to about 6 weeks, from about 1 week to about 7 weeks, from about 1 week to about 8 weeks, from about 1 week to about 9 weeks, from about 1 week to about 10 weeks , from about 1 week to about 11 weeks, from about 1 week to about 12 weeks, from about 1 week to about 13 weeks, from about 1 week to about 14 weeks, from about 1 week to about 15 weeks, from about 2 weeks up to about 3 weeks, from about 2 weeks to about 4 weeks, from about 2 weeks to about 5 weeks, from about 2 weeks to about 6 weeks, from about 2 weeks to about 7 weeks, from about 2 weeks to about 8 weeks,from about 2 weeks to about 9 weeks, from about 2 weeks to

- 31 042144 at least 10 weeks, from about 2 weeks to about 11 weeks, from about 2 weeks to about 12 weeks, from about 2 weeks to about 13 weeks, from about 2 weeks to about 14 weeks, from about 2 weeks to about 15 weeks, from about 3 weeks to about 4 weeks, from about 3 weeks to about 5 weeks, from about 3 weeks to about 6 weeks, from about 3 weeks to about 7 weeks, from about 3 weeks to about 8 weeks, from about 3 weeks to about 9 weeks, from about 3 weeks to about 10 weeks, from about 3 weeks to about 11 weeks, from about 3 weeks to about 12 weeks, from about 3 weeks to about 13 weeks, from about 3 weeks to about 14 weeks , from about 3 weeks to about 15 weeks, from about 4 weeks to about 5 weeks, from about 4 weeks to about 6 weeks, from about 4 weeks to about 7 weeks, from about 4 weeks to about 8 weeks, from about 4 weeks up to about 9 weeks, from about 4 weeks to about 10 weeks, from about 4 weeks to about 11 weeks, from about 4 weeks to about 12 weeks, from about 4 weeks to about 13 weeks, from about 4 weeks to about 14 weeks, from about 4 weeks to about 15 weeks , from about 5 weeks to about 6 weeks, from about 5 weeks to about 7 weeks, from about 5 weeks to about 8 weeks, from about 5 weeks to about 9 weeks, from about 5 weeks to about 10 weeks, from about 5 weeks up to about 11 weeks, from about 5 weeks to about 12 weeks, from about 5 weeks to about 13 weeks, from about 5 weeks to about 14 weeks, from about 5 weeks to about 15 weeks, from about 6 weeks to about 7 weeks, from about 6 weeks to about 8 weeks, from about 6 weeks to about 9 weeks, from about 6 weeks to about 10 weeks, from about 6 weeks to about 11 weeks, from about 6 weeks to about 12 weeks, from about 6 weeks to approximately 13 weeks, from example but 6 weeks to about 14 weeks, about 6 weeks to about 15 weeks, from about 7 weeks to about 8 weeks, from about 7 weeks to about 9 weeks, from about 7 weeks to about 10 weeks, from about 7 weeks to about 11 weeks, from about 7 weeks to about 12 weeks, from about 7 weeks to about 13 weeks, from about 7 weeks to about 14 weeks, from about 7 weeks to about 15 weeks, from about 8 weeks to about 9 weeks, from about 8 weeks to about 10 weeks, about 8 weeks to about 11 weeks, from about 8 weeks to about 12 weeks, from about 8 weeks to about 13 weeks, from about 8 weeks to about 14 weeks, from about 8 weeks to about 15 weeks, from about 9 weeks to about 10 weeks, from about 9 weeks to about 11 weeks, from about 9 weeks to about 12 weeks, from about 9 weeks to about 13 weeks, from about 9 weeks to about 14 weeks, from about 9 weeks to about 15 weeks, from about 10 weeks to about 11 weeks, from about 10 weeks to about 12 weeks, from about 10 weeks to about 13 weeks, from about 10 weeks to about 14 weeks, from about 10 weeks to about 15 weeks, from about 11 weeks to about 12 weeks, from about 11 weeks to about 13 weeks, from about 11 weeks to about 14 weeks, from about 11 weeks to about 15 weeks, from about 12 weeks to about 13 weeks, from about 12 weeks to about 14 weeks, from about 12 weeks to about 15 weeks, from about 13 weeks to about 14 weeks, from about 13 weeks to about 15 weeks, or from about 14 weeks to about 15 weeks.

In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, about 1 month, about 2 months, 3 months, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 12 months, 13 months, 14 months, 15 months, 16 months, 17 months or 18 months. In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, at least 1 month, at least 2 months, at least 3 months, at least 4 months, at least 5 months at least 6 months at least 7 months at least 8 months at least 9 months at least 10 months at least 11 months at least 12 months at least 13 months at least 14 months, at least 15 months, at least 16 months, at least 17 months, or at least 18 months. In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, no more than 1 month, no more than 2 months, no more than 3 months, no more than 4 months, no more than 5 months, no more than 6 months , no more than 7 months, no more than 8 months, no more than 9 months, no more than 10 months, no more than 11 months, no more than 12 months, no more than 13 months, no more than 14 months, no more than 15 months, no more than 16 months , not more than 17 months or not more than 18 months.

In aspects according to the specified implementation variant, the composition described in this application is stable for, for example, from about 1 month to about 2 months, from about 1 month to about 3 months, from about 1 month to about 4 months, from about 1 about 1 month to about 6 months, about 1 month to about 7 months, about 1 month to about 8 months, about 1 month to about 9 months, about 1 month to about 10 months , from about 1 month to about 11 months, from about 1 month to about 12 months, from about 1 month to about 13 months, from about 1 month to about 14 months, from about 1 month to about 15 months, from about 1 month before at

- 32 042144 at least 16 months, from about 1 month to about 17 months, from about 1 month to about 18 months, from about 2 months to about 3 months, from about 2 months to about 4 months, from about 2 months to about 5 months, from about 2 months to about 6 months, from about 2 months to about 7 months, from about 2 months to about 8 months, from about 2 months to about 9 months, from about 2 months to about 10 months, from about 2 months to about 11 months, from about 2 months to about 12 months, from about 2 months to about 13 months, from about 2 months to about 14 months, from about 2 months to about 15 months, from about 2 months to about 16 months , from about 2 months to about 17 months, from about 2 months to about 18 months, from about 3 months to about 4 months, from about 3 months to about 5 months, from about 3 months to about 6 months, from about 3 me months to about 7 months, from about 3 months to about 8 months, from about 3 months to about 9 months, from about 3 months to about 10 months, from about 3 months to about 11 months, from about 3 months to about 12 months , from about 3 months to about 13 months, from about 3 months to about 14 months, from about 3 months to about 15 months, from about 3 months to about 16 months, from about 3 months to about 17 months, from about 3 months up to about 18 months, from about 4 months to about 5 months, from about 4 months to about 6 months, from about 4 months to about 7 months, from about 4 months to about 8 months, from about 4 months to about 9 months, from about 4 months to about 10 months, from about 4 months to about 11 months, from about 4 months to about 12 months, from about 4 months to about 13 months, from about 4 months to about 14 months, from about 4 months to about 15 months, about 4 months to about 16 months, from about 4 months to about 17 months, from about 4 months to about 18 months, from about 5 months to about 6 months, from about 5 months to about 7 months, from about 5 months to about 8 months, from about 5 months to about 9 months, from about 5 months to about 10 months, from about 5 months to about 11 months, from about 5 months to about 12 months, from about 5 months to about 13 months, from about 5 months to about 14 months, from about 5 months to about 15 months, from about 5 months to about 16 months, from about 5 months to about 17 months, from about 5 months to about 18 months , from about 6 months to about 7 months, from about 6 months to about 8 months, from about 6 months to about 9 months, from about 6 months to about 10 months, from about 6 months to about 11 months, from approx. 6 months to about 12 months, about 6 months to about 13 months, about 6 months to about 14 months, about 6 months to about 15 months, about 6 months to about 16 months, about 6 months to about 17 months, from about 6 months to about 18 months, from about 7 months to about 8 months, from about 7 months to about 9 months, from about 7 months to about 10 months, from about 7 months to about 11 months, from about 7 months to about 12 months, about 7 months to about 13 months, from about 7 months to about 14 months, from about 7 months to about 15 months, from about 7 months to about 16 months, from about 7 months to about 17 months, from about 7 months to about 18 months, from about 8 months to about 9 months, from about 8 months to about 10 months, from about 8 months to about 11 months, from about 8 months to about 12 months , from about 8 months to about 13 months, from about 8 months to about 14 months, from about 8 months to about 15 months, from about 8 months to about 16 months, from about 8 months to about 17 months, from about 8 months up to about 18 months, from about 9 months to about 10 months, from about 9 months to about 11 months, from about 9 months to about 12 months, from about 9 months to about 13 months, from about 9 months to about 14 months, from about 9 months to about 15 months, from about 9 months to about 16 months, from about 9 months to about 17 months, from about 9 months to about 18 months, from about 10 months to about 11 months, from about 10 months to about 12 months, from about 10 months to about 13 months, from about 10 months to about 14 months, from about 10 months to about 15 months, from about 10 months to about 16 months, from about 10 months to about 17 months, from about 10 months to about 18 months, from about 11 months to about 12 months, from about 11 months to about 13 months, from about 11 months to about 14 months, from about 11 months to about 15 months, from about 11 months to about 16 months, from about 11 months to about 17 months, from about 1 month to about 18 months, from about 12 months to about 13 months, from about 12 months to about 14 months, from about 12 months to about 15 months, from about 12 months to about 16 months, from about 12 months to about 17 months, from about 12 months to about 18 months, from about 13 months to about 14 months, from about 13 months to about 15 months, from about 13 months to about 16 months, from about 13 months to about 17 months, from about 13 months to about 18 months, from about 14 months to about 15 months, from about 14 months to about 16 months months, from about 14 months to about 17 months, from about 14 months to about 18 months

- 33 042144 months, from about 15 months to about 16 months, from about 15 months to about 17 months, from about 15 months to about 18 months, from about 16 months to about 17 months, from about 16 months to about 18 months, or from about 17 months to about 18 months.

In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, about 1 year, about 2 years, about 3 years, about 4 years, or about 5 years. In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, at least 1 year, at least 2 years, at least 3 years, at least 4 years, or at least 5 years . In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, no more than 1 year, no more than 2 years, no more than 3 years, no more than 4 years, or no more than 5 years. In other aspects, according to the specified embodiment, the composition described in this application is stable for, for example, from about 1 year to about 2 years, from about 1 year to about 3 years, from about 1 year to about 4 years, from about 1 year to about 5 years, about 2 years to about 3 years, from about 2 years to about 4 years, from about 2 years to about 5 years, from about 3 years to about 4 years, from about 3 years to about 5 years or from about 4 years to about 5 years.

The composition described in this application can be obtained in any form that allows you to apply the composition described in this application, so as to achieve the desired beneficial effect. In one embodiment, the composition described in this application can be obtained, for example, in the form of a single-phase composition or a two-phase composition containing a medium phase and a dispersed phase. In another embodiment, the implementation of the composition described in this application, you can get, for example, in the form of a liquid composition, colloidal composition, semi-solid composition or solid composition. In another embodiment, the implementation of the composition described in this application can be obtained, for example, in the form of an aerosol of liquid particles, foam, emulsion, gel, sol or solid sol. In another embodiment, the implementation of the composition described in this application, you can get, for example, in the form of a spray, aerosol liquid particles, soap or suspension.

In one embodiment, the composition described herein contains from about 80 ppm to about 115 ppm of hypochlorous acid or free active chlorine and from about 0.01% to about 0.5% dialgedide. In aspects according to the specified variant implementation of the composition described in this application contains from about 85 ppm to about 110 ppm of hypochlorous acid or free active chlorine and from about 0.025% to about 0.4% dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 90 ppm to about 105 ppm of hypochlorous acid or free active chlorine and from about 0.05% to about 0.3% dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 95 ppm to about 100 ppm of hypochlorous acid or free active chlorine and from about 0.075% to about 0.2% dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains about 97 ppm of hypochlorous acid or free active chlorine and about 0.1% dialdehyde.

In one embodiment, the composition described herein contains from about 80 ppm to about 115 ppm hypochlorous acid or free available chlorine and from about 0.01% to about 0.5% glutaraldehyde. In aspects according to the specified variant implementation of the composition described in this application contains from about 85 ppm to about 110 ppm of hypochlorous acid or free active chlorine and from about 0.025% to about 0.4% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 90 ppm to about 105 ppm of hypochlorous acid or free active chlorine and from about 0.05% to about 0.3% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 95 ppm to about 100 ppm of hypochlorous acid or free active chlorine and from about 0.075% to about 0.2% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains about 97 ppm of hypochlorous acid or free active chlorine and about 0.1% glutaraldehyde.

In one embodiment, the composition described herein contains from about 80 ppm to about 115 ppm hypochlorous acid or free active chlorine and from about 0.05% to about 0.6% dialdehyde. In aspects of this embodiment, the composition described herein contains from about 85 ppm to about 110 ppm hypochlorous acid or free active chlorine and from about 0.075% to about 0.5% dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 90 ppm to about 105 ppm of hypochlorous acid or free active chlorine and from about 0.1% to about 0.4% dialdehyde. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 95 ppm to about 100 ppm of hypochlorous acid or free active chlorine and from about 0.2% to about

- 34 042144

0.3% dialdehyde. In other aspects, according to the specified variant implementation of the composition described in this application contains about 97 ppm of hypochlorous acid or free active chlorine and about 0.25% dialdehyde.

In one embodiment, the composition described herein contains from about 80 ppm to about 115 ppm hypochlorous acid or free active chlorine and from about 0.05% to about 0.6% glutaraldehyde. In aspects according to the specified variant implementation of the composition described in this application contains from about 85 ppm to about 110 ppm of hypochlorous acid or free active chlorine and from about 0.075% to about 0.5% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 90 ppm to about 105 ppm of hypochlorous acid or free active chlorine and from about 0.1% to about 0.4% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 95 ppm to about 100 ppm of hypochlorous acid or free active chlorine and from about 0.2% to about 0.3% glutaraldehyde. In other aspects, according to the specified variant implementation of the composition described in this application contains about 97 ppm of hypochlorous acid or free active chlorine and about 0.25% glutaraldehyde.

In one embodiment, the composition described herein contains from about 80 ppm to about 115 ppm hypochlorous acid or free active chlorine and from about 0.1% to about 1.0% dialdehyde. In aspects of this embodiment, the composition described herein contains from about 85 ppm to about 110 ppm hypochlorous acid or free active chlorine and from about 0.2% to about 0.8% dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 90 ppm to about 105 ppm of hypochlorous acid or free active chlorine and from about 0.3% to about 0.7% dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 95 ppm to about 100 ppm of hypochlorous acid or free active chlorine and from about 0.4% to about 0.6% dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains about 97 ppm of hypochlorous acid or free active chlorine and about 0.5% dialdehyde.

In one embodiment, the composition described herein contains from about 80 ppm to about 115 ppm hypochlorous acid or free active chlorine and from about 0.1% to about 1.0% glutaraldehyde. In aspects of this embodiment, the composition described herein contains from about 85 ppm to about 110 ppm hypochlorous acid or free active chlorine and from about 0.2% to about 0.8% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 90 ppm to about 105 ppm of hypochlorous acid or free active chlorine and from about 0.3% to about 0.7% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 95 ppm to about 100 ppm of hypochlorous acid or free active chlorine and from about 0.4% to about 0.6% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains about 97 ppm of hypochlorous acid or free active chlorine and about 0.5% glutaraldehyde.

In one embodiment, the composition described herein contains from about 4% to about 12% of a compound containing a biguanide moiety, from about 60% to about 80% alcohol, and from about 0.1% to about 5.0% dialdehyde . In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% of a compound containing a biguanide fragment, from about 62% to about 78% alcohol, and from about 0.5% to about 3.5% dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 6% to about 10% of a compound containing a biguanide fragment, from about 65% to about 75% alcohol, and from about 1.0% to about 3.0 % dialdehyde. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% of a compound containing a biguanide fragment, from about 67% to about 73% alcohol, and from about 1.5% to about 2.5 % dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains about 8% of the compound containing the biguanide fragment, about 70% alcohol and about 2.0% dialdehyde.

In one embodiment, the composition described herein contains from about 4% to about 12% polyhexamethylene biguanide, from about 60% to about 80% isopropanol, and from about 0.1% to about 5.0% glutaraldehyde. In aspects of this embodiment, the composition described herein contains from about 5% to about 11% polyhexamethylene biguanide, from about 62% to about 78% isopropanol, and from about 0.5% to about 3.5% glutaraldehyde. In other aspects, according to the specified embodiment, the composition,

- 35 042144 described in this application contains from about 6% to about 10% polyhexamethylene biguanide, from about 65% to about 75% isopropanol and from about 1.0% to about 3.0% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide, from about 67% to about 73% isopropanol and from about 1.5% to about 2.5% glutaraldehyde. In other aspects, according to the specified embodiment, the composition described in this application contains about 8% polyhexamethylene biguanide, about 70% isopropanol and about 2.0% glutaraldehyde.

In one embodiment, the composition described herein contains from about 5% to about 15% of a compound containing a biguanide moiety, from about 60% to about 80% alcohol, and from about 0.1% to about 1.0% dialdehyde . In aspects according to the specified implementation variant, the composition described in this application contains from about 6.5% to about 12.5% of a compound containing a biguanide fragment, from about 62% to about 78% alcohol and from about 0.2% to about 0.8% dialdehyde. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7.5% to about 11.5% of a compound containing a biguanide fragment, from about 65% to about 75% alcohol and from about 0.3% to about 0.7% dialdehyde. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8.5% to about 10.5% of a compound containing a biguanide fragment, from about 67% to about 73% alcohol and from about 0.4% to about 0.6% dialdehyde. In other aspects, according to the specified embodiment, the composition described in this application contains about 9.5% of the compound containing the biguanide fragment, about 70% alcohol and about 0.5% dialdehyde.

In one embodiment, the composition described herein contains about 5% to about 15% polyhexamethylene biguanide, about 60% to about 80% isopropanol, and about 0.1% to about 1.0% o-phthalaldehyde. In aspects according to the specified embodiment, the composition described in this application contains from about 6.5% to about 12.5% polyhexamethylene biguanide, from about 62% to about 78% isopropanol, and from about 0.2% to about 0.8% ortho-phthalaldehyde. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7.5% to about 11.5% polyhexamethylene biguanide, from about 65% to about 75% isopropanol, and from about 0.3% to about 0.7 % ortho-phthalaldehyde. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8.5% to about 10.5% polyhexamethylene biguanide, from about 67% to about 73% isopropanol, and from about 0.4% to about 0.6 % orthophthalaldehyde. In other aspects, according to the specified implementation variant, the composition described in this application contains about 9.5% polyhexamethylene biguanide, about 70% isopropanol and about 0.5% ortho-phthalaldehyde.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety, from about 60% to about 80% alcohol, and from about 1% to about 10% surfactant per based on quaternary ammonium. In aspects of this embodiment, the composition described herein comprises from about 2% to about 8% of a compound containing a biguanide moiety, from about 62% to about 78% alcohol, and from about 2% to about 8% of a surfactant based on quaternary ammonium. In other aspects, according to the specified embodiment, the composition described in this application contains from about 3% to about 7% of a compound containing a biguanide fragment, from about 65% to about 75% alcohol and from about 3% to about 7% surfactant substances based on quaternary ammonium. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 6% of a compound containing a biguanide fragment, from about 67% to about 73% alcohol and from about 4% to about 6% surfactant substances based on quaternary ammonium. In other aspects, according to the specified implementation variant, the composition described in this application contains about 5% of the compound containing the biguanide fragment, about 70% alcohol and about 5% surfactant based Quaternary ammonium.

In one embodiment, the composition described herein contains about 1% to about 10% polyhexamethylene biguanide, about 60% to about 80% isopropanol, and about 1% to about 10% benzalkonium chloride. In aspects of this embodiment, the composition described herein comprises about 2% to about 8% polyhexamethylene biguanide, about 62% to about 78% isopropanol, and about 2% to about 8% benzalkonium chloride. In other aspects, according to the specified embodiment, the composition described in this application contains from about 3% to about 7% polyhexamethylene biguanide, from about 65% to about 75% isopropanol and from about 3% to about 7% benzalkonium chloride. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 6% polyhexamethylene biguanide, from about 67%

- 36 042144 to about 73% isopropanol and from about 4% to about 6% benzalkonium chloride. In other aspects, according to the specified embodiment, the composition described in this application contains about 5% polyhexamethylene biguanide, about 70% isopropanol and about 5% benzalkonium chloride.

In one embodiment, the composition described herein contains from about 5% to about 15% of a compound containing a biguanide moiety, from about 55% to about 75% alcohol, and from about 1% to about 10% surfactant per based on quaternary ammonium. In aspects of this embodiment, the composition described herein comprises from about 7% to about 13% of a compound containing a biguanide moiety, from about 57% to about 73% alcohol, and from about 2% to about 8% of a surfactant based on quaternary ammonium. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% of a compound containing a biguanide fragment, from about 60% to about 70% alcohol and from about 3% to about 7% surfactant substances based on quaternary ammonium. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of a compound containing a biguanide fragment, from about 62% to about 68% alcohol and from about 4% to about 6% surfactant substances based on quaternary ammonium. In other aspects, according to the specified embodiment, the composition described in this application contains about 10% of a compound containing a biguanide moiety, about 65% alcohol, and about 5% quaternary ammonium surfactant.

In one embodiment, the composition described herein contains about 5% to about 15% polyhexamethylene biguanide, about 55% to about 75% isopropanol, and about 1% to about 10% benzalkonium chloride. In aspects of this embodiment, the composition described herein comprises about 7% to about 13% polyhexamethylene biguanide, about 57% to about 73% isopropanol, and about 2% to about 8% benzalkonium chloride. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide, from about 60% to about 70% isopropanol and from about 3% to about 7% benzalkonium chloride. In other aspects, according to the specified embodiment, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide, from about 62% to about 68% isopropanol and from about 4% to about 6% benzalkonium chloride. In other aspects, according to the specified embodiment, the composition described in this application contains about 10% polyhexamethylene biguanide, about 65% isopropanol and about 5% benzalkonium chloride.

In one embodiment, the composition described herein contains from about 85 ppm to about 120 ppm of hypochlorous acid or free active chlorine and from about 1.0% to about 3.0% of a compound containing a biguanide moiety. In aspects of this embodiment, the composition described herein contains from about 90 ppm to about 115 ppm of hypochlorous acid or free active chlorine and from about 1.25% to about 2.75% of a compound containing a biguanide moiety. In other aspects, according to the specified embodiment, the composition described in this application contains from about 95 ppm to about 110 ppm of hypochlorous acid or free active chlorine and from about 1.5% to about 2.5% of the compound containing the biguanide moiety. In other aspects, according to the specified embodiment, the composition described in this application contains from about 100 ppm to about 106 ppm of hypochlorous acid or free active chlorine and from about 1.75% to about 2.25% of the compound containing the biguanide moiety. In other aspects, according to the specified implementation variant, the composition described in this application contains about 103 ppm of hypochlorous acid or free active chlorine and about 2.0% of the compound containing the biguanide fragment.

In one embodiment, the composition described in this application contains from about 85 ppm to about 120 ppm of hypochlorous acid or free active chlorine and from about 1.0% to about 3.0% of polyhexamethylene biguanide. In aspects according to the specified variant implementation of the composition described in this application contains from about 90 ppm to about 115 ppm of hypochlorous acid or free active chlorine and from about 1.25% to about 2.75% of polyhexamethylene biguanide. In other aspects, according to the specified embodiment, the composition described in this application contains from about 95 ppm to about 110 ppm of hypochlorous acid or free active chlorine and from about 1.5% to about 2.5% of polyhexamethylene biguanide. In other aspects, according to the specified embodiment, the composition described in this application contains from about 100 ppm to about 106 ppm of hypochlorous acid or free active chlorine and from about 1.75% to about 2.25% polyhexamethylene biguanide. In other aspects, according to the specified implementation variant, the composition described in this application contains

- 37 042144 about 103 ppm hypochlorous acid or free active chlorine and about 2.0% polyhexamethylene biguanide.

In one embodiment, the composition described herein contains from about 85 ppm to about 120 ppm of hypochlorous acid or free active chlorine and from about 0.5% to about 2.5% of a compound containing a biguanide moiety. In aspects of this embodiment, the composition described herein contains from about 90 ppm to about 115 ppm of hypochlorous acid or free active chlorine and from about 0.75% to about 2.25% of a compound containing a biguanide moiety. In other aspects, according to the specified embodiment, the composition described in this application contains from about 95 ppm to about 110 ppm of hypochlorous acid or free active chlorine and from about 1.0% to about 2.0% of the compound containing the biguanide moiety. In other aspects, according to the specified embodiment, the composition described in this application contains from about 100 ppm to about 106 ppm of hypochlorous acid or free active chlorine and from about 1.25% to about 1.75% of the compound containing the biguanide moiety. In other aspects, according to the specified embodiment, the composition described in this application contains about 103 ppm of hypochlorous acid or free active chlorine and about 1.5% of the compound containing the biguanide moiety.

In one embodiment, the composition described in this application contains from about 85 ppm to about 120 ppm of hypochlorous acid or free active chlorine and from about 0.5% to about 2.5% polyhexamethylene biguanide. In aspects according to the specified variant implementation of the composition described in this application contains from about 90 ppm to about 115 ppm of hypochlorous acid or free active chlorine and from about 0.75% to about 2.25% of polyhexamethylene biguanide. In other aspects, according to the specified embodiment, the composition described in this application contains from about 95 ppm to about 110 ppm of hypochlorous acid or free active chlorine and from about 1.0% to about 2.0% of polyhexamethylene biguanide. In other aspects, according to the specified embodiment, the composition described in this application contains from about 100 ppm to about 106 ppm of hypochlorous acid or free available chlorine and from about 1.25% to about 1.75% polyhexamethylene biguanide. In other aspects, according to the specified embodiment, the composition described in this application contains about 103 ppm of hypochlorous acid or free active chlorine and about 1.5% polyhexamethylene biguanide.

In one embodiment, the composition described herein contains from about 0.1% to about 7% of a compound containing a biguanide moiety and from about 60% to about 80% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 0.5% to about 6% of a compound containing a biguanide moiety and from about 62% to about 78% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 1% to about 5% of the compound containing the biguanide fragment, and from about 65% to about 75% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 2% to about 4% of the compound containing the biguanide fragment, and from about 67% to about 73% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 3% of the compound containing the biguanide fragment, and about 70% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described herein contains from about 0.1% to about 7% polyhexamethylene biguanide and from about 60% to about 80% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 0.5% to about 6% polyhexamethylene biguanide and from about 62% to about 78% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 1% to about 5% polyhexamethylene biguanide and from about 65% to about 75% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 2% to about 4% polyhexamethylene biguanide and from about 67% to about 73% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 3% polyhexamethylene biguanide and about 70% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, according to the specified version of the real

- 38 042144 compositions are used to clean, disinfect and/or sterilize an endoscope, an ECMO heart-lung machine or a hard surface, as described in this application.

In one embodiment, the composition described herein contains from about 0.1% to about 7% of a compound containing a biguanide moiety and from about 50% to about 70% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 0.5% to about 6% of a compound containing a biguanide moiety and from about 52% to about 68% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 1% to about 5% of the compound containing the biguanide fragment, and from about 55% to about 65% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 2% to about 4% of the compound containing the biguanide fragment, and from about 57% to about 63% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 3% of the compound containing the biguanide fragment, and about 60% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 0.1% to about 7% polyhexamethylene biguanide and from about 50% to about 70% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 0.5% to about 6% polyhexamethylene biguanide and from about 52% to about 68% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 1% to about 5% polyhexamethylene biguanide and from about 55% to about 65% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 2% to about 4% polyhexamethylene biguanide and from about 57% to about 63% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 3% polyhexamethylene biguanide and about 60% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, according to said embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 0.1% to about 7% of a compound containing a biguanide moiety and from about 40% to about 60% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 0.5% to about 6% of a compound containing a biguanide moiety and from about 42% to about 58% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 1% to about 5% of the compound containing the biguanide fragment, and from about 45% to about 55% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 2% to about 4% of the compound containing the biguanide fragment, and from about 47% to about 53% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 3% of the compound containing the biguanide fragment, and about 50% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 0.1% to about 7% polyhexamethylene biguanide and from about 40% to about 60% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 0.5% to about 6% polyhexamethylene biguanide and from about 42% to about 58% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 1% to about 5% polyhexamethylene biguanide and from about 45% to about 55% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 2% to about 4% polyhexamethylene biguanide and from about 47% to about 53% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains approximately 3%

- 39 042144 polyhexamethylene biguanide and about 50% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, according to said embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 15% of a compound containing a biguanide moiety and from about 20% to about 85% of one or more alcohols. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 13% of a compound containing a biguanide fragment, and from about 25% to about 80% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 12% of the compound containing the biguanide fragment, and from about 30% to about 75% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 11% of the compound containing the biguanide fragment, and from about 35% to about 70% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% of the compound containing the biguanide fragment, and from about 40% to about 65% of one or more alcohols. In other aspects, according to said embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 1% to about 15% polyhexamethylene biguanide and from about 20% to about 85% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 13% polyhexamethylene biguanide and from about 25% to about 80% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 4% to about 12% polyhexamethylene biguanide and from about 30% to about 75% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide and from about 35% to about 70% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide and from about 40% to about 60% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety and from about 60% to about 80% of one or more alcohols. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% of a compound containing a biguanide fragment, and from about 62% to about 78% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% of the compound containing the biguanide fragment, and from about 65% to about 75% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% of the compound containing the biguanide fragment, and from about 67% to about 73% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 6% of the compound containing the biguanide fragment, and about 70% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 1% to about 10% polyhexamethylene biguanide and from about 60% to about 80% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% polyhexamethylene biguanide and from about 62% to about 78% isopropanol. In other aspects, according to the specified implementation variant, the composition described

- 40 042144 in this application contains from about 4% to about 8% polyhexamethylene biguanide and from about 65% to about 75% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 5% to about 7% polyhexamethylene biguanide and from about 67% to about 73% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 6% polyhexamethylene biguanide and about 70% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety and from about 55% to about 75% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 3% to about 9% of a compound containing a biguanide moiety and from about 57% to about 73% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% of the compound containing the biguanide fragment, and from about 60% to about 70% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% of the compound containing the biguanide fragment, and from about 62% to about 68% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 6% of the compound containing the biguanide fragment, and about 65% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the implementation of the composition described in this application contains from about 1% to about 10% polyhexamethylene biguanide and from about 55% to about 75% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% polyhexamethylene biguanide and from about 57% to about 73% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 4% to about 8% polyhexamethylene biguanide and from about 60% to about 70% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 5% to about 7% polyhexamethylene biguanide and from about 62% to about 68% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 6% polyhexamethylene biguanide and about 65% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, according to said embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety and from about 50% to about 70% of one or more alcohols. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% of the compound containing the biguanide fragment, and from about 52% to about 68% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% of the compound containing the biguanide fragment, and from about 55% to about 65% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% of the compound containing the biguanide fragment, and from about 57% to about 63% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 6% of the compound containing the biguanide fragment, and about 60% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described herein contains from about 1% to about 10% polyhexamethylene biguanide and from about 50% to about 70% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% polyhexamethylene biguanide and from about 52% to about 68% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 4% to about 8% polyhexamethylene biguanide and from about 55% to about 65% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 5% to about 7% polyhexamethylene biguanide and from about 57% to about 63% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 6% polyhexamethylene biguanide and about 60% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety and from about 45% to about 65% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 3% to about 9% of a compound containing a biguanide moiety and from about 47% to about 63% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% of the compound containing the biguanide fragment, and from about 50% to about 60% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% of the compound containing the biguanide fragment, and from about 52% to about 58% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 6% of the compound containing the biguanide fragment, and about 55% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 1% to about 10% polyhexamethylene biguanide and from about 45% to about 65% isopropanol. In aspects of this embodiment, the composition described herein contains from about 3% to about 9% polyhexamethylene biguanide and from about 47% to about 63% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% polyhexamethylene biguanide and from about 50% to about 60% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 5% to about 7% polyhexamethylene biguanide and from about 52% to about 58% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 6% polyhexamethylene biguanide and about 55% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety and from about 40% to about 60% of one or more alcohols. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% of the compound containing the biguanide fragment, and from about 42% to about 58% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% of the compound containing the biguanide fragment, and from about 45% to about 55% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% of the compound containing the biguanide fragment, and from about 47% to about 53% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 6% of the compound containing the biguanide fragment, and about 50% of one or more alcohols. In other aspects, according to said embodiment, the compositions further comprise from about 2% to about 7% of a quaternary surfactant.

- 42 042144 ammonium. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 1% to about 10% polyhexamethylene biguanide and from about 40% to about 60% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% polyhexamethylene biguanide and from about 42% to about 58% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 4% to about 8% polyhexamethylene biguanide and from about 45% to about 55% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 5% to about 7% polyhexamethylene biguanide and from about 47% to about 53% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 6% polyhexamethylene biguanide and about 50% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety and from about 35% to about 55% of one or more alcohols. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% of the compound containing the biguanide fragment, and from about 37% to about 53% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% of the compound containing the biguanide fragment, and from about 40% to about 50% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% of the compound containing the biguanide fragment, and from about 42% to about 48% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 6% of a compound containing a biguanide fragment, and about 45% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 1% to about 10% polyhexamethylene biguanide and from about 35% to about 55% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% polyhexamethylene biguanide and from about 37% to about 53% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% polyhexamethylene biguanide and from about 40% to about 50% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 5% to about 7% polyhexamethylene biguanide and from about 42% to about 48% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 6% polyhexamethylene biguanide and about 45% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety and from about 30% to about 50% of one or more alcohols. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% of a compound containing a biguanide fragment, and from about 32% to about 48% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% of the compound containing the biguanide fragment, and from about 35% to about 45% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% of the compound containing the biguanide fragment, and from about 37% to about 43% of one or more alcohols. IN

- 43 042144 other aspects according to the specified embodiment, the composition described in this application contains about 6% of a compound containing a biguanide fragment, and about 40% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 1% to about 10% polyhexamethylene biguanide and from about 30% to about 50% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% polyhexamethylene biguanide and from about 32% to about 48% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 4% to about 8% polyhexamethylene biguanide and from about 35% to about 45% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% polyhexamethylene biguanide and from about 37% to about 43% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 6% polyhexamethylene biguanide and about 40% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety and from about 25% to about 45% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 3% to about 9% of a compound containing a biguanide moiety and from about 27% to about 43% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% of the compound containing the biguanide fragment, and from about 30% to about 40% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% of the compound containing the biguanide fragment, and from about 32% to about 38% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 6% of the compound containing the biguanide fragment, and about 35% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 1% to about 10% polyhexamethylene biguanide and from about 25% to about 45% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% polyhexamethylene biguanide and from about 27% to about 43% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 4% to about 8% polyhexamethylene biguanide and from about 30% to about 40% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 5% to about 7% polyhexamethylene biguanide and from about 32% to about 38% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 6% polyhexamethylene biguanide and about 35% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 3% to about 13% of a compound containing a biguanide moiety and from about 60% to about 80% of one or more alcohols. In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% of a compound containing a biguanide fragment, and from about 62% to about 78% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from

- 44 042144 about 6% to about 10% of a compound containing a biguanide fragment, and from about 65% to about 75% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% of the compound containing the biguanide fragment, and from about 67% to about 73% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% of the compound containing the biguanide fragment, and about 70% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 3% to about 13% polyhexamethylene biguanide and from about 60% to about 80% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide and from about 62% to about 78% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide and from about 65% to about 75% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide and from about 67% to about 73% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 8% polyhexamethylene biguanide and about 70% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 3% to about 13% of a compound containing a biguanide moiety and from about 55% to about 75% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 5% to about 11% of a compound containing a biguanide moiety and from about 57% to about 73% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% of a compound containing a biguanide fragment, and from about 60% to about 70% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% of the compound containing the biguanide fragment, and from about 62% to about 68% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% of the compound containing the biguanide fragment, and about 65% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 3% to about 13% polyhexamethylene biguanide and from about 55% to about 75% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide and from about 57% to about 73% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide and from about 60% to about 70% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide and from about 62% to about 68% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 8% polyhexamethylene biguanide and about 65% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 3% to about 13% of a compound containing a biguanide moiety, and from about 50% to

- 45 042144 measured 70% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 5% to about 11% of a compound containing a biguanide moiety and from about 52% to about 68% of one or more alcohols. In other aspects, according to the specified embodiment, the composition described in this application contains from about 6% to about 10% of a compound containing a biguanide fragment, and from about 55% to about 65% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% of the compound containing the biguanide fragment, and from about 57% to about 63% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% of the compound containing the biguanide fragment, and about 60% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described herein contains from about 3% to about 13% polyhexamethylene biguanide and from about 50% to about 70% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide and from about 52% to about 68% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide and from about 55% to about 65% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide and from about 57% to about 63% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 8% polyhexamethylene biguanide and about 60% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 3% to about 13% of a compound containing a biguanide moiety and from about 45% to about 65% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 5% to about 11% of a compound containing a biguanide moiety and from about 47% to about 63% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% of the compound containing the biguanide fragment, and from about 50% to about 60% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% of the compound containing the biguanide fragment, and from about 52% to about 58% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% of the compound containing the biguanide fragment, and about 55% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 3% to about 13% polyhexamethylene biguanide and from about 45% to about 65% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide and from about 47% to about 63% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide and from about 50% to about 60% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide and from about 52% to about 58% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 8% polyhexamethylene biguanide and about 55% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, according to the specified version of the real

- 46 042144 compositions are used to clean, disinfect and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described in this application.

In one embodiment, the composition described herein contains from about 3% to about 13% of a compound containing a biguanide moiety and from about 40% to about 60% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 5% to about 11% of a compound containing a biguanide moiety and from about 42% to about 58% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% of the compound containing the biguanide fragment, and from about 45% to about 55% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% of the compound containing the biguanide fragment, and from about 47% to about 53% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% of a compound containing a biguanide fragment, and about 50% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 3% to about 13% polyhexamethylene biguanide and from about 40% to about 60% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide and from about 42% to about 58% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide and from about 45% to about 55% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide and from about 47% to about 53% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 8% polyhexamethylene biguanide and about 50% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 3% to about 13% of a compound containing a biguanide moiety and from about 35% to about 55% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 5% to about 11% of a compound containing a biguanide moiety and from about 37% to about 53% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% of the compound containing the biguanide fragment, and from about 40% to about 50% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% of the compound containing the biguanide fragment, and from about 42% to about 48% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% of the compound containing the biguanide fragment, and about 45% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the implementation of the composition described in this application contains from about 3% to about 13% polyhexamethylene biguanide and from about 35% to about 55% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide and from about 37% to about 53% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide and from about 40% to about 50% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide and from about 42% to about 48% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains approximately 8%

- 47 042144 polyhexamethylene biguanide and about 45% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 3% to about 13% of a compound containing a biguanide moiety and from about 30% to about 50% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 5% to about 11% of a compound containing a biguanide moiety and from about 32% to about 48% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% of the compound containing the biguanide fragment, and from about 35% to about 45% of one or more alcohols. In other aspects, according to the specified embodiment, the composition described in this application contains from about 7% to about 9% of the compound containing the biguanide fragment, and from about 37% to about 43% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% of the compound containing the biguanide fragment, and about 40% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 3% to about 13% polyhexamethylene biguanide and from about 30% to about 50% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide and from about 32% to about 48% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide and from about 35% to about 45% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide and from about 37% to about 43% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 8% polyhexamethylene biguanide and about 40% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 3% to about 13% of a compound containing a biguanide moiety and from about 25% to about 45% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 5% to about 11% of a compound containing a biguanide moiety and from about 27% to about 43% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% of a compound containing a biguanide fragment, and from about 30% to about 40% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% of the compound containing the biguanide fragment, and from about 32% to about 38% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% of the compound containing the biguanide fragment, and about 35% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 3% to about 13% polyhexamethylene biguanide and from about 25% to about 45% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide and from about 27% to about 43% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide and

- 48 042144 from about 30% to about 40% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide and from about 32% to about 38% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 8% polyhexamethylene biguanide and about 35% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 6% to about 15% of a compound containing a biguanide moiety and from about 60% to about 80% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 7% to about 13% of a compound containing a biguanide moiety and from about 62% to about 78% of one or more alcohols. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% of the compound containing the biguanide fragment, and from about 65% to about 75% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of the compound containing the biguanide fragment, and from about 67% to about 73% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 10% of the compound containing the biguanide fragment, and about 70% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 6% to about 15% polyhexamethylene biguanide and from about 60% to about 80% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 7% to about 13% polyhexamethylene biguanide and from about 62% to about 78% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide and from about 65% to about 75% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide and from about 67% to about 73% isopropanol. In other aspects, according to the specified implementation variant, the composition described in this application contains about 10% polyhexamethylene biguanide and about 70% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 6% to about 15% of a compound containing a biguanide moiety and from about 55% to about 75% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 7% to about 13% of a compound containing a biguanide moiety and from about 57% to about 73% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% of the compound containing the biguanide fragment, and from about 60% to about 70% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of the compound containing the biguanide fragment, and from about 62% to about 68% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 10% of a compound containing a biguanide fragment, and about 65% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 6% to about 15% polyhexamethylene biguanide and from about 55% to about 75% isopropano

- 49 042144 la. In aspects according to the specified embodiment, the composition described in this application contains from about 7% to about 13% polyhexamethylene biguanide and from about 57% to about 73% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide and from about 60% to about 70% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide and from about 62% to about 68% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 10% polyhexamethylene biguanide and about 65% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 6% to about 15% of a compound containing a biguanide moiety and from about 50% to about 70% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 7% to about 13% of a compound containing a biguanide moiety and from about 52% to about 68% of one or more alcohols. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% of a compound containing a biguanide fragment, and from about 55% to about 65% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of the compound containing the biguanide fragment, and from about 57% to about 63% of one or more alcohols. In other aspects, according to the specified variant implementation of the composition described in this application contains about 10% of the compound containing the biguanide fragment, and about 60% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 6% to about 15% polyhexamethylene biguanide and from about 60% to about 70% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 7% to about 13% polyhexamethylene biguanide and from about 52% to about 68% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide and from about 55% to about 65% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide and from about 57% to about 63% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 10% polyhexamethylene biguanide and about 60% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 6% to about 15% of a compound containing a biguanide moiety and from about 45% to about 65% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 7% to about 13% of a compound containing a biguanide moiety and from about 47% to about 63% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% of the compound containing the biguanide fragment, and from about 50% to about 60% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of the compound containing the biguanide fragment, and from about 52% to about 58% of one or more alcohols. In other aspects, according to the specified variant implementation of the composition described in this application contains about 10% of the compound containing the biguanide fragment, and about 55% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used for eye

- 50 042144 stitches, disinfection and/or sterilization of the device or hard surface, as described in this application.

In one embodiment, the implementation of the composition described in this application contains from about 6% to about 15% polyhexamethylene biguanide and from about 45% to about 65% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 7% to about 13% polyhexamethylene biguanide and from about 47% to about 63% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide and from about 50% to about 60% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide and from about 52% to about 58% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 10% polyhexamethylene biguanide and about 55% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 6% to about 15% of a compound containing a biguanide moiety and from about 40% to about 60% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 7% to about 13% of a compound containing a biguanide moiety and from about 42% to about 58% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% of the compound containing the biguanide fragment, and from about 45% to about 55% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of the compound containing the biguanide fragment, and from about 47% to about 53% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 10% of the compound containing the biguanide fragment, and about 50% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 6% to about 15% polyhexamethylene biguanide and from about 40% to about 60% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 7% to about 13% polyhexamethylene biguanide and from about 42% to about 58% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide and from about 45% to about 55% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide and from about 47% to about 53% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 10% polyhexamethylene biguanide and about 50% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 6% to about 15% of a compound containing a biguanide moiety and from about 35% to about 55% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 7% to about 13% of a compound containing a biguanide moiety and from about 37% to about 53% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% of the compound containing the biguanide fragment, and from about 40% to about 50% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of the compound containing the biguanide fragment, and from about 42% to about 48% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application,

- 51 042144 contains about 10% of a compound containing a biguanide moiety and about 45% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 6% to about 15% polyhexamethylene biguanide and from about 35% to about 55% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 7% to about 13% polyhexamethylene biguanide and from about 37% to about 53% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide and from about 40% to about 50% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide and from about 42% to about 48% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 10% polyhexamethylene biguanide and about 45% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 6% to about 15% of a compound containing a biguanide moiety and from about 30% to about 50% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 7% to about 13% of a compound containing a biguanide moiety and from about 32% to about 48% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% of the compound containing the biguanide fragment, and from about 35% to about 45% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of the compound containing the biguanide fragment, and from about 37% to about 43% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 10% of a compound containing a biguanide fragment, and about 40% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 6% to about 15% polyhexamethylene biguanide and from about 30% to about 50% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 7% to about 13% polyhexamethylene biguanide and from about 32% to about 48% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide and from about 35% to about 45% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide and from about 37% to about 43% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 10% polyhexamethylene biguanide and about 40% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 6% to about 15% of a compound containing a biguanide moiety and from about 25% to about 45% of one or more alcohols. In aspects of this embodiment, the composition described herein contains from about 7% to about 13% of a compound containing a biguanide moiety and from about 27% to about 43% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% of a compound containing a biguanide fragment, and from about 30% to

- 52 042144 about 40% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of the compound containing the biguanide fragment, and from about 32% to about 38% of one or more alcohols. In other aspects, according to the specified implementation variant, the composition described in this application contains about 10% of the compound containing the biguanide fragment, and about 35% of one or more alcohols. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 6% to about 15% polyhexamethylene biguanide and from about 25% to about 45% isopropanol. In aspects according to the specified embodiment, the composition described in this application contains from about 7% to about 13% polyhexamethylene biguanide and from about 27% to about 43% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide and from about 30% to about 40% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide and from about 32% to about 38% isopropanol. In other aspects, according to the specified embodiment, the composition described in this application contains about 10% polyhexamethylene biguanide and about 35% isopropanol. In other aspects, in this embodiment, isopropanol may be replaced with a 5:1-1:5 mixture of isopropanol:ethanol. In other aspects, according to the specified variant implementation of the composition additionally contain from about 2% to about 7% cetylpyridinium chloride. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 6% to about 15% of a compound containing a biguanide moiety. In aspects according to the specified implementation variant, the composition described in this application contains from about 7% to about 13% of the compound containing the biguanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% of the compound containing the biguanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% of the compound containing the biguanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains about 10% of the compound containing the biguanide fragment. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 6% to about 15% polyhexamethylene biguanide. In aspects according to the specified embodiment, the composition described in this application contains from about 7% to about 13% polyhexamethylene biguanide. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 8% to about 12% polyhexamethylene biguanide. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 9% to about 11% polyhexamethylene biguanide. In other aspects, according to the specified implementation variant, the composition described in this application contains about 10% polyhexamethylene biguanide. In other aspects, according to said embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 3% to about 13% of a compound containing a biguanide moiety. In aspects according to the specified implementation variant, the composition described in this application contains from about 5% to about 11% of the compound containing the biguanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% of the compound containing the biguanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% of the compound containing the biguanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% of the compound containing the biguanide fragment. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a surfactant based on

- 53 042144 Quaternary ammonium. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 3% to about 13% polyhexamethylene biguanide. In aspects according to the specified implementation variant, the composition described in this application contains from about 5% to about 11% polyhexamethylene biguanide. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 6% to about 10% polyhexamethylene biguanide. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 7% to about 9% polyhexamethylene biguanide. In other aspects, according to the specified implementation variant, the composition described in this application contains about 8% polyhexamethylene biguanide. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

In one embodiment, the composition described herein contains from about 1% to about 10% of a compound containing a biguanide moiety. In aspects according to the specified implementation variant, the composition described in this application contains from about 3% to about 9% of the compound containing the biguanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 4% to about 8% of the compound containing the biguanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains from about 5% to about 7% of the compound containing the biguanide fragment. In other aspects, according to the specified implementation variant, the composition described in this application contains about 6% of the compound containing the biguanide fragment. In other aspects, according to the specified embodiment, the compositions further comprise from about 2% to about 7% of a quaternary ammonium surfactant. In other aspects, according to the specified embodiment, the compositions are used to clean, disinfect and/or sterilize a device or hard surface, as described in this application.

In one embodiment, the composition described in this application contains from about 1% to about 10% polyhexamethylene biguanide. In aspects according to the specified embodiment, the composition described in this application contains from about 3% to about 9% polyhexamethylene biguanide. In other aspects, according to the specified embodiment, the composition described in this application contains from about 4% to about 8% polyhexamethylene biguanide. In other aspects, according to the specified embodiment, the composition described in this application contains from about 5% to about 7% polyhexamethylene biguanide. In other aspects, according to the specified implementation variant, the composition described in this application contains about 6% polyhexamethylene biguanide. In other aspects, in this embodiment, the compositions are used to clean, disinfect, and/or sterilize an endoscope, an ECMO heart-lung machine, or a hard surface, as described herein.

A kit is described in aspects according to the present invention. In one embodiment, the kit may include a container containing the composition described in this application. In another implementation, the kit may include one or more containers, with each of the containers containing a single component or a combination of more than one of the individual components described in this application. For example, the kit may include a first container containing hypochlorous acid or free active chlorine and a second container containing one or more disinfectants. As another example, the kit may include a first container containing one or more alcohols and a second container containing one or more disinfectants. As another example, the kit may include a first container containing isopropanol and a second container containing polyhexamethylene biguanide. As another example, the kit may include a first container containing a mixture of isopropanol and ethanol, and a second container containing polyhexamethylene biguanide. As another example, the kit may include a first container containing isopropanol, a second container containing ethanol, and a third container containing polyhexamethylene biguanide. As another example, the kit may include a first container containing isopropanol, a second container containing polyhexamethylene biguanide, and a third container containing one or more cationic surfactants. The remaining components of the composition described in this application may be contained in the first or second container, or may be separately contained in at least a third container. For example, the kit may include a third container containing the wash solution described in this application. Packing the individual components in a separate container can help extend the stability of the individual components and thus increase the shelf life of the product.

In another example, the kit may include a first container containing hypochlorous acid and/or free active chlorine, a second container containing one or more disinfectant agents.

- 54 042144 tov, and a third container containing a solution for washing. As another example, the kit may include a first container containing one or more alcohols, a second container containing one or more disinfectants, and a third container containing a wash solution. As another example, the kit may include a first container containing isopropanol, a second container containing polyhexamethylene biguanide, and a third container containing a wash solution. As another example, the kit may include a first container containing a mixture of isopropanol and ethanol, a second container containing polyhexamethylene biguanide, and a third container containing a wash solution. As another example, the kit may include a first container containing isopropanol, a second container containing ethanol, a third container containing polyhexamethylene biguanide, and a fourth container containing a wash solution. As another example, the kit may include a first container containing isopropanol, a second container containing polyhexamethylene biguanide, a third container containing one or more cationic surfactants, and a fourth container containing a wash solution.

In another embodiment, the kit may include a first container containing the composition described in this application, and a second container containing the washing solution described in this application. For example, the kit may include a first container containing hypochlorous acid and/or free active chlorine and one or more disinfecting agents, and a second container containing a wash solution. As another example, the kit may include a first container containing one or more alcohols and one or more disinfectants, and a second container containing a wash solution. As another example, the kit may include a first container containing isopropanol and polyhexamethylene biguanide and a second container containing a wash solution. As another example, the kit may include a first container containing a mixture of isopropanol and ethanol and polyhexamethylene biguanide, and a second container containing a wash solution. As another example, the kit may include a first container containing isopropanol, polyhexamethylene biguanide and one or more cationic surfactants, and a second container containing a wash solution. The remaining components of the composition described in this application may be contained in the first container or may be separately contained in at least a third container. For example, the kit may include a third container containing the wash solution described in this application. Packing the individual components in a separate container can help extend the stability of the individual components and thus increase the shelf life of the product.

The set described in this application may include a delivery or application system. A kit delivery or application system is useful for applying the composition described herein and/or the individual components described herein to an area of interest, such as, for example, the surface of a device described herein. The delivery or application system described herein includes, without limitation, one or more applicator brushes, foam swabs or wipes, hollow tubes, test strips, or a combination thereof. In one embodiment, the kit includes one delivery or application system. In another embodiment, the kit includes a plurality of delivery or application systems. For example, a 30 day kit may include 30 delivery or application systems, such that one delivery or application system is provided for each of the 30 days. Alternatively, the set may include 2, 10, 20, 30, 40, 50, 60, 90, 120, etc. delivery or application systems. The included delivery or application systems may be packaged individually or in sets of 2 or more. The delivery or application system may be packaged such that it remains sterile until use. In some embodiments, the delivery or application system described herein may be packaged in plastic containers. In addition, to prevent contamination, the delivery or application system described in this application is preferably a disposable delivery or application system.

The kit may also include a set of instructions. The instructions may include information useful to the end user, such as a description of the use of the delivery or application system to apply the composition and/or individual components described in this application, and/or a description of the frequency of application of the composition and/or individual components described in this application. In addition, such instructions may include information on how to mix the individual components described in this application to obtain the composition described in this application. Such instructions may indicate that the mixing should be carried out at a certain time before application, such as, for example, immediately before use. The instructions described in this application may also include information on how to apply the individual components described in this application directly to the area of interest, such as, for example, the surface of the device described in this application, and in what order the individual components should be applied. to such areas of interest.

The contents of the kit, including a container containing the composition or component described in this application, the delivery or application system and instructions, is placed in the outer casing. External

- 55 042144 the body can be a box, a sealed bag, a foil sachet, etc. In some embodiments, the delivery system, container, and instructions are placed in a box. In other embodiments of the kit, the container and instructions are placed in a first box, the delivery system is placed in a second box, and the first and second boxes are placed together in a third box.

In aspects according to the present invention, a method for cleaning, disinfecting and/or sterilizing a device, including a medical device, is described. In one aspect, according to said embodiment, the method described herein comprises applying the composition described herein to a device for a specified period of time, wherein the application results in cleaning, disinfection, and/or sterilization of the device. In another aspect, according to said embodiment, the method described herein further comprises rinsing the cleaned, disinfected, and/or sterilized device with a wash solution described herein. In other aspects, according to the present invention, a composition described in this application is described for use in cleaning, disinfecting and/or sterilizing a device, including a medical device. In other aspects, according to the present invention, the use of the described composition for cleaning, disinfecting and/or sterilizing a device, including a medical device, is described.

In aspects according to the present invention, a method for cleaning, disinfecting and/or sterilizing an endoscope is described. In one aspect, according to the specified embodiment, the method described in this application includes applying the composition described in this application to the endoscope for a certain period of time, while the application leads to cleaning, disinfection and / or sterilization of the endoscope. In another aspect, according to said embodiment, the method described herein further comprises rinsing the cleaned, disinfected, and/or sterilized endoscope with the wash solution described herein. In other aspects, according to the present invention, a composition described in this application is described for use in cleaning, disinfecting and/or sterilizing an endoscope. In other aspects, according to the present invention, the use of the described composition for cleaning, disinfecting and/or sterilizing an endoscope is described.

A device, including a medical device, is cleaned by removing visible contaminants, such as, for example, organic and inorganic material, from objects and surfaces, and cleaning is usually carried out manually or mechanically. Thorough cleaning is necessary prior to disinfection and sterilization, as inorganic and organic materials that remain on the surfaces of a medical device impair the effectiveness of these processes.

A device, including a medical device, is disinfected by killing many or all of the pathogens except for bacterial spores. Disinfection is less destructive than sterilization because it kills most recognized pathogens, but not necessarily all microbial forms (eg, bacterial spores). A medical device is sterilized by destroying or eliminating all forms of microorganisms. Decontamination of a medical device removes pathogenic microorganisms from a medical device so that it can be safely used, used, or disposed of.

A medical device is an instrument, apparatus, material or other product that can be used individually or in combination, including the software necessary for its use, intended by the manufacturer for use in humans to diagnose, prevent, control, treat or alleviate a disease; diagnosing, monitoring, treating or alleviating an injury or defect or compensating for the consequences of an injury or defect; research, replacement or modification of the anatomy or physiological process; or control of conception, and for processes that do not achieve their primary intended action in or on the human body by pharmacological, immunological or metabolic means, but can be assisted in their functioning by such means. The medical device includes, without limitation, a surgical instrument, a respiratory therapy instrument, an anesthesia instrument, a catheter, an implant, a probe, an endoscope, an arthroscope, a laparoscope, a blade, a cystoscope, a spirometer, a CPAP mask and tubing, a dialysis instrument and accessories. , ECMO heart-lung machine and accessories, heart-lung machine and accessories, and diaphragm adjustment ring. Non-limiting examples of probes include an ultrasound probe and an esophageal manometry probe. Non-limiting examples of catheters include cardiac catheter, urinary catheter, anorectal manometry catheter. Non-limiting examples of endoscopes include a gastrointestinal endoscope, a bronchoscope, and a nasopharyngoscope. Non-limiting examples of blades include a laryngoscope blade.

Aspects of the present invention describe a method for cleaning, disinfecting and/or sterilizing a hard surface area. In one aspect, according to said embodiment, the method described herein comprises applying the composition described herein to a hard surface area for a specified period of time, wherein the application results in cleaning, disinfecting, and/or sterilizing the hard surface area. In another aspect, with

- 56 042144 According to the specified implementation variant, the method described in this application further includes rinsing the cleaned, disinfected and/or sterilized surface with a washing solution described in this application. In other aspects, according to the present invention, a composition described in this application is described for use in cleaning, disinfecting and/or sterilizing a hard surface area. In other aspects, according to the present invention, the use of the described composition for cleaning, disinfecting and/or sterilizing a hard surface area is described. The hard surface region may be a porous surface region or a non-porous surface region.

In another aspect, according to said embodiment, the method described herein comprises applying one or more of the individual components described herein to a hard surface area for a specified period of time, wherein the application results in cleaning, disinfecting, and/or sterilizing the hard surface area. surfaces. In one aspect, according to said embodiment, the one or more individual components comprise a first component containing hypochlorous acid or free active chlorine and a second component containing one or more disinfectants. In another aspect, the application of one or more individual components occurs in a certain order, such as, for example, the first component containing hypochlorous acid or free active chlorine is applied first, and then the second component containing one or more disinfecting agents is applied. The hard surface region may be a porous surface region or a non-porous surface region.

The hard surface area may include any objects present in a residential or commercial, industrial and/or agricultural establishment such as, for example, a hospital, laboratory, restaurant, training center, food processing plant, dairy processing plant, airport, oil field system, sports complex, shipping port, freight center and any other commercial or industrial facility. The surface area may include any type of vehicle such as, for example, a watercraft such as a boat, barge or ship, an aircraft such as an airplane or helicopter, a land vehicle such as a motorcycle, car, truck or train. The surface area can be made from any material including brass, copper, aluminum, stainless steel, carbon steel, rubber, plastic, glass, wood, painted surface, or any combination thereof. Surface area includes, but is not limited to, tabletop, countertop, floor, wall, ceiling, window, bed, wheelchair, door, doorknob, shower, bathtub, sink, faucet, toilet, toilet seat, sink, hardware, appliances, facilities personal protective equipment, personal biological protection equipment, etc. The surface area may include a medical, dental, pharmaceutical, veterinary, or mortuary device. The surface area may include human skin.

The composition or component described in this application can be applied to a hard surface area in accordance with the method described in this application, as often as necessary and/or desirable. The composition described in this application can be applied to a hard surface area daily, every other day, every two days, once a week, several times a week, once a month, several times a month, once a year or several times a year, according to desire. The composition described in this application can be applied to the hard surface area several times a day, for example, twice a day, three times a day, four times a day, five times a day, six times a day, or as often as desirable.

In aspects of the present invention, a method for cleaning, disinfecting and/or sterilizing a microbial infection in a patient is described. In one aspect, according to said embodiment, the method described herein comprises applying the composition described herein to a patient for a period of time, wherein the application results in the purification, disinfection, and/or sterilization of a microbial infection in the patient. In other aspects, according to the present invention, a composition described in this application is described for use in cleaning, disinfecting and/or sterilizing a microbial infection in a patient. In other aspects, according to the present invention, the use of the described composition for cleaning, disinfecting and/or sterilizing a microbial infection in a patient is described. In other aspects, according to the present invention, a composition described in this application is described for the manufacture of a medicinal product for cleaning, disinfecting and/or sterilizing a microbial infection in a patient.

The method and/or use described in this application cleans, disinfects and/or sterilizes a microbial infection. Microbial infection includes viral infection, bacterial infection and fungal infection.

In the method and/or application described in this application, the composition described in this application is applied to the patient. The patient is any animal, including, without limitation, a fish, amphibian, bird, or mammal. Mammal includes human, horse, cow, sheep, dog and cat. Thus, the method described in this application is intended for use in humans as well as for veterinary use.

- 57 042144

When applied to a patient, the composition described in this application can be applied to the surface of the skin or can be administered orally. In one embodiment, the composition described herein is applied topically to a patient's skin area to clean, disinfect, and/or sterilize the skin area. The skin area includes, without limitation, the face, forehead, lips, scalp, neck, shoulders, arms, hands, thighs, legs, knees, feet, chest, chest, back, groin, buttocks, and the like.

In one embodiment, the composition described in this application is intended for internal use by a patient. Such routes of administration include enteral routes of administration and parenteral routes of administration.

The composition described in this application can be applied in accordance with the method described in this application to the area of the skin. The application of the composition described in this application can be carried out by rubbing, pouring, spraying or spraying or otherwise applied to the human body. The composition described in this application can be applied by introducing the composition into a hard support or on a hard support, such as, for example, a wipe, wet wipe, towel, mitt, glove or mask, and then applying the composition to the area of the skin. The composition described herein can be applied using a delivery device, such as, for example, an aerosol dispenser, atomizer, trigger, squeeze bottle, topical patch, transdermal patch, or skin implant, to apply the composition to a skin area.

The composition described in this application can be applied to the patient in accordance with the method described in this application, as often as necessary and/or desirable. The composition described in this application can be applied to the patient daily, every other day, every other day, once a week, several times a week, once a month, several times a month, once a year or several times a year, as desired. The composition described in this application can be applied to the patient several times a day, for example, twice a day, three times a day, four times a day, five times a day, six times a day, or as often as desired.

In the described method and/or application, the composition described in this application is applied for a certain period of time. In one embodiment, the defined period of time is sufficient time to clean the medical device, surface, or patient. In another embodiment, the defined period of time is the time sufficient to disinfect the medical device, surface, or patient. In another implementation, a certain period of time is a time sufficient to sterilize a medical device, surface or patient.

In aspects of said embodiment, the composition described herein is applied to a device, such as a medical device, surface, or applied to a patient, for a period of time, such as about 1 minute, about 5 minutes, about 10 minutes, about 15 minutes, approximately 20 minutes, approximately 25 minutes, approximately 30 minutes, approximately 35 minutes, approximately 40 minutes, approximately 45 minutes, approximately 50 minutes, approximately 55 minutes, approximately 60 minutes, approximately 70 minutes, approximately 80 minutes, approximately 90 minutes, about 100 minutes, about 110 minutes, or about 120 minutes. In other aspects, according to the specified embodiment, the composition described in this application is applied to a device, such as a medical device, a surface, or applied to a patient for a period of time, for example, at least 1 minute, at least 5 minutes, at least 10 minutes, at least 15 minutes, at least 20 minutes, at least 25 minutes, at least 30 minutes, at least 35 minutes, at least 40 minutes, at least 45 minutes, at least 50 minutes, at least 55 minutes, at least 60 minutes, at least 70 minutes, at least 80 minutes, at least 90 minutes, at least 100 minutes, at least 110 minutes, or at least 120 minutes . In other aspects, according to the specified embodiment, the composition described in this application is applied to a device, such as a medical device, surface, or applied to a patient for a period of time, for example, no more than 1 minute, no more than 5 minutes, no more than 10 minutes, no more than 15 minutes, no more than 20 minutes, no more than 25 minutes, no more than 30 minutes, no more than 35 minutes, no more than 40 minutes, no more than 45 minutes, no more than 50 minutes, no more than 55 minutes, no more than 60 minutes, no more than 70 minutes, no more than 80 minutes, no more than 90 minutes, no more than 100 minutes, no more than 110 minutes, or no more than 120 minutes.

In other aspects, according to the specified embodiment, the composition described in this application is applied to a device, such as a medical device, surface, or applied to a patient for a period of time, for example, from about 1 minute to about 5 minutes, from about 1 minute up to about 10 minutes, from about 1 minute to about 15 minutes, from about 1 minute to about 20 minutes, from about 1 minute to about 25 minutes, from about 1 minute to about 30 minutes, from about 1 minute to about 35 minutes, from about 1 minute to about 40 minutes, from about 1 minute to about 45 minutes, from about 1 minute to about 50 minutes, from about 1 minute to about 55 minutes, from about 1 minute to about 60 minutes, from about 5 minutes to about 10 minutes, from about 5 minutes to about 15 minutes, from about 5

- 58 042144 minutes to about 20 minutes, from about 5 minutes to about 25 minutes, from about 5 minutes to about 30 minutes, from about 5 minutes to about 35 minutes, from about 5 minutes to about 40 minutes, from about 5 minutes to about 45 minutes, from about 5 minutes to about 50 minutes, from about 5 minutes to about 55 minutes, from about 5 minutes to about 60 minutes, from about 5 minutes to about 70 minutes, from about 5 minutes to about 80 minutes, from about 5 minutes to about 90 minutes, from about 5 minutes to about 100 minutes, from about 5 minutes to about 110 minutes, from about 5 minutes to about 120 minutes, from about 10 minutes to about 15 minutes, from about 10 minutes to about 20 minutes, from about 10 minutes to about 25 minutes, from about 10 minutes to about 30 minutes, from about 10 minutes to about 35 minutes, from about 10 minutes to about 40 minutes, from about 10 minutes to about 45 minutes, from about 10 minutes to about 50 minutes, from about 10 minutes to n about 55 minutes, from about 10 minutes to about 60 minutes, from about 10 minutes to about 70 minutes, from about 10 minutes to about 80 minutes, from about 10 minutes to about 90 minutes, from about 10 minutes to about 100 minutes, from about 10 minutes to about 110 minutes, from about 10 minutes to about 120 minutes, from about 15 minutes to about 20 minutes, from about 15 minutes to about 25 minutes, from about 15 minutes to about 30 minutes, from about 15 minutes to about 35 minutes, from about 15 minutes to about 40 minutes, from about 15 minutes to about 45 minutes, from about 15 minutes to about 50 minutes, from about 15 minutes to about 55 minutes, from about 15 minutes to about 60 minutes, from about 15 minutes to about 70 minutes, from about 15 minutes to about 80 minutes, from about 15 minutes to about 90 minutes, from about 15 minutes to about 100 minutes, from about 15 minutes to about 110 minutes, from about 15 minutes to about 120 minutes, from about 20 minutes to but 25 minutes, from about 20 minutes to about 30 minutes, from about 20 minutes to about 35 minutes, from about 20 minutes to about 40 minutes, from about 20 minutes to about 45 minutes, from about 20 minutes to about 50 minutes, from about 20 minutes to about 55 minutes, from about 20 minutes to about 60 minutes, from about 20 minutes to about 70 minutes, from about 20 minutes to about 80 minutes, from about 20 minutes to about 90 minutes, from about 20 minutes to about 100 minutes, from about 20 minutes to about 110 minutes, from about 20 minutes to about 120 minutes, from about 25 minutes to about 30 minutes, from about 25 minutes to about 35 minutes, from about 25 minutes to about 40 minutes, from about 25 minutes to about 45 minutes, from about 25 minutes to about 50 minutes, from about 25 minutes to about 55 minutes, from about 25 minutes to about 60 minutes, from about 25 minutes to about 70 minutes, from about 25 minutes to about 80 minutes, from about 25 minutes to about 90 minutes chickpeas, from about 25 minutes to about 100 minutes, from about 25 minutes to about 110 minutes, from about 25 minutes to about 120 minutes, from about 30 minutes to about 35 minutes, from about 30 minutes to about 40 minutes, from about 30 minutes to about 45 minutes, from about 30 minutes to about 50 minutes, from about 30 minutes to about 55 minutes, from about 30 minutes to about 60 minutes, from about 30 minutes to about 70 minutes, from about 30 minutes to about 80 minutes , from about 30 minutes to about 90 minutes, from about 30 minutes to about 100 minutes, from about 30 minutes to about 110 minutes, from about 30 minutes to about 120 minutes, from about 35 minutes to about 40 minutes, from about 35 minutes up to about 45 minutes, from about 35 minutes to about 50 minutes, from about 35 minutes to about 55 minutes, from about 35 minutes to about 60 minutes, from about 35 minutes to about 70 minutes, from about 35 minutes to about 80 minutes, from about 35 minutes to about 90 minutes, about 35 minutes to about 100 minutes, from about 35 minutes to about 110 minutes, from about 35 minutes to about 120 minutes, from about 40 minutes to about 45 minutes, from about 40 minutes to about 50 minutes, from about 40 minutes to about 55 minutes, from about 40 minutes to about 60 minutes, from about 40 minutes to about 70 minutes, from about 40 minutes to about 80 minutes, from about 40 minutes to about 90 minutes, from about 40 minutes to about 100 minutes, from about 40 minutes to about 110 minutes, from about 40 minutes to about 120 minutes, from about 45 minutes to about 50 minutes, from about 45 minutes to about 55 minutes, from about 45 minutes to about 60 minutes, from about 45 minutes to about 70 minutes, from about 45 minutes to about 80 minutes, from about 45 minutes to about 90 minutes, from about 45 minutes to about 100 minutes, from about 45 minutes to about 110 minutes, from about 45 minutes to about 120 minutes, from about 50 minutes to about 55 minutes, from about 50 minutes to about 60 minutes, from about 50 minutes to about 70 minutes, from about 50 minutes to about 80 minutes, from about 50 minutes to about 90 minutes, from about 50 minutes to about 100 minutes, from about 50 minutes to about 110 minutes, from about 50 minutes to about 120 minutes, from about 55 minutes to about 60 minutes, from about 55 minutes to about 70 minutes, from about 55 minutes to about 80 minutes, from about 55 minutes to about 90 minutes, from about 55 minutes to about 100 minutes, from about 55 minutes to about 110 minutes, from about 55 minutes to about 120 minutes, from about 60 minutes to about 70 minutes, from about 60 minutes to about 80 minutes, from about 60 minutes to about 90 minutes, from about 60 minutes to about 100 minutes, from about 60 minutes to about 110 minutes, from about 60 minutes to about 120 minutes, from about 70 minutes to about 80 minutes, from about 70 minutes to about 90 minutes, from about 70 minutes to about 100 minutes, from about 70 minutes to about 110 mi

- 59 042144 chickpeas, from about 70 minutes to about 120 minutes, from about 80 minutes to about 90 minutes, from about 80 minutes to about 100 minutes, from about 80 minutes to about 110 minutes, from about 80 minutes to about 120 minutes, from about 90 minutes to about 100 minutes, from about 90 minutes to about 110 minutes, from about 90 minutes to about 120 minutes, from about 100 minutes to about 110 minutes, from about 100 minutes to about 120 minutes, or from about 110 minutes to approximately 120 minutes.

The described composition, method, and/or use is less damaging to the medical device, resulting in a longer service life of the medical device. In aspects of this embodiment, the medical device may be cleaned, disinfected, and/or sterilized, for example, about 50 times, about 60 times, about 70 times, about 80 times, about 90 times, about 100 times, about 110 times, about 120 times. , about 130 times, about 140 times, about 150 times, about 160 times, about 170 times, about 180 times, about 190 times, about 200 times, about 210 times, about 220 times, about 230 times, about 240 times, about 250 times, about 260 times, about 270 times, about 280 times, about 290 times, or about 300 times. In other aspects, according to the specified embodiment, the medical device can be cleaned, disinfected and/or sterilized, for example, at least 50 times, at least 60 times, at least 70 times, at least 80 times, at least 90 times, at least 100 times, at least 110 times, at least 120 times, at least 130 times, at least 140 times, at least 150 times, at least 160 times, at least 170 times, at least at least 180 times, at least 190 times, at least 200 times, at least 210 times, at least 220 times, at least 230 times, at least 240 times, at least 250 times, at least 260 times times, at least 270 times, at least 280 times, at least 290 times, or at least 300 times. In other aspects, according to the specified embodiment, the medical device can be cleaned, disinfected and/or sterilized, for example, no more than 50 times, no more than 60 times, no more than 70 times, no more than 80 times, no more than 90 times, no more than 100 times, no more than 110 times, no more than 120 times, no more than 130 times, no more than 140 times, no more than 150 times, no more than 160 times, no more than 170 times, no more than 180 times, no more than 190 times, no more than 200 times, no more than 210 times, no more than 220 times, no more than 230 times, no more than 240 times, no more than 250 times, no more than 260 times, no more than 270 times, no more than 280 times, no more than 290 times or no more than 300 times.

In other aspects, according to the specified embodiment, the medical device can be cleaned, disinfected and/or sterilized, for example, from about 50 times to about 60 times, from about 50 times to about 70 times, from about 50 times to about 80 times, from about 50 times to about 90 times, from about 50 times to about 100 times, from about 50 times to about 110 times, from about 50 times to about 120 times, from about 50 times to about 130 times, from about 50 times to about 140 times , from about 50 times to about 150 times, from about 50 times to about 175 times, from about 50 times to about 200 times, from about 50 times to about 225 times, from about 50 times to about 250 times, from about 50 times up to about 275 times, from about 50 times to about 300 times, from about 75 times to about 90 times, from about 75 times to about 100 times, from about 75 times to about 110 times, from about 75 times to about 120 times, from about 75 times to about 130 times, from about 75 times to about 140 times, from about 75 times to about 150 times, from about 75 times to about 175 times, from about 75 times to about 200 times, from about 75 times to about 225 times, from about 75 times to about 250 times, from about 75 times to about 275 times, from about 75 times to about 300 times, from about 100 times to about 110 times, from about 100 times to about 120 times, from about 100 times to about 130 times, from about 100 times to about 140 times, from about 100 times to about 150 times, from about 100 times to about 175 times, from about 100 times to about 200 times, from about 100 times to about 225 times, from about 100 times to about 250 times, from about 100 times to about 275 times, about 100 times to about 300 times, from about 125 times to about 150 times, from about 125 times to about 175 times, from about 125 times to about 200 times, from about 125 times to about 225 times times, from about 125 times to about 250 times, from about 125 times to about 275 times, from about 125 times to about 300 times, from about 150 times to about 175 times, from about 150 times to about 200 times, from about 150 times to about 225 times, from about 150 times to about 250 times, from about 150 times to about 275 times, from about 150 times to about 300 times, from about 175 times to about 200 times, from about 175 times to about 225 times, from about 175 times to about 250 times, from about 175 times to about 275 times, from about 175 times to about 300 times, about 200 times to about 225 times, from about 200 times to about 250 times, from about 200 times to about 275 times, from about 200 times to about 300 times, from about 225 times to about 250 times times, from about 225 times to about 275 times, from about 225 times to about 300 times, from about 250 times to about 275 times, from about 250 times to about 300 times, or from about 275 times to about 300 times.

The method described in this application may further include the step of washing using a washing solution. Typically, a wash solution is used to wash a cleaned, disinfected and/or sterilized medical device or surface. The washing solution is preferably a sterile solution. In one of the

- 60 042144 embodiments, the washing solution described in this application contains water. In another embodiment, the implementation of the solution for washing, described in this application contains hypochlorous acid or free active chlorine and water. In another embodiment, the implementation of the solution for washing, described in this application does not contain hypochlorous acid and/or free active chlorine. When present in the kit described in this application, the wash solution is contained in a separate container.

The amount of hypochlorous acid or free active chlorine contained in the wash solution described in this application is any amount that provides an antimicrobial effect, provided that the total amount of hypochlorous acid or free active chlorine contained is an amount that is less than the threshold level , which leads to the oxidation of a medical device or surface, as described in this application. In aspects of said embodiment, the amount of hypochlorous acid or free active chlorine contained in the wash solution may be, for example, about 5 ppm, about 10 ppm, about 20 ppm, about 30 ppm, about 40 ppm, about 50 ppm, about 60 ppm, about 70 ppm, about 80 ppm, about 90 ppm, about 100 ppm, about 110 ppm, or about 120 ppm. In other aspects, according to the specified embodiment, the amount of hypochlorous acid or free active chlorine contained in the wash solution may be, for example, no more than 5 ppm, no more than 10 ppm, no more than 20 ppm, no more than 30 ppm, no more than 40 ppm , 50ppm max, 60ppm max, 70ppm max, 80ppm max, 90ppm max, 100ppm max, 110ppm max, or 120ppm max. In other aspects, according to said embodiment, the amount of hypochlorous acid or free active chlorine contained in the wash solution may be, for example, from about 5 ppm to about 10 ppm, from about 5 ppm to about 20 ppm, from about 5 ppm to about 30 ppm, about 5 ppm to about 40 ppm, about 5 ppm to about 50 ppm, about 5 ppm to about 60 ppm, about 5 ppm to about 70 ppm, about 5 ppm to about 80 ppm, from about 5 ppm to about 90 ppm, about 5 ppm to about 100 ppm, about 5 ppm to about 110 ppm, about 5 ppm to about 120 ppm, about 10 ppm to about 20 ppm, about 10 ppm to about 30 ppm, from about 10 ppm to about 40 ppm, from about 10 ppm to about 50 ppm, from about 10 ppm to about 60 ppm, from about 10 ppm to about 70 ppm, from about 10 ppm to about 80 ppm, from about 10 ppm to about 90 ppm, from about 10 ppm to about 100 pp m, from about 10 ppm to about 110 ppm, from about 10 ppm to about 120 ppm, from about 20 ppm to about 30 ppm, from about 20 ppm to about 40 ppm, from about 20 ppm to about 50 ppm, from about 20 ppm to about 60 ppm, about 20 ppm to about 70 ppm, about 20 ppm to about 80 ppm, about 20 ppm to about 90 ppm, about 20 ppm to about 100 ppm, about 20 ppm to about 110 ppm , from about 20 ppm to about 120 ppm, from about 30 ppm to about 40 ppm, from about 30 ppm to about 50 ppm, from about 30 ppm to about 60 ppm, from about 30 ppm to about 70 ppm, from about 30 ppm up to about 80 ppm, from about 30 ppm to about 90 ppm, from about 30 ppm to about 100 ppm, from about 30 ppm to about 110 ppm, from about 30 ppm to about 120 ppm, from about 40 ppm to about 50 ppm, from about 40 ppm to about 60 ppm, from about 40 ppm to about 70 ppm, from about 40 ppm to about 80 ppm, from about 40 ppm to about 90 ppm, about 40 ppm to about 100 ppm, about 40 ppm to about 110 ppm, about 40 ppm to about 120 ppm, about 50 ppm to about 60 ppm, about 50 ppm to about 70 ppm , from about 50 ppm to about 80 ppm, from about 50 ppm to about 90 ppm, from about 50 ppm to about 100 ppm, from about 50 ppm to about 110 ppm, from about 50 ppm to about 120 ppm, from about 60 ppm up to about 70 ppm, from about 60 ppm to about 80 ppm, from about 60 ppm to about 90 ppm, from about 60 ppm to about 100 ppm, from about 60 ppm to about 110 ppm, from about 60 ppm to about 120 ppm, from about 70 ppm to about 80 ppm, from about 70 ppm to about 90 ppm, from about 70 ppm to about 100 ppm, from about 70 ppm to about 110 ppm, from about 70 ppm to about 120 ppm, from about 80 ppm to about 90 ppm, from about 80 ppm to about 100 ppm, from about 80 ppm to about 110 ppm, from about 80 ppm to at 120 ppm, about 90 ppm to about 100 ppm, about 90 ppm to about 110 ppm, about 90 ppm to about 120 ppm, about 100 ppm to about 110 ppm, about 100 ppm to about 120 ppm, or from about 110 ppm to about 120 ppm.

Examples

The following non-limiting examples are provided for illustrative purposes only to facilitate a more complete understanding of the considered embodiments given as examples. These examples should not be considered as limiting any of the implementation options described in the present invention, including implementation options that relate to the compositions, methods and uses described in this application.

Example 1. Compositions.

This example illustrates a method for obtaining the composition described in this application.

- 61 042144

The components listed below were mixed at ambient temperature using a high shear mixer until a homogeneous mixture was obtained. The pH of the mixture was adjusted as shown. For amounts of hypochlorous acid, unless otherwise indicated, the amount is the final concentration after mixing with other components.

Table 1 Compositions of compositions

Component F1 F2 F3 F4 F5 F6 Hypochlorous acid 84ppm 84ppm 84ppm 97ppm 97ppm 97ppm Polyhexamethylene biguanide — — — 0.25% 0.5% 1.0% Isopropyl alcohol — — — — — — Glutaraldehyde 0.1% 0.25% 0.5% — — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH 5.8 6.2 6.25 6.85 6.5 6.7

Table 2 Compositions of compositions

Component F7 F8 F9 F10 Fil F12 Hypochlorous acid 97ppm 97ppm 97ppm 97ppm 97ppm 97ppm Polyhexamethylene biguanide — — — — — — Isopropyl alcohol — — — — — — Glutaraldehyde 0.5% 0.5% 0.5% 0.25% 0.25% 0.25% Sodium lauryl sulfate 2% 2% 2% 2% 2% 2% Water q.s. q.s. q.s. q.s. q.s. q.s. pH 3 4 5 3.5 4.5 5.5

Table 3 Compositions of compositions

Component F13 F14 F15 F16 F17 F18 Hypochlorous acid 66ppm 76ppm 80ppm 82ppm 73ppm 75ppm Polyhexamethylene biguanide — — — — — — Isopropyl alcohol — — — — — — Glutaraldehyde 0.5% 0.5% 0.5% 0.25% 0.25% 0.25% Water q.s. q.s. q.s. q.s. q.s. q.s. pH 3 4 5 3.5 4.5 5.5

Table 4 Compositions of compositions

Component F19 F20 F21 F22 F23 F24 Hypochlorous acid 22ppm 18ppm 16ppm 11ppm — — Polyhexamethylene biguanide 1.25% 1.5% 1.75% 2.0% 2.0% 3.0% Isopropyl alcohol — — — — 70% 70% Glutaraldehyde — — — — — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH 5.75 6.6 6.4 6.9 H/O H/O

N/A, not defined.

Table 5 Compositions of compositions

Component F25 F26 F27 F28 F29 F30 Hypochlorous acid — — — — — — Polyhexamethylene biguanide 4.0% 6.0% 8.0% 10% 0.25% 0.5% Isopropyl alcohol 70% 70% 70% 70% 70% 70% Glutaraldehyde — — — — — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH BUT 6.3 6.4 6.4 H/O H/O

N/A, not defined.

- 62 042144

Table 6 Compositions of compositions

Component F31 F32 F33 F34 F35 F36 Hypochlorous acid — — — — — — Polyhexamethylene biguanide 0.75% 1.0% 1.25% 1.5% 1.75% 2.0% Isopropyl alcohol 70% 70% 70% 70% 70% 70% Glutaraldehyde — — — — — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH BUT BUT BUT BUT BUT BUT

N/A, not defined.

Table 7 Compositions of compositions

Component F37 F38 F39 F40 F41 F42 Hypochlorous acid 96ppm 94ppm 92ppm 90ppm 98ppm 96ppm Polyhexamethylene biguanide 4.0% 6.0% 8.0% 10.0% 2.0% 4.0% Isopropyl alcohol — — — — — — Glutaraldehyde — — — — — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH BUT BUT BUT H/O H/O H/O

N/A, not defined.

Table 8 Compositions of compositions

Component F43 F44 F45 F46 F47 F48 Hypochlorous acid 94ppm 92ppm 90ppm 46ppm 58ppm — Polyhexamethylene biguanide 6.0% 8.0% 10.0% 0.25% 0.25% 0.25% Isopropyl alcohol — — — — — 40% Glutaraldehyde — — — — — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH BUT BUT H/O 6.8 6.5 5.4

N/A, not defined.

Table 9 Compositions of compositions

Component F49 F50 F51 F52 F53 F54 Hypochlorous acid — — — — 83ppm 56ppm Polyhexamethylene biguanide 0.25% 0.01% 0.001% 0.05% — 0.01% Isopropyl alcohol 20% 70% 70% 70% — — Glutaraldehyde — — — — — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH 5.5 5.8 5.8 6.0 6.6 7.4

Table 10 Compositions of compositions

Component F55 F56 F57 F58 F59 F60 Hypochlorous acid — — — — — — Polyhexamethylene biguanide 8.0% 9.5% 5.0% 10.0% 10.0% 10.0% Isopropyl alcohol 70% 70% 70% 65% 60% 50% Glutaraldehyde 2.0% — — — — — opmo-ΦτΆΛ aldehyde — 0.5% — — — — Benzalkonium chloride — — 5.0% 5.0% — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH 4.0 3.9 7.7 7.3 6.0 6.0

N/A, not defined.

-63 042144

Table 11 Compositions of compositions

Component F61 F62 F63 F64 F65 F66 Hypochlorous acid — — — — — — Polyhexamethylene biguanide 10.0% 10.0% 10.0% 10.0% 1% 2% Isopropyl alcohol — — 25.0% 33.33% 50% 50% ethanol — 50.0% 25.0% 16.67% — — Glutaraldehyde — — — — — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH BUT 6.15 6.38 6.29 6.01 6.19

Table 12 Compositions of compositions

Component F67 F68 F69 F70 F71 F97 hypochlorous acid — — — — — 530ppm* Polyhexamethylene biguanide 4.0% 6.0% 8.0% 10.0% 10.0% 500ppm Isopropyl alcohol 50.0% — 50.0% 40% 30.0% — ethanol — 50.0% — — — — Cetylpyridinium chloride — — — — — — Glutaraldehyde — — — — — — Water q.s. q.s. q.s. q.s. q.s. q.s. pH 6.29 6.20 6.40 6.34 6.23 2.8

* HOC1 concentration before mixing.

Table 13 Compositions of compositions

Component F98 F99 Fill F113 F114 F136 hypochlorous acid — — — 107ppm* 281ppm* — Polyhexamethylene biguanide 1% 1% 0.12% 1200ppm 1200ppm 10% Isopropyl alcohol 50% 20% 15% — — 45% ethanol — — — — — — Cetylpyridinium chloride — — — — — 5.0% Glutaraldehyde — — — — — — Water q.s. q.s. q.s. q,s q.s. q.s. pH 6.8 6.3 7.60 5.37 3.7 8.29

* HOC1 concentration before mixing.

Table 14 Compositions of compositions

Component F137 F159 F160 F161 F162 F164 hypochlorous acid — — — — — — Polyhexamethylene biguanide 6.0% 5.0% 10% 10% — — Isopropyl alcohol 50.0% 5.0% 5.0% 5.0% 5.0% 50.0% ethanol — — — — — — Cetylpyridinium chloride 4.0% 5.0% — — 10% 10% Glutaraldehyde — — — — — — TRITON® CG-110 — 2.0% 2.0% — — 2.0% Water q.s. q.s. q.s. q.s. q.s. q.s. pH 7.8 7.3 7.0 7.3 6.2 5.6

-64042144

Table 15 Compositions of compositions

Component F165 F166 F167 Hypochlorous acid — — — Polyhexamethylene biguanide — — 10% Isopropyl alcohol 50% — — ethanol — — — Cetylpyridinium chloride 6.0% 10% — Glutaraldehyde — — — TRITON® CG-110 2% 2% 2% Poloxamer-407 — 2% 2% Water q.s. q.s. q.s. pH 6.1 BUT BUT

N/A, not defined.

Example 2 Spore Viability Assays Using Bacillus subtilis.

This example illustrates how to conduct viability analysis using the compositions described in this application.

To prepare pathogenic spore stock cultures, 10 ml of nutrient broth was inoculated using Bacillus subtilis stock culture (ATCC #19659) and incubated on an orbital shaker for 24±2 hours at about 150 rpm at 36±1°C. This culture was used to inoculate Tryptic Soy Agar (TSA) plates. Each dish was inoculated with 500 μl of broth culture and the inoculum was dispensed with a sterile L-shaped dispenser. Furthermore, the purity of said culture was confirmed by streak-plating on TSA plates and incubation of the plates at 36+1°C for 24+2 hours. Each dish was wrapped in parafilm, inverted and incubated for 12-14 days at 36±1°C. After incubation, spores were collected by adding 10 ml of cold sterile water to each dish, the grown culture was removed from the dishes using a distributor, and the resulting suspensions were transferred into sterile 15 ml conical tubes. Suspensions were centrifuged at 5000 rpm (4500xg) for about 10 minutes at room temperature. After removal of the supernatant, the spore pellet was washed by resuspension of the pellet in 10 ml of cold sterile water and centrifuged at 5000 rpm (4500xg) for about 10 minutes. This washing step was repeated two more times. The spore pellet was resuspended in 10 ml of sterile water and the spore suspension was stored at 2-5°C until use. The spore suspension was stained using a staining agent and the spores were examined under a microscope to assess the quality of the spores. At least five fields were examined and the ratio of spores to vegetative cells (or sporangia) was determined. The percentage of spores compared to vegetative cells was approximately 95% spores. Spore titer was determined by preparing serial dilutions, inoculating the dilutions into TSA plates, incubating the plates for 24x2 hours at 36x1°C, and counting the number of colonies formed to determine the titer. The test culture was standardized with phosphate buffer to give a final microbial population of the test culture of 1.0 x 10 ⁶ cfu/ml.

Samples for analysis were prepared with serum, such as, for example, tryptic soy broth (TSB) or bovine serum (BS), or without serum. The goal of the serum was to better mimic the organic load in the blood and other tissues that tends to deactivate the antimicrobial agent. In general, assays were performed by first incubating a sample of pathogen spores with the composition described herein for a period of treatment time, optionally with serum, followed by culturing the sample in a nutrient medium to assess spore viability.

In one series of experiments, assays were performed in which samples were treated with serum to contain 100 µl of a pathogen spore suspension (containing about 1 x 10 ⁶ CFU/ml), 50 µl of serum, and 850 µl of the composition described in Example 1. In assays in which samples not treated with serum, the samples contained 100 µl of the pathogen spore suspension (containing about 1 x 10 ⁶ CFU/ml) and 900 µl of the composition described in example 1. All samples were incubated for 15 minutes and then plated in Petri dishes containing TSA growth medium. The inoculated plates were then incubated at 37°C for 48 hours and the number of bacterial colonies, if any, was counted. It should be noted that when describing certain quantities and volumes, you can use different quantities and volumes. For example, a processed sample may contain 100 µl of a pathogen spore suspension (containing about 1 x 10 ⁶ cfu/ml), 500 µl of serum, and 9400 µl of the composition described in this application. The raw sample may contain 100 µl of the pathogen spore suspension (containing about 1 x ^{10 1} CFU/ml) and 9900 µl of the composition described in this application.

The results of this series of experiments are presented in table. 16. The results indicate that

-65 042144 that compositions F4, F6, F13, F14, F42, F45, F80, F83, F88, F89, and F90 show greater potential as a disinfectant agent for applications involving hard surfaces, devices, and other apparatuses. Compositions showing no growth in treated samples were selected for further analysis as described in Example 3.

Table 16. B. subtilis spore viability assay

Compound Time of processing Incubation time Quantity ^a Serum Free 5% whey 97 ppm NOC1 15 minutes 48 hours 0 >500 0.1% TWEEN® 80 + 64 ppm HOC1 15 minutes 48 hours 43 >500 0.25% glutaraldehyde 15 minutes 48 hours >500 >500 0.5% glutaraldehyde 15 minutes 48 hours >500 >500 fl 15 minutes 48 hours 44 339 F2 15 minutes 48 hours 41 >500 F3 15 minutes 48 hours >500 >500 F4 15 minutes 48 hours 0 0 F6 15 minutes 48 hours 0 0 F13 15 minutes 48 hours 0 0 F14 15 minutes 48 hours 0 0 F15 15 minutes 48 hours >500 >500 F24 15 minutes 48 hours 0 BUT F25 15 minutes 48 hours But 3 F28 15 minutes 48 hours 0 But F42 15 minutes 48 hours But 0 F45 15 minutes 48 hours But 0 F78 15 minutes 48 hours 0 >500 F79 15 minutes 48 hours 0 >500 F80 15 minutes 48 hours 0 0 F81 15 minutes 48 hours 0 >500 F82 15 minutes 48 hours >500 >500 F83 15 minutes 48 hours 0 0 F84 15 minutes 48 hours 0 >500 F85 15 minutes 48 hours 0 >500 F86 15 minutes 48 hours 0 >500 F87 15 minutes 48 hours 0 >500 F88 15 minutes 48 hours 0 0 F89 15 minutes 48 hours 0 0 F90 15 minutes 48 hours 0 0 F91 15 minutes 48 hours 0 >500

^a Values indicated as >500 indicate that there were too many colonies to count (TMTC), so a score over 500 was assigned.

Example 3 Spore Viability Assays Using Bacillus subtilis.

This example illustrates how to conduct viability analysis using the compositions described in this application.

Stock suspensions of pathogen spores were prepared as described in Example 2.

In one series of experiments, samples for analysis were prepared as described in Example 2, except that only treated samples were prepared for each composition, samples were incubated for 30 minutes, a neutralizing agent was used to inhibit the activity of the test microbial agent, for each treatment period after treatment using a neutralizing agent, the samples were serially diluted 1:10 6 times and the treated samples were plated in Petri dishes containing TSA nutrient medium, incubated at a temperature of from 30°C to 35°C for 5 days and counted the number of bacterial colonies, if any. . The purpose of the neutralizing agent, such as, for example, Dee-Engley (DE) broth, was to deactivate the antimicrobial agent, such as hypochlorous acid, polyhexamethylene biguanide, and/or another agent whose activity appears to act as a cleaner, disinfectant or sterilizer,

- 66 042144 thus improving the measurement accuracy at certain time points.

The results are presented in table. 17. The results indicate that compositions F62, F63, F64, and F66 show greater potential as a disinfectant for applications involving hard surfaces, devices, and other apparatuses. Compositions F67, F68, F69, F70, and F71 also show potential as a disinfectant for applications involving hard surfaces, devices, and other apparatuses.

Table 17. B. subtilis spore viability assay

Compound Time of processing Incubation time Quantity ^a 1 2 3 4 5 6 F62 30 minutes 5 days 0 0 0 0 0 52 F63 30 minutes 5 days 0 0 0 0 20 34 F64 30 minutes 5 days 0 0 0 >500 >500 >500 F65 30 minutes 5 days 0 >500 >500 >500 >500 >500 F66 30 minutes 5 days BUT 0 0 0 0 0 F67 30 minutes 5 days 0 0 >500 >500 >500 >500 F68 30 minutes 5 days 0 0 >500 >500 >500 >500 F69 30 minutes 5 days 0 0 >500 >500 >500 >500 F70 30 minutes 5 days 0 0 >500 >500 >500 >500 F71 30 minutes 5 days 0 0 >500 >500 >500 >500 F72 30 minutes 5 days >500 >500 >500 >500 >500 >500 F74 30 minutes 5 days >500 >500 >500 >500 >500 >500 F74 30 minutes 5 days >500 >500 >500 >500 >500 >500 F75 30 minutes 5 days >500 >500 >500 >500 >500 >500 F76 30 minutes 5 days >500 >500 >500 >500 >500 >500

^a Values indicated as >500 indicate that there were too many colonies to count (TMTC), so a score of more than 500 I/O was assigned, not determined.

In other series of experiments, samples for analysis were prepared as described in example 2, except that only processed samples were prepared for each composition, samples were incubated for 30 minutes, 500 μl of a suspension of pathogen spores containing approximately 5x10 ⁶ CFU was used for composition F60 /ml, a neutralizing agent was used to inhibit the activity of the tested microbial agent, for each treatment period after treatment with a neutralizing agent, the samples were serially diluted 1:10 6 times and the treated samples were plated in Petri dishes containing TSA nutrient medium, incubated at a temperature of 35°C ±2°C for 2 days and counted the number of bacterial colonies, if any.

The results are shown in Table 18. Compositions F28,F136hF137 showed a decrease in spore viability of B. subtilis by more than 6 orders of magnitude, and composition F60 showed a decrease in spore viability by 6 orders of magnitude. The results indicate that compositions F28, F60, F136hF137 are effective disinfectants.

Table 18. Viability analysis of B. subtilis spores

Compound Time of processing Incubation time Quantity ^a 1 2 3 4 5 6 F28 30 minutes 48 hours 0 0 0 0 0 0 F60 30 minutes 48 hours 0 0 0 0 0 0 F136 30 minutes 48 hours 0 0 0 0 0 0 F137 30 minutes 48 hours 0 0 0 0 0 0

^a Values indicated as >500 indicate that there were too many colonies to count (TMTC), so a score over 500 was assigned.

Example 4 Stability Studies

This example illustrates how to conduct stability studies of compositions using the compositions described in this application.

Stock suspensions of pathogen spores were prepared as described in Example 2.

In one series of experiments, the compositions described in this application were prepared, loaded into a bottle and stored at room temperature for 6 months. Samples of compositions stored in bottles for analysis were prepared as described in example 2, except that only processed samples were prepared for each composition, samples were incubated for 30 minutes, the suspension of pathogen spores contained approximately 1x10 $ cfu / ml, for inhibition activity of the tested microbial agent, a neutralizing agent was used, for each treatment period after treatment with a neutralizing agent, the samples were serially diluted 6 times 1:10 and the treated samples

-67042144 were seeded in Petri dishes containing TSA nutrient medium, incubated at a temperature of from 30°C to 35°C for 5 days, and on the second day of incubation, the number of bacterial colonies was counted, if any.

The results are presented in table. 19. Composition F60 demonstrated a decrease in the viability of B. subtilis spores by 6 orders of magnitude. The results indicate that the composition F60 is an effective disinfectant, and its effectiveness is maintained for at least 6 months.

Table 19. B. subtilis spore viability assay

Compound Time of processing Incubation time Quantity ^a 1 2 3 4 5 6 F60 30 minutes 48 hours 0 0 0 0 0 0

^a Values indicated as >500 indicate that there were too many colonies to count (TMTC), so a score over 500 was assigned.

Example 5 Bacterial viability assays using Clostridium difficile.

This example illustrates how to conduct stability studies of compositions using the compositions described in this application.

Clostridium difficile pathogen stock suspensions were prepared by a third party contract laboratory according to standard industry procedures.

In one series of experiments, sample compositions for analysis were prepared as described in Example 2, except that only treated samples were prepared for each composition, samples were incubated for 1 minute, a neutralizing agent was used to inhibit the activity of the test microbial agent, for each treatment period after treatment with a neutralizing agent, the samples were serially diluted 1:10 6 times and the treated samples were plated in Petri dishes containing TSA nutrient medium, incubated at a temperature of from 30°C to 35°C for 2 days and counted the number of bacterial colonies, when they availability.

The results are presented in table. 20. Composition F60 demonstrated a decrease in the viability of C. difficile spores by 6 orders of magnitude. The results indicate that composition F60 is an effective disinfectant against C. difficile bacteria as well as B. subtilis spores.

Table 20 C. difficile viability assay

Compound Time of processing Incubation time Quantity ^a 1 2 3 4 5 6 F60 1 minute 48 hours 0 0 0 0 0 0

^a Values indicated as >500 indicate that there were too many colonies to count (TMTC), so a score over 500 was assigned.

Example 6 Disinfection of a medical device.

Stock suspensions of pathogen spores were prepared as described in Example 2.

Test analysis was performed by placing the endoscope in a sterile container inside a biological safety cabinet and inoculating said device with 4.0 ml of test inoculum solution containing 1x106 CFU/ml of pathogenic spore suspension and 5% organic soil was used to mimic the level of contamination of the endoscope in clinical use. . The contaminated endoscope was then removed from the test inoculum solution and air dried for one hour. The contaminated device was then transferred to a sterile plastic container inside the biosafety cabinet and cleaned with F60 (see example 1). After the cleaning process, the cleaned device was transferred to a sterile plastic container inside the biosafety cabinet and extracted with sterile water. Water after extraction was filtered through a 0.45 μm membrane filter. The filter was then used to inoculate 9 ml of culture broth. Two 10-fold dilutions were then prepared from the inoculated broth, except that the last dilution was prepared in TSM-B broth. Each extracted solution was incubated for 14 days at 30-35°C. Visual turbidity was assessed and compared with negative and positive controls. In the positive controls, the contaminated device was not cleaned with the test compound or any other disinfectant, but subjected to an extraction process. In negative controls 1) the device was incubated in a control inoculum solution that was identical to the test inoculum solution, except that the solution was free of pathogenic spore suspension and organic soil; and 2) the device was left unfolded and subjected to the same cleaning and extraction process as the device under test.

Composition F60 demonstrated a decrease in the viability of b. subtilis spores by 6 orders of magnitude. The results indicate that the F60 composition effectively disinfected the inoculated device with an exposure time of no more than 30 minutes.

In conclusion, it should be understood that while aspects of the present invention are illustrated with reference to specific embodiments, one skilled in the art will readily appreciate that

- 68 042144 these described implementation options are only an illustration of the principles of the subject matter of the invention described in this application. Therefore, it should be understood that the described subject matter is not limited in any way to the specific compound, composition, article of manufacture, apparatus, methodology, protocol and/or reagent, and the like described in this application, unless expressly stated otherwise. In addition, those skilled in the art will recognize that certain changes, modifications, permutations, corrections, additions, deletions, and subcombinations thereof may be made in accordance with the teachings presented herein without departing from the spirit of the present invention. Thus, the following claims of the appended claims and claims that may be added after filing the application are intended to be interpreted as including all such changes, modifications, permutations, corrections, additions, deletions and sub-combinations, as fall within their actual spirit and scope. .

This application describes some embodiments according to the invention, including the best way known to the authors of the present invention, the implementation of the present invention. Of course, changes to these described embodiments will be apparent to those skilled in the art upon reading the above description. The inventors of the present invention expect those skilled in the art to apply such modifications as necessary, and the inventors of the present invention believe that the present invention may be practiced in a manner other than those specifically described in this application. Accordingly, the present invention includes all modifications and equivalents of the subject matter set forth in the appended claims, within the limits of applicable law. In addition, the present invention covers any combination of the embodiments described above in all their possible variants, unless otherwise indicated in this application, or the context clearly indicates otherwise.

Groupings of alternative implementations, elements or steps according to the invention should not be construed as limitations. Each member of the group may be mentioned and claimed individually or in any combination with other members of the group described in this application. It is contemplated that one or more members of the group may be included in or excluded from the group for reasons of convenience and/or patentability. When making such an inclusion or deletion, the description is believed to include the modified group, thus fully complying with the description of all Markush groups used in the claims of the appended claims.

Unless otherwise indicated, all numbers expressing a characteristic, subject, quantity, parameter, property, term, etc. used in this application and claims, should be understood as modified in all cases by the term approximately. As used herein, the term roughly means that a feature, item, quantity, parameter, property, or term thus qualified spans a range of plus/minus ten percent above or below the value of said feature, item, quantity, parameter, property, or term. Accordingly, unless otherwise indicated, the numerical parameters presented in the description and claims of the appended claims are approximate values that may vary. For example, because mass spectrometry instruments can vary slightly in determining the mass of a given analyte, the term roughly in the context of ion mass or ion mass/charge ratio refers to +/-0.50 atomic mass unit. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical indication should be read at least in terms of the number of significant figures given and using conventional rounding techniques.

The use of the terms can or is able in relation to an implementation option or aspect of an implementation option also includes the alternative meaning cannot or is not capable. As such, if this application describes that an implementation option or aspect of an implementation option may or may be included as part of the subject matter of the present invention, then a negative limitation or exclusionary condition is also explicitly intended to mean that the implementation option or aspect of the implementation option may not be included or not capable of being included as part of the subject matter of the present invention. Similarly, the use of the term optionally in relation to an implementation or aspect of an implementation means that such an implementation or aspect of an implementation option may be included as part of the subject of the present invention or may not be included as part of the subject of the present invention. The applicability of such a negative limitation or exclusionary condition depends on whether the negative limitation or exclusionary condition is specified in the claimed subject matter.

Although the numerical ranges and values representing the broad scope of the present invention are approximate, the numerical ranges and values given in the specific examples are given as precisely as possible. However, any numerical range or value, by definition, includes certain errors which are the necessary result of the standard deviation found in their respective test measurements. The listing in this application of numerical ranges of values is intended only as a shorthand way of presenting individual references to each individual numerical value that is in the

- 69 042144 affairs range. Unless otherwise specified in this application, each individual value of the numerical range is included in the present description, as if it were separately specified in this application.

Singular forms and similar references used in the context of the description of the invention (especially in the context of the following claims) cover the singular and plural forms, unless otherwise indicated in this application, or the context clearly indicates otherwise. In addition, ordinal designators, such as first, second, third, etc., for identified elements are used to distinguish elements, and they do not indicate or imply a required or limited number of such elements, and do not indicate the specific position or order of such elements, unless specifically stated otherwise. All methods described in this application can be carried out in any suitable order, unless otherwise indicated in this application, or the context clearly indicates otherwise. The use of any and all examples or exemplary language (eg, such as) presented in this application is only intended to better demonstrate the present invention and does not limit the scope of the present invention as otherwise claimed. No expression in the present description should be taken as an indication of any unclaimed element essential to the practice of the present invention.

When used in claims, whether used at the time of filing or added in revisions, the open transition term containing (and its equivalent open transition phrases, such as including, containing, and having) covers all explicitly stated elements, limitations, steps and/or distinctive features, alone or in combination with an unspecified subject matter; however, the named elements, limitations and/or features are essential, and other unnamed elements, limitations and/or features can be added while maintaining the formation of the structure within the scope of the claims. Specific implementations described in this application may be further limited in the claims using closed transitional phrases consisting of or consisting essentially of instead of or as an addition to the term containing. When used in claims, whether in use at the time of filing or added in revisions, the closed transition phrase consisting of excludes any element, limitation, step, or feature not expressly stated in the claims. A closed transitional phrase consisting essentially of limits the scope of the claims to the expressly stated elements, limitations, steps and/or features and any other elements, limitations, steps and/or features that do not materially affect the essential and novel features of the claimed subject matter. . Thus, the meaning of the open passphrase containing is defined to encompass all of the explicitly stated elements, limitations, steps, and/or features, as well as any optional, additional, unspecified elements, limitations, steps, and/or features. Thus, the meaning of the closed passphrase consisting of is defined to include only the elements, limitations, steps, and/or features expressly stated in the claims, while the meaning of the close passphrase consisting essentially of is defined to include only the elements, limitations, steps and/or features explicitly stated in the claims, and elements, limitations, steps and/or features that do not significantly affect the main and new characteristics of the claimed subject matter. Thus, the open passphrase containing (and its equivalent open passphrases) includes in its meaning, as a limiting case, the claimed subject matter, defined by closed passphrases consisting of or consisting essentially of. Therefore, such embodiments described herein or claimed by the phrase consisting of, are explicitly or by definition unambiguously described, provided, and supported in this application by the phrases consisting essentially of and consisting of.

All patents, patent publications and other publications referred to and identified in this specification are individually and expressly incorporated herein in their entirety by reference for the purpose of describing and disclosing, for example, the compositions and methodologies described in such publications, which can be used in the context of the present invention. These publications are provided solely for their disclosure prior to the filing date of this application. Nothing in this regard should be construed as an admission that the authors of the present invention are not entitled to precede such disclosure by virtue of prior invention or for any other reason. All statements as to the date or representation as to the contents of said documents are based on information available to the applicants and do not constitute any admission as to the correctness of the dates or contents of the said documents.

Finally, the terminology used in this application is only intended to describe specific embodiments and is not intended to limit the scope of the present invention, which is defined only by the claims. Accordingly, the present invention is not limited to the description presented.

Claims (49)

CLAIM 1. A disinfectant composition containing at least 3% by weight of the composition to not more than 15% by weight of the composition of one or more compounds containing a biguanide fragment, and from 40 to 70% by weight of the composition of one or more alcohols representing C ₁ -C ₅ linear monohydric alcohols, wherein said one or more C1-C5 linear monohydric alcohols does not include ethanol. 2. Disinfectant composition according to claim 1, characterized in that one or more compounds containing a biguanide fragment include formula II NH NH where each R ⁵ is independently a bond, H, C1-10 alkyl, or a 5- or 6-membered aromatic ring optionally substituted with halogen; each R ⁶ is independently C1-10 alkanediyl, C _1-10 alkyndiyl; and n is 1-5. 3. Disinfectant composition according to claim 2, characterized in that one or more compounds containing a biguanide fragment include polyhexamethylene biguanide (PHMB), polyaminopropyl biguanide (PAB), 1,1'-(1,6-hexanediyl)bis {2-[ N'-(2-ethylhexyl)carbamimidoyl]guanidine} (alexidine), chlorhexidine, chlorhexidine gluconate, or any combination thereof. 4. Disinfectant composition according to any one of claims 1 to 3, characterized in that one or more C1-C5 linear monohydric alcohols are methanol, propanol, isopropanol, butanol or pentanol. 5. Disinfectant composition according to claim 4, characterized in that one or more C1-C5 linear monohydric alcohols are propanol, isopropanol, butanol or pentanol. 6. A disinfectant composition according to claim 4 or 5, characterized in that one or more C1-C5 linear monohydric alcohols is isopropanol. 7. Disinfectant composition according to any one of claims 1 to 6, characterized in that one or more compounds containing a biguanide fragment are contained in an amount of from 6.0 to 14.0% by weight of the composition. 8. Disinfectant composition according to claim 7, characterized in that one or more compounds containing a biguanide fragment are contained in an amount of from 8.0 to 12.0% by weight of the composition. 9. A disinfectant composition according to any one of claims 1 to 8, characterized in that one or more C1-C5 linear monohydric alcohols are contained in an amount of at least 45% by weight of the composition. 10. A disinfectant composition according to claim 9, characterized in that one or more C1-C5 linear monohydric alcohols are contained in an amount of at least 50% by weight of the composition. 11. A disinfectant composition according to any one of claims 1-9, containing from 8 to 12% by weight of the composition of one or more compounds containing a biguanide fragment, and from 45 to 70% by weight of the composition of one or more C1-C5 linear monohydric alcohols. 12. A disinfectant composition according to any one of claims 1-7 or 9, containing from 4 to 8% by weight of the composition of one or more compounds containing a biguanide fragment, and from 45 to 55% by weight of the composition of one or more C1-C5 linear monoatomic alcohols. 13. A disinfectant composition according to any one of claims 1 to 12, further comprising one or more quaternary ammonium surfactants. 14. A disinfectant composition according to claim 13, wherein the one or more quaternary ammonium surfactants comprise quaternary ammonium surfactants containing C ₁₂ . ₁₈ alkyl chains, quaternary ammonium surfactants containing aromatic ring groups, quaternary ammonium surfactants containing dual quaternary ammonium surfactants containing C ₁₂ . ₁₈ alkyl chains, and alkyl dimethyl ammonium chloride mixtures, quaternary ammonium surfactants containing double chain dialkylmethylamines, quaternary ammonium surfactants containing synergistic combinations of double quaternary ammonium compounds, quaternary ammonium surfactants containing polymeric quaternary ammonium compounds, or quaternary ammonium surfactants containing bis-quaternary ammonium compounds with polymeric quaternary ammonium compounds. 15. A disinfectant composition according to claim 13, wherein the one or more quaternary ammonium surfactants comprise benzalkonium chloride (BAC), benzethonium chloride (BZT), benzododecinium bromide (or dimethyldodecylbenzylammonium bromide), bronidox (or 5bromo-5- nitro-1,3-dioxane), carbetopendecinium bromide, cetalkonium chloride (CKC), cetrimonium, cetrimide, cetylpyridinium chloride (CPC), cetyltrimethylammonium bromide (CTAB or cetrimonium bromide), and - 71 042144 cetyltrimethylammonium chloride (STAC or cetrimonium chloride), didecyldimethylammonium chloride (DDAC), dioctadecyldimethylammonium bromide (DODAB or dimethyldioctadecylammonium bromide), dioctadecyldimethylammonium chloride (DODAC or dimethyldioctadecylammonium chloride), docosyltrimethylammonium chloride, docosyltrimethylammonium chloride (DCTAC) , methylbenzetonium chloride, octenidine dihydrochloride, polydronium chloride, stearylalkonium chloride (or dimethylbenzyl octadecylammonium), tetraethylammonium bromide, tetramethylammonium hydroxide (TMAH), or tonzonium bromide. 16. A disinfectant composition according to any one of claims 13 to 15, characterized in that one or more quaternary ammonium surfactants are contained in an amount of from 2.0 to 10.0% by weight of the composition. 17. A disinfectant composition according to claim 16, characterized in that one or more quaternary ammonium surfactants are contained in an amount of from 2.0 to 7.0% by weight of the composition. 18. Disinfectant composition according to any one of claims 1-9 or 13-17, containing from 8 to 12% by weight of the composition of one or more compounds containing a biguanide fragment, from 45 to 70% by weight of the composition of one or more C ₁ -C ₅ linear monohydric alcohols and 2 to 7% cetylpyridinium chloride. 19. Disinfectant composition according to any one of claims 1-8 or 13-17, containing from 8 to 12% by weight of the composition of one or more compounds containing a biguanide fragment, from 40 to 50% by weight of the composition of one or more C1-C ₅ linear monohydric alcohols and from 2 to 7% cetylpyridinium chloride. 20. Disinfectant composition according to claim 18 or 19, characterized in that cetylpyridinium chloride is contained in an amount of 5% by weight of the composition. 21. A disinfectant composition according to any one of claims 1 to 20, further comprising one or more carriers. 22. The disinfectant composition of claim 21, wherein the one or more carriers comprise an aqueous carrier, a semi-solid carrier, a solid carrier, or any combination thereof. 23. A disinfectant composition according to any one of claims 1 to 22, further comprising one or more additional ingredients. 24. The disinfectant composition of claim 23, wherein the one or more additional ingredients include a preservative, a chelating agent, or any combination thereof. 25. A disinfectant composition according to any one of claims 1 to 24, having a pH of 6 to 8, 6.7 to 7.7, 6.8 to 7.6, 6.9 to 7.5, 7, 0 to 7.4, 7.1 to 7.3 or 7.2. 26. Disinfectant composition according to any one of claims 1 to 24, having a pH of 5.0 to 6.5, 5.2 to 6.2, 5.3 to 6.1, 5.4 to 6.0 , 5.5 to 5.9, 5.6 to 5.8, or 5.7. 27. Disinfectant composition according to any one of claims 1 to 24, having a pH of 5.3 to 6.8, 5.5 to 6.5, 5.6 to 6.4, 5.7 to 6.3 , 5.8 to 6.2, 5.9 to 6.1, or 6.0. 28. A disinfectant composition consisting of at least 3.0% by weight of the composition to not more than 12% by weight of the composition of one or more compounds containing a biguanide fragment, from 40 to 70% by weight of the composition of one or more alcohols representing C1 -C ₅ linear monohydric alcohols, and water, where one or more compounds containing a biguanide fragment is polyhexamethylene biguanide (PHMB), polyaminopropyl biguanide (PAB), 1,1'-(1,6hexanediyl)bis{2-[K'- (2-ethylhexyl)carbamimidoyl]guanidine} (alexidine) or any combination thereof; and where one or more C1-C ₅ linear monohydric alcohols does not include ethanol. 29. The disinfectant composition of claim 28, further comprising 2 to 10% of one or more quaternary ammonium surfactants, wherein the one or more quaternary ammonium surfactants comprise cetylpyridinium chloride. 30. A disinfectant composition consisting of 5 to 14% by weight of the composition of polyhexamethylene biguanide, from 60 to 80% by weight of the composition of one or more alcohols, which are C1-C ₅ linear monohydric alcohols, and water, where one or more C1-C ₅ linear monohydric alcohols does not include ethanol. 31. Disinfectant composition according to claim 30, characterized in that polyhexamethylene biguanide is contained in an amount of 8 to 12% by weight of the composition. 32. A disinfection kit containing a disinfectant composition according to any one of claims 1 to 31, one or more delivery or application systems, instructions, or container. 33. A disinfection kit according to claim 32, additionally containing a washing solution. 34. A set for disinfection, designed to obtain a disinfectant composition according to any one of claims 1 to 32, containing a first component containing one or more compounds containing a biguanide fragment, a second component containing one or more C1-C ₅ linear monohydric alcohols, one or more delivery or application systems. 35. A disinfection kit according to claim 34, further comprising a third component containing one or more quaternary ammonium surfactants. - 72 042144 36. A disinfection kit for obtaining a disinfectant composition according to any one of claims 1 to 32, containing a first component containing isopropyl alcohol and a second component containing polyhexamethylene biguanide. 37. A disinfection kit according to any of pi.34-36, additionally containing a third component containing a solution for washing, and / or one or more delivery or application systems. 38. A disinfection kit according to any one of claims 34, 35 or 37, characterized in that one or more compounds containing a biguanide fragment include formula II where each R ⁵ is independently a bond, H, Cio alkyl, or a 5- or 6-membered aromatic ring optionally substituted with halogen; each R ⁶ is independently Cio alkanediyl, Cio alkyndiyl; and η is 1-5. 39. The disinfection kit according to i.38, characterized in that one or more compounds containing a biguanide fragment include polyhexamethylene biguanide (PHMB), polyaminopropyl biguanide (PAB), 1,1 '-(1,6-hexanediyl) bis {2- [H'-(2-ethylhexyl)carbamimidoyl]guanidine} (alexidine), chlorhexidine, chlorhexidine gluconate, or any combination thereof. 40. Disinfection kit according to any one of claims 34, 35 or 37-39, characterized in that one or more C1-C5 linear monohydric alcohols are methanol, ethanol, propanol, isopropanol, butanol, pentanol and 1-hexadecanol. 41. A method for cleaning, disinfecting and/or sterilizing a device, comprising applying a disinfectant composition according to any one of claims 1 to 31 or applying components according to any one of claims 3240 to the device, wherein applying the composition cleans, disinfects and/or sterilizes the device. 42. A method for cleaning, disinfecting and/or sterilizing a surface area, comprising applying a disinfectant composition according to any one of pi. 1-31 or applying the components of any one of claims 32-40 to a surface area, wherein applying the composition cleans, disinfects and/or sterilizes the surface area. 43. The method of claim 42, wherein the surface area is a porous surface area or a non-porous surface area. 44. The method according to claim 42 or 43, characterized in that the surface area includes the top surface of the table, countertop, floor, wall, ceiling, window, door, doorknob, shower, bathtub, sink, faucet, toilet bowl, toilet seat, drain , equipment, machinery, personal protective equipment, personal biological protection equipment, medical device, dental device, pharmaceutical device, veterinary device, mortuary device or human skin. 45. The method according to claim 44, characterized in that the medical device is a surgical instrument, an instrument for respiratory therapy, an instrument for anesthesia, a catheter, an implant, a probe, an endoscope, an arthroscope, a laparoscope, a blade, a cystoscope, a spirometer, a mask and tubes for CPAP therapy, dialysis instrument and accessories, ECMO heart-lung machine and accessories, heart-lung machine and accessories, and diaphragm adjustment ring. 46. A method of cleaning, disinfecting and/or sterilizing a microbial infection in a patient, including the use of a disinfectant composition according to any one of paragraphs. 1-31 or application of the components according to any one of claims 32-40 on a patient, wherein the application of the composition cleans, disinfects and/or sterilizes the patient. 47. The method of claim 46, wherein the composition is applied topically or administered enterally or parenterally. 48. The method according to any one of claims 42-47, characterized in that the composition is applied daily, every other day, two days, once a week, several times a week, once a month, several times a month, once a year or several once a year, if desired. 49. The method according to any one of claims 42-48, characterized in that the composition is applied several times a day.