EA040154B1 - BACTERICIDAL AND FUNGICIDAL COMPOSITION - Google Patents

Description

Technical field

The present invention relates to the field of crop protection, in particular to a fungicidal composition with improved properties, and in particular to a fungicidal composition comprising benzisothiazolinone and mancozeb.

State of the art

Benzisothiazolinone compounds are new broad spectrum fungicides that are mainly used to control the spread and treatment of various bacterial and fungal diseases of cereals, vegetables and fruits. The mechanisms of fungicidal action mainly include the destruction of the structure of the nuclei of harmful fungi, causing their death due to the loss of the nucleus component and interfering with the metabolism of fungal cells, causing physiological distress, ultimately leading to death. When the agent is applied at an early stage of disease development, plants can be effectively protected from infection by pathogens; and when the agent is applied in a suitable increased amount after the development of the disease, the spread of harmful fungi is well controlled, thus achieving a dual effect of protection and killing.

Mancozeb is a protective fungicide with high efficacy, low toxicity, high digestibility and broad fungicidal spectrum, which is mainly used to control the spread of various fungal diseases on wheat, fruits and vegetables. The mechanisms of fungicidal action are such that after application, a layer of breathable, water-permeable and light-transmitting dense protective film is formed on the surface of leaves or fruits, inhibiting the germination and penetration of fungal spores and destroying the activity of various enzymes necessary for the normal physiological metabolism of fungi, thus achieving the goal of sterilization and disease prevention.

Practical experience in the field of pesticides has shown that repeated and targeted application of the active compound to control the spread of harmful fungi in many cases leads to a rapid selection of fungal strains. To reduce the risk of selecting resistant fungal strains to control the spread of harmful fungi, a mixture of compounds with different activities is currently commonly used. By combining active compounds with different mechanisms of action it is possible to delay the development of resistance, reduce application rates and reduce costs.

Brief description of the invention

In view of the problems of developing resistance and detecting soil residues of fungicides in practical applications, the purpose of this invention is to screen fungicides with different mechanisms of bactericidal action, which are combined to obtain a new fungicidal composition so as to enhance the anti-development effect of bactericides, delay the development of resistance, reduce the amount applied and reduce price.

Another object of the present invention is to provide a method for producing a fungicidal composition containing components A and B, and its use for preventing the development of pests on crops in an agricultural area.

The objectives of the present invention can be achieved by the following means.

The fungicidal composition having a synergistic effect contains active components A and B, where the active component A is a compound having the structure of formula (I) and the active component B is mancozeb.

In formula (I) R is selected from H or C ₁ -C ₈ alkyl.

In the present invention, C ₁ -C ₈ alkyl refers to a straight or branched alkyl group having 1 to 8 carbon atoms and includes C4 alkyl (i.e. methyl), C2 alkyl (i.e. ethyl), C ₃ alkyl (i.e. n-propyl and iso-propyl), C4 alkyl (i.e. n-butyl, iso-butyl, t-butyl and sec-butyl), C5 alkyl (i.e. npentyl and the like), C6 alkyl, C ₇ alkyl and C ₈ alkyl, including but not limited to C ₁ -C ₆ alkyl, C 1 -C 8 alkyl and C ₁ -C ₄ alkyl.

In a preferred implementation, R is selected from H or C ₁ -C ₄ -alkyl.

In another preferred implementation, R is selected from H, -CH ₃ or -C4H9.

In formula (I), when R is H, A is 1,2-benzisothiazolin-3-one (BIT).

In formula (I), when R is CH ₃ , A is 2-methyl-1,2-benzisothiazolin-3-one (MBIT).

In formula (I), when R is C4H9, A is 2-n-butyl-1,2-benzisothiazolin-3-one (BBIT) where butyl is n-butyl in the formula.

Through experimentation, the inventor has found that the composition of the present invention has a remarkable synergistic effect in controlling the spread of bacterial or fungal diseases in crops and, more importantly, the application rate is reduced and the cost is reduced. The compounds that make up compounds A and B have different structures and different mechanisms of action, and the combination of the two compounds to a certain extent can expand the spectrum of fungicidal action and slow down the emergence and development of fungal resistance. Also, there is no cross-resistance between compounds A and B.

The weight ratio between the two components in the fungicidal composition of the present invention is 1:3-300. In a preferred embodiment, the weight ratio between active ingredient A and active ingredient B is 1:4-280. In order to make the synergistic effect between the two components more prominent, the weight ratio between active component A and active component B may further preferably be 1:10-270.

In a preferred embodiment, the weight ratio between active ingredient A and active ingredient B can be desirably set to any of 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1: 10, 1:20, 1:30, 1:40, 1:50, 1:60, 1:70, 1:80, 1:90, 1:100, 1:110, 1:120, 1:130, 1:140, 1:150, 1:160, 1:170, 1:180, 1:190, 1:200, 1:210, 1:220, 1:230, 1:250, 1:260, 1: 270, 1:280, 1:290 and 1:300, or may be selected within the range defined by any two of the above ratios.

The composition of the present invention may be formulated as a pesticidally acceptable composition further comprising active ingredients and a pesticide excipient or adjuvant.

In one implementation, the content of active components in the composition is 2-95%, and preferably is 5-95% by weight.

In another implementation, the composition is in the composition of a pesticidally acceptable composition containing 10-90% (wt.) active ingredients and 90-10% (wt.) pesticidal adjuvant.

The present invention provides the use of a bactericidal composition comprising components A and B in the control of the spread of crop diseases in the agricultural field, in particular in the control of the spread of fungal or bacterial diseases of certain crops.

The composition may specifically include a pesticide adjuvant such as one or more carriers, solvents, dispersants, wetting agents, binders, thickeners, adhesives, surfactants, fertilizers, and the like. The commonly used adjuvant may be mixed during application.

A suitable adjuvant or adjuvant may be a solid or liquid, which are typically materials often used in formulating a composition, such as a natural or regenerated agent, a solvent, a dispersing agent, a wetting agent, an adhesive, a thickener, or a binder.

The composition of the present invention can be applied by applying the composition of the present invention to the aerial parts of plants, in particular leaves or foliage surfaces. The composition may be used to impregnate seeds or may be applied to the surface of objects whose spread is to be controlled. The frequency of application and dosage depend on the biology of the pathogen and the climatic and housing conditions. The place where the plant grows, such as a rice field, can be treated with a liquid composition of the composition, or the composition is applied to the soil in solid form, for example in the form of granules (soil application), when the composition penetrates the plant through the roots through the soil (systemic action).

The composition of the present invention may be formulated into a variety of pesticidally acceptable formulations, including, but not limited to, emulsifiable concentrates, suspensions, wettable powders, water-dispersible granules, powders, granules, aqueous solutions, aqueous emulsions, microemulsions, baits, mother liquor, mother powder, and the like. Further. In a preferred embodiment, the composition of the present invention is a wettable powder, suspension, water-dispersible granules, aqueous emulsion or microemulsion. Depending on the properties of the compositions, the purposes to be achieved by the application of the compositions, and the environmental conditions, the compositions may be applied by spraying, pulverizing, dusting, spreading or pouring.

The composition of the present invention can be prepared in various compositions by known methods. The active ingredients can be mixed uniformly with an adjuvant such as a solvent or solid carrier and, if necessary, with a surfactant and milled to obtain the desired composition.

The solvent may be selected from aromatic hydrocarbons containing preferably from 8 to 12 carbon atoms, for example mixtures of xylenes, substituted benzene or phthalate esters, for example dibutyl or dioctyl phthalate; aliphatic hydrocarbons, for example cyclohexane or paraffin; alcohols, glycols and their ethers and esters, for example ethanol, ethylene glycol and ethylene glycol monomethyl ether; ketones, for example cyclohexanone; highly polar solvents, such as K-methyl-2-pyrrolidone, dimethyl sulfoxide or dimethylformamide; and vegetable oils, such as soybean oil.

The solid carrier includes, for example, natural mineral fillers commonly used in powders and dispersible powders, such as talc, kaolin, montmorillonite, or activated bauxite. Highly dispersed silicic acid or a highly dispersed absorbent polymer carrier, such as a granular absorbent carrier or a non-absorbent carrier, may also be added to control the physical properties of the composition. A suitable granular absorbent carrier is porous, such as pumice, fat clay or bentonite. A suitable non-absorbent carrier includes, for example, calcite or sand. Moreover, a large number of inorganic and organic materials pre-prepared in the form of granules, and especially dolomite, can be used as a carrier.

As desired based on the chemical nature of the active ingredients, in the composition of the present invention, a suitable surfactant includes lignosulfonic acid, naphthalene sulfonic acid, phenolsulfonic acid, alkaline earth metal or amine salts, alkylaryl sulfonates, alkyl sulfates, alkyl sulfonates, fatty alcohol sulfates, fatty acids, and sulfated ethers. ethylene glycol and fatty alcohol, condensation products of sulfonated naphthalene and naphthalene derivatives with formaldehyde, condensation products of naphthalene or naphthalenesulfonic acid with phenol and formaldehyde, polyoxyethylene octylphenyl ethers, ethoxylated iso-octylphenol, octylphenol, nonylphenol, polyoxyethylene alkylaryl ethers, polyethylene glycol tributylphenyl ether, polyethylene glycol tristearylphenyl ether, alkyl aryl alcohol polyesters, ethoxylated castor oil, polyoxyethylene alkyl ethers, ethyl condensates oxide, ethoxylated polyoxypropylene, polyethylene glycol and lauryl acetal ether, sorbates, lignin sulfite waste liquid and methyl cellulose.

When a liquid formulation is prepared, active component A may first be dissolved in the base material to form the benzisothiazoline metal salt. Suitable base materials include alkali metal carbonates, alkali metal hydroxides (eg sodium or potassium hydroxide), alkali metal alkoxycarbonates, alkali metal alkoxides or magnesium methoxide.

The two active ingredients in the composition of the present invention have a synergistic effect such that the activity of the composition is markedly higher than the corresponding activity or the expected sum of the respective activities of each individual compound. The synergistic effect is manifested in a reduced application rate, an expanded spectrum of fungicidal activity, a rapid onset of action, a long-term effect of preventing the development of the disease, an improved prevention of the development of harmful fungi on plants with a single or few applications, an increased possible interval between applications. These characteristics are especially required in the practice of preventing the development of fungi on plants.

The fungicidal and bactericidal composition of the present invention is useful for preventing crop diseases in the agricultural field, and particular diseases that can be treated include, but are not limited to, bacterial peach leaf spot, tobacco grouse, rice stem blight, cucumber corner leaf spot, cucumber downy mildew. , rice bacterial streak, rice bacterial wilt, corn bacterial wilt, watermelon fusarium wilt, grape downy mildew, tomato bacterial wilt, eggplant bacterial wilt, rice downy sooty fungus, rice bacterial streak, pepper anthracnose, lychee ulcer, grape anthracnose, tobacco bacterial wilt, cucumber anthracnose, celery leaf spot, lotus root rot, strawberry powdery mildew, lettuce downy mildew, celery gray rot, apricot bacterial leaf spot, peach ulcers, downy mildew powdery mildew of onion, bacterial corner leaf spot of cotton, bacterial abscission of cucumber leaves, and the like.

The composition of the present invention exhibits the following additional qualities.

1) The composition of the present invention produces a noticeable synergistic effect.

2) Since the two individual agents in the composition of the present invention have very different structures and completely different mechanisms of action, no cross-resistance is observed, so that the problem of development of resistance arising from the use of each of the agents alone is delayed.

3) The composition of the present invention is safe for crops and has good efficiency.

The study shows that the fungicidal composition of the present invention has stable chemical properties, significant synergistic effects and noticeable synergistic and additional effects in relation to the objects, the distribution of which should be controlled.

Detailed description of the invention

To make the objectives, technical solution and advantages of the present invention more clear, the present invention is described in more detail with reference to examples. It should be understood that the specific examples described below are for illustration purposes only and do not limit the present invention. It may be made any modifications and equivalent improvements and replacements, which are all within the scope of protection of the present invention, without departing from the idea and principle of the present invention.

The percentages given in all formulations in the following examples are all weight percent. Various formulations, which may vary as desired, are prepared from the composition of the present invention in a manner known in the art.

I. Examples of preparation of compositions.

(I) Preparation and examples of water-dispersible granules.

The active ingredients A and B, the adjuvant and the excipient are uniformly mixed according to the composition ratio, finely pulverized by a gas flow into a wettable powder, then an amount of water is added, mixed, extruded, granulated, dried and sieved to obtain a water-dispersible granular product.

1) Preparation of water-dispersible granules of active ingredient A (BIT) and active ingredient B.

Example 1: Water-dispersible granules 71% BIT-mancozeb.

BIT 1%, mancozeb 70%, potassium dodecylsulfonate 5%, ammonium sulfate 3%, potassium alkylnaphthalenesulfonate 4%, finely ground calcium carbonate up to 100%.

Example 2: Water-dispersible granules 50.5% BIT-mancozeb.

BIT 0.5%, mancozeb 50%, sodium methylnaphthalenesulfonate condensate with formaldehyde 5%, sodium dodecyl sulfate 3%, sodium lignin sulfonate 6% and diatomaceous earth up to 100%.

Example 3: 25.1% BIT-mancozeb water-dispersible granules.

BIT 0.1%, mancozeb 25%, sodium carboxymethyl starch 2%, sodium dodecyl sulfate 4%, xanthan gum 2%, sodium lignin sulfonate 6% and attapulgite up to 100%.

2) Preparation of water-dispersible granules of active ingredient A (MBIT) and active ingredient B.

Example 4: 71% MBIT-mancozeb water-dispersible granules.

MBIT 1%, mancozeb 70%, other components are the same as in example 1.

Example 5: 50.5% MBIT-mancozeb water-dispersible granules.

MBIT 0.5%, mancozeb 50%, other components are the same as in example 2.

Example 6 25.1% MBIT-mancozeb water dispersible granules.

MBIT 0.1%, mancozeb 25%, other components are the same as in example 3.

3) Preparation of water-dispersible granules of active ingredient A (BBIT) and active ingredient B.

Example 7: 71% BBIT-mancozeb water-dispersible granules.

BBIT 1%, mancozeb 70%, other components are the same as in example 1.

Example 8: 50.5% BBIT-mancozeb water-dispersible granules.

BBIT 0.5%, mancozeb 50%, other components are the same as in example 2.

Example 9: 25.1% BBIT-mancozeb water-dispersible granules.

BBIT 0.1%, mancozeb 25%, other components are the same as in example 3.

(II) Preparation and examples of suspensions.

Active components A and B are uniformly mixed in proportion with dispersing agent, wetting agent, thickener, water and other components and milled and/or subjected to high shear to obtain an intermediate, which is analyzed, supplemented with water, uniformly mixed and filtered to obtain the finished product.

1) Preparation of suspensions of active ingredient A (BIT) and active ingredient B.

Example 10: 4% BIT-mancozeb suspension.

BIT 1%, mancozeb 3%, xanthan gum 3%, bentonite 4%, magnesium aluminum silicate 2%, ethylene glycol 2%, sodium lignin sulfonate 7% and water up to 100%.

Example 11: 15.1% BIT-mancozeb suspension.

BIT 0.1%, mancozeb 15%, bentonite 4%, glycerin 3%, sodium methylnaphthalenesulfonate condensate with formaldehyde 5% and water up to 100%.

Example 12: 21% BIT-mancozeb suspension.

BIT 1%, mancozeb 20%, white carbon 3%, glycerin 6%, sodium benzoate 2%, fatty alcohol ether phosphate with polyoxyethylene 7% and water up to 100%.

Example 13: 5% BIT-mancozeb suspension.

BIT 1%, mancozeb 4%, %, white carbon 4%, ethylene glycol 5%, sodium lignin sulfonate 7% xanthan gum 2% and water up to 100%.

2) Preparation of suspensions of active ingredient A (MBIT) and active ingredient B.

Example 14 4% MBIT-mancozeb suspension.

MBIT 1%, mancozeb 3%, other components are the same as in example 10.

Example 15: 15.1% MBIT-mancozeb suspension.

MBIT 0.1%, mancozeb 15%, other components are the same as in example 11.

Example 16: 21% MBIT-mancozeb suspension.

MBIT 1%, mancozeb 20%, other components are the same as in example 12.

Example 17: 5% MBIT-mancozeb suspension.

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MBIT 1%, mancozeb 4%, other components are the same as in example 13.

3) Preparation of suspensions of active ingredient A (BBIT) and active ingredient B.

Example 18: 4% BBIT-mancozeb suspension.

BBIT 1%, mancozeb 3%, other components are the same as in example 10.

Example 19: Suspension of 15.1% BBIT-mancozeb.

BBIT 0.1%, mancozeb 15%, other components are the same as in example 11.

Example 20: 21% BBIT-mancozeb suspension.

BBIT 1%, mancozeb 20%, other components are the same as in example 12.

Example 21: 5% BBIT-mancozeb suspension.

BBIT 1%, mancozeb 4%, other components are the same as in example 13.

(III) Preparation and examples of wettable powder.

Active components A and B and various additives and excipients are completely mixed in the ratio and ground in an ultrafine grinding apparatus to obtain a wettable powder.

1) Preparation of wettable powders of Active Ingredient A (BIT) and Active Ingredient B.

Example 22: Wettable powder 60.2% BIT-mancozeb (1:300).

BIT 0.2%, mancozeb 60%, sodium dodecylbenzenesulfonate 2%, calcium lignosulphate 3%, bentonite 3% and attapulgite up to 100%.

Example 23: Wettable powder 40.2% BIT-mancozeb (1:200).

BIT 0.2%, mancozeb 40%, Nekal 2%, bentonite 1.5%, alkyl polyoxyethylene ether sulfonate 1%, white carbon 2% and diatomaceous earth up to 100%.

Example 24: Wettable powder 51% BIT-mancozeb (1:50).

BIT 1%, mancozeb 50%, sodium lignin sulfonate 6%, alkyl sulfonate 7%, white carbon 10% and kaolin up to 100%.

2) Preparation of wettable powders of Active Ingredient A (MBIT) and Active Ingredient B.

Example 25: Wettable powder 60.2% MBIT-mancozeb.

MBIT 0.2%, mancozeb 60%, other components are the same as in example 22.

Example 26 40.2% MBIT Mancozeb Wettable Powder.

MBIT 0.2%, mancozeb 40%, other components are the same as in example 23.

Example 27 Wettable Powder 51% MBIT Mancozeb.

MBIT 1%, mancozeb 50%, other components are the same as in example 24.

3) Preparation of wettable powders of Active Ingredient A (BBIT) and Active Ingredient B.

Example 28 Wettable Powder 60.2% BBIT Mancozeb.

BBIT 0.2%, mancozeb 60%, other components are the same as in example 22.

Example 29 40.2% MBIT Mancozeb Wettable Powder.

BBIT 0.2%, mancozeb 40%, other components are the same as in example 23.

Example 30: Wettable powder 51% MBIT-mancozeb.

BBIT 1%, mancozeb 50%, other components are the same as in example 24.

II. Study to test effectiveness.

(I) Examples of bioassays.

Based on the grading scale, studies study the development of the disease on the leaves of the whole cucumber plant and calculate the disease index and the spread control effect.

Spread control effect is converted to probability (y), concentration of agents (µg/mL) in solution is converted to logarithmic value (x), toxicity regression equation and median inhibition concentration EC ₅₀ are calculated by least squares and toxicity index and cotoxicity ratio (CTC) of agents calculated by Pei Yong Sun's method.

Actual Toxicity Index (ATI)=(EC5 ₀ Standard/EC5 ₀ Investigational Agent)-100.

Theoretical Toxicity Index (TTI)=Agent Toxicity Index A-percentage of A in the mixture+Agent Toxicity Index B-percentage of B in the mixture.

Cotoxicity Index (CTC)=[Actual Toxicity Index (ATI) of the mixture/Theoretical Toxicity Index (TTI) of the mixture]*100.

When CTC>80 the composition exhibits an antagonistic effect; when 80<CTC<120 the composition exhibits an additive effect and when CTC>120 the composition exhibits a synergistic effect.

1) Toxicity study of BIT in combination with mancozeb.

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Table 1. Analysis of the results of the BIT study in combination with mancozeb on cucumber downy mildew

Agent name EC50 (mcg/ml) ATI TTI Sotoxicity coefficient (CTC) bit 13.5 100.00 / / Mancozeb 75.17 17.96 / / B1T: mancozeb = 1:300 60.79 22.21 18.233 121.81 BIT: mancozeb = 1:250 58.32 23.15 18.287 126.58 BIT: mancozeb = 1:200 56.70 23.81 18.368 129.62 BIT: mancozeb = 1:150 54.14 24.94 18.503 134.76 BIT: mancozeb = 1:100 50.88 26.53 18.772 141.35 BIT: mancozeb =1:75 50.41 26.78 18.233 146.87 BIT: mancozeb =1:50 48.29 27.95 19.569 142.82 BIT: mancozeb = 1:20 46.56 28.99 21.867 132.59 BIT: mancozeb =1:10 41.88 32.24 25.418 126.83 BIT: mancozeb = 1:4 31.70 42.59 34.368 123.91 BIT: mancozeb = 1:3 29.06 46.45 38.470 120.75 BIT: mancozeb = 1:2 25.70 52.52 45.307 115.93

The results (in Table 1) show that the spread control effect of the combination of BIT and Mancozeb on cucumber downy mildew is significantly improved, suggesting a marked synergistic effect in controlling the spread of cucumber downy mildew with the combination of the two components.

2) Toxicity study of MBIT in combination with mancozeb.

Table 2. Analysis of the results of the BIT study in combination. with mancozeb on late blight. potatoes.

Agent Name EC50 (mcg/ml) ATI TTI Sotoxicity coefficient (CTC) MBIT 14.81 100.00 / / Mancozeb 68.27 21.69 / / MBIT: mancozeb = 1:300 54.53 27.16 21,950 123.740 MBIT: mancozeb = 1:250 51.61 28.69 22.002 130.414 MBIT: mancozeb = 1:200 50.28 29.45 22.080 133.393 MBIT: mancozeb = 1:150 48.17 30.74 22.209 138.431 MBIT: mancozeb = 1:100 45.50 32.55 22.465 144.889 MBIT: mancozeb =1:75 45.50 32.55 21,950 148.298 MBIT: mancozeb =1:50 44.48 33.30 23.225 143.359 MBIT: mancozeb =1:20 42.75 34.65 25.419 136.304 MBIT: mancozeb =1:10 39.34 37.64 28.809 130.659 MBIT: mancozeb =1:4 31.27 47.36 37.352 126.798 MBIT: mancozeb = 1:3 29.25 50.64 41.268 122.701 MBIT: mancozeb = 1:2 26.04 56.86 47.793 118.977

The results (in Table 2) show that the spread control effect of the combination of MBIT and mancozeb on potato late blight is significantly improved, suggesting a marked synergistic effect.

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3) Toxicity study of BBIT in combination with mancozeb.

Table 3. Analysis of the results of the BBIT study in combination with mancozeb for . anthracnose apples

Agent name EC50 (mcg/ml) ATI TTI Sotoxicity coefficient (CTC) BBIT 19.09 100.00 / / Mancozeb 92.36 20.67 / / BBIT: mancozeb = 1:300 75.22 25.38 20.934 121.237 BBIT: mancozeb = 1:250 75.22 26.43 20.986 125.959 BBIT: mancozeb = 1:200 68.16 28.01 21.065 132.969 BBIT: mancozeb =1:150 65.71 29.05 21.195 137.058 BBIT: mancozeb =1:100 61.97 30.81 21.455 143.583 BBIT: mancozeb = 1:75 62.44 30.57 20.934 146.048 BBIT: mancozeb =1:50 60.89 31.35 22.225 141.068 BBIT: mancozeb = 1:20 58.31 32.74 24.448 133.910 BBIT: mancozeb = 1:10 52.58 36.30 27.882 130.207 BBIT: mancozeb = 1:4 41.36 46.15 36.536 126.316 BBIT: mancozeb = 1:3 38.57 49.49 40.503 122.187 BBIT: mancozeb =1:2 34.22 55.79 47.113 118.415

The results (in Table 3) show that the spread control effect of the combination of BBIT and Mancozeb on apple anthracnose is significantly improved, suggesting a marked synergistic effect in controlling the spread of apple anthracnose from the combination of the two components.

II. Field study to test effectiveness.

Research method: at an early stage of the development of the disease, the first spray is performed immediately, and then the second application is carried out after 7 days. Each treatment covers 4 experimental plots of 20 square meters each. The development of the disease after application and 11 days after the second application is examined statistically. Samples are randomly collected from 5 sites of each test plot and 5 plants from each site are examined, determining the percentage of the area of disease spots on the leaves in relation to the area of the leaves of the whole plant and classified. The disease index and spread control effect are calculated.

„ / Number of leaves in each Typical value \

Ζ / I degree of development of the disease of the corresponding degree I

Disease index=---- ¹ ------------------------------------------ ------- ^L x 100

Total number 1 Peak value x highest grade leaf examined

Spread control effect (%)=

Disease index in the control * Disease index in the treated group before application group after application

Disease index in control group Disease index in treated group after application group before application

Estimated spread control effect (%)=X+Y-XY/100 (where X and Y represent the spread control effects for a single agent).

Classification scale:

grade 0: no disease spots;

Grade 1: number of disease spots per leaf < 5 with length < 1 cm;

grade 3: 6> number of disease spots per leaf <10 with some disease spots >1 cm long;

grade 5: 11> number of disease spots per leaf >25, some disease spots are extended, and the area of disease spots is 10-25% of the leaf area;

grade 7: the number of disease spots per leaf is <26, the disease spots are extended, and the area of the disease spots is 26-50% of the leaf area;

grade 9: disease spots are extended, the area of disease spots is more than 50% of the leaf area, or all leaves are wilted.

1) Field study of the efficacy of BIT in combination with mancozeb.

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Table 4. Spread control effect of BIT in combination with mancozeb on apple circle rot

Agent Quantity about (mg/kg) Disease index before application 11 days after the second application Disease index The control effect is widespread Vododispersgiru received granules 5% BIT 16.9 11.94 18.19 25.36 Water-dispersible granules 75% mancozeb 1183.1 9.74 8.93 55.08 Estimated spread control effect after mixing them - - - 66.47 Water dispersible granules 71% B1T "mancose b (VP: mancozeb= 1 :70) 1200 10.53 5.37 75.04 Vododispersgiru received granules 3% BIT I,9 10.24 15.93 23.78

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Example 2

Example 3

Water-dispersible granules 75% mancozeb 1188.1 11.76 10.27 57.23 Estimated spread control effect after mixing them - - - 67.40 Water dispersible granules 50.5% IT manco zeb (VP:mancozeb= 1:100) 1200 9.02 4.56 75.26 Vododispersgiru received granules 3% BIT 4.8 15.4 27.55 12.35 Water dispersible granules 75% mancozeb 1195.2 10.56 8.42 60.94 Estimated spread control effect after mixing them - - - 65.76

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Water-dispersible granules 25.1% B1T» mancozeb (W1G: mancozeb = 1:250) 1200 13.03 7.65 71.23 Suspension 5% bit 300 12.04 9.61 60.89 Suspension 40% Macnozeb 900 9.94 15.41 44.03 Estimated spread control effect after mixing them - - - 78.11 Suspension 4% B 1T'mancozeb (SHG: mancozeb = 1 3) 1200 10.56 5.15 84.12 Suspension 5% bit 8 15.23 24.42 21.45 Suspension 40% Macnozeb 1192 10.47 8.87 58.51 Estimated spread control effect after mixing them - - - 67.41

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Example

Example

Suspension 15.1% B1T-manco zeb (BIT: mancozeb= 1:150) 1200 11.21 6.01 73.75 Suspension 5% bit 57.1 14.45 17.66 40.12 Suspension 40% Macnozeb 1142.9 12.74 14.51 44.2 Estimated spread control effect after mixing them - - - 66.59 Suspension 21%B1T»mancose b (GGG:mancozeb= 1:20) 1200 15.23 8.84 71.56 Suspension 5% bit 240 10.45 11.04 48.23 Suspension 40% Macnozeb 960 10.57 11.36 47.35 Estimated spread control effect after mixing them - - - 72.74

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Example

Example 23

Suspension 5% Brmancozeb (BIT: mancozeb=1 :4) 1200 9.04 4.09 77.86 wetted powder 3% BIT 4 12.45 22.81 10.24 Wettable powder 80% mancozeb 1196 10 7.86 61.51 Estimated spread control effect after mixing them - - - 65.45 wetted powder 60.2% IT manco zeb (BIT: mancozeb= 1:300) 1200 10.54 6.45 70.02 wetted powder 3% BIT 6 15.23 25.42 18.25 Wettable powder 80% mancozeb 1194 15.45 12.58 60.12 Estimated spread control effect after mixing them - - - 67.40

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wetted powder 40.2% IT manco zeb (BIT: mancozeb= 1:200) 1200 16.34 8.90 73.33 wetted powder 3% BIT 23.5 14.96 21.31 30.23 wetted 80% mancozeb powder 1176.5 15.02 15.25 50.25 Estimated spread control effect after mixing them - - - 65.29 wetted powder 51% B1T" Mancose B (BIT: mancozeb= 1 :50) 1200 14.22 8.18 71.82 Water control - 12.34 25.19 -

The results (in Table 4) show that the spread control effect of the combination of BIT and Mancozeb on apple circle rot was significantly improved, confirming the marked synergistic effect of the combination of the two components in controlling the spread of apple circle rot.

2) Field study of the efficacy of MBIT in combination with mancozeb.

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Number

Prime R5

Prime

P4

Table 5. Spread control effect of MBIT in combination with mancozeb on tomato brown spot

Agent Quantity o (a.i. g/ha) Disease index before application 11 days after the second application Illness index Spread control effect Water-dispersible granules 5% MBIT 28.0 13.45 17.93 29.2 Water-dispersible granules 75% mancozeb 1972.0 12.51 11.03 53.16 Estimated spread control effect after mixing them - - - 66.84 Water dispersible granules 71% MBIT-mancozeb (MBT: mancozeb=1:70) 2000.0 15.67 71.42 Water-dispersible granules 3% MBIT 19.8 17.35 24.76 24.21 Water-dispersible granules 75% mancozeb 1980.2 16.34 13.77 55.23 Estimated spread control effect after mixing them - - - 66.07

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Water dispersible granules 50.5% Brmancozeb (MBIT:mancozeb=1:100) 2000 14.23 6.51 75.69 Prime rb Water-dispersible granules 3% MBIT 8.0 14.36 23.89 11.63 Water-dispersible granules 75% mancozeb 1992.0 16.77 12.59 60.12 Estimated spread control effect after mixing them - - - 64.76 Water-dispersible granules 25.1% B1T» mancozeb (MW 1T: mancozeb = 1:25 0) 2000 15.28 8.04 72.07 Example 14 Suspension 5% MBIT 500 17.25 10.36 68.09 Suspension 40% Macnozeb 1500 16.33 21.15 31.21 Estimated spread control effect after mixing them - - - 78.05 Suspension 4% in IT mancozeb (MBIT: mancozeb= 1:3) 2000 18.23 6.49 81.08 Suspension 5% MBIT 13.2 15.34 23.01 20.33

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Suspension 40% Macnozeb 1986.8 14.79 11.64 58.21 Estimated spread control effect after mixing them - - - 66.71 Suspension 15.1% MVGPmancozeb (MBIT: mancozeb = 1:15 0) 2000 15.97 7.44 75.25 Suspension 5% MBIT 95.2 18.34 19.21 44.35 Suspension 40% Macnozeb 1904.8 10.99 10.72 48.2 Estimated spread control effect after mixing them - - - 71.17 Suspension 21%MB1T»mancozeb (MW 1T: mancozeb= 1:20) 2000 14.34 6.64 75.39 Suspension 5% MBIT 400.0 16.34 11.31 63.23 Suspension 40% Macnozeb 1600.0 15.27 18.65 35.11 Estimated spread control effect after mixing them - - - 76.14 Suspension 5% VShmankozeb (MBIT: mancozeb= 1:4) 2000 17.45 5.28 83.92

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Prime R25 wetted powder 3% MBIT 6.6 Wettable powder 80% mancozeb 1993.4 Estimated spread control effect after mixing them - wetted powder 60.2% MVSHmancozeb (MV 1T: mancozeb = 1:30 0) 2000 Prime R26 wetted powder 3% MBIT 10.0 Wettable powder 80% mancozeb 1990.0 Estimated spread control effect after mixing them - wetted powder 51% MWSHmancozeb (MBIT: mancozeb= 1:20 0) 2000 Prime R27 wetted powder 3% MBIT 39.2 Wettable powder 80% mancozeb 1960.8 Estimated spread control effect after mixing them - wetted powder 51% MB1T "mancozeb (MEP: mancozeb \u003d 1:50 0) 2000 SC water control -

17.35 29.62 9.31 15.94 11.71 60.98 - - 64.61 17.34 9.52 70.84 15.88 26.17 12.46 16.09 12.20 59.74 - - 64.76 13.45 6.90 72.75 14.78 15.48 44.35 18.26 20.21 41.20 - - 67.28 15.98 7.71 74.39 15.42 29.03 -

The results (in Table 5) show that the spread control effect of the combination of MBIT and Mancozeb on tomato brown spot is significantly improved, confirming the marked synergistic effect of the combination of the two components in controlling the spread of tomato brown spot.

3) Field study of the effectiveness of BBIT in combination with mancozeb.

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Table 6 Spread control effect of MBIT in combination with mancozeb on pear blackspot

Agent Quantity o (a.i. g/ha) Disease index before application 11 days after the second application Disease index I Control effect common IA Water-dispersible granules 5% BBIT 21 10.35 14.86 28.23 Water-dispersible granules 75% mancozeb 1479 9.04 8.27 54.24 Estimated spread control effect after mixing them - - - 67.16 Water dispersible granules 71% VVGVmancozeb (VV1T: mancozeb = 1:70) 1500 10.98 6.36 71.02 Water-dispersible granules 3% BBIT 14.9 13.2 19.74 25.22 Water-dispersible granules 75% mancozeb 1485.1 10.4 9.06 56.44 Estimated spread control effect after mixing them - - - 67.43 Water dispersible granules 50.5% VVGPmancozeb (BB 1T: mancozeb = 1:10 0) 1500 9.99 4.55 77.21 Water-dispersible granules 3% BBIT 6.0 11.11 19.53 12.11 Water-dispersible granules 75% mancozeb 1494.0 10.89 8.94 58.94

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Prime R9

Example 19

Estimated spread control effect after mixing them - - - 63.91 Water dispersible granules 25.1% WWGPmancozeb (BBIT: mancozeb = 1:25 0) 1500 12.39 6.97 71.88 Suspension 5% BBIT 375 15.23 11.44 62.45 Suspension 40% mancozeb 1125 10.93 14.52 33.59 Estimated spread control effect after mixing them - - - 75.06 Suspension 4% VVGPmankozeb (BBIT: mancozeb= 1:3) 1500 12.32 4.35 82.34 Suspension 5% BBIT 9.9 10.66 17.10 19.78 Suspension 40% mancozeb 1490.1 10.99 9.45 57.01 Estimated spread control effect after mixing them - - - 65.51 Suspension 15.1% BBIT mancozeb (BBIT: mancozeb = 1:150) 1500 15.24 7.66 74.85 Suspension 5% BBIT 71.4 15.23 16.71 45.14

- 19 040154

Example 20

Example 28

Suspension 40% mancozeb 1428.6 9.78 11.60 40.67 Estimated spread control effect after mixing them - - - 67.45 Suspension 21% VVGPmankozeb (BBIT: mancozeb= 1:20) 1500 12.45 6.48 73.99 Suspension 5% BBIT 300 9.68 9.45 51.21 Suspension 40% mancozeb 1200 10.64 13.39 37.08 Estimated spread control effect after mixing them - - - 69.30 Suspension 5% BB1T "mancozeb (BBIT: mancozeb= 1:4) 1500 11.23 5.35 76.18 wetted powder 3% BBIT 5.0 13.8 24.92 9.72 Wettable powder 80% mancozeb 1495.0 15.23 12.26 59.76 Estimated spread control effect after mixing them - - - 63.67

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wetted powder 60.2% B B IT mancozeb (BBIT: mancozeb = 1:30 0) 1500 9.78 5.63 71.20 Example 29 wetted powder 3% BBIT 7.5 10.38 17.79 14.32 Wettable powder 80% mancozeb 1492.5 11.45 9.72 57.57 Estimated spread control effect after mixing them - - - 63.65 wetted powder 40.2% B B IT mancozeb (GDP: mancozeb= 1:20 0) 1500 9.78 5.44 72.21 Prime RZO wetted powder 3% BBIT 29.4 12.33 14.66 40.56 Wettable powder 80% mancozeb 1470.6 10.44 11.44 45.21 Estimated spread control effect after mixing them - - - 67.43 wetted powder 51%BB IT mancozeb (GDP: mancozeb= 1:50) 1500 15.01 7.08 76.42 SC water control - 11.21 22.42 -

The results (in Table 5) show that the spread control effect of the combination of BBIT and Mancozeb on pear black spot is significantly improved, confirming the marked synergistic effect of the combination of the two components in controlling the spread of pear black spot.

Claims (8)

CLAIM 1. Fungicidal composition comprising active components A and B, consisting of two active components A and B, where the active component A is 1,2-benzisothiazolin-3-one, 2-methyl-1,2benzisothiazolin-3-one or 2 -n-butyl-1,2-benzisothiazolin-3-one, and active component B is mancozeb, and the weight ratio between the two components is 1:(3-300). 2. The fungicidal composition according to claim 1, characterized in that the mass ratio of the active component A to the active component B is 1:(4-280). 3. Fungicidal composition according to claim 2, characterized in that the mass ratio of the active component A to the active component B is 1:(10-260). 4. Fungicidal composition according to any one of pi. 1-3, characterized in that it is part of a pesticidally acceptable composition, additionally containing a pesticidal adjuvant or excipient. 5. Fungicidal composition according to claim 4, characterized in that the mass content of active components is 2-95%. 6. A fungicidal composition according to claim 4, characterized in that the composition is a wettable powder, suspension or water-dispersible granules. 7. Fungicidal composition according to claim 4, characterized in that the pesticidal adjuvant or auxiliary -21 040154 The body substance is one or more selected from the group consisting of carriers, solvent, dispersing agent, wetting agent, binder, thickener, adhesive, surfactant and fertilizer. 8. The use of a fungicidal composition according to any one of claims 1 to 7 for controlling the spread of crop diseases in agriculture.