EA033285B1 - Варианты белков axmi205 и способы их применения - Google Patents

Варианты белков axmi205 и способы их применения

Info

Publication number
EA033285B1
EA033285B1 EA201490379A EA201490379A EA033285B1 EA 033285 B1 EA033285 B1 EA 033285B1 EA 201490379 A EA201490379 A EA 201490379A EA 201490379 A EA201490379 A EA 201490379A EA 033285 B1 EA033285 B1 EA 033285B1
Authority
EA
Eurasian Patent Office
Prior art keywords
plants
compositions
bacteria
methods
seeds
Prior art date
Application number
EA201490379A
Other languages
English (en)
Other versions
EA201490379A1 (ru
Inventor
Фолькер Хайнрихс
Джейм Уилльямс
Original Assignee
Атеникс Корп.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Атеникс Корп. filed Critical Атеникс Корп.
Publication of EA201490379A1 publication Critical patent/EA201490379A1/ru
Publication of EA033285B1 publication Critical patent/EA033285B1/ru

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46N-acyl derivatives
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Insects & Arthropods (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Immunology (AREA)

Abstract

Обеспечиваются композиции и способы для придания пестицидной активности бактериям, растениям, растительным клеткам, тканям и семенам. Обеспечиваются композиции, содержащие кодирующие последовательности для пестицидных полипептидов. Кодирующие последовательности можно применять в ДНК-конструктах или экспрессионных кассетах для трансформации и экспрессии в растениях и бактериях. Композиции также содержат трансформированные бактерии, растения, растительные клетки, ткани и семена. В частности, обеспечиваются молекулы выделенных нуклеиновых кислот, кодирующих пестицид. Кроме того, охвачены аминокислотные последовательности, соответствующие полинуклеотидам. В частности, настоящее изобретение обеспечивает молекулы нуклеиновых кислот, содержащие нуклеотидные последовательности, кодирующие аминокислотную последовательность, показанную в SEQ ID NO: 7, 8, 9, 10, 11 или 12, нуклеотидную последовательность, приведенную под SEQ ID NO: 4, 5 или 6, а также их варианты и фрагменты.
EA201490379A 2011-07-28 2012-07-27 Варианты белков axmi205 и способы их применения EA033285B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161512539P 2011-07-28 2011-07-28
PCT/US2012/048496 WO2013016617A1 (en) 2011-07-28 2012-07-27 Axmi205 variant proteins and methods for their use

Publications (2)

Publication Number Publication Date
EA201490379A1 EA201490379A1 (ru) 2014-11-28
EA033285B1 true EA033285B1 (ru) 2019-09-30

Family

ID=46690701

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201490379A EA033285B1 (ru) 2011-07-28 2012-07-27 Варианты белков axmi205 и способы их применения

Country Status (15)

Country Link
US (2) US9862965B2 (ru)
EP (1) EP2737069B1 (ru)
CN (1) CN103975066B (ru)
AR (1) AR088746A1 (ru)
BR (1) BR112014001978B8 (ru)
CA (1) CA2842530C (ru)
EA (1) EA033285B1 (ru)
ES (1) ES2647596T3 (ru)
HU (1) HUE037346T2 (ru)
MX (1) MX351526B (ru)
PL (1) PL2737069T3 (ru)
RS (1) RS56642B1 (ru)
UA (1) UA122114C2 (ru)
WO (1) WO2013016617A1 (ru)
ZA (1) ZA201400562B (ru)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2014001070A (es) * 2011-07-29 2014-04-14 Athenix Corp Gen plaguicida axmi279 y sus metodos de uso.
EP2971000A4 (en) 2013-03-15 2016-11-23 Pioneer Hi Bred Int PHI-4 POLYPEPTIDES AND METHOD FOR THEIR USE
US9394345B2 (en) 2013-03-15 2016-07-19 Pioneer Hi-Bred International, Inc. PHI-4 polypeptides and methods for their use
UA123036C2 (uk) 2014-10-16 2021-02-03 Монсанто Текнолоджі Ллс Сконструйований інсектицидний білок, який має активність проти лускокрилих
PE20220372A1 (es) 2014-10-16 2022-03-16 Monsanto Technology Llc Proteinas quimericas insecticidas novedosas toxicas o inhibidoras de plagas de lepidopteros
US10487123B2 (en) 2014-10-16 2019-11-26 Monsanto Technology Llc Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests
US10316329B2 (en) 2014-10-16 2019-06-11 Monsanto Technology Llc Proteins toxic or inhibitory to lepidopteran insects
CN109475096B (zh) 2015-08-06 2022-08-23 先锋国际良种公司 植物来源的杀昆虫蛋白及其使用方法
CN109475124B (zh) 2015-08-27 2021-11-19 孟山都技术公司 新型昆虫抑制蛋白
CA3076020A1 (en) 2017-10-02 2019-04-11 Syngenta Participations Ag Engineered pesticidal proteins and methods of controlling plant pests
WO2022125639A1 (en) 2020-12-08 2022-06-16 Monsanto Technology Llc Modified plant-associated bacteria and methods of their use
AR124445A1 (es) 2020-12-21 2023-03-29 Monsanto Technology Llc Proteínas inhibidoras de insectos novedosas
UY39585A (es) 2020-12-23 2022-07-29 Monsanto Technology Llc Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas
EP4271188A1 (en) 2020-12-31 2023-11-08 Monsanto Technology LLC Novel insect inhibitory proteins
CA3226147A1 (en) 2021-07-08 2023-01-12 Monsanto Technology Llc Novel insect inhibitory proteins

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007147096A2 (en) * 2006-06-15 2007-12-21 Athenix Corporation A family of pesticidal proteins and methods for their use
WO2010091230A1 (en) * 2009-02-05 2010-08-12 Athenix Corporation Variant axmi-r1 delta-endotoxin genes and methods for their use
WO2010141141A2 (en) * 2009-03-11 2010-12-09 Athenix Corporation Axmi-001, axmi-002, axmi-030, axmi-035, and axmi-045: toxin genes and methods for their use
WO2011002992A1 (en) * 2009-07-02 2011-01-06 Athenix Corp. Axmi-205 pesticidal gene and methods for its use

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4196265A (en) 1977-06-15 1980-04-01 The Wistar Institute Method of producing antibodies
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5039523A (en) 1988-10-27 1991-08-13 Mycogen Corporation Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
EP0482125A1 (en) 1989-07-11 1992-04-29 Biotechnology Research And Development Corporation Aerosol beam microinjection
US5240842A (en) 1989-07-11 1993-08-31 Biotechnology Research And Development Corporation Aerosol beam microinjector
CA2051562C (en) 1990-10-12 2003-12-02 Jewel M. Payne Bacillus thuringiensis isolates active against dipteran pests
TW261517B (ru) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5743477A (en) 1992-08-27 1998-04-28 Dowelanco Insecticidal proteins and method for plant protection
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6060594A (en) * 1997-12-18 2000-05-09 Ecogen, Inc. Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins
US6468523B1 (en) 1998-11-02 2002-10-22 Monsanto Technology Llc Polypeptide compositions toxic to diabrotic insects, and methods of use
US6938976B2 (en) 1999-06-16 2005-09-06 Eastman Kodak Company Printer and method therefor adapted to sense data uniquely associated with a consumable loaded into the printer
AU7461900A (en) 1999-09-02 2001-03-26 Agresearch Limited Nucleotide sequences
AU1803601A (en) 1999-11-29 2001-06-04 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
US20050183161A1 (en) 2003-10-14 2005-08-18 Athenix Corporation AXMI-010, a delta-endotoxin gene and methods for its use
CN100532555C (zh) * 2004-11-16 2009-08-26 中国农业科学院植物保护研究所 对鞘翅目害虫高效的cry8F基因、其表达蛋白及其应用
CN100999718B (zh) * 2006-07-06 2011-06-15 湖南师范大学 几丁质酶基因重组的苏云金杆菌工程菌
EP2201030A2 (en) 2007-10-16 2010-06-30 Athenix Corporation Axmi-066 and axmi-076: delta-endotoxin proteins and methods for their use
MX2011008955A (es) 2009-02-27 2011-09-30 Athenix Corp Proteinas pesticidas y metodos de uso de las mismas.
WO2012038480A2 (en) * 2010-09-22 2012-03-29 Bayer Cropscience Ag Use of biological or chemical control agents for controlling insects and nematodes in resistant crops

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007147096A2 (en) * 2006-06-15 2007-12-21 Athenix Corporation A family of pesticidal proteins and methods for their use
WO2010091230A1 (en) * 2009-02-05 2010-08-12 Athenix Corporation Variant axmi-r1 delta-endotoxin genes and methods for their use
WO2010141141A2 (en) * 2009-03-11 2010-12-09 Athenix Corporation Axmi-001, axmi-002, axmi-030, axmi-035, and axmi-045: toxin genes and methods for their use
WO2011002992A1 (en) * 2009-07-02 2011-01-06 Athenix Corp. Axmi-205 pesticidal gene and methods for its use

Also Published As

Publication number Publication date
HUE037346T2 (hu) 2018-08-28
EA201490379A1 (ru) 2014-11-28
EP2737069B1 (en) 2017-08-23
US9862965B2 (en) 2018-01-09
MX2014001069A (es) 2014-04-14
US20180080039A1 (en) 2018-03-22
BR112014001978B1 (pt) 2021-06-15
US10875897B2 (en) 2020-12-29
CA2842530A1 (en) 2013-01-31
AR088746A1 (es) 2014-07-02
RS56642B1 (sr) 2018-03-30
MX351526B (es) 2017-10-18
UA122114C2 (uk) 2020-09-25
ZA201400562B (en) 2017-06-28
ES2647596T3 (es) 2017-12-22
CA2842530C (en) 2020-11-10
PL2737069T3 (pl) 2018-01-31
BR112014001978A2 (pt) 2017-02-21
WO2013016617A1 (en) 2013-01-31
US20140298538A1 (en) 2014-10-02
CN103975066A (zh) 2014-08-06
EP2737069A1 (en) 2014-06-04
CN103975066B (zh) 2018-03-27
BR112014001978B8 (pt) 2022-07-05

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