EA033285B1 - Варианты белков axmi205 и способы их применения - Google Patents
Варианты белков axmi205 и способы их примененияInfo
- Publication number
- EA033285B1 EA033285B1 EA201490379A EA201490379A EA033285B1 EA 033285 B1 EA033285 B1 EA 033285B1 EA 201490379 A EA201490379 A EA 201490379A EA 201490379 A EA201490379 A EA 201490379A EA 033285 B1 EA033285 B1 EA 033285B1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- plants
- compositions
- bacteria
- methods
- seeds
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 241000894006 Bacteria Species 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 3
- 230000000361 pesticidal effect Effects 0.000 abstract 3
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 108091026890 Coding region Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Insects & Arthropods (AREA)
- Mycology (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Immunology (AREA)
Abstract
Обеспечиваются композиции и способы для придания пестицидной активности бактериям, растениям, растительным клеткам, тканям и семенам. Обеспечиваются композиции, содержащие кодирующие последовательности для пестицидных полипептидов. Кодирующие последовательности можно применять в ДНК-конструктах или экспрессионных кассетах для трансформации и экспрессии в растениях и бактериях. Композиции также содержат трансформированные бактерии, растения, растительные клетки, ткани и семена. В частности, обеспечиваются молекулы выделенных нуклеиновых кислот, кодирующих пестицид. Кроме того, охвачены аминокислотные последовательности, соответствующие полинуклеотидам. В частности, настоящее изобретение обеспечивает молекулы нуклеиновых кислот, содержащие нуклеотидные последовательности, кодирующие аминокислотную последовательность, показанную в SEQ ID NO: 7, 8, 9, 10, 11 или 12, нуклеотидную последовательность, приведенную под SEQ ID NO: 4, 5 или 6, а также их варианты и фрагменты.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161512539P | 2011-07-28 | 2011-07-28 | |
PCT/US2012/048496 WO2013016617A1 (en) | 2011-07-28 | 2012-07-27 | Axmi205 variant proteins and methods for their use |
Publications (2)
Publication Number | Publication Date |
---|---|
EA201490379A1 EA201490379A1 (ru) | 2014-11-28 |
EA033285B1 true EA033285B1 (ru) | 2019-09-30 |
Family
ID=46690701
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201490379A EA033285B1 (ru) | 2011-07-28 | 2012-07-27 | Варианты белков axmi205 и способы их применения |
Country Status (15)
Country | Link |
---|---|
US (2) | US9862965B2 (ru) |
EP (1) | EP2737069B1 (ru) |
CN (1) | CN103975066B (ru) |
AR (1) | AR088746A1 (ru) |
BR (1) | BR112014001978B8 (ru) |
CA (1) | CA2842530C (ru) |
EA (1) | EA033285B1 (ru) |
ES (1) | ES2647596T3 (ru) |
HU (1) | HUE037346T2 (ru) |
MX (1) | MX351526B (ru) |
PL (1) | PL2737069T3 (ru) |
RS (1) | RS56642B1 (ru) |
UA (1) | UA122114C2 (ru) |
WO (1) | WO2013016617A1 (ru) |
ZA (1) | ZA201400562B (ru) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MX2014001070A (es) * | 2011-07-29 | 2014-04-14 | Athenix Corp | Gen plaguicida axmi279 y sus metodos de uso. |
EP2971000A4 (en) | 2013-03-15 | 2016-11-23 | Pioneer Hi Bred Int | PHI-4 POLYPEPTIDES AND METHOD FOR THEIR USE |
US9394345B2 (en) | 2013-03-15 | 2016-07-19 | Pioneer Hi-Bred International, Inc. | PHI-4 polypeptides and methods for their use |
UA123036C2 (uk) | 2014-10-16 | 2021-02-03 | Монсанто Текнолоджі Ллс | Сконструйований інсектицидний білок, який має активність проти лускокрилих |
PE20220372A1 (es) | 2014-10-16 | 2022-03-16 | Monsanto Technology Llc | Proteinas quimericas insecticidas novedosas toxicas o inhibidoras de plagas de lepidopteros |
US10487123B2 (en) | 2014-10-16 | 2019-11-26 | Monsanto Technology Llc | Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests |
US10316329B2 (en) | 2014-10-16 | 2019-06-11 | Monsanto Technology Llc | Proteins toxic or inhibitory to lepidopteran insects |
CN109475096B (zh) | 2015-08-06 | 2022-08-23 | 先锋国际良种公司 | 植物来源的杀昆虫蛋白及其使用方法 |
CN109475124B (zh) | 2015-08-27 | 2021-11-19 | 孟山都技术公司 | 新型昆虫抑制蛋白 |
CA3076020A1 (en) | 2017-10-02 | 2019-04-11 | Syngenta Participations Ag | Engineered pesticidal proteins and methods of controlling plant pests |
WO2022125639A1 (en) | 2020-12-08 | 2022-06-16 | Monsanto Technology Llc | Modified plant-associated bacteria and methods of their use |
AR124445A1 (es) | 2020-12-21 | 2023-03-29 | Monsanto Technology Llc | Proteínas inhibidoras de insectos novedosas |
UY39585A (es) | 2020-12-23 | 2022-07-29 | Monsanto Technology Llc | Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas |
EP4271188A1 (en) | 2020-12-31 | 2023-11-08 | Monsanto Technology LLC | Novel insect inhibitory proteins |
CA3226147A1 (en) | 2021-07-08 | 2023-01-12 | Monsanto Technology Llc | Novel insect inhibitory proteins |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007147096A2 (en) * | 2006-06-15 | 2007-12-21 | Athenix Corporation | A family of pesticidal proteins and methods for their use |
WO2010091230A1 (en) * | 2009-02-05 | 2010-08-12 | Athenix Corporation | Variant axmi-r1 delta-endotoxin genes and methods for their use |
WO2010141141A2 (en) * | 2009-03-11 | 2010-12-09 | Athenix Corporation | Axmi-001, axmi-002, axmi-030, axmi-035, and axmi-045: toxin genes and methods for their use |
WO2011002992A1 (en) * | 2009-07-02 | 2011-01-06 | Athenix Corp. | Axmi-205 pesticidal gene and methods for its use |
Family Cites Families (22)
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US4196265A (en) | 1977-06-15 | 1980-04-01 | The Wistar Institute | Method of producing antibodies |
US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
US5039523A (en) | 1988-10-27 | 1991-08-13 | Mycogen Corporation | Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin |
EP0482125A1 (en) | 1989-07-11 | 1992-04-29 | Biotechnology Research And Development Corporation | Aerosol beam microinjection |
US5240842A (en) | 1989-07-11 | 1993-08-31 | Biotechnology Research And Development Corporation | Aerosol beam microinjector |
CA2051562C (en) | 1990-10-12 | 2003-12-02 | Jewel M. Payne | Bacillus thuringiensis isolates active against dipteran pests |
TW261517B (ru) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
US5743477A (en) | 1992-08-27 | 1998-04-28 | Dowelanco | Insecticidal proteins and method for plant protection |
US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6060594A (en) * | 1997-12-18 | 2000-05-09 | Ecogen, Inc. | Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins |
US6468523B1 (en) | 1998-11-02 | 2002-10-22 | Monsanto Technology Llc | Polypeptide compositions toxic to diabrotic insects, and methods of use |
US6938976B2 (en) | 1999-06-16 | 2005-09-06 | Eastman Kodak Company | Printer and method therefor adapted to sense data uniquely associated with a consumable loaded into the printer |
AU7461900A (en) | 1999-09-02 | 2001-03-26 | Agresearch Limited | Nucleotide sequences |
AU1803601A (en) | 1999-11-29 | 2001-06-04 | Midwest Oilseeds, Inc. | Methods and compositions for the introduction of molecules into cells |
US20050183161A1 (en) | 2003-10-14 | 2005-08-18 | Athenix Corporation | AXMI-010, a delta-endotoxin gene and methods for its use |
CN100532555C (zh) * | 2004-11-16 | 2009-08-26 | 中国农业科学院植物保护研究所 | 对鞘翅目害虫高效的cry8F基因、其表达蛋白及其应用 |
CN100999718B (zh) * | 2006-07-06 | 2011-06-15 | 湖南师范大学 | 几丁质酶基因重组的苏云金杆菌工程菌 |
EP2201030A2 (en) | 2007-10-16 | 2010-06-30 | Athenix Corporation | Axmi-066 and axmi-076: delta-endotoxin proteins and methods for their use |
MX2011008955A (es) | 2009-02-27 | 2011-09-30 | Athenix Corp | Proteinas pesticidas y metodos de uso de las mismas. |
WO2012038480A2 (en) * | 2010-09-22 | 2012-03-29 | Bayer Cropscience Ag | Use of biological or chemical control agents for controlling insects and nematodes in resistant crops |
-
2012
- 2012-07-27 US US14/233,426 patent/US9862965B2/en active Active
- 2012-07-27 AR ARP120102749A patent/AR088746A1/es active IP Right Grant
- 2012-07-27 RS RS20171201A patent/RS56642B1/sr unknown
- 2012-07-27 CN CN201280046408.1A patent/CN103975066B/zh active Active
- 2012-07-27 ES ES12748303.0T patent/ES2647596T3/es active Active
- 2012-07-27 UA UAA201402009A patent/UA122114C2/uk unknown
- 2012-07-27 HU HUE12748303A patent/HUE037346T2/hu unknown
- 2012-07-27 CA CA2842530A patent/CA2842530C/en active Active
- 2012-07-27 BR BR112014001978A patent/BR112014001978B8/pt active IP Right Grant
- 2012-07-27 WO PCT/US2012/048496 patent/WO2013016617A1/en active Application Filing
- 2012-07-27 PL PL12748303T patent/PL2737069T3/pl unknown
- 2012-07-27 EA EA201490379A patent/EA033285B1/ru not_active IP Right Cessation
- 2012-07-27 EP EP12748303.0A patent/EP2737069B1/en active Active
- 2012-07-27 MX MX2014001069A patent/MX351526B/es active IP Right Grant
-
2014
- 2014-01-23 ZA ZA2014/00562A patent/ZA201400562B/en unknown
-
2017
- 2017-11-30 US US15/826,743 patent/US10875897B2/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007147096A2 (en) * | 2006-06-15 | 2007-12-21 | Athenix Corporation | A family of pesticidal proteins and methods for their use |
WO2010091230A1 (en) * | 2009-02-05 | 2010-08-12 | Athenix Corporation | Variant axmi-r1 delta-endotoxin genes and methods for their use |
WO2010141141A2 (en) * | 2009-03-11 | 2010-12-09 | Athenix Corporation | Axmi-001, axmi-002, axmi-030, axmi-035, and axmi-045: toxin genes and methods for their use |
WO2011002992A1 (en) * | 2009-07-02 | 2011-01-06 | Athenix Corp. | Axmi-205 pesticidal gene and methods for its use |
Also Published As
Publication number | Publication date |
---|---|
HUE037346T2 (hu) | 2018-08-28 |
EA201490379A1 (ru) | 2014-11-28 |
EP2737069B1 (en) | 2017-08-23 |
US9862965B2 (en) | 2018-01-09 |
MX2014001069A (es) | 2014-04-14 |
US20180080039A1 (en) | 2018-03-22 |
BR112014001978B1 (pt) | 2021-06-15 |
US10875897B2 (en) | 2020-12-29 |
CA2842530A1 (en) | 2013-01-31 |
AR088746A1 (es) | 2014-07-02 |
RS56642B1 (sr) | 2018-03-30 |
MX351526B (es) | 2017-10-18 |
UA122114C2 (uk) | 2020-09-25 |
ZA201400562B (en) | 2017-06-28 |
ES2647596T3 (es) | 2017-12-22 |
CA2842530C (en) | 2020-11-10 |
PL2737069T3 (pl) | 2018-01-31 |
BR112014001978A2 (pt) | 2017-02-21 |
WO2013016617A1 (en) | 2013-01-31 |
US20140298538A1 (en) | 2014-10-02 |
CN103975066A (zh) | 2014-08-06 |
EP2737069A1 (en) | 2014-06-04 |
CN103975066B (zh) | 2018-03-27 |
BR112014001978B8 (pt) | 2022-07-05 |
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Designated state(s): AM AZ BY KZ KG TJ TM |