EA028538B1 - Способ изготовления рекомбинантной тирозиназы человека - Google Patents

Способ изготовления рекомбинантной тирозиназы человека Download PDF

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Publication number
EA028538B1
EA028538B1 EA201500174A EA201500174A EA028538B1 EA 028538 B1 EA028538 B1 EA 028538B1 EA 201500174 A EA201500174 A EA 201500174A EA 201500174 A EA201500174 A EA 201500174A EA 028538 B1 EA028538 B1 EA 028538B1
Authority
EA
Eurasian Patent Office
Prior art keywords
human tyrosinase
tyrosinase
gene
cell
cells
Prior art date
Application number
EA201500174A
Other languages
English (en)
Russian (ru)
Other versions
EA201500174A1 (ru
Inventor
Ганеш Чандрамоули
Самиран Махапатра
Шриниваса Тхиммаиах
Динешкумар Харибхау Дандекар
Ситха Кришнан
Original Assignee
Юнилевер Н.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Юнилевер Н.В. filed Critical Юнилевер Н.В.
Publication of EA201500174A1 publication Critical patent/EA201500174A1/ru
Publication of EA028538B1 publication Critical patent/EA028538B1/ru

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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0055Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0059Catechol oxidase (1.10.3.1), i.e. tyrosinase
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00Preparations for care of the hair
    • A61Q5/10Preparations for permanently dyeing the hair
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y110/00Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03001Catechol oxidase (1.10.3.1), i.e. tyrosinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/18Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with another compound as one donor, and incorporation of one atom of oxygen (1.14.18)
    • C12Y114/18001Tyrosinase (1.14.18.1)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/42Colour properties
    • A61K2800/43Pigments; Dyes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/592Mixtures of compounds complementing their respective functions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EA201500174A 2012-07-26 2013-07-10 Способ изготовления рекомбинантной тирозиназы человека EA028538B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12177945 2012-07-26
EP12184662 2012-09-17
PCT/EP2013/064555 WO2014016118A1 (en) 2012-07-26 2013-07-10 A method of preparing recombinant human tyrosinase

Publications (2)

Publication Number Publication Date
EA201500174A1 EA201500174A1 (ru) 2015-06-30
EA028538B1 true EA028538B1 (ru) 2017-11-30

Family

ID=48793219

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201500174A EA028538B1 (ru) 2012-07-26 2013-07-10 Способ изготовления рекомбинантной тирозиназы человека

Country Status (7)

Country Link
US (2) US20150232818A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP2877573B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (1) CN104662149B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AR (1) AR091904A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EA (1) EA028538B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IN (1) IN2015MN00102A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2014016118A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115025217B (zh) * 2022-05-13 2023-05-05 广东齐美医药生物科技集团有限公司 干细胞裂解物联合活性多糖以及酪氨酸酶抑制剂在制备药物或化妆品中的用途
CN117904149A (zh) * 2023-10-25 2024-04-19 中国医学科学院北京协和医院 一种表达酪氨酸酶的mRNA及用途

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990012869A1 (en) * 1989-04-26 1990-11-01 Sloan-Kettering Institute For Cancer Research Non-melanocytic, eucaryotic cell constitutively expressing biologically active human tyrosinase and use thereof
WO1994022468A1 (en) * 1993-04-02 1994-10-13 Anticancer, Inc. Method for delivering beneficial compositions to hair follicles
WO2011060351A2 (en) * 2009-11-13 2011-05-19 Warner Babcock Institute For Green Chemistry, Llc Coloring composition containing an aromatic compound and tyrosinase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4515773A (en) * 1983-07-05 1985-05-07 Repligen Corporation Skin tanning composition and method
US5773291A (en) * 1989-04-26 1998-06-30 Sloan-Kettering Institute For Cancer Research Non-melanotytic mammalian cell constitutively expressing biologically active human tyrosinase and use thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990012869A1 (en) * 1989-04-26 1990-11-01 Sloan-Kettering Institute For Cancer Research Non-melanocytic, eucaryotic cell constitutively expressing biologically active human tyrosinase and use thereof
WO1994022468A1 (en) * 1993-04-02 1994-10-13 Anticancer, Inc. Method for delivering beneficial compositions to hair follicles
WO2011060351A2 (en) * 2009-11-13 2011-05-19 Warner Babcock Institute For Green Chemistry, Llc Coloring composition containing an aromatic compound and tyrosinase

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ENDO KOHKI, ET AL: "Enzymological characterization of EpoA, a laccase-like phenol oxidase produced by Streptomyces griseus.", JOURNAL OF BIOCHEMISTRY, OXFORD UNIVERSITY PRESS, GB, vol. 133, no. 5, 1 May 2003 (2003-05-01), GB, pages 671 - 677, XP002387763, ISSN: 0021-924X, DOI: 10.1093/jb/mvg086 *
GEN-HUNG CHEN, WEI-MING CHEN, YA-CHI HUANG, SHANN-TZONG JIANG: "Expression of Recombinant Mature Human Tyrosinase from Escherichia coli and Exhibition of Its Activity without Phosphorylation or Glycosylation", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY, BOOKS AND JOURNALS DIVISION, vol. 60, no. 11, 1 March 2012 (2012-03-01), pages 2838 - 2843, XP002694899, ISSN: 0021-8561, DOI: 10.1021/JF205021G *
M B DOLINSKA, ET AL.: "Human modified tyrosinase and two temperature-sensitive mutants are soluble active enzymes", ARVO 2012 ABSTRACT SEARCH & ITINERARY BUILDER, 7 May 2012 (2012-05-07), pages 1 - 4, XP002688743, Retrieved from the Internet <URL:http://www.abstractsonline.com/Plan/ViewAbstract.aspx?mID=2866&sKey=856ee1ea-c6e8-4af7-9cd1-cd022e45ca70&cKey=ec46eea5-b466-4cfb-87ef-a530779522ef&mKey={F0FCE029-9BF8-4E7C-B48E-9FF7711D4A0E}> [retrieved on 20121205] *
MONIQUE M. VAN OERS;: "Opportunities and challenges for the baculovirus expression system", JOURNAL OF INVERTEBRATE PATHOLOGY, SAN DIEGO, CA, US, vol. 107, US, pages S3 - S15, XP028244442, ISSN: 0022-2011, DOI: 10.1016/j.jip.2011.05.001 *
W. LIU, Y. CHAO, S. LIU, H. BAO, S. QIAN: "Molecular cloning and characterization of a laccase gene from the basidiomycete Fome lignosus and expression in Pichia pastoris", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER-VERLAG, vol. 63, no. 2, 1 December 2003 (2003-12-01), pages 174 - 181, XP055075251, ISSN: 01757598, DOI: 10.1007/s00253-003-1398-0 *

Also Published As

Publication number Publication date
CN104662149B (zh) 2018-11-20
IN2015MN00102A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2015-10-16
EP2877573B1 (en) 2016-09-21
WO2014016118A1 (en) 2014-01-30
US20150232818A1 (en) 2015-08-20
AR091904A1 (es) 2015-03-11
CN104662149A (zh) 2015-05-27
US20160324751A1 (en) 2016-11-10
EP2877573A1 (en) 2015-06-03
EA201500174A1 (ru) 2015-06-30

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