EA021467B1 - Bacterial fertilizer and method of its preparing - Google Patents

Bacterial fertilizer and method of its preparing Download PDF

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Publication number
EA021467B1
EA021467B1 EA201100400A EA201100400A EA021467B1 EA 021467 B1 EA021467 B1 EA 021467B1 EA 201100400 A EA201100400 A EA 201100400A EA 201100400 A EA201100400 A EA 201100400A EA 021467 B1 EA021467 B1 EA 021467B1
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EA
Eurasian Patent Office
Prior art keywords
cells
sorbent
bacteria
vasshik
strains
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EA201100400A
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Russian (ru)
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EA201100400A1 (en
Inventor
Кахрамон Давранович Давранов
Original Assignee
Кахрамон Давранович Давранов
Товарищество С Ограниченной Ответственностью "New Tec Group"
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Publication of EA201100400A1 publication Critical patent/EA201100400A1/en
Publication of EA021467B1 publication Critical patent/EA021467B1/en

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  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to agriculture. The invention is aimed at broadening the range of effective bacterial fertilizers having a stimulating action on plant growth, with wider action spectrum against phytopathogenic fungi including Verticillium species, with a prolonged storage and action period and at working out a method for preparing same. The invention is characterised in that the bacterial fertilizer comprises cells of Bacillus subtillis B-1, Bacillus megaterium BM-1, Azotobacter chroococcum A-2, Pseudomonas putida PP-1, Streptomyces atratus UZGIT-1 bacteria strains and a sorbent - a mixture of biohumus and carboxymethyl cellulose (CMC) taken in mass ratio 4:1 correspondingly, with component ratio given in the invention claims. The method comprises culturing cells of Bacillus subtillis B-1, Bacillus megaterium BM-1, Azotobacter chroococcum A-2, Pseudomonas putida PP-1, Streptomyces atratus UZGIT-1 bacteria strains, preparing the sorbent by mixing biohumus and CMC taken in mass ratio 4:1 correspondingly, mixing culture fluid obtained after culturing of bacteria strain cells and the sorbent and granulation.

Description

(57) Изобретение используется в сельском хозяйстве. Задача изобретения: расширение ассортимента эффективных бактериальных удобрений с широким спектром действия, обладающих стимулирующим действием на рост растений, более широким спектром действия против фитопатогенов, в том числе грибов рода УеШсШшш, с пролонгированным сроком хранения и действия, и разработка способа его получения. Сущность изобретения: бактериальное удобрение содержит клетки штаммов бактерий ВасШик киЬЫЬк В-1, ВасШик тсдаЮпшп ВМ-1, А/о1оЬас1сг сЬгоососсит А-2, Рксиботопак рШШа РР-1, ШгсрЮтусск айаШк υΖΟΙΤ-1 и сорбент - смесь биогумуса и КМЦ, взятых в массовом соотношении, равном 4:1 соответственно, при соотношении компонентов, указанном в формуле изобретения. Способ включает культивирование клеток штаммов ВасШик киЬШЬк В-1, ВасШик тсдаЮпит ВМ-1, А/о1оЬас1сг сЬгоососсит А-2, Рксиботопак риОйа РР-1, ШгсрЮтусск айаШк υΖΟΙΤ-1, приготовление сорбента смешиванием биогумуса и КМЦ, взятых в массовом соотношении, равном 4:1 соответственно, смешивание культуральных жидкостей, полученных после культивирования клеток штаммов бактерий, и сорбента, гранулирование.(57) The invention is used in agriculture. The objective of the invention: expanding the range of effective bacterial fertilizers with a wide spectrum of action, which have a stimulating effect on plant growth, a wider spectrum of action against phytopathogens, including fungi of the genus Uehnsh, with a prolonged shelf life and action, and the development of a method for its production. The inventive bacterial fertilizer contains cells of the bacterial strains Vasix, bacillus B-1, bacillus bacillus bacillus A-2, Rxibotopacus bacillus RR-1, Rhysbutus arbumus sorbent and sorbent mixture, sorbent and sorbent a mass ratio of 4: 1, respectively, with the ratio of components specified in the claims. SUBSTANCE: method involves culturing cells of the strains Vashikik bishk B-1, Vaschik tsdjupit BM-1, A / o1obac1cg bbossoss A-2, Rksibotopak riOiya PP-1, Shrcryutus aiabk vu-1, preparation of the sorbent by mixing, by mass mixing, 4: 1, respectively, mixing the culture fluids obtained after culturing the cells of bacterial strains, and sorbent, granulation.

Claims (2)

1. Бактериальное удобрение, содержащее клетки штаммов бактерий ВасШик, сорбент и остатки культуральной жидкости с метаболитами, отличающееся тем, что оно содержит клетки штаммов бактерий ВасШик киЫШк В-1 и ВасШик тсдЦспит ВМ-1 и дополнительно содержит клетки штаммов бактерий А/о1оЬас1сг сйгоососсит А-2, Ркеиботопак рибба РР-1, ЫгсрЮтусск абаШк υΖΟΙΤ-1, в качестве сорбента содержит смесь биогумуса и карбоксиметилцеллюлозу, взятых в массовом соотношении, равном (35):(0,5-1,5) соответственно, при следующем соотношении компонентов, на 1 г удобрения:1. Bacterial fertilizer containing cells of the bacteria strains VasShik, sorbent and the remains of the culture fluid with metabolites, characterized in that it contains cells of the bacteria strains VasShik kiyshk B-1 and VasShik tsdtspit VM-1 and additionally contains cells of the bacteria strains -2, Rkeybotopak ribba PP-1, Igrsrutussk abaShk υΖΟΙΤ-1, as a sorbent contains a mixture of biohumus and carboxymethyl cellulose taken in a mass ratio equal to (35) :( 0.5-1.5), respectively, with the following ratio of components, per 1 g of fertilizer: клетки штамма бактерийbacteria strain cells АгоюЬайег сйгоососсит А-2, млрд 0,5-1,5Agoyuayeg sygoosossit A-2, 0.5-1.5 billion Клетки штамма бактерийBacteria strain cells Ркеиботопак рибба РР-1, млн 75-150Rkeibotopack ribba PP-1, million 75-150 Клетки штамма бактерий $1гер(отусе5 аСгаШк υΖ6ΙΤ-1, млн 20-100Cells of a bacterial strain of $ 1 ger (otus5 aSgaShk υΖ6ΙΤ-1, mln Клетки штамма бактерийBacteria strain cells ВасШик те§а1епит ВМ-1, млн 20-150VasShik tega1epit VM-1, million 20-150 Клетки штамма бактерииBacteria strain cells ВасШик киЫШк В-1, млн 20-150VasShik kiyshk V-1, million 20-150 Указанный сорбент, г 0,2-0,3The specified sorbent, g 0.2-0.3 Остатки культуральной жидкости с метаболитами Остальное.Residues of culture fluid with metabolites Else. 2. Способ получения бактериального удобрения по п.1, включающий культивирование клеток штаммов бактерий ВасШик, приготовление сорбента, смешивание культуральных жидкостей, содержащих бактерии, и сорбента и гранулирование, отличающийся тем, что культивируют клетки штаммов бактерий ВасШик киЫШк В-1 и ВасШик тсдЦспит ВМ-1 и дополнительно А/о1оЬас1сг сйгоососсит А-2, Ркеиботопак рибба РР-1, 81гср1отусск абаШк υΖΟΙΤ-1, готовят сорбент смешиванием биогумуса с карбоксиметилцеллюлозой в массовом соотношении, равном (3-5):(0,5-1,5) соответственно, смешивают клетки указанных штаммов бактерий и остатки культивированной жидкости, содержащей метаболиты, с2. The method of producing the bacterial fertilizer according to claim 1, comprising cultivating cells of the bacteria strains VasShik, preparing the sorbent, mixing the culture fluids containing bacteria, and sorbent and granulating, characterized in that the cultivating cells of the bacteria strains VasShik kiyshk V-1 and VasShik tsdtsspit VM -1 and additionally A / o1oBac1sg antigens A-2, Rkeybotopak ribba PP-1, 81gcr1otuska abbaShk υΖΟΙΤ-1, a sorbent is prepared by mixing vermicompost with carboxymethyl cellulose in a mass ratio of (3-5): (0.5-1.5) mix accordingly cells of these bacterial strains and the remains of a cultured fluid containing metabolites, with - 7 021467 приготовленным сорбентом при следующем соотношении компонентов на 1 г удобрения: клетки указанных штаммов бактерий - 0,06-0,2, указанный сорбент - 0,2-0,3, остатки культуральной жидкости, содержащей метаболиты, - до 1 г, и гранулируют.- 7 021467 prepared sorbent in the following ratio of components per 1 g of fertilizer: cells of these bacterial strains - 0.06-0.2, the specified sorbent - 0.2-0.3, the rest of the culture fluid containing metabolites, up to 1 g, and granulate.
EA201100400A 2011-01-27 2011-03-23 Bacterial fertilizer and method of its preparing EA021467B1 (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD1187Z (en) * 2017-02-08 2018-04-30 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for presowing treatment of tomato seeds
RU2654864C2 (en) * 2016-10-26 2018-05-23 Владимир Михайлович Гавриш Method for obtaining liquid organic fertiliser from waste of organic origin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2157798C2 (en) * 1999-01-21 2000-10-20 Виноградов Евгений Яковлевич Bacterial fertilizer and method of its preparing

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2157798C2 (en) * 1999-01-21 2000-10-20 Виноградов Евгений Яковлевич Bacterial fertilizer and method of its preparing

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DAS B.K. et al. Production of antibacterials from the freshwater alga Euglena viridis (Ehren) World Journal of Microbiology & Biotechnology, 2005, v. 21, No. 1, pp. 45-50, реферат *
IAP20080367 *
MASAAKI MORIKAWA et al. Biofilm formation by a Bacillus subtilis strain that produces gamma-polyglutamate. Microbiology, 2006, 152, pp. 2801-2807, реферат *
U Z -C-2788 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2654864C2 (en) * 2016-10-26 2018-05-23 Владимир Михайлович Гавриш Method for obtaining liquid organic fertiliser from waste of organic origin
MD1187Z (en) * 2017-02-08 2018-04-30 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for presowing treatment of tomato seeds

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