CN109534934A - It is a kind of using discarded jujube tree branch as the preparation method of the efficient growth-promoting bacterial manure of nutrient matrix - Google Patents
It is a kind of using discarded jujube tree branch as the preparation method of the efficient growth-promoting bacterial manure of nutrient matrix Download PDFInfo
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- CN109534934A CN109534934A CN201910073963.7A CN201910073963A CN109534934A CN 109534934 A CN109534934 A CN 109534934A CN 201910073963 A CN201910073963 A CN 201910073963A CN 109534934 A CN109534934 A CN 109534934A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Soil Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a kind of using discarded jujube tree branch as the preparation method of the efficient growth-promoting bacterial manure of matrix, include the following steps: to be seeded in fluid nutrient medium respectively after activating from the Promoting bacteria MX26 bacterial strain, KDZ12 bacterial strain, LZJ3 bacterial strain, the LZJ12 bacterial strain that isolate and purify in rhizosphere soil, at 28 DEG C, 150 r/min shaking table shake culture 12h, obtain I grade of kind liquid, 28 DEG C are forwarded in bacterium minimal medium by 4% I grade of kind liquid of measurement, 150 r/min expand culture 15h, by adjusting bacterium solution OD after culture600, make bacterium amount up to 1.5 × 108 Cfu/mL or so obtains II grade of kind liquid；By each II grade of kind liquid, 1:1:1:1 mixes to obtain flora by volume；It is inoculated with the flora by 15% inoculum concentration, makes fermentation medium by nutrient source of the jujube tree branch of crushing, inoculation flora is placed on the static fermented and cultured 5-6d of 28 DEG C of constant temperature, obtains microbial-bacterial fertilizer.
Description
Technical field
The present invention relates to bacterial manure production fields, and in particular to a kind of using discarded jujube tree branch as the efficient growth-promoting of nutrient matrix
The preparation method of bacterial manure.
Background technique
At present can be applied to saline-alkali soil microbial bacterial agent it is less, reason one be saline-alkali soil environment particularity,
Certain acid-producing bacterias are caused to be difficult to grow wherein, another is that saline-alkali soil belongs to extreme environment, and current separation method does not have also
Enough effective strains can be isolated by having.And a small number of microbial inoculums found out are due to difference existing for saline-alkali soil itself,
It can only be applied in minimum range.
Summary of the invention
To solve the above problems, the present invention provides a kind of using discarded jujube tree branch as the efficient growth-promoting bacterial manure of nutrient matrix
Preparation method.
To achieve the above object, the technical scheme adopted by the invention is as follows:
It is a kind of using discarded jujube tree branch as the preparation method of the efficient growth-promoting bacterial manure of nutrient matrix, include the following steps:
S1, Promoting bacteria MX26 bacterial strain, KDZ12 bacterial strain, LZJ3 bacterial strain, the LZJ12 bacterial strain that will be isolated and purified from rhizosphere soil
(MX26 and LZJ12 bacterial strain is bacillus subtilis, and KDZ12 bacterial strain is bacillus
Cereus, LZJ3 bacterial strain are seeded in fluid nutrient medium respectively after activating for pseudomonad Pseudomonas syringae),
At 28 DEG C, 150 r/min shaking table shake culture 12h obtain I grade of kind liquid, are forwarded to bacterium by 4% I grade of kind liquid of measurement and cultivate substantially
Expand culture in base, after 28 DEG C, 150 r/min shaking table shake culture 15h, by adjusting bacterium solution OD600, make bacterium amount up to 1.5
×108 Cfu/mL obtains II grade of kind liquid；By each II grade of kind liquid, 1:1:1:1 is mixed up to flora by volume；
S2, fermentation medium is made by nutrient source of the jujube tree branch of crushing, is inoculated with the flora by 15% inoculum concentration, is placed in
28 DEG C of constant temperature static gas wave refrigerator 5-6d are to get microbial-bacterial fertilizer.
Further, the material-water ratio 1:1.5 of the fermentation medium, material by following mass percent raw material preparation and
At:
Wheat bran 5%；MgSO4·7H2O 0.05%；K2HPO4·3H2O 0.3%；CaCl2 0.01%；(NH4)2SO4 0.1%；NaCI
0.08%；Jujube tree branch powder 94.46%.
Further, the material-water ratio 1:1.5 of the fermentation medium, material by following mass percent raw material preparation and
At:
Wheat bran 10%；MgSO4·7H2O 0.1%；K2HPO4·3H2O 0.5%；CaCl2 0.008%；(NH4)2SO4 0.5%；NaCI
0.065%；Jujube tree branch powder 88.832%.
Further, the pH of the fermentation medium is 7.2.
Further, primary at interval of 12h button material in the step S2, make flora homoepitaxial.
The invention has the following advantages:
Beneficial microbe in microbial manure can generate a large amount of organic acids during its vital movement, and constantly release is unearthed
Slow effect state nitrogen, phosphorus, potassium in earth, are effectively improved the physicochemical property and soil texture of salt affected soil, increase soil fertility, to utilization
Revegetation improvement salt-soda soil plays a positive role, and microbial manure is at low cost, without secondary pollution.Therefore benefit is studied
Have great importance with microbial manure modified utilization salt-soda soil.But according to ecological principle, particular growth environment, specific kind
The plant rhizosphere of class is survived specific PGPR bacterial strain, so separation, screening PGPR bacterial strain are sampled from former habitat, it can not only
Enough meet the host specificity of different plants, often can also succeed.Carry out the development and strain exploitation of microbial manure
Work, this is the effective measures opening up the source of manure and preserving the ecological environment, and has weight to the reparation and improvement that promote salt-soda soil
The impetus wanted.The microbial-bacterial fertilizer of this method preparation supplies the microbial manure for alleviating Ningxia, China salinization soil
Problem improves the improved effect of salt-tolerant plant, and the process of alkaline land improving is accelerated to have positive impetus.
Detailed description of the invention
Fig. 1 is the additive amount of wheat bran in culture medium, and compound bacterium number changes with time in culture medium when being 5%.
Fig. 2 is the additive amount of wheat bran in culture medium, and compound bacterium number changes with time in culture medium when being 10%.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection scope.
Embodiment
1. bacterium source
Strain isolation is from salt-tolerant plant rhizosphere soil.
The screening of 2 growth-promoting characteristic measurements and efficient Promoting bacteria
The bacterial strain that will be isolated and purified, qualitative and quantitative screening have the PGPR of growth-promoting ability.
Table 1: the measurement of single bacterial strain growth-promoting ability
Note: making quantitative target with A/Ar, and ratio is smaller, reflects that the yield of siderophore is bigger.Referring generally to standard are as follows: A/Ar:0-
0.2 + + + + + ；0.2-0.4 + + + +；0.4-0.6 + + +；0.6-0.8 ++；0.8-1.0 +.
3 efficient plant growth-promoting rhizobacteria Antagonistic reaction experiments
Antagonistic experiment is carried out using the filter paper enzyme different strains high to the growth-promoting of selection activity, obtains 11 plants of not antagonisms each other
Bacterial strain, carry out later period flora building.
Table 2: antagonistic effect result
| MX31 | MX32 | GQ2 | GQ4 | GQ11 | GQ13 | KDZ12 | JJC11 | LZJ3 | LZJ12 | |
| MX26 | ? | ? | + | ? | ? | + | ? | ? | ? | ? |
| MX31 | ? | ++ | ? | ? | + | + | ++ | ? | + | |
| MX32 | ? | ? | ? | + | ? | ? | ? | |||
| GQ2 | ? | ? | ? | ? | ? | ? | ? | |||
| GQ4 | ? | ? | + | ? | ? | ? | ||||
| GQ11 | ? | + | ? | + | ++ | |||||
| GQ13 | ? | + | ? | + | ||||||
| KDZ12 | ? | ? | ? | |||||||
| JJC11 | ? | ? | ||||||||
| LZJ3 | + |
Note: the degree of "+" expression antagonism；"-" indicates not antagonism.
4 efficient PGPR community constructions
It is tested by Antagonistic reaction and the measurement of growth-promoting ability, filters out that growth-promoting ability is strong and the not bacterial strain of antagonism, carried out high
PGPR community construction is imitated, every 4 plants of bacterial strains are one group, totally 9 groups, are shown in Table 4, then quantitative determine the growth-promoting performance of flora, choose most
No. 8 good combinations (MX26+KDZ12+LZJ3+LZJ12) carry out jujube tree branch fermenting experiment.
Table 3: 8 various growth-promoting ability measurement results of combination
Table 4: the growth-promoting ability measurement of composite flora
5 jujube tree branch fermenting experiments
By 4 plants of bacterium plate streaking activated spawns of preservation, oese is seeded in fluid nutrient medium, and at 28 DEG C, 150 r/min are shaken
Bed shake culture 12h is I grade of kind liquid, is forwarded in bacterium minimal medium by 4% I grade of kind liquid of measurement and expands culture 15h, is
II grade of kind liquid, by adjusting bacterium solution OD after culture600, determine bacterium amount up to 1.5 × 108 Each II grade of kind liquid is pressed body by cfu/mL or so
Product is mixed than 1:1:1:1 up to flora.
Using the jujube tree branch of crushing as nutrient source, solid fermentation culture medium is prepared by formula shown in table 5, the basic solid
The flora of building is inoculated with by 15% inoculum concentration, is placed in 28 DEG C of static hairs of constant temperature by pH nature, material-water ratio 1:1.5 in fermentation medium
Ferment culture 8d, it is primary at interval of 12h button material, make its homoepitaxial, is sent out using the method for plate culture count statistics compound bacteria in solid
Growing state in ferment, each gradient set 3 repetitions, wherein plate count culture medium use LB culture medium, the result is shown in Figure 1 and
Fig. 2.
Table 5: jujube tree branch fermentative medium formula
| Number | Wheat bran | MgSO4·7H2O | K2HPO4·3H2O | CaCl2 | (NH4)2SO4 | NaCI | Jujube tree branch |
| 1 | 5% | 0.05% | 0.3% | 0.01% | 0.1% | 0.08% | 94.46% |
| 2 | 10% | 0.1% | 0.5% | 0.008% | 0.5% | 0.065 | 88.832% |
As Figure 1-Figure 2, the composite flora can be well that nutrition industry carries out growth and breeding, fermentation using jujube tree branch
5-6 days bacterium numbers of culture reach highest.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned
Particular implementation, those skilled in the art can make a variety of changes or modify within the scope of the claims, this not shadow
Ring substantive content of the invention.In the absence of conflict, the feature in embodiments herein and embodiment can any phase
Mutually combination.
Claims (5)
1. kind using discarded jujube tree branch as the preparation method of the efficient growth-promoting bacterial manure of nutrient matrix, it is characterised in that: including as follows
Step:
S1, Promoting bacteria MX26 bacterial strain, KDZ12 bacterial strain, LZJ3 bacterial strain, the LZJ12 bacterial strain that will be isolated and purified from rhizosphere soil
It is seeded in fluid nutrient medium respectively after activation, at 28 DEG C, 150 r/min shaking table shake culture 12h obtain I grade of kind liquid, by 4%
It measures I grade of kind liquid and is forwarded in bacterium minimal medium and expand culture, after 28 DEG C, 150 r/min shaking table shake culture 15h,
By adjusting bacterium solution OD600, make bacterium amount up to 1.5 × 108 Cfu/mL obtains II grade of kind liquid；By each II grade of kind liquid 1:1:1 by volume:
1 mixes up to flora；
S2, it is inoculated with above-mentioned flora by 15% inoculum concentration, makes fermentation medium by nutrient source of the jujube tree branch of crushing, is placed in
28 DEG C of constant temperature static gas wave refrigerator 5-6d are to get microbial-bacterial fertilizer.
2. it is described in claim 1 a kind of using discarded jujube tree branch as the preparation method of the efficient growth-promoting bacterial manure of nutrient matrix,
Be characterized in that: the material-water ratio 1:1.5 of the fermentation medium, material are prepared by the raw material of following mass percent:
Wheat bran 5%；MgSO4·7H2O 0.05%；K2HPO4·3H2O 0.3%；CaCl2 0.01%；(NH4)2SO4 0.1%；NaCI
0.08%；Jujube tree branch powder 94.46%.
3. it is described in claim 1 a kind of using discarded jujube tree branch as the preparation method of the efficient growth-promoting bacterial manure of nutrient matrix,
Be characterized in that: the material-water ratio 1:1.5 of the fermentation medium, material are prepared by the raw material of following mass percent:
Wheat bran 10%；MgSO4·7H2O 0.1%；K2HPO4·3H2O 0.5%；CaCl2 0.008%；(NH4)2SO4 0.5%；NaCI
0.065%；Jujube tree branch powder 88.832%.
4. it is described in claim 1 a kind of using discarded jujube tree branch as the preparation method of the efficient growth-promoting bacterial manure of nutrient matrix,
Be characterized in that: the pH of the fermentation medium is 7.2.
5. it is described in claim 1 a kind of using discarded jujube tree branch as the preparation method of the efficient growth-promoting bacterial manure of nutrient matrix,
It is characterized in that: it is primary at interval of 12h button material in the step S2, make flora homoepitaxial.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111606742A (en) * | 2020-05-30 | 2020-09-01 | 博兴天竹菌剂生物科技有限公司 | Special biological source bacterial fertilizer system for winter jujubes and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111606742A (en) * | 2020-05-30 | 2020-09-01 | 博兴天竹菌剂生物科技有限公司 | Special biological source bacterial fertilizer system for winter jujubes and use method thereof |
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