EA015170B1 - High viscosity diutan gums and methods of producing - Google Patents

Abstract

The present invention describes the production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided.; Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.

Description

The present invention describes the preparation of a diutane polysaccharide having an increased viscosity compared to a polysaccharide with the same type of repeating units described in the prior art. The improved properties polysaccharide diutan is obtained using a newly generated strain derived from Zridossoiac 5p. ATTS 53159, containing a multicopy plasmid with a wide range of hosts, into which the genes responsible for the biosynthesis of diutan polysaccharide have been cloned. The plasmid shows the ability to copy the genes responsible for the synthesis of this polysaccharide within the host strain 8p1ipdotop5. Due to this, a method is provided not only to increase the production of the target diutane polysaccharide, but also to obtain the diutane polysaccharide with improved physical properties (with the aforementioned increased viscosity). It has been proven that such a polysaccharide diutane is particularly suitable for use as a possible viscosity modifier in the oil industry and in the production of cement materials. The present invention also encompasses methods for the production of the aforementioned improved diutan polysaccharide, as well as the new cloned genes necessary to obtain improved diutan by these methods. In addition to this, the invention also encompasses a new genetically engineered (recombinant) strain of ZpIDidosoiac, including the desired DNA sequence.

State of the art

Polysaccharides, or gums, are mainly used to increase the viscosity (thickening) or gel formation in aqueous solutions and are often classified into two groups - thickeners and gelling agents. Typical thickeners include starches, xanthan gum, diutan gum, velan gum, guar gum, carboxymethyl cellulose, alginate, methyl cellulose, karaya gum and tragacanth gum. Known gelling agents include gelatin, gellan gum, starch, alginate, pectin, carrageenan, agar, and methyl cellulose.

Some polysaccharides, or, as they are more commonly called, bio-gums, such as xanthan, gellan, velan and diutan, have been obtained from microorganisms by fermentation for many years. These bio-gums exhibit varying characteristics, such as the ability to change viscosity, which allows them to be used in many different areas, including as gelling agents (thickeners) in food products, such as jelly sweets, jams and jellies, jelly-like desserts, icing and dairy products, as well as components of microbiological media. In addition, thickeners are used in numerous cases to modify the viscosity of the target fluids at the final stage. Of particular interest is the ability of these gums to change the viscosity of underground and / or underwater oil fluids, which facilitates their collection, although there are many other possible areas of their final application (including the production of cement as one example). Various bio-gums were obtained from different bacterial sources: for example, xanthan gum was obtained from Hap11utopa 5 satreyke, gellan gum from 8pYpdotopa 5 e1bea, velan gum - from 8pYpdotopa 5p. ATCC 31555 and diutan gum (8-657) - from 8pYpdotop § 5p. ATCC 53159. In the past, genetic modifications of such strains have been undertaken so that a significant modification of the gum materials obtained by the aforementioned fermentation methods is achieved in a more efficient way. The specified modification allowed to achieve such changes as the removal of acyl groups in order to obtain various gum materials with different physical properties. In most cases, these genetic modifications aimed either to ultimately change the composition of the gum obtained by changing the expression of genes in the host organism, or to increase the yield of the target bio-gum by inserting a plasmid capable of amplifying the genes only (see US Pat. Nos. 5,854,034, 5,985,623 and 6284516 (Po11osk e! A1.) And US patent No. 6709845 (only Po11osk).

Diutan gum (also known as heteropolysaccharide 8-657) is obtained by fermentation of strain 8p1shchotop5 5p. ATCC 53159; in aqueous solutions, it has a thickening, suspending and stabilizing ability. In most cases, diutan shows a hexameric structure of repeating units consisting of four sugars in a framework (glucose-glucuronic acid-glucose-rhamnose) and a side chain of two rhamnose residues attached to one of the glucose residues. For details on the structure of diutane gum, see the article S1yu ub1shgu T.A., Ybbetd V., bshddshB! and. and Vaib 1., Satyubbae Vekeatsy, 164 (1987), 117-122. Όί1ΐζ e! a1. (Satyubbae Vekeatsy, 331 (2001), 265-270) showed that diutane has two acetyl substituents per one repeating unit. Both of these links are fully included in the list of links to this application. Details of the preparation of diutane gum can be found in US Pat. No. 5,175,278, which is incorporated in its entirety into the list of references to this application. Diutan can be obtained from strain 8p1ipdotop5 using a standard fermentation technique using, for example, carbohydrate sources (non-limiting examples of which are glucose, maltose, etc.), a nitrogen source, and additional salts.

The physical characteristics imparted by such diutane bio-gum in its wild form are in demand in certain areas of industry, in particular, in the framework of its ability to modify viscosity and / or water-holding characteristics. Unfortunately, it has been proven that achieving effective

- 1 015170 in terms of cost of producing diutane is extremely difficult. In addition, the problems associated with these costs hinder the widespread use of diutan at the present time, since the degree of viscosity exhibited by this bio-gum is insufficient to crowd out other, less expensive but effective bio-gums (such as xanthan gum as one from examples). Essentially, there is an urgent need to provide an efficient method for the production of diutane with the lowest possible cost and / or to provide a method for the production of diutane-type bio-gum, showing a significant improvement in its physical properties. To date, the only mention of the production of any kind of related sphingans (without specific reference to diutan) is to increase their yield (see the aforementioned patents Po11osk s1 a1.). There are currently no publications on the discussion or on any clearly formulated proposal on a possible way to provide a method for producing an improved diutane gum with a higher molecular weight, which would show an improvement in viscosity in measurements.

SUMMARY OF THE INVENTION

The inventors were able to clearly demonstrate that the amplification of certain newly isolated DNA sequences for the biosynthesis of diutan in the host organism ЗрЫидошоевак not only provides an increase in the production of diutan from it, but also allows to obtain diutan gum, which has a high viscosity. This new DNA sequence (which is introduced into the host organism by a well-known method, for example (but their list is not limited to the one mentioned here) using a plasmid) provides the required results that were constantly expected from diutan biosynthesis methods. One of the advantages of amplification of these genes in a plasmid is the relatively simple nature of introducing an isolated DNA sequence into diutan synthesis processes. Another advantage is the ability to impart increased viscosity to the resulting diutane gum, while at the same time potentially increasing, if necessary, the fermentation efficiency.

Thus, the present invention includes diutane gum with improved viscosity, measured in various ways. Among them: (ί) an intrinsic (or intrinsic, i.e. organically inherent) viscosity of more than 150, preferably more than 155, more preferably higher than 160 dl / g; (ίί) a viscosity in sea water at 3 rpm./min, comprising more than 35, preferably more than 37, more preferably more than 40 and most preferably more than 42 indications of the scale of the device; (ίίί) a viscosity in sea water at 0.3 rpm./min, greater than 35,000, preferably higher than 39,000, more preferably higher than 40,000 and most preferably higher than 41,000 centipoise (cP); (ίν) viscosity in PEC (polyethylene glycol) at a low shear rate of above 3500, preferably above 3700, more preferably above 3900 and most preferably above 4000 cP. The invention also includes a method for producing diutan gum, defined by any of the above terms, by introducing a specific cluster of genes into the host organism and carrying out the fermentation of the specified organism to obtain the desired diutan gum. In addition, the present invention also includes specific DNA sequences and any vector (e.g., plasmid) providing multiple copies of genes or increased gene expression using a stronger promoter and the like. In addition to this, the invention also includes a genetically modified strain of pIDidossoiac containing multiple copies of genes that control the biosynthesis of diutan and are determined by these unique isolated DNA sequences.

This unique isolated DNA sequence has been found to require at least one enzyme that catalyzes the biosynthesis of diutan, and this enzyme is Er ^ C polymerase. In another possible embodiment, the enzyme catalyzing diutan biosynthesis comprises Er ^ C polymerase and glucose-1-phosphate thymidyl transferase; άΤΏΡ-6-deoxy-O-glucose-3-5-epimerase; bTPR-O-glucose-4,6-dehydratase and άΤΏΡ-6-deoxy-E-mannose dehydrogenase. In yet another possible embodiment, the enzyme catalyzing the biosynthesis of diutan includes Er ^ C polymerase and ramnosyl transferase IV; beta-1,4-glucuronosyltransferase II; glucosyl isoprenyl phosphate transferase I and glucosyl transferase III. In a further possible embodiment, the enzyme that catalyzes the biosynthesis of diutan comprises brgC polymerase and polysaccharide transporting proteins br ^ E. br§C and br§E. In yet another embodiment, the diutan biosynthesis enzyme comprises rhamnosyl transferase IV; beta-1,4-glucuronosyltransferase II; glucosyl isoprenyl phosphate transferase I; glucosyl transferase III; glucose-1-phosphate thymidyl transferase; bTPR-6-deoxy-O-glucose-3-5-epimerase; btp-O-glucose-4,6-dehydratase and btp-6-deoxy-b-mannose dehydrogenase. In most cases, the enzyme that catalyzes the biosynthesis of diutan, according to the method of the present invention and within the product of the invention can be selected from the group consisting of polymerase; lyases; ramnosyl transferase IV; beta-1,4-glucuronosyltransferase II; glucosyl transferase III; polysaccharide transporting protein; secreting protein; glucosyl isoprenyl phosphate transferase I; glucose-1-phosphattimidyl transferase; bTPR-6-deoxy-P-glucose-3-5-epimerase; bTPR-E-glucose-4,6-dehydratase; btp-6-deoxy-b-mannose dehydrogenase and combinations of the above. Also present

The invention includes an isolated nucleic acid molecule (in addition to DNA, which may be present on the target chromosome), which encodes at least one enzyme that catalyzes the biosynthesis of diutan, as shown in 8EO ΙΌ N0: 5, 7, 9, 11 , 13, 15, 17, 19, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41 and 43, or an enzyme that is at least 95% identical to 8E0 ΙΌ N0: 5, 7, 9, 11, 13, 15, 17, 19, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41 and 43.

The method of the invention (as well as the products obtained by it) relates to sphingan gums, in particular diutan species, including (but not limited to only those mentioned here) 888, 860 and 8657.

As noted above, the present invention is the culmination of the development and implementation of a method in which specific DNA sequences, when introduced in multiple copies into specific strains of 8pYdotoia8, can provide biosynthesis in an increased volume of the highly viscous diutan polysaccharide. Genetically engineered (recombinant) bacteria containing genes responsible for increased production of diutan produce much higher amounts of diutan polysaccharide compared to non-genetically engineered bacteria and provide its aforementioned high final viscosity.

DNA sequences that are introduced into the host organism (in any well-known form, such as a plasmid, but the list is not limited to the named form) for increased production and increased viscosity (due, as expected, without reference to any specific scientific theory molecular weight increase) in accordance with the present invention, can be isolated, extracted and cloned by methods known from the prior art. After this, the DNA is introduced into bacteria of the 8pYpdotopa genus § in multiple copies (using a plasmid or other known method) or by increasing the expression of genes using a suitable, for example, stronger promoter. After insertion into the target bacterium, the production of diutan can be determined by the fermentation of genetically engineered bacteria and the comparison of the yield in terms of the quantity produced and the quality produced. Both the increase in production and the increase in viscosity can be determined by a comparative evaluation of the production of diutan by the method of the invention and the production of diutan using a wild strain (ATCC 53159) capable of producing diutan.

Brief Description of the Drawings

In FIG. 1 schematically shows isolated genes that control the biosynthesis of diutan gum; putative or known genes are shown; fragments embedded in various plasmids are also shown;

in FIG. 2 graphically presents the results of measurements of internal (intrinsic) viscosity, showing its increase when using diutane bio-gum materials according to the invention.

DETAILED DESCRIPTION OF THE INVENTION

The following terms are used in the description of the present invention, the meaning of which is disclosed below.

The term 8pYpdotopa5, used in the description, refers to strains of gram-negative bacteria of the genus 8pYpdotopa§.

The term producer or increased production used in the description describes genetically engineered bacteria containing multiple copies of DNA sequences isolated from a strain of such bacteria that produces a significantly higher (at least about 5 wt.% In terms of weight) amount of polysaccharide diutane compared with wild-type bacteria of the same strain.

The term isolated, isolated is used to describe DNA that has been isolated from a microorganism and subjected to at least some degree of purification, for example, one or more purification steps, and which can be digested with restriction enzymes, cloned in multiple copies, or inserted into plasmid vectors or injected into bacteria in some other way.

The term sequence is used to describe a specific DNA fragment that is identified by a nucleotide sequence. The term “built-in” is used to describe the process and transfer efficiency of DNA fragments isolated from chromosomal DNA capable of producing diutan of strain 8рЫпдотота§, to the strain 8рЫпдотопа§ (using a plasmid, but the list is not limited to this example only). This isolated DNA can be introduced first (and in this case the list is not limited only by the indicated possibility) into the desired plasmid (in this case pLEC.3) by a method well known in the art and then transferred, for example, by conjugation or mobilization into a recipient bacterium §. After 8pYpdotopa§ has been introduced into the recipient bacterium, the plasmid containing the relevant DNA sequence will be replicated many times in the recipient cell, providing several (at least two, usually four to ten) copies of the DNA fragment necessary for increased production of high viscosity diutan polysaccharide (due again, as expected, to an increase in molecular weight). The use of conjugation or mobilization to transfer plasmid vectors to the recipient bacterium is quite effective in most cases. You can also apply electroporation

- 3 015170 or chemical transformation of competent cells of purified DNA. Other vectors and bacteriophages can be used to transfer DNA to the host cell. There is no need to maintain DNA fragments on plasmids (or other well-known transfer vectors) in a diutane-producing 8p1npotopack recipient. It is common practice to introduce additional copies of the DNA fragment into the bacterial chromosome so that the mechanism of fragment replication by each generation is identical to the mechanism of bacterial DNA replication. Alternatively, enhanced gene expression can be achieved through the use of stronger promoters.

The term gene amplification refers either to an increase in gene copies, for example, cloning of target genes into a multicopy plasmid (e.g., from 4 to 10 copies) or insertion of multiple copies (e.g., from 4 to 10) genes in the bacterial genome, or, alternatively, to an amplified gene expression modification of the promoter to enhance gene expression. Both of these methods, like others, can lead to increased production of encoded proteins.

The term biosynthesis is used to describe the biological production or synthesis of diutan by the bacteria Zrpdotopak. The polysaccharide diutane is synthesized from individual carbohydrate units in series in several stages, controlled by a large number of bacterial enzymes.

The relevant DNA sequence, which is introduced into the recipient bacterium in any selected form (preferably again, but not necessarily, in the form of a plasmid) encodes genetic information that is known to be useful or essential for the biosynthesis of an increased amount of diutan polysaccharide with an increased molecular weight. Nevertheless, it is additionally suggested (without reference to a specific scientific theory) that the DNA sequence according to the invention (such as in plasmid p88) induces not only an increase in the production of diutan, but also an increase in the number of repeating units polymerizing into individual polymers of diutan itself. As a result of this, it is assumed that this increase in the number of repeating units leads to an increase in the final viscosity, surprisingly provided by diutan gum. It has been hypothesized that the increase in molecular weight is due to a measurable increase in true viscosity, which is associated with a molecular mass that has the strength of the law of dependence. For a linear polymer (which is diutan gum), the true viscosity is known to be proportional to essentially the molecular weight.

The selection of relevant DNA sequences that form the basis of the method of the present invention and which generate an increased viscosity of diutan polysaccharide is carried out by standard methods. Therefore, such sequences can be generated from the dutane-producing strain 8pYpdotopac cultured under standard conditions. DNA extraction can then be carried out, for example, by initial centrifugation and resuspension of bacterial cells and subsequent elution of the DNA through purification columns. At the end of the purification, the isolated DNA can be digested with restriction endonucleases and cloned into the desired plasmid or other vector, followed by introduction into the recipient strain. You can use other methods known from the prior art and not limiting the entire list of methods.

DNA cloning in the present invention is based on generally accepted techniques and methods that have become standard in the art. It should be noted that any number of methods can be used for cloning DNA fragments in accordance with the present invention and that the present invention is not limited, for example, to the use of plasmid cloning vectors only. For example, DNA fragments can be cloned by insertion into a bacteriophage-based vector.

The cloned DNA sequences can then be introduced into the SpDotopotak strain using a plasmid or other vector. For the production of diutan by fermentation, then a genetically modified strain of 8pYpdotopac can be used. A generally suitable fermentation medium is an aqueous medium that typically contains a carbon source, such as, for example, carbohydrates including glucose, lactose, maltose or maltodextrins; a nitrogen source, such as, for example, inorganic ammonium, inorganic nitrate, organic amino acids or protein materials, such as hydrolyzed yeast, soy flour or casein, barda extract or liquid corn extract, and inorganic salts. The production of diutans in accordance with the present invention can be supported by a wide range of diverse fermentation culture media.

Carbohydrates can be included in the fermentation broth in varying amounts, but usually in an amount of about 1 to 10 wt.% (Preferably 2 to 8%) of the fermentation medium. Carbohydrates may be added before fermentation or, alternatively, during the fermentation process. The amount of nitrogen can range from about 0.01 to 0.4 wt.% Aqueous nutrient medium. Only one carbon source or nitrogen source may be used, as well as mixtures of these sources. Of inorganic salts that can be used in the process of fermentation of bacteria 8p1npdotopak. can be called salts that contain ions of sodium, potassium, ammonium, nitrate, calcium, phosphate, sulfate, chloride, carbonate and similar ions. Advantageously, trace amounts of metals such as magnesium, manganese, cobalt, iron, zinc, copper, molybdenum, iodide and borate may also be included.

Fermentation can be carried out at temperatures from about 25 to 40 ° C, more preferred

- 4 015170 is a temperature range from about 27 to 35 ° C. The inoculum can be prepared by standard methods in volumetric proportions, including cultures in shaken bottles and small-scale deep fermentation under stirring. The culture medium for the preparation of the inoculum can be a production medium or any of a number of standard nutrient media well known in the art, for example, Lst broth or S medium. To obtain the required volume of inoculum, more than one inoculum stage can be carried out. Typical inoculum volumes range from about 0.5 to 10% of the total final fermentation volume.

The fermenter may be equipped with a stirrer to mix the contents. The fermenter can also be equipped with automatic pH control and foaming devices. The production medium can be added to the fermenter and sterilized therein by appropriate heat treatment. Alternatively, the carbohydrate source or carbon source may be sterilized individually prior to addition. Pre-germinated seed can be added to the chilled medium (in most cases, preferably at a fermentation temperature of about 27 to 35 ° C), and the mixed culture can be fermented for about 48 to 110 hours until a highly viscous broth is formed. The polysaccharide diutane can be recovered from the broth by the standard precipitation method with alcohol, in most cases isopropanol.

Information confirming the possibility of carrying out the invention

The following examples are provided to illustrate the present invention. The description of the examples should in no way be construed as limiting the scope of the present invention.

DNA sequence / plasmid creation

Prior to the initial isolation and determination of the appropriate sequence for the above described results of the invention, the ATCC 53159 gene library of the organism was created: from 8p1idotoiac cr. ATCC 53159 was isolated chromosomal DNA and partially treated with 8Ai3A1 restriction endonuclease. DNA fragments from 15 to 50 kB long (thousand pairs of nucleotides) were purified from an agarose gel and ligated into cleaved BatH1, a cloning vector cosmid pAEK3 (according to gase 1 кеί кеке ке ий куп куп куп куп пас ρ С С С С куп 1987 1987 1987 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9. sh. ChaiNotoiak satrekRtk, Вас. Vas1etiuodu, 1987, 169: 2854-61). The ligation reactions were carried out under conditions of packaging in λ phage particles (using C1darask III Co1y extract as a package from 81ta1adeie, La yoPa, CA) and were transformed into Ltata E. coli E. coli cells (Lye Tesuioideus, Bocxe, . A pool of approximately 10,000 tetracycline resistant colonies was selected to form a gene library. Separate sequences were subsequently isolated from this library. The work carried out in this example included the isolation of specific genes for the biosynthesis of polysaccharides from the organism of 8pYidothioiac ATCC 53159.

These genes for polysaccharide biosynthesis in typical cases are identified by complementation of mutants with impaired ability to synthesize polysaccharides, in particular mutants blocked at the first stage, which lost their specificity for glycosyl transferase I. Since initially there were no defective ones, i.e. with impaired specificity for transferase I, ATCC 53159 mutants, complementation of defective ones, i.e. with impaired specificity for transferase I, mutants 8py1idotoiak e1oyea and KhaiNotoiak satreisk used to identify genes responsible for the biosynthesis of diutan polysaccharide. The plasmid pAEK.3 can be transferred from the host E. coli to other gram-negative bacteria by conjugation of the three parent groups using an auxiliary (helper) plasmid, which gives the ccp transfer function (according to 0111a e1 a1. -ieda1she as1epa: soikyisyoi οί deie aik οί RYhosht tey1oy, Proc Acai 8sk Wu (1980) 77:... 7347-51). Plasmids of type VK2 have an estimated number of copies in E. coli from five to seven per chromosome (B1ditkk1 e1 a1., 8irrtekky ui Co1E1 terisoyoi rtoretyeku 1yu ShsR-1 p1akt1 VK2 shyuyu pyaktyuyu. 1979), 133: 295-318).

The ATCC 53159 gene library of chromosomal DNA in E. soy was transferred to the nemcoid mutant (CP82) 8. e1oya ATCC 31461 (conjugation of three parent groups), chosen for its resistance to tetracycline and streptomycin. Plasmid pKK2013 was used as helper (in E. soybean strain ίΖ279), which contains replications for a narrow host spectrum, but is suitable for mobilization of rEABKZ. Plasmid rVK2013 was not replicated in strains of 8 rdshdoiak. 8. e1oyea ATCC 31461 produces the gellan polysaccharide. Both the gellan polysaccharide and the diutan polysaccharide contain the same repeating tetrasaccharide unit consisting of 04) -a-E-ramnose- (1 ^ 3) -B-O-glucose- (1 ^ -4) β-glucuronic acid- (1 ^ -4) -in-O-glucose- (10. However, diutane also has a side chain containing two rhamnose molecules attached to one of the glucose residues and is modified by acetyl, while gellan has no side chain of sugars and modified by acetyl and glyceryl The CP82 mutant is defective at the first stage of polysaccharide biosynthesis, i.e., at the stage of glucose-1-phosph transfer from IPR-E-glucose to a bactoprenyl phosphate-lipid carrier with the enzyme glucose cosyltransferase I. Polysaccharides (mucoid) colonies were isolated from plates with tetracycline from the general background of non-mucoid colonies. Clones that restore production of polysaccharides A, presumably 53159, encoding glucosyltransferase I plus about 20 to 25 kb of contiguous DNA, plasmid DNA was isolated from eight mucoid CP82 transconjugates and transferred to the E. coli strain S5α (Lye Thcoconosyl 5) by electroporation. Plasmids were isolated from E. soy so as to obtain an amount of DNA sufficient for double digestion with restriction endonucleases Hsh / EsO! (which are located in the polymer on both sides of the WatH1 site) in order to remove the DNA insert from the vector. The size of the DNA stakes in clones was determined by gel electrophoresis. The terminal sequences of several plasmids were determined by sequencing from primers specific for plasmid sequences flanking the WatH1 region of the vector. The analysis of the sequences was carried out by comparing them with sequences in computer databases using the software package ABAX. Two of these plasmids, p88 and p86, are shown in FIG. 1. Similarly, the ATCC 53159 gene library was transferred to the rifampicin-resistant, non-mucoid, defective mutant X. satrs51p5. having lost its specificity for transferase I (CXC109) (which is described by Natishd! a1., see link above), by conjugation of the three parent groups selected for their resistance to tetracycline and rifampicin. X satrays produces the xanthan polysaccharide, the synthesis of which is also initiated by the transfer of glucose-1-phosphate from iER-Eglucose to the bacteroprenyl phosphate-lipid carrier by the transferase I enzyme (1c1p1 cis a1., 8cc | ish! οί! ys haiShai ro1uzassyapys ίη HaiShotoiaz satrszytz, Вас. You! stuyoogu (1993), 175: 2490-500). Plasmids were purified from mucoid transconjugates, after which the terminal sequences were determined as described above. Two of these plasmids, pX6 and pX4, are shown in FIG. one.

8657 DNA cloned into p88 and pX6 plasmids was completely sequenced by double-stranded sequencing using the method of 8th method at the company Lutk Tssyio1od1c8 Sch., (Noisy, TX). Next, an analysis of these sequences was carried out in order to identify the genes responsible for the biosynthesis of diutan (shown in Fig. 1). The functions of the genes were determined on the basis of their homology with other genes in the databases, in particular with the published genes responsible for the biosynthesis of sphingan 8-88 (such as described in the aforementioned '516 patent, issued by Po11osc c! A1.), Access number in CsiVaik I51197, and Gellan (CsiVaik ΑΥ217008 and ΑΥ220099). Genes were identified (FIG. 1) encoding transferases for four scaffold sugars and four genes responsible for the synthesis of DTER-rhamnose. The polysaccharide secreting genes were identified based on their homology with the genes responsible for the biosynthesis of other polysaccharides. Two genes encode proteins homologous to proteins involved in protein secretion. Two genes are believed to encode polymerase and lyase. The insert in plasmid pX6 contained 17 genes, including the srcB gene encoding transferase I (which initiates the first step in the synthesis of diutan), the genes responsible for secretion, and four genes responsible for the synthesis of DTER-rhamnose, but there were no genes responsible for transferases II, III and IV, and genes presumably responsible for polymerase and lyase. Plasmid p88 contains 20 genes of a cluster of yrs genes, including genes responsible for all transferases of four carcass sugars, four genes responsible for the synthesis of DTER-rhamnose, and genes responsible for polysaccharide secretion, including genes presumably responsible for polymerase and lyase, but it lacked genes with an unknown function - оТ6 and exL. Plasmid p86 contains genes responsible for the secretion and transferase of four sugars, but does not have all the genes responsible for the synthesis of DTER-rhamnose, or the gene responsible for polymerase. Plasmid pX4 contains only a small portion of the yrs site, but includes the gene encoding transferase I, and four genes responsible for the synthesis of DHOR-ramnose, which, according to Po11osk! A1., it is quite enough to achieve increased production of polysaccharide in strains of 8pIDidothioiasis.

Obtaining strains

The four plasmids described above were then introduced into the 8pOidotoiiasis ATCC L0 strain. 53159 by conjugation of three parent groups, as described above, in order to create new recombinant (genetically engineered) strains 8657 (8657 / p88, 8657 / p86, 8567 / pX6 and 8657 / pX4). Then they were fermented, as described above, in order to initiate the production of bio-gum, as noted below. All four plasmids had a beneficial effect on diutane production productivity; however, plasmid p88 surprisingly also provided an extremely strong increase in the viscosity of diutan and an increase in its molecular weight. The DNA sequence of plasmid p88 (26278 Lp3) was also provided (ΌΝΑ 8dississ L0. 1); encoded genes are listed in table. 1 (see below) and in the form of a diagram in FIG. 1. The DNA insert into the p88 plasmid includes the srcC genes through rt1E and part of the srp8 and exL genes.

The following gene table provides basically a list of genes represented by the sequence of the DNA insert into plasmid p88, as shown in FIG. one.

- 6 015170

Table 1

Genes on plasmid insert p88

Start the end Designation Description 2 * 1054 brz8 (partial) homology with ββΐζ 2738 1113 C brzs presumably responsible for polymerase 4895 2898 C brzk presumably responsible for lyase 5093 6031 brz () presumably responsible for rhamnosyl transferase IV 7082 6111 C brz1 unknown genes 7121 8167 brzk responsible for beta-1,4-glucuronosyl transferase II 8164 9030 brze responsible for glucosyl transferase III 10467 9079 C brz1 unknown genes 11076 12374 brzG unknown genes 12389 13306 brzb presumably responsible for polysaccharide transporting protein 13341 14687 brzs presumably responsible for polysaccharide transporting protein Start the end Designation Description 14687 15394 brze presumably responsible for polysaccharide transporting protein 15405 16286 brzM presumably responsible for polysaccharide transporting protein 16270 16968 άρβΝ presumably responsible for polysaccharide transporting protein 18454 17060 C iB presumably responsible for protein secretion 20637 18451 C iV presumably responsible for protein secretion 21229 22641 brzv responsible for glucosyl isoprenyl phosphate transferase I 22757 23635 gt1a glucose-1-phosphate thymidyl transferase 23632 24198 gt1s responsible for sGBHB-b-deoxy-B-glucose-3-5epimerase 24202 25263 gt1v responsible for btbr-b-glucose-4,6-dehydratase 25263 26129 rm! b responsible for β-6-deoxy-β-mannose dehydrogenase 26277 * 26146 C Og17 function (partially) unknown

* The first codon in the reading frame, the start codon is not present.

Getting Dutane

The production of diutane by genetically engineered plasmid-containing strains of 8pYpdotopaz 8657 in comparison with the wild strain 8657 without plasmid was determined in three series of experiments on fermentation in the same liquid nutrient medium in ArrNcop fermenters with a capacity of 20 l with mixing and aeration. In the case of strains containing the plasmid, the antibiotic tetracycline in an amount of 5 mg / L was added during the fermentation to preserve the plasmid. KOH was added as needed to control pH. Two stages of inoculation were carried out with transfers from 1 to 6% of the inoculum. The nutrient medium used for the fermentation contained corn syrup as a source of carbohydrate, a source of digestible nitrogen and salt. Nutrients that can be used for fermentation are well known in the art and include a carbohydrate, for example glucose, sucrose, maltose or maltodextrins; a nitrogen source, for example, a source of inorganic nitrogen, such as ammonium or nitrate; a source of organic nitrogen, such as amino acids, hydrolyzed yeast extract, soy protein or liquid corn extract; and additional salts, including, for example, chloride, phosphate, sulfate, calcium, copper, iron, magnesium, potassium, sodium, or zinc.

A measure of the final production of diutan was the viscosity of the broth and the amount of precipitated fibers. The viscosity of the fermentation broth was measured with a Brookfield viscometer (BgoocieI) at 60 rpm using a # 4 spindle; the measurement results are presented in table. 2. At the end of the fermentation, the well-known glucoamylase enzyme was introduced into the broths to hydrolyze the residual oligosaccharides from the corn extract. Then, from the aliquot of the broth, the accumulated diutan gum was precipitated with two volumes of isopropyl alcohol. The fibers were collected on a filter and dried. In the table. 2 abbreviation Ό ^ Υ means the total yield of precipitated bio-gum in terms of dry weight after hydrolysis of excess oligosaccharides from corn extract.

From the data table. 2 clearly shows that the increased yield of the target material due to the presence of

- 7 015170 plasmids pX4, pX6, p86 or p88, carrying additional copies of the genes responsible for the biosynthesis of diutan. However, in the case of plasmid p88, coupled with an increase in yield in terms of dry weight, an unexpected sharp increase in the viscosity of the broth was observed, which indicates the presence of a certain influence factor on the viscosity in addition to an increase in the amount of diutane produced.

table 2

Fermentation of plasmid-containing strains

υν / γ Strain Series of experiments # one Series of experiments # 2 Experiment Series # 3 Average % increase 8657 33.9 8657 / p88 34.3 32,2 35.9 33.5 8.0% 8657 / pX6 37.1 35,4 33.5 36.1 9.1% 8657 / p86 38,4 37.6 37.6 36.5 12.3% 8657 / pX4 36,4 8.8% Broth viscosity Strain Experiment Series # 1 Series of experiments # 2 Experiment Series # 3 Average % increase 8657 5550 8657 / p88 5150 4950 6850 5217 30.0% 8657 / pX6 6650 6850 5125 6783 7.2% 8657 / p86 5400 6250 6675 5592 28.0% 8657 / pX4 5525 5.9%

It is obvious that an increased yield of the final material was ensured by any of the four plasmids present, while plasmids p88 and p86 provided an unexpectedly sharp increase in the viscosity of the broth, which also indicates a high quality product. Therefore, the quality, i.e., viscosity, of the resulting diutane gum products was further determined.

Rheology of diutan in tests for its use

Samples of the resulting diutane gum were analyzed for their potential intended use in two different areas: in the oil industry as additives for oil recovery and in the production of cement as additives for moisture retention and rapid capping.

The oil industry as a whole depends on what the term viscosity test in seawater (8SH) denotes as an estimate of the acceptable gum productivity in oil recovery. Such a test is mainly an indicator of the effectiveness of gum for increasing the viscosity in water of high salinity (for production from the seabed, for example).

Prediction of the possibility of using the obtained gum as an acceptable viscosity modifier in the field of oil production in most cases is based on the results of a viscosity modification test in a composition similar to sea water. Such a composition of artificial sea water is obtained by mixing 419.53 g of sea salt (A8TM Ό-1141-52) in 9800 g of deionized water. To conduct a viscosity test in seawater, 0.86 g of a gum sample is added to 307.0 g of artificial seawater and mixed at about 11,500 rpm in a universal Gagsh Mi1t1heg mixer (model 9B5, product number N5020) for 35 minutes. After 35 minutes, the solution is cooled to approximately 26 ° C. before viscosity measurements are taken. To obtain the readings of the instrument scale at 3 rpm, the sample is placed on the Tapi sample platform (model 35 A; MOS 34/35 E0.2 torsion spring; plumb (pendulum) B1; rotor Κ1) and the rotation speed is set to 3 rpm, by turning the electric motor to the low speed position and setting the gear selector to the middle position. Then, the scale indication is allowed to stabilize, the shear stress value is read from the scale of the device and recorded as a scale reading (ΌΚ) of 8CHU-3 rpm. To determine the viscosity at 0.3 rpm, a Brookfield viscometer is used (Brookfield viscometer LU ОУ-ΙΙ or ОУ-ΙΙ with a spindle БУ-2С). The spindle rotation speed is set to 0.3 rpm and the spindle is allowed to rotate for at least 6 minutes before reading 8CHU-0.3 rpm and expressing it in centipoises (cP). To determine suitability for the cement industry, a REO B8KU test is carried out (a test for determining viscosity at a low shear rate using polyethylene glycol (PEO) as a dispersant, as indicated below), which provides information on the effectiveness of using a viscosity modifier in this industry. The specified test measures the viscosity of a 0.25% solution of bio-gum in standard tap water (8TC). 8TCH is prepared by adding 10.0 g of No. Cl and 1.47 g of CaCl ₂ -H ₂ O to 10 L of deionized water. To measure the viscosity, 0.75 g of bio-gum is added to 4.5 g of polyethylene glycol 200 (CA8 25322-68-3) in a 400 ml beaker and thoroughly dispersed. Then 299 g of 8T ^ was added to the beaker and stirred for about 4 hours using a low-slope propeller stirrer at 800 ± 20 rpm. At the end of the 4-hour stirring, the beaker was placed in a 25 ° C water bath and the contents of the beaker allowed to settle without stirring for about 30 minutes. After that, the viscosity is measured with a Brookfield bis viscometer, equipped

- 8 015170 with a 2.5+ torsion spring (or a similar device, for example, the model EUE 2.5+), at 3 rpm using the BU1 spindle, which is allowed to rotate for 3 minutes; measured viscosity is expressed in centipoises (cP).

Samples of the resulting diutane were tested in this way; The test results were as follows.

Table 3

Rheology of diutan from plasmid containing strains

Strain 8 \ U7 - 3 rpm. PC (scale reading) 8Ψν-0.3 rpm. cp REO L8KU SP Experiment Series # 1 Series of experiments # 2 Series of experiments # 3 Experiment Series # 1 Series of experiments # 2 Series of experiments #one Series of experiments # 2 Experiment Series # 3 8657 (wild 25 26 22 24,400 28600 2820 3150 2280 strain) 8657 / p88 42 43 47 41500 38800 4720 4980 4920 8657 / pX6 25 29th 26 25,000 29100 2860 3400 3270 8657 / p86 - ___ 22 ... - - ... 2270 8657 / pX4 ___ ... 24.5 ... ... ... ... 2950

8A'U = viscosity in sea water

1.8VU = low shear viscosity

Quite unexpectedly, a certain increase in the viscosity of the diutan gums of the invention produced by certain genetic engineering plasmid-containing strains was found. However, the most surprising was that an increase in viscosity of 8 ^ Y-3 rpm in the case of the strain with plasmid p88 was 80%, while in the case of the strain with plasmid pX6 it was only 9.6% compared to the wild strain. Plasmids p86 and pX4 did not lead to a significant increase in viscosity. Similarly, the test for measuring 8 ^ Y at a lower rotation speed (rpm) revealed, compared with the wild strain, an increase in viscosity of about 51.5% in the case of plasmid p88 versus a little more than 2% in the case of plasmid pX6. And finally, the B8KU test with polyethylene glycol showed that plasmid p88 led to an excessive increase (77%) in the viscosity of diutan gum compared to the gum produced by the wild strain (without plasmid); plasmid pX6 provided an increase in viscosity of less than 16%, plasmid pX4 by 7.2%, and plasmid p86 did not significantly increase the viscosity. And again, these unexpected test results indicate a dramatic improvement in the production of diutan gum using the necessary gene sequence integrated into the p88 plasmid, as one of the methods for introducing such a sequence into the target target capable of producing diutan.

Thus, the method of producing diutan according to the invention with the introduction of p88 showed surprisingly high viscosity measurements in all three tests, in particular compared to wild strains and strains with plasmid pX6. As expected, this new diutan functioned extremely well under typical conditions of the oil and cement industries.

Fundamental explanation for improving diutan rheology

The above examples showed that diutan obtained from strain 8657 / p88 had a significant improvement in rheological parameters. This significant increase in viscosity when measured in seawater and at a low shear rate with PEO cannot be explained only by an increase in productivity, since the pX6 strain also showed similar results (if not higher) in the yield of diutane. In fact, in the example described above and the related table. 2, the yield in terms of dry weight (substance precipitated by alcohol) increased by 8.0% while at the same time a significantly larger increase in rheological parameters in the case of strain 8657 / p88 (from 52 to 80%). A fundamental study was undertaken to identify the reasons for such a significant improvement in rheology in the case of strain 8657 / p88 compared to the wild strain.

Internal (intrinsic) viscosity is a well-known concept in the science of polymers for the deduction of the molecular mass of macromolecules (TapGogy S., 1961, Ryu81ca1 Siet181yu £ Masgoto1esi1e8, .1obn M1eu & 8op8, Hesh Wogk). The internal viscosity is obtained by plotting the curves of the dependence of reduced viscosity (viscosity reduced to concentration) on the solution concentration and extrapolation of the data by linear regression to zero concentration (on the y-axis of the graph). The resulting gums surprisingly had increased internal viscosity, which is obvious from the table below.

Five samples of diutan (two from the wild strain (control 1, control 2) and three from the 8657 / p88 strain (sample 1, sample 2, sample 3)) were evaluated by the results of analyzes for internal viscosity, the content of neutral sugars and organic acids. These samples were purified by precipitation with alcohol, rehydrated, treated with hypochlorite, treated with glucoamylase, treated with lyso

- 9 015170 tsimom and in the end was treated with a protease (in this sequence). Then they were extracted in the ratio of CBM: broth = 4: 1. CBM is an azeotropic mixture of isopropyl alcohol / water, including ~ 82 wt.% Isopropyl alcohol.

The samples were tested for moisture content as follows: basically two aliquots (0.7 g each) of the sample were tested using a halogen moisture meter MeE1eg HB. Then the results of two tests were averaged and the newly obtained results were used to adjust the humidity.

After obtaining the moisture content data, a 0.2% solution of gum in a 0.01 M solution of aC1 was prepared taking into account the adjusted humidity. For these tests, only 200 g of a 0.2% solution was prepared. Gum was weighed on an analytical balance with an accuracy of one ten thousandth and added to water suspended with an accuracy of one thousandth. Samples were mixed for two hours using a 2.5 inch (5.08 cm) diameter propeller stirrer rotating at 1000 rpm in a 400 ml tall beaker.

After initial hydration, each sample was diluted to 0.02% using a 0.01 M solution No. C1. This was carried out by weighing 20 g of a 0.2% solution in a 400 ml beaker, followed by the addition of 180 ml of diluent. The diluted samples were mixed for an additional 30 minutes. Final dilutions were prepared from these samples, used directly to determine intrinsic viscosity. Each diutan sample was evaluated at the following concentrations: 0.004, 0.008, 0.010 and 0.012%.

Viscosity measurements were carried out using the Uyazye® UE 8u§1et system. Before measurements, the Uyazye system was calibrated with water with an error of less than 2.0%. Measurements of the viscosity of the samples were carried out using a timer program (T1g ergodgat) at 2 Hz, a voltage of 1 and a shear rate of about 12 l / s; all measurements were carried out at a constant temperature of 23 ° C. For each sample, five measurements were carried out, according to the results of which averaged indicator was derived. The obtained average viscosity values were used to calculate the internal viscosity. In FIG. 2 and in table. 4 presents the final results of these tests.

Table 4

Comparative evaluation of diutan based on calculations of internal (intrinsic) viscosity

Diutan Sample Measured solids Intrinsic viscosity 8657 Control 1 93.76 138.3 8657 Control 2 92.42 143 8657 / p88 Sample 1 91.7 170.7 8657 / p88 Sample 2 91.4 162.2 8657 / p88 Sample 3 91.94 162.8

These results indicate that strain 8657 / p88 continuously produced diutan with significantly higher intrinsic viscosity; in fact, the average reduced viscosity in the case of the strains of the invention was 165.2, while in the control it was 140.7, with the solids content being the same in all cases. This discovery indicates that diutan produced by strain 8657 / p88 has a higher molecular weight than diutan produced by wild strain (control).

In FIG. Figure 2 graphically presents these trends identified between the control and strains of the invention, showing a consistent increase in internal viscosity, the measurements of which were carried out in all cases with the same solids content.

To establish the identity of the compositions of diutan gum with a high viscosity from strain 8657 / p88 and diutan gum from a wild strain, an analysis was performed on the content of neutral sugars and organic acids in them. Purified samples used for measuring internal viscosity were also used for neutral sugar analysis. An aliquot of each purified sample was subjected to hydrolysis with trifluoroacetic acid (100 ° C / ~ 18 h) to sugar components. Quantitative assessment of neutral sugars in hydrolysates was carried out by high resolution anion exchange chromatography with a pulse amperometric detector. Quantification of organic acids in hydrolysates - by high-resolution ion exclusion chromatography with a detector with chemical suppression of conductivity. In the table. 5 summarizes neutral sugar analysis results. As can be seen from the table. 5, the neutral sugar profile in the case of strain 8657 / p88 was almost identical to the neutral sugar profile in the case of wild strain 8657. Although the results in both cases differed from the theoretical values, these results indicate that the structure of the repeating unit of diutan gum produced using the plasmid p88 is the same as in the case of the wild strain (i.e. without plasmid), and that the increase in viscosity reported by the p88 material is due to longer chains, meaning an increase in molecular weight.

- 10 015170

Table 5

Analysis for neutral sugars and organic acids of diutan produced by the p88 strain and wild strain (control)

Strain % ramnose % glucose % acetate Sample 1 8657 / p88 32 nineteen 8.9 Sample 2 8657 / p88 32 nineteen 8.2 Sample 3 8657 / p88 32 17 8.6 Control 1 8657 wild thirty eighteen 8.6 Control 1 8657 wild 33 twenty 8.7 AVERAGE 8657 / p88 32 18.3 8.6 AVERAGE 8657 wild 31.5 nineteen 8.65 THEORETICAL ... 46 thirty 8

Significantly improved indicators of viscosity in sea water and viscosity at a low shear rate with REO for diutan produced by the genetic engineering strain 8657 / p88 may be due to an increase in the molecular weight or length of the diutan molecule, i.e. an increase in the number of repeating units in the molecule, and not a change in the composition of diutane and not a change in the structure of the repeating unit itself. It is impossible to improve rheology only by increasing the amount of diutane produced. Although all four plasmids, p86, p88, pX4, and pX6, with different fractions of the cluster of genes that control diutan biosynthesis, were evaluated, and all of them showed a slight increase in productivity, only plasmid p88 showed an unexpected and very strong increase in the rheological parameters of the recovered diutane product.

A comparative assessment of the genes responsible for the biosynthesis of diutan cloned into the test plasmids suggested that the gene responsible for the increase in molecular weight is most likely the brzO gene, since this gene is present in p88 and not in other plasmids. The brzO gene encodes a hydrophobic membrane protein with a strong homology with other membrane proteins involved in the synthesis of polysaccharides. A portion of the protein is homologous to polymerase-specific proteins, an enzyme that catalyzes the binding of repeating units to form a high molecular weight polysaccharide. It has been postulated that the homologous gene De1O in 860 functions as a polymerase in the synthesis of gellan (Nagbshd.K. e! A1., 2004, Ogdatha! Vyu! EKe, 31: 70-82; 8a-Cogge1a I. e! A1., 2002, Oe11apit. 29: 170176). The brZO homologues were also isolated from strains of 8pShpdotopaz ATCC 31554 and ATCC 21423 producing polysaccharides 888 and 87 (Po11osk! Ra! Ep! 6709845). Therefore, it is highly likely that additional copies of the gene responsible for polymerase can influence the increase in the length of the diutan molecule. It cannot be ruled out that other genes in the cluster of genes that control diutan biosynthesis may be required in combination with brzO to achieve the increase in viscosity observed in the tests. The most likely candidates are the brzV, brzB, brzK and brzR genes encoding transferases of sugars I, II, III, and IV, in particular the brzV gene, which encodes transferase I, which adds the first sugar of the repeating unit to a lipid carrier. The other most important genes may be brzO, brzC and brzE, which are homologs of the ditB and ditC genes, which have been shown to increase the molecular weight of xanthan when amplified on a multicopy plasmid. It is possible that all genes cloned into p88 plasmid may be required to achieve a sharp increase in viscosity.

Although the invention is disclosed in certain preferred embodiments and practical conditions in the present description, this was not done with the intention of limiting the invention to these specific options, but rather with the intention of emphasizing that it encompasses structural equivalents and all alternatives and modifications that may be made in the scope of the attached claims and equivalent to it.

Deposit of strain

The next bacterial strain was deposited on October 21, 2005 at the Patent Depository of the American Type Culture Collection (Ra! Ep! Oerozbogu a! She Atepsap Ture Si1! Ihe Co11ec! Ju) at the following address: 10801 ituez1! U Voi1euagb, Mapazzaz, Va. 20110, in accordance with the Budapest Treaty on the International Recognition of the Deposit of Microorganisms (Vibarez! Tgea! U £ og She! N1egpaIopa1 Kesodpbyup o £ She Oerozb o £ Mugogogdashtz):

8pShpdotopaz strain 8567 with plasmid p88.

- 11 015170

Sequence listing

ZEOIELCE ΙιΙδΤΙΝΟ <110> NagShpd, Yapsu ₽a1, Uap11.n1 <120> ΗΙΟΗ νΐ5003ΙΤΥ ΟΙϋΤΑΝ OTMZ

<130> 05-506, 05-506Ά <160 45 <170> EZEZO ог И п Ц Ц из из из з з 4 4. <210> 1 <211> 26278 <212> MA <213> Sp. ATCC53159 <400> 1 .0 yes Yaaasddo 60 sGGsda ^ a 120 dssEdsbdsd dasdsdasaa aG ^ sdsssXd dksaaed ssasdsdsdsds cEDSSssads edb £ desd £ sdss Ddakdaesd dsssddassb dsdaasde 180 aedaesdssd sdsesdedd ssdddsdds sda dsedssedss sdsdsdsaE 240 sdaaasseee edsdsdsdaa sassddssde C-Sassdssa dsdaddsdsd SESSA 300 ddsEaDddsd dddddsss dsSasdass DEADSADSDA ssdsdsak yes Gdddds 360. sds Bsddsdase esddddass desdssdeda sseseasas ddssssssed 420 saas adsddeaNs dsdsdsss UssdsasGdS ssdssdsdsse d61ssdsdsds 480 sdsssadsd sdasdsdds dddsdsda dsdsessada essssdsds desdsdyas 540 addddsdss e.assddd1: daxdddsdds ddaseaNds Sdddssdss yeah 600 ses ^ ddAsd dsaadsssdss sddssseddsd ddaasddd Ssdsdsesbl СССdeesСес 660 ddsdsabddT ddaeddsED ddsdaessaSS esdeesadsd dssedsadds dadsddd 720 ssdaaadsda dsdsda ssds ssadddds dadsedsas ssd SDDSDGT 780 dsddddakss yes sddassdds dssdsadsdd esetseddd asdsssssss 840 cdd essdidas sddsadds ddsdsssss dssadasdde daadsadea 900 Sssdeaeddsd dsdssdssssss sassd aSdsG.sdg.dd dsdsdassSa Bcdhhhhhhh 960 dsdsdsss ddddsdease gardensdsseds Sdsseddesd sdsddsads

- 12 015170

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- 13 015170

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- 14 015170

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- 15 015170

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- 16 015170

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- 17 015170

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- 18 015170

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- 20 015170

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cskdddsds 17820 saSdSsssd SSDSDDSDA Bsbdsbdbs dadsadsds ADDITION: sddsssssssd 17880 sassbdss Bdsdssbdd ssdsdsdssda dasddssds ssdsdaSDsd adsddssdss 17940 dddssadss DSS dddssadssd ddsdsdddsdsd addssdsda dsdbsdssas 18000 Bsdsssddd sydddsdddsd sadddsdas daadsddaad ssssbdsssd ssdsdsdsss 18060 yesDaSdss SDDSSDDSDSD sdddsd sbdddsdside adsadss ssSssdssbd 18120 Sssdssssss dssdasdasa sdd1: sdd1: s dadsdbda adsass ssSKhdddsdas 18180 ssssssdssss Bsdssssassa ddakdsddsd dasdabssss dbsdddsd asbddasdaS 18240 sddsd ssdaSddsd СДДССДССс cddsdbsdds dsdasdasES sdassssdss 18300 yes saddsdddsdd ДаdaSsssssad sssddssads Асасассъдд sddbaadssd 18360 sdsddddds daaassddss dysda sssadsdsd kindergarten addsddbds 18420 aSaadsdSsd asdsdddsad dsadsasde a bdsdsds sdddsdadsd ddsdssdsd 18480 dsdsa asaasdddsdb Bcdbsdds Alcssss. adssdsssbd dsddsddsds 18540 addSsdsdsa adsddssdss saddsdsaas aaysdisds ddddssds ssdasdabd 18600 sddesssdsb surrender dayddd kindergartens ssdsdsdsad dsdakdsdsd 18660 aSassassad dsddsdds ddssdada dsdsdsaddy Hddbd sadssss 18720 SsddsdaS surrender dddSds Bdaassassa ddabdsdsdd ASSDSSDASD 18780 adsddsddd surrender ssdsbdsdsds • bdsdssdsdd adada £$ das dssdsdsdy 18840 AssisSsdd Bdcdadss dsdsddsjdd yes aaSsaDdsds dssddssads 18900 dsdssdssd sdasdasa £ C cSdaasdds iddsdddd Дddadadds yes 18960 sdda ddsbdaasad gardenBSSbss Kindergarten sdssdasbd dsdasdsads 19020 saddsdddda sdadsbdsds SasdSdass • bcdcdass dsasdsdss dada 19080 addSSdadss dsbddadsad b-dddssads dsdasgds ssdadssdsda sdaassdasd abdssdadsd addDdsssds sdda-bdbs adsdda sds sdddsddsdd 19200 SssSsdsdsaS add up dasnhsgsd aadsdaabsd sassdsdsad sassdddsads 19260 dSDsdssd addssddddsd sdbssass ddaDddbda dsasd sadssdsbdsd 19320 AssdadaSds dassass yeah sasadsSDsd ssaddsdab sassddssssd 19380 dasasdsdsb ddddsdaas dbydaasdss assaddsds sbsdsdsabs sdsdssdssd 19440 aSassassss Bddsdssdaa daasadda dssdsdaads Baaaaaaaaaaaaaaaaaaa

- 23 015170

yesSsadsSsd 19500 bsdsssdds SDSSDD ddsdddSaSSd aSadssdssS dsdasdsdds ddSabdddsd 19560 dsddsdds dssssssadsd ACCCssssssad Sdsdsds ssdsdssdsds sssdassdssd 19620 SddaSdsssd adasdsessss dasdadsads dsdsssdsdsdd sddadsSssSS SssdaasSSd 19680 Sssssssssss dsdsdsddad sddsssdddsd add-on a'asdaSsds aSaddsdaSs 19740 add-on dsassda sdadadsa ss ddsdadGarden asadsasds ddsaaddaas 19800 ^ asdaaad Saaa asadsdddss sassa d-sadsaadd sdsSdaSaad yesaSSdsdsdd 19860 alcdcdcd dsddsddas dsddd dSdSsdsssa sdsdsdsdsss sSdaaaaGarden 19920 dsddsds kindergarten dSddSddaas adssdddsas ssdsssdas dsdsssssss 19980 SdsdSdsSse sdd-ddaasad dsddd aSssadssda dsdssassss ssassassdaa 20040 assdssadda addsdaadds dadsasds adsd Sdsvdssdsd yesSadsassass 20100 SHKDSSDASA sdscssddaa saasadds dsddsdadds сддсаддСЪ dadsdsdadd 20160 ddaSdssda dsassSsdad daasadsd for rent ddaas dddaassad 20220 dadaddssda asssadsdd ssdsssssds assdsdsddd Food sassadsdsd 20280 ssddassada SsdsdSssad sdsdssssdd SsdassSdSX ssdddsda dssdsdddsds 20340 SddaSdaSsa sdsssdsssss dsSsaddssd loan assadsssss dddssdsdssd 20,400 dsda kindergarten dsdddsdadS ssdsdsdsdsd dsassssdas dddssddsd 20460 sdsdssss dsdsddsdsss dddssaddd aSaddSsdS . sddsdsssdsds sdsssssdsa 20520 Eddssadsad sdsddsdsad sddsssddds dSdaSddsda SdSSdEdsDs dssdadsads 20580 adsdasaasd sssassad ddaSdsdsds adssssssssss sdsdsssssds sdssssdd 20640 dssdsdadsd sdsbssdsad dddsddssds a-SSSsdSdss dSdSSaSSSSs sddaassssd 20700 sssssssdds YesSasSupply dsdssa daadaadds ddAaaadasS SASSSAASSS 20760 SADS-SSSSSS ddSaSSSass sdSadsSdss dasssssssss dddasaddss Units 20820 ddSSssSSaaa sSsdassdas BaSassdsda sdssdaddad dddddaddass ddsdsdssaS 20880 sdsdsdsda aasdsdddd ssSdssaasa SSSsdssdda dsdassssds sdsdaadsds 20940 dsassssdsda AsdsaaSdas ddsdssasd saaSSSSDDSS SdaSsssddd sdsdsda 21000 sdaaadssd sssssdddss dssaaaasEc dSsdaaayaaa ssdasaaaas sassdsaeaas 21060 ddsddaSaS SdsaddsSS. DSSSSDSDS Sssdssdsssss aasssssss SdaaSadds 21120 addssads SdassaSdasa GIVE UP SSSddaasdd SASASSSDD

- 24 015170

Ssdasasdda 21180 dasSssdds sdddsaassd Ssddssdssd 21240 Ssdsdsdsdsa saSssdssd daadsasads 21300 dsdsscda ddadsaasss dssssdd 21360 ddsdssdsdsss dasdsddsdd dasadsssssssss 21420 SdaSSSDDS sddssssas ssdssdsdsdd 21480 ” sdddsaaaa ssSddSdsSs cSsdsaadsd 21540 dsassSasSss dsdaasdds ACCSCCSSDD 21600 SALCSS ssdaSsSss ddsddsds 21660 sdsssdsssssd sdsdsdsdsd cSdaSddDdd 21720 dssdssddaS dcssdssds sksdasdaas 21780 SDDSSASSAJ sdasddsdss sSsdasdsds 21840 ddaxaSsaa SssSsSsdssd cDddssassa 21900 ssdSdaSdd dsssdassdd dsdd ssddd 21960 sds sds sds saaddsa SSsaaSdsds 22020 Sdddsdsda sddsd Ssdsadddss 22080 sdsssaasaS dsssaassds assdssassdd 22140 ssdsssdsssdsds dsdddsdssd cSddadadss 22200 sddddssdsdds dsdsssdsds SSsaadaSsa 22260 Squad ssdaSdsds Ssddssadss 22320 dsdasdasda SsdsaSassass aSsdasdaas 22380 SdssdsadsS dsdaasdsd sddsdsds 22440 sdsddddssss dsdsdssds СасСддсасс 22500 dssasasds saadssddds sdsddddsda 22560 csdssdssd sydsssssd alsdasddas 22620 dddaSaSsSd dsdsdaSaS dsdsssssda 22680 asdssssssd SSSDSDSAS ddaSSdasad 22740 sddsssdsds Sssdsssssssss caddddda 22800 Sas bdaa adddsSsaS

SsssssssaSa dSdsaabs asaddssdaa adddadsssd aSdaddsaaS daasdsch-s sdddsdssa sssdddssas dssasssdss aSddSssSSssS aSassdadSS dsdsdsdsdsd dessddsdd dsasdsdsda sddsaas SSssSdsSds sdaSdsdsSSsS sddsassdsd csdsdsssass sdsdssdsdd sdaadads dessssssds Bldg ssdsdsdasd dsddddddsd sdasssssssssss dsdddsssssss sassdssdsdsd sddsassdd sdsdsds Ssdsssdasd Ssdsddsaa SdsdsSdsds aassssdsdsd aaaaaaaaaa dsdsdsssdss dsdaSsdSsd ssSdsassaa ddadsaSDs aasassaadd dsdbssss ssssssssad dsssssdasd ddaaddaSas dsdddsdss dsdaadaads dsdsdsssda BcssdsdaSs SPRING СдддддсдаЪ sskdaSaad sadda Ssddssdsdd saassds dSaasdsDy dsdsdsdaa sddsaasdss aaddSsddss dsSsaSssd saadassads sydsdsdsdsd AsaSdadsds ssSdsdsssd daSassSdS Bcddddaaa surrender Akdassdds SddssaddS dsdsdddsss assaassds Sssaddsad saSddaaSaS asdaSSSSSDS Ssaadas dsdddSdaSs dasds ssdsssssds aaaaaaaaaa sdSSsdSSS SSDSSDDSDS sddssssdsds ccdcdddd ddsadsdda sdsdssjdSa

- 25 015170

ssssdsaasd 22860 saSaDesE give in dsYessssds BaSdasaads give in seaessdsed 22920 Foodstuffs Yessysassdd sassdddas aEssidaEBa EUssasssss dsdsdass 22980 ssdeyidEess addsdsedss ddddsdasds EsdssEesd dsaksaassE sadsya edss 23040 dadsadss sssssaasdd dskddssdaa dsdssabsa Esddsddsda (X-bsddesdds 23100 aasdaissa dsdsds ssddddsdas aasaesheis asddsdaaaa yes 23160 sds edssadd sadssadsads kindergarten saddddsdd saaasdESE sdssaaesai 23220 desdasdass ssdadsdsea sdddsddd dsd sddadasdd sdksdsssass 23280 adsdksdadd aaaadssdds sdadsssaad Qssaaseddd surrender scedeaye 23340 Esdasaadd asddydsda saesdssaad EsdaEssads ssdsdsdsdsd sddsdaasyis 23400 giveysassd asdssaassd dadsdsddsd assedasas sassdsdskss 23460 ddssdsdds aEDSSEDDS !: sdasassds Assad ddsidsad addssdsd 23520 ДЕдЕЕссдддд sds gsddasdds DEAADASD ssidssdsda ddaaaesdss 23580 Eesdaaadsd dseddsedsdd sdsdaadas SEDSESAADS dsdssdssdd ssiddsaad 23640 assddds ssdssjays ssdsaaddy dsdassdsad sided garden DESAYS 23700 asdaasid sddsdesaes dad sesasds sdsssaaaya Edddasas sdsdds-bce 23760 Ec1: ssdaad1: DEEsaadsad Esddydes esd abdssdaadd sydsdadds sdsddds 23820 addasaesa dadsessed dsddssssdd dsasdakssd sdddsdsas sSssaddsds 23880 sdsssEesds ssadsssaad SEDDESSDDSD Unitsddsdd sdsdaxEec dassddsdd 23940 Bsdasayssd Esddsdsd sssasska dsaaayddd sdddsdksdad cccssdddss 24000 adaaddadaa ssadtsedsed dkssssdssd dsEydsds sddsEesaed asdsdbs 24060 surrender daxxcas addxadsad csaaaaaa daadda dadddsda 24120 СсссдЕЕдддддадад Yessda): syssssaSd ssdddssdda saxdddd dadssddcss 24180 EsEssdaaaa dddsddx dssasssss Esdssdzael saasassssss EECESSECax 24240 addds edads caEdsadsad ASSESSSSD Esassddsdd sdssdsds aEddSysDd 24300 sdddddsd ssasssdes sdssadddsd ssdsdsd saaescdas Aads Esassay 24360 iddsddsaa sssddssssd SEDASEDSDA Osdadaasds dsssaac sdsEsdesDess 24420 iddas sdssdasass dsdasdayss EassdsEdsH dsdsdaddad saddyside Idddadgs csdssdss dadadss ДадаййдддсЕЕЕ dysdasds

24480

- 26 015170

sskdddsdk 24540 xaxdadas saakdsks ddsassks adscssssss dksdddsdcd saakkddds 24600 dsdadskda ddddsdaaaa sdsdsdsdk kssdskssa ssasaksksss Assdasdaad 24660 cdcdsddsda sskddssdkks dasadsds kskksassda adadasssss kakdaksssk 24720 ssksdssska kksdddsdksd. aadsddsda dsdassak dddcdsdss cddddssas 24780 skskdddsk dssdddcdcd skddksdaask dsksdaasaa kkasddddssd kkssaskkss 24840 ssdadaadsk daxssdcd assax asdsdsdsda ddddsaadssd skddssddsk 24900 addsdaadd surrender sdsdaskds cdcdcdsda sdaksasdss aadsddd 24960 sdassaksds sassassdds addxdddss adadskasaa kdksdddsdds sdsaasdads 25020 ddassaass dsddcddx dadasdaksk dsdasskdsk sdassadds accssdcdd 25080 ssdasddsd saadsdssds daaskdax skksdksas sdaksdssss ddssakdass 25140 dssdskasds daxdasd assaads adassdadsk ddddsddaad dskdaddada 25200 3 and u and Su a Co. FROM SuuSaiSouss usdiyod aiuuassi ddedaasdad cdddddddd 25260 dsssdakssd skssdsdsaaa kakdssddsd adsdddsddd dsadassdss kdakdsdkak 25320 sksksdsksass dddsakdasd dssaddsds saddle dssdadsadd sddcddddss 25380 sdadskddks kxassassk assssdaack sdksksksss aadssddada saksdadds 25440 sddcdcddsd sddddd sddsksk sdksksssss dssdsskasa sdddsdksda 25500 saadsddaa adsdaasssd hell ddsdaksaas ddsdasdds ssdddsdkdsk 25560 ddsdsdsdsdsd ddsdsdaada Bsddsdsdss daxaxassas skdksdassd accaccdccc 25620 surrender SKSSDSSDSS skkdddsda ddasdaksss assddssdsds csddsda 25680 cdddsdass aadskddssd dsdadsadds dddssadds ksdddkdssa ssaasdsdk 25740 dakssddskd dskdsdddsk asadsssdkk sddsaasaak Kksdksaada sdakdskssd 25800 ssksdsssdad asdsdsdasd sdskdaasdk sdkddaddas sadkddddsk dssssadkks 25860 ddsdsddd axdsdassd sdaksskdas ddddddd sucksdads addasddsds 25920 dasdaddsdds skskassakk csdsddsas sddsdadass aaskdddssd ascksdsax 25980 dasdaxx dssdadad ssaadsdsdd cddssssdsd dssassdks ssdsdsksss 26040 sadskdsdds kakssdasks sddssssdsd sssddsssaak ksdsdskdd asksdsssd 26100 skksdsddad asksksddsk assdddsdss kdsskdsdsad daxxdskda addxdask ddaksdskds skdsdskdak ssdaaasddd dddssksads dssssssds

26160

- 27 015170 a Ssdsbsssdb Ssdsdsdsdsds dsaabdsbss Sarsassdsdsd sdssssssssb Saddasbsad 26,220 sbsdsbssad ssdsdssssss ssbbsddsda ssdsdsasas sssadsdsas ddsdsds

26278 <210> 2 <211> 1053 <212> ΟΝΑ <213> ATCC53159 <220>

<221> POPs <222> (1) ... (1053) <223> brzZ (rugbha!) <400> 2

abs aas 48 dds dss bbd sbs dda 61 y sdd sas aah Bz 10 66s dbe ebd dbe aab grandfather Ya1 Bie 5 Bie Agd H1z Not one Azp 61y A1a Ръе Ya1 Ley Wa1 Azp fifteen Sss asd dbe dss bsb Ssd aba ebd Sbs garden ebd Sbs ssd ebb dbe dss 96 Zeg Tht Ya1 A1a Zeg Zeg Not Bie Ръе 61p Bie Ръе Rgo Bie Ya1 Ya1 twenty 25 thirty dss bdd abd abs dds ssd das ebd food asd ebd ebd abs dss grandfather sbs 144 A1a Tgr Meb Not 61y Rgo Azr Bie Agd Tht Bie Bie Not A1a A1a Bie 35 40 45 dbb dds sdd grandfather dbb ssd abd abs dds abd ebd sss grandfather ebd Sub food 192 Ya1 C1u Agd A1a Ya1 Rgo meb Not C1u Meb Bie Rgo A1a Bie Tug Agd fifty 55 60 aas ebb bbd sds dds aas asd ssd edb STS sas dss hell dad grandfather sds 240 Azp Bie Bie Agd C1u Azp TNG Rgo Agd Ръе H1z A1a Zeg 61and A1a Agd 65 70 75 80 66s ebd aba dds women dds ddd SDD dss Ssd sbs asd ace grandfather dsa grandfather 288 Pb Bie 11th 61y Tug 61y C1u Tgr A1a Zeg Bie bj bj Ya1 Ya1 A1a 85 90 95 ace dbd sbs abd abd grandfather das sds 66s ebd abb dds dsa ebb ebb ddd 336 Bj Ya1 Bie Meb Meb A1a Azr Agd Ръе Bie Not 61y A1a Bie Bie 61y one hundred 105 110 sss dbe dss dad ace abs Sas asd dss sss ebd saa sbs dsa garden sds 384 Rgo Ya1 A1a Ya1 TNG Not Tug TNG A1a Rgo Bie 61p Bie A1a 61p Agd 115 120 125

- 28 015170

dSa 432 Wa1 Sed ssd ssd sss Sss Zeg dsa A1a 135 ssd sss dss grandfather sed sss ssd sds Agd eat Bie Zeg 130 Bie Bie Rgo Bie Zeg A1a A1a Bie 140 Pb Rgo sss hell grandfather asd ssd grandfather dad sds ASD grandfather STS garden ACC sds Ssd eat 480 Rgo Zeg A1a Tht Rgo A1a 61and Agd Month A1a Bie C1p Not Agd Zeg Bie 145 150 155 160 Ssd csd ACC asd dds dds ECC ace ddd ASD ACC dds dds dda cSa SSD 528 Zeg Bie Not MeC 61y 61 y Bie Tht 61y MeC Not 61 y 61y 61y Bie Bie 165 170 175 ssd dss grandfather ssd ECC ssc yes ssc SDD ACC dds aad Ssd cEC dds sae 576 Bie A1a A1a Rgo Pb Bie Azr Bie Tgr Not C1u Bz Zeg Bie 61y H1z 180 185 190 grandfather dda asd ssd des grandfather cEC SSc csd SSc SSc dds dsa SDD dd aaS 624 A1a C1u Tht Rgo Ya1 A1a Bie Pb Bie Pb Pb C1u A1a Tgr Tgr Azp 195 200 205 grandfather csd grandfather ACC ACC Sed EUs hell dds csd garden grandfather hell dda sdd ssd 672 A1a Bie A1a Not Not Zeg Pb Zeg 61y Bie 61 η A1a Zeg C1u Agd Rgo 210 215 220 aah grandfather hell grandfather ACC dss garden ddd dsa dad csd cSa sss d ^ d SSD ACC 720 Bz A1a Zeg A1a Not Ya1 61p 61y A1a C1 and Bie Bie Rgo Ya1 Bie 11th 225 230 235 240 grandfather csd CaS dsa ddd ACC food SDD dds dds dsd ace dds dss dsa grandfather 768 A1a Bie Tug A1a C1u Not Agd Tgr 61y C1u Ya1 Tht 61y A1a A1a A1a 245 250 255 dss STS eat dda sds Sss dss csd yes SSc dss eat SSD ace ^ dd garden 816 Ya1 Pb Bie C1u Agd Zeg A1a Bie Azr Pb Ya1 Bie Bie Tht Tgr 61p 260 265 270 dsa dds csd ssc sds garden asd grandfather aad saa dsa Sss dSa Vds dds dss 864 A1a C1u Bie Bie Agd 61p Tht Ya1 Bz 61p Ya1 Zeg Ya1 Suz 61y A1a 275 280 285 DSS ssc ace dss grandfather ASD ssc dsd dds grandfather ace EAE sds CaS Ssd d ^ d 912 Ya1 Bie Tht Ya1 A1a MeC Bie Yb 61y A1a Bj Tug Agd Tug Zeg Ya1

290 295 300

- 29 015170

ssd cEC Unit Food yeah SCS hell dss Food Food eat des grandfather eat dsa dss 960 Rgo Bie Tgr Suz Yb Bie Zeg A1z Suz Suz Bie Yb Aba Bie Aba Aba 305 310 315 320 Food Ess Unit Unit asa Food grandfather sds garden das aad dsa eat with food aee dda

1008

Suz 5eg Tgr Tgr Tkg Lei Aa Agd Sb Azr Luz A1a Bie Bie Not ce 335 325 330 food 1053 Food hell food acc ska ssa aad garden edd saa SCS das ska Ed Agd Bie Zeg Agd Not Bie Rgo Bj b1p Agd C1p Bie Azr Bie * 340 345 350

<210> 3 <2Ы> 350 <212> RKT <213> ATCC53159 <220>

<221> REVERTE <222> (0) ... (0) <223>

Not Azp C1u one Aba Еи еи у 61 61 Аг д Н Н з уз уз Rke Yb Bie Yb Azp fifteen Yb 5 10 Zeg Tkg Yb Aba Zeg Zeg Not Bie Rke Bn Bie Rke Rgo Bie Yb Yb twenty 25 thirty A1a Tgr Mek Bb Sue Rgo Azr Bie Agd Tkg Bie Bie Bb Aba Aba Bie 35 40 45 Yb Sue Agd Aba Yb Rgo Mek Bb Sue MeE Bie Rgo Aba Bie Tug Agd fifty 55 60 AZP Bie Bie Agd Sue Azp Tkg Rgo Agd Rke Hb Aba Zeg SI Aba Agd 65 70 75 80 Rke Bie Bb Sue Tug Sue Sue Tgr Aba Zeg Bie Tkg Tkg Yb Yb Aba 85 90 95 Tkg Yb Bie Mek Mek Aba Azr Agd Rke Bie Bb Sue Aba Bie Bie Eu one hundred 105 110 Rgo Yb Aba Yb Tkg Bb Tug Tkg Aba Rgo Bie Bn Bie Aba Bn Agd 115 120 125 Yb Zeg Bie Bie Rgo Zeg Aba Bie Zeg Aba Aba Bie Rke Rgo Agd Bie B3 135 140 Rgo Zeg Aba Tkg Rgo Aba SI Agd Mek Aba Bie 6bn Bb Agd Zeg Bie 145 150 155 160 Zeg Bie Bb Mek Sue Sue Bie Tkg Sue MeE Bb Sue Sue Sue Bie Bie 165 170 175 Bie Aba Aba Rgo Rke Bie Azr Bie Tgr Bb Sue Bz Zeg Bie Sue Hb 180 185 190 Aba Sue Tkg Rgo Yb A1a Bie Rke Bie Rke Rke 61y Aba Tgr Tgr Azp 195 200 205 Aba Bie Aba Bb Bb Zeg Rke Zeg 6y Bie Bn Aba Zeg Sue Agd Rgo 210 215 220 Bz Aba Zeg Aba Bb Yb Bn Sue Aba SI Bie Bie Rgo Yb Bie Bb 225 230 235 240

- 30 015170

A1a Bie Tug A1a C1u 245 11th Agde Tgr C1u C1u 250 C1u A1a A1a 255 A1a Ya1 Pb Bie C1u Agd Zeg A1a Bie Azr Pb Ya1 Bie Bie Tht Tgr C1p 260 265 270 A1a b1u Bie Bie Agd C1p Tht Ya1 Bz C1p Ya1 Zeg Ya1 Suz C1u A1a 275 280 285 Ya1 Bie Tht Ya1 A1a MeE Bie Ya1 61y A1a Tht Tug Agd Tug Zeg Ya1 290 295 300 Rgo Bie Tgr Suz Ya1 Bie Zeg A1a Suz Suz Bie Ya1 A1a Bie A1a A1a 305 310 315 320 Suz Zeg Tgr Tgr Tht Bie A1a Agd C1g> Azr Bz A1a Bie Bie Not C1u 325 330 335 Agd Bie Zeg Agd Not Bie Rgo Bz 61p Agd C1p Bie Azr Bie

340 345 350 <210> 4 <211> 1626 <212> ΟΝΑ <213> Sp. ATCC53159 <220>

<221> pops

<222> (1). . <223> brzs . (1626) <400> 4 aU asd acE ace Esd grandfather 555 cde sds ssd dss EEC sas dda grandfather aU 48 MeE Tht Tht Tht Zeg A1a Pb Agd Agd Rgo A1a Pb Nhz C1u A1a MeE one 5 10 fifteen garden sdd CEE sds add Food food eat DEER sdd 555 eat asa aad ssa grandfather 96 C1p Agd Bie Agd Agd Bie Agd Bie Ya1 Agd Pb Bie Tht Bz Rgo A1a twenty 25 thirty aec ssd yeah eat sss des Eas aah dss dad food Esa dds grandfather asd aec 144 Not Rgo Ya1 Bie Rgo Ya1 tug Bz A1a C1 and Agd Zeg C1u Ya1 Tht Not 35 40 45 grandfather grandfather sdd cde ace DEER eat eat des hell dbd aU Eee CEE dss dsa 192 A1a A1a Agd Agd Tht Ya1 Bie Bie Wah! Zeg Ya1 MeE Pb Bie A1a A1a fifty 55 60 des bass dds eat cEC dss dsa d £ b eat ssd cEC garden aU eat grandfather aec 240 Ya1 Tug C1u Bie Bie A1a A1a Ya1 Bie Rgo Bie C1p MeE Bie A1a Not 65 70 75 80 ssd dss dd sss cEC DEER eat ASD grandfather cEC yeah grandfather aec Unit grandfather cea 288 Rgo A1a Ya1 Rgo Bie Ya1 Bie MeE A1a Bie Ya1 Ya1 Not Tgr A1a Bie 85 90 95

- 31 015170

sss 336 Rgo dad grandfather edd garden grandfather sss ACS SDS SSD ssd dsa aah Bz cSa Bie 110 Sas Tug ssc Bie 61and A1a Agd one hundred Bie A1a <31p A1a Rgo TNG Agd 105 Bie dss CaS ASD dsd grandfather grandfather ssc dsd SDD ssd aas CaS CCC grandfather ssc hell 384 A1a Tug MeC Ya1 A1a A1a Bie Ya1 Tgr Rgo Azp Tug Bie A1a Bie Zeg 115 120 125 DSS dds dds SSD ssS edd ACC Ssd ACC add sdd ASD ACC dds Ssd ACC 432 Ya1 C1u 61y Bie Rgo Tgr Not Zeg Not Agd Agd MeC Not <31 y Zeg Not 130 135 140 dsa SSD ssc asd ec ASD ACC Ssd see Ssd dss Ssd aah aad eee food 480 A1a Bie Bie TNG Bie MeC Not Zeg Bie Zeg Ya1 Zeg Bz Bz Pb Agd 145 150 155 160 Sss dad ASD grandfather grandfather ACC ASD sdd grandfather grandfather ssd aes ssa Ssd food cSa 528 Zeg C1 and MeC A1a A1a Not MeC Agd A1a A1a Rgo 11th Rgo Zeg Agd Bie 165 170 175 SSD ess dss SSc ACC ASD dss garden ACC dss dss Sss ace grandfather ass ssa 576 Bie Bie A1a RNe 11th MeC Ya1 61p Not Ya1 A1a Zeg 11th A1a Tht Rgo 180 185 190 grandfather dss Sed saa asa ACC ssd sdd sed ace dds aes DSS csd asd grandfather 624 A1a A1a Zeg C1p TNG 11th Rgo Agd Bie Not C1u 11th Ya1 Bie Tht Ya1 195 200 205 asd ssd ASD dsa SSc ACC Ssd cSa dd csd ACC ddd ass das asd sds 672 TNG Rgo MeC A1a Pb 11th Zeg Bie Tgr Bie Pe 61y TNG Azr Tht Agd 210 215 220 ass ssd dad SDD SDD des asd sds SSD see edd Vds dsd dds dsd csd 720 TNG Rgo C1 and Tgr Tgr Ya1 TNG Agd Bie RNe Tgr Suz Ya1 61y Ya1 Bie 225 230 235 240 ASD grandfather ACC dds grandfather csd dad SSc sdd grandfather aad sas dss ssc ddd dss 768 MeC A1a Not C1u Ya1 Bie C1 and RNe Agd Ya1 Bz H1z Ya1 Bie Tgr A1a 245 250 255 Sas Sed ACC sed hell eee csd sds DSS yes dad garden eee see ass dSa 816 Tug 5eg Not Rgo Zeg RNe Bie Agd Ya1 Azr B1i C1p RNe Bie Tht Ya1 260 265 270 grandfather ECC asd sss dd ^ eee sdd dds ace Sas sds des sed asd ac eee

864

- 32 015170

Ya1 Bw 275 Rgo b1u Pb Agd C1u 280 Tg Tug Agd Ya1 Bie 285 Tht Tht Pb hell hell ssd bb dc bdd dda dad Bd asd ds Bd asd ab css Bc 912 8eg Zeg Rgo Bie Ya1 Tgr C1u C1 and Bie Tht A1a Bie Tht Not Rgo Pb 290 295 300 dad cd sas sd aba grandfather aab Bss sd dds dha ddd hell Bd dsa B 960 Ya1 Bie H13 Agd Not A1a Azp Zeg Agd C1u Ya1 C1u Agd Bie A1a Pb 305 310 315 320 SDD ab Bc Bc yes b C71 quack ds grandfather Bss dda B bb hell ddb

1008

Tgr Not Pb Pb Azr 325 Pb Bie Ya1 Ya1 A.1a 330 Zeg C1u Rie lei Zeg C1u 335 Bc food cd dsa ab dd ddd dda Bd grandfather grandfather sa ace grandfather Ba Bd 1056 Zeg Agd Bie A1a Me Ya1 C1u C1u Bie Ya1 A1a H1z Tht Ya1 Tug Bie 340 345 350 bb ab Dd dsa ab edd edd Dd edd ace asa aad dds ddd bb dc

1104

Bie Not Tgr 355 A1a Not agde Agd Tgr Agd Tg Thb bz C1u C1u bie Ya1 360 365 dds ab Bcd cd asd bld asd Ba sss dss Bd ab dd grandfather cd Bcd 1152 61y Not Zeg Bie Tht Bie Tht Tug Rgo A1a Bie Me Ya1 A1a Bie Zeg 370 375 380 cd grandfather dc ab Bc dd css dss dc sas aas sd bb ss dds dda

1200

Bie 385 A1a Ya1 MeB Pb Ya1 390 Rgo A1a Ya1 H1z Azp 395 Agd Ya1 Bie C1u 31u 400 dds dcb Bc saa bb hell aab saa dds add garden dad garden B sds Bd 1248 C1u A1a Zeg C1p Bie Zeg Azp C1p C1u Agd 61P C1 and C1p Pb Agd Bie 405 410 415 dds dd css dss abc dcb sds sds css Bc Bc dds Ba dds sed dda

96

C1u Wa1 Rgo A1a Not A1a Agd Agd Rgo Pb Pb C1u tug 61y Rgo C1u 420 425 430 give dd dcb dds dcb dc ddd dd sd aab garden saa dds Bc ba Bcd 1344 51i C1u A1a 61y A1a Ya1 C1u Tgr Agd Azp C1p st 61y Pb Bie Zeg 435 440 445 ab das hell ddb Bc cd Bss dha grandfather ds give Bac dds Bd cd dd 1392 Not azr Zeg C1u Pb Bie Zeg Ya1 A1a A1a Azr Tug C1u Bie Bie 61y

450 455 460

- 33 015170

HER Esd cea Eas dds asd aU aee asd EEA aU aee Food cEC grandfather 1440 Rie oub Zeg Bie Tug SBU Tht MeE bbe Tht Bie MeE bbe Bie Bie Aba 465 470 475 480 HER ddE CEE aad aU hell dda yes dds bass ssd CEE dad cEC dss 1488 Rye Agde SBU Bie Bz MeE Zeg SBU Azr SBU Tug Rgo Bie Sbi Bie Aba 485 490 495 dea dsa acE EEC eat DSE DEER CEE EEA aaE asd sdd Esa des eat Esd 1536 Oub aba Tht Pb Bie A1a OUB Bie Bie Azp Tht Agd Zeg OUB Bie Zeg 500 505 560 saa dde das aaE das ssd EEC aec Eee aU asd eat ddd cea dds aaa 1584 S1p b1u Azr Azp Azr Rgo Pb bbe Pb MeE Tht Bie SBU Bie SBU bbe 515 520 525 grandfather CEE cea EAE sds ECE sdd CCE grandfather ECE Food Bcd dd Ed 1626 Aba lei Bie Tug Agd Zeg Agd Rgo OUB Zeg Bie Zeg OUB * 530 535 540

<210> 5 <211> 541 <212> RKT <213> ATCC53659 <220>

<221> RETURN <222> (0) ... (0) <223> riEaEbue robutegase <400> 5

MeE Tht Tht Tht Zeg Aba Pb Agd Agd Rgo Aba Pb Nbz SBU Aba MeE b 5 60 65 Sbp Agd Bie Agd Agd Bie Agd Bie OUB Agd Pb Bie Tht Bz Rgo Aba twenty 25 thirty bbe Rgo OUB Bie Rgo OUB Tug Bz Aba Sbi Agd Zeg SBU OUB Tht 16th 35 40 45 Aba Aba Agd Agd Tht OUB Bie Bie OUB Zeg OUB MeE Pb Bie Aba Aba fifty 55 60 OUB Tug SBU Bie Bie Aba Aba OUB Bie Rgo Bie Sbp MeE Bie Aba bbe 65 70 75 80 Rgo Aba OUB Rgo Bie OUB Bie MeE Aba Bie OUB OUB bbe Tgr Aba Bie 85 90 95 Rgo Sbi Aba Agd Sbp Aba Rgo Tht Agd Bie Bie Aba Bz Bie Tug Bie 600 605 660 Aba Tug MeE OUB Aba Aba Bie OUB Tgr Rgo Azp tug Bie Aba Bie Zeg 665 620 625 OUB SBU SBU Bie Rgo Tgr bbe Zeg bbe Agd Agd MeE bbe SBU Zeg bbe 130 635 640 Aba Bie Bie Tht Bie MeE bbe Zeg Bie Zeg OUB Zeg Bz Bz Pb Agd

145 150 155 160 Zeg SI Me A1a A1a Not Me Agd Aba Aba Rgo Not Rgo Zeg Agd Bie 165 170 175 Bie Bie Aba Pb Not Me Ya1 C1p Not Yb Aba Zeg Bb Aba Tht Rgo 180 185 190 Aba Aba Zeg C1p Tht Not Rgo Agd Bie Not Sue Pe Yb Bie Tht Yb 195 200 205 Tht Rgo Me Aba Pb Not Zeg Bie Tgr Bie 1b Sue Tht Azr Tht Agd 210 215 220 Tht Rgo SI Tgr Tgr Ya1 Tht Agd Bie Pb Tgr Suz Yb Sue Yb Bie 225 230 235 240 MeE Aba Not Sue Ya1 Bie SI Pb Agd Yb Bz Hb Yb Bie Tgr Aba 245 250 255 Tug Zeg Not Rgo Zeg Pb Bie Agd Ya1 Azr SI Bn Pb Bie Tht Yb 260 265 270 Yb Bie Tht Rgo <E1u Pb Agd C1u Tht Tug Agd Yb Bie Tht Tht Pb 275 280 285 Zeg Zeg Rgo Bie Yb Tgr Sue C1 and Bie Tht Aba Bie Tht Not Rgo Pb 290 295 300 Ya1 Bie H1z Agd Not Aba Azp Zeg Agd Sue Yb Sue Agd Bie Aba Pb 305 310 315 320 Tgr Not Pb Pb Azr Pb Bie Wow Ya1 Aba Zeg Sue Pb Bie Zeg Sue 325 330 335 Zeg Agd Bie Aba Me Yb 61y C1u Bie Yb Aba Hb Tht Yb Tug Bie 340 345 350 Bie Not Tgr Aba Not Agd Agd Tgr Agd Tht Tht Bz Sue Sue Bie Yb 355 360 365 Sue 11th Zeg Bie Tht Bie Tht Tug Rgo Aba Bie Me Yb Aba Bie Zeg 370 375 380 Bie A1a Yb Me Pb Yb Rgo A1a Yb Hb Azp Agd Yb Bie Sue Sue 385 390 395 400 61y Aba Zeg C1p Bie Zeg Azp Bn C1u Agd Bn SI Bn Pb Agd Bie 405 410 415 C1u Yb Rgo Aba Not Aba Agd Agd Rgo Pb Pb 2y Tug Sue Rgo Sue 420 425 430 61and C1u Aba C1u Aba Yb e1u Tgr Agd Azp Bn Bn Sue Pb Bie Zeg 435 440 445 Not Azr Zeg Sue Pb Bie Zeg Ya1 Aba Aba Azr Tug Sue Bie Bie Sue 450 455 460 Pb Yb Zeg Bie Tug C1u Tht Me Not Tht Bie Me 11th Bie Bie Aba 4 65 470 475 480 Pb Agd Sue Bie Bz Me Zeg C1u Azr Sue Tug Rgo Bie SI Bie Aba 485 490 495 Ya1 Aba YOU Pb Bie A1a Yb Bie Bie Azp Tht Agd Zeg Yb Bie Zeg 500 505 510 61p Sue Azr Azp Azr Rgo Pb Not Pb Me Tht Bie 61y Bie Sue 11th 515 520 525 A1a Bie Bie Tug Agd Zeg Agd Rgo Yb Zeg Bie Zeg Yb 530 535 540

<210> 6 <211> 1998 <212> ϋΝΑ <213> ATCC53159 <220>

<221> pops

- 35 015170 <222> (1) .., (1998) <223> sArzV.

<400> 6 ' akd 48 MeE one sed yes aec 11th AEE DE DES AAD aaE garden asd dad aac Azp Dec A1a dsa A1a fifteen ax 11th TA g 10 61and Bie Rgo Azr 11th Wa1 5 Bz Azp C1p grandfather 96 grandfather grandfather aad ddE dds yeah ace aec aad Skk dss dss ddd dke cas A1a A1a A1a Bz twenty 61y C1u 61and TNG Not 25 Bz Bie A1a A1a 61u Wa1 thirty Tug asa 144 yes CEC acE yeah aU ace aad asd Eee ace hell akd dkd asa acc TNG Azr Bie 35 TNG Ya1 MeE TIG Bz 40 TNG RNe TNG Zeg Mek 45 Ya1 TNG 11th dad 192 Esd cEC das Esd Esd aas ssd des aac aec saa aad skd dkd ax C1 and Zeg fifty Bie Azr Zeg Zeg Azp 55 Rgo Ya1 Azp 11th C1p 60 Bz Bie Ya1 11th ddd 240 aas hell hell aas DEER ace des aah aas akd dke Dec grandfather ace duck C1u 65 Azp Zeg Zeg Azp Ya1 70 TNG Ya1 Bz Azp MeE 75 Ya1 A1a A1a TNG Azr 80 Eas 288 aad sss dss yes das cdd aaE food eat aac asd ax garden ddk sneaker Tug Bz Rgo A1a Azr 85 Azr Tgr Azp Agd Bie 90 Azp TNG 11th C1l C1u 95 Zeg dss 336 aas aec dkk Food das dds grandfather edd EEC hell dds dds ask ddk das A1a Azp Not Ya1 one hundred Bie Azr 31u Ya1 Agd 105 RNe Zeg C1u 61y TNG 110 C1u Azr CCE 384 Esd cEC Esd aad ddd grandfather dds Food EEC dkd sds aas hell asd sneaker Rgo Zeg Bie 115 Zeg Bz 61y A1a C1u 120 Bie Pb Ya1 Agd Azp 125 Zeg TNG Zeg grandfather 432 asd aU garden aaE Esd ECE aec das sas cx dd £ skd dds ekk dad Ya1 TNG 130 MeE C1p Azp Zeg Zeg 135 11th Azr H15 RNe 61y 140 Bie C1u Bie C1 and dss 480 Eas aas des yes dds aU grandfather des garden aas hell hell cx sas das A1a 145 Tug Azp Ya1 Azr 61y 150 MeE Ya1 Ya1 <31p Azp 155 Zeg Zeg RNe Shz Azr 160 aas 528 edd sds yes sae asd aas EEC acE dad akd aas aac ekk dke ax Azp Agd Agd Azr H1z TNG Azp Pb TNG C1 and Mek Azp Azp Bie Ya1 Pe

- 36 015170

165 170 175 das dda aac sneaker cx asd aas skd kkk sss grandfather dds ace yeah sack sss 576 Azr Sue Azp Zeg Pb TNG Azp Bie Pb Rgo Yb C1u Tht SI H1z Rgo 180 185 190 das dss acc garden cx cx asd grandfather dds aad dke aad dds aac ace aas 624 Azr A1a Not Bn Pb Pb TNG A1a C1u Bz Yb Bz Sue Azp Tht Azp 195 200 205 ax ace ax css aac aas dke ax akd garden dds grandfather dds cake dds grandfather 672 Not Tht 11th Zeg Azp Azp Yb Bb Mek Bn Sue Aba Sue Zeg Sue A1a 210 215 220 saa ddd acc cx akd aac das dad dss dd aac ekk sss as dke aac 720 Bn 61y 11th Pb Mek Azp Azr SI Aba 61y Azp Bie Rgo Tug Yb Azp 225 230 235 240 dka aas ax aah aas aac ekk ax as skd aac ddk as cas sas dd 768 Yb Azp 11th Bz Azp Azp Bie Not Tug Bie Azp Sue Tug Tug ΗΪ8 Sue 245 250 255 ax aas dkk dkk aas dds dkk aac dke aac ax yeah css aac hell dkd 816 Not Azp Yb Yb Azp Sue Yb Azp Yb Azp Not SI Zeg Azp Zeg Ya1 260 265 270 aka sneaker saa dkd duck dds asa ksa kkk cdd acc sds SCS das aah ace 864 Not Zeg Bn Yb Azr s1u TNG Zeg Rie Tgr Not Agd Bie Azr Bz Tht 275 280 285 aac dds grandfather asd ax aad aas aac dkk grandfather das skd ax ace dke asa 912 AZP Sue Aba TNG 11th Bz Azp Azp Yb Aba Azr Bie Not Tht Ya1 Tht 290 295 300 hell css sneaker hell aac ax dyd garden asa dds aac caustic asd skd asd adk 960 Zeg Zeg Zeg Zeg Azp 11th Yb Bn Tht Sue Azp Agd Tht Bie Tht Zeg 305 310 315 320 das sneaker dsa asd ax sds aad ax as dds SCS aas Dan ddd Dec asd

1008

Azr Zeg Ab Tht Bb 325 Agd Luz Bb Tug Sue Le Azp Azr Sue Al Ha Th 330 335 grandfather edd SCS hell duck ccd ax dkk sss dds dke ddd cas garden ssd sss 1056 Aba Agd Bie Zeg Azr Bie Bb Yb Rgo Sue Yb Sue Tug Bn Rgo Rgo 340 345 350

- 37 015170

DED LSD ADE more A1a dee dss dsb A1a A1a A1a garden dd as ace as yeah Bcd ace grandfather Zeg 'Gb A1a 1104 Wa1 Zeg Zeg 355 C1p 360 Ya1 Tht Tht 61and Bie 365 aad grandfather 1152 dsa aab ssd Sed sed sed cbc yes csd Sed ees hell aas hell Bs a1a A1a Azp Rgo Zeg Bie Bie Bie Azr Bie Zeg Pb Zeg Azp Zeg 370 375 380 dds ds 1200 dkd das bb Bcd sas SDD aab ace dds garden asd asa aad grandfather 61u Wa1 Ya1 Azr Bie Zeg Ηίδ Tgr Azp Tht 61y 61p Tht Tht Bz A1a 385 390 395 400 dd das 1248 grandfather Sed grandfather dc dd dds hell dss Bcc sas dc Bcd asd dds Wa1 Azr Ya1 Zeg A1a Ya1 Ya1 C1U Zeg A1a Pb ΗΪ3 Ya1 Zeg Tht C1u 405 410 415 asd ddd 1296 dsd yeah sia aas edd hell Sae Bcd edd garden ab Sae dsa eat Tht C1y Ya1 C1 and Bie Azp Agd Zeg Tug Zeg Agd C1p Not tug A1a Bie 420 425 430 Bfd grandfather 1344 Bc asd cbc hell Bcc das cbc aad edd das Bcd dcb asd dss Zeg A1a Pb Tht Bie Zeg Pb Azr Bie Bz Agd Azr Zeg A1a Tht A1a 435 440 445 asd dss 1392 ddd saa ab bb dds ace ees garden hell Dd Sed more Bcd sed Th1A1a C1u 61p 11th Bie C1u Not Pb C1p Zeg Tgr Zeg Ya1 Zeg Bie 450 455 460 garden dss 1440 aah ddd yeah cd hell ees ace ab sds aas dss grandfather dds dc C1p A1a Azp C1u C1 and Bie Zeg Pb Tht MeC Agd Azp A1a A1a 01 y Ya1 465 470 475 480 ade garden 1488 asa ab grandfather asd hell dds dss aad sed cs yes more dss asa Zeg C1P Tht Me Ya1 Tht Zeg b1u A1a Bz Bie Bie Azr A1a A1a Tht 485 490 495 sas aad 1536 ax dss sed ace Sae das hell asd edd aah ace grandfather aye sed H18 bz Not A1a Bie Tht Tug Azr Zeg Tht Agd Bz Tht A1a Not Bie 500 505 510 Race d'a 1584 das dds ab saa sds dds asa grandfather asd ab ace dds asd ace Tug Wa1 Azr C1u Me C1p Agd C1u Tht A1a Tht MeC Tht C1u Tht Tht 515 520 525 edd sss dss yeah Bie Dd ddd cd baS dc dds hell ssd ie Bcd ace

1632

- 38 015170

Agd Rgo A1a 530 C1 and Zeg Tgr C1u Ley 535 Tug Ya1 C1u Zeg 540 Rgo Rke Zeg Tkg dsa Bbs hell dda asd dss dds das ACC dad ACC eating das dds grandfather ACC 1680 A1a Rke Zeg C1u Tkg Ya1 C1u Azr Not C1 and Not Agd Azr C1u A1a Not 545 550 555 560 hell dss dss garden dbd garden grandfather eBd dkd ace grandfather Sed hell dss hell grandfather

1728

Zeg A1a A1a C1p Ya1 565 C1n A1a Ley Ya1 Tkg A1a 570 Zeg Zeg A1a Zeg 575 A1a grandfather grandfather asd dB s aad das hell sbs dke ace dds grandfather dss grandfather garden dss 1776 A1a A1a Tkg Ya1 Bz Azr Zeg Bie Ya1 Tkg e1u A1a A1a A1a C1p A1a 580 585 590 dss grandfather csd eBd grandfather ddk dss dds dss dss hell asd dsa asd ssd eBB

1824

A1a A1a lei 595 Bie A1a C1u A1a C1u A1a A1a 600 Zeg Tkg A1a 605 Tkg Rgo lei grandfather asd 1872 dbd dss asd dbd dds hell asd eBd Sss aSa ddb ac dss grandfather A1a Tkg Ya1 A1a Tkg Ya1 C1u Zeg Tkg Bie Zeg Not O1u Tkg A1a A1a 610 615 620 Sss Sed 1920 garden abs grandfather sbs dBe hell aad abs dds dss das abd abd ace Zeg Zeg C1p Not A1a Bie Ya1 Zeg Bz Not C1u Ya1 Azr Month MeB Tkg 625 630 635 640 grandfather ddd 1968 grandfather ASD dds dsa ax sds hell grandfather grandfather asa csd hell dss asd A1a C1u A1a MeB C1u A1a 11th Agd Zeg A1a A1a Tkg Bie Zeg A1a Tkg 645 650 655 grandfather yes 1998 garden Bass aah eBd Sas sds dss Bda A1a Azr C1p Tug Azp Bie Tug Agd A1a ★ 660 665

<210> 7 <211> 665 <212> Rkt <213> Spillpotasis sp. ATCC53159 <220> <221> REAR <222> (0) ... (0) <223> ricky <400> 7 MeS Rgo Azr 11e Not Wa1 Luz Azp b1p Tkg s1i Ley Azp A1a A1a Ne one 5 10 fifteen

- 39 015170

A1a A1a A1a bc C1u 61u 01i Tht Not LUy A1a A1a C1u Ya1 Tug twenty 25 thirty Tht Azr Bie Tht Ya1 Me Tht Bz Tht Pb Tht Zeg Me Ya1 Tht Not 35 40 45 C1 and Zeg Bie Azr Zeg Zeg Azp Rgo Ya1 Azp Not C1p Bz Bie Ya1 Not fifty 55 60 61y Azp Zeg Zeg Azp Ya1 Tht Ya1 Bz Azp Me Ya1 A1a A1a Tht Azr 65 70 75 80 Tug Bz Rgo A1a Azr Azr Tgr Azp Agd Bie Azp Tht Not C1p C1u Zeg 85 90 95 A1a Azp Not Ya1 Bie Azr C1U Ya1 Agd Pb Zeg C1U 61y Tht C1u Azr one hundred 105 but Rgo Zeg Bie Zeg Bz C1u A1a C1u Bie Pb Ya1 Agd Azp Zeg Tht Zeg 115 120 125 Ya1 Tht Me C1p Azp Zeg Zeg Not Azr H1z Pb 61y Bie 61y Bie C1 and 130 135 140 A1a Tug Azp Ya1 Azr C1u MeC Ya1 Ya1 C1p Azp Zeg Zeg Pb H13 Azr 145 150 155 160 Azp Agd Agd Azr H1z Tht Azp Pb Tht C1 and Me1: Azp Azp Bie Ya1 Not 165 170 175 Azr C1u Azp Zeg Pb Tht Azp Bie Pb Rgo Ya1 61y Tht 61and H1z Rgo 180 185 190 Azr Aha Not C1p Pb Pb Tht A1a C1u Bz Ya1 Bz C1u Azp Tht Azp 195 200 205 Not Tht Not Zeg Azp Azp Ya1 Not Me 61p C1u A1a 61y Zeg C1u A1a 210 215 220 61p C1U Not Pb Me Azp Azr C1 and A1a 61y Azp Bie Rgo Tug Ya1 Azp 225 230 235 240 Ya1 Azp Not Bz Azp Azp Bie Not Tug Bie Azp C1u Tug Tug Nhz 61y 245 250 255 Not Azp Ya1 Ya1 Azp C1u Ya1 Azp Ya1 Azp 11th O1i Zeg Azp Zeg Ya1 260 265 270 Not Zeg C1p Ya1 Azr C1u Tht Zeg Pb Tgr Not Agd Bie Azr Bz Tht 275 280 285 Azp 61y A1a TNG Not Bz Azp Azp Ya1 A1a Azr Bie Not Tht Ya1 Tht 290 295 300 Zeg Zeg Zeg Zeg Azp Not Ya1 C1p Tht 61y Azp Agd Tht Bie Tht Zeg 305 310 315 320 Azr Zeg A1a Tht Not Agd Bz Not Tug C1u Bie Azp Azr C1u A1a Tht 325 330 335 A1a Agd Bie Zeg Azr Bie Not Ya1 Rgo 01y Ya1 O1U Tug 61p Rgo Rgo 340 345 350 Ya1 Zeg Zeg Aha A1a A1a A1a <31p Ya1 Tht Tht C1 and Bie Zeg Tht A1a 355 360 365 Bz A1a A1a Azp Rgo Zeg Bie Bie Bie Azr Bie Zeg Pb Zeg Azp Zeg 370 375 380 e1u Ya1 Ya1 Azr Bie Zeg ΗΪ3 Tgr Azp Tht C1u b1p Tht Tht Bz A1a 385 390 395 400 Ya1 Azr Ya1 Zeg A1a Ya1 Ya1 C1u Zeg A1a Pb H1z Ya1 Zeg Tht C1u 405 410 415 Tht (31u Ya1 <31and Bie Azp Agd Zeg Tug Zeg Agd O1p Not Tug A1a Bie 420 425 430 Zeg A1a Pb Tht Bie Zeg Pb Azr Bie Bz Agd Azr Zeg A1a Tht A1a 435 440 445 Tht A1a C1u C1p Not Bie C1U Not Pb C1p Zeg Tgr Zeg Ya1 Zeg Bie 450 455 460 01p A1a Azp C1u O1i Bie Zeg Pb Tht MeC Agd Azp A1a A1a C1u Ya1

- 40 015170

465 470 475 480 Zeg C1p Bj Meb Ya1 Bj Zeg 61y A1a Bz Bie Bie Azr A1a A1a Bj 485 490 495 H1z Bz Not A1a Bie Bj Tug Azr Zeg Bj Agd Bz YOU A1a 11th Bie 500 505 510 Tug Ya1 Azr 61y Meb 61 p Agd 61y Bj A1a Bj Meb TNG 61y Bj YOU 515 520 525 Agd Rgo A1a 61and Zeg Tgr 61y Bie Tug Ya1 61y Zeg Rgo Ръе Zeg Bj 530 535 540 A1a Ръе Zeg 61y YOU Ya1 C1u Azr Not 61and Not Agd Azr 61y A1a Not 545 550 555 560 Zeg A1a A1a 61p Ya1 61p A1a Bie Ya1 Bj A1a Zeg Zeg A1a Zeg A1a 565 570 575 A1a A1a Bj Ya1 Bz Azr Zeg Bie Ya1 Bj 61 y A1a A1a A1a C1p A1a 580 585 590 A1a A1a Bie Bie A1a 61y A1a C1u A1a A1a Zeg Bj A1a Bj Rgo Bie 595 600 605 A1a Bj Ya1 A1a Bj Ya1 01y Zeg Bj Bie Zeg Not 61 y Tht A1a A1a 610 615 620 Zeg Zeg 61p Not A1a Bie Ya1 Zeg Bz 11th 61y Ya1 Azr Meb Meb Bj 625 630 635 640 A1a C1u A1a Meb 61y A1a Not Agd Zeg A1a A1a Bj Bie Zeg A1a Bj 645 650 655 A1a Azr 61p Tug Azp Bie Tug Agd A1a

660 665 <210> 8 <211> 939 <212> ϋΝΑ <213> ATCC53159 <220>

<221> POPs <222> (1) ... (939) <223> s1pz <2 <400> 8

abd garden abd 48 sbs Bie csa asd sss dub dbe hell Zeg 10 aba Not sbs dbd dbe dsb bbe Ръе Rgo 5 Tht Meb one 61p Meb Rgo AZR Ya1 Bie Ya1 Ya1 A1a fifteen aas troubles ace dad women abs yeah das bda ebd sds dds abs dss yeah dda 96 Azp Zeg Bj C1 and Tug Not 61and Azr Suz Bie Agd 61 y Not 61and C1u twenty 25 thirty

grandfather dds 144 aad ace sss sas yeah dbb ebd ebd abs das aab dds 61y das ddd Not azr Azp 45 A1a 61y Bz 35 Bj Rgo Н1з 61и Уа1 40 Bie Bie Azr 61y food ace yeah grandfather ebd dbe sdd garden sdd bbe sas sas dbe sds abs dbb 192 Agd Bj 61and A1a Bie Ya1 Agd 61p Agd Pb ΗΪ3 ΗΪ3 Ya1 Agd Not Ya1 fifty 55 60

- 41 015170

sss 240 Rgo € 5 hell give dds C1u aab Azp ab Not 70 dds C1u Bc Pb ddd SHU dss A1a dds 01 y 75 aab Azp aab Azp sds Agd cd Bie dsa A1a 80 Zeg SHi grandfather garden dcb dss dds ssd cbc cd cd cbc ds aas sss yes dss ab 288 A1a C1l A1a A1a C1u Rgo Bie Bie Bie Bie Ya1 Azp Rgo Azr A1a Not 85 90 95 sss garden sss dds dsa abc yes garden Bd dc ace B dss aah garden sa 336 Rgo C1p Rgo C1u A1a Not Azr C1p Bie Wah Tht Pb A1 a Bz SHP ΗΪ3 one hundred 105 110 sss dad grandfather grandfather dsa SDD dds dds ed Bie Bac Bcd sss hell dds yes 384 Rgo C1 and A1a A1a A1a Tgr SHU C1u Agd Zeg Tug Zeg Rgo Zeg SHU Azr 115 120 125 ba yeah sss dsa aab Bc ab Bie cd ssd asd sss dss das B cd 432 Bie C1 and Rgo A1a Azp Pb Me Zeg Bie Rgo Tht Rgo A1a Azr Pb Bie 130 135 140 asd grandfather ab Bc aas grandfather ed grandfather ba sds hell dds ddd cd saa yeah 480 Tht A1a Not Pb AZP A1a Agd Aha Bie Agd Zeg C1u SHU Bie SHP C1 and 1 L E 1 C L 1 C C hee IE l and dds grandfather ace ace sss <3 <3 ^ grandfather des dad grandfather Bd aab dds dds Bc ab 528 C1u A1a Tht Tht Rgo C1u A1a Ya1 SHi Wah Bie Azp C1u C1u Pb Me 165 170 175 ab dha sds ace yes des edd garden grandfather abc dds dds b das dad hell 576 Me Ya1 Agd Tht Azr Ya1 Tgr SHP A1a Not SHU C1u Pb Azr C1 and Zeg 180 185 190 b B bb Ba Bcd yeah dad abc yes cbc Bc garden food abc sds asd 624 Pb Pb Bie Tug Zeg C1 and SHi Not Azr Bie Pb C1p Agd Not Agd Tht 195 200 205 bld ddd sas aad grandfather cbc dc das sss Bcd dc aah grandfather dha sas aab 672 Bie C1u ΗΪ3 Bz Ya1 Bie Wah Azr Rgo Zeg Ya1 Bz Ya1 Ya1 Ηίβ Azp 210 215 220 asd ddd hell dds garden Bcd ab Bie garden aas sds cd asd Ba cbc asd 720 Tht C1u Zeg C1u C1p Zeg Me Zeg 610 Azp Agd Bie Me Tug Bie Tht 225 230 235 240 ace ddd sds ab sas Ba grandfather food aad sa b dds dsa cbc dds ace 7 68

- 42 015170

TNG C1u Agd MeE H1z 245 Tug A1a Agd Luz H13 250 Pb 61y A1a lei C1u 255 Tht CEE dss ace ddd Food grandfather CEE Unit eat aec dss dss aah Eae asd Food 816 Bie A1a Tht C1u Suz A1a Bie Tgr Bie Not A1a A1a Bz Tug Tht Bie 260 265 270 des ddd grandfather dsa cEC Unit sds eat Eating ssd edd asd dde asd food Eae 864 Ya1 61y A1a A1a Bie Tgr Agd Bie Zeg Rgo Agd Tht C1u Tht Agd Tug 275 280 285 aah dad eat hell aas ddd Unit cde dss yeah Eee hell aaE seE dde Oh yeah 912 Bz 61and Bie Zeg Azp 31 y Tgr Agd A1a Ya1 Pb Zeg Azp Rgo C1u Agd 290 295 300 ^ dd Unit hell dde EAE ssd cde eating Yeah 939 Tgr Tgr Zeg C1u Tug Rgo Agd Agd *

305 310 <210> 9 <211> 312 <212> RKT <213> ATCC53159 <220>

<221> ΡΕΡΤΙΟΕ <222> (0) ... (0) <223> Risayhu Ghatposu! Egapz Egase IV <400> 9

MeE one C1p MeE Yee Rgo Th2 5 Rgo Azr Wa1 Zeg 10 Not Bie Ya1 Ya1 A1a fifteen Pb Azp Zeg Tht 31and Tug 11th 31and Azr Suz Bie Agd 31u Not A1a C1 and 31u twenty 25 thirty A1a 61y Bz Tht Rgo H1z C1 and Ya1 Bie Bie Not Azr Azp 31u Azr C1U 35 40 45 Agd Tht C1 and A1a Bie Ya1 Agd 31 p Agd Pb H1z H1z Ya1 Agd Not Ya1 fifty 55 60 Rgo Zeg C1 and C1u Azp Not 61y Pb 31u A1a 61y Azp Azp Agd Bie A1a 65 70 75 80 A1a 61p A1a A1a 31u Rgo Bie Bie Bie Bie Ya1 Azp Rgo Azr A1a Not 85 90 95 Rgo C1p Rgo C1u A1a Not Azr C1p Bie Ya1 Tht Pb A1a Bz C1p ΗΪ3 one hundred 105 110 Rgo C1 and A1a A1a A1a Tgr C1u 31u Agd Zeg Tug Zeg Rgo Zeg 31u Azr 115 120 125 Bie 61and Rgo A1a Azp Pb MeE Zeg Bie Rgo Tht Rgo A1a Azr Pb Bie 130 135 140 Tht A1a Not Pb Azp A1a Agd A1a Bie Agd Zeg 31u C1u Bie C1p 61and 145 150 155 160 b1u A1a Tht Tht Rgo 61y A1a νβΐ C1 and Ya1 Bie Azp C1u 31u Pb MeE 165 170 175

- 43 015170

MeC Va1 Arg Thb Azr Bb Sue Sue Rye Azr B90 SI Zeg 180 185 Pb Pb Bie Tug Zeg SI SI 1b Azr Bie Pb Bn Agd Bb Agd Tht 195 200 205 Bie Sue Nhz Bz Yb Bie Yb Azr Rgo Zeg Yb Bz Yb Yb NHK Azp 210 215 220 Tht Sue Zeg Sue Bn Zeg MeC Zeg Bn Azp Agd Bie MeC Tug Bie Tht 225 230 235 240 TNG C1u Agd MeC Hb Tug Aba Agd Bz Hb Pb Sue Aba Bie Sue Tht 245 250 255 Bie Aba TNG Sue Suz Aba Bie Tgr Bie Bb Aba Aba Bz Tug Tht Bie 260 265 270 Yb Sue Aba Aba Bie Tgr Agd Bie Zeg Rgo Agd Tht Sue Tht Agd Tug 275 280 285 Bz SI Bie Zeg Azp Sue Tgr Agd Aba Yb Pb Zeg Azp Rgo Sue Agd 290 295 300 Tgr Tgr Zeg Sue Tug Rgo Agd Agd

305 310 <210> 10 <2Ы> 972 <212> ΟΝΑ <213> ATCC53159 <220>

<22 b> POPs <222> (1) ... (972) <223> drz <400> 10

ASD SSD sas Hb Vds Suz saa Bn 5 sds sas sds dda Sds ssds sss des food Agd aaa Bb B5 ddE Sue 48 MeC B Bie Suz B Rgo Agd Tug Suz Sue Rgo Yb SSS hell hell hell SSD Vds sdS grandfather saa SSc sdS dss dds ddd dad des 96 Zeg Zeg Asd Zeg Bie Suz Agd Aba Bn Pb Agd Aba Sue Sue SI Yb twenty 25 thirty SSc ASD aad aah SSD Sas csd DSS asd grandfather dsd DSE grandfather dss grandfather ssd 144 Pb MeC Bz Bz Bie Tug Bie Yb Tht Aba Yb Aba Aba Aba Aba Bie 35 40 45 dss dss Sss dda SDS dds dds aad dds dds aad cEC das aad ddd garden B92 Aba Yb Zeg Sue Suz Sue Sue Bz Sue Sue Bz Bie Azr Bz Sue Bn fifty 55 60 dsd des dss hell dss yes dds yeah yeah ACC ace des EEC dad eat aaE 240 Yb Yb Aba Zeg Yb Azr Sue SI SI Bb Tht Yb Pb SI Bie Azp 65 70 75 80 dss yeah csd garden dss Sss garden dSa sss ssd ddd ace Dan sds aad eat

288

- 44 015170

A1a SI Bie Cbn A1a 85 Zeg Bn Yb Rgo Рgo СЬу 90 Tkg Azr Agd Bz 95 Bie dss dad garden eat grandfather skd garden sds aec aec dad sds aad aec cEC dss 336 Aba SI Bn Bie A1a Bie Bn Agd Bb Not SI Agd Bz Bb Bie Aba one hundred 105 110 aad dke dss sds dad garden aad cd das aad aed sce dss EEC skd ax 384 Bz Yb A1a Agd SI Bn Bz Bie Azr Bz Tkg Rgo A1a Rke Bie Bb 115 120 125 garden dad eating edd dss das dad cd aec cEC ace dss akd skd eating das 432 Bn SI Agd Agd Aba Azr SI Bie Not Bie Tkg Aba MeE Bie Agd Azr 130 135 140 aad ax dss dds dds ax dss garden eat ace yes dss dad ax dad aah 480 Bz Not A1a C1u Sue Bb Aba Bn Rgo tkg Azr Aba SI B1e SI Bz 145 150 155 160 as garden dss grandfather sack eat dad edd EEC grandfather garden sds aad aec Eae grandfather 528 Tug Bn A1a Aba Hb Rgo SI Agd Rke Aba C1p Agd Bz Not Tug A1a 165 170 175 ax yes garden dke dke Bb dcb eat eat hell sneaker dss dsa aad cEC aad 576 Bb Azr Bn Yb Yb Rke Aba Rgo Rgo Zeg Zeg Aba Aba Bz Bie Bz 180 185 190 saa Bb grandfather eat skd aad aed eat das garden cea ace dss aad cEC Eating 624 Bn Rke Aba Rgo Bie Bz Tkg Bie Azr Bn Bie Tkg A1a Bz Bie Zeg 195 200 205 grandfather das aaE des garden Bb caustic sds grandfather sed Eating garden aec das ace DSE 672 Aba Azr Azp Yb Bn Rke Agd Agd Aba Rgo Zeg Bn Bb Azr Tkg Aba 210 215 220 grandfather skd sed sed yeah abc Dec dss aad aec grandfather Eating eat eat dsa garden 720 A1a Bie Rgo Rgo SI Not Aba Aba Bz Bb Aba Zeg Bie Rgo Aba Bn 225 230 235 240 dad akd EEC aec skd eat ace garden garden dda eat ace grandfather aaE ax ax 768 SI Mek Rke Not Bie Rgo Tht Bn Bn Sue Bie Tkg Aba Azp Not Bb 245 250 255 aed sneaker ace aed grandfather eat eat dkd eat dss das garden grandfather sds dad ax 816 Tht Zeg Tkg Tkg Yb Bie Rgo Yb Rgo Aba Azr Bn Aba Agd SI Not 260 265 270

- 45 015170

grandfather cec ad ddd C1u sEd sde ace dad sds EEC DDS AAD grandfather dse das dsa A1a 864 A1a lei Zeg 275 Bie Agd Thr 61and 280 Agd Pb C1u Bz A1a 285 A1a Azr garden cEC aas das sds eat aad aad grandfather edd yeah ace grandfather aah EAE garden 912 C1p Bie Azp Azr Agd Bie Bz Bz A1a Agd C1 and Tht Ya1 Bz Tug C1p 290 295 300 dss dds Eas hell dsa sed ssd garden c £ E eating dds hell dds dsa asd ssd 960 A1a C1u Tug Zeg A1a Rgo Rgo 61p Bie Agd C1u Zeg C1u A1a Tht Rgo 305 310 315 320

grandfather ddd aas food

972

A1a C1u Azp * <210> 11 <211> 323 <212> RKT <213> ATCC53159 <220>

<221> ΡΕΡΤΙϋΕ <222> (0) ... (0) <223> ipkip <400> 11

MeE Bie Nhz Suz C1p Agd Tug Suz 61y Suz Rgo Rgo Ya1 Agd Not 61y one 5 10 fifteen Zeg Zeg Agd Zeg Bie Suz Agd A1a C1p Pb Agd A1a C1u C1u 61and Wah! twenty 25 thirty Pb MeE Bz Bz Bie Tug Bie Ya1 YOU A1a Ya1 A1a A1a A1a A1a Bie 35 40 45 A1a Ya1 Zeg b1u Suz 61y C1u Bz C1u C1u Bz Bie Azr Bz 01 y C1p fifty 55 60 Ya1 Ya1 A1a Zeg Ya1 Azr C1u 61and C1 and Not Tht Ya1 Pb C1 and Bie Azp 65 70 75 80 7k Ί 01 and T y-M- γ ι Αία. V- one Rho g? l ,, ty ν- T., 7H Yo GKH <3 is and CH7XX1 msx 47 X 1 1 ν a x £ X ν X 1 4-L. Azr Agd “G 85 90 95 A1a C1 and C1p Bie A1a Bie C1p Agd Not Not C1 and Agd Bz Not Bie A1a one hundred 105 BUT Bz Ya1 A1a Agd C1 and C1p Bz Bie Azr Bz Tht Rgo A1a Pb Bie Not 115 120 125 C1p C1 and Agd Agd A1a Azr 61and Bie Not Bie Tht A1a MeE Bie Agd Azr 130 135 14 0 Bz Not A1a C1u C1u Not A1a 61p Rgo Tht Azr A1a C1 and Not C1 and Bz 145 150 155 160 Tug C1p A1a A1a Nhz Rgo 61and Agd Pb A1a C1p Agd Bz Not Tug A1a 165 170 175 G1e Azr 61p Ya1 Ya1 Pb A1a Rgo Rgo Zeg Zeg A1a A1a Bz Bie Bz 180 185 190

- 46 015170

61p RNe A1a Rgo Bie Bz TNG Bie 200 Azr 61p Bie TNG A1a 205 Bz Bie Zeg 195 A1a Azr Azp Ya1 C1p RNe Agd Agd A1a Rgo Zeg C1p Not Azr TNG A1a 210 215 220 A1a Bie Rgo Rgo 61and 11th A1a A1a Bz 11th A1a Zeg Bie Rgo A1a C1p 225 230 235 240 C1 and Mek RNe Not Bie Rgo TNG 61p 61p 61y Bie TNG A1a Azp Not Not 245 250 255 TNG Zeg TNG TNG Ya1 Bie Rgo Ya1 Rgo A1a Azr 61p A1a Agd 61and 11th 260 265 270 A1a Bie Zeg C1u Bie Agd TNG 61and Agd RNe C1u Bz A1a A1a Azr A1a 275 280 285 C1p Bie AZP Azr Agd Bie Bz Bz A1a Agd 61and TNG Ya1 Bz Tug C1p 290 295 300 A1a 6.1y Tug Zeg A1a Rgo Rgo 61p Bie Agd C1u Zeg 61y A1a TNG Rgo 305 310 315 320 A1a 61y Azp

<210> 12 <2Η> 1047 <212> ΩΝΑ <213> Sp. ATCC53159 <220>

<221> SEZ <222> (1) ... (1047) <223> <ZrzK

<400> 12 akd 48 Mek one dsa daa grandfather aas grandfather dka dak dda aad dss kss aad ssd skd aaa Rgo ley bz fifteen A1a 61 and A1a Azp 5 A1a Wa1 Azr 61y Bz 10 A1a Zeg Bz akd kde CEE dsa grandfather Esd dds dds dds sack SCS edd saa ax SCS duck 96 Mek Suz Bie A1a A1a Zeg 61y 61y 61y ΗΪ3 Bie Agd 61p 11th Bie Azr twenty 25 thirty skd yeah Esd dkd cdd sds yeah sas duck EAE EEC EEC dkk ask yeah duck 144 Bie 61and Zeg Ya1 Tgr Agd C1 and H1z Azr Tug RNe Pb Ya1 TNG 61and Azr 35 40 45 ace grandfather cEC dds edd hell Skk dss yeah aah sack sss dke yeah skd dkd 192 TNG A1a Bie 61y Agd Zeg Bie A1a 61and Bz ΗΪ3 Rgo Ya1 61and Bie Ya1 fifty 55 60 dad sas EAE grandfather SCS dds garden dss aad skd dds sack sss ccd sds akd 240 61 and H1z Tug A1a Bie 61y 61p A1a Bz Bie 61y Naz Rgo Bie Agd Mek 65 70 75 80 skd dds dds dsa Unit sds aas skd sds garden hell skk Esd ax skd sds 288

- 47 015170

Ley Sue Sue Aba Tgr agd azp bey agd bn Zeg Bie Zeg S Bie 95 Agd 85 90 edd sas aad ssd duck dkd dkd acc css ace dds grandfather dds dsa dke as 336 Agd Hb Bz Rgo Azr Yb Yb S Zeg Tht Sue Aba Sue Aba Yb Tug one hundred 105 110 cx ace grandfather skd SCS dss aah skd sneaker dds dss aad cx dke sack ax 384 Pb Tht Aba Bie Bie Aba Bz Bie Zeg Sue Aba Bz Pb Yb Hb S 115 120 125 yeah hell cx grandfather sds cx das sas sed cake dss cx dds aad akd dkd 432 SI Zeg Pb Aba Agd Pb Azr Hb Rgo Zeg Aba Pb Sue Bz Mek Yb 130 135 140 aad dds ax grandfather asd dkd asd ax dke garden sneaker grandfather grandfather skd aah yeah 480 Bz Sue S Aba Tht Yb Tht S Yb Bn Zeg Aba Aba Bie Bz SI 145 150 155 160 ace cdd sec duck dss dad skd cx duck ssd cx sds skd SCS duck asa 528 Tht Tgr Rgo Azr Aba SI Bie Pb Azr Rgo Pb Agd Bie Bie Azr Tht 165 170 175 ssd sds ssd sss aad garden grandfather ska ax cx grandfather asd dke dds dss ace 576 Rgo Agd Rgo Rgo Bz Bn Aba Bie S Pb A1a Tht Yb Sue Aba YOU 180 185 190 skd sss cx sed edd skd dkd garden dsa dd SCS das skd aad sds dss 624 Bie Rgo Pb Rgo Agd Bie Yb Bn Aba Yb Bie Azr Bie Bz Agd Aba 195 200 205 dds ddd skd ssd dds aad skd ax skd saa as dds das garden das skd 672 Sue Sue Bie Rgo Sue Bz Bie S Bie Bn Tug 61y Azr Bn Azr Bie 210 215 220 sss duck sss dds ax sss das dke dad ax eating caustic ace ax ssd cx 720 Rgo Azr Rgo Sue S Rgo Azr Yb SI S Agd Agd Tht Bb Rgo Pb 225 230 235 240 das duck skd garden skd skd skd sds duck grandfather duck akd dkd aka kde sas 768 Azr Azr Bie Bn Bie Bie Bie Agd Azr Aba Azr Mek Yb S Suz Hb 245 250 255 dds dds ace dda sneaker skd dke asd grandfather skd eating dss dds kde edd dke 816 Sue Sue Tht Sue Zeg Bie Yb Tht Aba Bie Agd Ab a Sue Suz Agd Yb

260 265 270

- 48 015170

des ds ese ssd Rgo sds Agd sds Agd sas yes ced dds dad sae Nbz EAE Tug 285 das Azr yes Azr sas Nbz 864 Oub Aba Pb 275 nbz Azr 280 Bie SBU Sbi garden yeah dad aec dss garden ace EEC dss das sdd dds eat cEC garden grandfather 912 Sbp Sbi Sbi Not Aba Sbp Tht Pb Aba Azr Agd SBU Bie Bie Sbp Aba 290 295 300 grandfather sds das dad sds garden cEC dds dss DSE dd yeah grandfather dss aad dsa 960 OUB Agd Azr Sbi Agd Sbp Bie SBU Aba Aba OUB Sbi Aba Aba Bz Aba 305 310 315 320 ace dad ssd garden eat grandfather ace ace das sas asd dss cEC grandfather grandfather sdd

1008

Tht Sbi Rgo Sbp Bie Aba Tht Tht Azr Nbz Tht Aba Ley Aba Aba Agd 325 330 335 eat sds garden eat eat grandfather garden Unit hell dss aad food Food 1047 Bie Agd Sbp Bie Bie Aba Sbp Tgr Zeg Aba Bz Agd . * 340 345

<210> 13 <211> 348 <212> RKT <213>

ATCC53159 <220>

<221> PERTURBE <222> (0) ... (0) <223> L eEa-1,4-dbisigopod

<400> 13 MeE A1a Sbi Aba Azp Aba OUB Azr SBU love Aba Zeg Bz Rgo Bie Bz one 5 10 fifteen MeE Suz Bie Aba Aba Zeg SBU SBU SBU Nbz Bie Agd Sbp Not Bie Azr twenty 25 thirty Bie Sbi Zeg OUB Tgr Agd Sbi Nbz Azr Tug Pb Pb OUB Tht Sbi Azr 35 40 45 Tht Aba Bie SBU Agd Zeg Bie Aba Sbi Bz Nbz Rgo OUB Sbi Bie OUB fifty 55 60 Sbi Nbz Tug Aba Bie SBU Sbp Aba Bz Bie SBU Nbz Rgo Bie Agd MeE 65 70 75 80 Bie SBU SBU Aba Tgr Agd Azp Bie Agd Sbp Zeg Bie Zeg Not Bie Agd 85 90 95 Agd Nbz Bz Rgo Azr OUB OUB 11th Zeg Tht SBU Aba SBU Aba OUB Tug one hundred 105 110 Pb Tht Aba Bie Bie Aba Bz Bie Zeg SBU Aba Bz Pb OUB Nbz Not 115 120 125 Obi Zeg Pb Aba Agd Pb Azr Nbz Rgo Zeg Aba Pb SBU Bz MeE OUB 130 135 140 Bz SBU 11th Aba Tht OUB Tht Not OUB Sbp Zeg Aba Aba Bie Bz Sbi

- 49 015170

145 150 155 160 Tkg Tgr Rgo Azr A1a C1 and Bie Rke Azr Rgo Rke Agd Bie Bie Azr Tkg 165 170 175 Rgo Agd Rgo Rgo Bz 61p A1a Bie 11th Rke A1a Tkg Ya1 C1u A1a Tkg 180 185 190 Bie Rgo Rke Rgo Agd Bie Ya1 61p A1a Ya1 Bie Azr Bie Bz Agd A1a 195 200 205 61y 61y Bie Rgo 61y Bz Bie Not Bie 61p Tug 61y Azr 61p Azr Bie 210 215 220 Rgo Azr Rgo C1u Not Rgo Azr Ya1 61and 11th Agd Agd Tkg Not Rgo Rke 225 230 235 240 Azr Azr Bie 61p Bie Bie Bie Agd Azr A1a Azr Mek Ya1 Not Suz Ηί3 245 250 255 61y 61y Tkg C1u Zeg Bie Ya1 Tkg A1a Bie Agd A1a 61y Suz Agd Ya1 260 265 270 Ya1 A1a Rke Rgo Agd Agd H1z Azr Bie 61 y 61and ΗΪ3 Tug Azr Azr Bottom 275 280 285 Θΐη 61and 61and 11th A1a C1p Tkg Rke A1a Azr Agd 61y Bie Bie 61 η A1a 290 295 300 Ya1 Agd Azr C1 and Agd 61p Bie 61y A1a A1a Ya1 61and A1a A1a Bz A1a 305 310 315 320 Tkg 61and Rgo C1p Bie A1a Tkg Tkg Azr Nhz Tkg A1a Bie A1a A1a Agd 325 330 335 Bie Agd 61p Bie Bie A1a C1p Tgr Zeg A1a Bz Agd 340 345

<210> 14 <211> 867 <212> ϋΝΑ <213> ATCC53159 <220>

<221> C3 <222> (1). . . (867) <223> <1p3 <400> 14

abd 48 Month one hell Zeg asd sss sdd Agd 5 ACC 11th Ade dbs dss abs Not 10 ssd Rgo sas Naz CaS Tug aah Azp yes Azr fifteen ssd Rgo Tkg Rgo Zeg Ya1 Ya1 saa Sss Bsd sdd ssc Bds eBd yes grandfather eBd dad sdd garden asd ACC dds 96 61p Zeg Bie Agd Bie Suz Bie Azr A1a Bie 61and Agd 61p Tkg Not C1u twenty 25 thirty sds das grandfather SSc dad abs ACC dss dds das aah aaS Ssd sss SDS ddd 144 Agd Azr A1a Rke C1 and X1e Not Ya1 C1u Azr Azp Azp Zeg Rgo Suz C1U 35 40 45 ssc grandfather grandfather dbd dad grandfather grandfather dss dss dda sdS grandfather sdd ACC dbd ace 192 Bie A1a A1 a Ya1 C1 and A1a A1a Ya1 A1a 61y Agd A1a Agd Not Ya1 Tkg fifty 55 60

- 50 015170

ACC sed 240 11th lei 65 yeah 01and aad Bz ddd ddd dds sss ded sds aas ddd grandfather dsa dss dsa A1a 80 C1u A1a 70 B1u Rgo A1a Agd Azp 75 C1u A1a A1a A1a grandfather sdS dds dad aes cbc dss eee ass das hell das eating grandfather grandfather dad 288 A1a Agd C1u C1 and 11th Bie A.1a Pb Tht Azr Zeg Azr Suz Ya1 Ya1 C1 and 85 90 95 sss dds edd sed grandfather dds dds asd ass add des grandfather sse dds eating eee 336 Rgo C1u Tgr Bie A1a B1u C1u Tht Tht Agd Ya1 A1a Rgo 61y Agd Pb one hundred 105 110 ax dds ddd sas ad eae grandfather sds aad sss yeah ddd ssd ssd aas dds 384 11th C1u C1u H1z Mee Tug Ya1 Agd Bz Rgo C1 and 61y Rgo Rgo Azp 61y 115 120 125 dss dad grandfather sed dad aed grandfather sed grandfather eee das aah yeah dds eae grandfather 432 A1a C1 and A1a Bie C1 and Mee A1a Bie A1a Pb Azr Azp C1 and C1u Tug Ya1 130 135 140 sdd sds ass garden eee asd des ass dsa aas sed eee grandfather aed sds dss 480 Agd Agd Tht B1p Pb Tht Ya1 Tht A1a Azp Bie Pb Ya1 Mee Agd A1a 1 le 1 C L 1 SS ± and ν give eee yeah sdd des dds dds eee sds more dds grandfather ess dad give sed 528 Azr Pb B1i Agd Ya1 C1u C1u Pb Agd Ya1 C1u Ya1 Zeg C1 and AZR Bie 165 170 175 yeah edd Vds sas sdd grandfather aes dss hell dds ses ass aes aas eae dsa 576 C1 and Tgr Suz Nhz Agd A1a 11th A1a Zeg C1u Bie Tht 11th Azp tug A1a 180 185 190 ssd give dsa eat grandfather dds sas ssd sss sdd sss das edd eat dss sed 624 Rgo d yag " A1a Zeg Ya1 01y Rgo Rgo Dgggg Rgo Dyap Tgr Zeg A1a Bie ' FROM • from 195 200 205 sed grandfather aad asd sdd sds a £ s garden sds yeah ses eae sed eee aas aes 672 Bie Ya1 Bz Tht Agd Agd 11th C1p Agd C1 and Bie Tug Bie Pb Azp 11th 210 215 220 dad sdd ssd aad dds add sed sds edd sed des eating ess grandfather grandfather saa 720 61and Agd Rgo Bz C1u Agd Bie Agd Tgr Bie Ya1 Agd Zeg Ya1 A1a C1P 225 230 235 240 ssd grandfather aed aes ssa garden das grandfather dss aad aes sed sds asa ssd dde

768

- 51 015170

Rgo A1a Me Not Rgo <31p Azr Wa1 A1a 245 Bz 250 11th Ley Agde Tht Rgo 255 C1u ace 816 aad dds grandfather sds Cbc dcb grandfather dc ace asd cd dc edd cd edd Tht Bz C1u A1a 260 Agd Bie A1a A1a Ya1 265 Tht Tht Bie Ya1 Agd 270 Bie Agd cd 864 Dd sds dds dds dss dds Bd Bd garden Bd cbc dds sds das abc Bie Tgr Agd 275 C1u C1u A1a C1u Bie 280 Bie 61p Bie Bie 61y 285 Agd Azr Not Bda 867 to

<210> fifteen <211> 288 <212> Rkt <213> Spillpotasis sp. ATCC53159 <220> <221> REAR <222> (0). . . (0) <223> d1isosu1 bacillus segase III

<400> 15

Me Zeg Tht Rgo Agd Not Zeg Ya1 Ya1 Not Rgo H1z Tug Azp Azr Rgo one 5 10 fifteen 61p Zeg Bie Agd Bie Suz Bie Azr A1a Bie C1 and Agd C1p Tht Not C1u twenty 25 thirty Agd Azr A1a Pb C1 and Not Not Ya1 C1u Azr Azp Azp Zeg Rgo Suz C1u 35 40 45 Bie A1a A1a Ya1 C1 and A1a A1a Ya1 A1a C1u Agd A1a Agd Not Ya1 Tht fifty 55 60 11th Bie C1 and Bz 61y A1a C1u Rgo A1a Agd Azp 61y A1a A1a A1a A1a 65 70 75 80 A1a Agd C1u C1 and Not Bie A1a Pb YOU Azr Zeg Azr Suz Ya1 Ya1 C1 and 85 90 95 Rgo C1u Tgr Bie A1a C1u C1u Tht Tht Agd Ya1 A1a Rgo C1u Agd Pb one hundred 105 but Not C1u C1u H13 Me Tug Ya1 Agd Bz Rgo E1i C1u Rgo Rgo Azp C1u 115 120 125 A1a C1 and A1a Bie E1i Me A1a Bie A1a Pb Azr Azp E1i C1u Tug Ya1 130 135 140 Agd Agd Tht C1p Pb Tht Ya1 Tht A1a Azp Bie Pb Ya1 Me Agd A1a 145 150 155 160 Azr Pb C1 and Agd Ya1 C1u C1u Pb Agd Ya1 C1u Ya1 Zeg C1 and Azr Bie 165 170 175 C1 and Tgr Suz Nhz Agd A1a Not A1a Zeg C1u Bie Tht 11th Azp Tug A1a 180 185 190 Rgo Azr A1a Zeg Ya1 C1u H13 Rgo Rgo Agd Rgo Azr Tgr Zeg A1a Bie 195 200 205 Bie Ya1 Bz Tht Agd Agd Not C1p Agd C1 and Bie Tug Bie Pb Azp Not

- 52 015170

210 2X5 220 61and Agd Rgo Bz C1u Agd Bie Agd Tgr Bie Wah Agd Zeg Wah Aha SHP 225 230 235 240 Rgo A1a Me Hehe Rgo C1p Azr Wah Aha Bz Hehe Bie Agd Tht Rgo SHU 245 250 255 TNG Bz SHU Aha Agd Bie Aha Aha Wah Tht Tht Bie Wah Agd Bie Agd 260 265 270 Bie Tgr Agd SHU O1u Aha SHU Bie Bie SHP Bie Bie SHU Agd Azr 1He 275 280 285

<210> 16 <211> 1389 <212> ΡΝΑ <213> ATCC53159 <220>

<221> C3 <222> (1) ... (1389) <223> brzb <400> 16

grandfather 48 Wah ah X dha Wah dde SHU ess Zeg 5 grandfather A1a sed Bie ada ees edd ssd ees dds food food Agd Agd Rye Ley 10 Tgr Rgo Pb SHU Agd fifteen yeah yeah sse yeah yeah dad dds eae ees ssd sed ace grandfather asa grandfather grandfather 96 SHi SHi Rgo SHi SHi SHi SHU Tug Pb Rgo Bie Tht A1a Tht Wah Ya1 twenty 25 thirty ssd sae sds yes dss sae hell dds sd dde sds sed give ees ssa ace 144 Rgo ΗΪ3 Agd Azr Aha H13 Zeg SHU Agd 61U Agd Rgo Azr Pb Rgo Tht 35 40 45 ees eating dss ess grandfather sed das sds ssd sed give sds sds sds das dad

192

Rye Agd A1a Zeg A1a Ley Azr Agd Rgo Ley Azr Agd Agg Agd Azr 61i

55 60

sds 240 Agd 65 sds dad aes asd sds dss edd yeah grandfather sed grandfather ace ees ees asa Tht 80 Agd SHi 11th Pb A1a Ley Aha 75 Tht Agd 70 Aha Agd Pb Pb sss ace garden ssd grandfather dss give edd Bcd hell ees dss ddd sds cbc dds 288 Rgo Tht SHP Rgo Wah A1a Azr Agd Zeg Zeg Pb A1a SHU Agd Bie SHU

90 95

grandfather sed grandfather sds ba aes ess Bcd abc dad hell garden sds hell sae dc 336 Ya1 Bie A1a Agd Bie 11th Zeg Zeg 11th 61and Zeg C1p Agd Zeg NHC Ya1 one hundred 105 110

- 53 015170

grandfather cgs eae 384 Ba'e tug dds dad food dds aes dds aad ace eed sed ses sas Hb grandfather Yb Bie 125 Bie C1u SI Agd SI Bb 120 Sue Bz Tht Zeg 115 sed 432 ace Dan des dss eating yeah ess hell eae abc des hell eae grandfather ace Bie TNG 130 Azr Yb Aba Agd SI 135 Zeg Zeg Tug Not Yb 140 Zeg Tug Aba Tht eating 480 ddj grandfather aah grandfather aah eee hell give des eee eating dss grandfather sed yeah suz 145 C1u Aba Azp Aba Azp 150 Pb Zeg Azr Yb Pb 155 Agd Aba Yb Bie SI 160 das 528 grandfather eat sed sed eee sae eating dds grandfather grandfather sss aah dss dds dad Aar Yb Rgo Bie Bie 165 Pb Hb Agd Sue Yb 170 Aba Rgo Azp Aba Sue 175 SI grandfather 576 dad hell dds dds aah eat dss das eating cd eat asd dds hell eee Aba SI Zeg Sue 180 Sue Azp Bie Aba Azr 185 Agd Bie Rgo Tht Sue 190 Zeg RNe ddd 624 sss dds yeah sed dss das sed eating dss das aes asd dds asa eating eu Rgo C1u 195 C1 and Bie Aba Azr Bie 200 Suz Aba Azr Not Tht 205 Sue Tht Agd grandfather 672 sed aes ace ses das yeah eae give eating des hell give ess dss eee Yb Bie 210 Not Not Bie Azr SI 215 Tug Azr Agd Yb Zeg 220 Azr Zeg Aba RNe eating 720 garden garden des dss dad sed aes aad aah sed eat das eating eat grandfather Agd 225 Bn Bn Yb Aba SI 230 Bie Not Bz Azp Bie 235 Zeg Azr Agd Zeg Aba 240 eating 768 des garden with SD grandfather abc grandfather dds des dss eat aah sed garden dad cd Agd Yb 61 p Bie Yb 245 Not Aba 61y Yb Aba 250 Zeg Azp Bie Bn SI 255 Bie abc 816 ddj Us grandfather eat Sed aes eating eating aah des abc dds sed eat aed Bb C1u Tug Aba 260 Rgo Zeg Not Agd Agd 265 Azp Yb Not ST at Bie 270 Rgo Mee sss 864 edd cd dad yeah Sed dad grandfather garden dad aed aes grandfather ses dds yeah Rgo Agd Bie 275 SI SI Zeg SI Yb 280 Bn SI Mee Not Aba 285 Bie Sue SI ace 912 dss Sed dds more eating eee give sed das sed ace sas aed abc sas

- 54 015170

Bj A1a 290 Zeg SBU OUB Agd РЬ © Azr Rgo Azr Ley Тъг Нбз Meb Not Nbz 295 300 ebd sbs grandfather ebd ddd troubles STS women bbe grandfather sdd ebd ebd bda sas sub 960 Bie Bie Aba Bie C1u Zeg Rgo Tug Ръе A1a Agd Bie Bie Suz ΗΪ3 Nbz 305 310 315 320 bss grandfather ebd yeah dss ebd das garden dds sds sbs asd dbe das dss ddd

1008

Zeg Aba Ley Sbi Aba 325 Ley Azr Sbp SBU AGD LEE TG Uab Azr Aba SBU 330 335 sub ebd edb edb grandfather sbs das garden grandfather abs ebb dad abs dad dds eating 1056 Nbz Bie Agd Agd Aba Bie Azr Sbp Aba Not Bie Sbi Hbe Sbi SBU Agd 340 345 350 abd ssd ssd sds grandfather dbd abs dad abd sds aad bbe dbe dds dds sds

1104

Meb Rgo Rgo 355 Agd Aba OUB Not Sbi Meb 360 Agd Bz Ръе OUB 365 SBU SBU Agd bass 1152 dub ssa sbs dbe grandfather grandfather ebd dds dad dss troubles sds troubles grandfather dub Tug Azr 370 Rgo Bie OUB Aba Aba 375 Bie SBU 61and Aba Zeg 380 Agd Zeg Aba Azr dds bdd bbe hell dds saa dss dbd dbd dub ebd ebd ssd dds grandfather sas

1200

SBU Tgr 385 Ръе Zeg C1u C1p 390 Aba Wa1 Ya1 Azr Lye 395 Bie Rgo SBU Aba Nbz 400 abs asd grandfather grandfather garden dbe dad garden dad ebd dds dad sbs ace ddb saa 1248 Not Bj Aba Aba Sbp OUB Sbi Sbp Sbi Bie SBU Sbi Bie Tht SBU Sbp 405 410 415 sbs dds sbs yeah whiter yeah asd garden das dds dub bda sds bbe eating bbe

1296

Ley SBU Bie SBI ZEG SBI TRG SBP Azr SBU Azr Suz Agd Rye Agd Rye 420 425 430 ace das dub asd ebd ssd dbe women ebd bdd ebd abd abs dds sds ebd 1344 Thr Azr Azr Bj Bie Rgo OUB Tug Bie Tgr Bie Meb Not SBU Agd Bie 435 440 445 sds sbs das hell dds asd ebd yeah das grandfather ebd dss ace dbe bda

1389

Aghd Ley Azr Zeg Sbu Thb Ley Sbi Azr Aba Ley Aba Thb Wab * 450 455 460

<210> 17 <211> 462 <212> RCT

- 55 015170 <213> Sp. ATCC53159 <220>

<221> REVERTE <222> (0) ... (0) <223> ipkomp <400> 17

VaB A1a VaB Siu Zeg A1a Bie Agd RNE Yee 10 Tgr Rgo RNe Sue Agd fifteen Agd one 5 SI SI Rgo SI SI SI Sue Tug RNe Rgo Bie TNG Aba TNG Yb Yb twenty 25 thirty Rgo Hb Agd Azr Aba Hb Zeg Sue Agd Sue Agd Rgo Azr RNe Rgo TNG 35 40 45 Pb Agd Aba Zeg Aba Bie Azr Agd Rgo Bie Azr Agd Agd Agd Azr SI fifty 55 60 Agd Agd SI Not YOU Agd Aba Agd RNe Aba Bie Aba TNG RNe RNe TNG 65 70 75 80 Rgo Tht Bn Rgo Yb Aba Azr Agd Zeg Zeg RNe Aba Sue Agd Bie Sue 85 90 95 Yb Bie A1a Agd Bie Not Zeg Zeg 11th SI Zeg Bn Agd Zeg Hb Yb one hundred 105 110 Yb Bie Tug Sue SI Agd Sue 11th Sue Bz TNG Zeg Bie Bie Hb Yb 115 120 125 Bie Tht Azr Yb Aba Agd SI Zeg Zeg Tug Not Yb Zeg Tug Aba TNG 130 135 140 Suz Sue Aba Azp Aba Azp RNe Zeg Azr Yb RNe Agd Aba Yb Bie SI 145 150 155 160 Azr Yb Rgo Bie Bie Pb Hb Agd Sue Yb A1a Rgo Azp Aba Xiu SI 165 170 17 5 Aba SI Zeg Sue Sue Azp Bie Aba Azr Agd Bie Rgo TNG Sue Zeg RNe 180 185 190 Sue Rgo Sue SI Bie Aba Azr Bie Suz Aba Azr Not TNG Sue TNG Agd 195 200 205 Ya1 Bie Not 11th Bie Azr SI Tug Azr Agd Yb Zeg Azr Zeg Aba RNe 210 215 220 Agd Bn Bn Yb Aba SI Bie Not Bz Azp Bie Zeg Azr Agd Zeg Aba 225 230 235 240 Agd Yb Bn Bie Yb 11th Aba Sue Yb Aba Zeg Azp Bie Bn SI Bie 245 250 255 Not Sue Tug Aba Rgo Zeg Not Agd Agd Azp Yb Not Sue Bie Rgo Mek 260 265 270 Rgo Agd Bie SI SI Zeg SI Yb Bn SI MeE 11th Aba Bie Sue SI 275 280 285 Tht Aba Zeg Sue Yb Agd RNe Azr Rgo Azr Bie TNG Hb MeE Pe Hb 290 295 300 Bie Bie Aba Bie Sue Zeg Rgo Tug RNe Aba Agd Bie Bie Suz Hb Hb 305 310 315 320 Zeg Aba Bie SI Aba Bie Azr Bn Sue Agd Bie TNG Yb Azr Aba Sue 325 330 335 Hb Bie Agd Agd Aba Bie Azr Bn Aba Not Bie SI Not SI Sue Agd 340 345 350 MeE Rgo Rgo Agd Aba Yb Not SI Mek Agd Bz RNe Yb Sue Sue Agd 355 360 365 Tug Azr Rgo Bie Yb Aba Aba Bie Sue SI Aba Zeg Agd Zeg Aba Azr 370 375 380 Sue Tgr Pb Zeg Sue Bn Aba Yb Yb Azr Bie Bie Rgo Sue Aba Hb

- 56 015170

385 390 395 400 S Tht A1a Aba Bn Yb SI Bn SI Bie Sue SI Bie Tht Sue Bn 405 410 415 Bie Sue Bie SI Zeg SI Tht Bn Azr Sue Azr Suz Agd Pb Agd Pb 420 425 430 YOU Azr Azr Tht Bie Rgo Yb Tug Bie Tgr Bie Mek Bb Sue Agd Bie 435 440 445 Agd Bie Azr Zeg Sue Tht Bie SI Azr Aba Bie Aba Tht Yb 450 455 460

<210> 18 <2Ы> 1299 <212> ϋΝΑ <213> ATCC53159 <220>

<221> POPs <222> (1). .. (1299) <223> drzG <400> 18

ak aad 48 sed Rgo ada sss ddd dda ace kkk akd saa dka aac kx aac food Agd Mek B Bz Agde Rgo 5 Sue Sue Tht Pb Mek 10 Bn Yb Azp Pb Azp fifteen garden Dec sds aad SCS dd dss dds aac grandfather SCS grandfather edd ddd ddd sss 96 Bn Aba Agd Bz Bie Sue Aba Sue Azp Aba Bie Aba Agd Sue Sue Rgo twenty 25 thirty d ^ d ekk grandfather skd ekk grandfather ace grandfather dsa cdd asa saa ssk grandfather skd grandfather 144 Yb Bie Aba Bie Bie Aba YOU Aba Aba Tgr Tht Bn Rgo Aba Bie Aba 35 40 45 garden food garden dsa kkk dad css sds sss css ddk hell dad food garden dke 192 Bn Agd Bn Aba Pb SI Zeg Agd RGO Zeg Sue Zeg SI Agd Bn Yb fifty 55 60 give aki sds grandfather asd ddd sneaker skd yeah as das das aas at IP I teach skd 240 Azr S Agd Aba Tht Sue Zeg Bie SI Tug Azr Azr Azp Yb Yb Bie 65 70 75 80 aas das garden edd ax asd das dds grandfather caustic dds duck dkd ax dsa sneaker 288 Azp Azr Bn Agd S Tht Azr Sue A1a Agd Sue Azr Yb Bb Aba Zeg 85 90 95 sss ddd skd das dkd ace ska dkk skd sss sds dss ace ddd garden SCS 336 Rgo Sue Bie Azr Yb Tht Bie Yb Bie Rgo Agd Aba Tht Sue Bn Bie one hundred 105 yo

- 57 015170

cas 384 Tug SCS Bie ace dds TNG 61u 115 aak dke dda 61y as Tug 120 eating Agd kkk RNe cas Tug aad food like ace TNG aas Azp Azp Ya1 Bz Agd 125 Tug kkk aas sds dad garden ax sneaker SCS ace dds dds dsa duck garden edd cx 432 RNe Azp Agd O1i 61p Not Zeg Bie Tht C1u 61y A1a Azr C1p Agd RNe 130 135 140 dss css kde dke dkd sas ddd yeah dke dds as garden eating sas SCS ace 480 A1a Zeg Suz Ya1 Ya1 H13 61y C1 and Ya1 61y Tug C1p Agd H13 Bie TNG 145 150 155 160 das skd css hell ax ccd ax garden das ace asd ssk grandfather SCS aas aas 528 Azr Bie Zeg Zeg Not Bie Not 61p Azr TNG TNG Rgo A1a Bie Azp Azp 165 170 175 ace yeah dad dss edd garden cas ace grandfather duck ax dds kde dds grandfather ace 576 TNG 61and C1 and A1a Agd 61p Tug TNG A1a Azr Not C1u Suz C1u A1a TNG 180 185 190 cas dds skd edd ssk dss dkk css cas ace sds aas yeah dd sds aas 624 Tug 61y Bie Agd Rgo A1a Ya1 Zeg Tug TNG Agd Azp 61and Ya1 Agd Azp 195 200 205 hell ekk dss dad sds food cas grandfather das sneaker aac ace aas ace kkk ace 672 Zeg Bie A1a C1 and Agd Agd Tug A1a Azr Zeg Azp TNG Azp TNG RNe TNG 210 215 220 dsa garden ekk dds skd ask sneaker ssk dss skd ddd ace dnd grandfather dka kkk 720 A1a 61p Bie 61y Bie TNG Zeg Rgo A1a Bie 61y TNG Ya1 A1a Ya1 RNe 225 230 235 240

ddd sdk akd kss das adé ad

768

SZ-U Agd MeE Zeg Azr Zeg 5 © g

245 ace dds garden das ddd akd aad 816

TNG 61u 61p Azr 61u Meck

260

EAE dke sack eating dke ekk STS dds acc Boo ' Ya1 H1z Dgggg *** 75 Ya1 Τ-θνι Rgo 61 V Not - l - 250 255 hell cas grandfather dss dds dke garden SCS dad Zeg Tug 265 A1a A1a 61y Ya1 61p 270 Bie 61and

sds sneaker dkd dss aas food SCS sack cx aas dds sneaker dkd aac cas ace 864 Agd Zeg Ya1 A1a Azp Agd Bie ΗΪ3 RNe Azp 61y Zeg Ya1 Azp Tug TNG 275 280 285 dad dkk das ssa aad SCS dsa css ace aah dda cx aad dds dka dda

912

- 58 015170

C1 and Wa1 Aug 290 Rgo Bz Bie A1a Zeg 295 Tht Bz C1u pb 300 Bz C1u Ya1 C1u B aas DEER Ess dds yes Eab DSE ddE yes garden Eae hell CEC saa Food 960 Pb Azp Ya1 Zeg C1u Azr Tug A1a C1u Azr 61p Tug Zeg Bie C1p Bie 305 310 315 320 eat DSE Esa food Bcd sss garden CCE Esa CEE CEE eat EEC grandfather dde Eae

1008

Bie A1a Zeg Agd Zeg 325 Rgo C1p Rgo Zeg Bie 330 Еи еи Pb Ya1 C1u 335 Tug dad aee grandfather asa grandfather DEER Eating grandfather aaE grandfather asd sds sdd eat hell yes 1056 11th Ya1 Tht A1a Ya1 Zeg A1a Azp A1a Tht Agd Agd Bie Zeg Azr 340 345 350 sds aee garden aaa Eating eat saa dds hell food ace edd sds dad cEC grandfather 1104 Agd Not C1p 11th Zeg Bie C1p C1u Zeg Agd Tht Tgr Agd C1 and Bie A1a 355 360 365 ECE Eating sdd eat cEC ace aas grandfather ssd aee Ess dds aas das aas ace 1152 Zeg Zeg Agd Bie Bie Tht Azp Ya1 Rgo Not 5eg 61U Azp Azr Azp Tht 370 375 380 Eating asd Food EEC dss Ess DSE ace EEC sdd ssd aaE sds sdd eat hell 1200 Zeg Tht Bie Pb A1a Zeg A1a Tht Pb Agd Rgo Azp Agd Agd Bie Zeg 385 390 395 400 Eee grandfather eat dde dss dds CEE garden sdd sds ace hell aas asd garden cea 1248 Pb Ya1 Bie C1u A1a C1u Bie C1p Agd Agd Tht Zeg Azp Tht 61 p Bie 405 410 415 Eae hell Eae hell Ess aah sds aec aaE cEC Eating asd Eating CEE Eating cEC 1296 Tug Zeg Tug Zeg Zeg Bz Agd Not Azp Bie Zeg Tht Zeg Bie Zeg Bie 420 425 430

Food 1299 *

<210> 19 <211> 432 <212> RKT <213> ATCC53159 <220>

<221> REVERTE <222> (0) ... (0)

- 59 015170 <223> ipkpoip <400> 19

Mek bz one Rgo Agde Рgo СЬу 5 Sue Tkg Rke Mek 10 Yb Azp Rke Azp fifteen Agd Bn Aba Agd Bz Bie Sue A1a C1u Azp A1a Bie Aba Agd C1u C1u Rgo twenty 25 thirty Yb Bie Aba Bie Bie Aba Tkg Aba Aba Tgr Tkg Bn Rgo Aba Bie Aba 35 40 45 Bn Agd Bn Aba Rke SI Zeg Agd Rgo Zeg C1u Zeg SI Agd Bn Yb fifty 55 60 Azr Not Agd Aba Tkg C1u Zeg Bie SI Tug Azr Azr Azp Yb Yb Bie 65 70 75 80 Azp Azr Bn Agd Not Tkg Azr Sue A1a Yeah Sue Azr Yb Not Aba Zeg 85 9θ 95 Rgo Sue Bie Azr Yb Tkg Bie Yb Bie Rgo Agd Aba Tkg Sue Bn Bie one hundred 105 BUT Tug Bie Tkg C1u Azp Yb Sue Tug Agd Rke Tug Bz Agd Tug Tkg Azp 115 120 125 Rke Azp Agd SI Bn Not Zeg Bie Tkg Sue Sue A1a Azr Bn Agd Rke 130 135 140 Aba Zeg Suz Yb Yb Hb Sue SI Yb Sue Tug Bn Agd Hb Bie Tkg 145 150 155 160 Azr Bie Zeg Zeg 1b Bie Not C1p Azr tkg Tkg Rgo Aba Bie Azp Azp 165 170 175 Tkg SI SI Aba Agd C1p Tug Tkg A1a Azr Not C1u Suz Sue Aba Tkg 180 185 190 Tug Sue Bie Agd Rgo Aba Yb Zeg Tug Tkg Agd Azp SI Yb Agd Azp 195 200 205 Zeg Bie A1a SI Agd Agd Tug A1a Azr Zeg Azp Tht Azp Tht Rke Tkg 210 215 220 Aba Bn Bie Sue Bie Tkg Zeg Rgo Aba Bie C1u Tkg Ya1 Aba Yb Rke 225 230 235 240 C1u Agd Mek Zeg Azr Zeg Zeg Tug Yb ΗΪ3 Agd Yb Bie Rgo Sue Bb 245 250 255 Tkg Sue Bn Azr Sue Mek Bz Zeg Tug Aba Aba C1u Yb C1p Bie SI 260 265 270 Agd Zeg Yb A1a Azp Agd Bie H1z Rke Azp C1u Zeg Yb Azp Tug Tkg 275 280 285 SI Yb Azr Rgo Bz Bie Aba Zeg Tkg Bz Sue Rke Bz Sue Yb Sue 290 295 300 Rke Azp Yb Zeg C1u Azr Tug Aba Sue Azr Bn Tug Zeg Bie Bn Bie 305 310 315 320 Bie Aba Zeg Agd Zeg Rgo Bn Rgo Zeg Bie Bie Bie Rke Yb Sue Tug 325 330 335 SI Bb Yb Tkg Aba Yb Zeg A1a Azp Aba Tkg Agd Agd Bie Zeg Azr 340 345 350 Agd Bb Bn B1e Zeg Bie Bn Sue Zeg Agd Tkg Tgr Agd SI Bie Aba 355 360 365 Zeg Zeg Agd Bie Bie Tkg Azp Yb Rgo Not Zeg Sue Azp Azr Azp Tkg 370 375 380 Zeg Tkg Bie Rke Aba Zeg A1a Tkg Rke Agd Rgo Azp Agd Agd Bie Zeg 385 390 395 400 Rke Yb Bie Sue Aba C1u Bie Bn Agd Agd Tht Zeg Azp Tkg Bn Bie 405 410 415 Tug Zeg Tug Zeg Zeg Bz Agd Not Azp Bie Zeg Tkg Zeg Bie Zeg Bie 420 425 430

- 60 015170 <210> 20 <211> 918 <212> LNA <213> ATCC53159 <220>

<221> pops

<222> (1) .. <223> others . (918) <400> 20 aU sae aec aad aaE sds EEC d £ d aaE aec Esd asd Food dss aec dss 48 MeE Nbz bbe Bz Azp Agd Pb OUB Azp bbe Zeg Tht Bie Aba bbe Aba one 5 10 fifteen dss grandfather eat dss asd ssd grandfather grandfather grandfather garden aec sss asd sdd Ess grandfather 96 Aba Aba Bie Aba Tht Rgo Aba Aba Aba C1p Not Rgo Tht Agd Zeg OUB twenty 25 thirty sss grandfather ssd dss sds ssd edd sce dsa asd ssd ssd grandfather saa garden garden 144 Rgo Aba Rgo Aba Agd Rgo Agd Rgo Aba Tht Rgo Rgo Aba Sbp Sbp Sbp 35 40 45 aas garden grandfather ssd Esd asd sss dsa grandfather dsa ace ssd grandfather garden ace dss 192 Azp Sbp Aba Rgo Zeg Tht Rgo Aba Aba Aba Tht Rgo Aba Sbp Tht Aba fifty 55 60 dsa ace DEER dss sce dsa dsa ace dsa sss dsa ddE Eas aah aec dds 240 Aba Tht OUB Aba Rgo Aba Aba Tht Aba Rgo Aba SBU Tug Bz bbe SBU 65 70 75 80 grandfather das das grandfather aec dad dss das grandfather cEC dds garden ace das EEC aad 288 OUB Azr Azr OUB bbe Sbi Aba Azr OUB Bie SBU Sbp Tht Azr Pb Bz 85 90 95 asd sds dss cde grandfather garden grandfather das dds asd grandfather ace eat sss EAE eat 336 Tht Agd Aba Agd OUB Sbp Aba Azr SBU Tht OUB Tht Bie Rgo Tug Bie one hundred 105 110 dds dss d ^ d garden des aad dds dad ace grandfather ace Esd cEC dss yeah aad 384 SBU Aba OUB Sbp OUB Bz SBU Sbi YOU Aba Tht Zeg Bie Aba Sbi Bz 115 120 125 with food dss ddd eat eat sds dss dds dds EAE EAE dss aad ssd aec des 432 Bie Aba SBU Bie Bie Agd Aba SBU SBU Tug Tug Aba Bz Rgo bbe OUB

130 135 140

- 61 015170

hell des yeah aes des dd ees des ada aas EAE FOOD ASD DEED eat Bie dde C1u 160 480 5eg Wa1 145 C1 and 11th Ya1 Zeg Azp Tht Ya1 C1u 150 Pb Ya1 Tug 155 Ya1 garden grandfather aas hell Ess dde eat garden ssd des das sds dde as sas DEER 528 61p Ya1 Azp Zeg Zeg 61y Bie C1p Rgo Ya1 Azr Agd C1u Tug Nhz Ya1 165 170 175 Ess dad aec aec dss cde dss dde dde eat eating sss yeah grandfather dss duck 576 Zeg C1 and 11th Not A1a Agd A1a C1u 31u Bie Agd Rgo 61and A1a A1a Azr 180 185 190 EEC des DEER CEC ace sds dss yes dde Ess hell dss aad eat das Eae 624 Pb Ya1 Ya1 Bie Tht Agd A1a Avr 61y Zeg Zeg A1a Bz Bie Azr Tug 195 200 205 aad aad CEC dss saa ddE dde sss aac das yes ssd akd d ^ d asd sss 672 Bz Bz Bie A1a C1p C1u C1u Rgo Azp Azr Azr Rgo Mek Ya1 Tht Rgo 210 215 220 ddd das aad des Eee des ssd yeah des dad sae EEC Eae acc EAE ddE 720 C1u Azr Bz Ya1 Pb Ya1 Rgo 61and Ya1 C1 and Nhz RNe Tug Not Tug C1u 225 230 235 240 saa aee aas grandfather ssk dde yeah Eae grandfather aee food sneaker das akd asd cEC 768 C1p Not Azp A1a Rgo 31u Ya1 Tug A1a Not Agd Zeg Azr Mek Tht Bie 245 250 255 cde sds grandfather eat dss garden dde ddE ddd CEE dss sss dsa dde Ess dke 816 Agd Agd A1a Bie A1a 61p 61y 31u 61y Bie A1a Rgo A1a 61y Zeg Ya1 260 265 270 aad cde grandfather aad des asd edd yes dde aaE yeah cEC aad Food aad skd 864 Bz Agd Ya1 Bz Ya1 Tht Agd Azr C1u Azp C1 and Bie Bz Bie Bz Bie 275 280 285 das duck ssd aee cEC ssa dde das asd aec des aec dde yeah food Food 912 Azr Azr Rgo Not Bie Rgo C1u Azr Tht Not Ya1 Not 61y 31and Agd Bie 290 295 300

EU Food

918

Pb *

305 <210> 21

- 62 015170 <211> 305 <212> RKT <213> Sp. ATCC53159 <220>

<221> RERTYUE <222> (0) .., (0) <223> RISASBUE ROBUZASSASAGBYE EXHROG RGOBEBP <400> 21

Meb one Nbz 11th baz azp 5 Agd Rye Uab Azp bbe 10 Zeg Tg Lei Aba Not aba fifteen Aba Aba Bie Aba TNG Rgo Aba Aba Aba Sbp Not Rgo Bj Agd Zeg OUB twenty 25 thirty Rgo Aba Rgo Aba Agd Rgo Agd Rgo Aba Bj Rgo Rgo Aba Sbp Sbp Sbp 35 40 45 Azp Sbp Aba Rgo Zeg Bj Rgo Aba Aba Aba TNG Rgo Aba Sbp Bj Aba fifty 55 60 Aba Bj OUB Aba Rgo Aba Aba Bj Aba Rgo Aba SBU Tug Bz Not SBU 65 70 75 80 OUB Azr Azr OUB bbe Sbi Aba Azr OUB Bie SBU Sbp Bj Azr RNe Bz 85 90 95 Bj Agd Aba Agd OUB Sbp Aba Azr SBU Tht OUB TNG Bie Rgo Tug Bie 600 105 110 SBU A1a OUB Sbp OUB Bz SBU Sbi Bj Aba Bj Zeg Bie Aba Sbi Bz 115 620 125 Bie Aba SBU Bie Bie Agd Aba SBU SBU Tug Tug Aba Bz Rgo Not OUB 130 135 140 g. τ τ 1 Ί GT 1 ην - g r- ι GP tu-, 1 gpi T l. AND? t. ate νοί ate 116 ν al ate GIS ν al □ C1 AR 11 blasphemy ναι l ph and 145 650 155 160 Sbp OUB Azp Zeg Zeg SBU Bie Sbp Rgo OUB Azr Agd SBU Tug Nbz OUB 665 170 175 Zeg Sbi Not bbe Aba Agd Aba SBU SBU Bie Agd Rgo Sbi A1a Aba Azr 680 185 190 Ръе OUB OUB Bie Bj Agd Aba Azr SBU Zeg Zeg Aba Bz Bie Azr Tug 195 200 205 Bz Bz Bie Aba Sbp SBU SBU Rgo AZP Azr Azr Rgo Month OUB Bj Rgo 210 215 220 SBU Azr Bz OUB Ръе OUB Rgo Sbi OUB Sbi Nbz RNe Tug Not Tug SBU 225 230 235 240 Sbp 11th Azp Aba Rgo SBU OUB Tug Aba 11th Agd Zeg Azr MeC TNG Bie 245 250 255 Agd Agd Aba Bie Aba Sbp SBU SBU SBU Bie Aba Rgo Aba SBU Zeg OUB 260 265 270 Bz Agd OUB Bz OUB Bj Agd Azr SBU Azp Sbi Bie Bz Bie Bz Bie 275 280 285 Azr Azr Rgo bbe Bie Rgo SBU Azr Bj Not OUB Not SBU Sbi Agd Bie 290 295 300 Ръе 305 <210> 22 <211> 1347 <212> ϋΝΑ <213> ZrIbpdotopaz sp. ATCC53159 <220>

- 63 015170 <221> POPs <222> (1). . . (1347) <223> brzs <400> 22

grandfather 48 Ya1 one aaE Azp aec Not aee Not garden 61p 5 EEC Pb EEC Ръе sds aee eat Bie 10 dd Tgr DEED SDS food Agd Unit Tgr fifteen aec Not Ya1 Agd Agd 11th aec cEC ssd grandfather Eee cEC DEER Food DEER ace acE dss ace aee grandfather des 96 Not Bie Rgo A1a Pb Bie Ya1 Suz Ya1 Tht Tht A1a Tht Not Ya1 Ya1 twenty 25 thirty garden Eee eat sss yeah sds Eas aad dss acE asd sdd grandfather grandfather cEC das 144 61p Pb Bie Rgo C1 and Agd Tug Bz A1a Tht Tht Agd Ya1 Ya1 Bie Azr 35 40 45 asd Eee aad sss yes sss des ace dda garden grandfather aU hell Esd garden EEC 192 Tht Pb Bz Rgo Azr Rgo Ya1 Tht <31u C1p Ya1 MeE Zeg Zeg 01p ₽ fifty 55 60 aU sds dss EAE des dad acE garden ace garden eat aec dad das EAE grandfather 240 MeE Agd A1a Tug Ya1 C1 and Tht C1p Tht C1p Bie 11th 61and Azr Tug A1a 65 70 75 80 ace dss ddE sds d ^ d des das yeah eat dds Unit grandfather aaE yes ssd grandfather 288 Tht A1a C1u Agd Ya1 Ya1 Azr 01and Bie C1u Tgr Ya1 Azp Azr Rgo A1a 85 90 95 aas aec Ess grandfather EEC aas aas Esd Ess grandfather DSE dss ace dds das aec 336 Azp Not Zeg A1a Pb Azp Azp Zeg Zeg A1a A1a A1a Tht C1u Azr Not one hundred 105 but sds sds Unit CEC dss aad garden aec aec das aaE ace aad dss yes grandfather 384 Agd Agd Tgr Bie A1a Bz C1p Not Not Azr Azp Tht Bz A1a Azr Ya1 110 ιζυ 1ΖΟ aU dad ddd hell aas aec cEC yeah aec ace EAE Esd das hell Esd sss 432 MeE C1 and C1u Zeg Azp Not Bie 61and Not Tht Tug Zeg Azr Zeg Zeg Rgo 130 135 140 dad sds dss yeah sds aec dss aas eat aec sds ace Esd EEC cEC dss 480 C1 and Agd A1a <31and Agd 11th A1a Azp Bie 11th Agd Tht Zeg Pb Bie A1a 145 150 155 160 garden Esd cEC dss dss aad sds garden dss grandfather ace aad Esd dss das Unit 528

- 64 015170

61η Zeg bie A1a A1a 165 Bz Agd 61p A1a A1a Tkg 170 Bz Zeg A1a Azr 175 Tgr cas dss garden garden dss yeah Dec dss sds duck sneaker SCS Dec grandfather grandfather dks 576 Tug A1a 61p 61p A1a 61and A1a A1a Agd Azr Zeg Bie A1a A1a A1a Ya1 180 185 190 garden dss sds ace duck cx dkd aad aad ace dds ax dkd skd ace yeah 624 61p A1a Agd Tkg Azr Rke Ya1 Bz Bz Tkg 61y Not Ya1 Bie Tkg 61and 195 200 205 ace dds dss das skd yeah ace garden aad SCS garden garden ax dad ddd garden 672 Tht C1u A1a Azr Bie 61and Tkg 61p Bz Bie 61p 61p Not O1i C1u 61p 210 215 220 asd asd ace dss ace dss ssd dkk dss akd dss sss hell dds akd dds 720 Tkg Tkg Tkg A1a Tkg A1a RGO Ya1 A1a Mek A1a Rgo Zeg 61y Mek C1u 225 230 235 240 sed grandfather garden akd garden SCS dss garden ax das garden garden ax garden garden dsa 768 Rgo A1a 61p Mek 61p Bie A1a C1p 11th Azr 61p 61p 11th 61p 61p A1a 245 250 255 grandfather ace hell ska ddk ssd aas sas ssa ask cx garden dss ccd ed edd 816 A1a Tkg Zeg Bie 61y Rgo Azp ΗΪ3 Rgo Tkg Rke 61p A1a Bie 61p Agd 260 265 270 garden sds yeah dkd cx dss aad dsa grandfather grandfather grandfather yeah sds grandfather garden grandfather 864 61p Agd 61and Ya1 Rke A1a Bz A1a A1a A.1a A1a 61and Agd A1a 61p A1a 275 280 285 aas dds dka css dd ssd dsa sds ddd dss ax yeah hell dsa dss aas 912 Azp C1U Ya1 Zeg 61y Rgo A1a Agd 61y A1a Not 61and Zeg A1a A1a Azp 290 295 300 dss garden sds grandfather edd dkk SCS dds aac caustic garden duck dks das aad ekk 960 A1a 61p Agd A1a Agd Ya1 Bie C1u Azp Agd 61p Azr Ya1 Azr Bz Bie 305 310 315 320 asd garden sed garden caustic das dks sneaker skd aad garden duck garden cas akd aad

1008

Tkg 61p Bie 61p Agd Azr Ya1 Zeg Bie Bz 61p Azr C1p Tug Mek Bz 325 330 335 grandfather dsa 1056 garden sds dks dss duck skd edd skd yeah dsa hell hell aas duck A1a A1a 61p Agd Ya1 A1a Azr Bie Agd Bie C1 and A1a 5eg Zeg Azp Azr

340 345 350

- 65 015170

dke dds akd sneaker asd SCS hell yeah dsa sneaker grandfather ssd yeah asd sss as 1104 Wa1 C1u Mek Zeg Tht Bie Zeg SI Aba Zeg Aba Rgo SI Tht Rgo Tug 355 360 365 cas sss aad dkd ssd SCS ax ax ddk dde dsa dss dds cx dds SCS 1152 Tug Rgo Bz Yb Rgo Bie S S Sue Sue Aba Aba Sue Pb Sue Bie 370 375 380 ddd sks ddk skd skd dke grandfather skd SCS dke dad skd SCS dds sds sds 1200 C1 y Sue Bie Bie Yb A1a Bie Bie Yb SI Bie Bie Sue Agd Agd 385 390 395 400 dke sds hell sss dad duck skd yeah dkk grandfather ax duck dsa ssd dkd skd 1248 Vaib Agd Zeg Rgo SI Azr Bie SI Yb Aba S Azr A1a Rgo Yb Bie 405 410 415 dds dkd ax garden hell sds dss sneaker ekk dss dss sds ekk sds sds dss 1296 C1y Not Bn Zeg Agd Aba Zeg Bie Aba Aba Agd Bie Agd Agd Aba 420 425 430 saa daa ace SCS dds yeah dd dss das asd sas dda Dec ksa dka aas 1344 Bn b Tht Bie Sue SI Sue Aba Azr Tht Hb Sue Aba Zeg Yb Azp 435 440 445

to yes

1347 *

<210> 23 <211> 448 <212> RKT <213> ATCC53159 <220>

ηυτγπτηΐΐ χώώΐ- 'of the glossy <222> (0) ... (0) <223>

Ya azp one S S Bn 5 Rye Rye Agd Bb Bie 10 Tgr Tgr fifteen S S Bie Rgo Aba Pb Bie Yb Suz Yb Tht YOU Aba Tht S Yb Yb twenty 25 thirty Bn Pb Bie Rgo SI Agd Tug Bz Aba Tht Tht Agd Yb Yb Bie Azr 35 40 45 Tht Pb Bz Rgo Azr Rgo Yb Tht Sue Bn Yb Mek Zeg Zeg Bn Pb fifty 55 60 Mek Agd Aba Tug Yb SI Tht Bn Tht Bn Bie B 1e SI Azr Tug Aba

- 66 015170

65 70 75 80 Tkg A1a C1u Agd Ya1 Ya1 Azr 61and Bie 61y Tgr Ya1 Azp Azr Rgo A1a 85 90 95 Azp Not Zeg A1a Rke Azp Azp Zeg Zeg A1a. A1a A1a Tkg 61y Azr Not one hundred 105 but Agd Agd Tgr Bie A1a Bz 61p Not Not Azr Azp Tkg love A1a Azr Ya1 115 120 125 Me 61and 61y Zeg Azp Not Bie 61and Not Tkg Tug Zeg Azr Zeg Zeg Rgo 130 135 140 61and Agd A1a 61and Agd Not A1a Azp Bie Not Agd Tkg Zeg Rke Bie A1a 145 150 155 160 61p Zeg Bie A1a A1a Bz Agd 61p A1a A1a Tkg Bz Zeg A1a Azr Tgr 165 170 175 Tug A1a 61 p 61p A1a 61and A1a A1a Agd Azr Zeg Bie A1a A1a A1a Ya1 180 185 190 61p A1a Agd Tkg Azr Rke Ya1 Bz Bz Tkg 61y Not Ya1 Bie Tkg 61and 195 200 205 Tkg 61y A1a Azr Bie 61and Tkg 61p Bz Bie 61p 61p 11th 61and 61y 61p 210 215 220 Tkg Tkg Tkg A1a Tkg A1a Rgo Ya1 A1a Mek A1a Rgo Zeg 61y Mek 61y 225 230 235 240 Rgo A1a 61p Mek 61p Bie A1a 61p Not Azr 61p 61p Not 61p 61p A1a 245 250 255 A1a Tkg Zeg Bie 61y Rgo Azp Nhz Rgo Tkg Rke 61p A1a Bie 61p Agd 260 265 270 61p Agd 61and Ya1 Rke A1a Bz A1a A1a A1a A1a 61and Agd A1a 61l A1a 275 280 285 Azp C1u Ya1 Zeg 61y Rgo A1a Agd C1u A1a Not 61and Zeg A1a A1a Azp 290 295 300 A1a 61p Agd A1a Agd Ya1 Bie 61y Azp Agd 61p Azr Ya1 Azr Bz Bie 305 310 315 320 Tkg 61p Bie 61p Agd Azr Ya1 Zeg Bie Bz 61p Azr 61p Tug MeB Bz 325 330 335 A1 a A1a 61 p Agd Ya1 A1a Azr Bie Agd Bie 61and A1a Zeg Zeg Azp Azr 340 345 350 Ya1 61y Mek Zeg Tkg Bie Zeg 61and A1a Zeg A1a Rgo 61and Tkg Rgo Tug 355 360 365 Tug Rgo Bz Ya1 Rgo Bie Not Not 61y 61y A1a A1a 61y Rke 61y Bie 370 375 380 61y Bie 61y Bie Bie Ya1 A1a Bie Bie Ya1 61and Bie Bie 61y Agd Agd 385 390 395 400 Ya1 Agd Zeg Rgo 61 and Azr Bie 61and Ya1 A1a 11th Azr A1a Rgo Ya1 Bie 405 410 415 C1u Ya1 Not 61p Zeg Agd A1a Zeg Bie A1a A1a Agd Bie Agd Agd A1a 420 425 430 C1p 61and Tkg Bie 61y 61and C1u A1a Azr Tkg Nhz 61y A1a Zeg Ya1 Azp 435 440 445

<210> 24 <211> 708 <212> ΟΝΑ <213> Zrkhpdotopaz sp, ATCC53159.

<220>

<221> POPs <222> (1) .., (708) <223> ErzE

- 67 015170

<400> 24 ASD 48 MeC one das grandfather ace ada yeah ssd Rgo ssd sss yeah dds yes od C Agd ssd Rgo fifteen hell Zeg 61and C1u Azr Azr A1a MeC YOU 5 Zeg C1 and Bie Rgo 10 dss dsd ssd ace asd ssd yes s asd ACC dds asd ssc yeah Sas garden ssc 96 A1a Ya1 Rgo Tht YOU Rgo Azr Tht Not 51u MeC Bie 61and Tug C1p Bie twenty 25 thirty dss ssc Sss yes ssd ace ddd ACC dad grandfather yeah grandfather ACC sds grandfather cSa 144 Ya1 Bie Zeg Azr Rgo Tht 61y Not 61and A1a C1 and A1a Not Agd A1a Bie 35 40 45 sds asd sds ACC ASD ace garden sas ssc sds dad dds sdd sds grandfather ssc 192 Agd TNG Agd Not MeC TNG C1p H1z Bie Agd C1 and C1u Agd Agd A1a Bie fifty 55 60 grandfather ACC Vds dss dss Ssd grandfather dda Sss dds Vds hell SSc ace dss dss 240 A1a Not Suz A1a A1a Zeg A1a C1u Zeg C1u Suz Zeg Pb TNG A1a Ya1 65 70 75 80 aaS cd grandfather asd grandfather csd grandfather garden ACC dds DSS aad ac grandfather csd dss 288 Azp Bie A1a Tht A1a Bie A1a C1p Not 61y Ya1 Bz Tht A1a Bie Ya1 85 90 95 yes dss aaS csd sds Dan sss hell ACC dds dsa dss SSc dds ssc dss 336 Azr A1a Azp Bie Agd Azr Rgo Zeg Not C1u A1a A1a Pb s1u Bie A1a one hundred 105 but dss das aad sss dds csd dss das CaS ssc dss Ssd dds yes dss das 384 A1a Azr Bz Rgo 61y Bie A1a Azr Tug Bie A1a Zeg C1u Azr Ya1 Azr 115 120 125 ssc dss Ssd ACC ACC caS grandfather ace sds ssc das garden ssc Ssd ACC ACC 432 Bie A1a Zeg Not Not ΗΪ3 A1a Tht Agd Bie Azr C1p Bie Zeg 11th 11th 130 135 140 ssd dss ddd caS dse dad sas hell ssd garden yeah csd ssc grandfather Sss yeah 480 Rgo A.1a C1u H1z Ya1 61and H13 Zeg Rgo C1p C1 and Bie Bie A1a Zeg C1 and 145 150 155 160 garden SSc caS yes csd grandfather asd garden csd csd sds dad SSc das ACC asd 528 61p Pb H1z Azr Bie A1a Tht C1P Bie Bie Agd C1 and Pb Azr Not Tht 165 170 175

- 68 015170

abc carried das Azr ace Tht 180 asd Tht grandfather Aba ess aae ace Tht 185 food dss das grandfather Aba garden meal Bn agd 190 des Yb 576 Bb Pb Zeg Azp Suz Aba Azr grandfather ooh! abs dss dds eae grandfather abc aes grandfather grandfather sds aad give grandfather hell 624 Aba Hb Not Aba Sue Tug Aba Not Not Yb Aba Agd Bz Azr Aba Zeg 195 200 205 bac abc sds das grandfather aah asd ses hell sds asd sed eating dsa das sds 672 Tug Not Agd Azr Yb Azp Tht Bie Zeg Agd Tht Bie Agd Aba Azr Agd 210 215 220 ace aah des aes dds eating dha sed aah dds eae food 708 Tht Azp Yb 11th 61y Suz Yb Bie Azp Sue Tug * 225 230 235

<210> 25 <211> 235 <212> RCT <213> ATCC53159 <220>

<221> WRITTEN <222> (0) ... (0) <223> е а Ъ у е ро уз асс асс ех рог рог <<<<<<<400> 25

Mee Azr Aba Mee Tht Zeg SI Rgo Bie Rgo SI Sue Azr Agd Rgo Zeg one 5 10 fifteen Aba Yb Rgo Tht Tht Rgo Azr Tht Not Sue Mee Bie SI Tug C1p Bie twenty 25 thirty Yb Bie Zeg Azr Rgo Tht Sue Not SI Aba SI Aba Bb Agd Aba Bie 35 40 45 Agd Tht Agd Not Me Tht Bn Hb Bie Agd SI Sue Agd Agd Aba Bie fifty 55 60 Aba Not Suz Aba Aba Zeg Aba Sue Zeg Sue Suz Zeg Pb Tht Aba Yb 65 70 75 80 Azp Bie Aba Tht Aba Bie Aba Bn Not Sue Yb Bz Tht Aba Bie Yb 85 90 95 Azr Aba Azp Bie Agd Azr Rgo Zeg Bb Sue Aba Aba Pb Sue Bie Aba one hundred 105 110 Aba Azr Bz Rgo C1u Bie Aba Azr Tug Bie Aba Zeg Sue Azr Yb Azr 115 120 125 Bie Aba Zeg Not Not Hb Aba Tht Agd Bie Azr Bn Bie Zeg Bb Bb 130 135 140 Rgo Aba C1u Hb Yb C1 and Hb Zeg Rgo Bn SI Bie Bie Aba Zeg SI 145 150 155 160 Bn Pb Hb Azr Bie Aba Tht Bn Bie Bie Agd SI Pb Azr Bb Tht 165 170 175 Not Pb Azr YOU YOU Aba Zeg Azp Tht Suz Aba Azr Aba C1p Agd Yb 180 185 190 Aba Hb Not Aba C1u Tug Aba Not Bb Yb Aba Agd Bz Azr A1a Zeg

- 69 015170

195 200 205 Tug Not Agd Azr Ya azp YOU Bie Zeg Agd TNG Bie Agd Al Agr Agd 210 215 220 TNG Azp Yb Not 61u Suz Yb Bie Azp Sue Tug 225 230 235

<210> 26 <211> 882 <212> ΟΝΑ <213> ATCC53159 <220>

<221> SRZ <222> (1) ... (882) <223> drzM <400> 26

asd dsa grandfather ace Ala thn grandfather asd sdd TNg Agd garden garden dad add aad dds ddd dds Sue 48 MeC one Aba Bn Bn 10 SI Agd Aba 5 MeC Bz Sue Sue fifteen sas edd eat dss dss dss dde CCC dss grandfather cea ace aec ssd ace EEC 96 Tug Tgr Bie Aba Yb Aba Sue Bie Aba Aba Bie TNG Bb Rgo TNG RNe twenty 25 thirty ACC ace csd dde sds dad dss edd hell grandfather yeah dds dde grandfather garden dde 144 Not TNG Bie Sue Agd SI Yb Tgr Zeg Aba SI Sue Sue Yb Bn Sue 35 40 45 ssd ACC grandfather ssc dss asd dds dss sdd ASD eat dss sds garden Vds Ssd 192 Rgo Not Yb Bie Aba TNG Sue Aba Tgr MeC Bie Aba Agd Bn Suz Zeg fifty 55 60 asd ACC dad grandfather cSa sds sds sss dds hell grandfather eat ssc dds grandfather eat 240 TNG Not SI Aba Bie Agd Agd Rgo Sue Zeg Yb Bie Bie Sue Aba Bie 65 70 75 80 Is csd ssd grandfather asd CCE dss ess Sas ace dss dda sdd grandfather EEC das 288 RNe Bie Bie Aba TNG Bie Aba RNe Tug TNG Yb Sue Agd Yb RNe Azr 85 90 95 Is ACC hell dss yeah ace SSc dda eat des grandfather ace CaE sed des des 336 RNe Not Zeg Yb SI TNG RNe Sue Bie Yb Aba TNG Tug Bie Yb Yb one hundred 105 110 dss CaS ssc EaS EUs dde dss add grandfather ssc sdS dss dss sdd EEC ssd 384 Aba Tug Bie Tug RNe Sue Aba Agd Yb Bie Agd A1a Aba Tgr RNe Rgo 115 120 125

- 70 015170

grandfather 432 Ya1 sed Bie 130 edd Tgr cbd Bie eee Pb eee Pb sed djd ssd ssd sss dds edd dss des Ya1 das Azr Bie 135 Ya1 Rgo Rgo Rgo 61y 140 Tgr A1a sds aes ass dsa ssd ses aad dad eee des ess eae grandfather dsa asd dds 480 Agd Not Tht Aha Rgo Bj Bz 61and Pb Ya1 Zeg Tug A1a A1a YOU 61y 145 150 155 160 sed see ess edd grandfather give eae ssd aes sed sds garden dds dd asa sed 528 Bj Bj Zeg Tgr Ya1 Azr Tug Rgo Not Bie Agd 61p 61 y Ya1 Tht Bie 165 170 175 eee des dds sss eae garden sed ses dke yeah give dss eating eat dde sed 576 Pb Ya1 C1u Rgo Tug 61p Bj Bie Ya1 61and Azr A1a suz Zeg C1u Bie 180 185 190 sds eat sed ess hell sed des des grandfather asd sed ses eas aes eas aes 624 Agd Zeg Bie Zeg Zeg Bie Ya1 Ya1 Ya1 Tht Bj Bie Tug Not Tug Not 195 200 205 aad aas aad ssd ess edd sds eas grandfather grandfather eee aes dsa grandfather sed d ^ d 672 Bz Azp Bz Rgo Zeg Tgr Agd Tug A1a A1a Pb Not A1a A1a Bj Ya1 210 215 220 a £ s ssd grandfather dsa dd grandfather ass aas des sed sdd aes aes abc sed de a 720 11th Rgo Ya1 A1a Ya1 Ya1 Tht Azp Ya1 Bj Agd Not Not Not Bj Ya1 225 230 235 240 sed aes ass eae sae sed dds das dad grandfather grandfather garden hell eee cbc sas 768 Bj 11 © Tht Tug H1z Bj 61U Azr 61and A1a A1a 61p Zeg Pb Bie Ηϊδ 245 250 255 des ess ass dds ad grandfather aed eee grandfather des dss sed see eating abc eee 816 Ya1 Zeg Tht 61y Mee Ya1 Mee Pb Ya1 Ya1 A1a Bie Bj Suz Not Pb 260 265 270 grandfather aes das edd grandfather des dad saa see see ses sed eating sdd add sae 864 A1a Not Azr Tgr Ya1 Ya1 61and 61p Bie Bie Bie Bj Agd Agd Agd Nhz 275 280 285 sae more saa ssd grandfather food 882 H1z Ya1 61l Rgo A1a *

290 <210> 27

- 71 015170 <2Ы> 293 <212> RKT <213> Sp. ATCC53159 <220>

<221> RETURN <222> (0). . . (0) <223> REAL ESSENTIAL NOT EXCEPT PROBE <400> 27

Me A1a Aba Tht Aba me Tht Agd Bn Bn 61and Agd Bz Sue Sue C1u one 5 10 fifteen Tug Tgr Bie A1a Yb A1a Sue Bie Aba Aba Bie Tht Not Rgo Tht Pb twenty 25 thirty S Tht Bie C1u Agd SI Wah Tgr Zeg Aba SI Sue Sue Yb C1p Sue 35 40 45 Rgo Ye Yb Bie Aba Tht C1u A1a Tgr Me Bie Aba Agd C1p Suz Zeg fifty 55 60 Tht Not SI A1a Bie Agd Agd Rgo C1u Zeg Yb Bie Bie Sue A1a Bie 65 70 75 80 Pb Bie Bie A1 a Tht Bie A1a Pb Tug Tht Yb Sue Agd Yb Pb Azr 85 90 95 Pb 11th Zeg Yb SI Tht Pb Sue Bie Ya1 Aba Tht Tug Bie Yb Yb one hundred 105 110 A1a Tug Bie Tug Pb 61y Aba Agd Yb Bie Agd Aba Aba Tgr Pb Rgo 115 120 125 Ya1 Bie Tgr Bie Pb Pb Bie Yb Rgo Rgo Rgo Sue Tgr Aba Yb Azr 130 135 140 Agd Ye Tht Aba Rgo Bie Bz SI Pb Yb Zeg Tug A1a Aba Tht Sue 145 150 155 160 Bie Bie Zeg Tgr Yb Azr Tug Rgo Not Bie Agd Bn Sue Yb Tht Bie 165 170 175 Pb Yb C1u Rgo Tug Bn Bie Bie Yb SI Azr Aba Suz Zeg Sue Bie 180 185 190 Agd Zeg Bie Zeg Zeg Bie Ya1 Yb Yb Tht Bie Bie Tug Not Tug Not 195 200 205 Bz Azp Bz Rgo Zeg Tgr Agd Tug A1a A1a Pb Not A1a Aba Bie Yb 210 215 220 11th Rgo Ya1 Aba Ya1 Yb Tht Azp Yb Bie Agd Not Not Ye Bie Yb 225 230 235 240 Bie Not Tht Tug Hb Bie Sue Azr SI Aba Aba Bn Zeg RNe Bie Hb 245 250 255 Yb Zeg Tht Sue Me Yb Me Pb Yb Yb Aba Bie Bie Suz Not Pb 260 265 270 A1a Not Azr Tgr Ya1 Yb SI Bn Bie Bie Bie Bie Agd Agd Agd Hb 275 280 285

B3 ya1 s1p rgo aa

290 <210> 28 <211> 699 <212> ϋΝΑ <213> ATCC53159 <220>

<221> POPs <222> (1) ... (699)

- 72 015170 <223> άρβΝ <400> 28

akd 48 Mek one Kks aas edd sdk Agd 5 das Azr skd skd Еи еи ax dds dsa Not 61u A1a 10 dds 61y kde Suz cx RNe dss A1a fifteen dss A1a Rne Azp Agd Dec dds dss sneaker SCS dds skd aad sed sas edd edd akd das skd skd 96 A1a 61 y A1a Zeg Bie 61y Bie Bz Rgo H1z Agd Agd Mek Azr Bie Bie twenty 25 thirty dds dds ace aad SCS das asd skd akd sss aad dsa cx dds dsa edd 144 61y C1u TNG Bz Bie Azr TNG Bie Mek Rgo Bz A1a RNe 61y A1a Tgr 35 40 45 aad dsa dad duck ace dde sneaker skd ax grandfather sed grandfather sds yeah dds hell 192 Bz A1a 61and Azr TNG 61y Zeg Bie Not A1a Rgo A1a Agd 61and 61y Zeg fifty 55 60 skd dad das aad SCS cas aas garden dkd dke ace sds dss cx css sds 240 Bie 61 and Azr Bz Bie Tug Azp 61 p Ya1 Ya1 TNG Agd A1a RNe Zeg Agd 65 70 75 80 grandfather das dds dss saa dkd akd skd skd ax dss Eai dds aas dss Szu 288 A1a Azr 61y A1a 61p Ya1 Mek Bie Bie Not A1a Tug 61y Azp A1a 61p 85 90 95 ace duck ska skd garden skd sas edd ssd yeah aka kde cas sed cx cx 336 TNG Azr Bie Bie 61p Bie ΗΪ3 Agd Rgo C1 and Not Suz Tug Rgo RNe RNe one hundred 105 BUT dds cx ace grandfather dkd yeah hell sack dad garden ace ax sed dkd asd sed 384 61y RNe TNG Ya1 Ya1 61and Zeg H13 61and 61p TNG Not Rgo Ya1 TNG Rgo 115 120 125 garden grandfather asd ax sss ddk sds grandfather skd ace dss ace aas cx aas sds 432 61p Ya1 TNG Not Rgo 61y Agd A1a Bie TNG A1a TNG Azp RNe Azp Agd 130 135 140 ace dad garden ax SCS cas edd ace sds dke dds yeah as skd sed garden 480 TNG 61and 61p Not Bie Tug Tgr TNG Agd Ya1 C1u 61and Tug Bie Rgo 61p 145 150 155 160 aas dds aac garden garden akd SCS grandfather edd skd aad hell garden dke garden dds 528 Azp C1u Azp 61p 61p Mek Bie A1a Agd Bie Bz Zeg 61p Ya1 61p 61y 165 170 175

- 73 015170

Edd aes 576 des Ya1 das dde FOOD FOOD FOOD sds aec Agde Not 185 Ead asd asd Tht 190 sss Rgo dad SHi Wah Bie Ya1 Zeg Tht Wah Tgr Not Azr 180 61 y grandfather yeah yes dds eat hell dss aaE CEC yes EEC grandfather sds dad eat grandfather 624 A1a 61and Azr 61y Bie Zeg A1a Azp Bie Azr Pb Aha Agd 61 and Bie Ya1 195 200 205 aad asd cEC das ssd sds grandfather eat sds ssd eat CEC ddd aas ddd cEC 672 Bz Tht Bie Azr Rgo Agd Ya1 Bie Agd Rgo Bie Bie 61y Azp 61y Bie 210 215 220 asa sdd garden CEC dde sas garden des Food 699 Tht Agd 61p Bie 61y NHC 61p Ya1 ♦

225 230 <210> 29 <211> 232 <212> RKT <213> ATCC53159 <220>

<221> WRITTEN <222> (0) ... (0) <223> РЕЕАЫЕ РУУЗАВСАРГББББББББББ ЕГГРГРЕГЕЕХП <400> 29

MeE Pb Azp Agd Agd Azr Bie Bie Not 61y A1a 61 y Suz Pb Aha Aha one 5 10 fifteen A1a 61y Aha Zeg Bie 61y Bie Bz Rgo NHC Agd Agd MeE Azr Bie Bie twenty 25 thirty SHU SHU YOU Bz Bie AZR Tht Bie MeE Rgo Bz Aha Pb 61y A1a Tgr 35 40 45 Bz Aha SHi Azr Tht 61y Zeg Bie Not A1a Rgo Aha Agd 61and 61 y Zeg fifty 55 60 Bie SHi Azr Bz Bie Tug Azp 61p Ya1 Wah Tht Agd A1a Pb Zeg Agd 65 70 75 80 Aha Azr SHU Aha SHP Wah MeE Bie Bie Not Aha Tug 61y Azp A1a SHP 85 90 95 Tht Azr Bie Bie SHP Bie NHC Agd Rgo 61and Not Suz Tug Rgo Pb Pb one hundred 105 110 SHU Pb Tht Wah Wah SHi Zeg NHC SHi C1p Tht Not Rgo Ya1 Tht Rgo 115 120 125 C1p Wah Tht Not Rgo SHU Agd A1a Bie Tht Aha Tht Azp Pb Azp Agd 130 135 140 Tht 61and 61p 11th Bie Tug Tgr Tht Agd Wah 61y C1 and Tug Yeah. Rgo C1p 145 150 155 160 Azp SHU Azp SHP C1p MeE Bie A1a Agd Bie Bz Zeg C1p Wah 61p SHU 165 170 175 Tgr Not Wah Azr C1u Ya1 Bie Wah Agd Not Zeg Tht Ya1 Tht Rgo C1 and 180 185 190

- 74 015170

Aba Sbi Azr Sbu Lee Zeg 195 A1a Azp Ley Azr Lye 200 A1a Agd Sbi 205 Ley Sbu Azp 220 Ley Wab Bf bb 210 Thr Agd Sbp 225 Azr Bie Rgo Agde Oub Aye Agde 215 Sbp uab Rgo Bie SBU Bie bbu Nbz 230 <210> thirty <211> 1395 <212> ΟΝΑ <213> Zrbpdotopaz: sp ATCC53159 <220> <221> C3 <222> (one).. . (1395) <223> And his

<400> 30

aed aas dss dee 48 DEE ssd aU MeE sds sds dds dds ssd sEC dss Aba sds Agd fifteen aU MeE OUB 5 Rgo MeE one Azp Aba wab Agd Agd SBU 10 SBU Rgo Bie sds yes ace dd eat sce dss sds des das DSE EAE das ace dss EEC 96 Agd Azr Tht OUB Bie Rgo Aba Agd OUB Azr Aba Tug Azr Tht Aba Pb twenty 25 thirty eat sce dss grandfather eat dad aec aec dad sdd ssd DEER Esd sss ace grandfather 144 Bie Rgo Aba Aba Bie Sbi Not Not Sbi Agd Rgo OUB Zeg Rgo Tht Aba 35 40 45 sdd CEE ace dss aad grandfather aU eat dss ddd eat grandfather aec ace dss dss 192 Agd Bie Tht Aba Bz OUB MeE Bie Aba SBU Bie Aba Not Tht Aba Aba fifty 55 60 Unit eat grandfather aec dds aad des yeah des des grandfather ssd asd garden ddd sdd 240 Tgr Bie Aba 11th SBU Bz OUB Sbi OUB OUB Aba Rgo Tht Sbp SBU Agd 65 70 75 80 aec grandfather ssd aec dds dad ace aad aec des garden Esd sss yeah Esd ddd 288 11th Aba Rgo I be SBU Sbi Tht Bz Not OUB Sbp Zeg Rgo Sbi Zeg SBU 85 90 95 aec des sds sds aec eat grandfather dds dad ddd garden aad des dss aad dds 336 Not OUB Agd Agd Not Bie OUB SBU Sbi SBU Sbp Bz OUB Aba Bz SBU one hundred 105 but garden grandfather eat aec asd cEC das ssd ace grandfather Esd Esd grandfather dad grandfather dsa 384 C1p OUB Bie Not Tht Bie Azr Rgo Tht OUB Zeg Zeg Aba Sbi Aba Aba 115 120 125

- 75 015170

grandfather garden 432 Bn ab aad dkd grandfather skd SKS ADE DSS Garden sks das dss dsa sds Agd aas Azp Bz Yb Bie Azr 140 Aba Aba Aba Bie Bie 135 Zeg Aba Bn 130 garden grandfather ax ax das grandfather skd das dds add dds cx sds cx dke grandfather 480 Bn Aba S S Azr Aba Bie Azr Sue Agd Sue Ръ.е Agd RNe Yb Aba 145 150 155 160 sec dss dss dss hell ssd dds yeah dkd grandfather asd sack sds dds SCS dss 528 Rgo Aba Aba Aba Zeg Rgo Sue SI Yb Aba you Hb Agd Sue Bie Aba 165 170 175 sds dss edd skd dds garden ax dad grandfather grandfather skd dss dds dds sds css 576 Agd Aba Agd Bie Sue Bn S SI Aba Aba Bie Aba Xiu Sue Agd Zeg 180 185 190 duck sds ddk dss dss dke sneaker dss grandfather dss dad grandfather garden dsa garden dkd 624 Azr Agd Sue A1a Aba Yb Zeg Aba Aba Aba SI Aba Bn Aba Bn Yb 195 200 205 edd aad SCS yeah garden sneaker skd ssd skd SCS yeah garden garden ax dss grandfather 672 Agd Bz Bie SI Bn Zeg Bie Rgo Bie Bie SI Bn Bn S Aba Aba 210 215 220 aas dad asd akd dss dss aad dds as dke sneaker aad skd sds dke d £ d 720 Azp SI Tht Mek Aba Aba Bz Sue Tug Yb Zeg Bz Bie Agd Yb Ya1 225 230 235 240 dad akd caustic sds garden skd ax dss dad edd garden das skd asd grandfather grandfather 768 SI Mek Agd Agd Bn Bie S Aba SI Agd Bn Azr Bie TNG A1a Aba 245 250 255 sds Dec asd SCS dss aah SCS dds garden garden sneaker skd hell dke css hell 816 Agd Aba Tht Bie Aba Bz Bie Sue Bn Bn Zeg Bie Zeg Yb Zeg Zeg 260 265 270 skd sneaker dss aad asd sds dad dad grandfather edd grandfather garden dd skd garden duck 864 Bie Zeg Aba Bz Tht Agd SI SI Aba Agd Aba Bn Yb Bie Bn Azr 275 280 285 skd dke aad grandfather garden das dad dkd caustic dss eating dds dad das dke dss 912 Bie Yb Bz Aba Bn Azr SI Yb Agd Aba Agd Sue SI Azr Yb Aba

290 295 300

- 76 015170

aad grandfather 960 aaS Azp sed sds Ley Agde hell Zeg 310 Sed sss sds daa ebd sds grandfather ssd Rgo dbd OUB hell Zeg 320 Bz 305 A1a Zeg Rye Agd Sbi Bie 315 Agd Aba dd ace dss Sed garden csd garden dbe sas ace yeah dds dds dbd dbd yeah 1008 C1u Tht OUB Zeg Sbp Bie Sbp OUB Nbz Tht Sbi SBU SBU OUB OUB Sbi 325 330 335 ddd dss aad ssd sbs ssc hell ebd dbb sss das aab dss sdd sbs dad 1056 61y Aba Bz Rgo Bie Bie Zeg Bie OUB Rgo Azr Azp Aba Agd Bie Sbi 340 345 350 dss dad dsd ASD dss das aas hell das abs dds Sbs dbe sas abs dds 1104 Aba (ZHi OUB MeC OUB Azr Azp Zeg Azr Not SBU Pb OUB Nbz Not SBU 355 360 365 abd ssd dSa aad grandfather aad ebd garden dss STS sss Sub ace sds bass dds 1152 Meb Rgo OUB Bz OUB Bz Bie Sbp Aba Pb Rgo Tug Tht Agd Tug SBU 370 375 380 asd ACC sss dds asd dad grandfather dds abs hell sss dad grandfather dbd garden abd 1200 MeC Not Rgo SBU Tht OUB Aba SBU Not Zeg Rgo Sbi Aba OUB Sbp Meb 385 390 395 400 aad dad aas garden ssd ssd dbe bass aad grandfather sdd abs grandfather ebd grandfather eating 1248 Bz Obi Azp Sbp Rgo Rgo OUB Tug Bz Aba Agd Not Aba Bie Aba Agd 405 410 415 ddd CaS dsd csd dss caS dds dsa garden dbd ssd ebd sdd ssd ddd abd 1296 61y tug OUB Bie Aba Nbz SBU Aba Sbp OUB Rgo Bie Agd Rgo SBU Meb 420 425 430 sbs grandfather hell grandfather das ACC dbe ace dds aad food ace ebd bbe hell women 1344 Bie Aba Zeg Aba Azr Not OUB Tht SBU Bz Agd Bj Bie Pb Zeg Tug 435 440 445 csd dsd ddd sss dsd sss dad asd ddd adb das grandfather ebd sas dad sdd 1392 Bie OUB SBU Rgo OUB Bie Sbi TNG SBU Zeg Azr Aba Bie Nbz Sbi Agd 450 455 460

food

1395 *

<210> 31

- 77 015170 <211> 464 <212> RKT <213> Sp. ATCC53159 <220>

<221> RETURN <222> (0). . . (0) <223> ρυΕβΕίνβ ес Ы р р р го го <<<<400> 31

MeE Azp A1a one Wa1 Wa1 5 Rgo MeE Agd Agd 61u CXU 10 Rgo lei A1a Agd fifteen MeE Agd Azr TNG Ya1 Bie Rgo A1a Agd Ya1 Azr A1a Tug Azr YOU A1a Pb twenty 25 thirty Bie Rgo A1a A1a Bie 61and Not Not C1 and Agd Rgo Ya1 Zeg Rgo Tht A1a 35 40 45 Agd Bie Tht A1a Bz Ya1 MeE Bie A1a 61y Bie A1a 11th Tht A1a A1a fifty 55 60 Tgr Bie A1a Not 61y Bz Ya1 C1 and Ya1 Ya1 A1a Rgo Tht C1p C1u Agd 65 70 75 80 Not A1a Rgo Not C1u 61and TNG Bz Not Ya1 61p Zeg Rgo 61and Zeg C1u 85 90 95 Not Ya1 Agd Agd Not Bie Ya1 61y C1 and C1u 61p Bz νβΐ A1a Bz 61y one hundred 105 110 61p Ya1 Bie Not Tht Bie Azr Rgo Tht Ya1 Zeg Zeg A1a C1 and A1a A1a 115 120 125 C1p A1a Bz Ya1 A1a Bie Bie Zeg A1a 61p Bie Azr A1a A1a Agd Azp 130 135 140 ζ- »Ί Ί T ί _L T 1 L Azr l Ί ~ _t Azr ,, G * Ί ·. r> k ~ A1a υχΠ L1.Y hehe 2-1X0 and & and Ch7hu 2 * 1 X Y them at GMO 21X _U Σ 14 ^ 2 V C1X 145 150 155 160 Rgo A1a A1a A1a Zeg Rgo 61y 61and Ya1 A1a Tht H1z Agd 61y Bie A1a 165 170 175 Agd A1a Agd Bie <31u C1p Not 61and A1a A1a Bie A1a C1u C1u Agd Zeg 180 185 190 Azr Agd C1u A1a A1a Ya1 Zeg A1a A1a A1a C1 and A1a C1p A1a 31p Ya1 195 200 205 Agd Bz Bie C1 and 61p Zeg Bie Rgo Bie Bie C1 and 61p 61p Not A1a A1a 210 215 220 Azp C1 and Tht MeE A1a A1a Bz 61y Tug Ya1 Zeg Bz Bie Agd Ya1 Ya1 225 230 235 240 61and MeE Agd Agd C1p Bie Not A1a C1 and Agd C1p Azr Bie Tht A1a A1a 245 250 255 Agd A1a Tht Bie A1a Bz Bie C1u 61 p C1p Zeg Bie Zeg Ya1 Zeg Zeg 260 265 270 Bie Zeg A1a Bz Tht Agd C1 and 61and A1a Agd A1a 61p Ya1 Bie 61 p Azr 275 280 285 Bie Ya1 Bz A1a C1p Azr 61and Ya1 Agd A1a Agd C1u 61and Azr Ya1 A1a 290 295 300 Bz A1a Azp Bie Agd Zeg Zeg Pb Agd 61and Bie Agd A1a Rgo Ya1 Zeg 305 310 315 320 31u YOU Ya1 Zeg b1p Bie C1p Ya1 H1z Tht C1 and b1u boo Ya1 Ya1 C1 and 325 330 335 C1u A1a Bz Rgo Bie Bie Zeg Bie Ya1 Rgo Azr Azp A1a Agd Bie 61and 340 345 350 A1a C1 and Ya1 MeE Ya1 Azr Azp Zeg Azr Not 61y Pb Ya1 ΗΪ3 Not 61y 355 360 365 MeE Rgo Ya1 Bz Ya1 Bz Bie 61p A1a Pb Rgo Tug Tht Agd Tug 61y

- 78 015170

370 375 380 MeE Bb Rgo Sue Tkg Yb Aba Sue Bb Zeg Rgo SI Aba Yb Bn MeE 385 390 395 400 Bz SI Azp Bn Rgo Rgo Yb Tug Bz Aba Agd Bb Aba Bie Aba Agd 405 4b0 415 C1u Tug Yb Bie Aba Nhz Sue Aba Bn Yb Rgo Bie Agd Rgo C1u MeE 420 425 430 Bie Aba Zeg Aba Azr Not Yb Tkg 61 y Bz Agd Tkg Bie Rke Zeg Tug 435 440 445 Bie Yb Sue Rgo Yb Bie SI Tkg Sue Zeg Azr Aba Bie Nhz SI Agd 450 455 460

<210> 32 <211> 2187 <212> ϋΝΑ <213> ATCC53159 <220>

<221> C05 <222> (1). . . (2187) <223> aEgV <400> 32 akd asa sds ss das daa aed garden dss ace skd garden adé grandfather cEC grandfather dss 48

Mek Tkg Agd Azr SI Mek Sbn Aa Tkg Lye Sz Zeg Aa Lye Aya Aa

5 10 15

sae 96 nhz ddd grandfather dad sds dad dad dad eat Bie sds Agd yeah SI Ess dda eat Bie dd Yb Sue Aba Aba twenty SI Aba SI 25 SI Agd Zeg Sue thirty grandfather Food Eating eat eat cEC dds grandfather sas aas aec dss aec asd sss yeah 144 Aba Bie Zeg Bie Bie Bie Sue Aba Nhz Azp Bb Aba Not Tkg Rgo C1 and

40 45

garden sed sds sas grandfather eat dds sae dss dad grandfather dsa hell dss das das 192 C1p Bie Agd H13 Aba Bie Sue Hb A1a SI Aba Aba Zeg A1a Azr Aar fifty 55 60

sEd aEc cEC sEd Еи еи dss Aba aad Bz 70 sds garden garden dds ded sds dss aad dss des Yb 80 240 Bie 65 1b Sue Yb 75 Agd Aba Bz Aba Agd Bn bn dad grandfather sed sds dds dda cEC dss sds garden sed eat sss grandfather aec dss 288 SI Yb Rgo Agd Sue Sue Bie Aba Agd Bn Rgo Bie Rgo Aba Bb Aba

90 95 das ddd dcc dda dds edd eec ded aec dds dds ced ase daa sae dds 336

Azr Sju Rgo Si S1u Tgr Rke Ua1 11e S1u Sbu Lei Tkg S1i Nhz Sju

100 105 110

- 79 015170

DEED aEc ACC T1e 115 garden sds ssd dds saS dss ssd yeah garden des das sdd Agd das Azr 384 Yb Not Bn Agd Rgo Sue Hb 120 Yb 125 Azr Aba Rgo SI Bn grandfather csd das grandfather ACC edd Sss dds grandfather eat grandfather eat ssc aes ace sds 432 Aba Bie Azr Aba Not Tgr Zeg Sue Aba Bie Yb Bie Bie TNG TNG Agd 130 135 140 grandfather grandfather grandfather dda sdd ssd eat sdd SSc dds cEC Sss edd SSc ace grandfather 480 Aba Yb Aba Sue Agd Rgo Bie Agd RNe Sue Bie Zeg Tgr RNe TNG Aba 145 150 155 160 garden SSc edd sds CaE sds asd eat SSc ssc dad grandfather ssc dds ACC ace 528 Bn RNe Agd Agd Tug Agd TNG Bie RNe Bie SI Yb Bie Sue Bb TNG 165 170 175 ssc grandfather ssc aas eat ssc dds ssc dss grandfather ssd eat sed EUs garden hell 576 Bie Aba Bie Azp Bie Bie Sue Bie Aba Aba RGO Bie Bie RNe Bn Zeg 180 185 190 grandfather ACC das aad grandfather sed ace sas aas hell ASD hell asd eat hell grandfather 624 Yb Not Azr Bz Yb Bie Not Hb Azp Zeg Month Zeg TNG Bie Zeg Yb 195 200 205 ssc dss CEC dss SSc eat grandfather dss Ssd grandfather sdd yeah grandfather grandfather ssc dds 672 Bie Aba RNe Aba RNe Bie Aba Yb Zeg Yb Tgr SI Yb Aba Bie 61y 210 215 220 edd ACC sds ace sds ssd SSc ace dad asd asd garden aad ACC das des 720 Tgr 11th Agd TNG Agd Bie RNe TNG SI TNG TNG Bn Bz Not Azr Yb 225 230 235 240 dad pso csd dde dss sdd eat EEC sas sas eat eat grandfather csd ssd ssc dss 1 V V SI Bie Xiu Aba Agd Bie RNe Hb Hb Bie Bie Aba Bie Rgo Bie Aba 245 250 255 CaS SSc dad aad sds sds grandfather dds das ace des aes sds des sds garden 816 Tug RNe SI Bz Agd Agd Yb Sue Azr TNG Yb TNG Agd Yb Agd Bn 260 265 270 ssc dad asd ACC sds yeah SSc CCC ace hell dss Ssd sed asd grandfather ASD 864 Bie SI TNG Not Agd SI RNe Bie TNG Zeg Aba Zeg Bie TNG Yb MeC

275 280 285

- 80 015170

dkd das ssd Rgo skd Bie cx Rke ace kx dkd Ya1 KKS SKS DSS Grandfather Akd skd Bie cx Rke cas Tug 912 Tkg Rke 295 Wa1 Azr Rke Bie A1a A1a Mek 300 290 ssd sneakers 960 akd SCS sneaker dds ax dkd SCS dkd sneaker skd ax dss as grandfather Zeg Rgo 305 Mek Bie Zeg 61y 310 Not Ya1 Bie Ya1 Zeg 315 Bie Not A1a Tug A1a 320 ax dka 1008 sneaker cx hell dks dss ddd ssd SCS sds grandfather edd dkd dad das 11th Wa1 Zeg Rke Zeg 325 Ya1 A1a 61y Rgo Bie 330 Agd A1a Agd Ya1 61and. 335 Azr aad kks 1056 dad aad hell css dss hell aas grandfather skd SCS dks dad hell dks Bk rke 61 and Bz 340 Zeg Zeg A1a Zeg Azp 345 A1a Bie Bie Ya1 61and 350 Zeg Ya1 sneaker dds 1104 ax sas asd ax aad grandfather ace grandfather dks dad ssd sas cdd garden Zeg 61u 11th 355 H13 Tkg 11th Bz A1a 360 Tkg A1a Ya1 61and Rgo 365 Ηί3 Tgr 61p aak sds 1152 cdd dad sds garden SCS dss dss sack ace dss grandfather sneaker garden edd Azp Agde 370 Tgr 61and Agd 61p Bie 375 A1a A1a H1z Tkg A1a 380 A1a Zeg 61p Agd skd ax 1200 aac ace dss aas ace dds hell garden grandfather ax dad skd ax sneaker Ley 11th 385 Azp Tkg A1a Azp 390 Tkg 61y Zeg C1p A1a 395 11th 61and Bie Not Zeg 400 aad skd 1248 hell cx grandfather grandfather ax skd cx cx dds dss aad grandfather dkd ax Bj b Zeg Rke A1a 405 A1a Not Bie Rke Rke 410 C1u A1a Bz A1a Ya1 415 Not dds dds 1296 grandfather akd hell dka dds grandfather skd dkd grandfather cx aas akd cx dss 61u 61u A1a Mek 420 Zeg Ya1 61y A1a Bie 425 Ya1 A1a Rke Azp Mek 430 Rke A1a garden sds 1344 dkd css ddd ssd dkd ax sds akd grandfather garden skd cdd garden duck 61p agd Ya1 435 Zeg C1u Rgo Ya1 Not 440 Agd Mek A1a 61p Bie 445 Tgr 61l Azr kks garden 1392 garden dkd sds ax sneaker dks dad edd skd dds das dkd SCS aas Rke 61p 450 C1p Ya1 Agd Not Zeg 455 Ya1 61and Agd Bie 61y 460 Azr Ya1 Bie Azp sack ssd grandfather yeah ssd sds ssd dss sneaker grandfather grandfather asd skd ssd dkd skd

1440

- 81 015170

H1z 4 65 Rgo Ya1 61and Rgo Agde 470 Rgo A1a Zeg A1a A1a Th2 475 Bie Rgo Ya1 Bie 480 sds 1486 ddk grandfather acc sds ys dad aac dke hell cx sds as dss dad das Agd C1u A1a Not Agd 485 Pb 61and Azp Ya1 5eg 490 Pb Agd Tug A1a 61and 495 Azr garden ssd ssd dkd skd hell give ax asd SCS das acc ssd grandfather dde ace

1536

C1p Rgo Rgo Wa1 500 Bie Zeg Azr 11th Thr Lei Azr 11th Rgo A1a 61u Tht 505 510 sneaker SCS dde ax dke ddk sneaker sneaker dde sneaker dde aad sneaker asd skd dss 1584 run Bie C1u Not Ya1 61y Zeg Zeg 61y Zeg C1u Bz Zeg Tht Bie A1a 515 520 525 aad skd SCS garden edd SCS aas led ssd aac SCS dde sds dkd skd dke

1632

Bz Bie 530 Bie 61p agd Bie Azp Lye 535 Rgo.Azp Ley 61u 540 Agde Wa1 Ley Wa1 das dad dke das d ^ d grandfather garden SCS duck sss dss cdd skd caustic sds garden 1680 Azr 61and Ya1 Azr Ya1 A1a 61p Bie Azr Rgo A1a Tgr Bie Agd Agd 61p 545 550 555 560 ax dde dke dkd skd garden dad aac skd skd X hell sds sneaker ax eating

1728

11e 61u Wa1 Ya1 Ley 61p 565 61 and Azp Bie Bie 570 Pb Zeg Agd Zeg Not 575 Agd dad aas ax grandfather SCS Ess aas sss dss akd sed X dad aac dke dke 1776 61and Azp Not A1a Bie Zeg Azp Rgo A1a Mek Rgo Pb 61and Azp Ya1 Ya1 580 585 590 grandfather 1824 grandfather grandfather asd skd dss dde grandfather sack duck X ax skd sds garden ssd A1a A1a A1a Tht Bie A1a 61y A1a ΗΪ3 Azr Pb Not Bie Agd C1p Rgo 595 600 605 sds 1872 dde as das ace dad ax dke dad sds dde dke aac SCS css dde Agd 61 y Tug Azr Tht 61and Not Ya1 61and Agd 61 y Ya1 Azp Bie Zeg 61y 610 615 620 dde 1920 garden sds garden edd SCS dss ax dss sds grandfather SCS dke dde aac ssd 61y 61p Agd 61p Agd Bie A1a Not A1a Agd A1a Bie Ya1 61y Azp Rgo 625 630 635 640 sds 1968 ax skd dkd EEC das yeah grandfather ace sneaker grandfather SCS duck dss dad hell Agd Not Bie Ya1 Pb Azr 61and A1a Tht Zeg A1a Bie Azr A1a 61and Zeg 645 650 655

- 82 015170

dad dad skd ax garden aas aas skd sds dss ax sneaker dss dds sds asd 2016 SI SI Bie S Bn Azp Azp Bie Agd Aba S Zeg A13 Xiu Agd Tht 660 665 670 sks dkd dkd ax grandfather sack sds skd hell grandfather d ^ d sds hell kde das edd 2064 Bw bb Yb S Aba Hb Agd Bie Zeg Aba Yb Agd 5eg Suz Azr Agd 675 680 685 ax ax asd SCS yeah garden dds sds ax dke dad hell dds food sas das 2112 Wow Wow TNG Bie SI Bn Sue Agd Bb Yb SI Zeg Sue Agd Hb Azr 690 695 700 yeah qcd ccd sds Cd dds dds sds as dss das skd sas sds sds garden 2160 SI Bie Agd Bie Sue Sue Agd Tug Aba Azr Bie Hb Agd Agd Bn 705 710 715 720 dds dds as ddd dad acc dss dsa kda 2187 Sue Sue Tug Sue SI S Aba Aba *

725 <210> 33 <211> 728 <212> CTD ATCC53159 <220>

<221> RETURN <222> (0). . . (0)

Mek one TNG Agd Azr SI 5 Mek Ala Bie 10 Bn Zeg Aba A1a fifteen Aba Hb Sue Aba Aba SI Agd SI Aba SI Bie Agd SI Zeg Sue Bie Yb twenty 25 thirty Aba Bie Zeg Bie Bie Bie Sue Aba Hb Azp S Aba S TNG Rgo SI 35 40 ⁴⁵ Bn Bie Agd Hb A1a Bie Sue Hb Aba SI Aba Aba Zeg Aba Azr Azr fifty 55 60 Bie S Bie Bie Aba Bz Agd Bn Bn C1u Yb Agd Aba Bz Aba Yb 65 70 75 80 SI Yb Rgo Agd Sue Sue Bie Aba Agd Bn Rgo Bie Rgo Aba Bb Aba 85 90 95 Azr Sue Rgo SI Sue Tgr RNe Yb S Sue Sue Bie Tht SI Hb Sue one hundred 105 yo Yb S S Bn Agd Rgo Sue Hb Aba Rgo SI Bn Yb Azr Agd Azr 115 120 125 Aba Bie Azr Aba Bb Tgr Zeg Sue Aba Bie Yb Bie Bie TNG TNG Agd 130 135 140 Aba Yb Aba Sue Agd Rgo Bie Agd RNe C1u Bie Zeg Tgr RNe TNG Aba

- 83 015170

145 150 155 160 61η RNe Agd Agd Tug Agd. TNG Bie RNe Bie 61and Ya1 Bie 61y Not TNG 165 170 175 Bie A1a Bie Azp Bie Bie 61y Bie A1a A1a Rgo Bie Bie RNe 61p Zeg 180 185 190 Ya1 Not Azr Bz Ya1 Bie Not ΗΪ3 Azp Zeg Mek Zeg TNG Bie Zeg Ya1 195 200 205 Bie A1a RNe A1a RNe Bie A1a Ya1 Zeg Ya1 Tgr 61and Ya1 A1a Bie 61y 210 215 220 Tgr Not Agd TNG Agd Bie RNe TNG 61and TNG TNG 61p Bz Not Azr Ya1 225 230 235 240 61and Bie 61y A1a Agd Bie RNe ΗΪ3 Ηίδ Bie Bie A1a Bie Rgo Bie A1a 245 250 255 Tug RNe C1 and Bz Agd Agd Ya1 61y Azr TNG Ya1 TNG Agd Ya1 Agd 61p 260 265 270 Bie 61and TNG Not Agd 61and RNe Bie TNG Zeg A1a Zeg Bie TNG Ya1 Mek 275 280 285 Ya1 Azr Rgo Bie RNe TNG RNe Ya1 Pb Bie A1a A1a Mek Bie RNe Tug 290 295 300 Zeg Rgo Mek Bie Zeg 61y Not Ya1 Bie Ya1 Zeg Bie Not A1a Tug A1a 305 310 315 320 Not Ya1 Zeg RNe Zeg Ya1 A1a 61y Rgo Bie Agd A1a Agd Ya1 61and Azr 325 330 335 Bz RNe 61and Bz Zeg Zeg A1a Zeg Azp A1a Bie Bie Ya1 61and Zeg Ya1 340 345 350 Zeg C1u Not ΗΪ5 TNG Not Bz A1a TNG A1a Ya1 61and Rgo H1z Tgr 61p 355 360 365 Azp Agd Tgr 61and Agd C1p Bie A1a A1a H1z TNG A1a A1a Zeg 61p Agd 370 375 380 Bie Not Azp TNG A1a Azp TNG 61y Zeg 61p A1a Not 61and Bie Not Zeg 385 390 395 400 Bz Bie Zeg RNe A1a A1a Not Bie RNe RNe 61y A1a Bz A1a Ya1 Not 405 410 415 C1u C1u A1a Mek Zeg Ya1 61y A1a Bie Ya1 A1a Pb Azp Mek RNe A1a 420 425 430 61p Agd Ya1 Zeg C1u Rgo Ya1 Not Agd Mek A1a 61p Bie Tgr 61p Azr 435 440 445 Pb 61p 61p Ya1 Agd Not Zeg Ya1 61and Agd Bie <31u Azr Ya1 Bie Azp 450 455 460 ΗΪ3 Rgo Ya1 61and Rgo Agd Rgo A1a Zeg A1a A1a TNG Bie Rgo Ya1 Bie 465 470 475 480 Agd 61y A1a Not Agd RNe 61and Azp Ya1 Zeg RNe Agd Tug A1a 61and Azr 485 490 495 61p Rgo Rgo Ya1 Bie Zeg Azr Not TNG Bie Azr Not Rgo A1a 61y TNG 500 505 510 Zeg Bie 61y Not Ya1 61y Zeg Zeg 61y Zeg C1u Bz Zeg TNG Bie A1a 515 520 525 Bz Bie Bie 61p Agd Bie Azp Bie Rgo Azp Bie 61 y Agd Ya1 Bie Ya1 530 535 540 Azr 61and Ya1 Azr Ya1 A1a 61p Bie Azr Rgo A1a Tgr Bie Agd Agd 61p 545 550 555 560 Not 61y Ya1 Ya1 Bie 61p 61 and Azp Bie Bie Pb Zeg Agd Zeg Not Agd 565 570 575 61and Azp 11th A1a Bie Zeg Azp Rgo A1a Mek Rgo RNe 61and Azp Ya1 Ya1 580 585 590 A1a A1a A1a TNG Bie A1a 61y A1a H1z Azr rne Not Bie Agd 61p Rgo 595 600 605

- 84 015170

Agd Sbu Tug Azr YOU SBI BBE WAB 615 SBI AGD SBU Oub Azp 620 Bie Zeg SBU 610 SBU Sbp Agd Sbp Agd Bie Aba bbe Aba Agd Aba Bie OUB SBU Azp Rgo 625 630 635 640 Agd 11th Bie OUB Pb Azr Sbi Aba YOU Zeg Aba Bie Azr Aba Sbi Zeg 645 650 655 Sbi Sbi Bie bbe Sbp Azp Azp Bie Agd Aba bbe Zeg Aba SBU Agd YOU 660 665 670 Bie OUB OUB 11th Aba Nbz Agd Bie Zeg Aba OUB Agd Zeg Suz Azr Agd 675 680 685 Not bbe Tht Bie Sbi Sbp SBU Agd bbe OUB Sbi Zeg SBU Agd Nbz Azr 690 695 700 Sbi Bie Bie Agd Bie SBU SBU Agd Tug Aba Azr Bie Nbz Agd Agd Sbp 705 710 715 720 SBU SBU Tug SBU Sbi bbe Aba Aba

725 <210> 34 <211> 1413 <212> ΟΝΑ <213> Sp. ATCC53159 <220>

<221> SRZ <222> (1). . . (1413) <223> yrzV <400> 34

aU 48 MeE one aas Azp DSE Aba EEC Pb yeah dsa garden sds dss EEE dad dad saa SEE sdd Agd fifteen grandfather Aba Pb 10 Sbi Sbi Sbp Bie Sbi 5 Aba Sbp Agd Aba sae Ess sdd DEER asd ssa ECE dss DSE sss dkd Unit cde sds Esd asd 96 Nbz Zeg Agd OUB Tht Rgo Zeg Aba Aba Rgo OUB Tgr Agd Agd Zeg YOU twenty 25 thirty SED sdd aU des cEC EAE ace dad Food eat eat eat das hell cEC Esd 144 Bie Agd MeE OUB Bie Tug Tht Sbi Bie Bie Bie Bie Azr Zeg Bie Zeg 35 40 45 aec eat dss dda EEC sas des grandfather grandfather dds asd sds das dds aas Unit 192 I be Bie Aba SBU Pb Nbz OUB Aba Aba SBU YOU Agd Azr SBU Azp Tgr fifty 55 60 eat Esd eat grandfather dds aec aas des dds des EEC eat eat ssd aec DSE 240 Bie Zeg Bie Aba SBU Not Azp OUB SBU OUB Pb Bie Bie Rgo Not Aba 65 70 75 80 cEC dds ace grandfather cEC dsa hell dds ace Eas Esd eat aas Food eat sds 288 Bie <31U Thr Aba Bie Aba Zeg SBU Thr Tug Zeg Bie Azp Suz Bie Agd 85 90 95

- 85 015170

Bac 336 Tug ssd des Rgo Wa1 adde dds grandfather aad hell aes her eat Zeg dsa her eee eee eat Zeg Ya1 Bj Zeg Not 105 Pb Zeg one hundred 61U A1a Pb Pb but Pb abc eee des des sed ses dds hell eae cd sed asd dss dad sed ssd 384 Not Pb Ya1 Ya1 Bj Bj 61y Zeg Tug Bj Bie Thr A1a C1 and Bj Rgo 115 120 125 cd ess sds grandfather garden sed grandfather dad dds grandfather aes ses Bcd sed dc cbc 432 Bj Zeg Agd Ya1 C1p Bj A1a 61and 61y A1a 11th Bie Zeg Bj Ya1 Bj 130 135 140 sed aed grandfather dds sds sed ad eee sds sds sas dc sds grandfather more ace 480 Bj Mee Ya1 e1u Agd Bj Mee Pb Agd Agd Hb Ya1 Agd A1a Ya1 Tht 145 150 155 160 dds dds add sed ses das yeah sed des aes abc das dds des eat ses 528 C1u C1u Agd Bj Bie Azr 61and Bie Ya1 Not Not Azr C1u Ya1 Zeg Bie 165 170 175 das des grandfather dds aah grandfather des grandfather cbc das grandfather edd abs aes aah cbc 576 Azr Ya1 A1a C1u Azp A1a Ya1 A1a Bj Azr A1a Agd Not 11th Azp Bie 180 185 190 eat ssd aah ssd sds give ssd saa aed sed sae sds sed dds ace ace 624 Zeg Rgo Azp Rgo Agd Azr Rgo 61p Me Bie Hb Agd Bie b1u Thr Thr 195 200 205 grandfather aes ddd eee das edd grandfather aes des dss eating ace aad dad sae sds 672 Ya1 Not C1u rye Azr Agd Ya1 Not Ya1 A1a Suz TNG Bz 61and ΗΪ3 Agd 210 215 220 grandfather des edd grandfather sed sed ses aad dds aed aah aes aad dds dad abc 720 A1a Ya1 Tgr A1a Bj Bj Bj Bz C1u meer Azp Not Bj 61y <31and Not 225 230 235 240 ses des sss garden eee aah grandfather sed dds grandfather abc dds grandfather das dss eee 768 Bie Wa.1 Rgo C1p RNe Azp A1a Bie 61y A1a Not C1u Ya1 Azr A1a Pb 245 250 255 das ddd aad give asd sed des dc eat garden dds ssd ses aah aed sss 816 Azr C1u Bj Azr Tht Bie Ya1 Ya1 Zeg C1p 61y Rgo Bie Azp Mee Rgo 260 265 270

- 86 015170

aas sds 864 Azp Agde grandfather aad aad sds grandfather cEC yes cEC grandfather aece ace deaa ssd dss A1a 275 Bz Bz Agd A1a Ley Azr 280 Bie A1a Not Tht 285 Ya1 Rgo A1a dsd cEC grandfather eat grandfather ssd eat aU aec eat dsd grandfather aec eat aec aad 912 Ya1 Bie A1a Bie A1a Rgo Bie MeE 11th Bie Ya1 A1a Not Bie Not Bz 290 295 300 eat dad hell ssd dds ssd grandfather Food EEC grandfather garden das sds des dds sds 960 Bie 61and Zeg Rgo 61y Rgo Ya1 Bie Pb A1a 61p Azr Agd Ya1 b1u Agd 305 310 315 320 dds aas edd eat Bb aad aec aU aad EEC sds Sed aU eating yeah asd

1008

31u Azp Agd Bie Pb Bz Not MeE Bz Pb Agd Zeg MeE Agd Ya1 Tht 325 330 335 Eat Food das grandfather aas dds aas des Eating dss hell sds das das Dan sds 1056 Bie suz Azr A1a Azp 61U Azp Ya1 Zeg A1a Zeg Agd Azr Azr Azr Agd 340 345 350 aes ace aad bottom dds eating Bb a £ s sds aad ace hell aec das yeah eat

1104

Do not bk Ya1 C1u Agd Pb 11th 3 60 Agd bt Zeg Not Oygk Azr <31and Bie OSS ssd garden 1152 eat eat aas DVD eat sds dds das aU hell two two dds ssd Рgo 61п 370 Bie Bie Azp Ya1 Bie 375 Agd C1u Azr MeE Zeg 380 Ya1 Ya1 C1u Rgo sdd ssd 1200 sae grandfather eat dds Eating sds dss dss yes sas eat EEC Unit yeah Agde Rgo 385 ΗΪ3 A1a Bie C1u 390 Zeg Agd A1a A1a Azr 395 H1z Bie Pb Tgr C1 and 400 aec das 1248 dad sds You airborne sas sds sas asd cEC aad sed dds aU ace 11th Azr C1 and Agd Tug 405 Tgr ΗΪ3 Agd H1z Tht 410 Bie Bz Rgo C1u MeE 415 Tht dde sEd 1296 dss garden grandfather sds dd £ EEC sds ddd grandfather ace yes eating sds two C1 y A1a C1p 420 Ya1 Agd C1u Pb Agd 425 C1u A1a Tht Azr Agd 430 Agd Ya1 das ed 1344 ace aas sdd cEC garden dsa das aU yeah EAE aec das dda Bdd Azr Lye Tht 435 Azp Agd Bie C1p A1a 440 Azr MeE C1 and Tug Not 445 Azr C1u Tgr yes aeS Unit sds yes aec asd aec cd EEC aad asd eat sdd dd aec

1392

- 87 015170

Azr Ne Tgr Agd Azr Ne Tg Not Yee Rye bw bie agd wa1 not 460 450 455 drd sak Eating aas dsa eee food 1413 Wa1 Nhz Zeg AZP A1a Pb

465 470 <210> 35 <211> 470 <212> RKT <213> ATCC53159 <220>

<221> PERTURBE <222> (0) ... (0) <223> d1 isosu

Mee Azp A1a Pb 61and A1a 61p Agd A1a Pb C1 and C1 and C1p Bie Agd A1a one 5 10 fifteen H13 Zeg Agd Ya1 TNG Rgo Zeg A1a A1a Rgo Ya1 Tgr Agd Agd Zeg Tht twenty 25 thirty Bie Agd Mee Ya1 Bie Tug Tht 61and Bie Bie Bie Bie Azr Zeg Bie Zeg 35 40 45 Not Bie A1a 61y Pb Nhz Ya1 A1a A1a C1u YOU Agd Azr C1u Azp Tgr fifty 55 60 Bie Zeg Bie A1a 61y 11th Azp Ya1 61U Ya1 Pb Bie Bie Rgo Not A1a 65 70 75 80 Bie 61y Tht A1a Bie A1a Zeg C1u Thr Tug Zeg Bie Azp Suz Bie Agd 85 90 95 Tug Rgo Ya1 Zeg C1u Ya1 Bz Zeg Not Pb Zeg A1a Pb Pb Pb Zeg one hundred 105 110 Not Pb Ya1 Ya1 Bie Bie 61y Zeg Tug Bie Bie Thr A1a C1 and Bie Rgo 115 120 125 Bie Zeg Agd Ya1 61p Bie A1a 61and 61y A1a 11th Bie Zeg Bie Ya1 Bie 130 135 140 Bie Mek Ya1 B1u Agd Bie Mee Pb Agd Agd Nhz Ya1 Agd A1a Ya1 Thr 145 150 155 160 C1u C1u Agd Bie Bie Azr 61and Bie Ya1 Not Not Azr C1u Ya1 Zeg Bie 165 170 175 Azr Ya1 A1a 61y Azp A1a Ya1 A1a Bie Azr A1a Agd Not Not Azp Bie 180 185 190 Zeg Rgo Azp Rgo Agd Azr Rgo C1p Mee Bie HX5 Agd Bie C1u Thr Thr 195 200 205 Ya1 Not <51y Pb Azr Agd Ya1 Not Ya1 A1a Suz Thr Bz C1 and Nhz Agd 210 215 220 A1a Ya1 Tgr A1a Bie Bie Bie Bz B1u Mee Azp Not Bz C1u 61and Not 225 230 235 240 Bie Ya1 Rgo C1p Pb Azp A1a Bie C1u A1a Not 61y Ya1 Azr A1a Pb 245 250 255 Azr 61y Bz Azr Thr Bie Ya1 Ya1 Zeg 61p C1u Rgo Bie Azp MeE Rgo 260 265 270 Azp Agd A1a Bz Bz Agd A1a Bie Azr Bie A1a Not Thr Ya1 Rgo A1a 275 280 285 Ya1 Bie A1a Bie A1a Rgo Bie Mee Not Bie Ya1 A1a 11th Bie Not Bz

- 88 015170

290 295 300 Bie SI Zeg Rgo Sue Rgo Yb Bie RNe Aba Bn Azr Agd Yb Sue Agd 305 310 315 320 C1u Azp Agd Bie RNe Bz Ye Me Bz RNe Agd Zeg Me Agd Yb Tht 325 330 335 Bie Suz Azr Aba Azp Sue Azp Yb Zeg Aba Zeg Agd Azr Azr Azr Agd 340 345 350 Not Tht Bz Yb Xiu Agd RNe Not Agd Bz Tht Zeg Not Azr SI Bie 355 360 365 Rgo Bn Bie Bie Azp Yb Bie Agd Sue Azr Me Zeg Yb Yb Sue Rgo 370 375 380 Agd Rgo Hb Aba Bie Sue Zeg Agd Aba Aba Azr Hb Bie RNe Tgr SI 385 390 395 400 Ye Azr SI Agd Tug Tgr Hb Agd Hb TNG Bie Bz Rgo Ce Me Thr 405 410 415 C1u Bie A1a Bn Yb Agd Ce RNe Agd Ce Aba TNG Azr Agd Agd Yb 420 425 430 Azr Bie TNG Azp Agd Bie Bn Aba Azr Me SI Tug Not Azr Ce Tgr 435 440 445 Azr Ye Tgr Agd Azr I b TNG 1b Bie RNe Bz TNG Bie Agd Yb Pe 450 455 4 60 Yb H13 Zeg Azp Aba RNe

465 470 <210> 36 <211> 879 <212> ΟΝΑ <213> ATCC53159

<220> <221> C3 <222> (1) ... <223> gta . (879) <400> 36 ab aad dds abc abc bb grandfather ddd dds hell ddd asd sds cd Bac sss 48 Me Bz Ce Ye Ye Bie Aba Ce Ce Zeg Ce Thr Agd Bie Tug Rgo one 5 B B5 dsa asd ba Bcd abc Bcd aad garden cd bb sss dc Ba das aad ssd 96 Aba Thr Bie Zeg Ye Zeg Bz Bn Bie Bie Rgo Yb Tug Azr Bz Rgo twenty 25 thirty ab abc Bc Ba ssd cd Bcd dd cd ab Cbc ace dds abc edd das 144 Me Ye Pb Tug Rgo Bie Zeg Yb Bie Me Bie Thr Ce 1b Agd Azr 35 40 45 abc cd ab abc Bss ace ssd sds das cd ssd ab Bc garden grandfather cd 192 Ye Bie Ye Ye Zeg Thr Rgo Agd Azr Bie Rgo Me Pb Bn Aba Bie fifty 55 60 cd dds das dds Bcd dss Bc dds abc aas cbc hell Ba dss dad garden 240

- 89 015170

Ley 61u Azr C1u 65 Zeg A1a 70 Rke 61u Ne Azp Ley Zeg Tug A1a 75 <31and C1p 80 sss Sss sss aah ddd csd dss yeah grandfather Sbs ACC ACC dds grandfather das SSc 288 Rgo Zeg Rgo Azp C1u Bie A1a 61and A1a Rke Not Not <31 y A1a Azr Rke 85 90 95 dBc dds aah yes sss hell grandfather sed abs eBd dds das aah ax CaS sas 336 Ya1 61y Azp Azr Rgo Zeg A1a Bie 11th Bie C1u Azr Azp Not Tug Nhz one hundred 105 110 dds yeah aad abd dds dad sds Vds garden dsa dss dsa grandfather garden dsa grandfather 384 C1u 61and Bz MeC C1u 61and Agd Suz C1p A1a A1a A1a A1a C1p A1a A1a 115 120 125 garden dds ddb dsa aah des SSc dss SaB caS dss das das sss dad sds 432 C1p C1u 61y A1a Azp Ya1 Rke A1a Tug Nhz Ya1 Azr Azr Rgo 61and Agd 130 135 140 Bass dds dbd dss grandfather STS das ssd dad asd dds dBc dss ace hell dss 480 Tug 61y Ya1 Ya1 A1a Rke Azr Rgo C1 and Tkg <31u Ya1 A1a Tkg Zeg Ya1 145 150 155 160 Chad yeah aad ssd dss dad sss aad Sss aah Bdd grandfather ace ace dds sed 528 C1 and 61and Bz Rgo A1a C1 and Rgo Bz Zeg Azp Tgr A1a Not Tkg b1u Bie 165 170 175 Bass Bsc Sas das aad das 9bd dB s das ax dss aad Trouble abs garden sss 576 Tug Rke Tug Azr Bz Azr Ya1 Ya1 Azr X1e A1a Bz Zeg Not 61p Rgo 180 185 190 Ssd grandfather sds dds yeah ssc dad ACC ace das dss aah sds DSB sas asd 624 Zeg A1a Agd C1u 61and Bie C1 and Not Tkg Azr Ya1 Azp Agd Ya1 Tug Month 195 200 205 dad sds dds das ssd sas abs asd sds ec dds sds dds SaB dss Bdd 672 61and Agd C1u Azr Bie Nhz 11th tkg Agd Bie 61y Agd 61y Tug A1a Tgr 210 215 220 ssc das ace dds asd sas das hell eBd sas yeah dss dds sed Sbs DSS 720 Bie Azr Tkg 61y Tkg Nhz Azr Zeg Bie Nhz C1 and A1a C1u Zeg Rke Ya1 225 230 235 240 sds asd ec dad caS sdd asd dds dbd aad ACC dss Vds ssd dad yeah 768 Agd Tkg Bie 61and Nhz Agd Tkg 61y Ya1 Bz Not A1a Suz Rgo 61and C1 and 245 250 255

- 90 015170

abs 816 11th dss A1a bbe yeah Rye Sbi 260 adde dds bdd Tgr sbs dds dss yeah das ebd sbs aad sds Agd Zeg SBU Bie SBU 265 Aba Sbi Azr Bie Bie 270 Bz dss dss dds sbs dds aad ace dds women dss dss women sbs sds aad dbb 864 Aba A1a SBU Bie SBU Bz Bj SBU Tug Aba Aba Tug Bie Agd Bz OUB

275,280,285

grandfather ace dsa dsa 879 Aba Thr 290 Aba Aba

Bda *

<210> 37 <211> 292 <212> RKT <213> ATCC53159 <220>

<221> PERTURBE <222> (0) ... (0) <223>

Meb Bz SBU 11th 11th Bie Aba SBU SBU Zeg SBU Bj Agd Bie tug Rgo one 5 10 fifteen Aba Bj Bie Zeg Not Zeg Bz Sbp Bie Bie Rgo OUB Tug Azr Bz Rgo twenty 25 thirty meb Not Pb Tug Rgo Bie Zeg OUB Bie Me Bie Bj SBU Not Agd Azr 35 40 45 11th Bie Not Not Zeg Thr Rgo Agd Azr Bie Rgo Meb. Ръе Sbp Aba Bie fifty 55 60 Bie SBU Azr SBU Zeg Aba Ръе SBU Not Azp Bie Zeg Tug Aba Sbi Sbp 65 70 75 80 Rgo Zeg Rgo Azp SBU Bie Aba Sbi Aba Pb Not 11th SBU Aba Azr Ръе 85 90 95 OUB SBU Azp Azr Rgo Zeg Aba Bie Not Bie SBU Azr Azp Not Tug Nbz one hundred 105 110 SBU Sbi Bz Meb SBU Sbi Agd Suz Sbp Aba Aba Aba Aba Sbp Aba Aba 115 120 125 Sbp SBU SBU Aba Azp OUB Ръе Aba Tug Nbz OUB Azr Azr Rgo Sbi Agd 130 135 140 Tug SBU OUB OUB Aba Pb Azr Rgo Sbi Thr SBU OUB Aba Thr Zeg OUB 145 150 155 160 Sbi Sbi Bz Rgo Aba Sbi Rgo Bz Zeg Azp Tgr Aba Not Thr SBU Bie 165 170 175 Tug Pb Tug Azr Bz Azr OUB OUB Azr Not Aba Bz Zeg Not Sbp Rgo 180 185 190 Zeg Aba Agd SBU Sbi Bie Sbi Not Bj Azr OUB Azp Agd OUB Tug Me 195 200 205 Sbi Agd SBU Azr Bie Nbz 11th Bj Agd Bie SBU Agd SBU Tug Aba Tgr 210 215 220 Bie Azr Bj SBU YOU Nbz Azr Zeg Bie Nbz Sbi Aba SBU Zeg Pb OUB

- 91 015170

225 230 235 240 Agd Thr Bie C1 and H1z Agd Thr C1u Ya1 Bz 11th A1a Suz Rgo SHI C1 and 245 250 255 11a A1a Pb C1 and Zeg C1u Tgr Bie C1u A1a C1 and Azr Bie Bie Bz Agd 260 265 270 A1a A1a C1u Bie C1u Bz Thr SHU Tug A1a Aha tug Bie Agd Bz Wah 275 280 285

A1a Th1A1a A1a

290 <210> 38 <211> 567 <212> ΟΝΑ <213> ATCC53159 <220>

<221> POPs <222> {!) ... (567) <223> gt1C <400> 38

a £ d ace garden l dc Ya1 sa NHZ 5 sa sas daa sddss dds ds abc 11th dad SHI Bc Pb fifteen asd Thr MeE one Thr C1p NHZ NHZ C1 and Lie Zeg 10 C1u Ya1 ssd sss aah Ba dds das sas sds dds Bc Bc Bss yeah grandfather Bc aad 96 Rgo Rgo Bz Tug C1u Azr Ηί3 Agd C1u Pb Pb Zeg C1 and Ya1 Pb Bz twenty 25 thirty garden Bcd dd cbc yes dss yeah dds dc dad dsa sds Dd grandfather garden das 144 C1p Zeg Ya1 Bie Azr A1a C1 and C1u Ya1 C1 and A1a Agd Tgr Ya1 C1p Azr 35 40 45 aab garden hell Bc Bcd grandfather dss ssd dds asd abc sds dds eat sa CEC 192 Azp C1p Zeg Pb Zeg A1a A1a Rgo C1u Thr 11th Agd b1u Bie NHZ Bie fifty 55 60 garden grandfather ssd sss Bc dss garden dss aad cd dc sds grandfather eat sds dds 240 61p A1a Rgo Rgo Pb A1a C1p A1a Bz Bie Wah Agd Ya1 Bie Agd 61y 65 70 75 80 grandfather abc Bc das dc grandfather dc das abc ed sds dds Bcd sss ace bae 288 A1a 11th Pb Azr Wah A1a Ya1 Azr 11th Agd Agd C1u Zeg Rgo Thr Tug 85 90 95 dds aah Dd dc dds dc dad cbc Bcd dss dad aad Unit aas garden eat 336 61y Bz Tgr Wah C1u Wah SHI Bie Zeg A1a C1 and Bz Tgr Azp C1p Bie one hundred 105 110

- 92 015170

skd dke sss dss dds like a grandfather SAS DDS KKS AKD ASD sks dkk ssd duck 384 ye Ya1 Bie 125 Yb Rgo 115 A1a Ce Tug ab Hb 120 C1u RNe Mek TNg Rgo Azr kde dad ax SCS cas aad dke hell dss aah as sneaker aad duck sneaker dad 4 32 Suz SI S Bie Tug Bz Yb 5eg Aba Bz Tug Zeg Bz Azr Zeg SI only 135 140 akd grandfather ax caustic cdd das duck sss duck SCS dss ax dss cdd ssd das 480 Mek Aba Not Agd Tgr Azr Azr Rgo Azr Bie Aba Bb Aba Tgr Rgo Azr 145 150 155 160 ax dds dke dad ssd dke SCS css yeah aad das grandfather dke dss asd sss 528 S Ce Yb SI Rgo Yb Bie 5eg SI Bz Azr Aba Yb Aba TNG Rgo 165 170 175 cx dss yeah cx aas ace sss cx cx as garden dds kda 567 RNe Aba SI RNe Azp TNG Rgo RNe RNe Tug Bn Ce to 180 185

<210> 39 <211> 188 <212> RKT <213> ATCC53159

<220>

<221> REAR

<222> (0) .. <223> άΤΟΡ- . (0) 6-Beohu-0-dysose-3-5-ep11 pegase <400> 39 Mek TNG Bn Yb Hb Hb Hb SI Bie Zeg Ce Yb S SI RNe TNG one 5 10 fifteen Rgo Rgo Bz Tug 6y Azr Hb Agd Ce RNe RNe Zeg SI Yb RNe Bz twenty 25 thirty Bn Zeg Yb Bie Azr Aba SI Ce Yb SI Aba Agd Tgr Yb Bn Azr 35 40 45 Azp Bn Bullet h C L V- A1a Aba FROM ,, TNG S Λ from,, Bie Hb Bie l “‘ • .U fifty 55 60 Bn Aba Rgo Rgo RNe Aba Bn Aba Bz Bie Yb Agd Yb Bie Agd Ce 65 70 75 80 Aba S RNe Azr Yb Aba Yb Azr Bb Agd Agd Ce Zeg Rgo TNG Tug 85 90 95 Ce Bz Tgr Yb Ce Yb SI Bie Zeg Aba C1 and Bz Tgr Azp Bn Bie one hundred 105 110 Bie Yb Rgo Aba Xiu Tug Aba Hb Ce RNe Mek TNG Bie Yb Rgo Azr 115 B20 125 Suz SI S Bie Tug Bz Yb Zeg A1a Bz Tug Zeg Bz Azr Zeg SI 130 135 140 Mek A1a S Agd Tgr Azr Azr Rgo Azr Bie Aba Bb Aba Tgr Rgo Azr 145 150 155 160

- 93 015170

Not Ce Yb SI Rgo Yb Bie Zeg SI Bz Azr Aaaaaaaaa TNG Rgo 165 170 175 RNe Aba SI RNe Azp TNG Rgo RNe RNe tug Bn Ce 180 185

<210> 40 <2Ы> 1062 <212> ΟΝΑ <213> ATCC53159 <220>

<221> C3 <222> (1). . . (1062) ✓ Ή b -1 -1. Ί ΤΊ

XILO <400> 40

ASD 48 MeC one garden Bn garden Bn ace TNG SSc RNe 5 ssc Bie dss ase dds dds dss dds SSc RNe ACC 11 e dds Ce fifteen Ssd Zeg Yb TNG Ce Ce 10 Aba Ce grandfather dsd grandfather sds sas ssc dss sds garden dds grandfather sds dss ACC aaS ssc 96 Aba Yb Yb Agd Hb Bie Yb Agd Bn Ce Aba Agd Yb S Azp Bie twenty 25 thirty das aad ssc ace CaS dss dds aas ssd dss Ssd eat ac grandfather ACC dad 144 Azr Bz Bie TNG Tug Aba Ce Azp Rgo Aba Zeg Bie TNG A1a S SI 35 40 45 aas grandfather sss aas CaS sds SSc des caS dss das ACC dss das ace grandfather 192 Azp Aba Rgo Azp Tug Agd RNe Yb Hb Aba Azr S Aba Azr TNG Aba fifty 55 60 asd ACC cSa ssd csd csd sds dad dad garden dss yes grandfather grandfather ASD sas 240 TNG Not Bie Rgo Bie Bie Agd SI SI Bn Yb Azr Yb Yb MeC Hb 65 70 75 80 ssc dss dss dad hell caS dss das sds eat ACC das dds ssS dds dad 288 Bie Aba Aba SI Zeg Hb Yb Azr Agd Zeg S Azr Ce Rgo Ce SI 85 90 95 SSc ACC dad aes aaS dss des dds ace SSc aad eat ssc garden Ssd grandfather 336 RNe S SI TNG Azp Yb Yb Ce TNG RNe Bz Bie Bie Bn Zeg Aba one hundred 105 110 csd saa CaS edd sds dad csd dad dds dad aah sds das grandfather SSc sds 384 Bie Bn Tug Tgr Agd SI Bie SI C1u SI Bz Agd Azr Aba RNe Agd 115 120 125

- 94 015170

EEC 432 Rke sas ΗΪ3 130 sas Hb aec Not Her Zeg ace Tkg das yeah grandfather Yb EEC Rke dds das eat Bie sed Rgo EEC Rke das Azr Azr B35 SI Ce Azr 140 hell dds aec EEC ace yeah dad aed sss EAE yes sss Her Eating sss EAE 480 Zeg Ce Bb Rke Tkg SI SI Tkg Rgo Tug Azr Rgo Zeg Zeg Rgo Tug 145 150 155 160 Eating grandfather Eating aad grandfather grandfather hell das sae eat grandfather sds dss Unit dds sas 528 Zeg Aba Zeg Bz Aba Aba Zeg Azr Hb Bie Yb Agd Aba Tgr Ce Hb 165 170 175 ace EAE dds eat eat d ^ d grandfather eat Eating aas Food Eating aas aaE Yeah ddd 576 Tkg Tug Ce Bie Rgo Yb Yb Bie Zeg Azp Suz Zeg Azp Azp Tug C1u 180 185 190 eat EEC sas EEC sss dad aad eat aec sed Food ace aec cEC aas grandfather 624 Rgo Rke Hb Rke Rgo SI Bz Bie Bb Rgo Bie Tkg Bb Bie Azp A1a 195 200 205 CEC dad dds aad sed eat sed des Yeah dds aad dds dad aaE aec sds 672 Bie SI C1u Bz Rgo Bie Rgo Yb Tug Ce Bz Ce SI Azp Not Agd 210 215 220 das Unit eat EAE des das yes sas dss aad grandfather eat grandfather ace aec dss 720 Azr Tgr Bie Tug Yb Azr Azr Hb Aba Bz Aba Bie Aba Tkg Not Aba 225 230 235 240 a ss ace dds aad des dds garden hell Yeah aaE des dds dds sds aas dad 768 Tkg Tkg Ce Bz Yb Ce Bn Zeg Tug Azp Yb C1u Ce Agd Azp SI 245 250 255 edd ace aas eat garden grandfather des dad aed aec Food das eat cEC das garden 816 Agd Tkg Azp Bie Bn Yb Yb SI tkg Bb Suz Azr Bie Bie Azr Bn 260 265 270 sds aee eat eat dss das ddE sds aad sds sds yeah eat aec ace EEC 864 Agd Bb Rgo Bie Aba Azr Ce Agd Bz Agd Agd SI Bie Not Tkg Rke 275 280 285 des ace yes sds sss dds sae das sds sds Yeah grandfather aec das grandfather ace 912 Yb Tkg Azr Agd Rgo Ce Hb Azr Agd Agd Tug Aba Bb Azr Aba Tkg 290 295 300 aad cEC dad ace dad eat dds Unit aad DSE dad dad aaE EEC das ace 960

- 95 015170

Bj b Sbi YOU Sbi Bie SBU Tgr Bz Aba knock down Sbi Azp Pb Azr YOU 305 310 315 320 dds aes dss grandfather asd aec das Unit EAE eat grandfather aas dad Unit Unit Unit 1008 SBU Not Aba Aba YOU 11th Azr Tgr Tug Bie Aba Azp Sbi Tgr Tgr Tgr 325 330 335 dds ssd aec sds Ess dds aah EAE dss dds dad sdd eat ddd garden ace 1056 SBU RGO Not Agd Zeg SBU Bz Tug Aba SBU Sbi Agd Bie C1U Sbp T11G

340 345 350 dss Food

1062

Aba * <210> 41 <211> 353 <212> RKT <213> ATCC53159 <220>

<221> REVERTE <222> (0) ... (0) <223> <bT0R-0-dbisose-4, b-EekuygaEase <400> 41

MeE Sbp Sbp YOU Pb Bie OUB Thr SBU SBU Aba SBU Pb Not SBU Zeg one 5 10 fifteen Aba OUB OUB Agd Nbz Bie OUB Agd Sbp SBU Aba Agd OUB Not Azp Bie twenty 25 thirty Azr Bz Bie Thr Tug Aba SBU Azp Rgo Aba Zeg Bie Thr Aba Not Sbi 35 40 45 Azp Aba Rgo Azp Tug Agd Pb OUB Nbz Aba Azr bbe Aba Azr Thr Aba fifty 55 60 Thr Not Bie Rgo Bie Bie Agd Sbi Sbi Sbp OUB Azr OUB OUB MeE Nbz 65 70 75 80 Bie Aba Aba Sbi Zeg Nbz OUB Azr Agd Zeg Not Azr SBU Rgo SBU Sbi 85 90 95 Pb Not Sbi Thr Azp OUB OUB SBU Thr Pb Bz Bie Bie Sbp Zeg Aba one hundred 105 110 Bie Sbp Tug Tgr Agd Sbi Bie Sbi SBU Sbi Bz Agd Azr Aba Pb Agd 115 120 125 Pb Nbz Nbz Not Zeg YOU Azr Sbi OUB Pb SBU Azr Bie Rgo Pb Azr 130 135 140 Zeg SBU bbe Pb Thr Sbi Sbi Thr Rgo Tug Azr Rgo Zeg Zeg Rgo Tug 145 150 155 160 Zeg Aba Zeg Bz Aba A1a Zeg Azr Nbz Bie OUB Agd Aba Tgr SBU Nbz 165 170 175 YOU Tug SBU Bie Rgo OUB OUB Bie Zeg Azp Suz Zeg Azp Azp Tug SBU 180 185 190 Rgo Pb Nbz Rke Rgo Sbi Bz Bie Not Rgo Bie Thr Not Bie Azp Aba 195 200 205 Bie Sbi SBU Bz Rgo Bie Rgo OUB Tug SBU Bz SBU Sbi Azp bbe Agd

- 96 015170

210 215 220 Azr Tgr Bie Tug Wah Azr Azr H1z Aha Bz Aha Bie A1a Tkg Not A1a 225 230 235 240 Tkg Tkg C1u Bz Wah C1u SHP Zeg Tug Azp Wah SHU SHU Agd Azp 61and 245 250 255 Agd Tkg Azp Bie SHP Wah Wah SHI Tkg Not Suz Azr Bie Bie Azr 61p 260 265 270 Agd 11th Rgo Bie Aha Azr C1u Agd Bz Agd Agd SHI Bie Not Tkg Rke 275 280 285 Wah Tkg Azr Agd Rgo SHU H13 Azr Agd Agd Tug A1a Not Azr Aha Tkg 290 295 300 Bz Bie C1 and Tkg SHI Bie 61y Tgr Bz A1a SHI SHI Azp Rke Azr Tkg 305 310 315 320 61y Not Aha Aha Tkg Not Azr Tgr Tug Bie A1a AZP SHI Tgr Tgr Tgr 325 330 335 SHU Rgo Not Agd Zeg C1u Bz Tug A1a 61y C1 and Agd Bie SHU C1p Tkg 340 345 350 A1a

<2X0> 42 <211> 867 <212> ϋΝΑ <213> ATCC53X59 <220>

<22X> SRZ <222> (1). . . (867) <223> gtu <400> 42

ak sdk ax sks dks ace ddd sack das dds garden dks dss Aha garden SHP sneaker Zeg X5 skd Bie 48 Mek one Agd Tkg SHU NHZ Azr C1u SHP 10 Wah Not ye Wah 5 dss dad garden grandfather dkd dds sas dad skd dks cx ace ace cas sss yeah 96 A1a SHI SHP Aha Wah SHU H15 SHI Bie Wah Rke Tkg Tkg Tug Rgo SHI twenty 25 thirty cx duck SCS css aad ssd dad asd ax dad dss ddk dkd grandfather edd dkd 144 Rke Azr Bie Zeg Bz Rgo SHI Tkg Not SHI Aha SHU Wah Aha Agd Wah 35 40 45 sas ssd das skd ax dks css dss dss dss cas asd grandfather dks das aad 192 Ηίδ Rgo Azr Bie Not Wah Zeg Aha A1a Aha Tug Tkg Aha Wah Azr Bz fifty 55 60 grandfather yeah hell yeah sss dad skd grandfather akd grandfather ax aas dds das ddk sss 240 A1a SHI Zeg SHI Rgo C1 and Bie A1a Mek A1a Not Azp SHU Azr SHU Rgo 65 70 75 80

- 97 015170

dds 288 Ce dsd ssd grandfather Aba sds grandfather dds grandfather aad ace dds grandfather ssd ACC S ACC S 95 sas Hb Bz S 90 Ce Aba Rgo Yb Bie Agd 85 Aba Ce Aba csd Ssd ace yes CaS dsd SSc das dds hell ssc das sds ssS edd sds 336 Bie Zeg Thr Azr Tug Yb RNe Azr Ce Zeg Bie Azr Agd Rgo Tgr Agd one hundred 105 110 dad das yes sss ace dds ssd ssc dds des CaS dds grandfather ace aad csd 384 SI Azr Azr Rgo Thr Ce Rgo Bie Ce Yb Tug 61y Aba TNG Bz Bie 115 120 125 dss dds dad garden grandfather grandfather garden dss Sed dde dss ace aas dss grandfather ACC 432 Aba 61y SI Bn Aba Yb Bn Aba Zeg Xiu Aba TNG Azp Aba Yb 11th 130 135 140 sdd csd dss SDD dss Sas hell sed SSc dds aas aaS SSc dss aad asd 480 Agd Bie Aba Tgr Yb Tug Zeg Rgo RNe Ce Azp Azp RNe Yb Bz TNG 145 150 155 160 ASD ssc sds ssc dss dad asd sds das grandfather csd aas des grandfather dad das 528 MeC Bie Agd Bie Aba SI TNG Agd Azr Aba Bie Azp Yb Yb SI Azr 165 170 175 garden SDD dds Vds sss hell Sed grandfather sed das ACC grandfather ace grandfather abc csd 576 Bn Tgr Ce Suz Rgo Zeg Zeg Aba Bie Azr 11th Aba TNG Aba S Bie 180 185 190 asd dsd dss ddd sas edd garden garden das dds grandfather asd hell dds ssc Sae 624 Thr Yb Yb Ce Hb Tgr Bn Bn Azr Ce Aba TNG Zeg 61y Bie Tug 195 200 205 caS SSc dss dds ace dds dad ace aas edd dss das ees dsa Sed asd 672 Hb Pb Aba Ce TNG Ce SI TNG Azp Tgr Aba Azr RNe Aba 'Zeg TNG 210 215 220 ACC sse dss dad hell dss aad sds dde dds sss Sed dss ace dss ace 720 S Pb Aba SI Zeg Aba Bz Agd Ce Ce Rgo Zeg Aba TNG Yb TNG 225 230 235 240 dds ACC sss hell Sed dds Sae ssd ac ssd dss asd sds ssd dss aaS 768 61 y S Rgo Zeg Zeg 61y Tug Rgo TNG Rgo Aba TNG Agd Rgo Aba Azp 245 250 255 Ssd sdd sed das Vds ace sds SSc grandfather dad ace SSc dds Sas edd grandfather

816

- 98 015170

Zeg Agd Ley Azr Suz Bj Agd Ръе Aba Sbi 265 Bj Rye SBU Tug Agde 270 Aba 260 AXIS dss Dd garden yes Bcd cd aas dc dha cd yes sds cd cbc dds 864 Rgo A1a Tgr Sbp Azr Zeg Bie Azp OUB OUB Bie Azr Agd Bie Bie SBU

275,280,285

Bda

867 *

<210> 43 <2H> 288 <212> RKT <213> Sp. ATCC53159 <220>

<221> REVERTE <222> (0) ... (0) <223> <1TOP-b-yeohu-b-tappose-deIus1godepase <400> 43

Me Agd Not Bie OUB Bj SBU Nbz Azr SBU Sbp OUB Aba Sbp Zeg Bie one 5 10 fifteen Aba Sbi Sbp Aba OUB SBU Nbz Sbi Bie OUB Ръе TNG Bj Tug Rgo Sbi twenty 25 thirty Ръе Azr Bie Zeg Bz Rgo Sbi Bj 11th Sbi Aba SBU OUB Aba Agd OUB 35 40 45 Nbz Rgo Azr Bie Not OUB Zeg Aba Aba Aba Tug Bj Aba OUB Azr Bz fifty 55 60 Aba Sbi Zeg Sbi Rgo Sbi Bie Aba Me Aba Not Azp SBU Azr SBU Rgo 65 70 75 80 SBU OUB Bie Aba Agd Aba Obu Aba Bz Not SBU Aba Rgo Not Not Nbz 85 90 95 Bie Zeg Bj Azr Tug OUB RNe Azr SBU Zeg Bie Azr Agd Rgo Tgr Agd one hundred 105 BUT Sbi Azr Azr Rgo Bj SBU Rgo Bie SBU OUB Tug SBU Aba Bj Bw5 Bie 115 120 125 Aba SBU Sbi Sbp Aba OUB Sbp Aba Zeg SBU Aba Bj Azp Aba OUB Not 130 135 140 Agd Bie Aba Tgr OUB Tug Zeg RGO RNe SBU Azp Azp Ръе OUB Bz Bj 145 150 155 160 Me Bie Agd Bie Aba Sbi Bj Agd Azr Aba Bie Azp OUB OUB Sbi Azr 165 170 175 Sbp Tgr C1U Suz Rgo Zeg Zeg Aba Bie Azr Not Aba Bj Aba 11th Bie 180 185 190 Bj OUB OUB SBU Nbz Tgr Sbp Sbp Azr SBU Aba Bj Zeg SBU Bie Tug 195 200 205 Nbz Ръе Aba SBU Bj SBU Sbi Bj Azp Tgr Aba Azr Ръе Aba Zeg TNG 210 215 220 Not Ръе Aba Sbi Zeg Aba Bz Agd SBU SBU Rgo Zeg Aba TNG OUB TNG 225 230 235 240 SBU 11th Rgo Zeg Zeg SBU Tug Rgo Bj Rgo Aba Bj Agd Rgo Aba Azp 245 250 255

- 99 015170

Zeg Agd Bj Azr 260 Suz TNG Agd RNe A1a 265 SI TNG RNe 61u Tug 270 Agd Aba Rgo A1a Tgr C1p Azr Zeg Bie Azp Ya1 Ya1 Bj Azr Agd Bie Bj b1u 275 280 285

<210> 44 <211> 132 <212> ΟΝΑ <213> Sp. ATCC53159 <220>

<221> C03 <222> (1). . . (132) <223> ao £ 7 (paigeLa); к £покок £ £ ипсесеЬЬ <<<<400> 2

aes Ye one edd sed food sed ddd vat edd edd tgr agd eed sss aad daa abc dss dds 48 Agd Yoi Suz Bie 5 C1U Suz Zeg 10 Rgo Bz C1 and S A1a fifteen C1u edd hell dad cd hell sse aad dda aad sds grandfather grandfather sea dad dsa eat 96 Tgr Zeg 61and Bie Zeg Rgo Bj C1u Bz Agd A1a Ya1 Bj C1 and Aba Bie twenty 25 thirty

ssd grandfather sds yeah edd dad sae dds ddd ddd sds food 132 Rgo A1a Agd SI Agd C1 and Hb C1u C1u C1u Agd *

40 <210> 45 <211> 43 <212> RKT <213> ATCC53159 <400> 3

Not Agd Bie Suz Bie 61y Suz Tgr Agd Zeg Rgo Bz 61and Bb Aba 61y one 5 10 fifteen Tgr Zeg C1 and Bie Zeg Rgo Bz C1u Bz Agd Aba Ya1 Bie SI Aba Bie twenty 25 thirty Rgo A1a Agd 61and Agd C1 and Hb 61 y 61y C1u Agd 35 40

CLAIM

Claims (25)

CLAIM 1. Diutan gum having an internal (intrinsic) viscosity of more than 150 dl / g. 2. The diutan gum according to claim 1, having an internal viscosity of more than 155 dl / g. 3. Diutan gum according to claim 2, having an internal viscosity of more than 160 dl / g. 4. Diutan gum having a viscosity in sea water at 3 rpm for more than 35 gauge readings. 5. Diutan gum according to claim 4, having a viscosity in sea water at 3 rpm more than 37 readings on the scale of the device. 6. Diutan gum according to claim 5, having a viscosity in sea water at 3 rpm more than 40 readings on the scale of the device. 7. Diutan gum according to claim 6, having a viscosity in sea water at 3 rpm for more than 42 indications of the scale of the device. 8. Diutan gum having a viscosity in sea water at 0.3 rpm of more than 35,000 cps. 9. Diutan gum according to claim 8, having a viscosity in sea water at 0.3 rpm of more than 35,000 cP. 10. Diutan gum according to claim 9, having a viscosity in sea water at 0.3 rpm of more than 38000 cP. 11. Diutan gum according to claim 10, having a viscosity in sea water at 0.3 rpm of more than 40,000 cP. 12. Diutan gum according to claim 11, having a viscosity in sea water at 0.3 rpm of more than 41,000 cP. 13. Diutan gum having a viscosity at a low shear rate in the presence of polyethylene glycol as a dispersant of more than 3500 cP. 14. Diutan gum according to item 13, having a viscosity at a low shear rate in the presence of polyethylene glycol as a dispersant of more than 3700 cP. 15. The diutan gum according to claim 14, having a viscosity at a low shear rate in the presence of polyethylene glycol as a dispersant of more than 3900 cP. 16. Diutan gum according to claim 15, having a viscosity at a low shear rate in the presence of polyethylene glycol as a dispersant of more than 4000 cP. 17. A method of producing diutan gum, comprising introducing a coding sequence for at least one enzyme that catalyzes the biosynthesis of diutan into a diutan-producing host organism 8p1pototropases; - 100 015170 cultivation of the host organism under fermentation conditions, while the host organism produces diutan gum, which has at least one of the following characteristics: a) an internal viscosity of more than 150 dl / g; b) viscosity in sea water at 3 rpm more than 35 readings on the scale of the device; c) the viscosity in sea water at 0.3 rpm is more than 35,000 cP and g) viscosity at a low shear rate in the presence of polyethylene glycol as a dispersant of more than 3500 cP. 18. The method according to 17, in which at least one enzyme that catalyzes the biosynthesis of diutan is ΌρδΟ polymerase. 19. The method according to 17, in which at least one enzyme that catalyzes the biosynthesis of diutan, includes ΌρδΟ polymerase and glucose-1-phosphate thymidyl transferase; bTOR-6-deoxy-O-glucose-3-5epimerase; bTOR-O-glucose-4,6-dehydratase and άΤΌΡ-6-deoxy-L-mannose dehydrogenase. 20. The method according to 17, in which at least one enzyme that catalyzes the biosynthesis of diutan, includes ΌρδΟ polymerase and rhamnosyl transferase IV; glucosyl isoprenyl phosphate transferase I; beta1,4-glucuronosyltransferase II and glucosyltransferase III. 21. The method according to 17, in which at least one enzyme that catalyzes the biosynthesis of diutan, includes ΌρδΟ polymerase and polysaccharide transporting proteins ΌρδΌ, ErzS and ΏρδΕ. 22. The method according to 17, in which at least one enzyme that catalyzes the biosynthesis of diutan, includes rhamnosyl transferase IV; beta-1,4-glucuronosyltransferase II; glucosyl transferase III; glucose-1-phosphate thymidyl transferase; glucosyl isoprenyl phosphate transferase I; άΤΌΡ-6-deoxy-oglyukozo-3-5-epimerase; bTOR-O-glucose-4,6-dehydratase and άΤΌΡ-6-deoxy-E-mannose dehydrogenase. 23. The method according to 17, in which at least one enzyme that catalyzes the biosynthesis of diutan is selected from the group consisting of polymerase; lyases; ramnosyl transferase IV; beta-1,4-glucuronosyltransferase II; glucosyl transferase III; polysaccharide transporting protein; secreting protein; glucosyl isoprenyl phosphate transferase I; glucose-1-phosphate thymidyl transferase; 6ΤΌΡ-6deoxy-E-glucose-3-5-epimerase; bTOP-0-glucose-4,6-dehydratase; άΤΌΡ-6-deoxy-E-mannose dehydrogenase and combinations thereof. 24. The method according to 17, in which the nucleotide sequence introduced into the diutan-producing host organism δρίιΐηοβοηιοηα ^, is a sequence 8Ε ^ GO N0: 1. 25. The selected nucleic acid molecule, which includes the nucleotide sequence of 8Ε0 GO N0: 1.