KR101372110B1 - High viscosity diutan gums and methods of producing - Google Patents

Abstract

The present invention describes the preparation of diutane polysaccharides which exhibit increased viscosity properties compared to previous prepared polysaccharides having the same type of repeat units. These improved diutan polysaccharides include Sphingomonas , which contain a broad host-wide multicopy plasmid, in which genes for biosynthesis of diutan polysaccharides have been cloned. sp . ) Through the production of derivatives of ATCC 53159. The plasmid provides the ability to produce multiple copies of genes for such polysaccharide synthesis in a host sphingomonas strain. In this way, a method of increasing the production of the target diutan polysaccharide is provided, as well as a method of producing the diutan polysaccharide whose physical properties (high viscosity characteristics described above) are improved. These diutan polysaccharides have proven to be particularly useful as thickeners that can be used in cement materials and in the field of oilfield. Also included in the present invention are methods according to the invention for producing such improved diutan polysaccharides, and novel cloned genes necessary for producing improved diutane using this method. In addition, novel genetically engineered Sphingmonas strains comprising the required DNA sequence are included in the present invention.

Description

HIGH VISCOSITY DIUTAN GUMS AND METHODS OF PRODUCING

The present invention describes the preparation of diutane polysaccharides which exhibit increased viscosity properties compared to previous prepared polysaccharides having the same type of repeat units. This improved diutan polysaccharide is a Sphingomonas species that contains a broad host-wide multicopy plasmid in which the gene for biosynthesis of diutan polysaccharide is cloned. sp . ) Through the production of derivatives of ATCC 53159. The plasmid provides the ability to produce multiple copies of genes for such polysaccharide synthesis in a host sphingomonas strain. In this way, a method of increasing the production of the target diutan polysaccharide is provided, as well as a method of producing the diutan polysaccharide whose physical properties (high viscosity characteristics described above) are improved. These diutan polysaccharides have proven to be particularly useful as thickeners that can be used in cement materials and in the field of oilfield. Also included in the present invention are methods according to the present invention for producing such improved diutan polysaccharides, and novel cloned genes necessary for producing improved diutan using this method. In addition, new genetically engineered sphingomonas strains comprising the required DNA sequences are also included in the present invention.

Polysaccharides or gums are mainly used to thicken or gel aqueous solutions and are often classified into two groups, thickeners and gelling agents. Typical thickeners include starch, xanthan gum, diutan gum, wellan gum, guar gum, carboxymethylcellulose, alginate, methylcellulose, gum karaya and gum tragacanth. Common gelling agents are gelatin, gellan gum, starch, alginate, pectin, carrageenan, agar and methylcellulose.

Some polysaccharides, or more specifically, biogum, such as xanthan, gellan, wellan and diutan, have been produced through fermentation from microorganisms for many years. Such biogum exhibits a number of properties, such as the ability to change viscosity, which has been used in many different applications. Included in this list are gelling agents for foods such as confectionery jelly, jams and jellies, dessert gels, sugaring and dairy products, as well as components of microbial media. Thickeners are also utilized in numerous end use applications to change the viscosity of the target liquid. There may be many other different possible end uses (including cement production, for example), but the ability of the gums to make viscosity changes in underground and / or underwater petroleum liquids to facilitate their flocculation is of particular interest. . Different biogum is made from different bacterial sources, for example Xanthan gum Xanthomonas campestris ), gellan gum from Sphingomonas elodea , wellan gum from sphingomonas species ATCC 31555, and diutan gum (S-657) from sphingomonas species ATCC 53159. Genetic engineering of these strains has been undertaken in the past to make significant changes to the synthetic gum materials prepared through the fermentation process described above. This manipulation causes a change, such as the removal of acyl groups, resulting in different gum materials exhibiting different physical properties. In general, these genetic manipulations include types that ultimately alter the composition of the target biogum through altered gene expression in the host organism, or types that increase the yield of the target biogum through the introduction of plasmids representing only gene amplification (Pollock). US Pat. Nos. 5,854,034, 5,985,623 and 6,284,516, and US Pat. No. 6,709,845 to Pollock.

Diutan gum (also known as heteropolysaccharide S-657) is prepared through fermentation of strain sphingomonas species ATCC 53159 and shows thickening, suspending and stabilizing properties in aqueous solution. Diutan generally exhibits hexameric repeating units consisting of the four sugars of the main chain (glucose-glucuronic acid-glucose-rhamnose), and the side chains of two rhamnose residues attached to one of the glucose moieties. A detailed description of the diutan gum structure can be found in Chowdhury, TA, B. Lindberg, U. Lindquist and J. Baird, carbohydrate Research 164 (1987) 117-122. Di wootan are each repeating unit the literature as having two acetyl substituent have been described in [Diltz et al., Carbohydrate Research 331 (2001) 265-270]. Both of these references are incorporated herein by reference in their entirety. A detailed description of the preparation of diutan gum can be found in US Pat. No. 5,175,278, which is incorporated herein by reference in its entirety. Diutane can be prepared from sphingomonas strains, for example, using standard fermentation techniques using carbohydrate sources (including but not limited to glucose, maltose and the like), nitrogen sources and additional salts.

Physical properties imparted by wild type diutan biogum are particularly desirable for certain industries in terms of viscosity change properties and / or moisturizing properties. Unfortunately, Diutan has proven difficult to manufacture cost-effectively. In addition, this cost problem currently has a disadvantageous effect on the widespread use of diutan, where the viscosity seen by biogum is insufficient to replace other inexpensive but effective biogum (eg, xanthan gum). Because. Thus, there has been a clear need, at least, to provide a method for producing an effective diutan at low cost, and / or a method for producing a biogum of the diutan type which is also significantly improved in physical properties. To date, methods of making any type of related sphingan have been mentioned only in terms of yield improvement (specifically, without any demonstration of diutan) (mentioned in the Pollock et al. Patents described above). There was no discussion or appropriate proposal for any method of providing a method for producing high molecular weight, improved diutan gum, which showed any improvement in viscosity measurement by the above production method.

Summary of the Invention

The inventors have found that amplifying certain novel isolated DNA sequences for diutan biosynthesis in a host sphingomonas organism not only increases the production of diutan gum derived from this sequence, but also exhibits increased viscosity characteristics. It was found to produce Utan gum. This novel DNA sequence, which is introduced into the host organism via any well known method such as, but not limited to, a plasmid, provides the desired results and is used in the diutane synthesis method. This particular advantage of using the amplified genes in the plasmid is that it is relatively simple to integrate such isolated DNA sequences into the diutane synthesis process. Another advantage is the ability to generate high viscosity characteristics in the target diutan gum, potentially increasing fermentation production efficiency if necessary.

Thus, the present invention provides improvements, in particular, in many different viscosity measurements

i) intrinsic viscosity greater than 150 dL / g, preferably greater than 155 dL / g, more preferably greater than 160 dL / g;

ii) 3 rpm viscosity of seawater above dial 35, preferably above 37, more preferably above 40, most preferably above 42;

iii) 0.3 rpm viscosity of seawater above 35,000 centipoise (cP), preferably above 39,000 cP, more preferably above 40,000 cP, most preferably above 41,000 cP; And PEG low shear viscosity above 3500 cP, preferably above 3700 cP, more preferably above 3900 cP, and most preferably above 4000 cP.

Diutan gum that represents. In addition, the present invention provides the above-described diutan gum by introducing a specific bundle of genes into a host sphingomonas organism, as defined in any of the foregoing sections, and obtaining a diutan gum produced by fermentation in the organism. It includes how to do it. The invention also encompasses certain DNA sequences and any vectors (such as plasmids) for providing multiple copies of a gene or for enhancing expression of a gene using stronger promoters and the like. In addition, genetically modified strains of sphingmonas containing plural copies of the diutan biosynthetic gene defined by the unique isolated DNA sequence are also included.

Such unique isolated DNA sequences have been found to require one or more diutan biosynthetic enzymes, ie DpsG polymerases. In another possible embodiment, the diutan biosynthetic enzyme is selected from DpsG polymerase and glucose-1-phosphate thymidylyltransferase; dTDP-6-deoxy-D-glucose-3-5-epimerase; dTDP-D-glucose-4,6-dehydratase; And dTDP-6-deoxy-L-mannose-dehydrogenase. In another possible embodiment, the diutan biosynthetic enzyme comprises DpsG polymerase and rhamnosyl transferase IV; Beta-1,4-glucuronosyl transferase II; Glucosyl isoprenylpoate transferase I; And glucosyl transferase III. In another possible embodiment, the diutan biosynthetic enzyme comprises dpsG polymerase and polysaccharide export proteins dpsD, dpsC and dpsE. In another possible embodiment, the diutan biosynthetic enzyme is selected from rhamnosyl transferase TV; Beta-1,4-glucuronosyl transferase II; Glucosyl isoprenylpoate transferase I; Glucosyl transferase III; Glucose-1-phosphate thymidylyltransferase; dTDP-6-deoxy-D-glucose-3-5-epimerase; dTDP-D-glucose-4,6-dehydratase; And dTDP-6-deoxy-L-mannose-dehydrogenase. In general, the diutan biosynthetic enzymes of the process according to the invention and the diutan biosynthetic enzymes in the products according to the invention are polymerases; Lyase; Rhamnosyl transferase IV; Beta-1,4-glucuronosyl transferase II; Glucosyl transferase III; Polysaccharide transport protein; Secreted protein; Glucosyl-isoprenylphosphate transferase I; Glucose-1-phosphate thymidylyltransferase; dTDP-6-deoxy-D-glucose-3-5-epimerase; dTDP-D-glucose-4,6-dehydratase; dTDP-6-deoxy-L-mannose-dehydrogenase and combinations thereof. The present invention is one as shown in SEQ ID NOs: 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43. Or more diutan biosynthesis enzymes, or SEQ ID NOs: 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43 and 95 It further comprises an isolated nucleic acid molecule (in addition to DNA that may be present on the target chromosome) encoding at least% identical enzymes.

Thus, the process according to the invention (also the product produced by this process) relates to sphingum gums, in particular diutan types, including but not limited to S88, S60 and S657.

As described above, the present invention has been accomplished by discovering and realizing that a specific DNA sequence introduced into a plurality of copies within a particular sphingomonas strain can increase the biosynthetic production of high viscosity diutan polysaccharides. Genetically engineered bacteria containing genes for increased production produce significantly higher amounts of diutan polysaccharides than nongenic engineered bacteria, resulting in the high viscosity properties of the aforementioned results.

According to the present invention, host organisms (any well-known form, such as also, in order to obtain the above-mentioned increase in production and increase in viscosity properties (not supported by any particular scientific theory, but through which the molecular weight range properties are believed to increase) DNA sequences introduced into plasmids, by way of non-limiting example, can be isolated, recovered and cloned by techniques readily available in the art. The DNA is then transferred to a plurality of copyings (via plasmids, in other known ways) to sphingomonas bacteria, or the expression of the gene is increased via a suitable, e.g., more potent, promoter. After insertion into the target bacterium, the production of diutan can be measured by fermenting the genetically engineered bacterium and comparing the yields in terms of amount produced and quality produced. Both increased production and viscosity increase can be measured by comparing the production of diutan over the wild type diutan producing strain (ATCC 53159) via the method according to the invention.

Brief Description of Drawings

1 is a plot of isolated genes for diutan gum biosynthesis. Presumptive or known genes are indicated. Segments inserted into different plasmids are also indicated.

2 is a graph of the improvement in intrinsic viscosity measurement achieved by the diutan biogum material according to the invention.

details

The following terms will be used throughout this specification in connection with the present invention and have the meanings specified below:

The term "sphingmonas" is used throughout this specification to refer to strains of Gram-negative bacteria from the genus Sphingomonas.

The term "increased producer" or "increased production" is used throughout this specification to produce significantly more (more than about 5% more on a weight / weight basis) diutan polysaccharide compared to wild-type bacteria of the same strain. Used to describe genetically engineered bacteria containing multiple copies of DNA sequences isolated from strains.

The term “isolated” is intended to be recovered from a microorganism and subjected to some degree of purification, ie one or more purification steps, cleaved or cleaved by restriction enzymes, cloned into a plurality of copies, inserted into a plasmid vector or inserted into or incorporated into a bacterium. Used to describe DNA that can be.

The term "sequence" is used to describe a specific segment of DNA identified by a nucleotide unit. The term "inserted" is used throughout the present specification to describe the process and results of delivering (eg, by way of non-limiting example, via a plasmid) DNA fragments isolated from the chromosomal DNA of a diutan producing sphingmonas strain to the sphingmonas strain. Used for. The isolated DNA is first introduced into a preferred plasmid (here pLAFR3), which is also well known in the art as a non-limiting example, and then to be delivered to the receptor sphingomonas bacteria, for example by conjugation or activation. Can be. After insertion into the receptor sphingomonas bacterium, the plasmid containing the relevant DNA sequence can be used to increase the production of several (more than two, typically 4-10) high-viscosity (also thought to be high molecular weight ranges) diutane polysaccharides. Replication will be done at the receptor cell to provide) DNA fragments. It is generally effective to use conjugation or maneuver to deliver plasmid vectors to receptor bacteria. In addition, chemical transformation or electroporation of competent cells using purified DNA may be used. Other vectors or bacteriophages can be used to deliver DNA to the host cell. There is no need to maintain DNA segments in plasmids (or other well-known delivery vectors) in the receptor diutan producing sphingmonas. It is common to introduce additional copies of the DNA segments into the bacterial chromosomes so that the segments are replicated every time by the same mechanism of replicating the bacterial DNA. Alternatively, increased gene expression can be achieved using stronger promoter elements.

The term "gene amplification" refers to an enhanced gene copy, for example, by cloning a multicopy plasmid (such as 4-10) into a target gene or inserting multiple copies of the gene (such as 4-10) into the bacterial genome, or Alternatively, it is used to indicate enhanced gene expression by changing promoter elements to enhance gene expression. Both of these and other methods can increase the amount of encoded protein.

The term "biosynthesis" is used throughout this specification to describe the biological production or synthesis of diutan by sphingmonas bacteria. Diutan polysaccharides are synthesized from individual carbohydrate units in a series of steps controlled by a number of bacterial enzymes.

Relevant DNA sequences incorporated into any selected form (e.g., again, but not necessarily, but not necessarily, in the form of plasmids), may contain genetic information known to be beneficial or essential for enhanced production and biosynthesis of increased molecular weight diutan polysaccharides. Coding In addition, however, certain DNA sequences according to the present invention (such as those within plasmid pS8), while not supporting specific scientific theories, not only increase production but also increase the number of repeat units polymerized in the individual polymers of the diutan itself. I think that. As a result, the increase in repeat units as described above is surprisingly believed to generate the high viscosity properties provided by diutan gum. The molecular weight increase has been assumed to be due to the measured increase in intrinsic viscosity related to the molecular weight, by a power law relationship. Thus, for linear polymers (such as diutan gum), it is known that the intrinsic viscosity is substantially proportional to the molecular weight in that respect.

Isolation of the relevant DNA sequences which are the basis of the method according to the invention and which produce diutane polysaccharides of increased viscosity is accomplished by standard techniques and methods. Thus, such sequences can be produced from Diutan producing Sphingmonas strains cultured using standard procedures. The extraction of DNA can then be carried out, for example, by centrifugation and resuspension of the initial bacterial cell, followed by elution of the DNA through subsequent purification columns. After purification is complete, the isolated DNA can be digested with restriction endonucleases, cloned into the desired plasmid or other transfer vector, and then transferred to the receptor strain. Other techniques as known in the art can be used without limitation.

DNA cloning in the present invention is in accordance with general techniques and methods that have become standard in the art. It is noted that a number of methods can be used to clone DNA fragments according to the invention, and the invention is not limited to, for example, using plasmid cloning vectors. For example, the DNA fragment can be inserted into a bacteriophage vector and cloned.

The cloned DNA sequence can then be introduced into the sphingmonas strain via a plasmid or other transfer vector. The genetically modified sphingomonas strain can then be used to produce diutan by fermentation. Basically, a medium suitable for fermentation is generally a carbohydrate, nitrogen source, for example inorganic ammonium, inorganic nitrates, organic amino acids or proteinaceous materials, including carbon sources such as glucose, lactose, sucrose, maltose or maltodextrin, Such as hydrolyzed yeast, soy flour or casein, distiller's soluble or corn immersion water and inorganic salts. A wide variety of fermentation media will be supported for the preparation of the diutan according to the invention.

Carbohydrates may be included in the fermentation broth in varying amounts, but are typically included in about 1-10% by weight (preferably 2-8% by weight) of the fermentation broth. Carbohydrates may be added prior to fermentation or alternatively during fermentation. The amount of nitrogen may range from about 0.01% to about 0.4% by weight of the aqueous medium. A single carbon source or nitrogen source can also be used as a mixture of these sources. Inorganic salts used to ferment sphingomonas bacteria are in particular salts containing sodium, potassium, ammonium, nitrate, calcium, phosphate, sulfate, chloride, carbonate and similar ions. Trace metals such as magnesium, manganese, cobalt, iron, zinc, copper, molybdenum, iodide and borate salts may also be advantageously included.

Fermentation may be carried out at a temperature in the range of about 25 ° C. to 40 ° C., preferably in the range of about 27 ° C. to 35 ° C. Inoculum can be prepared by standard methods of volume scale-up, including shaking flask culture and small scale in-situ fermentation. The medium for preparing the inoculum may be the same as the production medium or may be any of several standard media well known in the art, such as Luria broth or YM medium. More than one seed stage can be used to obtain the desired volume for inoculation. Typical inoculation volumes range from about 0.5% to about 10% of the total final fermentation volume.

The fermentation vessel may comprise a stirrer for stirring the contents. The vessel may also be equipped with automatic pH and foaming regulators. The production medium can be added to the container and sterilized by heating in place. Alternatively, carbohydrate or carbon sources can be sterilized separately before addition. Pre-incubated inoculation culture can be added to the cooled medium (typically at a preferred fermentation temperature of about 27 ° C. to about 35 ° C.), and the stirred culture is fermented for about 48 hours to about 110 hours, High viscosity broths can be created. Diutane polysaccharides can be recovered from the broth as a standard method of precipitation using alcohols, generally isopropanol.

Including a detailed description of the drawings

Preferred of the present invention Concrete example

The following examples are provided to illustrate the invention. The description of the embodiments should not be construed as limiting the scope of the invention in any manner.

DNA  Sequence Separation / Plasmid Preparation

In order to perform initial isolation and determine sequences suitable for the results of the invention described above, a genetic library of ATCC 53159 organisms was prepared as follows: Chromosome DNA was isolated from sphingomyosis ATCC 53159 and Sau 3AI restriction endo Partially digested with nuclease. DNA fragments ranging from 15-50 kb were purified from agarose gels and bound to the cosmid cloning vector pLAFR3 digested with Bam HI (Staskawicz, et al, "Molecular characterization of cloned avirulence genes from race 0 and race 1). of Pseudomonas syrinae pv. Glycinea ", J. Bacteriology 1987. 169: . Conforms to 5789-94), Escherichia coli (Escherichia coli ) was isolated from strain JZ279 (Harding, et al., "Genetic and physical analysis of a cluster of genes essential for xanthan gum biosynthesis in Xanthomonas campestris ", J.Bacteriology 1987. 169:. . 2854-61) combined reactants λ phage particles (from packaged using a Gigapack III Gold packaging extract from California, La Jolla material Stratagene), E. coli and Library Efficiency (E . coli) DH5αMCR cells (were transfected by Life Technologies) in the United States Rockville, Md material. Approximate were pooled 10,000 tetracycline-resistant colonies to form a gene library, was isolated the individual sequences from the library, in which case Work carried out included isolating specific genes for polysaccharide biosynthesis from the sphingmonas ATCC 53159 organism.

Such genes for polysaccharide biosynthesis are generally identified by the complementarity of mutations that are defective in polysaccharide synthesis, especially those blocked in the first step (glycosyl transferase I). Since the transferase I lacking mutations of ATCC 53159 are not available initially, the complementarity of the transferase I lacking mutations of Sphingmonas elodea and xanthomonas campestris is used to identify genes for diutan polysaccharide biosynthesis. It was. Plasmid pLAFR3 can be transferred from its E. coli host to other Gram-negative bacteria by tri-parental conjugation using a helper plasmid that provides IncP delivery function [Ditta, et al., "Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti ", according to Proc. Natl. Acad. Sci. 1980. 77 : 7347-51.]. The RK2 plasmid has an estimated copy number of 5 to 7 per chromosome in this coli (Figurski et al.," Suppression of ColE1 replication properties by the Inc P-1 plasmid RK2 in hybrid plasmids constructed in vitro ", J. MoI. Biol. 1979 133 : 295-318.).

The gene library of ATCC 53159 chromosomal DNA in this coli is transferred to the nonmucoid mutation (GPS2) of S. eldeaea ATCC 31461 by triad conjugation, which is selected for tetracycline and streptomycin resistance. The helper plasmid used was pRK2013 (in this E. coli strain JZ279) which contained a narrow host range of origin of replication but exhibited the trans-functional function required to activate pLAFR3. Plasmid pRK2013 did not replicate in Sphingmonas strains. S. Elodea ATCC 31461 produces polysaccharide gellans. Both the gellan and the diutan polysaccharide are [→ 4) -α-L-rhamnose- (1 → 3) -β-D-glucose- (1 → 4) -β-D-glucuronic acid- (1 → 4 have the same tetrasaccharide repeating unit consisting of) -β-D-glucose- (1 →], however, the diutan also comprises a side chain of two rhamnose molecules attached to one of the glucose residues and is denatured by acetyl On the other hand, the gellans do not have side chain sugars and are denatured by acetyl and glyceryl The mutant GPS2 is the first step in polysaccharide biosynthesis, ie glucose-1 phosphate is UDP-D-glucose by the glucosyl transferase I enzyme. Defects in the delivery to the bactoprenyl phosphate lipid carrier at .. In tetracycline selection plates, polysaccharide production (mucoid) colonies were isolated from the background of non-mucolytic colonies. Coding Laze I Contains ATCC 53159 gene and approximately 20-25 kb of contiguous DNA. Plasmid DNA was isolated from eight mucinous GPS2 transconjugants and transferred to this E. coli strain DH5α (Life Technologies) by electroporation. The plasmid was isolated from this coli to obtain sufficient DNA for double digestion using restriction endonuclease Hind III / Eco RI (cutting both sides of the Bam HI restriction endonuclease site in the polylinker) to insert the insert DNA. The size of the insert DNA in the clone was determined by gel electrophoresis The terminal sequence of some plasmids was determined by sequencing from primers specific for the plasmid sequence flanking the Bam HI site of the vector. Sequences were analyzed by comparison with sequences in a computer database using BLASTX, two plasmids pS8 and pS6 are shown in FIG. And Similarly, ATCC 53159 gene libraries, Article 3 through the junction, transferase I (CXC109) (which will be the same as in the literature, such as Harding described above) is devoid rifampicin resistance X boiling liquid. It was delivered to Campestris mutants and selected for resistance to tetracycline and rifampicin. X. Campestris produces xanthan polysaccharides, the synthesis of which is also initiated by the transfer of glucose-1-phosphate from UDP-D-glucose to bactoprenyl phosphate lipid carriers by transferase I enzymes ( Ielpi et al., "Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthodmonas compestris ", J. Bacteriology. 1993. 175 : 2490-500). The plasmid was purified from the mucin-transfected strain and the terminal sequence was determined as described above. Two plasmids pX6 and pX4 are shown in FIG. .

S657 DNA cloned from plasmids pS8 and pX6 were fully sequenced by double stranded shotgun sequencing at Lark Technologies Inc. (Houston, TX). These sequences were analyzed to identify genes for diutan biosynthesis (shown in FIG. 1). Gene function is characterized by other genes in the database, particularly published genes for biosynthesis between S-88 sphinxes (e.g., in the patents of '516 Pollock et al., Supra), GenBank Accession No. U51197 and Gellan (GenBank AY217008 and AY220099). Same sex was specified as a reference. The gene was found to encode transferases for four sugars of the backbone and four genes for dTDP-rhamnose synthesis (FIG. 1). Genes for the secretion of polysaccharides were based on homology to genes for biosynthesis of other polysaccharides. Both genes encode proteins that are homologous to proteins involved in protein secretion. Both genes presumably encode polymerase and lyase. The insert in plasmid pX6 contains 17 genes, including the gene dpsB encoding transferase I (which initiates the first step in diutan synthesis), the gene for secretion and four genes for dTDP- rhamnose synthesis. One lacks genes for transferases II, III and IV and putative genes for polymerases and lyases. Plasmid pS8 is a group of dps gene bundles containing genes for the secretion of polysaccharides, including all four backbone sugar transferases, four genes for dTDP-rhamnose synthesis, and putative genes for polymerase and lyase. Contains dog genes, but lacks genes of unknown function, orf6 and orf7 . Plasmid pS6 contains a gene for secretion and four sugar transferases, but no genes for polymerase or all of the genes for dTDP-rhamnose synthesis. Plasmid pX4 contains only a small portion of the dps region, but by genes encoding transferase I and Pollock et al., Described as sufficient to increase the production of polysaccharides in sphingomonas strains, for dTDP-rhamnose synthesis It contains four genes.

Strain manufacturing

The four plasmids described above were then subjected to sphingomonas strain ATCC No. 1 through triparental conjugation as described above. It was introduced into 53159 to form novel S657 genetically engineered strains (S657 / pS8, S657 / pS6, S657 / pX6 and S657 / pX4). Thereafter, the fermentation was carried out as described above to produce a biogum material as described below. While all four plasmids had a beneficial effect on diutan productivity, the pS8 plasmid also surprisingly provided a very large increase in diutane viscosity and an increase in molecular weight. The DNA sequence of pS8 (26278 bps) (DNA SEQ ID NO: 1) is provided, and the encoded genes are listed in Table 1 below, which is shown in diagram form in FIG. 1. Plasmid DNA inserts in the pS8 comprises a portion of a gene from dpsG to rmlD, and gene dpsS and orf7.

The gene table below is basically a list of genes represented by DNA sequences for insertion into plasmid pS8, as shown in FIG.

pS8  Gene on plasmid insert start End designation Explanation 2 ^*  1054  dpsS homologous with gelS (partially)   2738  1113 C  dpsG  Putative polymerase   4895  2898 C  dpsR  Putative lyase   5093  6031  dpsQ  Putative Rhamnosyl Transferase IV   7082  6111 C  dpsI  Unknown   7121  8167  dpsK  Beta-1,4-glucuronosyl transferase II   8164  9030  dpsL  Glucosyl Transferase III  10467  9079 C  dpsJ  Unknown  11076  12374  dpsF  Unknown  12389  13306  dpsD  Putative polysaccharide transport protein  13341  14687  dpsC  Putative polysaccharide transport protein  14687  15394  dpsE  Putative polysaccharide transport protein  15405  16286  dpsM  Putative polysaccharide transport protein  16270  16968  dpsN  Putative polysaccharide transport protein  18454  17060 C  atrD  Putative secreted protein  20637  18451 C  atrB  Putative secreted protein  21229  22641  dpsB  Glucosyl-Isprenylphosphate Transferase I  22757  23635  rmlA  Glucose-1-phosphate thymidylyl transferase  23632  24198  rmlC  dTDP-6-deoxy-D-glucose-3-5-epimerase  24202  25263  rmlB  dTDP-D-glucose-4,6-dehydratase  25263  26129  rmlD  dTDP-6-deoxy-L-mannose-dehydrogenase 26277 ^*  26146 C  orf7  (Partial) unknown function ^{* No} first in-frame codon, start codon

Diutan  Produce

Diutane production using the genetically engineered plasmid containing Sphingomonas S657 strain, compared to the S657 wild type strain without plasmid, was measured by carrying out three sets of fermentations with stirring and aeration in the same liquid medium in an Applikon 2 O L fermentor. . To the plasmid containing strain, antibiotic tetracycline was added at 5 mg / L during fermentation to ensure preservation of the plasmid. KOH was added as needed for pH adjustment. Two inoculation steps were performed using 1% to 6% inoculum inoculation. The medium used for fermentation contained corn syrup as a carbohydrate source, an assimilable nitrogen source and salts. Nutrients that can be used for fermentation are well known in the art and include carbohydrates such as glucose, sucrose, maltose or maltodextrin, nitrogen sources such as inorganic nitrogen such as ammonium or nitrate, organic nitrogen such as amino acids, Hydrolyzed yeast extract, soy protein, or corn steep water, and additional salts containing, for example, chloride, phosphate, sulfate, calcium, copper, iron, magnesium, potassium, sodium or zinc.

As a measure of the resulting diutane yield, broth viscosity and precipitated fibers were measured. The viscosity of the fermentation broth was measured through a Brookfield viscometer operated at 60 rpm with spindle # 4, the results of which are shown in Table 2. At the end of the fermentation, the broth was treated with the well known introduction of glucoamylase enzymes to hydrolyze any residual oligosaccharides derived from corn syrup. The produced diutan gum was then precipitated from one aliquot of broth using two volumes of isopropyl alcohol. The fibers were collected in a filter and dried. In Table 2, the term DWY refers to the total dry weight yield of biogum that can be precipitated after hydrolysis of excess oligosaccharides derived from corn syrup.

Obviously, the material produced is higher in yield with plasmids pX4, pX6, pS6 or pS8, which carry additional copies of the genes for the diutan biosynthesis present therein. However, when using the pS8 plasmid, the broth viscosity increased significantly compared to the increase in dry weight yield, indicating that some factors influenced the viscosity in addition to the increase in the amount of diutane produced.

Fermentation of Plasmid-Containing Strains
DWY
Strain
Conduct # 1
Conduct # 2
Conduct # 3
Average %
increase  S657 34.3 32.2 33.9 33.5 ----  S657 / pS8 37.1 35.4 35.9 36.1 8.0%  S657 / pX6 38.4 37.6 33.5 36.5 9.1%  S657 / pS6 37.6 12.3%  S657 / pX4 36.4 8.8%
Bros  Viscosity
Strain
Conduct #l
Conduct # 2
Conduct # 3
Average %
increase  S657 5150 4950 5550 5217 ----  S657 / pS8 6650 6850 6850 6783 30.0%  S657 / pX6 5400 6250 5125 5592 7.2%  S657 / pS6 6675 28.0%  S657 / pX4 5525 5.9%

Obviously, the material produced was higher in yield due to any of the four plasmids present therein, while the pS8 and pS6 plasmids surprisingly significantly increased the broth viscosity, indicating a high production quality. The quality of the resulting diutan gum product, ie the viscosity, was then measured.

In the application test Diutan Rheology

The diutan gum samples were then analyzed in terms of potentially beneficial uses within two different zones: dielectric additive for oil recovery and cement additive for moisturizing and rapid installation.

The oilfield industry is based on what is referred to as a "sea water viscosity (SWV)" test as an acceptable method of performance for oil recovery gums. This test is basically a measure of the effectiveness of the gum to increase the viscosity at brine conditions of water (eg to repeat recovery from the seabed).

Predicting the availability of the gum produced as a suitable viscosity modifier for oil recovery purposes is generally accepted in view of the change in viscosity of the test seawater formulation. This "Synthetic Seawater" formulation is prepared by mixing 419.53 grams of sea salt (ASTM D-1141-52) in 9,800 grams of DI water. For seawater viscosity testing, 0.86 grams of sample gum is added to 307.0 g of Synthetic Seawater and mixed for 35 minutes at approximately 11,500 rpm in a Fann Multimixer (Model 9B5, part number N5020). At the end of 35 minutes, the solution is cooled to approximately 26 ° C. and then the viscosity is measured. For 3-rpm readings, place the sample on a fan sample platform (Fann model 35A; Torsion spring MOC 34/35 F0.2b; Bob B1; Rotor R1), rotate the motor at low speed and turn the gear converter Set it to the middle position to adjust the speed to 3 rpm. The scale value is then stabilized and the shear stress value is read from the dial and recorded as SWV 3 rpm dial reading (DR). For 0.3-rpm readings, the viscosity is measured using a Brookfield viscometer (Brookfield LV DV-II or DV-II viscometer with LV-2C spindle). The speed of the spindle is set to 0.3 rpm, the spindle is rotated for 6 minutes or more, and the viscosity is recorded as the SWV-0.3 rpm scale value and expressed in centipoise (cP). For cement applications, the PEG LSRV test (testing low shear viscosity using polyethylene glycol as a dispersant as outlined below) provides an indication of the effectiveness of the performance of viscosity modifiers for the industry. This test measures the viscosity of a 0.25% solution of biogum in Standard Tap Water (STW). STW is prepared by adding 10.0 grams of NaCl and 1.47 grams of CaCl ₂ .2H ₂ O to 10 liters of deionized water. For viscosity measurements, 0.75 grams of biogum is added to 4.5 grams polyethylene glycol 200 (CAS 25322-68-3) in a 400 ml beaker and fully dispersed. 299 grams of STW are then added to the beaker and mixed for approximately 4 hours using a low-pitched propeller stirrer at 800 ± 20 rpm. After 4 hours of mixing, the beaker was placed in a 25 ° C. water bath and allowed to stand for approximately 30 minutes. The viscosity is then measured using a Brookfield LV viscometer with a 2.5+ torque spring (or equivalent instrument, such as Model DVE 2.5+) at 3 rpm (first rotating the spindle for 3 minutes) using the LV 1 spindle. This is referred to as centipoise (cP).

The produced diutan sample was tested in this manner; The results were as follows:

From plasmid-containing strains Diutan Rheology Strain SWV 3 rpm

DR SWV -0.3 rpm

cP PEG LSRV

cP Conduct # 1 Conduct # 2 Conduct # 3 Conduct # 1 Conduct # 2 Conduct # 1 Conduct # 2 Conduct # 3  S657 Wild Type 25 26 22 24400 28600 2820 3150 2280  S657 / pS8 42 43 47 41500 38800 4720 4980 4920  S657 / pX6 25 29 26 25000 29100 2860 3400 3270  S657 / pS6 --- --- 22 --- --- --- --- 2270  S657 / pX4 --- --- 24.5 --- --- --- --- 2950 SWV = viscosity in seawater
LSRV = low shear rate viscosity

Surprisingly, there is a marked increase in viscosity exhibited by the diutan gum according to the invention produced by some genetically engineered plasmid containing strains. But most surprisingly, the viscosity increase of SWV at 3 rpm for the pS8 strain was 80%, while the same assay conducted for the pX6 strain was only 9.6% compared to the wild type results. There was no significant increase in plasmids pS6 and pX4. Likewise, a low SWV rpm test showed an increase of 51.5% for wild type for pS8 type, whereas an increase of only more than 2% for pX6. Finally, the polyethylene glycol LSRV test showed a pS8 result of more than 77% increase in viscosity compared to wild-type gum, compared to less than 16% increase for pX6 diutan, 7.2% increase for pX4 and significant increase for plasmid pS6. Showed that there was no. In addition, a very surprising result during this period is that diutan gum production is greatly improved by using the necessary gene sequences exemplified in the pS8 plasmid as a way of introducing sequences in the target diutan producing bacteria.

Thus, the diutan according to the invention produced through the introduction of pS8 showed a surprisingly increased viscosity measurement in all three calculations, especially compared to the variants produced by wild type and pX6 plasmids. Thus, it was expected that this new diutane would work very well under typical dielectric conditions and in cement applications.

Rheology  Basic description of the improvement

The above examples showed that Diutan from S657 / pS8 strain showed a significant increase in rheological parameters. Thus, this substantial increase in seawater and PEG low shear viscosity measurements cannot be seen solely due to increased productivity, since the pX6 strain also showed similar (if not higher) yield results. In addition, in the previous examples illustrated in Table 2, the dry weight yield (alcohol precipitable) increased to 8.0%, while the rheological parameters increased significantly more for the S657 / pS8 strain (52-80). %). Basic studies were performed to demonstrate that rheological improvements were obtained using strain S657 / pS8 in wild type strains.

Intrinsic viscosity is a well known technique in polymer science for estimating the molecular weight of large molecules (C. Tanford, 1961. Physical Chemistry of Macromolecules . John Wiley & Sons, New York). Intrinsic viscosity is obtained by plotting the reduced viscosity (normalized viscosity to concentration) versus the concentration of the solution, and extrapolating the linear regression of the data to the zero concentration (y-intercept of the plot). Surprisingly, the produced black showed an increase in intrinsic viscosity as shown in the table below.

Five diutan samples, namely two samples from the wild-type strain (Control 1, Control 2) and three samples from the S657 / pS8 strain (Sample 1, Sample 2, Sample 3), were subjected to intrinsic viscosity, neutral sugar and organic acid. The analysis was evaluated. These samples were purified by alcohol precipitation, rehydration, treatment with hypochlorite, treatment with glucoamylase, treatment with lysozyme, and finally treatment with protease (performed in sequential order). These were then recovered in a 4: 1 CBM: broth ratio, dried and ground. CBM is an azeotropic isopropyl alcohol / water mixture comprising up to 82% by weight isopropyl alcohol.

Samples were tested for water content by performing as follows: In general, two 0.7 gram aliquots of the samples were tested using a Mettler HB 43 halogen moisture balance. The results from the two tests were then averaged and the results were used for moisture control.

After obtaining moisture data, a 0.2% solution of gum was prepared in 0.01 M NaCl based on controlled moisture. For three tests, a total of 200 grams of 0.2% solution was prepared. The gum was weighed on an analytical balance to the maximum approximation of the third decimal place and added to the water weighed to the maximum approximation to the third decimal place. Samples were stirred for 2 hours using a 2.5 inch diameter propeller mixer at 1000 rpm in a 400 ml long beaker.

Following initial hydration, each sample was diluted to 0.02% with 0.01 M NaCl. This was done by weighing 20 grams of 0.2% solution into a 400 ml beaker and then adding 180 ml of diluent as well. The diluted sample was mixed for an additional 30 minutes. The final dilution last used to measure the intrinsic viscosity was prepared from this sample. Each diutan sample was evaluated at concentrations of 0.004%, 0.008%, 0.010% and 0.012%.

Viscosity measurements were performed using the VilasticA® VE System. Prior to the measurement, Vilastic was adjusted to an error of less than 2.0% with water. Samples were measured using a Timer program (running at 2 Hz), one strain and a shear rate of approximately 12 1 / sec (all at a constant temperature of 23 ° C.). The measurements were taken five times and averaged for each sample. The averaged viscosity data was then used to calculate the intrinsic viscosity. 2 and Table 4 below provide the final results of the tests.

Comparison of Diutans Based on Intrinsic Viscosity Calculations Diutan
Sample Measured
solid inherence
Viscosity S657 control group 1 93.76 138.3 S657 control group 2 92.42 143 S657 / pS8 Sample 1 91.7 170.7 S657 / pS8 Sample 2 91.4 162.2 S657 / pS8 Sample 3 91.94 162.8

The results show that the S657 / pS8 strain consistently produces diutan with significantly higher intrinsic viscosity, in fact the average decreasing viscosity of the strain according to the invention was 165.2, whereas the control group was 140.7 (all similar measured solids). Level). This result shows that the diutane produced by S657 / pS8 has a higher molecular weight than the wild type control.

FIG. 2 is a graph of this trend showing a constant and high intrinsic viscosity measured with similar solids content between the control and the strain according to the invention.

In order to confirm that the high viscosity diutan gum derived from S657 / pS8 has the same composition as the diutan derived from the wild type strain, the composition was measured by testing for neutral sugars and organic acids. Purified samples used for intrinsic viscosity measurements were used for neutral sugar analysis. Aliquots of each purified sample were hydrolyzed to constituent sugars using trifluoroacetic acid (100 ° C./-18 h). The hydrolyzate neutral sugar was quantified by high performance anion exchange chromatography using pulsed current detection. The hydrolyzate organic acid was quantified by high performance ion exclusion chromatography using chemically inhibited conductivity detection. Table 5 below summarizes the results from the neutral sugar analysis. As can be seen, the neutral sugar profile for the S657 / pS8 strain is nearly identical to the neutral sugar profile for the S657 wild type strain. Although both results differ from the theoretical values, these results show that the structure of the repeating unit of the diutan gum prepared using pS8 is identical to that of the wild type, and any increase in the viscosity imparted by the pS8 material is long chain, i.e. Due to high molecular weight.

Neutral sugar and organic acid analysis for pS8 and wild type (control) Diutan strains Strain % Rhamnos % Glucose % Acetate Sample 1 S657 / pS8 32 19 8.9 Sample 2 S657 / pS8 32 19 8.2 Sample 3 S657 / pS8 32 17 8.6 Control 1 S657 Wild Type 30 18 8.6 Control 1 S657 Wild Type 33 20 8.7 Average S657 / pS8 32 18.3 8.6 Average S657 Wild Type 31.5 19 8.65 Theoretical value --- 46 30 8

Thus, the significantly improved seawater viscosity and PEG low shear viscosity of the diutan produced by the S657 / pS8 genetically engineered strain is due to the increase in molecular weight or length of the diutan molecule, ie the presence of more repeat units per molecule. It is not due to changes in the utane molecular composition and thus to the change in the repeating structure itself. Improved rheology may not be due to only an increase in the amount of diutan produced. Some of the bundles of genes for cloned diutan biosynthesis evaluated four different plasmids, pS6, pS8, pX4 and pX6, all of which showed a slight increase in productivity, with only plasmid pS8 recovered. There was a surprisingly high increase in the rheological parameters of.

Comparing the genes for cloned diutan biosynthesis in the tested plasmids suggests that the gene responsible for the increased molecular weight is likely the gene dpsG , because this gene is present in pS8 and not in other plasmids. . The gene dpsG encodes a hydrophobic membrane protein with strong homology with other membrane proteins involved in polysaccharide synthesis. Some proteins have homology with proteins for polymerases, enzymes that promote the binding of repeat units to form high molecular weight polysaccharides. The homologous gene gelG in S60 was considered to act as a polymerase for gellan synthesis (Harding, NE et al. 2004. "Organization of genes required for gellan polymerase biosynthesis in Sphingomonas) elodea ATCC31461 "J. Ind Microbiol Biotech 31 :...... 70-82 Sa-Correia, I. et al 2002." Gellan gum biosynthesis in Sphingomonas paucimobilis ATCC 31461:. Genes, enzymes and exopolysaccharide production engineering "J. Ind . Microbiol . Biotechnol . 29 : 170-176.) Homologues of dpsG were also isolated from sphingomonas strains ATCC 31554 and ATCC 21423 producing polysaccharides S88 and S7 (Pollock et al., US Pat. No. 5,854,034, No. 5,985,623 and 6,284,516, and Pollock, TJ US Pat. No. 6,709,845. Thus, it is likely that additional copies of the gene for polymerase will affect increasing the molecular length of the diutan molecule. It cannot be excluded that other genes in the bundle may be needed with dpsG to achieve the observed increase in viscosity, as candidates for genes encoding sugar transferases I, II, III and IV dpsB , dpsL , dpsK and dpsQ, special Is the first party of the repeating unit may be a gene dpsB encoding a transferase I to be added to the lipid carrier. Other important gene, when it is amplified by the multi-copy plasmid gene it has been found to increase the xanthan molecular gumB and gumC Homologous to dpsD , dpsC and dpsE All genes cloned in plasmid pS8 may be required to achieve dramatic viscosity increases.

While the present invention has been described and described in connection with certain preferred embodiments and examples, it is in no way intended to limit the invention to those specific embodiments, but rather to the extent of the appended claims and their equivalents. It is intended to cover structural equivalents as may be defined and all alternative embodiments and modifications.

submission

The following bacterial strains were obtained from the American Type Culture Collection of the Boulevard 10801, Manassas University, Virginia, USA, dated October 21, 2005, in accordance with the Budapest Treaty for International Approval of Microbial Deposits. Deposited to Patent Depository:

Sphingmonas strain S657 containing plasmid pS8.

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SEQUENCE LISTING <110> Harding, Nancy       Patel, Yamini <120> HIGH VISCOSITY DIUTAN GUMS    <130> 05-506, 05-506A <160> 45 <170> FastSEQ for Windows Version 4.0 <210> 1 <211> 26278 <212> DNA <213> Sphingomonas sp. ATCC53159 <400> 1 gatcaacggc gccttgctcg gacggcacaa attcgtcctg gtcaatgtgt ccacggtcgc 60 ctcttcgata ctgttccagc tgttcccgct tgtcgtcgcc tggatgatcg gcccggacct 120 gcgaacgctg ctgatcgccg cgctcgttgg ccgggcggtt ccgatgatcg gcatgctgcc 180 cgcgctgtat cgaaaccttt tgcgcggcaa cacgccgcgt tttcacgcca gcgaggcgcg 240 cttcctgata ggctatggcg ggtgggcctc gctcacgacc gtggtagcga ccgtgctcat 300 gatggcggac cgcttcctga ttggcgcact tcttgggccc gtcgccgtga ccatctacac 360 ggcccccctg caactcgcac agcgcgtatc gctgctgccc tccgcactgt ccgccgcgct 420 gttcccgcgc ctgcccagcg cgacgccggc ggagcgcatg gcgcttcaga tccgctcgct 480 gtcgctgatc atgggcggcc ttaccgggat gatcggcggc ggactattgc tggccgcgcc 540 gtttctcgat ctctggatcg gcaagtcgct cggccatgcg ggaacgccgg tcgcgctctt 600 cctgttcttc ggcgcatggt ggaatgcgct ggcgatcatt tcgttcagcg gcctgcaggc 660 gagcggacgg ccgaaagcga gcgcgatcgt ccagggggca gagctgctac ccgtgttgat 720 cgcgctgtat gcagggatcc gatggggcgg cgtgaccggc gccgcagcgg tctttctggg 780 acgctccgcc ctggatttcg tcctgttgac ctggcaggca ggcctgctcc gccagacggt 840 gaagcaagta tccgtatgcg gcgccgttct caccgtcgcg atgctcgtgg gcgcgaccta 900 tcgctattcg gtgccgctct ggtgcgtact cagcgcctgc tgcctggtcg cgctggcagc 960 ctgctcctgg tggacattgg cgcgccagga caaggcactg ctgattggac gattgagccg 1020 aattctacca aagcagcggc aactcgacct atagcctttc cgcaatgcac cgatggacca 1080 caccaacccg ttttaattga cacacacaaa tgctacaccg acaaagacac aggccgagag 1140 cgatatagaa gcgctatgcc tagccccagc gtcataaaga tgaacgggtc attgtcacct 1200 tgcgacagga ctgaccgcgt atttaaaaga acagccagga aagttgctac ggcgagctca 1260 agcgggtagc catctccgct catcttaaga ccacgaaacg cgagcaaaat cattaacgta 1320 atcatcgtgc cgtatagcga aacaaaaccc agcaagccgt aatcagccgc tacggacagg 1380 aaaccactgt cgatcgatag gaagccttgc tgattacgcc acccgacagc gccagcaccc 1440 tctcccgggc catagccgaa gaaagggcgg cgagcgatgg caggcacgcc caagcgaaac 1500 tgctcctgcc tgccttgatt gctaagttga gaagcgcctc caccgagaac acggttgtgg 1560 acggcaggca cgaacatgac cgccagcgac agcgccacca tcaaggcggg atacgtcaac 1620 gtcagcgaaa tgccgacaag cccgcccttt gtggtccgcc accgccgaat tgcccaaata 1680 agcaaataca cggtatgcgc caccaatccc cccaccattg ccagtcgaga accgctaaga 1740 aatccggacg caactacaag aaaatcgaag aaaatccaaa atgccaatct ccctacgcca 1800 cgggaattcg ctatacggtg cagcacgaaa ggaatcgtca aagccgtcaa ctctccccag 1860 acaagcggac tgctgaaagt cgtcaaaacg cggtaagtac cccggaaacc gggcgtaagc 1920 actacggtaa gaaactgctc atcaacgcgc aggaagctcg gaatcgagta ggcccagagg 1980 acgtgcttca cccggaactc cagcacgcca atcgccatca gcacgcccac gcaccaaaac 2040 aagcgcgtaa cccaccactc cggggtgcgc gtgtcggtcc cgatcagcca tagcgagatg 2100 aatgccatcg gcgtcaccgt cagaacgatg ccaatcaacc gcggaattgt ttgcgaggcc 2160 gctggggtcg caatggaggc gacgatctgg accataatga aggcaagcaa tagtcgcgat 2220 gggatcggcg ccgcccgcat aatcgccgcc atctcggatc gaaacttttt cgagaccgaa 2280 agcgagatca tgagcgtgag caatgcgatc gaaccgatca tccgcctgat cgagatccaa 2340 ggcaaaccac caacgctgag cgcaagatag ttcggccaca cgagcgccgc caccatatag 2400 gcgaggtata gttttgccag caggcgagta ggcgcctgcc gcgcctcggg tagcgcccag 2460 atcactacga gcgccatcag aacgaggggc acggccggga tcgccagcat ctggagcggc 2520 agaactgcgg cgagcaggcc gtagactgcg gcaagaaaca tcacgctgac cagcagaacg 2580 gtacgccgcg ccgcgatcgt cacgcctgat cgctcggctt tgtagacggg cagtaccggg 2640 atcgctggct ttgtcagaaa ccgaaccagt cgcaacctgc gaagccgctg catcgctccg 2700 tggaaggccg ggcgacgaaa cgccgaggta gtcgtcatct gcaagtcccc aacaagtccc 2760 caagaggcgc tgccgctcgc atgatcgaag ggttcgcgaa aagcaaggtc gatacgccgc 2820 actccctgcg atgtgccgcc ggatcgcagg agggcacggg cggcgccggc gcaaggccgc 2880 tcaccgcccg cccccgctca ggcgcggtac aggttgtact gatccgccgt agcgctcagt 2940 gtcgccgcgc tgcggattgc gcccatcgcc cccgcggtca tcatgtcgac accgatcttg 3000 ctgacgagcg cgatctgcga ggacgcggca gtacctatag acagcgtact gcccaccgtg 3060 gccaccgtcg caagcggcgt tgccgtgcta gcggcgccgg cacccgccag cagcgcagcg 3120 gcctgcgcgg ccgcgccggt gacgaggctg tccttgaccg tcgccgccgc gctggcgctc 3180 gacgcggtca ccagcgcctg cacctgggcg gcgctgatcg cgccatcgcg gatctcgatg 3240 tcgccgaccg ttccgctgaa tgcggtcgag aacgggctgc cgacatacag cccccaggat 3300 tcggcgggcc gggtcgtgcc ggtcatcgtc gctgtgccgc gttgcatgcc gtctacgtac 3360 agaatcgcgg ttttccgcgt gctgtcgtag gtcagggcga tcttgtgtgt ggcagcatca 3420 agcagcttgg cgccgctcgt caccattgtc tggctgacgc ccgcggcgtt gcgcatggtg 3480 aagctcagtt ccccattggc ctgcagcgaa accgaccagc tctggaagat gccaagaatt 3540 tgcccggccg tggccgtagc cgagtcccgc ttgaggtcga agctgagcgt gaacgccgac 3600 aatgcgtaaa tctgccgcga atagctccgg tttagttcca cccccgtgcc cgtcgagacg 3660 tggaaggcgc tgcccacgac cgccgacacg tccaccgcct ttgtcgtctg gccggtattc 3720 cagtgcgaaa ggtccacgac gccgctgttg ctgaacgaca gatcgagcag cagcgacgga 3780 tttgccgcct tcgcagtcga cagttcggta gtcacctgag cggcagcagc gctcgacacg 3840 ggcggctggt acccgacgcc gggaacgatc aaatcgctga gccgcgccgt agccccatcg 3900 ttgaggccat agatcttgcg gatcgttgcc gagtcactcg tcagcgtacg attgcctgtc 3960 tgcacgatat tgctcgagga gcttgtgacg gtgatcaggt ccgcaacatt gttcttgatc 4020 gtcgcgccat tggttttgtc gaggcgaatc caaaatgatg tgccatccac ttgcgatatc 4080 acgctattgg attcgatatt gacattaacg ccgttaacaa cgttgatacc gtggtaataa 4140 ccattcagat agataagatt gtttttgatg tttacattga catagggaag attaccggcc 4200 tcgtcattca tgaaaatccc ttgcgcgcca gagcccgcgc cctgcatgat gacgttattg 4260 gagatggtga tgttggtatt gcccttgacc ttgcccgccg tgaagaactg aatggcgtcg 4320 ggatgttcgg tgcccacggg aaacaggttc gtgaacgaat ttccgtcgat gacaagattg 4380 ttcatctcag tgaagttcgt atgatcgcgc cggttgtcgt ggaagctgct gttctggacc 4440 accatgccat cgacgttgta ggcctcaagg cccagaccga agtggtcgat agacgaattc 4500 tgcatcgtca ccgacgtgct gttgcgcacg aacaagcccg cccccttcga gagcgaaggg 4560 tcaccagtgc cgccgctgaa ccgcacgccg tccaaaacga tgttggccga accctggatc 4620 gtattcagtc gattccagtc atcggcgggc ttgtaatcgg tcgcagcgac catgtttttg 4680 acggtaacgt tgctactgtt cccgatcacc agcttttgga tattgaccgg gttcgacgag 4740 tcgagcgact caattgtcac catgctggta aacgtcttgg tcattacagt gagatctgtg 4800 tagaccccgg cggcaagctt gatggtttcg ccacccttcg ccgccgcgat tgcagcattc 4860 aactccgtct gattcttgac aatgatatcc ggcatgttga cttaccccgt acgcacgaac 4920 ccgggccgat attgaccctt ccattgtcat aaataccaga acagccatga aatttgctcg 4980 aagggataca gttaagaact cccttctacg gggccgcatg ccgggcccat gcacgcccga 5040 ctttcgccgg caccgtctcg acggcgcaac acagtgcagc tactagggtg cgatgcagat 5100 gctcccaacg cccgatgtca gcatactcgt ggtcgctttc aactcgaccg agtatatcga 5160 agactgcctg cgcggcatcg ccgaaggagc gggcaagacc ccccacgaag ttctgctgat 5220 cgacaatggc gacgggcgaa ccgaagcgct ggtccggcag cggttccacc acgtccgcat 5280 cgttcccagt gagggcaata ttggtttcgg ggccggcaat aatcgcctgg cagcgcaggc 5340 tgccggcccg ctcctgctgc tcgtcaaccc cgatgccatt ccccagcccg gcgcaatcga 5400 tcagttggtc acctttgcca aacagcatcc cgaggcggcg gcatggggcg gccgttccta 5460 ctcgcccagc ggcgatctag aacccgcaaa tttcatgtcc ctgccgacgc ccgccgactt 5520 tctgacggcg attttcaacg cgcgtgcgct acgcagcggc gggctgcaag aaggcgcgac 5580 cacccccgga gcggtcgagg tgttgaatgg cggcttcatg atggtacgca ccgatgtctg 5640 gcaggcgatc ggcggttttg acgagagctt ttttctttat tcggaagaga tcgatctctt 5700 ccagcgaatc cgcacgttgg ggcacaaggt gctcgtcgac ccctcggtca aagtggtaca 5760 caatacgggg agtggtcagt cgatgtccca gaaccgcctg atgtatctca cgaccgggcg 5820 catgcactat gcgcgaaagc attttggcgc actcggcacc cttgccaccg ggtgcgcgct 5880 ttggctgatc gccgccaaat acacgttggt cggggcggca ctctggcgcc tgtcgccgcg 5940 gacgggcacg cgatacaaag agctgagcaa cgggtggcgt gccgtattta gcaatcctgg 6000 ccgatggtgg agcggctatc cgcgtcgcta aaagtccagc tccccccccc ctaaaggcgc 6060 cgttgggagg cggacgcatc gttgcaacaa cgcgcccgcc tttcagacct tcagttcccc 6120 gccggcgttg cgccgctgcc gcgaagctgc ggcggtgcgc tgtagccggc ctgatatttc 6180 acggtttccc gcgccttctt caggcggtcg ttgagctgtg cgtcagccgc cttgccgaag 6240 cgctcggtac gcagcccgct gagcgcgatc tcgcgcgcct ggtcggccgg caccggcagc 6300 accgtggtcg acgtgatgat attcgcggtc agtccctgct gggtcggcag gatgaacatc 6360 tcctgtgccg gcagcgacgc gatcttggca gcgatttccg gcggcagcgc agcggtgtcg 6420 atctgcgacg gcgcgcgacg gaactggaca ttgtccgccg agagcttggc ggttagctgg 6480 tccagcgtct tcagcggcgc gaattgcttg agctttgcgg ccgagctcgg cggagcgaag 6540 acgacctgat cgatcgcgta gatcttgcgc tgcgcgaacc gctccggatg cgcggcctga 6600 tatttctcga tctcggcatc ggtcggctgg gcgatgccgc cggcgatctt gtcgcgcagc 6660 atggcggtga ggatcagctc gtcggcccgg cgctcctgga tcaggaaggc aggcgtcttg 6720 tccagcttct gctcgcgggc gaccttggcg aggatcttgc gctcgatgat gcgctgcagc 6780 gccagctgct cggccagctt gcgatcggtc cccgggggta cctgggaggc ctgcagttcg 6840 gcattcagct cgaagacggt gatttcttcg ccatcgacgc tggcgaccac ctgccccttg 6900 tcgagcttgc cgcccttgcc gccacatccg gagacggcca gcgcggccgc agccaccgcc 6960 gtaaccaggt acaatttctt catgaagacc tccccgccgg cacggaattg cgcacggcac 7020 aaacttctac ttgaacctat tcggacgggc gggcatccgc aatagcgttg gcagtgcagc 7080 atggttctaa gcggagccag gcggcaacaa gggggacgag atggcagaag cgaacgcggt 7140 agatggaaag gcctccaagc cgctgaaaat gtgccttgca gcgtcgggcg gcggccatct 7200 ccggcaaatc ctcgatctgg aatcggtgtg gcgcgaacac gattatttct tcgttactga 7260 agataccgcg ctcggccgga gccttgccga aaaacatccc gtcgaactgg tggagcacta 7320 tgcgctcggc caggccaagc tgggccatcc cttgcgcatg ctgggcggcg catggcgcaa 7380 cctgcgccag agcctttcga tcctgcgccg gcacaagccg gatgtggtga tttccaccgg 7440 cgcgggcgca gtctatttca ccgcgctgct cgccaaactg tcgggcgcca agttcgtcca 7500 tatcgaaagc ttcgcgcgct tcgaccaccc gtctgccttc ggcaagatgg tgaagggcat 7560 cgcgacggtg acgatcgtcc agtcggcggc gctgaaagaa acctggcctg atgccgagct 7620 gttcgatccg ttccgcctgc tcgatacacc gcgcccgccc aagcaggcgc taatcttcgc 7680 gacggtcggc gccaccctgc ccttcccgcg gctggtgcag gcagtgctcg acctgaagcg 7740 cgccggcggg ctgccgggca agctgatcct gcaatatggc gaccaggacc tgcccgatcc 7800 cggcatcccc gacgtcgaga tccgccgtac catcccgttc gacgatctgc agctgctgct 7860 gcgcgatgcg gatatggtga tatgccacgg cggcaccgga tcgctggtca cggcgctgcg 7920 cgccggctgc cgggtcgtcg cctttccgcg ccgccacgat ctgggcgagc attatgacga 7980 tcaccaggaa gagatcgccc agaccttcgc cgaccggggc ctgctccagg cggtgcgcga 8040 cgagcgccag ctcggcgccg ctgtggaagc ggccaaggca accgagccgc agctggcgac 8100 caccgaccac acggccctcg cggcgcggct gcgccagctg ctggcgcagt ggagtgccaa 8160 gcgatgagca cgccccggat cagcgtcgtc atcccgcact ataacgatcc gcaatccttg 8220 cggctctgcc tggatgcgct ggagcggcag acgatcggtc gcgacgcgtt cgagatcatc 8280 gtcggcgaca acaattcgcc ctgtgggctc gcggcggtgg aggcggcggt cgccggacgt 8340 gcgcggatcg tgaccattct ggaaaagggg gcgggccccg cgcgcaacgg ggcggcagcc 8400 gcagcgcgtg gcgagatcct cgcctttacc gacagtgact gcgtggtgga gcccggctgg 8460 ctggcgggcg gcacgaccag ggtcgcgcct ggccgtttca tcggcgggca catgtatgtg 8520 cgcaagcccg aagggccgcc gaacggcgcc gaggcgctgg agatggcgct ggcgttcgac 8580 aatgaaggct atgtgcggcg cacccagttc acggtcaccg caaacctgtt cgtgatgcgc 8640 gccgatttcg aacgggtcgg cggcttccgc gttggcgtgt ccgaggatct ggaatggtgc 8700 caccgggcga tcgccagcgg cctcaccatc aactatgcac cggatgcatc ggtgggccac 8760 ccgccccggc ccgactggtc ggccctgctg gtgaagacgc ggcgcatcca gcgcgaactc 8820 tatctgttca acatcgagcg gccgaagggc aggctgcgct ggctggtccg ttccgtggcg 8880 caaccggcga tgatcccaca ggacgtggcc aagatcctgc gcacaccggg taccaagggc 8940 gcgcgcctcg ctgcggtcac cacgctggtc cggctgcggc tgtggcgcgg cggcgccggc 9000 ttgttgcagt tgctcggccg cgacatctga tcgaccggcg atcggccgac gagcgcgtcg 9060 ccggccgatc gcattgcatc agacggtggc cagcgcgtct tccagcgtgc cgctgtcgag 9120 ccgcaggcgg ccgatcatca gccacagata gaccggcagc gtatcgtcgg tgaagcggaa 9180 gcggcaatcg ccgtcctgcg tttcggattc gaggccgagt tgaccggtga gctcgcccag 9240 ctcctgctcg acctgcgccg ccgtgatgtg cgcgcccggc agcagatcca ccacggcttg 9300 gccgctgaac cagccatccg ccgagcgcga ggcctcgccc agcgccgcga cgagtggatc 9360 gtagcggccg ccgacgaact tgcgcatctc gatcaccgcg cgcggcggc tgcggccctc 9420 gatctcaagg atcgcctggt cgagcgcacg acgcagatgc ccggcgtcga ccgtgaggcg 9480 gccctggtcc agggcttcca gcgcggaatg gtggcacagc agccgcgcga aatagggcga 9540 ccccagcgcg agcaggtgga tcatgtgagt caggtccgga tcgaagcgaa cgcccgaggc 9600 ggtttcgccg agcgcgatca tctcctgcac ctccgattcc tccagccggg gcatcggcag 9660 gccgatgacg ttgcggcgga tcgacggcgc ataaccgatc agctcctgca ggttcgaggc 9720 gacgcccgcg atcaccagct ggacgcgcgc cgaacggtcc gacaggttct tgatcagctc 9780 ggcgacctgc tgacggaagg cggaatcgct gacgcgatca tattcgtcga ggatgatcag 9840 cacgcgtgtg cccgtgatgt cggcgcacag gtcggccagt tcgccgggcc cgaagctgcc 9900 cgtcggcagg cggtcggcca agttgccgcc gctctccgcc tcgccggcgt tgggcgccac 9960 gccgcgatgg aacagcagcg gcacgtcttc cagcacggcg cggaagacat cgctgaaatt 10020 cgcgttcgca ccgcaggtcg catagctgac gatatagctg gattcgcggg cgacatcggt 10080 cagcacgtgg agcagcgagg tcttgccgat gccgcgctcg ccatagagca cgacatggct 10140 gcgctggctc tcgatcgagg agattaggcg cgccagcacg ccgaggcgcc cggcgaagct 10200 cgaccgatcg gccaccggct gggtgggtgt gaagaaggtc gccagcgcga accgggcgcg 10260 cgtgatctcg cggcgctcgt cgcggcggcg atccagcggg cggtccagcg cggaggcacg 10320 gaaggttggg aaatccgggc gaccacggcc gctatgggca tcgcgatgcg gcaccactgt 10380 cgcagtcagc gggaaatagc cctcttcttc aggttcttct cgacggccga acggccacaa 10440 gaatctcagc gcggaaccta cagccactcg aacacctctt aaattcgtgc gccatcggca 10500 ccgacggcgc accctggttc gcgccccctg gcgccccctc ctaacgaacc cacgccttgc 10560 ctggcctatc ggcgcttgaa gaactcgtac ggtttgatca ccaaggcgat gtacgccagg 10620 accagagcga tcgtcaaaat tgcaaagacg tgataattct cattgcccag ataattggcg 10680 acggcgcaac cgactgcggg cggcaaatag ctgatcatcg tgtcccggac tgccgaatcg 10740 gcttgggacc gttgcaggaa tataacgatc aggccggcaa atatcgcgat ggtgacccaa 10800 tcatagggcg tctgcatgca tgtcctttct attcgacacc ggaatcgaac catttccggc 10860 gacgctattg cacgcactag cagtgcgcgc ggccgctcgc taggtagcgc cgcaccggat 10920 aaaccgacgt taagatggcg cggctcgatc gaaatggagt caaacgggct tgcccggccg 10980 accgaagcat ggcgccatgg cgcatgcacc gtattgtgac cacgcaaacc gcgagggtca 11040 ttcgatgcgg ttgcttgtac aggaggccat tgataatgaa gccgagaccc gggggaacct 11100 ttatgcaagt aaatttcaat cgacaggctc gcaagctcgg tgccggcaat gcgctcgcgc 11160 ggggggggcc cgtgcttgcg ctgcttgcga ccgcggcatg gacacaacct gcgctggcgc 11220 agcgacaggc atttgagtcc cgcccctccg gtagcgagcg acaggtcgat attcgcgcga 11280 cggggtcgct ggaatatgac gacaacgtcg tgctgaacga ccagcggatc acggacggcg 11340 cgcgtggcga tgtgatcgca tcgcccgggc tggacgtgac cctagttctg ccccgcgcca 11400 ccgggcagct ctacctcacc ggcaatgtcg gatatcgctt ttacaagcga tataccaact 11460 ttaaccgcga gcagatctcg ctcaccggcg gcgcagatca gcggttcgcc tcctgcgtcg 11520 tgcacgggga agtcggctat cagcgccacc tcaccgacct gtccagcatc ttgatccagg 11580 acaccacgcc tgcgctcaac aacaccgaag aggcccggca gtacaccgcg gatatcggct 11640 gcggcgcgac ctacggcctg cggcctgccg tttcctacac ccgcaacgaa gtgcgcaaca 11700 gccttgccga gcgccgatac gcggactcga ataccaacac ctttaccgca cagcttggcc 11760 tgacttcgcc tgccctgggg accgtggcgg tatttgggcg tatgtccgac agcagctatg 11820 tccatcgcgt ccttcccggc attaccggcc aggacgggat gaagagctac gcggccggcg 11880 tccagctcga gcgctcggtg gccaaccgac tccatttcaa cggctcggtg aattacaccg 11940 aggttgaccc aaagctcgca tccaccaaag gattcaaggg cgtaggattt aacgtttccg 12000 gcgattatgc tggtgatcag tacagcctcc aattgctggc ttcacgatcg ccccagcctt 12060 cacttcttct gttcgtgggt tacgagattg tgacagcggt ttcggcgaat gcgacgcgcc 12120 ggctgagcga tcgcattcag atatcgctgc aaggcagccg aacctggcgc gagctcgcgt 12180 cttcgcggct gctcaccaac gtgccgattt ccggcaacga caacacctcg acgttgttcg 12240 cctccgctac cttccggccg aatcgccggc tgagctttgt gctgggtgcc ggccttcagc 12300 ggcgcaccag caacacgcag ctatacagtt acagctccaa acgcatcaat ctctcgacgt 12360 cgctttcgct ctgacaaggg ccgtaatcat gcatatcaag aatcgcttcg tgaatatctc 12420 gacgttggcc atcgccgccg cgctggccac gccggcggcg gcgcagatcc ccacgcggtc 12480 cgtgcccgcg ccggcccgcc cgcggcctgc aacgccgccg gcgcaacagc agaaccaggc 12540 gccgtcgacg cccgcagcgg caaccccggc gcagaccgcc gcaaccgttg cccctgcagc 12600 aaccgcaccc gcaggttaca aaatcggcgt ggacgacgtg atcgaggccg acgtgctcgg 12660 ccagaccgac ttcaagacgc gcgcccgtgt gcaggcggac ggcacggtga ccctgcccta 12720 tctgggcgcc gtgcaggtca agggcgagac cgcgacctcg ctcgccgaaa agctggccgg 12780 gctgctgcgc gccggcggct attatgccaa gccgatcgtc agcgtcgaaa tcgtcggttt 12840 cgtcagcaac tatgtgacgg tgctgggcca ggtgaacagt tccggcctgc agccggtcga 12900 ccgcggctat cacgtttccg agatcatcgc ccgtgccggc ggcctgcgcc ccgaagcggc 12960 cgatttcgtc gttctcaccc gcgccgatgg ctccagcgcc aagctggact acaagaagct 13020 cgcccaaggt ggccccaatg acgatccgat ggtgacgccc ggggacaagg tctttgtccc 13080 ggaagtcgag catttctaca tttatggtca aattaacgcg cctggcgtat acgcgattcg 13140 atcggacatg acgctccgtc gcgcgctggc ccagggcggt gggcttgccc ccgcaggctc 13200 cgtcaagcgt gtgaaggtca cgcgggatgg caatgaactc aagttgaagc tggacgatcc 13260 gattctccca ggcgacacga tcgtcatcgg cgaacgattg ttctgatctt ggcaacgatg 13320 gcagcggacg aggcccacca gtgaatatca ttcagttctt ccgcattctg tgggtgcgcc 13380 gatggatcat cctcccggcg tttctcgttt gcgttaccac tgccaccatt gtggtccagt 13440 ttctgcccga acgctacaag gccactacgc gggtggtgct cgacacgttt aagcccgatc 13500 ccgtcaccgg acaggtgatg agctcgcagt tcatgcgcgc ctatgtcgag actcagaccc 13560 agctgatcga ggactatgcg accgccggtc gcgtggtcga cgaactgggc tgggtgaatg 13620 atccggcgaa catctccgcg ttcaacaact cgtccgcggc tgccaccggc gacatccgcc 13680 gctggctcgc caagcagatc atcgacaata ccaaggccga tgtgatggag gggagcaaca 13740 tcctcgaaat cacctattcg gacagctcgc ccgagcgcgc cgaacgcatc gccaacctga 13800 tccgcacctc gttcctcgcc cagtcgctcg ccgccaagcg ccaggccgcg accaagtcgg 13860 ccgactggta cgcccagcag gccgaagctg cccgcgattc gctcgctgcg gcggtccagg 13920 cccgcaccga tttcgtgaag aagaccggca tcgtgctgac cgaaaccggc gccgacctgg 13980 aaacccagaa gctccagcag atcgaggggc agacgacgac cgccaccgcc ccggttgcca 14040 tggcccccag cggcatgggc ccggcgcaga tgcagctcgc ccagatcgac cagcagatcc 14100 agcaggcagc gaccagccta ggtccgaacc acccaacttt ccaggccttg cagcggcagc 14160 gcgaagtgtt cgccaaggca gcggcggcgg aacgcgcgca ggcgaacggc gtatccggtc 14220 cggcacgcgg ggccatcgaa agcgcagcca acgcccagcg cgcgcgggtt ctcggcaatc 14280 gtcaggatgt cgacaagctt acgcagctgc agcgtgacgt ctcgctgaag caggatcagt 14340 acatgaaggc ggcacagcgc gtcgccgatc tgcggctgga agcaagcagc aacgatgtcg 14400 gcatgtcgac gctcagcgaa gcatcggcgc cggaaacgcc ctattacccc aaggtgccgc 14460 tcatcatcgg tggtgcagcc ggcttcggcc tcgggctcgg tctgctggtc gcgctgctcg 14520 tcgagctgct cggccgccgc gtccgcagcc ccgaggatct ggaagttgcg atcgatgcac 14580 cggtgctggg cgtgatccag agccgcgcct cgcttgccgc ccgccttcgc cgcgcccaag 14640 aaaccctcgg cgaaggtgcc gacacgcacg gagcttcagt aaactgatgg acgcgatgac 14700 cagcgaaccg ctgcccgaag gcgatcgtcc gagcgccgtg ccgaccacgc cggatacgat 14760 cggcatgctc gaataccagc tcgtcctctc cgatccgacc gggatcgagg cggaagcgat 14820 ccgcgcgcta cgcacgcgca tcatgaccca gcacctccgc gagggccggc gcgcgctcgc 14880 gatctgcgcc gcctcggcgg gatccggctg cagcttcacc gccgtcaatc tggcgacggc 14940 gctggcgcag atcggcgtta agactgcgct ggtcgatgcc aatctgcgcg atcccagcat 15000 cggcgcagcc ttcggcctcg ccgccgacaa gcccggcctg gccgattatc tcgcctcggg 15060 cgatgtcgac ctcgcctcga tcatccatgc gacccgcctc gaccagctct cgatcatccc 15120 ggccgggcat gtcgagcaca gcccgcagga actgctcgcg tccgaacagt tccatgatct 15180 ggcgacgcag ctgctgcgcg agttcgacat cacgatcttc gacaccacgg cgtccaacac 15240 ctgcgccgac gcgcagcgtg tcgcgcatat cgccggctat gcgatcatcg tggcgcgcaa 15300 ggatgcgagc tacatccgcg acgtgaacac gctcagccgc acgctgcgtg cagaccgcac 15360 caacgtcatc ggctgcgtac tgaacggcta ttgatttgga ccatatggca gcgaccgcga 15420 tgacgcggca gcaggagagg aagggcggtg gctattggct ggccgttgcc ggtcttgccg 15480 cgctaaccat cccgaccttc atcaccctgg gtcgcgaggt ttggagtgcg gaaggcggcg 15540 tgcagggtcc gatcgtgctc gccacgggcg cctggatgct ggcccgccag tgctcgacga 15600 tcgaggcgct acgccgcccc ggcagcgtgc tgctcggcgc gctgttcctg ctggcgacgc 15660 ttgccttcta caccgttgga cgggtgttcg acttcatcag tgtcgaaacc ttcggactgg 15720 tcgcgaccta tctggtcgtc gcctatctct atttcggtgc cagggtgctc cgtgccgcct 15780 ggttcccggt gctgtggctg ttcttcctgg tgccgccgcc cggctgggcc gtcgaccgca 15840 tcaccgcacc gctcaaggag ttcgtctcct atgcggcaac gggcctgctt tcctgggtgg 15900 attatccgat cctgcgccag ggcgtgacac tgttcgtcgg cccctatcag ctgctcgtcg 15960 aagatgcctg ttcgggtctg cgctcgctgt ccagcctggt cgtcgtgacg ctgctctaca 16020 tctacatcaa gaacaagccg tcctggcgct acgcggcgtt catcgcagcg ctggtgatcc 16080 cggtggcagt ggtgaccaac gtcctgcgga tcatcatcct ggtactgatc acctatcatc 16140 tgggcgacga ggcggcgcag agcttcctcc acgtctccac cggcatggtg atgttcgtgg 16200 tcgccctgct ttgcatcttc gcgatcgact gggtggtcga gcaacttctt ctcctgcgtc 16260 ggaggcatca tgttcaaccg gcgtgacctg ctgatcggcg caggctgctt cgccgccgct 16320 ggcgcctcgc tcggcctgaa gccgcaccgg cggatggacc tgctgggcgg caccaagctc 16380 gacacgctga tgcccaaggc attcggcgca tggaaggcag aggataccgg ttcgctgatc 16440 gcgccggcgc gcgaaggcag cctggaggac aagctctaca accaggtggt cacccgcgcc 16500 ttctcccgcg cggacggtgc ccaagtgatg ctgctgatcg cctatggcaa cgcccagacc 16560 gatctactgc agctgcaccg gccggaaata tgctacccgt tcttcggctt caccgtggtg 16620 gaaagccatg agcagaccat cccggtgacg ccgcaggtga cgatccccgg tcgcgcgctg 16680 accgccacca acttcaaccg caccgagcag atcctctact ggacccgcgt cggcgaatat 16740 ctgccgcaga acggcaatca gcagatgctc gcgcggctga agagccaggt ccagggctgg 16800 atcgtcgacg gtgtgctggt gcgcatctcg acggtgacgc ccgaggcgga agatggcctg 16860 agcgccaatc tcgatttcgc gcgcgagctg gtgaagacgc tcgacccgcg cgtgctgcgc 16920 ccgctgctcg ggaacgggct cacacggcag ctcggtcacc aggtctgaac cggtgcgccg 16980 cacgcggcgc ccccggcaac aaaaaaggag cggcgcgggc cgccgccgct ccctctcctt 17040 ctcatgcggc gccctgccct caccgctcgt gcagcgcgtc actccccgtc tcgagcacgg 17100 gccccaccag atagctgaac agggttcgct tgccggtgac gatgtccgcg ctcgcgagca 17160 tccccggccg cagcggcacc tgtgcgccat gggccagcac atacccgcgc gccagcgcga 17220 tccgcgcctt gtagaccggc ggctggttct ccttcatctg caccgcctcg gggctgatgc 17280 ccgccaccgt gccgggaatc atgccgtagc gggtataggg aaaggcctgc agcttcacct 17340 ttaccggcat gccgatgtgg acgaagccga tgtcgctgtt gtcgaccatc acctcggcct 17400 cgagccgggc attgtcggga accaggctga ggagcggctt ggccccttcc accacgccgc 17460 cttcggtgtg gacctgcagc tgcgagacgg taccgctcac cggcgcgcgc agttcgcgga 17520 acgagctgcg cagattcgcc ttggcgacgt cctcgccgcg ggcacgcacc tcgtcctgcg 17580 ccttgaccag atcctgcagc acctgcgccc gcgcctcctc gcgcgtcttg gccgacaggc 17640 tggagacgct cagcgactgc tggccgagtt tggcgagcgt agcgcgcgcc gccgtcaggt 17700 cctgccgctc ggcgatcagc tggcgacgca tctccacgac gcgcagcttc gagacatagc 17760 ccttggcggc catcgtctcg ttcgcggcga tctgctgttc gagcagcggc agcgactgtt 17820 cgagcttccg cacctgtgcc tgcgcctcgg ccgcggccga gacggcggca ccgcgatcgg 17880 agcggccgcc ggccagcgcc gcctcgatct ggcccagccg ggcgcgggcg aggccgcgat 17940 gcgtcgccac ttcgcccggg ctggcggcgg caggcgcgac gaagcggaag cccctgccgt 18000 ccagcgcgtc gatgatcgcc tggttgcgtg cggcgtcgag ctgggcgctg agcagcgcca 18060 ccttcgcctg tgccgcctcc gccgacgaca cggtcgggtc gagcgtgatc agcacctggc 18120 ccttggcgac cttctgcccc tcgcccacca ggatgcggcg gacgatcccc gattcgggcg 18180 actggacgat cttggtctcg ccgatcggcg cgatccgccc ctgcgtcggc gcgacgactt 18240 cgaccttgcc gatcgccagc caggcggcgg tgatcgccag cccggccagc atcaccttgg 18300 cggtaagccg cgcggtgggc gaaaccggcc gctcgatgat ctccagcgcg gcaggcagga 18360 aggcggtgtc ataagcgtcg acgcgggcag gcagcacggt atcgcgcatg cgggcgagcg 18420 ggccgccgcg gcgcatcgga acaacggcgt tcatgcggca atctccccat agccgccctg 18 480 gcggcggtgc aggtcggcat agcggccgcc caggcgcaac aattcgtcgt gtcggccgct 18540 ctcgacgatg cggccctgtt cgagcgtgat gatccggtcg cagctgcgca ccgcgctcag 18600 gcgatgcgcg atcaccacga gcgtgcggcc ggccgagatg gcgcgcaggt tgttctggat 18660 cagctcctcg ctctcggcat cgagcgccga ggtcgcttcg tcgaacacca ggatgcgcgg 18720 attgccgacg agcgcgcggg cgatggcgag ccgctggcgc tggccgccgg agagattgac 18780 gccgcgctcg acgatctcgg tgtcatagcc gcgcggctgg cgcaggatga aatcatgcgc 18840 gccggccagc gtcgccgccg cgacgacatt ctcgaacggc atggcggggt tggagagcgc 18900 gatgttctcg cggatcgagc ggctgaacag cagattctcc tgcagcacga cgccgatctg 18960 gcgacgcagc caggcgggat cgagctgcgc cacgtcgacc tcgtcgacca gcacgcggcc 19020 gagattcggc aggttgagcc gctggagcag cttggccagc gtcgacttgc ccgagcccga 19080 cgaaccgacg atgccgagcg aggtgcccgc cggaatgtcg agcgtgatgt cgctcagcac 19140 cggcggctgg tcctcggcat agcggaagct gacattctcg aagcgaatcg caccgcgcag 19200 caccggcagc gtcgccgccg aggccgggcg cggttccacc ggatggttga gcacgtcgcc 19260 cagccgctcg accgagatgc gcacctgctg gaaatcctgc cacagctgcg ccatgcggat 19320 caccggcccg gacacgcgct gggcgaacat gttgaacgcc accagcgcgc ctacgctcat 19380 cgcgccgccg atcaccgcct tggcgccgaa gaacaggatc gccgcgaagc tcagcttcga 19440 gatcagctcg atcgcctggc tgccggtgtt ggcggtattg atcagccgct gcgacgcggc 19500 ggtatgggcg gcgagctggc gctcccagcg attctgccag tgcggctcga ccgcggtcgc 19560 cttgatcgtg tggatgcccg agacgctctc gacgagcagc gcgttgctgg cggagctctt 19620 ctcgaacttg tcctccaccc gcgcgcggag cggcccggcg acgctgaacg atacgatcgc 19680 ataggcgatc agcgacacga gcacgatgcc cgagagcatc ggcgagtaga acagcatcgc 19740 ggcgaggaac acgaaggtga acagcgggtc caccatcacc gtcagcgagg cgctggtaag 19800 gaattcgcgg atcgtctcga gctggcggac gcgggtgacg gtgtcgccca cgcggcgctt 19860 ctcgaaatag gcgagcggca gcgccagcag gtggtggaac agccgggcac ccagctcgac 19920 gtcgatcttc tgcgtcgtct cggtgaacag gcgggtgcgg atccagccga gcgccacttc 19980 ccacaccgaa accgccagga aggcgaaggc gagcacgctc agcgtgctca tgctgttgtg 20040 gatcagcacc ttgtcgatca cgctctggaa caacagcggc gcggcgaggc cgagcaggtt 20100 gagcgcgagg gtgatgccga gcacctcgag gaacagcgtg cgatagcgcc ggaactgcgc 20160 ggtgaaccag gagaggccga accgcagcgg ccgtcccgcc accgcgcggg tggtgagcag 20220 caccagcgcg ccggaccaga tcgcgtccag cgcgtcccgg tcgacctgtt ccggggcatg 20280 gcccgggcgc tggatgatca cgccatgttc ggtcaggccg ccgatcacga accagccttc 20340 gggcccgtcg gcgatcgcgg gcagcggctg gcgggcgagt ccgccgcgcg gcacctcgac 20400 ggccttggcg cgcacgccct gctggcgctt ggccaggagg atcaggtcgt cggcgcttgc 20460 cgcctcggca tggcccagcg cgtggcgcag ctgttcgggc gtgatggcga tgttgtgcgc 20520 gccgagcagc agcgacaacg ccaccagtcc ggattcgcgc agctccgcct cgcgctccgc 20580 cgccccatgg gccgcgagcg cgctctgcag ggtggcctgc atttcgtcgc gtgtcatttc 20640 cggaactctg cctccatggc gatactgaga gcgccatgat gaagaaggct ggtaaagact 20700 cacttaatcc tagcttttct ggtatttacc cgtagctgcc gacccgattt gggacaggcc 20760 tggcttagca ggtccttaaa ctcgaccgac tataccgcga cgccgaggag ggggaggatt 20820 ggcgccgcat cgcgcggcga aacgcgggtg cgtcgcaaca tttcgccgga gtcgatccgt 20880 cgcgaatgct gcacccgcga acgcaatgac ggccgccacg caatccggct tgatcccggg 20940 cggcggatcg cgataagccg cgccacggtc gccaaaactc gtcgaaataa ccgacaaaac 21000 cacggcatat ggctggatat tgcagcgttt gccctgcgtt tccgtcgttc aaccgccctt 21060 cgaatcaggc aggcccagcg tgaccatgat tgatcttcct cttggaacgg cacactttgg 21120 tcgacacgga gacttccggt cgggcaattg tcccgttata gtgcaatgca acaggccgaa 21180 tcggccgctg tcggcgtgca cattccgttg agggagcccg atgaggcaat gaacgctttc 21240 gaagcacagc gcgcctttga ggagcaactt cgggcgcatt cccgggttac gccatctgcc 21300 gctcccgtgt ggcgtcgctc gacgctgcgg atggtcctct ataccgagtt gctgctgctg 21360 gacagtctct cgatcctggc cggattccac gtcgcggcgg gcacgcgcga cggcaactgg 21420 ctgtcgctgg cgggcatcaa cgtcggcgtc ttcctgctgc cgatcgctct cggcaccgcg 21480 ctcgcaagcg gcacctactc gctgaactgc ctgcgctacc cggtcagcgg cgtgaagagc 21540 atcttctcgg cattcttctt ctcgatcttc gtcgtcctgc tcggcagcta cctgctgacg 21600 gccgagctgc cgctgtcccg cgtgcagctg gcggagggcg cgatcctctc gctggtcctc 21660 ctgatggtgg gccgcctgat gttccgccgc cacgtccgcg cggttaccgg cggcaggctg 21720 ctcgacgaac tggtcatcat cgacggcgtc tcgctcgacg tcgcgggcaa tgcggtcgcg 21780 ctcgacgcgc ggatcatcaa tctctcgccg aacccgcgcg atccgcaaat gctgcatcgc 21840 ctgggcacca ccgtgatcgg gttcgaccgg gtgatcgtcg cctgcaccaa ggagcatcgc 21900 gcggtctggg cgctgctgct caagggcatg aacatcaagg gcgagatcct cgtcccccag 21960 ttcaatgcgc tgggcgcgat cggcgtggac gcctttgacg ggaaggatac gctggtcgtc 22020 tcgcagggcc cgctcaacat gcccaaccgc gcgaagaagc gcgcgctcga tctcgcgatc 22080 accgtaccgg ccgtgctcgc gctggcgccg ctgatgatcc tggtggcgat cctgatcaag 22140 ctggagagcc cgggcccggt gttgttcgcg caggatcgcg tcggccgcgg caaccggctg 22200 ttcaagatca tgaagttccg ctcgatgcgc gtaacgctgt gcgacgcgaa cggcaacgtc 22260 tcggccagcc gcgacgacga tcgcatcacc aaggtcggcc gcttcatccg caagaccagc 22320 atcgacgaac tgccgcagct gctgaacgtg ctgcgcggcg acatgagcgt cgtcggcccg 22380 cggccgcatg cgctgggctc gcgcgccgcc gatcacctgt tctgggaaat cgacgagcgc 22440 tactggcacc gccacacgct caagccgggc atgaccggtc tggcccaggt gcgcggtttc 22500 cgcggggcga ccgatcgccg cgtcgatctg accaaccggc tccaggcaga catggaatat 22560 atcgacggat gggatatctg gcgcgatatc acgatcctgt tcaagacgct gcgggtgatc 22620 gtgcattcga acgcattctg atccgcgcac gacgctgggc cgcagcctcg atccgcaaat 22680 ggattgacag cggcccggct tccgttttct cgtttgattt tcgttgcggc cggtccgcgc 22740 catgggggat tactgaatga agggcatcat ccttgcgggg ggcagcggga cgcgcctgta 22800 ccccgcaacg ctatcgatct cgaagcagct gcttcccgtc tatgacaagc cgatgatctt 22860 ctatccgctg tcggtgctga tgctcaccgg catccgggac atcctgatta tctccacccc 22920 gcgcgacctg ccgatgttcc aggcgctgct gggcgacggc tcggccttcg gcatcaacct 22980 cagctatgcc gagcagccct cccccaacgg gctggccgaa gcgttcatca tcggcgcgga 23040 tttcgtcggc aacgatccca gcgcgctgat cctgggcgac aacatctatc acggcgaaaa 23100 gatgggcgag cgctgccagg cagccgcagc gcaggcagcg cagggcggtg caaacgtctt 23160 cgcctatcat gtcgacgacc ccgagcgcta cggcgtggtc gcgttcgacc cggagacggg 23220 cgtcgccacc agcgtcgagg aaaagccggc cgagcccaag tccaactggg cgatcaccgg 23280 cctgtatttc tacgacaagg acgtggtcga catcgccaag tcgatccagc cctcggcgcg 23340 cggcgaactc gagatcaccg acgtcaaccg cgtttacatg gagcgcggcg acctgcacat 23400 cacgcgcctc ggccgcggct atgcctggct cgacaccggc acgcatgaca gcctgcacga 23460 agccggctcg ttcgttcgca cgctcgagca tcggacgggc gtgaagatcg cctgcccgga 23520 ggaaatcgcc ttcgaaagcg gctggctcgg cgccgaagac ctgctcaagc gcgccgccgg 23580 cctcggcaag accggctatg ccgcctatct ccgcaaggtt gcgaccgcag catgacccag 23640 gtccatcatc acgaactgtc cggcgtcatc gagttcacgc cgcccaaata tggcgaccac 23700 cgcggcttct tctccgaagt gttcaagcag tcggtgctcg atgccgaagg cgtcgaggca 23760 cgctgggtgc aggacaatca gagcttctcg gcggccccgg gcacgatccg cggcctgcat 23820 ctccaggcgc cgcccttcgc ccaggccaag ctggtccgcg tgttgcgcgg cgcgatcttc 23880 gacgtcgcgg tcgacatccg tcgcggctcg cccacctatg gcaaatgggt cggcgtcgag 23940 ctctcggccg agaagtggaa ccagctgctg gtccccgccg gctatgcgca cggcttcatg 24000 acgctcgttc cggattgcga gatcctctac aaggtcagcg ccaaatattc gaaggattcg 24060 gagatggcga tccgttggga cgatcccgat ctcgccatcg cctggccgga catcggcgtc 24120 gagccggtcc tctccgaaaa ggacgcggtc gccacgccct tcgccgaatt caacaccccc 24180 ttcttctatc agggctgagc catgcagcag accttcctcg tcaccggcgg cgccggcttc 24240 atcggctcgg cggtggtgcg ccacctcgtc cgccagggcg cgcgcgtcat caatctcgac 24300 aagctcacct atgccggcaa cccggcctcg ctgactgcga tcgagaacgc gcccaactat 24360 cgcttcgtcc atgccgacat cgccgacacc gcgacgatcc taccgctgct gcgcgaggag 24420 caggtcgatg tggtgatgca cctcgccgcc gagagccatg tcgatcgctc gatcgacggc 24480 cctggcgagt tcatcgagac caatgtcgtc ggcaccttca agctgctcca gtcggcgctg 24540 caatattggc gcgagctgga gggcgagaaa cgcgacgcgt tccgcttcca ccacatctcc 24600 accgacgaag tgttcggcga cctgccgttc gacagcggca tcttcaccga agagacgccc 24660 tatgatccct cctcgcccta ttcggcgtcg aaggcggcga gcgaccatct ggtgcgcgcc 24720 tggggccaca cctatggcct gccggtggtg ctgtcgaact gctcgaacaa ttacgggccg 24780 ttccacttcc ccgagaagct gatcccgttg accatcctca acgcgctcga gggcaagccg 24840 ctgccggtct acggcaaggg cgagaatatc cgcgactggc tgtatgtcga cgatcacgcc 24900 aaggcgctgg cgaccatcgc caccaccggc aaggtcggcc agagctacaa tgtcggcggc 24960 cgcaacgagc ggaccaacct gcaggtggtc gagacgatct gcgacctgct cgaccagcgc 25020 attccgctgg ccgacggtcg caagcgccgc gaactgatca ccttcgtcac cgatcgcccc 25080 ggccatgacc gccgctacgc gatcgacgcg accaagctcg agaccgagct gggctggaag 25140 gctgaggaga atttcgacac cggcatcgcc gcgacgatcg actggtatct ggcgaacgag 25200 tggtggtggg gcccgatccg ctccggcaaa tatgccggcg agcggctggg gcagaccgcc 25260 tgatgcgtat cctcgtcacc gggcatgacg gccaggtcgc ccagtcgctg gccgagcagg 25320 cggtgggcca cgagctggtc ttcaccacct accccgaatt cgatctctcc aagccggaga 25380 cgatcgaggc cggtgtggcg cgggtgcacc cggacctgat cgtctccgcc gccgcctaca 25440 cggcggtcga caaggcggaa agcgaacccg agctggcgat ggcgatcaac ggcgacggtc 25500 ccggcgtgct ggcgcgcgcg ggcgcgaaga tcggcgcgcc gatcatccac ctgtcgaccg 25560 attatgtgtt cgacggcagt ctcgaccgcc cttggcgcga ggacgatccc accggcccgc 25620 tcggcgtcta tggcgcgacc aagctggccg gcgagcaggc ggtgcaggcc tcgggtgcca 25680 ccaacgccgt gatccggctg gcctgggtct acagcccgtt cggcaacaat ttcgtcaaga 25740 cgatgctccg cctcgccgag acgcgcgacg cgctgaacgt cgtggaggac cagtggggct 25800 gccccagttc ggcgctggac atcgcgaccg cgatcctgac ggtggtcggg cactggcagc 25860 aggacggcgc gacgagcggc ctctaccatt tcgccggcac cggcgagacc aactgggccg 25920 acttcgcatc gacgatcttc gccgagagcg ccaagcgcgg tggcccctcg gccaccgtca 25980 ccggcattcc cagctcgggc tatccgactc cggccacgcg cccggccaat tcgcggctgg 26040 actgcacccg cttcgcggag accttcggct accgggcgcc tgcctggcag gattcgctga 26100 acgtcgtact ggatcgcctg ctcggctgat ccgaaacggg gggcctcagc gccccccgcc 26160 atgctcccgt tcgcgcgccg gcaatgcctc tagcaccgcg cgctttccct taggactcag 26220 ctcgctccag ccggcgattt ccttgggcga ccgccagcac cccaggcaca gccggatc 26278 <210> 2 <211> 1053 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (1053) <223> dpsS (partial) <400> 2 atc aac ggc gcc ttg ctc gga cgg cac aaa ttc gtc ctg gtc aat gtg 48 Ile Asn Gly Ala Leu Leu Gly Arg His Lys Phe Val Leu Val Asn Val  1 5 10 15   tcc acg gtc gcc tct tcg ata ctg ttc cag ctg ttc ccg ctt gtc gtc 96 Ser Thr Val Ala Ser Ser Ile Leu Phe Gln Leu Phe Pro Leu Val Val              20 25 30   gcc tgg atg atc ggc ccg gac ctg cga acg ctg ctg atc gcc gcg ctc 144 Ala Trp Met Ile Gly Pro Asp Leu Arg Thr Leu Leu Ile Ala Ala Leu          35 40 45   gtt ggc cgg gcg gtt ccg atg atc ggc atg ctg ccc gcg ctg tat cga 192 Val Gly Arg Ala Val Pro Met Ile Gly Met Leu Pro Ala Leu Tyr Arg      50 55 60   aac ctt ttg cgc ggc aac acg ccg cgt ttt cac gcc agc gag gcg cgc 240 Asn Leu Leu Arg Gly Asn Thr Pro Arg Phe His Ala Ser Glu Ala Arg  65 70 75 80   ttc ctg ata ggc tat ggc ggg tgg gcc tcg ctc acg acc gtg gta gcg 288 Phe Leu Ile Gly Tyr Gly Gly Trp Ala Ser Leu Thr Thr Val Val Ala                  85 90 95   acc gtg ctc atg atg gcg gac cgc ttc ctg att ggc gca ctt ctt ggg 336 Thr Val Leu Met Met Ala Asp Arg Phe Leu Ile Gly Ala Leu Leu Gly             100 105 110   ccc gtc gcc gtg acc atc tac acg gcc ccc ctg caa ctc gca cag cgc 384 Pro Val Ala Val Thr Ile Tyr Thr Ala Pro Leu Gln Leu Ala Gln Arg         115 120 125   gta tcg ctg ctg ccc tcc gca ctg tcc gcc gcg ctg ttc ccg cgc ctg 432 Val Ser Leu Leu Pro Ser Ala Leu Ser Ala Ala Leu Phe Pro Arg Leu     130 135 140   ccc agc gcg acg ccg gcg gag cgc atg gcg ctt cag atc cgc tcg ctg 480 Pro Ser Ala Thr Pro Ala Glu Arg Met Ala Leu Gln Ile Arg Ser Leu 145 150 155 160   tcg ctg atc atg ggc ggc ctt acc ggg atg atc ggc ggc gga cta ttg 528 Ser Leu Ile Met Gly Gly Leu Thr Gly Met Ile Gly Gly Gly Leu Leu                 165 170 175   ctg gcc gcg ccg ttt ctc gat ctc tgg atc ggc aag tcg ctc ggc cat 576 Leu Ala Ala Pro Phe Leu Asp Leu Trp Ile Gly Lys Ser Leu Gly His             180 185 190   gcg gga acg ccg gtc gcg ctc ttc ctg ttc ttc ggc gca tgg tgg aat 624 Ala Gly Thr Pro Val Ala Leu Phe Leu Phe Phe Gly Ala Trp Trp Asn         195 200 205   gcg ctg gcg atc att tcg ttc agc ggc ctg cag gcg agc gga cgg ccg 672 Ala Leu Ala Ile Ile Ser Phe Ser Gly Leu Gln Ala Ser Gly Arg Pro     210 215 220   aaa gcg agc gcg atc gtc cag ggg gca gag ctg cta ccc gtg ttg atc 720 Lys Ala Ser Ala Ile Val Gln Gly Ala Glu Leu Leu Pro Val Leu Ile 225 230 235 240   gcg ctg tat gca ggg atc cga tgg ggc ggc gtg acc ggc gcc gca gcg 768 Ala Leu Tyr Ala Gly Ile Arg Trp Gly Gly Val Thr Gly Ala Ala Ala                 245 250 255   gtc ttt ctg gga cgc tcc gcc ctg gat ttc gtc ctg ttg acc tgg cag 816 Val Phe Leu Gly Arg Ser Ala Leu Asp Phe Val Leu Leu Thr Trp Gln             260 265 270   gca ggc ctg ctc cgc cag acg gtg aag caa gta tcc gta tgc ggc gcc 864 Ala Gly Leu Leu Arg Gln Thr Val Lys Gln Val Ser Val Cys Gly Ala         275 280 285   gtt ctc acc gtc gcg atg ctc gtg ggc gcg acc tat cgc tat tcg gtg 912 Val Leu Thr Val Ala Met Leu Val Gly Ala Thr Tyr Arg Tyr Ser Val     290 295 300   ccg ctc tgg tgc gta ctc agc gcc tgc tgc ctg gtc gcg ctg gca gcc 960 Pro Leu Trp Cys Val Leu Ser Ala Cys Cys Leu Val Ala Leu Ala Ala 305 310 315 320   tgc tcc tgg tgg aca ttg gcg cgc cag gac aag gca ctg ctg att gga 1008 Cys Ser Trp Trp Thr Leu Ala Arg Gln Asp Lys Ala Leu Leu Ile Gly                 325 330 335   cga ttg agc cga att cta cca aag cag cgg caa ctc gac cta tag 1053 Arg Leu Ser Arg Ile Leu Pro Lys Gln Arg Gln Leu Asp Leu *             340 345 350   <210> 3 <211> 350 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> homologous to gel S <400> 3 Ile Asn Gly Ala Leu Leu Gly Arg His Lys Phe Val Leu Val Asn Val  1 5 10 15 Ser Thr Val Ala Ser Ser Ile Leu Phe Gln Leu Phe Pro Leu Val Val             20 25 30 Ala Trp Met Ile Gly Pro Asp Leu Arg Thr Leu Leu Ile Ala Ala Leu         35 40 45 Val Gly Arg Ala Val Pro Met Ile Gly Met Leu Pro Ala Leu Tyr Arg     50 55 60 Asn Leu Leu Arg Gly Asn Thr Pro Arg Phe His Ala Ser Glu Ala Arg 65 70 75 80 Phe Leu Ile Gly Tyr Gly Gly Trp Ala Ser Leu Thr Thr Val Val Ala                 85 90 95 Thr Val Leu Met Met Ala Asp Arg Phe Leu Ile Gly Ala Leu Leu Gly             100 105 110 Pro Val Ala Val Thr Ile Tyr Thr Ala Pro Leu Gln Leu Ala Gln Arg         115 120 125 Val Ser Leu Leu Pro Ser Ala Leu Ser Ala Ala Leu Phe Pro Arg Leu     130 135 140 Pro Ser Ala Thr Pro Ala Glu Arg Met Ala Leu Gln Ile Arg Ser Leu 145 150 155 160 Ser Leu Ile Met Gly Gly Leu Thr Gly Met Ile Gly Gly Gly Leu Leu                 165 170 175 Leu Ala Ala Pro Phe Leu Asp Leu Trp Ile Gly Lys Ser Leu Gly His             180 185 190 Ala Gly Thr Pro Val Ala Leu Phe Leu Phe Phe Gly Ala Trp Trp Asn         195 200 205 Ala Leu Ala Ile Ile Ser Phe Ser Gly Leu Gln Ala Ser Gly Arg Pro     210 215 220 Lys Ala Ser Ala Ile Val Gln Gly Ala Glu Leu Leu Pro Val Leu Ile 225 230 235 240 Ala Leu Tyr Ala Gly Ile Arg Trp Gly Gly Val Thr Gly Ala Ala Ala                 245 250 255 Val Phe Leu Gly Arg Ser Ala Leu Asp Phe Val Leu Leu Thr Trp Gln             260 265 270 Ala Gly Leu Leu Arg Gln Thr Val Lys Gln Val Ser Val Cys Gly Ala         275 280 285 Val Leu Thr Val Ala Met Leu Val Gly Ala Thr Tyr Arg Tyr Ser Val     290 295 300 Pro Leu Trp Cys Val Leu Ser Ala Cys Cys Leu Val Ala Leu Ala Ala 305 310 315 320 Cys Ser Trp Trp Thr Leu Ala Arg Gln Asp Lys Ala Leu Leu Ile Gly                 325 330 335 Arg Leu Ser Arg Ile Leu Pro Lys Gln Arg Gln Leu Asp Leu             340 345 350 <210> 4 <211> 1626 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (1626) <223> dpsG <400> 4 atg acg act acc tcg gcg ttt cgt cgc ccg gcc ttc cac gga gcg atg 48 Met Thr Thr Thr Ser Ser Ala Phe Arg Arg Pro Ala Phe His Gly Ala Met  1 5 10 15   cag cgg ctt cgc agg ttg cga ctg gtt cgg ttt ctg aca aag cca gcg 96 Gln Arg Leu Arg Arg Leu Arg Leu Val Arg Phe Leu Thr Lys Pro Ala              20 25 30   atc ccg gta ctg ccc gtc tac aaa gcc gag cga tca ggc gtg acg atc 144 Ile Pro Val Leu Pro Val Tyr Lys Ala Glu Arg Ser Gly Val Thr Ile          35 40 45   gcg gcg cgg cgt acc gtt ctg ctg gtc agc gtg atg ttt ctt gcc gca 192 Ala Ala Arg Arg Thr Val Leu Leu Val Ser Val Met Phe Leu Ala Ala      50 55 60   gtc tac ggc ctg ctc gcc gca gtt ctg ccg ctc cag atg ctg gcg atc 240 Val Tyr Gly Leu Leu Ala Ala Val Leu Pro Leu Gln Met Leu Ala Ile  65 70 75 80   ccg gcc gtg ccc ctc gtt ctg atg gcg ctc gta gtg atc tgg gcg cta 288 Pro Ala Val Pro Leu Val Leu Met Ala Leu Val Val Ile Trp Ala Leu                  85 90 95   ccc gag gcg cgg cag gcg cct act cgc ctg ctg gca aaa cta tac ctc 336 Pro Glu Ala Arg Gln Ala Pro Thr Arg Leu Leu Ala Lys Leu Tyr Leu             100 105 110   gcc tat atg gtg gcg gcg ctc gtg tgg ccg aac tat ctt gcg ctc agc 384 Ala Tyr Met Val Ala Ala Leu Val Trp Pro Asn Tyr Leu Ala Leu Ser         115 120 125   gtt ggt ggt ttg cct tgg atc tcg atc agg cgg atg atc ggt tcg atc 432 Val Gly Gly Leu Pro Trp Ile Ser Ile Arg Arg Met Ile Gly Ser Ile     130 135 140   gca ttg ctc acg ctc atg atc tcg ctt tcg gtc tcg aaa aag ttt cga 480 Ala Leu Leu Thr Leu Met Ile Ser Leu Ser Val Ser Lys Lys Phe Arg 145 150 155 160   tcc gag atg gcg gcg att atg cgg gcg gcg ccg atc cca tcg cga cta 528 Ser Glu Met Ala Ala Ile Met Arg Ala Ala Pro Ile Pro Ser Arg Leu                 165 170 175   ttg ctt gcc ttc att atg gtc cag atc gtc gcc tcc att gcg acc cca 576 Leu Leu Ala Phe Ile Met Val Gln Ile Val Ala Ser Ile Ala Thr Pro             180 185 190   gcg gcc tcg caa aca att ccg cgg ttg att ggc atc gtt ctg acg gtg 624 Ala Ala Ser Gln Thr Ile Pro Arg Leu Ile Gly Ile Val Leu Thr Val         195 200 205   acg ccg atg gca ttc atc tcg cta tgg ctg atc ggg acc gac acg cgc 672 Thr Pro Met Ala Phe Ile Ser Leu Trp Leu Ile Gly Thr Asp Thr Arg     210 215 220   acc ccg gag tgg tgg gtt acg cgc ttg ttt tgg tgc gtg ggc gtg ctg 720 Thr Pro Glu Trp Trp Val Thr Arg Leu Phe Trp Cys Val Gly Val Leu 225 230 235 240   atg gcg att ggc gtg ctg gag ttc cgg gtg aag cac gtc ctc tgg gcc 768 Met Ala Ile Gly Val Leu Glu Phe Arg Val Lys His Val Leu Trp Ala                 245 250 255   tac tcg att ccg agc ttc ctg cgc gtt gat gag cag ttt ctt acc gta 816 Tyr Ser Ile Pro Ser Phe Leu Arg Val Asp Glu Gln Phe Leu Thr Val             260 265 270   gtg ctt acg ccc ggt ttc cgg ggt act tac cgc gtt ttg acg act ttc 864 Val Leu Thr Pro Gly Phe Arg Gly Thr Tyr Arg Val Leu Thr Thr Phe         275 280 285   agc agt ccg ctt gtc tgg gga gag ttg acg gct ttg acg att cct ttc 912 Ser Ser Pro Leu Val Trp Gly Glu Leu Thr Ala Leu Thr Ile Pro Phe     290 295 300   gtg ctg cac cgt ata gcg aat tcc cgt ggc gta ggg aga ttg gca ttt 960 Val Leu His Arg Ile Ala Asn Ser Arg Gly Val Gly Arg Leu Ala Phe 305 310 315 320   tgg att ttc ttc gat ttt ctt gta gtt gcg tcc gga ttt ctt agc ggt 1008 Trp Ile Phe Phe Asp Phe Leu Val Val Ala Ser Gly Phe Leu Ser Gly                 325 330 335   tct cga ctg gca atg gtg ggg gga ttg gtg gcg cat acc gtg tat ttg 1056 Ser Arg Leu Ala Met Val Gly Gly Leu Val Ala His Thr Val Tyr Leu             340 345 350   ctt att tgg gca att cgg cgg tgg cgg acc aca aag ggc ggg ctt gtc 1104 Leu Ile Trp Ala Ile Arg Arg Trp Arg Thr Thr Lys Gly Gly Leu Val         355 360 365   ggc att tcg ctg acg ttg acg tat ccc gcc ttg atg gtg gcg ctg tcg 1152 Gly Ile Ser Leu Thr Leu Thr Tyr Pro Ala Leu Met Val Ala Leu Ser     370 375 380   ctg gcg gtc atg ttc gtg cct gcc gtc cac aac cgt gtt ctc ggt gga 1200 Leu Ala Val Met Phe Val Pro Ala Val His Asn Arg Val Leu Gly Gly 385 390 395 400   ggc gct tct caa ctt agc aat caa ggc agg cag gag cag ttt cgc ttg 1248 Gly Ala Ser Gln Leu Ser Asn Gln Gly Arg Gln Glu Gln Phe Arg Leu                 405 410 415   ggc gtg cct gcc atc gct cgc cgc cct ttc ttc ggc tat ggc ccg gga 1296 Gly Val Pro Ala Ile Ala Arg Arg Pro Phe Phe Gly Tyr Gly Pro Gly             420 425 430   gag ggt gct ggc gct gtc ggg tgg cgt aat cag caa ggc ttc cta tcg 1344 Glu Gly Ala Gly Ala Val Gly Trp Arg Asn Gln Gln Gly Phe Leu Ser         435 440 445   atc gac agt ggt ttc ctg tcc gta gcg gct gat tac ggc ttg ctg ggt 1392 Ile Asp Ser Gly Phe Leu Ser Val Ala Ala Asp Tyr Gly Leu Leu Gly     450 455 460   ttt gtt tcg cta tac ggc acg atg att acg tta atg att ttg ctc gcg 1440 Phe Val Ser Leu Tyr Gly Thr Met Ile Thr Leu Met Ile Leu Leu Ala 465 470 475 480   ttt cgt ggt ctt aag atg agc gga gat ggc tac ccg ctt gag ctc gcc 1488 Phe Arg Gly Leu Lys Met Ser Gly Asp Gly Tyr Pro Leu Glu Leu Ala                 485 490 495   gta gca act ttc ctg gct gtt ctt tta aat acg cgg tca gtc ctg tcg 1536 Val Ala Thr Phe Leu Ala Val Leu Leu Asn Thr Arg Ser Val Leu Ser             500 505 510   caa ggt gac aat gac ccg ttc atc ttt atg acg ctg ggg cta ggc ata 1584 Gln Gly Asp Asn Asp Pro Phe Ile Phe Met Thr Leu Gly Leu Gly Ile         515 520 525   gcg ctt cta tat cgc tct cgg cct gtg tct ttg tcg gtg tag 1626 Ala Leu Leu Tyr Arg Ser Arg Pro Val Ser Leu Ser Val *     530 535 540   <210> 5 <211> 541 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative polymerase <400> 5 Met Thr Thr Thr Ser Ser Ala Phe Arg Arg Pro Ala Phe His Gly Ala Met  1 5 10 15 Gln Arg Leu Arg Arg Leu Arg Leu Val Arg Phe Leu Thr Lys Pro Ala             20 25 30 Ile Pro Val Leu Pro Val Tyr Lys Ala Glu Arg Ser Gly Val Thr Ile         35 40 45 Ala Ala Arg Arg Thr Val Leu Leu Val Ser Val Met Phe Leu Ala Ala     50 55 60 Val Tyr Gly Leu Leu Ala Ala Val Leu Pro Leu Gln Met Leu Ala Ile 65 70 75 80 Pro Ala Val Pro Leu Val Leu Met Ala Leu Val Val Ile Trp Ala Leu                 85 90 95 Pro Glu Ala Arg Gln Ala Pro Thr Arg Leu Leu Ala Lys Leu Tyr Leu             100 105 110 Ala Tyr Met Val Ala Ala Leu Val Trp Pro Asn Tyr Leu Ala Leu Ser         115 120 125 Val Gly Gly Leu Pro Trp Ile Ser Ile Arg Arg Met Ile Gly Ser Ile     130 135 140 Ala Leu Leu Thr Leu Met Ile Ser Leu Ser Val Ser Lys Lys Phe Arg 145 150 155 160 Ser Glu Met Ala Ala Ile Met Arg Ala Ala Pro Ile Pro Ser Arg Leu                 165 170 175 Leu Leu Ala Phe Ile Met Val Gln Ile Val Ala Ser Ile Ala Thr Pro             180 185 190 Ala Ala Ser Gln Thr Ile Pro Arg Leu Ile Gly Ile Val Leu Thr Val         195 200 205 Thr Pro Met Ala Phe Ile Ser Leu Trp Leu Ile Gly Thr Asp Thr Arg     210 215 220 Thr Pro Glu Trp Trp Val Thr Arg Leu Phe Trp Cys Val Gly Val Leu 225 230 235 240 Met Ala Ile Gly Val Leu Glu Phe Arg Val Lys His Val Leu Trp Ala                 245 250 255 Tyr Ser Ile Pro Ser Phe Leu Arg Val Asp Glu Gln Phe Leu Thr Val             260 265 270 Val Leu Thr Pro Gly Phe Arg Gly Thr Tyr Arg Val Leu Thr Thr Phe         275 280 285 Ser Ser Pro Leu Val Trp Gly Glu Leu Thr Ala Leu Thr Ile Pro Phe     290 295 300 Val Leu His Arg Ile Ala Asn Ser Arg Gly Val Gly Arg Leu Ala Phe 305 310 315 320 Trp Ile Phe Phe Asp Phe Leu Val Val Ala Ser Gly Phe Leu Ser Gly                 325 330 335 Ser Arg Leu Ala Met Val Gly Gly Leu Val Ala His Thr Val Tyr Leu             340 345 350 Leu Ile Trp Ala Ile Arg Arg Trp Arg Thr Thr Lys Gly Gly Leu Val         355 360 365 Gly Ile Ser Leu Thr Leu Thr Tyr Pro Ala Leu Met Val Ala Leu Ser     370 375 380 Leu Ala Val Met Phe Val Pro Ala Val His Asn Arg Val Leu Gly Gly 385 390 395 400 Gly Ala Ser Gln Leu Ser Asn Gln Gly Arg Gln Glu Gln Phe Arg Leu                 405 410 415 Gly Val Pro Ala Ile Ala Arg Arg Pro Phe Phe Gly Tyr Gly Pro Gly             420 425 430 Glu Gly Ala Gly Ala Val Gly Trp Arg Asn Gln Gln Gly Phe Leu Ser         435 440 445 Ile Asp Ser Gly Phe Leu Ser Val Ala Ala Asp Tyr Gly Leu Leu Gly     450 455 460 Phe Val Ser Leu Tyr Gly Thr Met Ile Thr Leu Met Ile Leu Leu Ala 465 470 475 480 Phe Arg Gly Leu Lys Met Ser Gly Asp Gly Tyr Pro Leu Glu Leu Ala                 485 490 495 Val Ala Thr Phe Leu Ala Val Leu Leu Asn Thr Arg Ser Val Leu Ser             500 505 510 Gln Gly Asp Asn Asp Pro Phe Ile Phe Met Thr Leu Gly Leu Gly Ile         515 520 525 Ala Leu Leu Tyr Arg Ser Arg Pro Val Ser Leu Ser Val     530 535 540 <210> 6 <211> 1998 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) (1998) <223> dpsR <400> 6 atg ccg gat atc att gtc aag aat cag acg gag ttg aat gct gca atc 48 Met Pro Asp Ile Ile Val Lys Asn Gln Thr Glu Leu Asn Ala Ala Ile  1 5 10 15   gcg gcg gcg aag ggt ggc gaa acc atc aag ctt gcc gcc ggg gtc tac 96 Ala Ala Ala Lys Gly Gly Glu Thr Ile Lys Leu Ala Ala Gly Val Tyr              20 25 30   aca gat ctc act gta atg acc aag acg ttt acc agc atg gtg aca att 144 Thr Asp Leu Thr Val Met Thr Lys Thr Phe Thr Ser Met Val Thr Ile          35 40 45   gag tcg ctc gac tcg tcg aac ccg gtc aat atc caa aag ctg gtg atc 192 Glu Ser Leu Asp Ser Ser Asn Pro Val Asn Ile Gln Lys Leu Val Ile      50 55 60   ggg aac agt agc aac gtt acc gtc aaa aac atg gtc gct gcg acc gat 240 Gly Asn Ser Ser Asn Val Thr Val Lys Asn Met Val Ala Ala Thr Asp  65 70 75 80   tac aag ccc gcc gat gac tgg aat cga ctg aat acg atc cag ggt tcg 288 Tyr Lys Pro Ala Asp Asp Trp Asn Arg Leu Asn Thr Ile Gln Gly Ser                  85 90 95   gcc aac atc gtt ttg gac ggc gtg cgg ttc agc ggc ggc act ggt gac 336 Ala Asn Ile Val Leu Asp Gly Val Arg Phe Ser Gly Gly Thr Gly Asp             100 105 110   cct tcg ctc tcg aag ggg gcg ggc ttg ttc gtg cgc aac agc acg tcg 384 Pro Ser Leu Ser Lys Gly Ala Gly Leu Phe Val Arg Asn Ser Thr Ser         115 120 125   gtg acg atg cag aat tcg tct atc gac cac ttc ggt ctg ggc ctt gag 432 Val Thr Met Gln Asn Ser Ser Ile Asp His Phe Gly Leu Gly Leu Glu     130 135 140   gcc tac aac gtc gat ggc atg gtg gtc cag aac agc agc ttc cac gac 480 Ala Tyr Asn Val Asp Gly Met Val Val Gln Asn Ser Ser Phe His Asp 145 150 155 160   aac cgg cgc gat cat acg aac ttc act gag atg aac aat ctt gtc atc 528 Asn Arg Arg Asp His Thr Asn Phe Thr Glu Met Asn Asn Leu Val Ile                 165 170 175   gac gga aat tcg ttc acg aac ctg ttt ccc gtg ggc acc gaa cat ccc 576 Asp Gly Asn Ser Phe Thr Asn Leu Phe Pro Val Gly Thr Glu His Pro             180 185 190   gac gcc att cag ttc ttc acg gcg ggc aag gtc aag ggc aat acc aac 624 Asp Ala Ile Gln Phe Phe Thr Ala Gly Lys Val Lys Gly Asn Thr Asn         195 200 205   atc acc atc tcc aat aac gtc atc atg cag ggc gcg ggc tct ggc gcg 672 Ile Thr Ile Ser Asn Asn Val Ile Met Gln Gly Ala Gly Ser Gly Ala     210 215 220   caa ggg att ttc atg aat gac gag gcc ggt aat ctt ccc tat gtc aat 720 Gln Gly Ile Phe Met Asn Asp Glu Ala Gly Asn Leu Pro Tyr Val Asn 225 230 235 240   gta aac atc aaa aac aat ctt atc tat ctg aat ggt tat tac cac ggt 768 Val Asn Ile Lys Asn Asn Leu Ile Tyr Leu Asn Gly Tyr Tyr His Gly                 245 250 255   atc aac gtt gtt aac ggc gtt aat gtc aat atc gaa tcc aat agc gtg 816 Ile Asn Val Val Asn Gly Val Asn Val Asn Ile Glu Ser Asn Ser Val             260 265 270   ata tcg caa gtg gat ggc aca tca ttt tgg att cgc ctc gac aaa acc 864 Ile Ser Gln Val Asp Gly Thr Ser Phe Trp Ile Arg Leu Asp Lys Thr         275 280 285   aat ggc gcg acg atc aag aac aat gtt gcg gac ctg atc acc gtc aca 912 Asn Gly Ala Thr Ile Lys Asn Asn Val Ala Asp Leu Ile Thr Val Thr     290 295 300   agc tcc tcg agc aat atc gtg cag aca ggc aat cgt acg ctg acg agt 960 Ser Ser Ser Ser Asn Ile Val Gln Thr Gly Asn Arg Thr Leu Thr Ser 305 310 315 320   gac tcg gca acg atc cgc aag atc tat ggc ctc aac gat ggg gct acg 1008 Asp Ser Ala Thr Ile Arg Lys Ile Tyr Gly Leu Asn Asp Gly Ala Thr                 325 330 335   gcg cgg ctc agc gat ttg atc gtt ccc ggc gtc ggg tac cag ccg ccc 1056 Ala Arg Leu Ser Asp Leu Ile Val Pro Gly Val Gly Tyr Gln Pro Pro             340 345 350   gtg tcg agc gct gct gcc gct cag gtg act acc gaa ctg tcg act gcg 1104 Val Ser Ser Ala Ala Ala Ala Gln Val Thr Thr Glu Leu Ser Thr Ala         355 360 365   aag gcg gca aat ccg tcg ctg ctg ctc gat ctg tcg ttc agc aac agc 1152 Lys Ala Ala Asn Pro Ser Leu Leu Leu Asp Leu Ser Phe Ser Asn Ser     370 375 380   ggc gtc gtg gac ctt tcg cac tgg aat acc ggc cag acg aca aag gcg 1200 Gly Val Val Asp Leu Ser His Trp Asn Thr Gly Gln Thr Thr Lys Ala 385 390 395 400   gtg gac gtg tcg gcg gtc gtg ggc agc gcc ttc cac gtc tcg acg ggc 1248 Val Asp Val Ser Ala Val Val Gly Ser Ala Phe His Val Ser Thr Gly                 405 410 415   acg ggg gtg gaa cta aac cgg agc tat tcg cgg cag att tac gca ttg 1296 Thr Gly Val Glu Leu Asn Arg Ser Tyr Ser Arg Gln Ile Tyr Ala Leu             420 425 430   tcg gcg ttc acg ctc agc ttc gac ctc aag cgg gac tcg gct acg gcc 1344 Ser Ala Phe Thr Leu Ser Phe Asp Leu Lys Arg Asp Ser Ala Thr Ala         435 440 445   acg gcc ggg caa att ctt ggc atc ttc cag agc tgg tcg gtt tcg ctg 1392 Thr Ala Gly Gln Ile Leu Gly Ile Phe Gln Ser Trp Ser Val Ser Leu     450 455 460   cag gcc aat ggg gaa ctg agc ttc acc atg cgc aac gcc gcg ggc gtc 1440 Gln Ala Asn Gly Glu Leu Ser Phe Thr Met Arg Asn Ala Ala Gly Val 465 470 475 480   agc cag aca atg gtg acg agc ggc gcc aag ctg ctt gat gct gcc aca 1488 Ser Gln Thr Met Val Thr Ser Gly Ala Lys Leu Leu Asp Ala Ala Thr                 485 490 495   cac aag atc gcc ctg acc tac gac agc acg cgg aaa acc gcg att ctg 1536 His Lys Ile Ala Leu Thr Tyr Asp Ser Thr Arg Lys Thr Ala Ile Leu             500 505 510   tac gta gac ggc atg caa cgc ggc aca gcg acg atg acc ggc acg acc 1584 Tyr Val Asp Gly Met Gln Arg Gly Thr Ala Thr Met Thr Gly Thr Thr         515 520 525   cgg ccc gcc gaa tcc tgg ggg ctg tat gtc ggc agc ccg ttc tcg acc 1632 Arg Pro Ala Glu Ser Trp Gly Leu Tyr Val Gly Ser Pro Phe Ser Thr     530 535 540   gca ttc agc gga acg gtc ggc gac atc gag atc cgc gat ggc gcg atc 1680 Ala Phe Ser Gly Thr Val Gly Asp Ile Glu Ile Arg Asp Gly Ala Ile 545 550 555 560   agc gcc gcc cag gtg cag gcg ctg gtg acc gcg tcg agc gcc agc gcg 1728 Ser Ala Ala Gln Val Gln Ala Leu Val Thr Ala Ser Ser Ala Ser Ala                 565 570 575   gcg gcg acg gtc aag gac agc ctc gtc acc ggc gcg gcc gcg cag gcc 1776 Ala Ala Thr Val Lys Asp Ser Leu Val Thr Gly Ala Ala Ala Gln Ala             580 585 590   gct gcg ctg ctg gcg ggt gcc ggc gcc gct agc acg gca acg ccg ctt 1824 Ala Ala Leu Leu Ala Gly Ala Gly Ala Ala Ser Thr Ala Thr Pro Leu         595 600 605   gcg acg gtg gcc acg gtg ggc agt acg ctg tct ata ggt act gcc gcg 1872 Ala Thr Val Ala Thr Val Gly Ser Thr Leu Ser Ile Gly Thr Ala Ala     610 615 620   tcc tcg cag atc gcg ctc gtc agc aag atc ggt gtc gac atg atg acc 1920 Ser Ser Gln Ile Ala Leu Val Ser Lys Ile Gly Val Asp Met Met Thr 625 630 635 640   gcg ggg gcg atg ggc gca atc cgc agc gcg gcg aca ctg agc gct acg 1968 Ala Gly Ala Met Gly Ala Ile Arg Ser Ala Ala Thr Leu Ser Ala Thr                 645 650 655   gcg gat cag tac aac ctg tac cgc gcc tga 1998 Ala Asp Gln Tyr Asn Leu Tyr Arg Ala *             660 665   <210> 7 <211> 665 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative lyase <400> 7 Met Pro Asp Ile Ile Val Lys Asn Gln Thr Glu Leu Asn Ala Ala Ile  1 5 10 15 Ala Ala Ala Lys Gly Gly Glu Thr Ile Lys Leu Ala Ala Gly Val Tyr             20 25 30 Thr Asp Leu Thr Val Met Thr Lys Thr Phe Thr Ser Met Val Thr Ile         35 40 45 Glu Ser Leu Asp Ser Ser Asn Pro Val Asn Ile Gln Lys Leu Val Ile     50 55 60 Gly Asn Ser Ser Asn Val Thr Val Lys Asn Met Val Ala Ala Thr Asp 65 70 75 80 Tyr Lys Pro Ala Asp Asp Trp Asn Arg Leu Asn Thr Ile Gln Gly Ser                 85 90 95 Ala Asn Ile Val Leu Asp Gly Val Arg Phe Ser Gly Gly Thr Gly Asp             100 105 110 Pro Ser Leu Ser Lys Gly Ala Gly Leu Phe Val Arg Asn Ser Thr Ser         115 120 125 Val Thr Met Gln Asn Ser Ser Ile Asp His Phe Gly Leu Gly Leu Glu     130 135 140 Ala Tyr Asn Val Asp Gly Met Val Val Gln Asn Ser Ser Phe His Asp 145 150 155 160 Asn Arg Arg Asp His Thr Asn Phe Thr Glu Met Asn Asn Leu Val Ile                 165 170 175 Asp Gly Asn Ser Phe Thr Asn Leu Phe Pro Val Gly Thr Glu His Pro             180 185 190 Asp Ala Ile Gln Phe Phe Thr Ala Gly Lys Val Lys Gly Asn Thr Asn         195 200 205 Ile Thr Ile Ser Asn Asn Val Ile Met Gln Gly Ala Gly Ser Gly Ala     210 215 220 Gln Gly Ile Phe Met Asn Asp Glu Ala Gly Asn Leu Pro Tyr Val Asn 225 230 235 240 Val Asn Ile Lys Asn Asn Leu Ile Tyr Leu Asn Gly Tyr Tyr His Gly                 245 250 255 Ile Asn Val Val Asn Gly Val Asn Val Asn Ile Glu Ser Asn Ser Val             260 265 270 Ile Ser Gln Val Asp Gly Thr Ser Phe Trp Ile Arg Leu Asp Lys Thr         275 280 285 Asn Gly Ala Thr Ile Lys Asn Asn Val Ala Asp Leu Ile Thr Val Thr     290 295 300 Ser Ser Ser Ser Asn Ile Val Gln Thr Gly Asn Arg Thr Leu Thr Ser 305 310 315 320 Asp Ser Ala Thr Ile Arg Lys Ile Tyr Gly Leu Asn Asp Gly Ala Thr                 325 330 335 Ala Arg Leu Ser Asp Leu Ile Val Pro Gly Val Gly Tyr Gln Pro Pro             340 345 350 Val Ser Ser Ala Ala Ala Ala Gln Val Thr Thr Glu Leu Ser Thr Ala         355 360 365 Lys Ala Ala Asn Pro Ser Leu Leu Leu Asp Leu Ser Phe Ser Asn Ser     370 375 380 Gly Val Val Asp Leu Ser His Trp Asn Thr Gly Gln Thr Thr Lys Ala 385 390 395 400 Val Asp Val Ser Ala Val Val Gly Ser Ala Phe His Val Ser Thr Gly                 405 410 415 Thr Gly Val Glu Leu Asn Arg Ser Tyr Ser Arg Gln Ile Tyr Ala Leu             420 425 430 Ser Ala Phe Thr Leu Ser Phe Asp Leu Lys Arg Asp Ser Ala Thr Ala         435 440 445 Thr Ala Gly Gln Ile Leu Gly Ile Phe Gln Ser Trp Ser Val Ser Leu     450 455 460 Gln Ala Asn Gly Glu Leu Ser Phe Thr Met Arg Asn Ala Ala Gly Val 465 470 475 480 Ser Gln Thr Met Val Thr Ser Gly Ala Lys Leu Leu Asp Ala Ala Thr                 485 490 495 His Lys Ile Ala Leu Thr Tyr Asp Ser Thr Arg Lys Thr Ala Ile Leu             500 505 510 Tyr Val Asp Gly Met Gln Arg Gly Thr Ala Thr Met Thr Gly Thr Thr         515 520 525 Arg Pro Ala Glu Ser Trp Gly Leu Tyr Val Gly Ser Pro Phe Ser Thr     530 535 540 Ala Phe Ser Gly Thr Val Gly Asp Ile Glu Ile Arg Asp Gly Ala Ile 545 550 555 560 Ser Ala Ala Gln Val Gln Ala Leu Val Thr Ala Ser Ser Ala Ser Ala                 565 570 575 Ala Ala Thr Val Lys Asp Ser Leu Val Thr Gly Ala Ala Ala Gln Ala             580 585 590 Ala Ala Leu Leu Ala Gly Ala Gly Ala Ala Ser Thr Ala Thr Pro Leu         595 600 605 Ala Thr Val Ala Thr Val Gly Ser Thr Leu Ser Ile Gly Thr Ala Ala     610 615 620 Ser Ser Gln Ile Ala Leu Val Ser Lys Ile Gly Val Asp Met Met Thr 625 630 635 640 Ala Gly Ala Met Gly Ala Ile Arg Ser Ala Ala Thr Leu Ser Ala Thr                 645 650 655 Ala Asp Gln Tyr Asn Leu Tyr Arg Ala             660 665 <210> 8 <211> 939 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS <222> (1) ... (939) <223> dpsQ <400> 8 atg cag atg ctc cca acg ccc gat gtc agc ata ctc gtg gtc gct ttc 48 Met Gln Met Leu Pro Thr Pro Asp Val Ser Ile Leu Val Val Ala Phe  1 5 10 15   aac tcg acc gag tat atc gaa gac tgc ctg cgc ggc atc gcc gaa gga 96 Asn Ser Thr Glu Tyr Ile Glu Asp Cys Leu Arg Gly Ile Ala Glu Gly              20 25 30   gcg ggc aag acc ccc cac gaa gtt ctg ctg atc gac aat ggc gac ggg 144 Ala Gly Lys Thr Pro His Glu Val Leu Leu Ile Asp Asn Gly Asp Gly          35 40 45   cga acc gaa gcg ctg gtc cgg cag cgg ttc cac cac gtc cgc atc gtt 192 Arg Thr Glu Ala Leu Val Arg Gln Arg Phe His His Val Arg Ile Val      50 55 60   ccc agt gag ggc aat att ggt ttc ggg gcc ggc aat aat cgc ctg gca 240 Pro Ser Glu Gly Asn Ile Gly Phe Gly Ala Gly Asn Asn Arg Leu Ala  65 70 75 80   gcg cag gct gcc ggc ccg ctc ctg ctg ctc gtc aac ccc gat gcc att 288 Ala Gln Ala Ala Gly Pro Leu Leu Leu Leu Val Asn Pro Asp Ala Ile                  85 90 95   ccc cag ccc ggc gca atc gat cag ttg gtc acc ttt gcc aaa cag cat 336 Pro Gln Pro Gly Ala Ile Asp Gln Leu Val Thr Phe Ala Lys Gln His             100 105 110   ccc gag gcg gcg gca tgg ggc ggc cgt tcc tac tcg ccc agc ggc gat 384 Pro Glu Ala Ala Ala Trp Gly Gly Arg Ser Tyr Ser Pro Ser Gly Asp         115 120 125   cta gaa ccc gca aat ttc atg tcc ctg ccg acg ccc gcc gac ttt ctg 432 Leu Glu Pro Ala Asn Phe Met Ser Leu Pro Thr Pro Ala Asp Phe Leu     130 135 140   acg gcg att ttc aac gcg cgt gcg cta cgc agc ggc ggg ctg caa gaa 480 Thr Ala Ile Phe Asn Ala Arg Ala Leu Arg Ser Gly Gly Leu Gln Glu 145 150 155 160   ggc gcg acc acc ccc gga gcg gtc gag gtg ttg aat ggc ggc ttc atg 528 Gly Ala Thr Thr Pro Gly Ala Val Glu Val Leu Asn Gly Gly Phe Met                 165 170 175   atg gta cgc acc gat gtc tgg cag gcg atc ggc ggt ttt gac gag agc 576 Met Val Arg Thr Asp Val Trp Gln Ala Ile Gly Gly Phe Asp Glu Ser             180 185 190   ttt ttt ctt tat tcg gaa gag atc gat ctc ttc cag cga atc cgc acg 624 Phe Phe Leu Tyr Ser Glu Glu Ile Asp Leu Phe Gln Arg Ile Arg Thr         195 200 205   ttg ggg cac aag gtg ctc gtc gac ccc tcg gtc aaa gtg gta cac aat 672 Leu Gly His Lys Val Leu Val Asp Pro Ser Val Lys Val Val His Asn     210 215 220   acg ggg agt ggt cag tcg atg tcc cag aac cgc ctg atg tat ctc acg 720 Thr Gly Ser Gly Gln Ser Met Ser Gln Asn Arg Leu Met Tyr Leu Thr 225 230 235 240   acc ggg cgc atg cac tat gcg cga aag cat ttt ggc gca ctc ggc acc 768 Thr Gly Arg Met His Tyr Ala Arg Lys His Phe Gly Ala Leu Gly Thr                 245 250 255   ctt gcc acc ggg tgc gcg ctt tgg ctg atc gcc gcc aaa tac acg ttg 816 Leu Ala Thr Gly Cys Ala Leu Trp Leu Ile Ala Ala Lys Tyr Thr Leu             260 265 270   gtc ggg gcg gca ctc tgg cgc ctg tcg ccg cgg acg ggc acg cga tac 864 Val Gly Ala Ala Leu Trp Arg Leu Ser Pro Arg Thr Gly Thr Arg Tyr         275 280 285   aaa gag ctg agc aac ggg tgg cgt gcc gta ttt agc aat cct ggc cga 912 Lys Glu Leu Ser Asn Gly Trp Arg Ala Val Phe Ser Asn Pro Gly Arg     290 295 300   tgg tgg agc ggc tat ccg cgt cgc taa 939 Trp Trp Ser Gly Tyr Pro Arg Arg * 305 310   <210> 9 <211> 312 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative rhamnosyl transferase IV <400> 9 Met Gln Met Leu Pro Thr Pro Asp Val Ser Ile Leu Val Val Ala Phe  1 5 10 15 Asn Ser Thr Glu Tyr Ile Glu Asp Cys Leu Arg Gly Ile Ala Glu Gly             20 25 30 Ala Gly Lys Thr Pro His Glu Val Leu Leu Ile Asp Asn Gly Asp Gly         35 40 45 Arg Thr Glu Ala Leu Val Arg Gln Arg Phe His His Val Arg Ile Val     50 55 60 Pro Ser Glu Gly Asn Ile Gly Phe Gly Ala Gly Asn Asn Arg Leu Ala 65 70 75 80 Ala Gln Ala Ala Gly Pro Leu Leu Leu Leu Val Asn Pro Asp Ala Ile                 85 90 95 Pro Gln Pro Gly Ala Ile Asp Gln Leu Val Thr Phe Ala Lys Gln His             100 105 110 Pro Glu Ala Ala Ala Trp Gly Gly Arg Ser Tyr Ser Pro Ser Gly Asp         115 120 125 Leu Glu Pro Ala Asn Phe Met Ser Leu Pro Thr Pro Ala Asp Phe Leu     130 135 140 Thr Ala Ile Phe Asn Ala Arg Ala Leu Arg Ser Gly Gly Leu Gln Glu 145 150 155 160 Gly Ala Thr Thr Pro Gly Ala Val Glu Val Leu Asn Gly Gly Phe Met                 165 170 175 Met Val Arg Thr Asp Val Trp Gln Ala Ile Gly Gly Phe Asp Glu Ser             180 185 190 Phe Phe Leu Tyr Ser Glu Glu Ile Asp Leu Phe Gln Arg Ile Arg Thr         195 200 205 Leu Gly His Lys Val Leu Val Asp Pro Ser Val Lys Val Val His Asn     210 215 220 Thr Gly Ser Gly Gln Ser Met Ser Gln Asn Arg Leu Met Tyr Leu Thr 225 230 235 240 Thr Gly Arg Met His Tyr Ala Arg Lys His Phe Gly Ala Leu Gly Thr                 245 250 255 Leu Ala Thr Gly Cys Ala Leu Trp Leu Ile Ala Ala Lys Tyr Thr Leu             260 265 270 Val Gly Ala Ala Leu Trp Arg Leu Ser Pro Arg Thr Gly Thr Arg Tyr         275 280 285 Lys Glu Leu Ser Asn Gly Trp Arg Ala Val Phe Ser Asn Pro Gly Arg     290 295 300 Trp Trp Ser Gly Tyr Pro Arg Arg 305 310 <210> 10 <211> 972 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS <222> (1) ... (972) <223> dpsI <400> 10 atg ctg cac tgc caa cgc tat tgc gga tgc ccg ccc gtc cga ata ggt 48 Met Leu His Cys Gln Arg Tyr Cys Gly Cys Pro Pro Val Arg Ile Gly  1 5 10 15   tca agt aga agt ttg tgc cgt gcg caa ttc cgt gcc ggc ggg gag gtc 96 Ser Ser Arg Ser Leu Cys Arg Ala Gln Phe Arg Ala Gly Gly Glu Val              20 25 30   ttc atg aag aaa ttg tac ctg gtt acg gcg gtg gct gcg gcc gcg ctg 144 Phe Met Lys Lys Leu Tyr Leu Val Thr Ala Val Ala Ala Ala Ala Leu          35 40 45   gcc gtc tcc gga tgt ggc ggc aag ggc ggc aag ctc gac aag ggg cag 192 Ala Val Ser Gly Cys Gly Gly Lys Gly Gly Lys Leu Asp Lys Gly Gln      50 55 60   gtg gtc gcc agc gtc gat ggc gaa gaa atc acc gtc ttc gag ctg aat 240 Val Val Ala Ser Val Asp Gly Glu Glu Ile Thr Val Phe Glu Leu Asn  65 70 75 80   gcc gaa ctg cag gcc tcc cag gta ccc ccg ggg acc gat cgc aag ctg 288 Ala Glu Leu Gln Ala Ser Gln Val Pro Pro Gly Thr Asp Arg Lys Leu                  85 90 95   gcc gag cag ctg gcg ctg cag cgc atc atc gag cgc aag atc ctc gcc 336 Ala Glu Gln Leu Ala Leu Gln Arg Ile Ile Glu Arg Lys Ile Leu Ala             100 105 110   aag gtc gcc cgc gag cag aag ctg gac aag acg cct gcc ttc ctg atc 384 Lys Val Ala Arg Glu Gln Lys Leu Asp Lys Thr Pro Ala Phe Leu Ile         115 120 125   cag gag cgc cgg gcc gac gag ctg atc ctc acc gcc atg ctg cgc gac 432 Gln Glu Arg Arg Ala Asp Glu Leu Ile Leu Thr Ala Met Leu Arg Asp     130 135 140   aag atc gcc ggc ggc atc gcc cag ccg acc gat gcc gag atc gag aaa 480 Lys Ile Ala Gly Gly Ile Ala Gln Pro Thr Asp Ala Glu Ile Glu Lys 145 150 155 160   tat cag gcc gcg cat ccg gag cgg ttc gcg cag cgc aag atc tac gcg 528 Tyr Gln Ala Ala His Pro Glu Arg Phe Ala Gln Arg Lys Ile Tyr Ala                 165 170 175   atc gat cag gtc gtc ttc gct ccg ccg agc tcg gcc gca aag ctc aag 576 Ile Asp Gln Val Val Phe Ala Pro Pro Ser Ser Ala Ala Lys Leu Lys             180 185 190   caa ttc gcg ccg ctg aag acg ctg gac cag cta acc gcc aag ctc tcg 624 Gln Phe Ala Pro Leu Lys Thr Leu Asp Gln Leu Thr Ala Lys Leu Ser         195 200 205   gcg gac aat gtc cag ttc cgt cgc gcg ccg tcg cag atc gac acc gct 672 Ala Asp Asn Val Gln Phe Arg Arg Ala Pro Ser Gln Ile Asp Thr Ala     210 215 220   gcg ctg ccg ccg gaa atc gct gcc aag atc gcg tcg ctg ccg gca cag 720 Ala Leu Pro Pro Glu Ile Ala Ala Lys Ile Ala Ser Leu Pro Ala Gln 225 230 235 240   gag atg ttc atc ctg ccg acc cag cag gga ctg acc gcg aat atc atc 768 Glu Met Phe Ile Leu Pro Thr Gln Gln Gly Leu Thr Ala Asn Ile Ile                 245 250 255   acg tcg acc acg gtg ctg ccg gtg ccg gcc gac cag gcg cgc gag atc 816 Thr Ser Thr Thr Val Leu Pro Val Pro Ala Asp Gln Ala Arg Glu Ile             260 265 270   gcg ctc agc ggg ctg cgt acc gag cgc ttc ggc aag gcg gct gac gca 864 Ala Leu Ser Gly Leu Arg Thr Glu Arg Phe Gly Lys Ala Ala Asp Ala         275 280 285   cag ctc aac gac cgc ctg aag aag gcg cgg gaa acc gtg aaa tat cag 912 Gln Leu Asn Asp Arg Leu Lys Lys Ala Arg Glu Thr Val Lys Tyr Gln     290 295 300   gcc ggc tac agc gca ccg ccg cag ctt cgc ggc agc ggc gca acg ccg 960 Ala Gly Tyr Ser Ala Pro Pro Gln Leu Arg Gly Ser Gly Ala Thr Pro 305 310 315 320   gcg ggg aac tga 972 Ala Gly Asn *   <210> 11 <211> 323 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> unknown <400> 11 Met Leu His Cys Gln Arg Tyr Cys Gly Cys Pro Pro Val Arg Ile Gly  1 5 10 15 Ser Ser Arg Ser Leu Cys Arg Ala Gln Phe Arg Ala Gly Gly Glu Val             20 25 30 Phe Met Lys Lys Leu Tyr Leu Val Thr Ala Val Ala Ala Ala Ala Leu         35 40 45 Ala Val Ser Gly Cys Gly Gly Lys Gly Gly Lys Leu Asp Lys Gly Gln     50 55 60 Val Val Ala Ser Val Asp Gly Glu Glu Ile Thr Val Phe Glu Leu Asn 65 70 75 80 Ala Glu Leu Gln Ala Ser Gln Val Pro Pro Gly Thr Asp Arg Lys Leu                 85 90 95 Ala Glu Gln Leu Ala Leu Gln Arg Ile Ile Glu Arg Lys Ile Leu Ala             100 105 110 Lys Val Ala Arg Glu Gln Lys Leu Asp Lys Thr Pro Ala Phe Leu Ile         115 120 125 Gln Glu Arg Arg Ala Asp Glu Leu Ile Leu Thr Ala Met Leu Arg Asp     130 135 140 Lys Ile Ala Gly Gly Ile Ala Gln Pro Thr Asp Ala Glu Ile Glu Lys 145 150 155 160 Tyr Gln Ala Ala His Pro Glu Arg Phe Ala Gln Arg Lys Ile Tyr Ala                 165 170 175 Ile Asp Gln Val Val Phe Ala Pro Pro Ser Ser Ala Ala Lys Leu Lys             180 185 190 Gln Phe Ala Pro Leu Lys Thr Leu Asp Gln Leu Thr Ala Lys Leu Ser         195 200 205 Ala Asp Asn Val Gln Phe Arg Arg Ala Pro Ser Gln Ile Asp Thr Ala     210 215 220 Ala Leu Pro Pro Glu Ile Ala Ala Lys Ile Ala Ser Leu Pro Ala Gln 225 230 235 240 Glu Met Phe Ile Leu Pro Thr Gln Gln Gly Leu Thr Ala Asn Ile Ile                 245 250 255 Thr Ser Thr Thr Val Leu Pro Val Pro Ala Asp Gln Ala Arg Glu Ile             260 265 270 Ala Leu Ser Gly Leu Arg Thr Glu Arg Phe Gly Lys Ala Ala Asp Ala         275 280 285 Gln Leu Asn Asp Arg Leu Lys Lys Ala Arg Glu Thr Val Lys Tyr Gln     290 295 300 Ala Gly Tyr Ser Ala Pro Pro Gln Leu Arg Gly Ser Gly Ala Thr Pro 305 310 315 320 Ala gly asn <210> 12 <211> 1047 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (1047) <223> dpsK <400> 12 atg gca gaa gcg aac gcg gta gat gga aag gcc tcc aag ccg ctg aaa 48 Met Ala Glu Ala Asn Ala Val Asp Gly Lys Ala Ser Lys Pro Leu Lys  1 5 10 15   atg tgc ctt gca gcg tcg ggc ggc ggc cat ctc cgg caa atc ctc gat 96 Met Cys Leu Ala Ala Ser Gly Gly Gly His Leu Arg Gln Ile Leu Asp              20 25 30   ctg gaa tcg gtg tgg cgc gaa cac gat tat ttc ttc gtt act gaa gat 144 Leu Glu Ser Val Trp Arg Glu His Asp Tyr Phe Phe Val Thr Glu Asp          35 40 45   acc gcg ctc ggc cgg agc ctt gcc gaa aaa cat ccc gtc gaa ctg gtg 192 Thr Ala Leu Gly Arg Ser Leu Ala Glu Lys His Pro Val Glu Leu Val      50 55 60   gag cac tat gcg ctc ggc cag gcc aag ctg ggc cat ccc ttg cgc atg 240 Glu His Tyr Ala Leu Gly Gln Ala Lys Leu Gly His Pro Leu Arg Met  65 70 75 80   ctg ggc ggc gca tgg cgc aac ctg cgc cag agc ctt tcg atc ctg cgc 288 Leu Gly Gly Ala Trp Arg Asn Leu Arg Gln Ser Leu Ser Ile Leu Arg                  85 90 95   cgg cac aag ccg gat gtg gtg att tcc acc ggc gcg ggc gca gtc tat 336 Arg His Lys Pro Asp Val Val Ile Ser Thr Gly Ala Gly Ala Val Tyr             100 105 110   ttc acc gcg ctg ctc gcc aaa ctg tcg ggc gcc aag ttc gtc cat atc 384 Phe Thr Ala Leu Leu Ala Lys Leu Ser Gly Ala Lys Phe Val His Ile         115 120 125   gaa agc ttc gcg cgc ttc gac cac ccg tct gcc ttc ggc aag atg gtg 432 Glu Ser Phe Ala Arg Phe Asp His Pro Ser Ala Phe Gly Lys Met Val     130 135 140   aag ggc atc gcg acg gtg acg atc gtc cag tcg gcg gcg ctg aaa gaa 480 Lys Gly Ile Ala Thr Val Thr Ile Val Gln Ser Ala Ala Leu Lys Glu 145 150 155 160   acc tgg cct gat gcc gag ctg ttc gat ccg ttc cgc ctg ctc gat aca 528 Thr Trp Pro Asp Ala Glu Leu Phe Asp Pro Phe Arg Leu Leu Asp Thr                 165 170 175   ccg cgc ccg ccc aag cag gcg cta atc ttc gcg acg gtc ggc gcc acc 576 Pro Arg Pro Pro Lys Gln Ala Leu Ile Phe Ala Thr Val Gly Ala Thr             180 185 190   ctg ccc ttc ccg cgg ctg gtg cag gca gtg ctc gac ctg aag cgc gcc 624 Leu Pro Phe Pro Arg Leu Val Gln Ala Val Leu Asp Leu Lys Arg Ala         195 200 205   ggc ggg ctg ccg ggc aag ctg atc ctg caa tat ggc gac cag gac ctg 672 Gly Gly Leu Pro Gly Lys Leu Ile Leu Gln Tyr Gly Asp Gln Asp Leu     210 215 220   ccc gat ccc ggc atc ccc gac gtc gag atc cgc cgt acc atc ccg ttc 720 Pro Asp Pro Gly Ile Pro Asp Val Glu Ile Arg Arg Thr Ile Pro Phe 225 230 235 240   gac gat ctg cag ctg ctg ctg cgc gat gcg gat atg gtg ata tgc cac 768 Asp Asp Leu Gln Leu Leu Leu Arg Asp Ala Asp Met Val Ile Cys His                 245 250 255   ggc ggc acc gga tcg ctg gtc acg gcg ctg cgc gcc ggc tgc cgg gtc 816 Gly Gly Thr Gly Ser Leu Val Thr Ala Leu Arg Ala Gly Cys Arg Val             260 265 270   gtc gcc ttt ccg cgc cgc cac gat ctg ggc gag cat tat gac gat cac 864 Val Ala Phe Pro Arg Arg His Asp Leu Gly Glu His Tyr Asp Asp His         275 280 285   cag gaa gag atc gcc cag acc ttc gcc gac cgg ggc ctg ctc cag gcg 912 Gln Glu Glu Ile Ala Gln Thr Phe Ala Asp Arg Gly Leu Leu Gln Ala     290 295 300   gtg cgc gac gag cgc cag ctc ggc gcc gct gtg gaa gcg gcc aag gca 960 Val Arg Asp Glu Arg Gln Leu Gly Ala Ala Val Glu Ala Ala Lys Ala 305 310 315 320   acc gag ccg cag ctg gcg acc acc gac cac acg gcc ctc gcg gcg cgg 1008 Thr Glu Pro Gln Leu Ala Thr Thr Asp His Thr Ala Leu Ala Ala Arg                 325 330 335   ctg cgc cag ctg ctg gcg cag tgg agt gcc aag cga tga 1047 Leu Arg Gln Leu Leu Ala Gln Trp Ser Ala Lys Arg *             340 345   <210> 13 <211> 348 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> beta-1,4-glucuronosyl transferase II <400> 13 Met Ala Glu Ala Asn Ala Val Asp Gly Lys Ala Ser Lys Pro Leu Lys  1 5 10 15 Met Cys Leu Ala Ala Ser Gly Gly Gly His Leu Arg Gln Ile Leu Asp             20 25 30 Leu Glu Ser Val Trp Arg Glu His Asp Tyr Phe Phe Val Thr Glu Asp         35 40 45 Thr Ala Leu Gly Arg Ser Leu Ala Glu Lys His Pro Val Glu Leu Val     50 55 60 Glu His Tyr Ala Leu Gly Gln Ala Lys Leu Gly His Pro Leu Arg Met 65 70 75 80 Leu Gly Gly Ala Trp Arg Asn Leu Arg Gln Ser Leu Ser Ile Leu Arg                 85 90 95 Arg His Lys Pro Asp Val Val Ile Ser Thr Gly Ala Gly Ala Val Tyr             100 105 110 Phe Thr Ala Leu Leu Ala Lys Leu Ser Gly Ala Lys Phe Val His Ile         115 120 125 Glu Ser Phe Ala Arg Phe Asp His Pro Ser Ala Phe Gly Lys Met Val     130 135 140 Lys Gly Ile Ala Thr Val Thr Ile Val Gln Ser Ala Ala Leu Lys Glu 145 150 155 160 Thr Trp Pro Asp Ala Glu Leu Phe Asp Pro Phe Arg Leu Leu Asp Thr                 165 170 175 Pro Arg Pro Pro Lys Gln Ala Leu Ile Phe Ala Thr Val Gly Ala Thr             180 185 190 Leu Pro Phe Pro Arg Leu Val Gln Ala Val Leu Asp Leu Lys Arg Ala         195 200 205 Gly Gly Leu Pro Gly Lys Leu Ile Leu Gln Tyr Gly Asp Gln Asp Leu     210 215 220 Pro Asp Pro Gly Ile Pro Asp Val Glu Ile Arg Arg Thr Ile Pro Phe 225 230 235 240 Asp Asp Leu Gln Leu Leu Leu Arg Asp Ala Asp Met Val Ile Cys His                 245 250 255 Gly Gly Thr Gly Ser Leu Val Thr Ala Leu Arg Ala Gly Cys Arg Val             260 265 270 Val Ala Phe Pro Arg Arg His Asp Leu Gly Glu His Tyr Asp Asp His         275 280 285 Gln Glu Glu Ile Ala Gln Thr Phe Ala Asp Arg Gly Leu Leu Gln Ala     290 295 300 Val Arg Asp Glu Arg Gln Leu Gly Ala Ala Val Glu Ala Ala Lys Ala 305 310 315 320 Thr Glu Pro Gln Leu Ala Thr Thr Asp His Thr Ala Leu Ala Ala Arg                 325 330 335 Leu Arg Gln Leu Leu Ala Gln Trp Ser Ala Lys Arg             340 345 <210> 14 <211> 867 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (867) <223> dpsL <400> 14 atg agc acg ccc cgg atc agc gtc gtc atc ccg cac tat aac gat ccg 48 Met Ser Thr Pro Arg Ile Ser Val Val Ile Pro His Tyr Asn Asp Pro  1 5 10 15   caa tcc ttg cgg ctc tgc ctg gat gcg ctg gag cgg cag acg atc ggt 96 Gln Ser Leu Arg Leu Cys Leu Asp Ala Leu Glu Arg Gln Thr Ile Gly              20 25 30   cgc gac gcg ttc gag atc atc gtc ggc gac aac aat tcg ccc tgt ggg 144 Arg Asp Ala Phe Glu Ile Ile Val Gly Asp Asn Asn Ser Pro Cys Gly          35 40 45   ctc gcg gcg gtg gag gcg gcg gtc gcc gga cgt gcg cgg atc gtg acc 192 Leu Ala Ala Val Glu Ala Ala Val Ala Gly Arg Ala Arg Ile Val Thr      50 55 60   att ctg gaa aag ggg gcg ggc ccc gcg cgc aac ggg gcg gca gcc gca 240 Ile Leu Glu Lys Gly Ala Gly Pro Ala Arg Asn Gly Ala Ala Ala Ala  65 70 75 80   gcg cgt ggc gag atc ctc gcc ttt acc gac agt gac tgc gtg gtg gag 288 Ala Arg Gly Glu Ile Leu Ala Phe Thr Asp Ser Asp Cys Val Val Glu                  85 90 95   ccc ggc tgg ctg gcg ggc ggc acg acc agg gtc gcg cct ggc cgt ttc 336 Pro Gly Trp Leu Ala Gly Gly Thr Thr Arg Val Ala Pro Gly Arg Phe             100 105 110   atc ggc ggg cac atg tat gtg cgc aag ccc gaa ggg ccg ccg aac ggc 384 Ile Gly Gly His Met Tyr Val Arg Lys Pro Glu Gly Pro Pro Asn Gly         115 120 125   gcc gag gcg ctg gag atg gcg ctg gcg ttc gac aat gaa ggc tat gtg 432 Ala Glu Ala Leu Glu Met Ala Leu Ala Phe Asp Asn Glu Gly Tyr Val     130 135 140   cgg cgc acc cag ttc acg gtc acc gca aac ctg ttc gtg atg cgc gcc 480 Arg Arg Thr Gln Phe Thr Val Thr Ala Asn Leu Phe Val Met Arg Ala 145 150 155 160   gat ttc gaa cgg gtc ggc ggc ttc cgc gtt ggc gtg tcc gag gat ctg 528 Asp Phe Glu Arg Val Gly Gly Phe Arg Val Gly Val Ser Glu Asp Leu                 165 170 175   gaa tgg tgc cac cgg gcg atc gcc agc ggc ctc acc atc aac tat gca 576 Glu Trp Cys His Arg Ala Ile Ala Ser Gly Leu Thr Ile Asn Tyr Ala             180 185 190   ccg gat gca tcg gtg ggc cac ccg ccc cgg ccc gac tgg tcg gcc ctg 624 Pro Asp Ala Ser Val Gly His Pro Pro Arg Pro Asp Trp Ser Ala Leu         195 200 205   ctg gtg aag acg cgg cgc atc cag cgc gaa ctc tat ctg ttc aac atc 672 Leu Val Lys Thr Arg Arg Ile Gln Arg Glu Leu Tyr Leu Phe Asn Ile     210 215 220   gag cgg ccg aag ggc agg ctg cgc tgg ctg gtc cgt tcc gtg gcg caa 720 Glu Arg Pro Lys Gly Arg Leu Arg Trp Leu Val Arg Ser Val Ala Gln 225 230 235 240   ccg gcg atg atc cca cag gac gtg gcc aag atc ctg cgc aca ccg ggt 768 Pro Ala Met Ile Pro Gln Asp Val Ala Lys Ile Leu Arg Thr Pro Gly                 245 250 255   acc aag ggc gcg cgc ctc gct gcg gtc acc acg ctg gtc cgg ctg cgg 816 Thr Lys Gly Ala Arg Leu Ala Ala Val Thr Thr Leu Val Arg Leu Arg             260 265 270   ctg tgg cgc ggc ggc gcc ggc ttg ttg cag ttg ctc ggc cgc gac atc 864 Leu Trp Arg Gly Gly Ala Gly Leu Leu Gln Leu Leu Gly Arg Asp Ile         275 280 285   tga 867  *   <210> 15 <211> 288 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> glucosyl transferase III <400> 15 Met Ser Thr Pro Arg Ile Ser Val Val Ile Pro His Tyr Asn Asp Pro  1 5 10 15 Gln Ser Leu Arg Leu Cys Leu Asp Ala Leu Glu Arg Gln Thr Ile Gly             20 25 30 Arg Asp Ala Phe Glu Ile Ile Val Gly Asp Asn Asn Ser Pro Cys Gly         35 40 45 Leu Ala Ala Val Glu Ala Ala Val Ala Gly Arg Ala Arg Ile Val Thr     50 55 60 Ile Leu Glu Lys Gly Ala Gly Pro Ala Arg Asn Gly Ala Ala Ala Ala 65 70 75 80 Ala Arg Gly Glu Ile Leu Ala Phe Thr Asp Ser Asp Cys Val Val Glu                 85 90 95 Pro Gly Trp Leu Ala Gly Gly Thr Thr Arg Val Ala Pro Gly Arg Phe             100 105 110 Ile Gly Gly His Met Tyr Val Arg Lys Pro Glu Gly Pro Pro Asn Gly         115 120 125 Ala Glu Ala Leu Glu Met Ala Leu Ala Phe Asp Asn Glu Gly Tyr Val     130 135 140 Arg Arg Thr Gln Phe Thr Val Thr Ala Asn Leu Phe Val Met Arg Ala 145 150 155 160 Asp Phe Glu Arg Val Gly Gly Phe Arg Val Gly Val Ser Glu Asp Leu                 165 170 175 Glu Trp Cys His Arg Ala Ile Ala Ser Gly Leu Thr Ile Asn Tyr Ala             180 185 190 Pro Asp Ala Ser Val Gly His Pro Pro Arg Pro Asp Trp Ser Ala Leu         195 200 205 Leu Val Lys Thr Arg Arg Ile Gln Arg Glu Leu Tyr Leu Phe Asn Ile     210 215 220 Glu Arg Pro Lys Gly Arg Leu Arg Trp Leu Val Arg Ser Val Ala Gln 225 230 235 240 Pro Ala Met Ile Pro Gln Asp Val Ala Lys Ile Leu Arg Thr Pro Gly                 245 250 255 Thr Lys Gly Ala Arg Leu Ala Ala Val Thr Thr Leu Val Arg Leu Arg             260 265 270 Leu Trp Arg Gly Gly Ala Gly Leu Leu Gln Leu Leu Gly Arg Asp Ile         275 280 285 <210> 16 <211> 1389 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (1389) <223> dpsJ <400> 16 gtg gct gta ggt tcc gcg ctg aga ttc ttg tgg ccg ttc ggc cgt cga 48 Val Ala Val Gly Ser Ala Leu Arg Phe Leu Trp Pro Phe Gly Arg Arg  1 5 10 15   gaa gaa cct gaa gaa gag ggc tat ttc ccg ctg act gcg aca gtg gtg 96 Glu Glu Pro Glu Glu Glu Gly Tyr Phe Pro Leu Thr Ala Thr Val Val              20 25 30   ccg cat cgc gat gcc cat agc ggc cgt ggt cgc ccg gat ttc cca acc 144 Pro His Arg Asp Ala His Ser Gly Arg Gly Arg Pro Asp Phe Pro Thr          35 40 45   ttc cgt gcc tcc gcg ctg gac cgc ccg ctg gat cgc cgc cgc gac gag 192 Phe Arg Ala Ser Ala Leu Asp Arg Pro Leu Asp Arg Arg Arg Asp Glu      50 55 60   cgc cgc gag atc acg cgc gcc cgg ttc gcg ctg gcg acc ttc ttc aca 240 Arg Arg Glu Ile Thr Arg Ala Arg Phe Ala Leu Ala Thr Phe Phe Thr  65 70 75 80   ccc acc cag ccg gtg gcc gat cgg tcg agc ttc gcc ggg cgc ctc ggc 288 Pro Thr Gln Pro Val Ala Asp Arg Ser Ser Phe Ala Gly Arg Leu Gly                  85 90 95   gtg ctg gcg cgc cta atc tcc tcg atc gag agc cag cgc agc cat gtc 336 Val Leu Ala Arg Leu Ile Ser Ser Ile Glu Ser Gln Arg Ser His Val             100 105 110   gtg ctc tat ggc gag cgc ggc atc ggc aag acc tcg ctg ctc cac gtg 384 Val Leu Tyr Gly Glu Arg Gly Ile Gly Lys Thr Ser Leu Leu His Val         115 120 125   ctg acc gat gtc gcc cgc gaa tcc agc tat atc gtc agc tat gcg acc 432 Leu Thr Asp Val Ala Arg Glu Ser Ser Tyr Ile Val Ser Tyr Ala Thr     130 135 140   tgc ggt gcg aac gcg aat ttc agc gat gtc ttc cgc gcc gtg ctg gaa 480 Cys Gly Ala Asn Ala Asn Phe Ser Asp Val Phe Arg Ala Val Leu Glu 145 150 155 160   gac gtg ccg ctg ctg ttc cat cgc ggc gtg gcg ccc aac gcc ggc gag 528 Asp Val Pro Leu Leu Phe His Arg Gly Val Ala Pro Asn Ala Gly Glu                 165 170 175   gcg gag agc ggc ggc aac ttg gcc gac cgc ctg ccg acg ggc agc ttc 576 Ala Glu Ser Gly Gly Asn Leu Ala Asp Arg Leu Pro Thr Gly Ser Phe             180 185 190   ggg ccc ggc gaa ctg gcc gac ctg tgc gcc gac atc acg ggc aca cgc 624 Gly Pro Gly Glu Leu Ala Asp Leu Cys Ala Asp Ile Thr Gly Thr Arg         195 200 205   gtg ctg atc atc ctc gac gaa tat gat cgc gtc agc gat tcc gcc ttc 672 Val Leu Ile Ile Leu Asp Glu Tyr Asp Arg Val Ser Asp Ser Ala Phe     210 215 220   cgt cag cag gtc gcc gag ctg atc aag aac ctg tcg gac cgt tcg gcg 720 Arg Gln Gln Val Ala Glu Leu Ile Lys Asn Leu Ser Asp Arg Ser Ala 225 230 235 240   cgc gtc cag ctg gtg atc gcg ggc gtc gcc tcg aac ctg cag gag ctg 768 Arg Val Gln Leu Val Ile Ala Gly Val Ala Ser Asn Leu Gln Glu Leu                 245 250 255   atc ggt tat gcg ccg tcg atc cgc cgc aac gtc atc ggc ctg ccg atg 816 Ile Gly Tyr Ala Pro Ser Ile Arg Arg Asn Val Ile Gly Leu Pro Met             260 265 270   ccc cgg ctg gag gaa tcg gag gtg cag gag atg atc gcg ctc ggc gaa 864 Pro Arg Leu Glu Glu Ser Glu Val Gln Glu Met Ile Ala Leu Gly Glu         275 280 285   acc gcc tcg ggc gtt cgc ttc gat ccg gac ctg act cac atg atc cac 912 Thr Ala Ser Gly Val Arg Phe Asp Pro Asp Leu Thr His Met Ile His     290 295 300   ctg ctc gcg ctg ggg tcg ccc tat ttc gcg cgg ctg ctg tgc cac cat 960 Leu Leu Ala Leu Gly Ser Pro Tyr Phe Ala Arg Leu Leu Cys His His 305 310 315 320   tcc gcg ctg gaa gcc ctg gac cag ggc cgc ctc acg gtc gac gcc ggg 1008 Ser Ala Leu Glu Ala Leu Asp Gln Gly Arg Leu Thr Val Asp Ala Gly                 325 330 335   cat ctg cgt cgt gcg ctc gac cag gcg atc ctt gag atc gag ggc cgc 1056 His Leu Arg Arg Ala Leu Asp Gln Ala Ile Leu Glu Ile Glu Gly Arg             340 345 350   atg ccg ccg cgc gcg gtg atc gag atg cgc aag ttc gtc ggc ggc cgc 1104 Met Pro Pro Arg Ala Val Ile Glu Met Arg Lys Phe Val Gly Gly Arg         355 360 365   tac gat cca ctc gtc gcg gcg ctg ggc gag gcc tcg cgc tcg gcg gat 1152 Tyr Asp Pro Leu Val Ala Ala Leu Gly Glu Ala Ser Arg Ser Ala Asp     370 375 380   ggc tgg ttc agc ggc caa gcc gtg gtg gat ctg ctg ccg ggc gcg cac 1200 Gly Trp Phe Ser Gly Gln Ala Val Val Asp Leu Leu Pro Gly Ala His 385 390 395 400   atc acg gcg gcg cag gtc gag cag gag ctg ggc gag ctc acc ggt caa 1248 Ile Thr Ala Ala Gln Val Glu Gln Glu Leu Gly Glu Leu Thr Gly Gln                 405 410 415   ctc ggc ctc gaa tcc gaa acg cag gac ggc gat tgc cgc ttc cgc ttc 1296 Leu Gly Leu Glu Ser Glu Thr Gln Asp Gly Asp Cys Arg Phe Arg Phe             420 425 430   acc gac gat acg ctg ccg gtc tat ctg tgg ctg atg atc ggc cgc ctg 1344 Thr Asp Asp Thr Leu Pro Val Tyr Leu Trp Leu Met Ile Gly Arg Leu         435 440 445   cgg ctc gac agc ggc acg ctg gaa gac gcg ctg gcc acc gtc tga 1389 Arg Leu Asp Ser Gly Thr Leu Glu Asp Ala Leu Ala Thr Val *     450 455 460   <210> 17 <211> 462 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> unknown <400> 17 Val Ala Val Gly Ser Ala Leu Arg Phe Leu Trp Pro Phe Gly Arg Arg  1 5 10 15 Glu Glu Pro Glu Glu Glu Gly Tyr Phe Pro Leu Thr Ala Thr Val Val             20 25 30 Pro His Arg Asp Ala His Ser Gly Arg Gly Arg Pro Asp Phe Pro Thr         35 40 45 Phe Arg Ala Ser Ala Leu Asp Arg Pro Leu Asp Arg Arg Arg Asp Glu     50 55 60 Arg Arg Glu Ile Thr Arg Ala Arg Phe Ala Leu Ala Thr Phe Phe Thr 65 70 75 80 Pro Thr Gln Pro Val Ala Asp Arg Ser Ser Phe Ala Gly Arg Leu Gly                 85 90 95 Val Leu Ala Arg Leu Ile Ser Ser Ile Glu Ser Gln Arg Ser His Val             100 105 110 Val Leu Tyr Gly Glu Arg Gly Ile Gly Lys Thr Ser Leu Leu His Val         115 120 125 Leu Thr Asp Val Ala Arg Glu Ser Ser Tyr Ile Val Ser Tyr Ala Thr     130 135 140 Cys Gly Ala Asn Ala Asn Phe Ser Asp Val Phe Arg Ala Val Leu Glu 145 150 155 160 Asp Val Pro Leu Leu Phe His Arg Gly Val Ala Pro Asn Ala Gly Glu                 165 170 175 Ala Glu Ser Gly Gly Asn Leu Ala Asp Arg Leu Pro Thr Gly Ser Phe             180 185 190 Gly Pro Gly Glu Leu Ala Asp Leu Cys Ala Asp Ile Thr Gly Thr Arg         195 200 205 Val Leu Ile Ile Leu Asp Glu Tyr Asp Arg Val Ser Asp Ser Ala Phe     210 215 220 Arg Gln Gln Val Ala Glu Leu Ile Lys Asn Leu Ser Asp Arg Ser Ala 225 230 235 240 Arg Val Gln Leu Val Ile Ala Gly Val Ala Ser Asn Leu Gln Glu Leu                 245 250 255 Ile Gly Tyr Ala Pro Ser Ile Arg Arg Asn Val Ile Gly Leu Pro Met             260 265 270 Pro Arg Leu Glu Glu Ser Glu Val Gln Glu Met Ile Ala Leu Gly Glu         275 280 285 Thr Ala Ser Gly Val Arg Phe Asp Pro Asp Leu Thr His Met Ile His     290 295 300 Leu Leu Ala Leu Gly Ser Pro Tyr Phe Ala Arg Leu Leu Cys His His 305 310 315 320 Ser Ala Leu Glu Ala Leu Asp Gln Gly Arg Leu Thr Val Asp Ala Gly                 325 330 335 His Leu Arg Arg Ala Leu Asp Gln Ala Ile Leu Glu Ile Glu Gly Arg             340 345 350 Met Pro Pro Arg Ala Val Ile Glu Met Arg Lys Phe Val Gly Gly Arg         355 360 365 Tyr Asp Pro Leu Val Ala Ala Leu Gly Glu Ala Ser Arg Ser Ala Asp     370 375 380 Gly Trp Phe Ser Gly Gln Ala Val Val Asp Leu Leu Pro Gly Ala His 385 390 395 400 Ile Thr Ala Ala Gln Val Glu Gln Glu Leu Gly Glu Leu Thr Gly Gln                 405 410 415 Leu Gly Leu Glu Ser Glu Thr Gln Asp Gly Asp Cys Arg Phe Arg Phe             420 425 430 Thr Asp Asp Thr Leu Pro Val Tyr Leu Trp Leu Met Ile Gly Arg Leu         435 440 445 Arg Leu Asp Ser Gly Thr Leu Glu Asp Ala Leu Ala Thr Val     450 455 460 <210> 18 <211> 1299 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (1299) <223> dpsF <400> 18 atg aag ccg aga ccc ggg gga acc ttt atg caa gta aat ttc aat cga 48 Met Lys Pro Arg Pro Gly Gly Thr Phe Met Gln Val Asn Phe Asn Arg  1 5 10 15   cag gct cgc aag ctc ggt gcc ggc aat gcg ctc gcg cgg ggg ggg ccc 96 Gln Ala Arg Lys Leu Gly Ala Gly Asn Ala Leu Ala Arg Gly Gly Pro              20 25 30   gtg ctt gcg ctg ctt gcg acc gcg gca tgg aca caa cct gcg ctg gcg 144 Val Leu Ala Leu Leu Ala Thr Ala Ala Trp Thr Gln Pro Ala Leu Ala          35 40 45   cag cga cag gca ttt gag tcc cgc ccc tcc ggt agc gag cga cag gtc 192 Gln Arg Gln Ala Phe Glu Ser Arg Pro Ser Gly Ser Glu Arg Gln Val      50 55 60   gat att cgc gcg acg ggg tcg ctg gaa tat gac gac aac gtc gtg ctg 240 Asp Ile Arg Ala Thr Gly Ser Leu Glu Tyr Asp Asp Asn Val Val Leu  65 70 75 80   aac gac cag cgg atc acg gac ggc gcg cgt ggc gat gtg atc gca tcg 288 Asn Asp Gln Arg Ile Thr Asp Gly Ala Arg Gly Asp Val Ile Ala Ser                  85 90 95   ccc ggg ctg gac gtg acc cta gtt ctg ccc cgc gcc acc ggg cag ctc 336 Pro Gly Leu Asp Val Thr Leu Val Leu Pro Arg Ala Thr Gly Gln Leu             100 105 110   tac ctc acc ggc aat gtc gga tat cgc ttt tac aag cga tat acc aac 384 Tyr Leu Thr Gly Asn Val Gly Tyr Arg Phe Tyr Lys Arg Tyr Thr Asn         115 120 125   ttt aac cgc gag cag atc tcg ctc acc ggc ggc gca gat cag cgg ttc 432 Phe Asn Arg Glu Gln Ile Ser Leu Thr Gly Gly Ala Asp Gln Arg Phe     130 135 140   gcc tcc tgc gtc gtg cac ggg gaa gtc ggc tat cag cgc cac ctc acc 480 Ala Ser Cys Val Val His Gly Glu Val Gly Tyr Gln Arg His Leu Thr 145 150 155 160   gac ctg tcc agc atc ttg atc cag gac acc acg cct gcg ctc aac aac 528 Asp Leu Ser Ser Ile Leu Ile Gln Asp Thr Thr Pro Ala Leu Asn Asn                 165 170 175   acc gaa gag gcc cgg cag tac acc gcg gat atc ggc tgc ggc gcg acc 576 Thr Glu Glu Ala Arg Gln Tyr Thr Ala Asp Ile Gly Cys Gly Ala Thr             180 185 190   tac ggc ctg cgg cct gcc gtt tcc tac acc cgc aac gaa gtg cgc aac 624 Tyr Gly Leu Arg Pro Ala Val Ser Tyr Thr Arg Asn Glu Val Arg Asn         195 200 205   agc ctt gcc gag cgc cga tac gcg gac tcg aat acc aac acc ttt acc 672 Ser Leu Ala Glu Arg Arg Tyr Ala Asp Ser Asn Thr Asn Thr Phe Thr     210 215 220   gca cag ctt ggc ctg act tcg cct gcc ctg ggg acc gtg gcg gta ttt 720 Ala Gln Leu Gly Leu Thr Ser Pro Ala Leu Gly Thr Val Ala Val Phe 225 230 235 240   ggg cgt atg tcc gac agc agc tat gtc cat cgc gtc ctt ccc ggc att 768 Gly Arg Met Ser Asp Ser Ser Tyr Val His Arg Val Leu Pro Gly Ile                 245 250 255   acc ggc cag gac ggg atg aag agc tac gcg gcc ggc gtc cag ctc gag 816 Thr Gly Gln Asp Gly Met Lys Ser Tyr Ala Ala Gly Val Gln Leu Glu             260 265 270   cgc tcg gtg gcc aac cga ctc cat ttc aac ggc tcg gtg aat tac acc 864 Arg Ser Val Ala Asn Arg Leu His Phe Asn Gly Ser Val Asn Tyr Thr         275 280 285   gag gtt gac cca aag ctc gca tcc acc aaa gga ttc aag ggc gta gga 912 Glu Val Asp Pro Lys Leu Ala Ser Thr Lys Gly Phe Lys Gly Val Gly     290 295 300   ttt aac gtt tcc ggc gat tat gct ggt gat cag tac agc ctc caa ttg 960 Phe Asn Val Ser Gly Asp Tyr Ala Gly Asp Gln Tyr Ser Leu Gln Leu 305 310 315 320   ctg gct tca cga tcg ccc cag cct tca ctt ctt ctg ttc gtg ggt tac 1008 Leu Ala Ser Arg Ser Pro Gln Pro Ser Leu Leu Leu Phe Val Gly Tyr                 325 330 335   gag att gtg aca gcg gtt tcg gcg aat gcg acg cgc cgg ctg agc gat 1056 Glu Ile Val Thr Ala Val Ser Ala Asn Ala Thr Arg Arg Leu Ser Asp             340 345 350   cgc att cag ata tcg ctg caa ggc agc cga acc tgg cgc gag ctc gcg 1104 Arg Ile Gln Ile Ser Leu Gln Gly Ser Arg Thr Trp Arg Glu Leu Ala         355 360 365   tct tcg cgg ctg ctc acc aac gtg ccg att tcc ggc aac gac aac acc 1152 Ser Ser Arg Leu Leu Thr Asn Val Pro Ile Ser Gly Asn Asp Asn Thr     370 375 380   tcg acg ttg ttc gcc tcc gct acc ttc cgg ccg aat cgc cgg ctg agc 1200 Ser Thr Leu Phe Ala Ser Ala Thr Phe Arg Pro Asn Arg Arg Leu Ser 385 390 395 400   ttt gtg ctg ggt gcc ggc ctt cag cgg cgc acc agc aac acg cag cta 1248 Phe Val Leu Gly Ala Gly Leu Gln Arg Arg Thr Ser Asn Thr Gln Leu                 405 410 415   tac agt tac agc tcc aaa cgc atc aat ctc tcg acg tcg ctt tcg ctc 1296 Tyr Ser Tyr Ser Ser Lys Arg Ile Asn Leu Ser Thr Ser Leu Ser Leu             420 425 430   tga 1299  *   <210> 19 <211> 432 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> unknown <400> 19 Met Lys Pro Arg Pro Gly Gly Thr Phe Met Gln Val Asn Phe Asn Arg  1 5 10 15 Gln Ala Arg Lys Leu Gly Ala Gly Asn Ala Leu Ala Arg Gly Gly Pro             20 25 30 Val Leu Ala Leu Leu Ala Thr Ala Ala Trp Thr Gln Pro Ala Leu Ala         35 40 45 Gln Arg Gln Ala Phe Glu Ser Arg Pro Ser Gly Ser Glu Arg Gln Val     50 55 60 Asp Ile Arg Ala Thr Gly Ser Leu Glu Tyr Asp Asp Asn Val Val Leu 65 70 75 80 Asn Asp Gln Arg Ile Thr Asp Gly Ala Arg Gly Asp Val Ile Ala Ser                 85 90 95 Pro Gly Leu Asp Val Thr Leu Val Leu Pro Arg Ala Thr Gly Gln Leu             100 105 110 Tyr Leu Thr Gly Asn Val Gly Tyr Arg Phe Tyr Lys Arg Tyr Thr Asn         115 120 125 Phe Asn Arg Glu Gln Ile Ser Leu Thr Gly Gly Ala Asp Gln Arg Phe     130 135 140 Ala Ser Cys Val Val His Gly Glu Val Gly Tyr Gln Arg His Leu Thr 145 150 155 160 Asp Leu Ser Ser Ile Leu Ile Gln Asp Thr Thr Pro Ala Leu Asn Asn                 165 170 175 Thr Glu Glu Ala Arg Gln Tyr Thr Ala Asp Ile Gly Cys Gly Ala Thr             180 185 190 Tyr Gly Leu Arg Pro Ala Val Ser Tyr Thr Arg Asn Glu Val Arg Asn         195 200 205 Ser Leu Ala Glu Arg Arg Tyr Ala Asp Ser Asn Thr Asn Thr Phe Thr     210 215 220 Ala Gln Leu Gly Leu Thr Ser Pro Ala Leu Gly Thr Val Ala Val Phe 225 230 235 240 Gly Arg Met Ser Asp Ser Ser Tyr Val His Arg Val Leu Pro Gly Ile                 245 250 255 Thr Gly Gln Asp Gly Met Lys Ser Tyr Ala Ala Gly Val Gln Leu Glu             260 265 270 Arg Ser Val Ala Asn Arg Leu His Phe Asn Gly Ser Val Asn Tyr Thr         275 280 285 Glu Val Asp Pro Lys Leu Ala Ser Thr Lys Gly Phe Lys Gly Val Gly     290 295 300 Phe Asn Val Ser Gly Asp Tyr Ala Gly Asp Gln Tyr Ser Leu Gln Leu 305 310 315 320 Leu Ala Ser Arg Ser Pro Gln Pro Ser Leu Leu Leu Phe Val Gly Tyr                 325 330 335 Glu Ile Val Thr Ala Val Ser Ala Asn Ala Thr Arg Arg Leu Ser Asp             340 345 350 Arg Ile Gln Ile Ser Leu Gln Gly Ser Arg Thr Trp Arg Glu Leu Ala         355 360 365 Ser Ser Arg Leu Leu Thr Asn Val Pro Ile Ser Gly Asn Asp Asn Thr     370 375 380 Ser Thr Leu Phe Ala Ser Ala Thr Phe Arg Pro Asn Arg Arg Leu Ser 385 390 395 400 Phe Val Leu Gly Ala Gly Leu Gln Arg Arg Thr Ser Asn Thr Gln Leu                 405 410 415 Tyr Ser Tyr Ser Ser Lys Arg Ile Asn Leu Ser Thr Ser Leu Ser Leu             420 425 430 <210> 20 <211> 918 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (918) <223> dpsD <400> 20 atg cat atc aag aat cgc ttc gtg aat atc tcg acg ttg gcc atc gcc 48 Met His Ile Lys Asn Arg Phe Val Asn Ile Ser Thr Leu Ala Ile Ala  1 5 10 15   gcc gcg ctg gcc acg ccg gcg gcg gcg cag atc ccc acg cgg tcc gtg 96 Ala Ala Leu Ala Thr Pro Ala Ala Ala Gln Ile Pro Thr Arg Ser Val              20 25 30   ccc gcg ccg gcc cgc ccg cgg cct gca acg ccg ccg gcg caa cag cag 144 Pro Ala Pro Ala Arg Pro Arg Pro Ala Thr Pro Pro Ala Gln Gln Gln          35 40 45   aac cag gcg ccg tcg acg ccc gca gcg gca acc ccg gcg cag acc gcc 192 Asn Gln Ala Pro Ser Thr Pro Ala Ala Ala Thr Pro Ala Gln Thr Ala      50 55 60   gca acc gtt gcc cct gca gca acc gca ccc gca ggt tac aaa atc ggc 240 Ala Thr Val Ala Pro Ala Ala Thr Ala Pro Ala Gly Tyr Lys Ile Gly  65 70 75 80   gtg gac gac gtg atc gag gcc gac gtg ctc ggc cag acc gac ttc aag 288 Val Asp Asp Val Ile Glu Ala Asp Val Leu Gly Gln Thr Asp Phe Lys                  85 90 95   acg cgc gcc cgt gtg cag gcg gac ggc acg gtg acc ctg ccc tat ctg 336 Thr Arg Ala Arg Val Gln Ala Asp Gly Thr Val Thr Leu Pro Tyr Leu             100 105 110   ggc gcc gtg cag gtc aag ggc gag acc gcg acc tcg ctc gcc gaa aag 384 Gly Ala Val Gln Val Lys Gly Glu Thr Ala Thr Ser Leu Ala Glu Lys         115 120 125   ctg gcc ggg ctg ctg cgc gcc ggc ggc tat tat gcc aag ccg atc gtc 432 Leu Ala Gly Leu Leu Arg Ala Gly Gly Tyr Tyr Ala Lys Pro Ile Val     130 135 140   agc gtc gaa atc gtc ggt ttc gtc agc aac tat gtg acg gtg ctg ggc 480 Ser Val Glu Ile Val Gly Phe Val Ser Asn Tyr Val Thr Val Leu Gly 145 150 155 160   cag gtg aac agt tcc ggc ctg cag ccg gtc gac cgc ggc tat cac gtt 528 Gln Val Asn Ser Ser Gly Leu Gln Pro Val Asp Arg Gly Tyr His Val                 165 170 175   tcc gag atc atc gcc cgt gcc ggc ggc ctg cgc ccc gaa gcg gcc gat 576 Ser Glu Ile Ile Ala Arg Ala Gly Gly Leu Arg Pro Glu Ala Ala Asp             180 185 190   ttc gtc gtt ctc acc cgc gcc gat ggc tcc agc gcc aag ctg gac tac 624 Phe Val Val Leu Thr Arg Ala Asp Gly Ser Ser Ala Lys Leu Asp Tyr         195 200 205   aag aag ctc gcc caa ggt ggc ccc aat gac gat ccg atg gtg acg ccc 672 Lys Lys Leu Ala Gln Gly Gly Pro Asn Asp Asp Pro Met Val Thr Pro     210 215 220   ggg gac aag gtc ttt gtc ccg gaa gtc gag cat ttc tac att tat ggt 720 Gly Asp Lys Val Phe Val Pro Glu Val Glu His Phe Tyr Ile Tyr Gly 225 230 235 240   caa att aac gcg cct ggc gta tac gcg att cga tcg gac atg acg ctc 768 Gln Ile Asn Ala Pro Gly Val Tyr Ala Ile Arg Ser Asp Met Thr Leu                 245 250 255   cgt cgc gcg ctg gcc cag ggc ggt ggg ctt gcc ccc gca ggc tcc gtc 816 Arg Arg Ala Leu Ala Gln Gly Gly Gly Leu Ala Pro Ala Gly Ser Val             260 265 270   aag cgt gtg aag gtc acg cgg gat ggc aat gaa ctc aag ttg aag ctg 864 Lys Arg Val Lys Val Thr Arg Asp Gly Asn Glu Leu Lys Leu Lys Leu         275 280 285   gac gat ccg att ctc cca ggc gac acg atc gtc atc ggc gaa cga ttg 912 Asp Asp Pro Ile Leu Pro Gly Asp Thr Ile Val Ile Gly Glu Arg Leu     290 295 300   ttc tga 918 Phe * 305   <210> 21 <211> 305 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative polysaccharide export protein <400> 21 Met His Ile Lys Asn Arg Phe Val Asn Ile Ser Thr Leu Ala Ile Ala  1 5 10 15 Ala Ala Leu Ala Thr Pro Ala Ala Ala Gln Ile Pro Thr Arg Ser Val             20 25 30 Pro Ala Pro Ala Arg Pro Arg Pro Ala Thr Pro Pro Ala Gln Gln Gln         35 40 45 Asn Gln Ala Pro Ser Thr Pro Ala Ala Ala Thr Pro Ala Gln Thr Ala     50 55 60 Ala Thr Val Ala Pro Ala Ala Thr Ala Pro Ala Gly Tyr Lys Ile Gly 65 70 75 80 Val Asp Asp Val Ile Glu Ala Asp Val Leu Gly Gln Thr Asp Phe Lys                 85 90 95 Thr Arg Ala Arg Val Gln Ala Asp Gly Thr Val Thr Leu Pro Tyr Leu             100 105 110 Gly Ala Val Gln Val Lys Gly Glu Thr Ala Thr Ser Leu Ala Glu Lys         115 120 125 Leu Ala Gly Leu Leu Arg Ala Gly Gly Tyr Tyr Ala Lys Pro Ile Val     130 135 140 Ser Val Glu Ile Val Gly Phe Val Ser Asn Tyr Val Thr Val Leu Gly 145 150 155 160 Gln Val Asn Ser Ser Gly Leu Gln Pro Val Asp Arg Gly Tyr His Val                 165 170 175 Ser Glu Ile Ile Ala Arg Ala Gly Gly Leu Arg Pro Glu Ala Ala Asp             180 185 190 Phe Val Val Leu Thr Arg Ala Asp Gly Ser Ser Ala Lys Leu Asp Tyr         195 200 205 Lys Lys Leu Ala Gln Gly Gly Pro Asn Asp Asp Pro Met Val Thr Pro     210 215 220 Gly Asp Lys Val Phe Val Pro Glu Val Glu His Phe Tyr Ile Tyr Gly 225 230 235 240 Gln Ile Asn Ala Pro Gly Val Tyr Ala Ile Arg Ser Asp Met Thr Leu                 245 250 255 Arg Arg Ala Leu Ala Gln Gly Gly Gly Leu Ala Pro Ala Gly Ser Val             260 265 270 Lys Arg Val Lys Val Thr Arg Asp Gly Asn Glu Leu Lys Leu Lys Leu         275 280 285 Asp Asp Pro Ile Leu Pro Gly Asp Thr Ile Val Ile Gly Glu Arg Leu     290 295 300 Phe 305 <210> 22 <211> 1347 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (1347) <223> dpsC <400> 22 gtg aat atc att cag ttc ttc cgc att ctg tgg gtg cgc cga tgg atc 48 Val Asn Ile Ile Gln Phe Phe Arg Ile Leu Trp Val Arg Arg Trp Ile  1 5 10 15   atc ctc ccg gcg ttt ctc gtt tgc gtt acc act gcc acc att gtg gtc 96 Ile Leu Pro Ala Phe Leu Val Cys Val Thr Thr Ala Thr Ile Val Val              20 25 30   cag ttt ctg ccc gaa cgc tac aag gcc act acg cgg gtg gtg ctc gac 144 Gln Phe Leu Pro Glu Arg Tyr Lys Ala Thr Thr Arg Val Val Leu Asp          35 40 45   acg ttt aag ccc gat ccc gtc acc gga cag gtg atg agc tcg cag ttc 192 Thr Phe Lys Pro Asp Pro Val Thr Gly Gln Val Met Ser Ser Gln Phe      50 55 60   atg cgc gcc tat gtc gag act cag acc cag ctg atc gag gac tat gcg 240 Met Arg Ala Tyr Val Glu Thr Gln Thr Gln Leu Ile Glu Asp Tyr Ala  65 70 75 80   acc gcc ggt cgc gtg gtc gac gaa ctg ggc tgg gtg aat gat ccg gcg 288 Thr Ala Gly Arg Val Val Asp Glu Leu Gly Trp Val Asn Asp Pro Ala                  85 90 95   aac atc tcc gcg ttc aac aac tcg tcc gcg gct gcc acc ggc gac atc 336 Asn Ile Ser Ala Phe Asn Asn Ser Ser Ala Ala Ala Thr Gly Asp Ile             100 105 110   cgc cgc tgg ctc gcc aag cag atc atc gac aat acc aag gcc gat gtg 384 Arg Arg Trp Leu Ala Lys Gln Ile Ile Asp Asn Thr Lys Ala Asp Val         115 120 125   atg gag ggg agc aac atc ctc gaa atc acc tat tcg gac agc tcg ccc 432 Met Glu Gly Ser Asn Ile Leu Glu Ile Thr Tyr Ser Asp Ser Ser Pro     130 135 140   gag cgc gcc gaa cgc atc gcc aac ctg atc cgc acc tcg ttc ctc gcc 480 Glu Arg Ala Glu Arg Ile Ala Asn Leu Ile Arg Thr Ser Phe Leu Ala 145 150 155 160   cag tcg ctc gcc gcc aag cgc cag gcc gcg acc aag tcg gcc gac tgg 528 Gln Ser Leu Ala Ala Lys Arg Gln Ala Ala Thr Lys Ser Ala Asp Trp                 165 170 175   tac gcc cag cag gcc gaa gct gcc cgc gat tcg ctc gct gcg gcg gtc 576 Tyr Ala Gln Gln Ala Glu Ala Ala Arg Asp Ser Leu Ala Ala Ala Val             180 185 190   cag gcc cgc acc gat ttc gtg aag aag acc ggc atc gtg ctg acc gaa 624 Gln Ala Arg Thr Asp Phe Val Lys Lys Thr Gly Ile Val Leu Thr Glu         195 200 205   acc ggc gcc gac ctg gaa acc cag aag ctc cag cag atc gag ggg cag 672 Thr Gly Ala Asp Leu Glu Thr Gln Lys Leu Gln Gln Ile Glu Gly Gln     210 215 220   acg acg acc gcc acc gcc ccg gtt gcc atg gcc ccc agc ggc atg ggc 720 Thr Thr Thr Ala Thr Ala Pro Val Ala Met Ala Pro Ser Gly Met Gly 225 230 235 240   ccg gcg cag atg cag ctc gcc cag atc gac cag cag atc cag cag gca 768 Pro Ala Gln Met Gln Leu Ala Gln Ile Asp Gln Gln Ile Gln Gln Ala                 245 250 255   gcg acc agc cta ggt ccg aac cac cca act ttc cag gcc ttg cag cgg 816 Ala Thr Ser Leu Gly Pro Asn His Pro Thr Phe Gln Ala Leu Gln Arg             260 265 270   cag cgc gaa gtg ttc gcc aag gca gcg gcg gcg gaa cgc gcg cag gcg 864 Gln Arg Glu Val Phe Ala Lys Ala Ala Ala Ala Glu Arg Ala Gln Ala         275 280 285   aac ggc gta tcc ggt ccg gca cgc ggg gcc atc gaa agc gca gcc aac 912 Asn Gly Val Ser Gly Pro Ala Arg Gly Ala Ile Glu Ser Ala Ala Asn     290 295 300   gcc cag cgc gcg cgg gtt ctc ggc aat cgt cag gat gtc gac aag ctt 960 Ala Gln Arg Ala Arg Val Leu Gly Asn Arg Gln Asp Val Asp Lys Leu 305 310 315 320   acg cag ctg cag cgt gac gtc tcg ctg aag cag gat cag tac atg aag 1008 Thr Gln Leu Gln Arg Asp Val Ser Leu Lys Gln Asp Gln Tyr Met Lys                 325 330 335   gcg gca cag cgc gtc gcc gat ctg cgg ctg gaa gca agc agc aac gat 1056 Ala Ala Gln Arg Val Ala Asp Leu Arg Leu Glu Ala Ser Ser Asn Asp             340 345 350   gtc ggc atg tcg acg ctc agc gaa gca tcg gcg ccg gaa acg ccc tat 1104 Val Gly Met Ser Thr Leu Ser Glu Ala Ser Ala Pro Glu Thr Pro Tyr         355 360 365   tac ccc aag gtg ccg ctc atc atc ggt ggt gca gcc ggc ttc ggc ctc 1152 Tyr Pro Lys Val Pro Leu Ile Ile Gly Gly Ala Ala Gly Phe Gly Leu     370 375 380   ggg ctc ggt ctg ctg gtc gcg ctg ctc gtc gag ctg ctc ggc cgc cgc 1200 Gly Leu Gly Leu Leu Val Ala Leu Leu Val Glu Leu Leu Gly Arg Arg 385 390 395 400   gtc cgc agc ccc gag gat ctg gaa gtt gcg atc gat gca ccg gtg ctg 1248 Val Arg Ser Pro Glu Asp Leu Glu Val Ala Ile Asp Ala Pro Val Leu                 405 410 415   ggc gtg atc cag agc cgc gcc tcg ctt gcc gcc cgc ctt cgc cgc gcc 1296 Gly Val Ile Gln Ser Arg Ala Ser Leu Ala Ala Arg Leu Arg Arg Ala             420 425 430   caa gaa acc ctc ggc gaa ggt gcc gac acg cac gga gct tca gta aac 1344 Gln Glu Thr Leu Gly Glu Gly Ala Asp Thr His Gly Ala Ser Val Asn         435 440 445   tga 1347  *   <210> 23 <211> 448 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative polysaccharide export protein <400> 23 Val Asn Ile Ile Gln Phe Phe Arg Ile Leu Trp Val Arg Arg Trp Ile  1 5 10 15 Ile Leu Pro Ala Phe Leu Val Cys Val Thr Thr Ala Thr Ile Val Val             20 25 30 Gln Phe Leu Pro Glu Arg Tyr Lys Ala Thr Thr Arg Val Val Leu Asp         35 40 45 Thr Phe Lys Pro Asp Pro Val Thr Gly Gln Val Met Ser Ser Gln Phe     50 55 60 Met Arg Ala Tyr Val Glu Thr Gln Thr Gln Leu Ile Glu Asp Tyr Ala 65 70 75 80 Thr Ala Gly Arg Val Val Asp Glu Leu Gly Trp Val Asn Asp Pro Ala                 85 90 95 Asn Ile Ser Ala Phe Asn Asn Ser Ser Ala Ala Ala Thr Gly Asp Ile             100 105 110 Arg Arg Trp Leu Ala Lys Gln Ile Ile Asp Asn Thr Lys Ala Asp Val         115 120 125 Met Glu Gly Ser Asn Ile Leu Glu Ile Thr Tyr Ser Asp Ser Ser Pro     130 135 140 Glu Arg Ala Glu Arg Ile Ala Asn Leu Ile Arg Thr Ser Phe Leu Ala 145 150 155 160 Gln Ser Leu Ala Ala Lys Arg Gln Ala Ala Thr Lys Ser Ala Asp Trp                 165 170 175 Tyr Ala Gln Gln Ala Glu Ala Ala Arg Asp Ser Leu Ala Ala Ala Val             180 185 190 Gln Ala Arg Thr Asp Phe Val Lys Lys Thr Gly Ile Val Leu Thr Glu         195 200 205 Thr Gly Ala Asp Leu Glu Thr Gln Lys Leu Gln Gln Ile Glu Gly Gln     210 215 220 Thr Thr Thr Ala Thr Ala Pro Val Ala Met Ala Pro Ser Gly Met Gly 225 230 235 240 Pro Ala Gln Met Gln Leu Ala Gln Ile Asp Gln Gln Ile Gln Gln Ala                 245 250 255 Ala Thr Ser Leu Gly Pro Asn His Pro Thr Phe Gln Ala Leu Gln Arg             260 265 270 Gln Arg Glu Val Phe Ala Lys Ala Ala Ala Ala Glu Arg Ala Gln Ala         275 280 285 Asn Gly Val Ser Gly Pro Ala Arg Gly Ala Ile Glu Ser Ala Ala Asn     290 295 300 Ala Gln Arg Ala Arg Val Leu Gly Asn Arg Gln Asp Val Asp Lys Leu 305 310 315 320 Thr Gln Leu Gln Arg Asp Val Ser Leu Lys Gln Asp Gln Tyr Met Lys                 325 330 335 Ala Ala Gln Arg Val Ala Asp Leu Arg Leu Glu Ala Ser Ser Asn Asp             340 345 350 Val Gly Met Ser Thr Leu Ser Glu Ala Ser Ala Pro Glu Thr Pro Tyr         355 360 365 Tyr Pro Lys Val Pro Leu Ile Ile Gly Gly Ala Ala Gly Phe Gly Leu     370 375 380 Gly Leu Gly Leu Leu Val Ala Leu Leu Val Glu Leu Leu Gly Arg Arg 385 390 395 400 Val Arg Ser Pro Glu Asp Leu Glu Val Ala Ile Asp Ala Pro Val Leu                 405 410 415 Gly Val Ile Gln Ser Arg Ala Ser Leu Ala Ala Arg Leu Arg Arg Ala             420 425 430 Gln Glu Thr Leu Gly Glu Gly Ala Asp Thr His Gly Ala Ser Val Asn         435 440 445 <210> 24 <211> 708 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS <222> (1) ... (708) <223> dpsE <400> 24 atg gac gcg atg acc agc gaa ccg ctg ccc gaa ggc gat cgt ccg agc 48 Met Asp Ala Met Thr Ser Glu Pro Leu Pro Glu Gly Asp Arg Pro Ser  1 5 10 15   gcc gtg ccg acc acg ccg gat acg atc ggc atg ctc gaa tac cag ctc 96 Ala Val Pro Thr Thr Pro Asp Thr Ile Gly Met Leu Glu Tyr Gln Leu              20 25 30   gtc ctc tcc gat ccg acc ggg atc gag gcg gaa gcg atc cgc gcg cta 144 Val Leu Ser Asp Pro Thr Gly Ile Glu Ala Glu Ala Ile Arg Ala Leu          35 40 45   cgc acg cgc atc atg acc cag cac ctc cgc gag ggc cgg cgc gcg ctc 192 Arg Thr Arg Ile Met Thr Gln His Leu Arg Glu Gly Arg Arg Ala Leu      50 55 60   gcg atc tgc gcc gcc tcg gcg gga tcc ggc tgc agc ttc acc gcc gtc 240 Ala Ile Cys Ala Ala Ser Ala Gly Ser Gly Cys Ser Phe Thr Ala Val  65 70 75 80   aat ctg gcg acg gcg ctg gcg cag atc ggc gtt aag act gcg ctg gtc 288 Asn Leu Ala Thr Ala Leu Ala Gln Ile Gly Val Lys Thr Ala Leu Val                  85 90 95   gat gcc aat ctg cgc gat ccc agc atc ggc gca gcc ttc ggc ctc gcc 336 Asp Ala Asn Leu Arg Asp Pro Ser Ile Gly Ala Ala Phe Gly Leu Ala             100 105 110   gcc gac aag ccc ggc ctg gcc gat tat ctc gcc tcg ggc gat gtc gac 384 Ala Asp Lys Pro Gly Leu Ala Asp Tyr Leu Ala Ser Gly Asp Val Asp         115 120 125   ctc gcc tcg atc atc cat gcg acc cgc ctc gac cag ctc tcg atc atc 432 Leu Ala Ser Ile Ile His Ala Thr Arg Leu Asp Gln Leu Ser Ile Ile     130 135 140   ccg gcc ggg cat gtc gag cac agc ccg cag gaa ctg ctc gcg tcc gaa 480 Pro Ala Gly His Val Glu His Ser Pro Gln Glu Leu Leu Ala Ser Glu 145 150 155 160   cag ttc cat gat ctg gcg acg cag ctg ctg cgc gag ttc gac atc acg 528 Gln Phe His Asp Leu Ala Thr Gln Leu Leu Arg Glu Phe Asp Ile Thr                 165 170 175   atc ttc gac acc acg gcg tcc aac acc tgc gcc gac gcg cag cgt gtc 576 Ile Phe Asp Thr Thr Ala Ser Asn Thr Cys Ala Asp Ala Gln Arg Val             180 185 190   gcg cat atc gcc ggc tat gcg atc atc gtg gcg cgc aag gat gcg agc 624 Ala His Ile Ala Gly Tyr Ala Ile Ile Val Ala Arg Lys Asp Ala Ser         195 200 205   tac atc cgc gac gtg aac acg ctc agc cgc acg ctg cgt gca gac cgc 672 Tyr Ile Arg Asp Val Asn Thr Leu Ser Arg Thr Leu Arg Ala Asp Arg     210 215 220   acc aac gtc atc ggc tgc gta ctg aac ggc tat tga 708 Thr Asn Val Ile Gly Cys Val Leu Asn Gly Tyr * 225 230 235   <210> 25 <211> 235 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative polysaccharide export protein <400> 25 Met Asp Ala Met Thr Ser Glu Pro Leu Pro Glu Gly Asp Arg Pro Ser  1 5 10 15 Ala Val Pro Thr Thr Pro Asp Thr Ile Gly Met Leu Glu Tyr Gln Leu             20 25 30 Val Leu Ser Asp Pro Thr Gly Ile Glu Ala Glu Ala Ile Arg Ala Leu         35 40 45 Arg Thr Arg Ile Met Thr Gln His Leu Arg Glu Gly Arg Arg Ala Leu     50 55 60 Ala Ile Cys Ala Ala Ser Ala Gly Ser Gly Cys Ser Phe Thr Ala Val 65 70 75 80 Asn Leu Ala Thr Ala Leu Ala Gln Ile Gly Val Lys Thr Ala Leu Val                 85 90 95 Asp Ala Asn Leu Arg Asp Pro Ser Ile Gly Ala Ala Phe Gly Leu Ala             100 105 110 Ala Asp Lys Pro Gly Leu Ala Asp Tyr Leu Ala Ser Gly Asp Val Asp         115 120 125 Leu Ala Ser Ile Ile His Ala Thr Arg Leu Asp Gln Leu Ser Ile Ile     130 135 140 Pro Ala Gly His Val Glu His Ser Pro Gln Glu Leu Leu Ala Ser Glu 145 150 155 160 Gln Phe His Asp Leu Ala Thr Gln Leu Leu Arg Glu Phe Asp Ile Thr                 165 170 175 Ile Phe Asp Thr Thr Ala Ser Asn Thr Cys Ala Asp Ala Gln Arg Val             180 185 190 Ala His Ile Ala Gly Tyr Ala Ile Ile Val Ala Arg Lys Asp Ala Ser         195 200 205 Tyr Ile Arg Asp Val Asn Thr Leu Ser Arg Thr Leu Arg Ala Asp Arg     210 215 220 Thr Asn Val Ile Gly Cys Val Leu Asn Gly Tyr 225 230 235 <210> 26 <211> 882 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS <222> (1) ... (882) <223> dpsM <400> 26 atg gca gcg acc gcg atg acg cgg cag cag gag agg aag ggc ggt ggc 48 Met Ala Ala Thr Ala Met Thr Arg Gln Gln Glu Arg Lys Gly Gly Gly  1 5 10 15   tat tgg ctg gcc gtt gcc ggt ctt gcc gcg cta acc atc ccg acc ttc 96 Tyr Trp Leu Ala Val Ala Gly Leu Ala Ala Leu Thr Ile Pro Thr Phe              20 25 30   atc acc ctg ggt cgc gag gtt tgg agt gcg gaa ggc ggc gtg cag ggt 144 Ile Thr Leu Gly Arg Glu Val Trp Ser Ala Glu Gly Gly Val Gln Gly          35 40 45   ccg atc gtg ctc gcc acg ggc gcc tgg atg ctg gcc cgc cag tgc tcg 192 Pro Ile Val Leu Ala Thr Gly Ala Trp Met Leu Ala Arg Gln Cys Ser      50 55 60   acg atc gag gcg cta cgc cgc ccc ggc agc gtg ctg ctc ggc gcg ctg 240 Thr Ile Glu Ala Leu Arg Arg Pro Gly Ser Val Leu Leu Gly Ala Leu  65 70 75 80   ttc ctg ctg gcg acg ctt gcc ttc tac acc gtt gga cgg gtg ttc gac 288 Phe Leu Leu Ala Thr Leu Ala Phe Tyr Thr Val Gly Arg Val Phe Asp                  85 90 95   ttc atc agt gtc gaa acc ttc gga ctg gtc gcg acc tat ctg gtc gtc 336 Phe Ile Ser Val Glu Thr Phe Gly Leu Val Ala Thr Tyr Leu Val Val             100 105 110   gcc tat ctc tat ttc ggt gcc agg gtg ctc cgt gcc gcc tgg ttc ccg 384 Ala Tyr Leu Tyr Phe Gly Ala Arg Val Leu Arg Ala Ala Trp Phe Pro         115 120 125   gtg ctg tgg ctg ttc ttc ctg gtg ccg ccg ccc ggc tgg gcc gtc gac 432 Val Leu Trp Leu Phe Phe Leu Val Pro Pro Gly Trp Ala Val Asp     130 135 140   cgc atc acc gca ccg ctc aag gag ttc gtc tcc tat gcg gca acg ggc 480 Arg Ile Thr Ala Pro Leu Lys Glu Phe Val Ser Tyr Ala Ala Thr Gly 145 150 155 160   ctg ctt tcc tgg gtg gat tat ccg atc ctg cgc cag ggc gtg aca ctg 528 Leu Leu Ser Trp Val Asp Tyr Pro Ile Leu Arg Gln Gly Val Thr Leu                 165 170 175   ttc gtc ggc ccc tat cag ctg ctc gtc gaa gat gcc tgt tcg ggt ctg 576 Phe Val Gly Pro Tyr Gln Leu Leu Val Glu Asp Ala Cys Ser Gly Leu             180 185 190   cgc tcg ctg tcc agc ctg gtc gtc gtg acg ctg ctc tac atc tac atc 624 Arg Ser Leu Ser Ser Leu Val Val Val Thr Leu Leu Tyr Ile Tyr Ile         195 200 205   aag aac aag ccg tcc tgg cgc tac gcg gcg ttc atc gca gcg ctg gtg 672 Lys Asn Lys Pro Ser Trp Arg Tyr Ala Ala Phe Ile Ala Ala Leu Val     210 215 220   atc ccg gtg gca gtg gtg acc aac gtc ctg cgg atc atc atc ctg gta 720 Ile Pro Val Ala Val Val Thr Asn Val Leu Arg Ile Ile Ile Leu Val 225 230 235 240   ctg atc acc tat cat ctg ggc gac gag gcg gcg cag agc ttc ctc cac 768 Leu Ile Thr Tyr His Leu Gly Asp Glu Ala Ala Gln Ser Phe Leu His                 245 250 255   gtc tcc acc ggc atg gtg atg ttc gtg gtc gcc ctg ctt tgc atc ttc 816 Val Ser Thr Gly Met Val Met Phe Val Val Ala Leu Leu Cys Ile Phe             260 265 270   gcg atc gac tgg gtg gtc gag caa ctt ctt ctc ctg cgt cgg agg cat 864 Ala Ile Asp Trp Val Val Glu Gln Leu Leu Leu Leu Arg Arg Arg His         275 280 285   cat gtt caa ccg gcg tga 882 His Val Gln Pro Ala *     290   <210> 27 <211> 293 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative polysaccharide export protein <400> 27 Met Ala Ala Thr Ala Met Thr Arg Gln Gln Glu Arg Lys Gly Gly Gly  1 5 10 15 Tyr Trp Leu Ala Val Ala Gly Leu Ala Ala Leu Thr Ile Pro Thr Phe             20 25 30 Ile Thr Leu Gly Arg Glu Val Trp Ser Ala Glu Gly Gly Val Gln Gly         35 40 45 Pro Ile Val Leu Ala Thr Gly Ala Trp Met Leu Ala Arg Gln Cys Ser     50 55 60 Thr Ile Glu Ala Leu Arg Arg Pro Gly Ser Val Leu Leu Gly Ala Leu 65 70 75 80 Phe Leu Leu Ala Thr Leu Ala Phe Tyr Thr Val Gly Arg Val Phe Asp                 85 90 95 Phe Ile Ser Val Glu Thr Phe Gly Leu Val Ala Thr Tyr Leu Val Val             100 105 110 Ala Tyr Leu Tyr Phe Gly Ala Arg Val Leu Arg Ala Ala Trp Phe Pro         115 120 125 Val Leu Trp Leu Phe Phe Leu Val Pro Pro Gly Trp Ala Val Asp     130 135 140 Arg Ile Thr Ala Pro Leu Lys Glu Phe Val Ser Tyr Ala Ala Thr Gly 145 150 155 160 Leu Leu Ser Trp Val Asp Tyr Pro Ile Leu Arg Gln Gly Val Thr Leu                 165 170 175 Phe Val Gly Pro Tyr Gln Leu Leu Val Glu Asp Ala Cys Ser Gly Leu             180 185 190 Arg Ser Leu Ser Ser Leu Val Val Val Thr Leu Leu Tyr Ile Tyr Ile         195 200 205 Lys Asn Lys Pro Ser Trp Arg Tyr Ala Ala Phe Ile Ala Ala Leu Val     210 215 220 Ile Pro Val Ala Val Val Thr Asn Val Leu Arg Ile Ile Ile Leu Val 225 230 235 240 Leu Ile Thr Tyr His Leu Gly Asp Glu Ala Ala Gln Ser Phe Leu His                 245 250 255 Val Ser Thr Gly Met Val Met Phe Val Val Ala Leu Leu Cys Ile Phe             260 265 270 Ala Ile Asp Trp Val Val Glu Gln Leu Leu Leu Leu Arg Arg Arg His         275 280 285 His Val Gln Pro Ala     290 <210> 28 <211> 699 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS <222> (1) ... (699) <223> dpsN <400> 28 atg ttc aac cgg cgt gac ctg ctg atc ggc gca ggc tgc ttc gcc gcc 48 Met Phe Asn Arg Arg Asp Leu Leu Ile Gly Ala Gly Cys Phe Ala Ala  1 5 10 15   gct ggc gcc tcg ctc ggc ctg aag ccg cac cgg cgg atg gac ctg ctg 96 Ala Gly Ala Ser Leu Gly Leu Lys Pro His Arg Arg Met Asp Leu Leu              20 25 30   ggc ggc acc aag ctc gac acg ctg atg ccc aag gca ttc ggc gca tgg 144 Gly Gly Thr Lys Leu Asp Thr Leu Met Pro Lys Ala Phe Gly Ala Trp          35 40 45   aag gca gag gat acc ggt tcg ctg atc gcg ccg gcg cgc gaa ggc agc 192 Lys Ala Glu Asp Thr Gly Ser Leu Ile Ala Pro Ala Arg Glu Gly Ser      50 55 60   ctg gag gac aag ctc tac aac cag gtg gtc acc cgc gcc ttc tcc cgc 240 Leu Glu Asp Lys Leu Tyr Asn Gln Val Val Thr Arg Ala Phe Ser Arg  65 70 75 80   gcg gac ggt gcc caa gtg atg ctg ctg atc gcc tat ggc aac gcc cag 288 Ala Asp Gly Ala Gln Val Met Leu Leu Ile Ala Tyr Gly Asn Ala Gln                  85 90 95   acc gat cta ctg cag ctg cac cgg ccg gaa ata tgc tac ccg ttc ttc 336 Thr Asp Leu Leu Gln Leu His Arg Pro Glu Ile Cys Tyr Pro Phe Phe             100 105 110   ggc ttc acc gtg gtg gaa agc cat gag cag acc atc ccg gtg acg ccg 384 Gly Phe Thr Val Val Glu Ser His Glu Gln Thr Ile Pro Val Thr Pro         115 120 125   cag gtg acg atc ccc ggt cgc gcg ctg acc gcc acc aac ttc aac cgc 432 Gln Val Thr Ile Pro Gly Arg Ala Leu Thr Ala Thr Asn Phe Asn Arg     130 135 140   acc gag cag atc ctc tac tgg acc cgc gtc ggc gaa tat ctg ccg cag 480 Thr Glu Gln Ile Leu Tyr Trp Thr Arg Val Gly Glu Tyr Leu Pro Gln 145 150 155 160   aac ggc aat cag cag atg ctc gcg cgg ctg aag agc cag gtc cag ggc 528 Asn Gly Asn Gln Gln Met Leu Ala Arg Leu Lys Ser Gln Val Gln Gly                 165 170 175   tgg atc gtc gac ggt gtg ctg gtg cgc atc tcg acg gtg acg ccc gag 576 Trp Ile Val Asp Gly Val Leu Val Arg Ile Ser Thr Val Thr Pro Glu             180 185 190   gcg gaa gat ggc ctg agc gcc aat ctc gat ttc gcg cgc gag ctg gtg 624 Ala Glu Asp Gly Leu Ser Ala Asn Leu Asp Phe Ala Arg Glu Leu Val         195 200 205   aag acg ctc gac ccg cgc gtg ctg cgc ccg ctg ctc ggg aac ggg ctc 672 Lys Thr Leu Asp Pro Arg Val Leu Arg Pro Leu Leu Gly Asn Gly Leu     210 215 220   aca cgg cag ctc ggt cac cag gtc tga 699 Thr Arg Gln Leu Gly His Gln Val * 225 230   <210> 29 <211> 232 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative polysaccharide export protein <400> 29 Met Phe Asn Arg Arg Asp Leu Leu Ile Gly Ala Gly Cys Phe Ala Ala  1 5 10 15 Ala Gly Ala Ser Leu Gly Leu Lys Pro His Arg Arg Met Asp Leu Leu             20 25 30 Gly Gly Thr Lys Leu Asp Thr Leu Met Pro Lys Ala Phe Gly Ala Trp         35 40 45 Lys Ala Glu Asp Thr Gly Ser Leu Ile Ala Pro Ala Arg Glu Gly Ser     50 55 60 Leu Glu Asp Lys Leu Tyr Asn Gln Val Val Thr Arg Ala Phe Ser Arg 65 70 75 80 Ala Asp Gly Ala Gln Val Met Leu Leu Ile Ala Tyr Gly Asn Ala Gln                 85 90 95 Thr Asp Leu Leu Gln Leu His Arg Pro Glu Ile Cys Tyr Pro Phe Phe             100 105 110 Gly Phe Thr Val Val Glu Ser His Glu Gln Thr Ile Pro Val Thr Pro         115 120 125 Gln Val Thr Ile Pro Gly Arg Ala Leu Thr Ala Thr Asn Phe Asn Arg     130 135 140 Thr Glu Gln Ile Leu Tyr Trp Thr Arg Val Gly Glu Tyr Leu Pro Gln 145 150 155 160 Asn Gly Asn Gln Gln Met Leu Ala Arg Leu Lys Ser Gln Val Gln Gly                 165 170 175 Trp Ile Val Asp Gly Val Leu Val Arg Ile Ser Thr Val Thr Pro Glu             180 185 190 Ala Glu Asp Gly Leu Ser Ala Asn Leu Asp Phe Ala Arg Glu Leu Val         195 200 205 Lys Thr Leu Asp Pro Arg Val Leu Arg Pro Leu Leu Gly Asn Gly Leu     210 215 220 Thr Arg Gln Leu Gly His Gln Val 225 230 <210> 30 <211> 1395 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (1395) <223> atrD <400> 30 atg aac gcc gtt gtt ccg atg cgc cgc ggc ggc ccg ctc gcc cgc atg 48 Met Asn Ala Val Val Pro Met Arg Arg Gly Gly Pro Leu Ala Arg Met  1 5 10 15   cgc gat acc gtg ctg cct gcc cgc gtc gac gct tat gac acc gcc ttc 96 Arg Asp Thr Val Leu Pro Ala Arg Val Asp Ala Tyr Asp Thr Ala Phe              20 25 30   ctg cct gcc gcg ctg gag atc atc gag cgg ccg gtt tcg ccc acc gcg 144 Leu Pro Ala Ala Leu Glu Ile Ile Glu Arg Pro Val Ser Pro Thr Ala          35 40 45   cgg ctt acc gcc aag gtg atg ctg gcc ggg ctg gcg atc acc gcc gcc 192 Arg Leu Thr Ala Lys Val Met Leu Ala Gly Leu Ala Ile Thr Ala Ala      50 55 60   tgg ctg gcg atc ggc aag gtc gaa gtc gtc gcg ccg acg cag ggg cgg 240 Trp Leu Ala Ile Gly Lys Val Glu Val Val Ala Pro Thr Gln Gly Arg  65 70 75 80   atc gcg ccg atc ggc gag acc aag atc gtc cag tcg ccc gaa tcg ggg 288 Ile Ala Pro Ile Gly Glu Thr Lys Ile Val Gln Ser Pro Glu Ser Gly                  85 90 95   atc gtc cgc cgc atc ctg gtg ggc gag ggg cag aag gtc gcc aag ggc 336 Ile Val Arg Arg Ile Leu Val Gly Glu Gly Gln Lys Val Ala Lys Gly             100 105 110   cag gtg ctg atc acg ctc gac ccg acc gtg tcg tcg gcg gag gcg gca 384 Gln Val Leu Ile Thr Leu Asp Pro Thr Val Ser Ser Ala Glu Ala Ala         115 120 125   cag gcg aag gtg gcg ctg ctc agc gcc cag ctc gac gcc gca cgc aac 432 Gln Ala Lys Val Ala Leu Leu Ser Ala Gln Leu Asp Ala Ala Arg Asn     130 135 140   cag gcg atc atc gac gcg ctg gac ggc agg ggc ttc cgc ttc gtc gcg 480 Gln Ala Ile Ile Asp Ala Leu Asp Gly Arg Gly Phe Arg Phe Val Ala 145 150 155 160   cct gcc gcc gcc agc ccg ggc gaa gtg gcg acg cat cgc ggc ctc gcc 528 Pro Ala Ala Ala Ser Pro Gly Glu Val Ala Thr His Arg Gly Leu Ala                 165 170 175   cgc gcc cgg ctg ggc cag atc gag gcg gcg ctg gcc ggc ggc cgc tcc 576 Arg Ala Arg Leu Gly Gln Ile Glu Ala Ala Leu Ala Gly Gly Arg Ser             180 185 190   gat cgc ggt gcc gcc gtc tcg gcc gcg gcc gag gcg cag gca cag gtg 624 Asp Arg Gly Ala Ala Val Ser Ala Ala Ala Glu Ala Gln Ala Gln Val         195 200 205   cgg aag ctc gaa cag tcg ctg ccg ctg ctc gaa cag cag atc gcc gcg 672 Arg Lys Leu Glu Gln Ser Leu Pro Leu Leu Glu Gln Gln Ile Ala Ala     210 215 220   aac gag acg atg gcc gcc aag ggc tat gtc tcg aag ctg cgc gtc gtg 720 Asn Glu Thr Met Ala Ala Lys Gly Tyr Val Ser Lys Leu Arg Val Val 225 230 235 240   gag atg cgt cgc cag ctg atc gcc gag cgg cag gac ctg acg gcg gcg 768 Glu Met Arg Arg Gln Leu Ile Ala Glu Arg Gln Asp Leu Thr Ala Ala                 245 250 255   cgc gct acg ctc gcc aaa ctc ggc cag cag tcg ctg agc gtc tcc agc 816 Arg Ala Thr Leu Ala Lys Leu Gly Gln Gln Ser Leu Ser Val Ser Ser             260 265 270   ctg tcg gcc aag acg cgc gag gag gcg cgg gcg cag gtg ctg cag gat 864 Leu Ser Ala Lys Thr Arg Glu Glu Ala Arg Ala Gln Val Leu Gln Asp         275 280 285   ctg gtc aag gcg cag gac gag gtg cgt gcc cgc ggc gag gac gtc gcc 912 Leu Val Lys Ala Gln Asp Glu Val Arg Ala Arg Gly Glu Asp Val Ala     290 295 300   aag gcg aat ctg cgc agc tcg ttc cgc gaa ctg cgc gcg ccg gtg agc 960 Lys Ala Asn Leu Arg Ser Ser Phe Arg Glu Leu Arg Ala Pro Val Ser 305 310 315 320   ggt acc gtc tcg cag ctg cag gtc cac acc gaa ggc ggc gtg gtg gaa 1008 Gly Thr Val Ser Gln Leu Gln Val His Thr Glu Gly Gly Val Val Glu                 325 330 335   ggg gcc aag ccg ctc ctc agc ctg gtt ccc gac aat gcc cgg ctc gag 1056 Gly Ala Lys Pro Leu Leu Ser Leu Val Pro Asp Asn Ala Arg Leu Glu             340 345 350   gcc gag gtg atg gtc gac aac agc gac atc ggc ttc gtc cac atc ggc 1104 Ala Glu Val Met Val Asp Asn Ser Asp Ile Gly Phe Val His Ile Gly         355 360 365   atg ccg gta aag gtg aag ctg cag gcc ttt ccc tat acc cgc tac ggc 1152 Met Pro Val Lys Val Lys Leu Gln Ala Phe Pro Tyr Thr Arg Tyr Gly     370 375 380   atg att ccc ggc acg gtg gcg ggc atc agc ccc gag gcg gtg cag atg 1200 Met Ile Pro Gly Thr Val Ala Gly Ile Ser Pro Glu Ala Val Gln Met 385 390 395 400   aag gag aac cag ccg ccg gtc tac aag gcg cgg atc gcg ctg gcg cgc 1248 Lys Glu Asn Gln Pro Pro Val Tyr Lys Ala Arg Ile Ala Leu Ala Arg                 405 410 415   ggg tat gtg ctg gcc cat ggc gca cag gtg ccg ctg cgg ccg ggg atg 1296 Gly Tyr Val Leu Ala His Gly Ala Gln Val Pro Leu Arg Pro Gly Met             420 425 430   ctc gcg agc gcg gac atc gtc acc ggc aag cga acc ctg ttc agc tat 1344 Leu Ala Ser Ala Asp Ile Val Thr Gly Lys Arg Thr Leu Phe Ser Tyr         435 440 445   ctg gtg ggg ccc gtg ctc gag acg ggg agt gac gcg ctg cac gag cgg 1392 Leu Val Gly Pro Val Leu Glu Thr Gly Ser Asp Ala Leu His Glu Arg     450 455 460   tga 1395  *   <210> 31 <211> 464 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative secretion protein <400> 31 Met Asn Ala Val Val Pro Met Arg Arg Gly Gly Pro Leu Ala Arg Met  1 5 10 15 Arg Asp Thr Val Leu Pro Ala Arg Val Asp Ala Tyr Asp Thr Ala Phe             20 25 30 Leu Pro Ala Ala Leu Glu Ile Ile Glu Arg Pro Val Ser Pro Thr Ala         35 40 45 Arg Leu Thr Ala Lys Val Met Leu Ala Gly Leu Ala Ile Thr Ala Ala     50 55 60 Trp Leu Ala Ile Gly Lys Val Glu Val Val Ala Pro Thr Gln Gly Arg 65 70 75 80 Ile Ala Pro Ile Gly Glu Thr Lys Ile Val Gln Ser Pro Glu Ser Gly                 85 90 95 Ile Val Arg Arg Ile Leu Val Gly Glu Gly Gln Lys Val Ala Lys Gly             100 105 110 Gln Val Leu Ile Thr Leu Asp Pro Thr Val Ser Ser Ala Glu Ala Ala         115 120 125 Gln Ala Lys Val Ala Leu Leu Ser Ala Gln Leu Asp Ala Ala Arg Asn     130 135 140 Gln Ala Ile Ile Asp Ala Leu Asp Gly Arg Gly Phe Arg Phe Val Ala 145 150 155 160 Pro Ala Ala Ala Ser Pro Gly Glu Val Ala Thr His Arg Gly Leu Ala                 165 170 175 Arg Ala Arg Leu Gly Gln Ile Glu Ala Ala Leu Ala Gly Gly Arg Ser             180 185 190 Asp Arg Gly Ala Ala Val Ser Ala Ala Ala Glu Ala Gln Ala Gln Val         195 200 205 Arg Lys Leu Glu Gln Ser Leu Pro Leu Leu Glu Gln Gln Ile Ala Ala     210 215 220 Asn Glu Thr Met Ala Ala Lys Gly Tyr Val Ser Lys Leu Arg Val Val 225 230 235 240 Glu Met Arg Arg Gln Leu Ile Ala Glu Arg Gln Asp Leu Thr Ala Ala                 245 250 255 Arg Ala Thr Leu Ala Lys Leu Gly Gln Gln Ser Leu Ser Val Ser Ser             260 265 270 Leu Ser Ala Lys Thr Arg Glu Glu Ala Arg Ala Gln Val Leu Gln Asp         275 280 285 Leu Val Lys Ala Gln Asp Glu Val Arg Ala Arg Gly Glu Asp Val Ala     290 295 300 Lys Ala Asn Leu Arg Ser Ser Phe Arg Glu Leu Arg Ala Pro Val Ser 305 310 315 320 Gly Thr Val Ser Gln Leu Gln Val His Thr Glu Gly Gly Val Val Glu                 325 330 335 Gly Ala Lys Pro Leu Leu Ser Leu Val Pro Asp Asn Ala Arg Leu Glu             340 345 350 Ala Glu Val Met Val Asp Asn Ser Asp Ile Gly Phe Val His Ile Gly         355 360 365 Met Pro Val Lys Val Lys Leu Gln Ala Phe Pro Tyr Thr Arg Tyr Gly     370 375 380 Met Ile Pro Gly Thr Val Ala Gly Ile Ser Pro Glu Ala Val Gln Met 385 390 395 400 Lys Glu Asn Gln Pro Pro Val Tyr Lys Ala Arg Ile Ala Leu Ala Arg                 405 410 415 Gly Tyr Val Leu Ala His Gly Ala Gln Val Pro Leu Arg Pro Gly Met             420 425 430 Leu Ala Ser Ala Asp Ile Val Thr Gly Lys Arg Thr Leu Phe Ser Tyr         435 440 445 Leu Val Gly Pro Val Leu Glu Thr Gly Ser Asp Ala Leu His Glu Arg     450 455 460 <210> 32 <211> 2187 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (2187) <223> atrB <400> 32 atg aca cgc gac gaa atg cag gcc acc ctg cag agc gcg ctc gcg gcc 48 Met Thr Arg Asp Glu Met Gln Ala Thr Leu Gln Ser Ala Leu Ala Ala  1 5 10 15   cat ggg gcg gcg gag cgc gag gcg gag ctg cgc gaa tcc gga ctg gtg 96 His Gly Ala Ala Glu Arg Glu Ala Glu Leu Arg Glu Ser Gly Leu Val              20 25 30   gcg ttg tcg ctg ctg ctc ggc gcg cac aac atc gcc atc acg ccc gaa 144 Ala Leu Ser Leu Leu Leu Gly Ala His Asn Ile Ala Ile Thr Pro Glu          35 40 45   cag ctg cgc cac gcg ctg ggc cat gcc gag gcg gca agc gcc gac gac 192 Gln Leu Arg His Ala Leu Gly His Ala Glu Ala Ala Ser Ala Asp Asp      50 55 60   ctg atc ctc ctg gcc aag cgc cag cag ggc gtg cgc gcc aag gcc gtc 240 Leu Ile Leu Leu Ala Lys Arg Gln Gln Gly Val Arg Ala Lys Ala Val  65 70 75 80   gag gtg ccg cgc ggc gga ctc gcc cgc cag ccg ctg ccc gcg atc gcc 288 Glu Val Pro Arg Gly Gly Leu Ala Arg Gln Pro Leu Pro Ala Ile Ala                  85 90 95   gac ggg ccc gaa ggc tgg ttc gtg atc ggc ggc ctg acc gaa cat ggc 336 Asp Gly Pro Glu Gly Trp Phe Val Ile Gly Gly Leu Thr Glu His Gly             100 105 110   gtg atc atc cag cgc ccg ggc cat gcc ccg gaa cag gtc gac cgg gac 384 Val Ile Ile Gln Arg Pro Gly His Ala Pro Glu Gln Val Asp Arg Asp         115 120 125   gcg ctg gac gcg atc tgg tcc ggc gcg ctg gtg ctg ctc acc acc cgc 432 Ala Leu Asp Ala Ile Trp Ser Gly Ala Leu Val Leu Leu Thr Thr Arg     130 135 140   gcg gtg gcg gga cgg ccg ctg cgg ttc ggc ctc tcc tgg ttc acc gcg 480 Ala Val Ala Gly Arg Pro Leu Arg Phe Gly Leu Ser Trp Phe Thr Ala 145 150 155 160   cag ttc cgg cgc tat cgc acg ctg ttc ctc gag gtg ctc ggc atc acc 528 Gln Phe Arg Arg Tyr Arg Thr Leu Phe Leu Glu Val Leu Gly Ile Thr                 165 170 175   ctc gcg ctc aac ctg ctc ggc ctc gcc gcg ccg ctg ttg ttc cag agc 576 Leu Ala Leu Asn Leu Leu Gly Leu Ala Ala Pro Leu Leu Phe Gln Ser             180 185 190   gtg atc gac aag gtg ctg atc cac aac agc atg agc acg ctg agc gtg 624 Val Ile Asp Lys Val Leu Ile His Asn Ser Met Ser Thr Leu Ser Val         195 200 205   ctc gcc ttc gcc ttc ctg gcg gtt tcg gtg tgg gaa gtg gcg ctc ggc 672 Leu Ala Phe Ala Phe Leu Ala Val Ser Val Trp Glu Val Ala Leu Gly     210 215 220   tgg atc cgc acc cgc ctg ttc acc gag acg acg cag aag atc gac gtc 720 Trp Ile Arg Thr Arg Leu Phe Thr Glu Thr Thr Gln Lys Ile Asp Val 225 230 235 240   gag ctg ggt gcc cgg ctg ttc cac cac ctg ctg gcg ctg ccg ctc gcc 768 Glu Leu Gly Ala Arg Leu Phe His His Leu Leu Ala Leu Pro Leu Ala                 245 250 255   tat ttc gag aag cgc cgc gtg ggc gac acc gtc acc cgc gtc cgc cag 816 Tyr Phe Glu Lys Arg Arg Val Gly Asp Thr Val Thr Arg Val Arg Gln             260 265 270   ctc gag acg atc cgc gaa ttc ctt acc agc gcc tcg ctg acg gtg atg 864 Leu Glu Thr Ile Arg Glu Phe Leu Thr Ser Ala Ser Leu Thr Val Met         275 280 285   gtg gac ccg ctg ttc acc ttc gtg ttc ctc gcc gcg atg ctg ttc tac 912 Val Asp Pro Leu Phe Thr Phe Val Phe Leu Ala Ala Met Leu Phe Tyr     290 295 300   tcg ccg atg ctc tcg ggc atc gtg ctc gtg tcg ctg atc gcc tat gcg 960 Ser Pro Met Leu Ser Gly Ile Val Leu Val Ser Leu Ile Ala Tyr Ala 305 310 315 320   atc gta tcg ttc agc gtc gcc ggg ccg ctc cgc gcg cgg gtg gag gac 1008 Ile Val Ser Phe Ser Val Ala Gly Pro Leu Arg Ala Arg Val Glu Asp                 325 330 335   aag ttc gag aag agc tcc gcc agc aac gcg ctg ctc gtc gag agc gtc 1056 Lys Phe Glu Lys Ser Ser Ala Ser Asn Ala Leu Leu Val Glu Ser Val             340 345 350   tcg ggc atc cac acg atc aag gcg acc gcg gtc gag ccg cac tgg cag 1104 Ser Gly Ile His Thr Ile Lys Ala Thr Ala Val Glu Pro His Trp Gln         355 360 365   aat cgc tgg gag cgc cag ctc gcc gcc cat acc gcc gcg tcg cag cgg 1152 Asn Arg Trp Glu Arg Gln Leu Ala Ala His Thr Ala Ala Ser Gln Arg     370 375 380   ctg atc aat acc gcc aac acc ggc agc cag gcg atc gag ctg atc tcg 1200 Leu Ile Asn Thr Ala Asn Thr Gly Ser Gln Ala Ile Glu Leu Ile Ser 385 390 395 400   aag ctg agc ttc gcg gcg atc ctg ttc ttc ggc gcc aag gcg gtg atc 1248 Lys Leu Ser Phe Ala Ala Ile Leu Phe Phe Gly Ala Lys Ala Val Ile                 405 410 415   ggc ggc gcg atg agc gta ggc gcg ctg gtg gcg ttc aac atg ttc gcc 1296 Gly Gly Ala Met Ser Val Gly Ala Leu Val Ala Phe Asn Met Phe Ala             420 425 430   cag cgc gtg tcc ggg ccg gtg atc cgc atg gcg cag ctg tgg cag gat 1344 Gln Arg Val Ser Gly Pro Val Ile Arg Met Ala Gln Leu Trp Gln Asp         435 440 445   ttc cag cag gtg cgc atc tcg gtc gag cgg ctg ggc gac gtg ctc aac 1392 Phe Gln Gln Val Arg Ile Ser Val Glu Arg Leu Gly Asp Val Leu Asn     450 455 460   cat ccg gtg gaa ccg cgc ccg gcc tcg gcg gcg acg ctg ccg gtg ctg 1440 His Pro Val Glu Pro Arg Pro Ala Ser Ala Ala Thr Leu Pro Val Leu 465 470 475 480   cgc ggt gcg att cgc ttc gag aat gtc agc ttc cgc tat gcc gag gac 1488 Arg Gly Ala Ile Arg Phe Glu Asn Val Ser Phe Arg Tyr Ala Glu Asp                 485 490 495   cag ccg ccg gtg ctg agc gac atc acg ctc gac att ccg gcg ggc acc 1536 Gln Pro Pro Val Leu Ser Asp Ile Thr Leu Asp Ile Pro Ala Gly Thr             500 505 510   tcg ctc ggc atc gtc ggt tcg tcg ggc tcg ggc aag tcg acg ctg gcc 1584 Ser Leu Gly Ile Val Gly Ser Ser Gly Ser Gly Lys Ser Thr Leu Ala         515 520 525   aag ctg ctc cag cgg ctc aac ctg ccg aat ctc ggc cgc gtg ctg gtc 1632 Lys Leu Leu Gln Arg Leu Asn Leu Pro Asn Leu Gly Arg Val Leu Val     530 535 540   gac gag gtc gac gtg gcg cag ctc gat ccc gcc tgg ctg cgt cgc cag 1680 Asp Glu Val Asp Val Ala Gln Leu Asp Pro Ala Trp Leu Arg Arg Gln 545 550 555 560   atc ggc gtc gtg ctg cag gag aat ctg ctg ttc agc cgc tcg atc cgc 1728 Ile Gly Val Val Leu Gln Glu Asn Leu Leu Phe Ser Arg Ser Ile Arg                 565 570 575   gag aac atc gcg ctc tcc aac ccc gcc atg ccg ttc gag aat gtc gtc 1776 Glu Asn Ile Ala Leu Ser Asn Pro Ala Met Pro Phe Glu Asn Val Val             580 585 590   gcg gcg gcg acg ctg gcc ggc gcg cat gat ttc atc ctg cgc cag ccg 1824 Ala Ala Ala Thr Leu Ala Gly Ala His Asp Phe Ile Leu Arg Gln Pro         595 600 605   cgc ggc tat gac acc gag atc gtc gag cgc ggc gtc aat ctc tcc ggc 1872 Arg Gly Tyr Asp Thr Glu Ile Val Glu Arg Gly Val Asn Leu Ser Gly     610 615 620   ggc cag cgc cag cgg ctc gcc atc gcc cgc gcg ctc gtc ggc aat ccg 1920 Gly Gln Arg Gln Arg Leu Ala Ile Ala Arg Ala Leu Val Gly Asn Pro 625 630 635 640   cgc atc ctg gtg ttc gac gaa gcg acc tcg gcg ctc gat gcc gag agc 1968 Arg Ile Leu Val Phe Asp Glu Ala Thr Ser Ala Leu Asp Ala Glu Ser                 645 650 655   gag gag ctg atc cag aac aac ctg cgc gcc atc tcg gcc ggc cgc acg 2016 Glu Glu Leu Ile Gln Asn Asn Leu Arg Ala Ile Ser Ala Gly Arg Thr             660 665 670   ctc gtg gtg atc gcg cat cgc ctg agc gcg gtg cgc agc tgc gac cgg 2064 Leu Val Val Ile Ala His Arg Leu Ser Ala Val Arg Ser Cys Asp Arg         675 680 685   atc atc acg ctc gaa cag ggc cgc atc gtc gag agc ggc cga cac gac 2112 Ile Ile Thr Leu Glu Gln Gly Arg Ile Val Glu Ser Gly Arg His Asp     690 695 700   gaa ttg ttg cgc ctg ggc ggc cgc tat gcc gac ctg cac cgc cgc cag 2160 Glu Leu Leu Arg Leu Gly Gly Arg Tyr Ala Asp Leu His Arg Arg Gln 705 710 715 720   ggc ggc tat ggg gag att gcc gca tga 2187 Gly Gly Tyr Gly Glu Ile Ala Ala *                 725   <210> 33 <211> 728 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> putative secretion protein <400> 33 Met Thr Arg Asp Glu Met Gln Ala Thr Leu Gln Ser Ala Leu Ala Ala  1 5 10 15 His Gly Ala Ala Glu Arg Glu Ala Glu Leu Arg Glu Ser Gly Leu Val             20 25 30 Ala Leu Ser Leu Leu Leu Gly Ala His Asn Ile Ala Ile Thr Pro Glu         35 40 45 Gln Leu Arg His Ala Leu Gly His Ala Glu Ala Ala Ser Ala Asp Asp     50 55 60 Leu Ile Leu Leu Ala Lys Arg Gln Gln Gly Val Arg Ala Lys Ala Val 65 70 75 80 Glu Val Pro Arg Gly Gly Leu Ala Arg Gln Pro Leu Pro Ala Ile Ala                 85 90 95 Asp Gly Pro Glu Gly Trp Phe Val Ile Gly Gly Leu Thr Glu His Gly             100 105 110 Val Ile Ile Gln Arg Pro Gly His Ala Pro Glu Gln Val Asp Arg Asp         115 120 125 Ala Leu Asp Ala Ile Trp Ser Gly Ala Leu Val Leu Leu Thr Thr Arg     130 135 140 Ala Val Ala Gly Arg Pro Leu Arg Phe Gly Leu Ser Trp Phe Thr Ala 145 150 155 160 Gln Phe Arg Arg Tyr Arg Thr Leu Phe Leu Glu Val Leu Gly Ile Thr                 165 170 175 Leu Ala Leu Asn Leu Leu Gly Leu Ala Ala Pro Leu Leu Phe Gln Ser             180 185 190 Val Ile Asp Lys Val Leu Ile His Asn Ser Met Ser Thr Leu Ser Val         195 200 205 Leu Ala Phe Ala Phe Leu Ala Val Ser Val Trp Glu Val Ala Leu Gly     210 215 220 Trp Ile Arg Thr Arg Leu Phe Thr Glu Thr Thr Gln Lys Ile Asp Val 225 230 235 240 Glu Leu Gly Ala Arg Leu Phe His His Leu Leu Ala Leu Pro Leu Ala                 245 250 255 Tyr Phe Glu Lys Arg Arg Val Gly Asp Thr Val Thr Arg Val Arg Gln             260 265 270 Leu Glu Thr Ile Arg Glu Phe Leu Thr Ser Ala Ser Leu Thr Val Met         275 280 285 Val Asp Pro Leu Phe Thr Phe Val Phe Leu Ala Ala Met Leu Phe Tyr     290 295 300 Ser Pro Met Leu Ser Gly Ile Val Leu Val Ser Leu Ile Ala Tyr Ala 305 310 315 320 Ile Val Ser Phe Ser Val Ala Gly Pro Leu Arg Ala Arg Val Glu Asp                 325 330 335 Lys Phe Glu Lys Ser Ser Ala Ser Asn Ala Leu Leu Val Glu Ser Val             340 345 350 Ser Gly Ile His Thr Ile Lys Ala Thr Ala Val Glu Pro His Trp Gln         355 360 365 Asn Arg Trp Glu Arg Gln Leu Ala Ala His Thr Ala Ala Ser Gln Arg     370 375 380 Leu Ile Asn Thr Ala Asn Thr Gly Ser Gln Ala Ile Glu Leu Ile Ser 385 390 395 400 Lys Leu Ser Phe Ala Ala Ile Leu Phe Phe Gly Ala Lys Ala Val Ile                 405 410 415 Gly Gly Ala Met Ser Val Gly Ala Leu Val Ala Phe Asn Met Phe Ala             420 425 430 Gln Arg Val Ser Gly Pro Val Ile Arg Met Ala Gln Leu Trp Gln Asp         435 440 445 Phe Gln Gln Val Arg Ile Ser Val Glu Arg Leu Gly Asp Val Leu Asn     450 455 460 His Pro Val Glu Pro Arg Pro Ala Ser Ala Ala Thr Leu Pro Val Leu 465 470 475 480 Arg Gly Ala Ile Arg Phe Glu Asn Val Ser Phe Arg Tyr Ala Glu Asp                 485 490 495 Gln Pro Pro Val Leu Ser Asp Ile Thr Leu Asp Ile Pro Ala Gly Thr             500 505 510 Ser Leu Gly Ile Val Gly Ser Ser Gly Ser Gly Lys Ser Thr Leu Ala         515 520 525 Lys Leu Leu Gln Arg Leu Asn Leu Pro Asn Leu Gly Arg Val Leu Val     530 535 540 Asp Glu Val Asp Val Ala Gln Leu Asp Pro Ala Trp Leu Arg Arg Gln 545 550 555 560 Ile Gly Val Val Leu Gln Glu Asn Leu Leu Phe Ser Arg Ser Ile Arg                 565 570 575 Glu Asn Ile Ala Leu Ser Asn Pro Ala Met Pro Phe Glu Asn Val Val             580 585 590 Ala Ala Ala Thr Leu Ala Gly Ala His Asp Phe Ile Leu Arg Gln Pro         595 600 605 Arg Gly Tyr Asp Thr Glu Ile Val Glu Arg Gly Val Asn Leu Ser Gly     610 615 620 Gly Gln Arg Gln Arg Leu Ala Ile Ala Arg Ala Leu Val Gly Asn Pro 625 630 635 640 Arg Ile Leu Val Phe Asp Glu Ala Thr Ser Ala Leu Asp Ala Glu Ser                 645 650 655 Glu Glu Leu Ile Gln Asn Asn Leu Arg Ala Ile Ser Ala Gly Arg Thr             660 665 670 Leu Val Val Ile Ala His Arg Leu Ser Ala Val Arg Ser Cys Asp Arg         675 680 685 Ile Ile Thr Leu Glu Gln Gly Arg Ile Val Glu Ser Gly Arg His Asp     690 695 700 Glu Leu Leu Arg Leu Gly Gly Arg Tyr Ala Asp Leu His Arg Arg Gln 705 710 715 720 Gly Gly Tyr Gly Glu Ile Ala Ala                 725 <210> 34 <211> 1413 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (1413) <223> dpsB <400> 34 atg aac gct ttc gaa gca cag cgc gcc ttt gag gag caa ctt cgg gcg 48 Met Asn Ala Phe Glu Ala Gln Arg Ala Phe Glu Glu Gln Leu Arg Ala  1 5 10 15   cat tcc cgg gtt acg cca tct gcc gct ccc gtg tgg cgt cgc tcg acg 96 His Ser Arg Val Thr Pro Ser Ala Ala Pro Val Trp Arg Arg Ser Thr              20 25 30   ctg cgg atg gtc ctc tat acc gag ttg ctg ctg ctg gac agt ctc tcg 144 Leu Arg Met Val Leu Tyr Thr Glu Leu Leu Leu Leu Asp Ser Leu Ser          35 40 45   atc ctg gcc gga ttc cac gtc gcg gcg ggc acg cgc gac ggc aac tgg 192 Ile Leu Ala Gly Phe His Val Ala Ala Gly Thr Arg Asp Gly Asn Trp      50 55 60   ctg tcg ctg gcg ggc atc aac gtc ggc gtc ttc ctg ctg ccg atc gct 240 Leu Ser Leu Ala Gly Ile Asn Val Gly Val Phe Leu Leu Pro Ile Ala  65 70 75 80   ctc ggc acc gcg ctc gca agc ggc acc tac tcg ctg aac tgc ctg cgc 288 Leu Gly Thr Ala Leu Ala Ser Gly Thr Tyr Ser Leu Asn Cys Leu Arg                  85 90 95   tac ccg gtc agc ggc gtg aag agc atc ttc tcg gca ttc ttc ttc tcg 336 Tyr Pro Val Ser Gly Val Lys Ser Ile Phe Ser Ala Phe Phe Phe Ser             100 105 110   atc ttc gtc gtc ctg ctc ggc agc tac ctg ctg acg gcc gag ctg ccg 384 Ile Phe Val Val Leu Leu Gly Ser Tyr Leu Leu Thr Ala Glu Leu Pro         115 120 125   ctg tcc cgc gtg cag ctg gcg gag ggc gcg atc ctc tcg ctg gtc ctc 432 Leu Ser Arg Val Gln Leu Ala Glu Gly Ala Ile Leu Ser Leu Val Leu     130 135 140   ctg atg gtg ggc cgc ctg atg ttc cgc cgc cac gtc cgc gcg gtt acc 480 Leu Met Val Gly Arg Leu Met Phe Arg Arg His Val Arg Ala Val Thr 145 150 155 160   ggc ggc agg ctg ctc gac gaa ctg gtc atc atc gac ggc gtc tcg ctc 528 Gly Gly Arg Leu Leu Asp Glu Leu Val Ile Ile Asp Gly Val Ser Leu                 165 170 175   gac gtc gcg ggc aat gcg gtc gcg ctc gac gcg cgg atc atc aat ctc 576 Asp Val Ala Gly Asn Ala Val Ala Leu Asp Ala Arg Ile Ile Asn Leu             180 185 190   tcg ccg aac ccg cgc gat ccg caa atg ctg cat cgc ctg ggc acc acc 624 Ser Pro Asn Pro Arg Asp Pro Gln Met Leu His Arg Leu Gly Thr Thr         195 200 205   gtg atc ggg ttc gac cgg gtg atc gtc gcc tgc acc aag gag cat cgc 672 Val Ile Gly Phe Asp Arg Val Ile Val Ala Cys Thr Lys Glu His Arg     210 215 220   gcg gtc tgg gcg ctg ctg ctc aag ggc atg aac atc aag ggc gag atc 720 Ala Val Trp Ala Leu Leu Leu Lys Gly Met Asn Ile Lys Gly Glu Ile 225 230 235 240   ctc gtc ccc cag ttc aat gcg ctg ggc gcg atc ggc gtg gac gcc ttt 768 Leu Val Pro Gln Phe Asn Ala Leu Gly Ala Ile Gly Val Asp Ala Phe                 245 250 255   gac ggg aag gat acg ctg gtc gtc tcg cag ggc ccg ctc aac atg ccc 816 Asp Gly Lys Asp Thr Leu Val Val Ser Gln Gly Pro Leu Asn Met Pro             260 265 270   aac cgc gcg aag aag cgc gcg ctc gat ctc gcg atc acc gta ccg gcc 864 Asn Arg Ala Lys Lys Arg Ala Leu Asp Leu Ala Ile Thr Val Pro Ala         275 280 285   gtg ctc gcg ctg gcg ccg ctg atg atc ctg gtg gcg atc ctg atc aag 912 Val Leu Ala Leu Ala Pro Leu Met Ile Leu Val Ala Ile Leu Ile Lys     290 295 300   ctg gag agc ccg ggc ccg gtg ttg ttc gcg cag gat cgc gtc ggc cgc 960 Leu Glu Ser Pro Gly Pro Val Leu Phe Ala Gln Asp Arg Val Gly Arg 305 310 315 320   ggc aac cgg ctg ttc aag atc atg aag ttc cgc tcg atg cgc gta acg 1008 Gly Asn Arg Leu Phe Lys Ile Met Lys Phe Arg Ser Met Arg Val Thr                 325 330 335   ctg tgc gac gcg aac ggc aac gtc tcg gcc agc cgc gac gac gat cgc 1056 Leu Cys Asp Ala Asn Gly Asn Val Ser Ala Ser Arg Asp Asp Asp Arg             340 345 350   atc acc aag gtc ggc cgc ttc atc cgc aag acc agc atc gac gaa ctg 1104 Ile Thr Lys Val Gly Arg Phe Ile Arg Lys Thr Ser Ile Asp Glu Leu         355 360 365   ccg cag ctg ctg aac gtg ctg cgc ggc gac atg agc gtc gtc ggc ccg 1152 Pro Gln Leu Leu Asn Val Leu Arg Gly Asp Met Ser Val Val Gly Pro     370 375 380   cgg ccg cat gcg ctg ggc tcg cgc gcc gcc gat cac ctg ttc tgg gaa 1200 Arg Pro His Ala Leu Gly Ser Arg Ala Ala Asp His Leu Phe Trp Glu 385 390 395 400   atc gac gag cgc tac tgg cac cgc cac acg ctc aag ccg ggc atg acc 1248 Ile Asp Glu Arg Tyr Trp His Arg His Thr Leu Lys Pro Gly Met Thr                 405 410 415   ggt ctg gcc cag gtg cgc ggt ttc cgc ggg gcg acc gat cgc cgc gtc 1296 Gly Leu Ala Gln Val Arg Gly Phe Arg Gly Ala Thr Asp Arg Arg Val             420 425 430   gat ctg acc aac cgg ctc cag gca gac atg gaa tat atc gac gga tgg 1344 Asp Leu Thr Asn Arg Leu Gln Ala Asp Met Glu Tyr Ile Asp Gly Trp         435 440 445   gat atc tgg cgc gat atc acg atc ctg ttc aag acg ctg cgg gtg atc 1392 Asp Ile Trp Arg Asp Ile Thr Ile Leu Phe Lys Thr Leu Arg Val Ile     450 455 460   gtg cat tcg aac gca ttc tga 1413 Val His Ser Asn Ala Phe * 465 470   <210> 35 <211> 470 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> glucosyl-isoprenylphosphate transferase I <400> 35 Met Asn Ala Phe Glu Ala Gln Arg Ala Phe Glu Glu Gln Leu Arg Ala  1 5 10 15 His Ser Arg Val Thr Pro Ser Ala Ala Pro Val Trp Arg Arg Ser Thr             20 25 30 Leu Arg Met Val Leu Tyr Thr Glu Leu Leu Leu Leu Asp Ser Leu Ser         35 40 45 Ile Leu Ala Gly Phe His Val Ala Ala Gly Thr Arg Asp Gly Asn Trp     50 55 60 Leu Ser Leu Ala Gly Ile Asn Val Gly Val Phe Leu Leu Pro Ile Ala 65 70 75 80 Leu Gly Thr Ala Leu Ala Ser Gly Thr Tyr Ser Leu Asn Cys Leu Arg                 85 90 95 Tyr Pro Val Ser Gly Val Lys Ser Ile Phe Ser Ala Phe Phe Phe Ser             100 105 110 Ile Phe Val Val Leu Leu Gly Ser Tyr Leu Leu Thr Ala Glu Leu Pro         115 120 125 Leu Ser Arg Val Gln Leu Ala Glu Gly Ala Ile Leu Ser Leu Val Leu     130 135 140 Leu Met Val Gly Arg Leu Met Phe Arg Arg His Val Arg Ala Val Thr 145 150 155 160 Gly Gly Arg Leu Leu Asp Glu Leu Val Ile Ile Asp Gly Val Ser Leu                 165 170 175 Asp Val Ala Gly Asn Ala Val Ala Leu Asp Ala Arg Ile Ile Asn Leu             180 185 190 Ser Pro Asn Pro Arg Asp Pro Gln Met Leu His Arg Leu Gly Thr Thr         195 200 205 Val Ile Gly Phe Asp Arg Val Ile Val Ala Cys Thr Lys Glu His Arg     210 215 220 Ala Val Trp Ala Leu Leu Leu Lys Gly Met Asn Ile Lys Gly Glu Ile 225 230 235 240 Leu Val Pro Gln Phe Asn Ala Leu Gly Ala Ile Gly Val Asp Ala Phe                 245 250 255 Asp Gly Lys Asp Thr Leu Val Val Ser Gln Gly Pro Leu Asn Met Pro             260 265 270 Asn Arg Ala Lys Lys Arg Ala Leu Asp Leu Ala Ile Thr Val Pro Ala         275 280 285 Val Leu Ala Leu Ala Pro Leu Met Ile Leu Val Ala Ile Leu Ile Lys     290 295 300 Leu Glu Ser Pro Gly Pro Val Leu Phe Ala Gln Asp Arg Val Gly Arg 305 310 315 320 Gly Asn Arg Leu Phe Lys Ile Met Lys Phe Arg Ser Met Arg Val Thr                 325 330 335 Leu Cys Asp Ala Asn Gly Asn Val Ser Ala Ser Arg Asp Asp Asp Arg             340 345 350 Ile Thr Lys Val Gly Arg Phe Ile Arg Lys Thr Ser Ile Asp Glu Leu         355 360 365 Pro Gln Leu Leu Asn Val Leu Arg Gly Asp Met Ser Val Val Gly Pro     370 375 380 Arg Pro His Ala Leu Gly Ser Arg Ala Ala Asp His Leu Phe Trp Glu 385 390 395 400 Ile Asp Glu Arg Tyr Trp His Arg His Thr Leu Lys Pro Gly Met Thr                 405 410 415 Gly Leu Ala Gln Val Arg Gly Phe Arg Gly Ala Thr Asp Arg Arg Val             420 425 430 Asp Leu Thr Asn Arg Leu Gln Ala Asp Met Glu Tyr Ile Asp Gly Trp         435 440 445 Asp Ile Trp Arg Asp Ile Thr Ile Leu Phe Lys Thr Leu Arg Val Ile     450 455 460 Val His Ser Asn Ala Phe 465 470 <210> 36 <211> 879 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (879) <223> rmlA <400> 36 atg aag ggc atc atc ctt gcg ggg ggc agc ggg acg cgc ctg tac ccc 48 Met Lys Gly Ile Ile Leu Ala Gly Gly Ser Gly Thr Arg Leu Tyr Pro  1 5 10 15   gca acg cta tcg atc tcg aag cag ctg ctt ccc gtc tat gac aag ccg 96 Ala Thr Leu Ser Ile Ser Lys Gln Leu Leu Pro Val Tyr Asp Lys Pro              20 25 30   atg atc ttc tat ccg ctg tcg gtg ctg atg ctc acc ggc atc cgg gac 144 Met Ile Phe Tyr Pro Leu Ser Val Leu Met Leu Thr Gly Ile Arg Asp          35 40 45   atc ctg att atc tcc acc ccg cgc gac ctg ccg atg ttc cag gcg ctg 192 Ile Leu Ile Ile Ser Thr Pro Arg Asp Leu Pro Met Phe Gln Ala Leu      50 55 60   ctg ggc gac ggc tcg gcc ttc ggc atc aac ctc agc tat gcc gag cag 240 Leu Gly Asp Gly Ser Ala Phe Gly Ile Asn Leu Ser Tyr Ala Glu Gln  65 70 75 80   ccc tcc ccc aac ggg ctg gcc gaa gcg ttc atc atc ggc gcg gat ttc 288 Pro Ser Pro Asn Gly Leu Ala Glu Ala Phe Ile Ile Gly Ala Asp Phe                  85 90 95   gtc ggc aac gat ccc agc gcg ctg atc ctg ggc gac aac atc tat cac 336 Val Gly Asn Asp Pro Ser Ala Leu Ile Leu Gly Asp Asn Ile Tyr His             100 105 110   ggc gaa aag atg ggc gag cgc tgc cag gca gcc gca gcg cag gca gcg 384 Gly Glu Lys Met Gly Glu Arg Cys Gln Ala Ala Ala Ala Gln Ala Ala         115 120 125   cag ggc ggt gca aac gtc ttc gcc tat cat gtc gac gac ccc gag cgc 432 Gln Gly Gly Ala Asn Val Phe Ala Tyr His Val Asp Asp Pro Glu Arg     130 135 140   tac ggc gtg gtc gcg ttc gac ccg gag acg ggc gtc gcc acc agc gtc 480 Tyr Gly Val Val Ala Phe Asp Pro Glu Thr Gly Val Ala Thr Ser Val 145 150 155 160   gag gaa aag ccg gcc gag ccc aag tcc aac tgg gcg atc acc ggc ctg 528 Glu Glu Lys Pro Ala Glu Pro Lys Ser Asn Trp Ala Ile Thr Gly Leu                 165 170 175   tat ttc tac gac aag gac gtg gtc gac atc gcc aag tcg atc cag ccc 576 Tyr Phe Tyr Asp Lys Asp Val Val Asp Ile Ala Lys Ser Ile Gln Pro             180 185 190   tcg gcg cgc ggc gaa ctc gag atc acc gac gtc aac cgc gtt tac atg 624 Ser Ala Arg Gly Glu Leu Glu Ile Thr Asp Val Asn Arg Val Tyr Met         195 200 205   gag cgc ggc gac ctg cac atc acg cgc ctc ggc cgc ggc tat gcc tgg 672 Glu Arg Gly Asp Leu His Ile Thr Arg Leu Gly Arg Gly Tyr Ala Trp     210 215 220   ctc gac acc ggc acg cat gac agc ctg cac gaa gcc ggc tcg ttc gtt 720 Leu Asp Thr Gly Thr His Asp Ser Leu His Glu Ala Gly Ser Phe Val 225 230 235 240   cgc acg ctc gag cat cgg acg ggc gtg aag atc gcc tgc ccg gag gaa 768 Arg Thr Leu Glu His Arg Thr Gly Val Lys Ile Ala Cys Pro Glu Glu                 245 250 255   atc gcc ttc gaa agc ggc tgg ctc ggc gcc gaa gac ctg ctc aag cgc 816 Ile Ala Phe Glu Ser Gly Trp Leu Gly Ala Glu Asp Leu Leu Lys Arg             260 265 270   gcc gcc ggc ctc ggc aag acc ggc tat gcc gcc tat ctc cgc aag gtt 864 Ala Ala Gly Leu Gly Lys Thr Gly Tyr Ala Ala Tyr Leu Arg Lys Val         275 280 285   gcg acc gca gca tga 879 Ala Thr Ala Ala *     290   <210> 37 <211> 292 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> glucose-1-phosphate thymidylyltransferase <400> 37 Met Lys Gly Ile Ile Leu Ala Gly Gly Ser Gly Thr Arg Leu Tyr Pro  1 5 10 15 Ala Thr Leu Ser Ile Ser Lys Gln Leu Leu Pro Val Tyr Asp Lys Pro             20 25 30 Met Ile Phe Tyr Pro Leu Ser Val Leu Met Leu Thr Gly Ile Arg Asp         35 40 45 Ile Leu Ile Ile Ser Thr Pro Arg Asp Leu Pro Met Phe Gln Ala Leu     50 55 60 Leu Gly Asp Gly Ser Ala Phe Gly Ile Asn Leu Ser Tyr Ala Glu Gln 65 70 75 80 Pro Ser Pro Asn Gly Leu Ala Glu Ala Phe Ile Ile Gly Ala Asp Phe                 85 90 95 Val Gly Asn Asp Pro Ser Ala Leu Ile Leu Gly Asp Asn Ile Tyr His             100 105 110 Gly Glu Lys Met Gly Glu Arg Cys Gln Ala Ala Ala Ala Gln Ala Ala         115 120 125 Gln Gly Gly Ala Asn Val Phe Ala Tyr His Val Asp Asp Pro Glu Arg     130 135 140 Tyr Gly Val Val Ala Phe Asp Pro Glu Thr Gly Val Ala Thr Ser Val 145 150 155 160 Glu Glu Lys Pro Ala Glu Pro Lys Ser Asn Trp Ala Ile Thr Gly Leu                 165 170 175 Tyr Phe Tyr Asp Lys Asp Val Val Asp Ile Ala Lys Ser Ile Gln Pro             180 185 190 Ser Ala Arg Gly Glu Leu Glu Ile Thr Asp Val Asn Arg Val Tyr Met         195 200 205 Glu Arg Gly Asp Leu His Ile Thr Arg Leu Gly Arg Gly Tyr Ala Trp     210 215 220 Leu Asp Thr Gly Thr His Asp Ser Leu His Glu Ala Gly Ser Phe Val 225 230 235 240 Arg Thr Leu Glu His Arg Thr Gly Val Lys Ile Ala Cys Pro Glu Glu                 245 250 255 Ile Ala Phe Glu Ser Gly Trp Leu Gly Ala Glu Asp Leu Leu Lys Arg             260 265 270 Ala Ala Gly Leu Gly Lys Thr Gly Tyr Ala Ala Tyr Leu Arg Lys Val         275 280 285 Ala Thr Ala Ala     290 <210> 38 <211> 567 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS <222> (1) ... (567) <223> rmlC <400> 38 atg acc cag gtc cat cat cac gaa ctg tcc ggc gtc atc gag ttc acg 48 Met Thr Gln Val His His His Glu Leu Ser Gly Val Ile Glu Phe Thr  1 5 10 15   ccg ccc aaa tat ggc gac cac cgc ggc ttc ttc tcc gaa gtg ttc aag 96 Pro Pro Lys Tyr Gly Asp His Arg Gly Phe Phe Ser Glu Val Phe Lys              20 25 30   cag tcg gtg ctc gat gcc gaa ggc gtc gag gca cgc tgg gtg cag gac 144 Gln Ser Val Leu Asp Ala Glu Gly Val Glu Ala Arg Trp Val Gln Asp          35 40 45   aat cag agc ttc tcg gcg gcc ccg ggc acg atc cgc ggc ctg cat ctc 192 Asn Gln Ser Phe Ser Ala Ala Pro Gly Thr Ile Arg Gly Leu His Leu      50 55 60   cag gcg ccg ccc ttc gcc cag gcc aag ctg gtc cgc gtg ttg cgc ggc 240 Gln Ala Pro Pro Phe Ala Gln Ala Lys Leu Val Arg Val Leu Arg Gly  65 70 75 80   gcg atc ttc gac gtc gcg gtc gac atc cgt cgc ggc tcg ccc acc tat 288 Ala Ile Phe Asp Val Ala Val Asp Ile Arg Arg Gly Ser Pro Thr Tyr                  85 90 95   ggc aaa tgg gtc ggc gtc gag ctc tcg gcc gag aag tgg aac cag ctg 336 Gly Lys Trp Val Gly Val Glu Leu Ser Ala Glu Lys Trp Asn Gln Leu             100 105 110   ctg gtc ccc gcc ggc tat gcg cac ggc ttc atg acg ctc gtt ccg gat 384 Leu Val Pro Ala Gly Tyr Ala His Gly Phe Met Thr Leu Val Pro Asp         115 120 125   tgc gag atc ctc tac aag gtc agc gcc aaa tat tcg aag gat tcg gag 432 Cys Glu Ile Leu Tyr Lys Val Ser Ala Lys Tyr Ser Lys Asp Ser Glu     130 135 140   atg gcg atc cgt tgg gac gat ccc gat ctc gcc atc gcc tgg ccg gac 480 Met Ala Ile Arg Trp Asp Asp Pro Asp Leu Ala Ile Ala Trp Pro Asp 145 150 155 160   atc ggc gtc gag ccg gtc ctc tcc gaa aag gac gcg gtc gcc acg ccc 528 Ile Gly Val Glu Pro Val Leu Ser Glu Lys Asp Ala Val Ala Thr Pro                 165 170 175   ttc gcc gaa ttc aac acc ccc ttc ttc tat cag ggc tga 567 Phe Ala Glu Phe Asn Thr Pro Phe Phe Tyr Gln Gly *             180 185   <210> 39 <211> 188 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> dTDP-6-deoxy-D-glucose-3-5-epimerase <400> 39 Met Thr Gln Val His His His Glu Leu Ser Gly Val Ile Glu Phe Thr  1 5 10 15 Pro Pro Lys Tyr Gly Asp His Arg Gly Phe Phe Ser Glu Val Phe Lys             20 25 30 Gln Ser Val Leu Asp Ala Glu Gly Val Glu Ala Arg Trp Val Gln Asp         35 40 45 Asn Gln Ser Phe Ser Ala Ala Pro Gly Thr Ile Arg Gly Leu His Leu     50 55 60 Gln Ala Pro Pro Phe Ala Gln Ala Lys Leu Val Arg Val Leu Arg Gly 65 70 75 80 Ala Ile Phe Asp Val Ala Val Asp Ile Arg Arg Gly Ser Pro Thr Tyr                 85 90 95 Gly Lys Trp Val Gly Val Glu Leu Ser Ala Glu Lys Trp Asn Gln Leu             100 105 110 Leu Val Pro Ala Gly Tyr Ala His Gly Phe Met Thr Leu Val Pro Asp         115 120 125 Cys Glu Ile Leu Tyr Lys Val Ser Ala Lys Tyr Ser Lys Asp Ser Glu     130 135 140 Met Ala Ile Arg Trp Asp Asp Pro Asp Leu Ala Ile Ala Trp Pro Asp 145 150 155 160 Ile Gly Val Glu Pro Val Leu Ser Glu Lys Asp Ala Val Ala Thr Pro                 165 170 175 Phe Ala Glu Phe Asn Thr Pro Phe Phe Tyr Gln Gly             180 185 <210> 40 <211> 1062 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS <222> (1) ... (1062) <223> rmlB <400> 40 atg cag cag acc ttc ctc gtc acc ggc ggc gcc ggc ttc atc ggc tcg 48 Met Gln Gln Thr Phe Leu Val Thr Gly Gly Ala Gly Phe Ile Gly Ser  1 5 10 15   gcg gtg gtg cgc cac ctc gtc cgc cag ggc gcg cgc gtc atc aat ctc 96 Ala Val Val Arg His Leu Val Arg Gln Gly Ala Arg Val Ile Asn Leu              20 25 30   gac aag ctc acc tat gcc ggc aac ccg gcc tcg ctg act gcg atc gag 144 Asp Lys Leu Thr Tyr Ala Gly Asn Pro Ala Ser Leu Thr Ala Ile Glu          35 40 45   aac gcg ccc aac tat cgc ttc gtc cat gcc gac atc gcc gac acc gcg 192 Asn Ala Pro Asn Tyr Arg Phe Val His Ala Asp Ile Ala Asp Thr Ala      50 55 60   acg atc cta ccg ctg ctg cgc gag gag cag gtc gat gtg gtg atg cac 240 Thr Ile Leu Pro Leu Leu Arg Glu Glu Gln Val Asp Val Val Met His  65 70 75 80   ctc gcc gcc gag agc cat gtc gat cgc tcg atc gac ggc cct ggc gag 288 Leu Ala Ala Glu Ser His Val Asp Arg Ser Ile Asp Gly Pro Gly Glu                  85 90 95   ttc atc gag acc aat gtc gtc ggc acc ttc aag ctg ctc cag tcg gcg 336 Phe Ile Glu Thr Asn Val Val Gly Thr Phe Lys Leu Leu Gln Ser Ala             100 105 110   ctg caa tat tgg cgc gag ctg gag ggc gag aaa cgc gac gcg ttc cgc 384 Leu Gln Tyr Trp Arg Glu Leu Glu Gly Glu Lys Arg Asp Ala Phe Arg         115 120 125   ttc cac cac atc tcc acc gac gaa gtg ttc ggc gac ctg ccg ttc gac 432 Phe His His Ile Ser Thr Asp Glu Val Phe Gly Asp Leu Pro Phe Asp     130 135 140   agc ggc atc ttc acc gaa gag acg ccc tat gat ccc tcc tcg ccc tat 480 Ser Gly Ile Phe Thr Glu Glu Thr Pro Tyr Asp Pro Ser Ser Pro Tyr 145 150 155 160   tcg gcg tcg aag gcg gcg agc gac cat ctg gtg cgc gcc tgg ggc cac 528 Ser Ala Ser Lys Ala Ala Ser Asp His Leu Val Arg Ala Trp Gly His                 165 170 175   acc tat ggc ctg ccg gtg gtg ctg tcg aac tgc tcg aac aat tac ggg 576 Thr Tyr Gly Leu Pro Val Val Leu Ser Asn Cys Ser Asn Asn Tyr Gly             180 185 190   ccg ttc cac ttc ccc gag aag ctg atc ccg ttg acc atc ctc aac gcg 624 Pro Phe His Phe Pro Glu Lys Leu Ile Pro Leu Thr Ile Leu Asn Ala         195 200 205   ctc gag ggc aag ccg ctg ccg gtc tac ggc aag ggc gag aat atc cgc 672 Leu Glu Gly Lys Pro Leu Pro Val Tyr Gly Lys Gly Glu Asn Ile Arg     210 215 220   gac tgg ctg tat gtc gac gat cac gcc aag gcg ctg gcg acc atc gcc 720 Asp Trp Leu Tyr Val Asp Asp His Ala Lys Ala Leu Ala Thr Ile Ala 225 230 235 240   acc acc ggc aag gtc ggc cag agc tac aat gtc ggc ggc cgc aac gag 768 Thr Thr Gly Lys Val Gly Gln Ser Tyr Asn Val Gly Gly Arg Asn Glu                 245 250 255   cgg acc aac ctg cag gtg gtc gag acg atc tgc gac ctg ctc gac cag 816 Arg Thr Asn Leu Gln Val Val Glu Thr Ile Cys Asp Leu Leu Asp Gln             260 265 270   cgc att ccg ctg gcc gac ggt cgc aag cgc cgc gaa ctg atc acc ttc 864 Arg Ile Pro Leu Ala Asp Gly Arg Lys Arg Arg Glu Leu Ile Thr Phe         275 280 285   gtc acc gat cgc ccc ggc cat gac cgc cgc tac gcg atc gac gcg acc 912 Val Thr Asp Arg Pro Gly His Asp Arg Arg Tyr Ala Ile Asp Ala Thr     290 295 300   aag ctc gag acc gag ctg ggc tgg aag gct gag gag aat ttc gac acc 960 Lys Leu Glu Thr Glu Leu Gly Trp Lys Ala Glu Glu Asn Phe Asp Thr 305 310 315 320   ggc atc gcc gcg acg atc gac tgg tat ctg gcg aac gag tgg tgg tgg 1008 Gly Ile Ala Ala Thr Ile Asp Trp Tyr Leu Ala Asn Glu Trp Trp Trp                 325 330 335   ggc ccg atc cgc tcc ggc aaa tat gcc ggc gag cgg ctg ggg cag acc 1056 Gly Pro Ile Arg Ser Gly Lys Tyr Ala Gly Glu Arg Leu Gly Gln Thr             340 345 350   gcc tga 1062 Ala *   <210> 41 <211> 353 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> dTDP-D-glucose-4,6-dehydratase <400> 41 Met Gln Gln Thr Phe Leu Val Thr Gly Gly Ala Gly Phe Ile Gly Ser  1 5 10 15 Ala Val Val Arg His Leu Val Arg Gln Gly Ala Arg Val Ile Asn Leu             20 25 30 Asp Lys Leu Thr Tyr Ala Gly Asn Pro Ala Ser Leu Thr Ala Ile Glu         35 40 45 Asn Ala Pro Asn Tyr Arg Phe Val His Ala Asp Ile Ala Asp Thr Ala     50 55 60 Thr Ile Leu Pro Leu Leu Arg Glu Glu Gln Val Asp Val Val Met His 65 70 75 80 Leu Ala Ala Glu Ser His Val Asp Arg Ser Ile Asp Gly Pro Gly Glu                 85 90 95 Phe Ile Glu Thr Asn Val Val Gly Thr Phe Lys Leu Leu Gln Ser Ala             100 105 110 Leu Gln Tyr Trp Arg Glu Leu Glu Gly Glu Lys Arg Asp Ala Phe Arg         115 120 125 Phe His His Ile Ser Thr Asp Glu Val Phe Gly Asp Leu Pro Phe Asp     130 135 140 Ser Gly Ile Phe Thr Glu Glu Thr Pro Tyr Asp Pro Ser Ser Pro Tyr 145 150 155 160 Ser Ala Ser Lys Ala Ala Ser Asp His Leu Val Arg Ala Trp Gly His                 165 170 175 Thr Tyr Gly Leu Pro Val Val Leu Ser Asn Cys Ser Asn Asn Tyr Gly             180 185 190 Pro Phe His Phe Pro Glu Lys Leu Ile Pro Leu Thr Ile Leu Asn Ala         195 200 205 Leu Glu Gly Lys Pro Leu Pro Val Tyr Gly Lys Gly Glu Asn Ile Arg     210 215 220 Asp Trp Leu Tyr Val Asp Asp His Ala Lys Ala Leu Ala Thr Ile Ala 225 230 235 240 Thr Thr Gly Lys Val Gly Gln Ser Tyr Asn Val Gly Gly Arg Asn Glu                 245 250 255 Arg Thr Asn Leu Gln Val Val Glu Thr Ile Cys Asp Leu Leu Asp Gln             260 265 270 Arg Ile Pro Leu Ala Asp Gly Arg Lys Arg Arg Glu Leu Ile Thr Phe         275 280 285 Val Thr Asp Arg Pro Gly His Asp Arg Arg Tyr Ala Ile Asp Ala Thr     290 295 300 Lys Leu Glu Thr Glu Leu Gly Trp Lys Ala Glu Glu Asn Phe Asp Thr 305 310 315 320 Gly Ile Ala Ala Thr Ile Asp Trp Tyr Leu Ala Asn Glu Trp Trp Trp                 325 330 335 Gly Pro Ile Arg Ser Gly Lys Tyr Ala Gly Glu Arg Leu Gly Gln Thr             340 345 350 Ala <210> 42 <211> 867 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS (222) (1) ... (867) <223> rmlD <400> 42 atg cgt atc ctc gtc acc ggg cat gac ggc cag gtc gcc cag tcg ctg 48 Met Arg Ile Leu Val Thr Gly His Asp Gly Gln Val Ala Gln Ser Leu  1 5 10 15   gcc gag cag gcg gtg ggc cac gag ctg gtc ttc acc acc tac ccc gaa 96 Ala Glu Gln Ala Val Gly His Glu Leu Val Phe Thr Thr Tyr Pro Glu              20 25 30   ttc gat ctc tcc aag ccg gag acg atc gag gcc ggt gtg gcg cgg gtg 144 Phe Asp Leu Ser Lys Pro Glu Thr Ile Glu Ala Gly Val Ala Arg Val          35 40 45   cac ccg gac ctg atc gtc tcc gcc gcc gcc tac acg gcg gtc gac aag 192 His Pro Asp Leu Ile Val Ser Ala Ala Ala Tyr Thr Ala Val Asp Lys      50 55 60   gcg gaa agc gaa ccc gag ctg gcg atg gcg atc aac ggc gac ggt ccc 240 Ala Glu Ser Glu Pro Glu Leu Ala Met Ala Ile Asn Gly Asp Gly Pro  65 70 75 80   ggc gtg ctg gcg cgc gcg ggc gcg aag atc ggc gcg ccg atc atc cac 288 Gly Val Leu Ala Arg Ala Gly Ala Lys Ile Gly Ala Pro Ile Ile His                  85 90 95   ctg tcg acc gat tat gtg ttc gac ggc agt ctc gac cgc cct tgg cgc 336 Leu Ser Thr Asp Tyr Val Phe Asp Gly Ser Leu Asp Arg Pro Trp Arg             100 105 110   gag gac gat ccc acc ggc ccg ctc ggc gtc tat ggc gcg acc aag ctg 384 Glu Asp Asp Pro Thr Gly Pro Leu Gly Val Tyr Gly Ala Thr Lys Leu         115 120 125   gcc ggc gag cag gcg gtg cag gcc tcg ggt gcc acc aac gcc gtg atc 432 Ala Gly Glu Gln Ala Val Gln Ala Ser Gly Ala Thr Asn Ala Val Ile     130 135 140   cgg ctg gcc tgg gtc tac agc ccg ttc ggc aac aat ttc gtc aag acg 480 Arg Leu Ala Trp Val Tyr Ser Pro Phe Gly Asn Asn Phe Val Lys Thr 145 150 155 160   atg ctc cgc ctc gcc gag acg cgc gac gcg ctg aac gtc gtg gag gac 528 Met Leu Arg Leu Ala Glu Thr Arg Asp Ala Leu Asn Val Val Glu Asp                 165 170 175   cag tgg ggc tgc ccc agt tcg gcg ctg gac atc gcg acc gcg atc ctg 576 Gln Trp Gly Cys Pro Ser Ser Ala Leu Asp Ile Ala Thr Ala Ile Leu             180 185 190   acg gtg gtc ggg cac tgg cag cag gac ggc gcg acg agc ggc ctc tac 624 Thr Val Val Gly His Trp Gln Gln Asp Gly Ala Thr Ser Gly Leu Tyr         195 200 205   cat ttc gcc ggc acc ggc gag acc aac tgg gcc gac ttc gca tcg acg 672 His Phe Ala Gly Thr Gly Glu Thr Asn Trp Ala Asp Phe Ala Ser Thr     210 215 220   atc ttc gcc gag agc gcc aag cgc ggt ggc ccc tcg gcc acc gtc acc 720 Ile Phe Ala Glu Ser Ala Lys Arg Gly Gly Pro Ser Ala Thr Val Thr 225 230 235 240   ggc att ccc agc tcg ggc tat ccg act ccg gcc acg cgc ccg gcc aat 768 Gly Ile Pro Ser Ser Gly Tyr Pro Thr Pro Ala Thr Arg Pro Ala Asn                 245 250 255   tcg cgg ctg gac tgc acc cgc ttc gcg gag acc ttc ggc tac cgg gcg 816 Ser Arg Leu Asp Cys Thr Arg Phe Ala Glu Thr Phe Gly Tyr Arg Ala             260 265 270   cct gcc tgg cag gat tcg ctg aac gtc gta ctg gat cgc ctg ctc ggc 864 Pro Ala Trp Gln Asp Ser Leu Asn Val Val Leu Asp Arg Leu Leu Gly         275 280 285   tga 867  *   <210> 43 <211> 288 <212> PRT <213> Sphingomonas sp. ATCC53159 <220> <221> PEPTIDE <222> (0) (0) <223> dTDP-6-deoxy-L-mannose-dehydrogenase <400> 43 Met Arg Ile Leu Val Thr Gly His Asp Gly Gln Val Ala Gln Ser Leu  1 5 10 15 Ala Glu Gln Ala Val Gly His Glu Leu Val Phe Thr Thr Tyr Pro Glu             20 25 30 Phe Asp Leu Ser Lys Pro Glu Thr Ile Glu Ala Gly Val Ala Arg Val         35 40 45 His Pro Asp Leu Ile Val Ser Ala Ala Ala Tyr Thr Ala Val Asp Lys     50 55 60 Ala Glu Ser Glu Pro Glu Leu Ala Met Ala Ile Asn Gly Asp Gly Pro 65 70 75 80 Gly Val Leu Ala Arg Ala Gly Ala Lys Ile Gly Ala Pro Ile Ile His                 85 90 95 Leu Ser Thr Asp Tyr Val Phe Asp Gly Ser Leu Asp Arg Pro Trp Arg             100 105 110 Glu Asp Asp Pro Thr Gly Pro Leu Gly Val Tyr Gly Ala Thr Lys Leu         115 120 125 Ala Gly Glu Gln Ala Val Gln Ala Ser Gly Ala Thr Asn Ala Val Ile     130 135 140 Arg Leu Ala Trp Val Tyr Ser Pro Phe Gly Asn Asn Phe Val Lys Thr 145 150 155 160 Met Leu Arg Leu Ala Glu Thr Arg Asp Ala Leu Asn Val Val Glu Asp                 165 170 175 Gln Trp Gly Cys Pro Ser Ser Ala Leu Asp Ile Ala Thr Ala Ile Leu             180 185 190 Thr Val Val Gly His Trp Gln Gln Asp Gly Ala Thr Ser Gly Leu Tyr         195 200 205 His Phe Ala Gly Thr Gly Glu Thr Asn Trp Ala Asp Phe Ala Ser Thr     210 215 220 Ile Phe Ala Glu Ser Ala Lys Arg Gly Gly Pro Ser Ala Thr Val Thr 225 230 235 240 Gly Ile Pro Ser Ser Gly Tyr Pro Thr Pro Ala Thr Arg Pro Ala Asn                 245 250 255 Ser Arg Leu Asp Cys Thr Arg Phe Ala Glu Thr Phe Gly Tyr Arg Ala             260 265 270 Pro Ala Trp Gln Asp Ser Leu Asn Val Val Leu Asp Arg Leu Leu Gly         275 280 285 <210> 44 <211> 132 <212> DNA <213> Sphingomonas sp. ATCC53159 <220> <221> CDS <222> (1) ... (132) Orf7 (partial); unknown function <400> 2 atc cgg ctg tgc ctg ggg tgc tgg cgg tcg ccc aag gaa atc gcc ggc 48 Ile Arg Leu Cys Leu Gly Cys Trp Arg Ser Pro Lys Glu Ile Ala Gly  1 5 10 15   tgg agc gag ctg agt cct aag gga aag cgc gcg gtg cta gag gca ttg 96 Trp Ser Glu Leu Ser Pro Lys Gly Lys Arg Ala Val Leu Glu Ala Leu              20 25 30   ccg gcg cgc gaa cgg gag cat ggc ggg ggg cgc tga 132 Pro Ala Arg Glu Arg Glu His Gly Gly Gly Arg *          35 40 <210> 45 <211> 43 <212> PRT <213> Sphingomonas sp. ATCC53159 <400> 3 Ile Arg Leu Cys Leu Gly Cys Trp Arg Ser Pro Lys Glu Ile Ala Gly  1 5 10 15 Trp Ser Glu Leu Ser Pro Lys Gly Lys Arg Ala Val Leu Glu Ala Leu             20 25 30 Pro Ala Arg Glu Arg Glu His Gly Gly Gly Arg         35 40

Claims (34)

Diutan gum exhibiting an intrinsic viscosity greater than 150 dL / g when plotting the reduced viscosity against solution concentration and extrapolating the linear regression of the data to zero concentration. After mixing the sample gum with the synthetic seawater and mixing, the solution is cooled to about 26 ° C. and placed on a fan sample flatform whose speed is adjusted to 3 rpm, and read from the dial to obtain a scale value. dial reading) Diutan gum with a viscosity of 3 rpm above seawater. Using a Brookfield viscometer with LV-2C spindle to measure the viscosity, the scale value is obtained, the speed of the spindle is set to 0.3 rpm, and if the spindle is rotated for more than 6 minutes before recording the viscosity, Diutan gum showing 0.3 rpm viscosity of seawater above 35,000 cP. 0.25% of biogum in standard tap water (STW) using a Brookfield LV viscometer with 2.5+ torque spring at 3 rpm using the LV 1 spindle for 3 minutes Diutan gum, which shows a low shear rate viscosity of greater than 3,500 cP in the presence of a polyethylene glycol dispersant when measuring the viscosity by measuring the viscosity of a solution. Strain ATCC No. Introducing a plasmid comprising the nucleic acid sequence according to SEQ ID NO: 1 into the Diutan producing Sphingomonas host organism of 53159; Culturing the host organism under fermentation conditions, wherein the host organism a) inherent viscosity greater than 150 dL / g when plotting the reduced viscosity against solution concentration and extrapolating linear regression of the data to zero concentration; b) The sample gum is combined with synthetic seawater and mixed, and the solution is cooled to about 26 ° C. and placed on a fan sample flatform whose speed is adjusted to 3 rpm and read from the dial to obtain a scale value. Seawater 3 rpm viscosity above 35 reading; c) A Brookfield viscometer with LV-2C spindle is used to measure the viscosity, and the spindle speed is set to 0.3 rpm and the spindle is rotated for at least 6 minutes before recording the viscosity. If present, 0.3 rpm viscosity of seawater above 35,000 cP; And d) Biogum in Standard Tap Water (STW) using a Brookfield LV viscometer with a 2.5+ torque spring at 3 rpm using the LV 1 spindle rotated for 3 minutes. When the viscosity is measured by measuring the viscosity of a 0.25% solution, a low shear rate viscosity of greater than 3,500 cP in the presence of a polyethylene glycol dispersant A method for producing diutan gum, which produces diutan gum that exhibits at least one of the following properties. delete delete delete delete delete delete delete delete delete delete delete delete delete An isolated nucleic acid molecule comprising the nucleic acid sequence according to SEQ ID NO: 1. delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete

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