DK3635136T3 - Enkeltcelle-helgenombiblioteker til methyleringssekvensering - Google Patents

Enkeltcelle-helgenombiblioteker til methyleringssekvensering Download PDF

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DK3635136T3
DK3635136T3 DK18734381.9T DK18734381T DK3635136T3 DK 3635136 T3 DK3635136 T3 DK 3635136T3 DK 18734381 T DK18734381 T DK 18734381T DK 3635136 T3 DK3635136 T3 DK 3635136T3
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weekend
libraries
single cell
methylation sequence
methylation
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DK18734381.9T
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Danish (da)
English (en)
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Andrew C Adey
Ryan Mulqueen
Frank J Steemers
Dmitry K Pokholok
Steven Norberg
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Univ Oregon Health & Science
Illumina Inc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C40—COMBINATORIAL TECHNOLOGY
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    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/159—Reduction of complexity, e.g. amplification of subsets, removing duplicated genomic regions
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • General Health & Medical Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK18734381.9T 2017-06-07 2018-06-05 Enkeltcelle-helgenombiblioteker til methyleringssekvensering DK3635136T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762516324P 2017-06-07 2017-06-07
PCT/US2018/036078 WO2018226708A1 (en) 2017-06-07 2018-06-05 Single cell whole genome libraries for methylation sequencing

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DK3635136T3 true DK3635136T3 (da) 2022-01-10

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DK21203166.0T DK3981884T3 (da) 2017-06-07 2018-06-05 Enkeltcelle-helgenombiblioteker til methyleringssekventering

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EP (3) EP3981884B1 (cg-RX-API-DMAC7.html)
JP (2) JP7407597B2 (cg-RX-API-DMAC7.html)
KR (2) KR20240013852A (cg-RX-API-DMAC7.html)
CN (2) CN118497317A (cg-RX-API-DMAC7.html)
AU (1) AU2018280131B2 (cg-RX-API-DMAC7.html)
CA (1) CA3066424A1 (cg-RX-API-DMAC7.html)
DK (2) DK3635136T3 (cg-RX-API-DMAC7.html)
ES (3) ES3014783T3 (cg-RX-API-DMAC7.html)
FI (1) FI3981884T3 (cg-RX-API-DMAC7.html)
IL (2) IL271215B2 (cg-RX-API-DMAC7.html)
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