DK3329012T3 - Rumlig kortlægning af nukleinsyresekvensinformation - Google Patents

Rumlig kortlægning af nukleinsyresekvensinformation Download PDF

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DK3329012T3
DK3329012T3 DK16750552.8T DK16750552T DK3329012T3 DK 3329012 T3 DK3329012 T3 DK 3329012T3 DK 16750552 T DK16750552 T DK 16750552T DK 3329012 T3 DK3329012 T3 DK 3329012T3
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Denmark
Prior art keywords
nucleic acid
acid sequence
sequence information
spatial mapping
mapping
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DK16750552.8T
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English (en)
Inventor
Alex So
Li Liu
Min-Jui Richard Shen
Neeraj Salathia
Kathryn M Stephens
Anne Jager
Timothy Wilson
Justin Fullerton
Sean M Ramirez
Shannon Kaplan
Rigo Pantoja
Bala Murali Venkatesan
Steven Modiano
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Illumina Inc
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2539/00Reactions characterised by analysis of gene expression or genome comparison
    • C12Q2539/10The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
    • C12Q2539/115Comparative genomic hybridisation [CGH]
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    • C12Q2543/00Reactions characterised by the reaction site, e.g. cell or chromosome
    • C12Q2543/10Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis
    • C12Q2543/101Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis in situ amplification
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    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/514Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
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    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/519Detection characterised by immobilisation to a surface characterised by the capture moiety being a single stranded oligonucleotide
    • CCHEMISTRY; METALLURGY
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    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/543Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification
    • CCHEMISTRY; METALLURGY
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
DK16750552.8T 2015-07-27 2016-07-21 Rumlig kortlægning af nukleinsyresekvensinformation DK3329012T3 (da)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201562197389P 2015-07-27 2015-07-27
US201562218742P 2015-09-15 2015-09-15
US201562250329P 2015-11-03 2015-11-03
US201562261707P 2015-12-01 2015-12-01
US201562269614P 2015-12-18 2015-12-18
PCT/US2016/043385 WO2017019456A2 (en) 2015-07-27 2016-07-21 Spatial mapping of nucleic acid sequence information

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DK3329012T3 true DK3329012T3 (da) 2021-10-11

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US (2) US10913975B2 (da)
EP (2) EP3329012B1 (da)
CN (2) CN108138225B (da)
AU (3) AU2016298158B2 (da)
CA (2) CA3176469A1 (da)
DK (1) DK3329012T3 (da)
ES (1) ES2888626T3 (da)
HK (1) HK1250170A1 (da)
WO (1) WO2017019456A2 (da)

Families Citing this family (134)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2556171T3 (da) 2010-04-05 2015-12-14 Prognosys Biosciences Inc Rumligt kodede biologiske assays
EP3246416A1 (en) 2011-04-15 2017-11-22 The Johns Hopkins University Safe sequencing system
EP2912468B1 (en) 2012-10-29 2018-09-12 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
CN111662960B (zh) 2013-06-25 2024-04-12 普罗格诺西斯生物科学公司 采用微流控装置的空间编码生物分析
DK3901281T3 (da) 2015-04-10 2023-01-23 Spatial Transcriptomics Ab Rumligt adskilt, multipleks nukleinsyreanalyse af biologiske prøver
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
US11016053B2 (en) * 2016-10-05 2021-05-25 Abbott Laboratories Devices and methods for sample analysis
EP3526348A4 (en) * 2016-10-17 2020-06-24 Lociomics Corporation HIGH RESOLUTION GENOME SPATIAL ANALYSIS OF TISSUE AND CELL AGGREGATES
GB201619458D0 (en) * 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
EP3631001A4 (en) * 2017-05-23 2021-01-20 Centrillion Technology Holdings Corporation PROCESSES FOR ESTABLISHING A SPATIAL PROFILE OF BIOLOGICAL MOLECULES
SG11201911869XA (en) * 2017-08-01 2020-01-30 Illumina Inc Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
AU2018342007A1 (en) 2017-08-07 2020-02-27 Board Of Regents, The University Of Texas Systems Methods and materials for assessing and treating cancer
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020047004A2 (en) 2018-08-28 2020-03-05 10X Genomics, Inc. Methods of generating an array
WO2020047005A1 (en) 2018-08-28 2020-03-05 10X Genomics, Inc. Resolving spatial arrays
WO2020076979A1 (en) * 2018-10-10 2020-04-16 Readcoor, Inc. Surface capture of targets
WO2020123320A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Imaging system hardware
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
TW202045709A (zh) 2019-01-29 2020-12-16 美商伊路米納有限公司 流體槽
TW202043486A (zh) 2019-01-29 2020-12-01 美商伊路米納有限公司 定序套組
EP3931354A1 (en) 2019-02-28 2022-01-05 10X Genomics, Inc. Profiling of biological analytes with spatially barcoded oligonucleotide arrays
EP3938538A1 (en) 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
CN109979530B (zh) * 2019-03-26 2021-03-16 北京市商汤科技开发有限公司 一种基因变异识别方法、装置和存储介质
WO2020227543A2 (en) * 2019-05-07 2020-11-12 Fluidigm Corporation Sequence based imaging
JP2022540744A (ja) * 2019-05-15 2022-09-20 ビージーアイ シェンチェン 核酸の空間情報を検出するためのアレイ及び方法
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US20220251632A1 (en) * 2019-07-23 2022-08-11 University Of Washington Method for spatially barcoding cells in tissue slices
WO2021067514A1 (en) 2019-10-01 2021-04-08 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
US20210130881A1 (en) 2019-11-06 2021-05-06 10X Genomics, Inc. Imaging system hardware
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4058572A4 (en) * 2019-11-11 2023-11-29 The Broad Institute, Inc. HIGH RESOLUTION SPATIAL AND QUANTITATIVE DNA ASSESSMENT
US20230002812A1 (en) 2019-11-13 2023-01-05 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2021102003A1 (en) 2019-11-18 2021-05-27 10X Genomics, Inc. Systems and methods for tissue classification
EP4062373A1 (en) 2019-11-21 2022-09-28 10X Genomics, Inc. Spatial analysis of analytes
CN115023734B (zh) 2019-11-22 2023-10-20 10X基因组学有限公司 使用基准对齐对分析物进行空间分析的系统和方法
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
EP4339299A2 (en) 2020-01-10 2024-03-20 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
US20210223227A1 (en) * 2020-01-17 2021-07-22 Spatial Transcriptomics Ab Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US20230047782A1 (en) 2020-02-07 2023-02-16 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
CN115052995A (zh) * 2020-02-14 2022-09-13 索尼集团公司 分析方法、分析系统及分析表面
US20230081381A1 (en) 2020-02-20 2023-03-16 10X Genomics, Inc. METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS
AU2021224760A1 (en) 2020-02-21 2022-09-15 10X Genomics, Inc. Capturing genetic targets using a hybridization approach
US20210262018A1 (en) * 2020-02-21 2021-08-26 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4242325A3 (en) 2020-04-22 2023-10-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US20230265491A1 (en) 2020-05-04 2023-08-24 10X Genomics, Inc. Spatial transcriptomic transfer modes
US20230194469A1 (en) 2020-05-19 2023-06-22 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021237056A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Rna integrity analysis in a biological sample
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4158054A1 (en) 2020-06-02 2023-04-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US20230279474A1 (en) 2020-06-10 2023-09-07 10X Genomics, Inc. Methods for spatial analysis using blocker oligonucleotides
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
WO2021263111A1 (en) * 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
CN115768863A (zh) * 2020-07-06 2023-03-07 索尼集团公司 生物粒子分析方法和生物粒子分析系统
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11624088B2 (en) 2020-07-17 2023-04-11 13.8, Inc. Methods, compositions, and systems for mapping locations of single molecules in multi-dimensional space
EP4189110A1 (en) 2020-07-31 2023-06-07 10X Genomics, Inc. De-crosslinking compounds and methods of use for spatial analysis
AU2021344340A1 (en) 2020-09-15 2023-03-23 10X Genomics, Inc. Methods of releasing an extended capture probe from a substrate and uses of the same
EP4214333A1 (en) 2020-09-16 2023-07-26 10X Genomics, Inc. Methods of determining the location of an analyte in a biological sample using a plurality of wells
US20230351619A1 (en) 2020-09-18 2023-11-02 10X Genomics, Inc. Sample handling apparatus and image registration methods
WO2022061152A2 (en) 2020-09-18 2022-03-24 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022081643A2 (en) 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
EP4214330A1 (en) 2020-10-22 2023-07-26 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification
EP4222283A1 (en) 2020-11-06 2023-08-09 10X Genomics, Inc. Compositions and methods for binding an analyte to a capture probe
EP4247978A1 (en) 2020-11-18 2023-09-27 10X Genomics, Inc. Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220195498A1 (en) * 2020-12-23 2022-06-23 10X Genomics, Inc. Methods and compositions for analyte detection
US20240076723A1 (en) 2020-12-30 2024-03-07 10X Genomics, Inc. Cleavage of capture probes for spatial analysis
US20240068017A1 (en) 2020-12-30 2024-02-29 10X Genomics, Inc. Methods for analyte capture determination
CN116685697A (zh) * 2021-01-08 2023-09-01 安捷伦科技有限公司 使用寡核苷酸微阵列进行空间核酸检测
US20240093290A1 (en) 2021-01-29 2024-03-21 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2022212956A1 (en) * 2021-04-02 2022-10-06 Board Of Regents, The University Of Texas System Nextgen chemometrics using proximity ligation assay (pla)
WO2022216688A1 (en) 2021-04-05 2022-10-13 10X Genomics, Inc. Recombinant ligase composition and uses thereof
WO2022221425A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022226057A1 (en) 2021-04-20 2022-10-27 10X Genomics, Inc. Methods for assessing sample quality prior to spatial analysis using templated ligation
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022271820A1 (en) * 2021-06-22 2022-12-29 10X Genomics, Inc. Spatial detection of sars-cov-2 using templated ligation
WO2023287765A1 (en) 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for spatial analysis using targeted probe silencing
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
CN113604547B (zh) * 2021-08-06 2023-05-02 吉林大学 一种用于组织样本的高分辨率空间组学检测方法
WO2023018799A1 (en) 2021-08-12 2023-02-16 10X Genomics, Inc. Methods, compositions and systems for identifying antigen-binding molecules
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023044071A1 (en) 2021-09-17 2023-03-23 10X Genomics, Inc. Systems and methods for image registration or alignment
WO2023076345A1 (en) * 2021-10-26 2023-05-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna capture
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023086824A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods for identification of antigen-binding molecules
US20230167495A1 (en) 2021-11-30 2023-06-01 10X Genomics, Inc. Systems and methods for identifying regions of aneuploidy in a tissue
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023116373A1 (zh) * 2021-12-24 2023-06-29 深圳华大生命科学研究院 一种生成标记的核酸分子群的方法及其试剂盒
CN114438186A (zh) * 2022-01-28 2022-05-06 赛纳生物科技(北京)有限公司 一种芯片上恒温扩增测序的方法
WO2023150163A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from lymphatic tissue
WO2023150098A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, kits, compositions, and systems for spatial analysis
WO2023150171A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from glioblastoma samples
WO2023154554A1 (en) * 2022-02-14 2023-08-17 The University Of Chicago Materials and methods for large-scale spatial transcriptomics
WO2023159028A1 (en) 2022-02-15 2023-08-24 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
WO2023172670A2 (en) 2022-03-11 2023-09-14 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
US20230323435A1 (en) * 2022-03-18 2023-10-12 Bio-Rad Laboratories, Inc. Methods and compositions for maximum release of oligonucleotides
WO2023201235A2 (en) 2022-04-12 2023-10-19 10X Genomics, Inc. Compositions and methods for generating and characterizing recombinant antigen binding molecules
WO2023215552A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Molecular barcode readers for analyte detection
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
WO2023229988A1 (en) 2022-05-23 2023-11-30 10X Genomics, Inc. Tissue sample mold
WO2023229982A2 (en) 2022-05-24 2023-11-30 10X Genomics, Inc. Porous structure confinement for convection suppression
WO2023250077A1 (en) 2022-06-22 2023-12-28 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2024015862A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Methods for characterization of antigen-binding molecules from biological samples
WO2024015578A1 (en) 2022-07-15 2024-01-18 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
WO2024031068A1 (en) 2022-08-05 2024-02-08 10X Genomics, Inc. Systems and methods for immunofluorescence quantification
WO2024036191A1 (en) 2022-08-10 2024-02-15 10X Genomics, Inc. Systems and methods for colocalization
WO2024035844A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Methods for reducing capture of analytes
WO2024040068A1 (en) * 2022-08-15 2024-02-22 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids
WO2024044703A1 (en) 2022-08-24 2024-02-29 10X Genomics, Inc. Compositions and methods for antigenic epitope mapping in biological samples
WO2024081212A1 (en) 2022-10-10 2024-04-18 10X Genomics, Inc. In vitro transcription of spatially captured nucleic acids
WO2024081869A1 (en) 2022-10-14 2024-04-18 10X Genomics, Inc. Methods for analysis of biological samples

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985549A (en) * 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
WO1998011210A1 (fr) * 1996-09-13 1998-03-19 Laboratory Of Molecular Biophotonics Phase solide pour la detection d'acides nucleiques cibles, procede de production de cette phase et procede de detection d'acide nucleique cible
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
EP2256133B1 (en) 1997-01-08 2016-12-14 Sigma-Aldrich Co. LLC Bioconjugation of macromolecules
ES2563643T3 (es) 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
EP1975251A3 (en) 2000-07-07 2009-03-25 Visigen Biotechnologies, Inc. Real-time sequence determination
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP2226316B1 (en) 2002-05-30 2016-01-13 The Scripps Research Institute Copper-catalysed ligation of azides and acetylenes
ES2407681T3 (es) 2002-08-23 2013-06-13 Illumina Cambridge Limited Nucleótidos modificados para la secuenciación de polinucleótidos.
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7259258B2 (en) 2003-12-17 2007-08-21 Illumina, Inc. Methods of attaching biological compounds to solid supports using triazine
JP2007525571A (ja) 2004-01-07 2007-09-06 ソレクサ リミテッド 修飾分子アレイ
CA2579150C (en) 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US7393665B2 (en) * 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
EP3373174A1 (en) 2006-03-31 2018-09-12 Illumina, Inc. Systems and devices for sequence by synthesis analysis
WO2008051530A2 (en) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP4134667A1 (en) 2006-12-14 2023-02-15 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
ES2595055T3 (es) * 2009-08-25 2016-12-27 Illumina, Inc. Métodos para seleccionar y amplificar polinucleótidos
DK2556171T3 (da) * 2010-04-05 2015-12-14 Prognosys Biosciences Inc Rumligt kodede biologiske assays
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US9556473B2 (en) 2011-02-15 2017-01-31 Leica Biosystems Newcastle Ltd Methods for identifying nucleic acid sequences
CN102943107A (zh) * 2011-04-08 2013-02-27 蒂莫西·刘 分析生物样本的目标核酸序列的方法
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
SI3623481T1 (sl) 2011-09-23 2022-01-31 Illumina, Inc. Sestavki za sekvenciranje nukleinske kisline
CA2867665C (en) 2012-04-03 2022-01-04 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
EP2647426A1 (en) 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding
WO2014014991A2 (en) * 2012-07-19 2014-01-23 President And Fellows Of Harvard College Methods of storing information using nucleic acids
CA2886974C (en) * 2012-10-17 2021-06-29 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US10086093B2 (en) * 2013-02-28 2018-10-02 The General Hospital Corporation miRNA profiling compositions and methods of use
KR20230074639A (ko) * 2013-08-28 2023-05-30 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
EP3058091B1 (en) * 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
US9834814B2 (en) * 2013-11-22 2017-12-05 Agilent Technologies, Inc. Spatial molecular barcoding of in situ nucleic acids
US9677132B2 (en) * 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
CN104561290A (zh) * 2014-12-30 2015-04-29 东南大学 一种基于混合探针基因芯片检测方法

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