DK3303613T3 - Fremgangsmåde til detektering af aktivitet af en polymerase - Google Patents

Fremgangsmåde til detektering af aktivitet af en polymerase Download PDF

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Publication number
DK3303613T3
DK3303613T3 DK16725866.4T DK16725866T DK3303613T3 DK 3303613 T3 DK3303613 T3 DK 3303613T3 DK 16725866 T DK16725866 T DK 16725866T DK 3303613 T3 DK3303613 T3 DK 3303613T3
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DK
Denmark
Prior art keywords
polymerase
detecting activity
activity
detecting
Prior art date
Application number
DK16725866.4T
Other languages
English (en)
Inventor
Nils-Göran Larsson
Claes Gustafsson
Maria Falkenberg-Gustafsson
Tim Bergbrede
Original Assignee
Max Planck Gesellschaft
Lead Discovery Center Gmbh
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Filing date
Publication date
Application filed by Max Planck Gesellschaft, Lead Discovery Center Gmbh filed Critical Max Planck Gesellschaft
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Publication of DK3303613T3 publication Critical patent/DK3303613T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6825Nucleic acid detection involving sensors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/91Transferases (2.)
    • G01N2333/912Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205Phosphotransferases in general
    • G01N2333/91245Nucleotidyltransferases (2.7.7)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
DK16725866.4T 2015-05-29 2016-05-30 Fremgangsmåde til detektering af aktivitet af en polymerase DK3303613T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15169966.7A EP3098322A1 (en) 2015-05-29 2015-05-29 Method to detect activity of a polymerase
PCT/EP2016/062198 WO2016193231A1 (en) 2015-05-29 2016-05-30 Method to detect activity of a polymerase

Publications (1)

Publication Number Publication Date
DK3303613T3 true DK3303613T3 (da) 2020-03-30

Family

ID=53298173

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16725866.4T DK3303613T3 (da) 2015-05-29 2016-05-30 Fremgangsmåde til detektering af aktivitet af en polymerase

Country Status (4)

Country Link
US (1) US10883137B2 (da)
EP (2) EP3098322A1 (da)
DK (1) DK3303613T3 (da)
WO (1) WO2016193231A1 (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107356572A (zh) * 2017-06-28 2017-11-17 成都理工大学 利用核酸作为载体的荧光共振能量转移方法
EP3684762B1 (en) 2017-09-20 2023-05-03 Lead Discovery Center GmbH Coumarin derivatives, processes for their preparation and uses thereof for the treatment of cancer
CN108796041B (zh) * 2018-06-27 2021-07-27 中南民族大学 一种基于生物发光共振能量转移的信号扩增系统及其检测方法
US10703735B2 (en) 2018-07-25 2020-07-07 Lead Discovery Center Gmbh 4-phenyl-coumarin derivatives, processes for their preparation and uses thereof for the treatment of cancer
WO2020188049A1 (en) 2019-03-20 2020-09-24 Lead Discovery Center Gmbh Quinoline derivatives, processes for their preparation and uses thereof for the treatment of cancer
WO2020252399A1 (en) * 2019-06-14 2020-12-17 University Of Washington An enzymatic assay to measure long-term adherence to pre exposure prophylaxis and antiretroviral therapy
US20240252454A1 (en) 2021-05-03 2024-08-01 Lead Discovery Center Gmbh Composition comprising an inhibitor of mitochondrial transcription
WO2023288319A1 (en) * 2021-07-16 2023-01-19 Ultima Genomics, Inc. Polymerase assay
US20230304086A1 (en) * 2022-03-28 2023-09-28 Illumina Cambridge Limited Labeled avidin and methods for sequencing

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010036359A2 (en) * 2008-09-25 2010-04-01 Allelogic Biosciences Corporation Methods of polymerase activity assay
WO2010129787A2 (en) * 2009-05-08 2010-11-11 The Johns Hopkins University Single molecule spectroscopy for analysis of cell-free nucleic acid biomarkers
US10301674B2 (en) * 2010-08-20 2019-05-28 Life Technologies Corporation Polymerase assay with a FRET substrate

Also Published As

Publication number Publication date
WO2016193231A1 (en) 2016-12-08
EP3303613A1 (en) 2018-04-11
EP3303613B1 (en) 2020-01-08
US10883137B2 (en) 2021-01-05
EP3098322A1 (en) 2016-11-30
US20180142285A1 (en) 2018-05-24

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