DK3177728T3 - Forbedrede fermenteringsfremgangsmåder ved anvendelse af xylanase og pectinase - Google Patents

Forbedrede fermenteringsfremgangsmåder ved anvendelse af xylanase og pectinase Download PDF

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Publication number
DK3177728T3
DK3177728T3 DK15733668.6T DK15733668T DK3177728T3 DK 3177728 T3 DK3177728 T3 DK 3177728T3 DK 15733668 T DK15733668 T DK 15733668T DK 3177728 T3 DK3177728 T3 DK 3177728T3
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Denmark
Prior art keywords
fermentation
acid
starch
ethanol
xylanase
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DK15733668.6T
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English (en)
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DK3177728T4 (da
Inventor
Marco Krämer
Vitaly Svetlitchnyi
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Direvo Ind Biotechnology Gmbh
Basf Enzymes Llc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/14Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015Polygalacturonase (3.2.1.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (12)

1. Fremgangsmåde til fremstilling af et fermenteringsprodukt ud af et stivelsesholdigt materiale, hvilken fremgangsmåde omfatter følgende trin: i) omdannelse af det stivelsesholdige materiale til fermenterbare sukkere, hvor det stivelsesholdige materiale er majs, efterfulgt af ii) fermentering af de fermenterbare sukkere med en mikroorganisme til fermenteret mæsk, iii) udsættelse af fermenteringsmediet under fermenteringsprocessen for en enzymsammensætning, der omfatter en xylanase og en pectinase, iv) fraseparering af fermenteringsproduktet i den fermenterede mæsk.
2. Fremgangsmåde ifølge krav 1, hvor fermenteringsproduktet vælges fra gruppen bestående af en syre, en alkohol og hydrogen.
3. Fremgangsmåde ifølge krav 2, hvor alkoholen vælges fra gruppen bestående af ethanol, butanol, propanol, methanol, propandiol og butandiol.
4. Fremgangsmåde ifølge krav 2, hvor syren vælges fra gruppen bestående af mælkesyre, propionsyre, eddikesyre, succinsyre, æblesyre, smørsyre og myresyre.
5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor mikroorganismen er en bakterie, gær eller svamp.
6. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter (a) flydendegørelse af et stivelsesholdigt materiale med en alpha-amylase og præ-saccharificering af det flydendegjorte materiale før trin (b); (b) saccharificering af det flydendegjorte materiale efterfulgt af (c) fermentering ved anvendelse af en fermenteringsorganisme; hvor en enzymsammensætning, der omfatter en xylanase og en pectinase, tilsættes i fermenteringstrinnet (c).
7. Fremgangsmåde ifølge krav 6, hvor fermenteringsproduktet vælges fra gruppen bestående af en syre, en alkohol og hydrogen.
8. Fremgangsmåde ifølge krav 7, hvor alkoholen vælges fra gruppen bestående af ethanol, butanol, propanol, methanol, propandiol og butandiol.
9. Fremgangsmåde ifølge krav 7, hvor syren vælges fra gruppen bestående af mælkesyre, propionsyre, eddikesyre, succinsyre, æblesyre, smørsyre og myresyre.
10. Fremgangsmåde ifølge et hvilket som helst af kravene 6-9, hvor det stivelsesholdige materiale opnås fra kom og/eller rodknolde.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 6-10, hvor det stivelsesholdige materiale vælges fra gruppen bestående af majs, hvede, byg, mg, hirse, durra og milo.
12. Fremgangsmåde ifølge et hvilket som helst af kravene 6-11, hvor mikroorganismen er en bakterie, gær eller svamp.
DK15733668.6T 2014-08-05 2015-06-24 Forbedrede fermenteringsfremgangsmåder ved anvendelse af xylanase og pectinase DK3177728T4 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462033327P 2014-08-05 2014-08-05
EP14179851 2014-08-05
PCT/EP2015/064179 WO2016020103A1 (en) 2014-08-05 2015-06-24 Improved fermentation processes

Publications (2)

Publication Number Publication Date
DK3177728T3 true DK3177728T3 (da) 2018-12-17
DK3177728T4 DK3177728T4 (da) 2022-02-28

Family

ID=51263314

Family Applications (2)

Application Number Title Priority Date Filing Date
DK15733668.6T DK3177728T4 (da) 2014-08-05 2015-06-24 Forbedrede fermenteringsfremgangsmåder ved anvendelse af xylanase og pectinase
DK15748032.8T DK3177729T3 (da) 2014-08-05 2015-08-05 Forbedret batch-tid i fermenteringsprocesser ved anvendelse af xylanase og pectinase

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK15748032.8T DK3177729T3 (da) 2014-08-05 2015-08-05 Forbedret batch-tid i fermenteringsprocesser ved anvendelse af xylanase og pectinase

Country Status (6)

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US (2) US20170226540A1 (da)
EP (2) EP3177728B2 (da)
DK (2) DK3177728T4 (da)
ES (2) ES2699845T5 (da)
PL (2) PL3177728T5 (da)
WO (2) WO2016020103A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017067698A1 (en) * 2015-10-22 2017-04-27 Direvo Industrial Biotechnology Gmbh Method for producing a fermentation product
EP3530743A1 (en) 2018-02-21 2019-08-28 Cambridge Glycoscience Ltd Method of production
BR112021002910A2 (pt) 2018-08-15 2021-07-20 Cambridge Glycoscience Ltd novas composições, seu uso e métodos para sua formação
JP2022545650A (ja) 2019-08-16 2022-10-28 ケンブリッジ グリコサイエンス エルティーディー バイオマスを処理してオリゴ糖および関連組成物を生成する方法
JP2023506464A (ja) 2019-12-12 2023-02-16 ケンブリッジ グリコサイエンス エルティーディー 低糖の多相食料品

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4226528A1 (de) 1992-08-11 1994-02-17 Roehm Gmbh Batterien-Xylanase, Verfahren zu ihrer Herstellung sowie dafür geeigneter Bakterienstamm, Plasmid mit zugehörigem Strukturgen, sowie Backmittel und Backverfahren zur Herstellung von Brot und Backwaren unter Verwendung der Xylanase
DE69435154D1 (de) 1993-03-10 2008-12-04 Novozymes As Enzyme mit Xylanaseaktivität aus Aspergillus aculeatus
AU2002210409A1 (en) * 2000-11-10 2002-05-21 Novozymes A/S Ethanol process
CN101389645B (zh) 2004-02-12 2016-08-03 诺维信股份有限公司 具有木聚糖酶活性的多肽和编码它的多核苷酸
US7851193B2 (en) 2007-12-05 2010-12-14 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
CN101892269A (zh) 2009-05-21 2010-11-24 中国科学院成都生物研究所 以甘薯为原料发酵生产高浓度乙醇的方法
DK2655644T3 (da) 2010-12-22 2019-03-04 Direvo Ind Biotechnology Gmbh Forbedring af fermenteringsprocesser og biprodukter
ES2709102T3 (es) * 2013-02-21 2019-04-15 Direvo Ind Biotechnology Gmbh Ligantes de micotoxinas

Also Published As

Publication number Publication date
PL3177728T5 (pl) 2022-03-21
ES2699845T5 (es) 2022-04-11
US20170233768A1 (en) 2017-08-17
US10323260B2 (en) 2019-06-18
DK3177729T3 (da) 2018-12-17
EP3177728B1 (en) 2018-10-17
EP3177728B2 (en) 2021-12-01
EP3177729A1 (en) 2017-06-14
US20170226540A1 (en) 2017-08-10
WO2016020468A1 (en) 2016-02-11
EP3177729B1 (en) 2018-10-10
PL3177728T3 (pl) 2019-05-31
ES2699845T3 (es) 2019-02-13
EP3177728A1 (en) 2017-06-14
ES2699677T3 (es) 2019-02-12
DK3177728T4 (da) 2022-02-28
WO2016020103A1 (en) 2016-02-11
PL3177729T3 (pl) 2019-04-30

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