DK3152573T3 - Fremgangsmåde til analytisk autenticitetskontrol af indholdsstoffer i levnedsmidler under anvendelse af kunstige antistoffer - Google Patents

Fremgangsmåde til analytisk autenticitetskontrol af indholdsstoffer i levnedsmidler under anvendelse af kunstige antistoffer Download PDF

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DK3152573T3
DK3152573T3 DK15747363.8T DK15747363T DK3152573T3 DK 3152573 T3 DK3152573 T3 DK 3152573T3 DK 15747363 T DK15747363 T DK 15747363T DK 3152573 T3 DK3152573 T3 DK 3152573T3
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acid
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food
ethyl
methyl
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Mikko Hofsommer
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Gfl Ges Fuer Lebensmittel Forschung Mbh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
    • B01J20/267—Cross-linked polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/268—Polymers created by use of a template, e.g. molecularly imprinted polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2600/00—Assays involving molecular imprinted polymers/polymers created around a molecular template

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Claims (12)

1. Fremgangsmåde til autenticitetskontrol af en analyt fra en fødevare, kendetegnet ved, at a) ekstraktionen af den ønskede anlyt fra fødevaren eller et fødevareekstrakt finder sted ved molecularly imprinted polymers (MIPs), at b) MlPS'erne ladet med analyten udskilles, at c) fra MlP'erne ladet med analyten fjernes analyten igen og at d) den således ekstraherede analyt underkastes en isotop-forhold-masse-spektroskopi (IRMS) eller en cavity-ring-down-spektroskopi (CRDS), for bestemmelse af isotopforholdet valgt blandt 1H/2H, 13C/12C, 16O/17O/18O og/eller 15N/14N.
2. Fremgangsmåde ifølge krav 1, kendetegnet ved, at ekstraktionen af analyten finder sted således, at MlP'erne er placerede i en patron.
3. Fremgangsmåde ifølge krav 1, kendetegnet ved, at analyten er saccharose, glucose, fructose, maltose, sorbitol, vanillin, benzaldehyd, ethylbutyrat, gamma-octalacton, delta-decalacton, gamma-decalacton, trans-2-hexenal, 1-hexanol, trans-2-hexanol, hexylacetat, ethyl-3-(methylthio)-propionat, methyl-3-(methylthio)-propionat, alpha-ionon, ethyl-2,4-decadienoat, hexylhexanoat, nootkaton, decanal, furaneol-(4-hydroxy-2,5-dimethyl-3(2H)furanon), mesifuran(2,5-dimethyl-4-methoxy-3(2H)-furanon), ascorbinsyre, dehydroascorbinsyre, malinsyre, æblesyre, isocitronsyre, vinsyre, aminosyrer, glycerol, phosphae, caffein eller et derivat af disse substanser.
4. Fremgangsmåde ifølge krav 3, kendetegnet ved, at analyten er vanillin, ascorbinsyre, saccharose, glucose, fructose eller et derivat af disse substanser.
5. Fremgangsmåde ifølge krav 1, kendetegnet ved, at fødevaren er valgt fra følgende liste: frugt/grøntsagsjuice og produkter tilvejebragt deraf, sodavand, alkoholiske drikkevarer, vin, spiritus, Øl, sukkervarer, chokolade, honning, iscreme, marmelade, babymad, fisk/kød og produkter tilvejebragt deraf.
6. Fremgangsmåde ifølge krav 5, kendetegnet ved, at fødevaren er frugt/grønt-sagsjuice eller et produkt tilvejebragt deraf.
7. Anvendelse af en molecularly imprinted polymer (MIP), omfattende en polymer, som er tilvejebragt under anvendelse af en fødevareingrediens som et molekylært mål, idet enhver af følgende substanser acrylsyre, methacrylsyre, methylmethacrylat, 1-vinylimidazol, 4(5)-vinylimidazol, 2-vinylpyridin, itaconsyre, p-vinylbenzoesyre, ethylstyren, (2-methacrylamido)-6-methylpyridin, 2-acrylamido-2-methyl-1-propansulfonsyre, p-vinylphenylborsyre, p-vinylbenzylamin, 2-(p-vinylphenyl)-1,3-propandiol, p-vinylbenzylalkohol, p-vinylphenylcarbonyl, p-vinylbenzoesyre anvendes som en monomer, og i det mindste én af følgende substanser ethylenglycoldimethacrylat (EGDMA), paradivinylbenzen (p-DVB), N,N'-methylen-diacrylamid, N,N'-1,4-phenylendiacrylamid, N,O-bis-acrylol-L-phenylalaninol, tri-methylolpropantrimethacrylat (TRIM), pentaerythritoltriacrylat og pentaerythritoltetraacrylat anvendes som en tværbinder, kendetegnet ved, at MlP'en selektivt binder til den oprindeligt anvendte fødevareingrediens som et molekylært mål, for udøvelse af en fremgangsmåde til bestemmelse af autenticiteten af en analyt fra en fødevare ifølge krav 1.
8. Anvendelse af en molecularly imprinted polymer (MIP) ifølge krav 7, kendetegnet ved, at i det mindste to af følgende substanser acrylsyre, methacrylsyre, methylmethacrylat, 1-vinylimidazol, 4(5)-vinylimidazol, 2-vinylpyridin, itaconsyre, p-vinyl-benzoesyre, ethylstyren, (2-methacrylamido)-6-methylpyridin, 2-acrylamido-2-methyl-1-propansulfonsyre, p-vinylphenylborsyre, p-vinylbenzylamin, 2-(p-vinylphenyl)-1,3- propandiol, p-vinylbenzylalkohol, p-vinylphenylcarbonyl, p-vinylbenzoesyre, anvendes som monomerer.
9. Anvendelse af en molecularly imprinted polymer (MIP) ifølge krav 7, kendetegnet ved, at målet er saccharose, glucose eller fructose, maltose, sorbitol, vanillin, benz-aldehyd, ethylbutyrat, gamma-octalacton, delta-decalacton, gamma-decalacton, trans-2-hexenal, 1-hexanol, trans-2-hexanol, hexylacetat, ethyl-3-(methylthio)-propionat, methyl-3-(methylthio)-propionat, alpha-ionon, ethyl-2,4-decadienoat, hexylhexanoat, nootkaton, decanal, furanol(4-hydroxy-2,5-dimethyl-3(2H)furanon), mesifuran(2,5-dimethyl-4-methoxy-3(2H)furanon), ascorbinsyre, dehydroascorbinsyre, æblesyre, citronsyre, isocitronsyre, vinsyre, aminosyrer, glycerol, phosphat, coffein, eller foreligger i en derivatiseret form.
10. På forhånd fyldt patron, omfattende i det mindste én molecularly imprinted polymer (MIP), omfattende en polymer, som er tilvejebragt under anvendelse af en fødevareingrediens som et molekylært mål, idet i det mindste én af følgende substanser, acryl-syre, methacrylsyre, methylmethacrylat, 1-vinylimidazol, 4(5)-vinylimidazol, 2-vinyl-pyridin, itaconsyre, p-vinylbenzoesyre, ethylstyren, (2-methacrylamido)-6-methyl-pyridin, 2-acrylamido-2-methyl-1-propansulfonsyre, p-vinylphenylborsyre, p-vinyl-benzylamin, 2-(p-vinylphenyl)-1,3-propandiol, p-vinylbenzylalkohol p-vinylphenyl-carbonyl, p-vinylbenzoesyre, anvendes som en monomer, og i det mindste én af følgende substanser ethylenglycoldimethacrylat (EGDMA), paradivinylbenzen (p-DVB), N,N'-methylen-diacrylamid, N,N'-1,4-phenylendiacrylamid, N,O-bis-acrylol-L-phenylalaninol, trimethylolpropantrimethacrylat (TRIM), pentaerythritoltriacrylat og pentaerythritol-tetraacrylat anvendes som en tværbinder, hvor MlP'en selektivt binder til den oprindelige fødevareingrediens som et molekylært mål, til anvendelse ved en fremgangsmåde til autentitetskontrol af en analyt fra en fødevare ved isotop-forhold-masse-spektroskopi (IRMS) eller cavit-ring-down-spektroskopi (CRDS), for bestemmelse af isotopforholdet, valgt blandt 1H/2H, 13C/12C, 16O/17O/18O og/eller 15N/14N.
11. På forhånd fyldt patron ifølge krav 10, kendetegnet ved, at i det mindste to af følgende substanser acrylsyre, methacrylsyre, methylmethacrylat, 1-vinylimidazol, 4(5)-vinylimidazol, 2-vinylpyridin, itaconsyre, p-vinylbenzoesyre, ethylstyren, (2-methacrylamido)-6- methylpyridin, 2-acrylamido-2-methyl-1-propansulfonsyre, p-vinylphenylborsyre, p-vinylbenzylamin, 2-(p-vinylphenyl)-1,3-propandiol, p-vinylbenzylalkohol, p-vinyl-phenylcarbonyl, p-vinylbenzoesyre, er blevet anvendt som monomerer for fremstillingen af MlP'en.
12. På forhånd fyldt patron ifølge krav 10, kendetegnet ved, at målet er saccharose, glucose eller fructose, maltose, sorbitol, vanillin, benzaldehyd, ethylbutyrat, gamma-octalacton, delta-decalacton, gamma-decalacton, trans-2-hexenal, 1-hexanol, trans-2-hexanol, hexylacetat, ethyl 3-(methylthio)-propionat, methyl 3-(methylthio)-propionat, alpha-ionon, ethyl-2,4-decadienoat, hexylhexanoat, nootkaton, decanal, furanol(4-hydroxy-2,5-dimethyl-3(2H)furanon), mesifuran(2,5-dimethyl-4-methoxy-3(2H)-furanon), ascorbinsyre, dehydroascorbinsyre, æblesyre, citrronsyre, isocitronsyre, vinsyre, aminosyrer, glycerol, phosphat, coffein, eller foreligger i en derivatiseret form.
DK15747363.8T 2014-06-06 2015-06-08 Fremgangsmåde til analytisk autenticitetskontrol af indholdsstoffer i levnedsmidler under anvendelse af kunstige antistoffer DK3152573T3 (da)

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DE102014008135.4A DE102014008135A1 (de) 2014-06-06 2014-06-06 Verfahren zur analytischen Authentizitätprüfung von Inhaltsstoffen in Lebensmitteln unter Verwendung künstlicher Antikörper
PCT/DE2015/000278 WO2015185030A1 (de) 2014-06-06 2015-06-08 Verfahren zur analytischen authentizitätsprüfung von inhaltsstoffen in lebensmitteln unter verwendung künstlicher antikörper

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DE102016212460A1 (de) * 2016-07-08 2018-01-11 Robert Bosch Gmbh Verbundmaterial, Anordnung und Verfahren zur Festphasenextraktion von flüssigen Lebensmitteln
CN107300565B (zh) * 2017-08-25 2019-03-19 江苏出入境检验检疫局动植物与食品检测中心 一种快速鉴别蜂蜜中掺入大米糖浆的核磁共振波谱方法
CN108802222B (zh) * 2018-06-13 2021-08-06 中国农业科学院蜜蜂研究所 一种基于挥发性物质鉴别冬蜜和乌桕蜜的方法
CN114324650B (zh) * 2021-12-27 2023-10-13 南京天嘉检测服务有限公司 一种基于（z）-edto特征标志物鉴别九龙藤蜂蜜的方法

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SE9203435D0 (sv) 1992-11-11 1992-11-11 Georg Vlatakis Fondation For R Framstaellande av artificiella antikroppar med molekylavtrycksmetoden samt deras anvaendning vid analys
US6274686B1 (en) 1997-05-30 2001-08-14 Klaus Mosbach Amide containing molecular imprinted polymers
AU2003242600A1 (en) * 2002-05-31 2003-12-19 Austria Wirtschaftsservice Gesellschaft Mit Beschrankter Haftung Solid mycoctoxin carriers
JP5468736B2 (ja) * 2004-05-24 2014-04-09 ブリティッシュ アメリカン タバコ （インヴェストメンツ） リミテッド ニトロサミン類に選択的なモレキュラーインプリントポリマーおよびその使用方法
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