DK3102637T3 - Fremgangsmåde til oprensning af antistoffer, antistoffragmenter eller manipulerede varianter deraf ved anvendelse af specifikke anthraquinonfarvestofligand-strukturer - Google Patents

Fremgangsmåde til oprensning af antistoffer, antistoffragmenter eller manipulerede varianter deraf ved anvendelse af specifikke anthraquinonfarvestofligand-strukturer Download PDF

Info

Publication number
DK3102637T3
DK3102637T3 DK15704492.6T DK15704492T DK3102637T3 DK 3102637 T3 DK3102637 T3 DK 3102637T3 DK 15704492 T DK15704492 T DK 15704492T DK 3102637 T3 DK3102637 T3 DK 3102637T3
Authority
DK
Denmark
Prior art keywords
absorbent
alkyl
dye
formula
adsorbent
Prior art date
Application number
DK15704492.6T
Other languages
English (en)
Inventor
Max Fabian Felle
Mari Granström
David Linder
Shilpa S Korde
Amarish Samel
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP14159349.1A external-priority patent/EP2918641A1/en
Application filed by Basf Se filed Critical Basf Se
Priority claimed from IN324DE2014 external-priority patent/IN2014DE00324A/en
Application granted granted Critical
Publication of DK3102637T3 publication Critical patent/DK3102637T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/288—Polar phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/3085—Chemical treatments not covered by groups B01J20/3007 - B01J20/3078
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/321—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3257—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one of the heteroatoms nitrogen, oxygen or sulfur together with at least one silicon atom, these atoms not being part of the carrier as such
    • B01J20/3261—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one of the heteroatoms nitrogen, oxygen or sulfur together with at least one silicon atom, these atoms not being part of the carrier as such comprising a cyclic structure not containing any of the heteroatoms nitrogen, oxygen or sulfur, e.g. aromatic structures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
    • C09B67/0097—Dye preparations of special physical nature; Tablets, films, extrusion, microcapsules, sheets, pads, bags with dyes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B69/00—Dyes not provided for by a single group of this subclass
    • C09B69/10—Polymeric dyes; Reaction products of dyes with monomers or with macromolecular compounds
    • C09B69/101—Polymeric dyes; Reaction products of dyes with monomers or with macromolecular compounds containing an anthracene dye
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/40—Aspects relating to the composition of sorbent or filter aid materials
    • B01J2220/49—Materials comprising an indicator, e.g. colour indicator, pH-indicator

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Inorganic Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)

Claims (15)

1. Adsorptionsmiddel til kromatografisk oprensing af immunoglobulinmolekyler, hvilket absorptionsmiddel omfatter en bærematrix, hvortil en farvestofligand er kovalent bundet, hvor farvestofliganden er valgt blandt anthraquinonfarvestoffer, og hvor liganddensiteten på absorptionsmidlet er mere end 150 pmol/g tørt absorptionsmiddel.
2. Absorptionsmiddel ifølge krav 1, hvor liganddensiteten på absorptionsmidlet er 160 til 500 pmol/g tørt absorptionsmiddel, fortrinsvis 200 til 350 pmol/g tørt absorptionsmiddel.
3. Absorptionsmiddel ifølge krav 1 eller 2, hvor absorptionsmidlet omfatter reaktionsproduktet af bærematrixen, eventuelt et linkermolekyle og en anthraquinonforbindelse med den generelle formel 1
(1) hvor X er halogen; k og m uafhængigt af hinanden er 0 eller 1, og summen af k+m er 1 eller 2; B er et aromatisk radikal, der omfatter en eller flere, kondenserede eller ikke-kondenserede, eventuelt mono- eller polysubstituerede aromatiske ringe; V er en substituent med formlen
(2a) eller
(2b) hvor n hver er O eller 1; med det forbehold at begge n ikke samtidigt er 0; R er ens eller forskellige og valgt blandt H, alkyl, NH2, NH-alkyl, NH-aryl, N(alkyl)2, NO2, OH, O-alkyl, CN, C(O)alkyl, C(O)aryl, C(O)NH2, C (O) N (H) alkyl, C (O) N (alkyl) 2, C (O) N (H) aryl, halogen; Ri er hydrogen eller eventuelt substitueret Ci-C4alkyl; Z er valgt blandt en kemisk binding, - (CH2)ni-, hvor ni er et helt tal fra 1 til 4, arylen, -CH2-arylen-, -SO2-arylen-, -C(O)N(H)arylen-, -SO2N(H)arylen-, -CH=CH-, -SO2-CH2-CH2-; -C (O) NH-(CH2) n2~, hvor n2 er et helt tal fra 1 til 4; og Y er ens eller forskellige polære funktionelle grupper valgt blandt -SO3H, -OSO3H, -CO2H, -P(O)(OH)2, -OP (O) (OH) 2, OH og SH.
4. Absorptionsmiddel ifølge et hvilket som helst af ovennævnte krav, hvor B er en substituent med formlen
Y er som defineret ovenfor, n er O eller 1, n3 er O, 1, 2, 3 eller 4, Yi er ens eller forskellige og valgt blandt halogen, alkyl, alkoxy, NO2 eller Y, hvor Y er som defineret ovenfor; W er en kemisk binding eller valgt blandt -C(O)N(H)-, -SO2N(H)-, -SO2- eller -N(H)-; og R2 er valgt blandt H eller alkyl.
5. Absorptionsmiddel ifølge et hvilket som helst af ovennævnte krav, hvor B er et aromatisk radikal med formel 3a, og/eller hvor V er en substituent til formel 2a.
6. Absorptionsmiddel ifølge krav 5, hvor B er et aromatisk radikal med formel 3a, hvor n3 er 0, 1, 2, 3 eller 4, og Yi uafhængigt er alkyl eller Y.
7. Absorptionsmiddel ifølge krav 5, hvor V er en substituent til formel 2a, hvor R er H, og Z er valgt blandt en kemisk binding eller -SO2-CH2-CH2-.
8. Absorptionsmiddel ifølge et hvilket som helst af ovennævnte krav, hvor k er 0, og m er 1.
9. Absorptionsmiddel ifølge et hvilket som helst af kravene 1 til 8, der har en gennemsnitlig partikelstørrelse i intervallet fra 10 til 200, såsom 20 til 180 eller 45 til 165 eller 80 til 120 eller 90 til 110 pm.
10. Fremgangsmåde til kromatografisk oprensning af immunoglobulinmolekyler, hvilken fremgangsmåde omfatter etablering af kontakt mellem en prøve, der indeholder mindst én ønsket type af immunoglobulinmolekyler i en blanding med kontaminerende uorganiske og/eller organiske stoffer, og et absorptionsmiddel ifølge et hvilket som helst af kravene 1 til 9, adsorbering af den ønskede type af immunoglobulinmolekyler på absorptionsmidlet, eventuelt vask af absorptionsmidlet med en vaskevæske og efterfølgende eluering af det adsorberede materiale til opnåelse af et eluat, der indeholder de ønskede immunoglobulinmolekyler i beriget form; eller hvilken fremgangsmåde omfatter etablering af kontakt mellem en prøve, der indeholder mindst én ønsket type af immunoglobulinmolekyler i en blanding med kontaminerende uorganiske og/eller organiske stoffer, og et absorptionsmiddel ifølge et hvilket som helst af kravene 1 til 9, adsorbering af de kontaminerende uorganiske og/eller organiske stoffer, opnåelse af et gennemstrømningsmateriale, der indeholder de ønskede immunoglobulinmolekyler i en mere ren form.
11. Fremgangsmåde ifølge krav 10, hvor den ønskede type af immunoglobulinmolekyle er et funktionelt antistof (Ab) eller fragmentantistof (fAb).
12. Fremgangsmåde ifølge et af kravene 10 og 11, hvor den immunoglobulinholdige prøve, der skal oprenses, er kontamineret med denaturerede og/eller agglomererede/multimeriserede immunoglobuliner.
13. Anvendelse af en forbindelse ifølge et hvilket som helst af kravene 1 til 8 til separering af immunoglobulinmolekyler, især Ab'er og fAb'er.
14. Præparativt eller analytisk kit, der omfatter mindst ét absorptionsmiddel, der bærer en anthraquinonforbindelse ifølge et hvilket som helst af kravene 1 til 9, og yderligere materiale til identificering af mindst ét separeret fAb.
15. Fremgangsmåde til fremstilling af et absorptionsmiddel ifølge et af kravene 1 til 9, hvilken fremgangsmåde omfatter reaktion af et eventuelt aktiveret bæremiddel med en egnet linker som for eksempel tri-2'-aminoethylamin (TREN), efterfulgt af reaktion af det således opnåede funktionaliserede absorptionsmiddel med en anthraquinonforbindelse ifølge krav 1 til 8.
DK15704492.6T 2014-02-04 2015-02-04 Fremgangsmåde til oprensning af antistoffer, antistoffragmenter eller manipulerede varianter deraf ved anvendelse af specifikke anthraquinonfarvestofligand-strukturer DK3102637T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14159349.1A EP2918641A1 (en) 2014-03-13 2014-03-13 Method for purification of antibodies, antibody fragments or engineered variants thereof using specific anthraquinone dye-ligand structures
PCT/EP2015/052246 WO2015117984A1 (en) 2014-02-04 2015-02-04 Method for purification of antibodies, antibody fragments or engineered variants thereof using specific anthraquinone dye-ligand structures
IN324DE2014 IN2014DE00324A (da) 2014-02-04 2015-02-04

Publications (1)

Publication Number Publication Date
DK3102637T3 true DK3102637T3 (da) 2018-07-23

Family

ID=52472296

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15704492.6T DK3102637T3 (da) 2014-02-04 2015-02-04 Fremgangsmåde til oprensning af antistoffer, antistoffragmenter eller manipulerede varianter deraf ved anvendelse af specifikke anthraquinonfarvestofligand-strukturer

Country Status (9)

Country Link
US (1) US20160347789A1 (da)
EP (1) EP3102637B1 (da)
JP (1) JP2017512131A (da)
KR (1) KR20160119134A (da)
CN (1) CN105980485B (da)
DK (1) DK3102637T3 (da)
IL (1) IL246881B (da)
SG (1) SG11201605954SA (da)
WO (1) WO2015117984A1 (da)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6564539B1 (ja) 2018-09-14 2019-08-21 長瀬産業株式会社 スルホン酸化合物によるペプチド精製方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4016149A (en) * 1973-09-10 1977-04-05 Research Corporation Selective removal of albumin from blood fluid and compositions therefore
US4043997A (en) * 1976-05-28 1977-08-23 Cutter Laboratories, Inc. Method for isolating albumin using insoluble supports coupled to a dye
JPS635133B2 (da) * 1978-01-24 1988-02-02 Paburitsuku Herusu Raboratarii Saauisu Boodo
ES2332893T3 (es) * 1996-08-30 2010-02-15 Upfront Chromatography A/S Aislamiento de inmunoglobulinas.
AU2002951240A0 (en) * 2002-08-23 2002-09-19 Royal Women's Hospital Depletion of plasma proteins
BRPI0514435B8 (pt) * 2004-08-20 2021-05-25 American Nat Red Cross isolamento sequêncial de proteínas e esquemas de purificação através de cromatografia por afinidade
MY144344A (en) * 2005-04-08 2011-09-15 Ciba Holding Inc Adsorbents comprising anthraquinone dye-ligands for the separation of biological materials
SG144809A1 (en) * 2007-01-11 2008-08-28 Millipore U K Ltd Benzimidazole compounds and their use as chromatographic ligands
JP2011500757A (ja) * 2007-10-22 2011-01-06 メルク セローノ ソシエテ アノニム Ｆｃ含有タンパク質の精製方法
US9127043B2 (en) * 2009-03-05 2015-09-08 Biogen Ma Inc. Purification of immunoglobulins
WO2011044637A1 (en) * 2009-10-15 2011-04-21 Monash University Affinity ligands and methods for protein purification
GB201002824D0 (en) * 2010-02-19 2010-04-07 Temasek Polytechnic A method of preparing a substrate for immobilization of functional substances thereon and the substrate obtained therefrom
CN103732253A (zh) * 2011-08-19 2014-04-16 Emd密理博公司 小分子在纯化生物分子的方法中的用途

Also Published As

Publication number Publication date
JP2017512131A (ja) 2017-05-18
WO2015117984A1 (en) 2015-08-13
KR20160119134A (ko) 2016-10-12
EP3102637A1 (en) 2016-12-14
CN105980485B (zh) 2018-11-27
US20160347789A1 (en) 2016-12-01
EP3102637B1 (en) 2018-04-11
IL246881B (en) 2018-03-29
CN105980485A (zh) 2016-09-28
SG11201605954SA (en) 2016-08-30

Similar Documents

Publication Publication Date Title
KR101149969B1 (ko) 항체 정제
TWI570134B (zh) 包含新穎之基於金黃色葡萄球菌蛋白質ａ之配位子的層析基質
AU2009238686B2 (en) Chromatography medium
JP5917417B2 (ja) 抗体の選択的強化
BRPI0518207B1 (pt) Método de separação de um ou mais anticorpos de um ou mais outros compostos em uma amostra líquida
Moczko et al. Epitope approach in molecular imprinting of antibodies
EP2970379B1 (en) Methods of increasing protein purity using protein a based chromatography
AU2010306415A1 (en) Affinity ligands and methods for protein purification
CN104812491B (zh) 阴离子交换-疏水性混合模式
Guo et al. Synthesis of nanoparticles with a combination of metal chelation and molecular imprinting for efficient and selective extraction of glycoprotein
DK3102637T3 (da) Fremgangsmåde til oprensning af antistoffer, antistoffragmenter eller manipulerede varianter deraf ved anvendelse af specifikke anthraquinonfarvestofligand-strukturer
EP2918641A1 (en) Method for purification of antibodies, antibody fragments or engineered variants thereof using specific anthraquinone dye-ligand structures
Sakhnini et al. Designing monoclonal antibody fragment-based affinity resins with high binding capacity by thiol-directed immobilisation and optimisation of pore/ligand size ratio
Fan et al. Facile preparation of salt-tolerant anion-exchange membrane adsorber using hydrophobic membrane as substrate
WO2019244961A1 (ja) 変異ｖｈｈ抗体を用いたアフィニティー担体
JP5737817B2 (ja) 免疫グロブリン凝集体の除去
US20210017223A1 (en) Separation Method
EP4323106A1 (en) Scaffold for isolation of a biomolecule
CN117580850A (zh) 分离双特异性抗体的方法