DK3023151T3 - Fremgangsmåde til fremkaldelse af et lokaliseret cirkulationsområde for fluidumstrømning og tilsvarende pipette - Google Patents

Fremgangsmåde til fremkaldelse af et lokaliseret cirkulationsområde for fluidumstrømning og tilsvarende pipette Download PDF

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DK3023151T3
DK3023151T3 DK15199422.5T DK15199422T DK3023151T3 DK 3023151 T3 DK3023151 T3 DK 3023151T3 DK 15199422 T DK15199422 T DK 15199422T DK 3023151 T3 DK3023151 T3 DK 3023151T3
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pipette
approx
microfluidic
outlet
channels
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DK15199422.5T
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English (en)
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Owe Orwar
Alar Ainla
Aldo Jesorka
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Flucell Ab
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022—Capillary pipettes, i.e. having very small bore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0694—Creating chemical gradients in a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Claims (15)

1. Fremgangsmåde til fremkaldelse af et lokaliseret cirkulationsområde for fluidumstrømning (2508), hvilken fremgangsmåde omfatter: tilvejebringelse af en fritstående mikrofluidikpipette (2500), der omfatter tre eller flere kanaler (2504, 2506) med udgange, som er placeret i et side-om-side arrangement, hvori en udløbskanaludgang (2504) er placeret mellem to indløbskanaludgange (2506), og udgangene er adskilt fra hinanden ved en ydre overflade af pipetten (2500), og styring af fluidumstrømninger gennem kanalerne for at fremkalde den lokaliserede recirkulerende fluidumstrømningsbane uden for pipetten (2500) og tilstødende objektet af interesse i et åbent volumen, hvori den væske, der forlader mikrofluidikpipetten gennem i det mindste én udløbskanaludgang (2504), kommer i kontakt med objektet af interesse, inden det cirkulerer tilbage og trækkes tilbage ind i de to indløbskanaludgange (2506), som er tilstødende den mindst ene udløbskanaludgang (2504) på mikrofluidikpipetten.
2. Fremgangsmåde ifølge krav 1, hvorved den fritstående mikrofluidikpipette (2500) desuden omfatter et fluidumomskiftningspunkt i forbindelse med udløbskanalen (2504), idet omskiftningspunktet er tilpasset og konfigureret til at skifte om mellem en flerhed af fluida.
3. Fremgangsmåde ifølge krav 1, hvorved kanalerne (2504, 2506) er parallelle med et plan, der afgrænses af en bundflade af mikrof luidikpipetten (2500), og en afstand fra én eller flere udgange til planet er på mellem ca. 0,5 og ca. 2 gange højden af den ene eller de flere udgange.
4. Fremgangsmåde ifølge krav 1, hvorved udgangene adskilles fra hinanden med en afstand på mellem ca. 0,5 og ca. 2 gange en største tværsnitsdimension af den ene eller de flere udløbskanaler (2504).
5. Fremgangsmåde ifølge krav 1, hvorved strømningscirkulationen drives af et eller flere fra den gruppe, der består af: tryk, vakuum og en kombination deraf.
6. Fremgangsmåde ifølge krav 1, hvorved objektet af interesse er valgt fra den gruppe, der består af: sensorer, enkeltceller, enkeltcellede mikroorganismer, cellenetværk, biologisk vævskultur, netværk af neurale celler og kombinationer deraf.
7. System, der er tilpasset og konfigureret til at fremkalde et lokaliseret strømningscirkulationsområde (2508), hvilket system omfatter: en fritstående mikrofluidikpipette (2500), der omfatter tre eller flere kanaler med udgange, som er placeret i et side-omside arrangement, hvori en udløbskanaludgang (2504) er placeret mellem to eller flere indløbskanaludgange (2506), og udgangene er adskilt fra hinanden ved en ydre overflade af pipetten (2500) og en positioneringsanordning, og en styreanordning til styring af fluidumstrømninger gennem kanalerne (2504, 2506) for at fremkalde den lokaliserede recirkulerende fluidumstrømningsbane uden for pipetten og tilstødende objektet af interesse i et åbent volumen, hvori den væske, der forlader mikrofluidikpipetten (2500) gennem i det mindste én udløbskanaludgang (2504), trækkes tilbage ind i de to eller flere indløbskanaludgange (2506), som er tilstødende den mindst ene udløbskanaludgang (2504) på mikrofluidikpipetten.
8. System ifølge krav 7, hvori de tre eller flere kanaler (2504, 2506) har en største tværsnitsdimension, som er valgt fra den gruppe, der består af: mellem ca. 5 pm og ca. 10 pm, mellem ca. 10 pm og ca. 15 pm, mellem ca. 15 pm og ca. 20 pm, mellem ca. 20 pm og ca. 25 pm, mellem ca. 25 pm og ca. 30 pm, mellem ca. 30 pm og ca. 35 pm, mellem ca. 35 pm og ca. 40 pm, mellem ca. 40 pm og ca. 45 pm og mellem ca. 45 pm og ca. 50 pm.
9. System ifølge krav 7, hvori den fritstående mikrofluidikpipette (2500) desuden afgrænser én eller flere reservoirer i fluidummæssig forbindelse med den mindst ene udløbskanal.
10. System ifølge krav 7, hvori kanalerne (2504, 2506) er parallelle med et plan, der afgrænses af en bundflade af mikrofluidikpipetten, og en afstand fra én eller flere udgange til planet er på mellem ca. 0,5 og ca. 2 gange højden af den ene eller de flere udgange.
11. System ifølge krav 7, hvori udgangene er adskilt fra hinanden med en afstand på mellem ca. 0,5 og ca. 2 gange en størst tværsnitsdimension af den ene eller de flere udløbskanaler.
12. System ifølge krav 7, hvori positioneringsanordningen er konfigureret til at holde mikrofluidikpipetten i en vinkel på mellem 5° og 45° fra en vandret akse.
13. System ifølge krav 7, hvori den fritstående mikrofluidikpipette (2500) desuden omfatter et fluidumsomskiftningspunkt i forbindelse med udløbskanalen (2504), idet omskiftningspunktet er tilpasset og konfigureret til at skifte om mellem en flerhed af fluida.
14. System ifølge krav 7, hvori den fritstående mikrofluidikpipette (2500) er fremstillet af en elastisk polymer.
15. System ifølge krav 7, hvori den fritstående mikrofluidikpipette (2500) er optisk gennemsigtigt.
DK15199422.5T 2009-12-03 2010-12-03 Fremgangsmåde til fremkaldelse af et lokaliseret cirkulationsområde for fluidumstrømning og tilsvarende pipette DK3023151T3 (da)

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US26625509P 2009-12-03 2009-12-03
EP10821464A EP2506976A2 (en) 2009-12-03 2010-12-03 Pipettes, methods of use, and methods of stimulating an object of interest

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EP3023151B1 (en) 2018-11-07
WO2011067670A2 (en) 2011-06-09
US9671366B2 (en) 2017-06-06
US20150346147A1 (en) 2015-12-03
US20130017553A1 (en) 2013-01-17
EP2506976A2 (en) 2012-10-10
EP3023151A1 (en) 2016-05-25
US9126197B2 (en) 2015-09-08
WO2011067670A3 (en) 2011-12-01

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