DK2981546T3 - Filamentøs svampecelle med inaktiveret komponent fra den selektive autofagisignalvej og fremgangsmåde til anvendelse deraf - Google Patents

Filamentøs svampecelle med inaktiveret komponent fra den selektive autofagisignalvej og fremgangsmåde til anvendelse deraf Download PDF

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DK2981546T3
DK2981546T3 DK14715280.5T DK14715280T DK2981546T3 DK 2981546 T3 DK2981546 T3 DK 2981546T3 DK 14715280 T DK14715280 T DK 14715280T DK 2981546 T3 DK2981546 T3 DK 2981546T3
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filamentous fungal
fungal cell
fusarium
glu
cell
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DK14715280.5T
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Hiroaki Udagawa
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Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (15)

1. Filamentøs svampecelle som omfatter en komplet eller delvis inaktivering af den filamentøse svampecelles atg11-aktivitet, hvor cellen omfatter en ekspressionsvektor, som omfatter en sekvens af nukleotider, som koder for et heterologt enzym af interesse, og genetiske elementer, der er egnede til fremstilling af det heterologe enzym i den filamentøse svampecelle.
2. Filamentøs svampecelle ifølge krav 1, hvor det heterologe enzym af interesse er valgt fra gruppen bestående af en peptidase, (carboxypeptidase, aminopepetidase, protease), amylase (f.eks., alfa-amylase eller glucoamylase), carbohydrase, pullulanase, katalase, cellulase, chitinase, cutinase, cyclodextrin-glycosyltransferase, deoxyribonuclease, esterase, endo-glucosidase, alfa-galaktosidase, beta-galaktosidase, alfa-glucosidase, beta-glucosidase, invertase, laccase, lipase, phospholipase, mannosidase, mutanase, oxidase, pektinolytisk enzym, peroxidase, phytase, polyphenoloxidase, ribonuclease, transglutaminase og xylanase.
3. Filamentøs svampecelle ifølge krav 1, hvor det heterologe enzym af interesse er en glucoamylase.
4. Filamentøs svampecelle ifølge krav 1, hvor det heterologe enzym af interesse er en amylase.
5. Filamentøs svampecelle ifølge ethvert af de foregående krav, hvor den filamentøse svampecelle er valgt fra gruppen bestående af Acremonium, Aspergillus, Fusarium, Humicola, Mucor, Myceliophthora, Neurospora, Penicillium, Thielavia, Tolypocladium og Trichoderma.
6. Filamentøs svampecelle ifølge ethvert af de foregående krav, hvor den filamentøse svampecelle er valgt fra gruppen bestående af Aspergillus awamori, Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Fusarium bactridioides, Fusarium cerealis, Fusarium crookweiiense, Fusarium culmorum, Fusarium gram-inearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxyspo-rum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium sulphureum, Fusarium torulosum, Fusarium trichothecioi-des, Fusarium venenatum, Humicola insolens, Humicola lanuginosa, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Penicillium purpurogenum, Thielavia terrestris, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma ree-sei og Trichoderma viride-celle.
7. Filamentøs svampecelle ifølge ethvert af de foregående krav, hvor den filamentøse svampecelle er en Asperg/7/us-celle.
8. Filamentøs svampecelle ifølge ethvert af de foregående krav, hvor den filamentøse svampecelle er en Aspergillus niger-celle eller Aspergillus oryzae-celle.
9. Filamentøs svampecelle ifølge ethvert af de foregående krav, som omfatter en deletion eller disruption af de regulatoriske sekvenser, som ligger opstrøms eller nedstrøms for atg 11-genet.
10. Filamentøs svampecelle ifølge ethvert af kravene 1-9, som omfatter en deletion eller disruption af atg11 -genet.
11. Filamentøs svampecelle ifølge ethvert af de foregående krav, som omfatter en deletion af atg11-genet.
12. Filamentøs svampecelle ifølge ethvert af de foregående krav, hvor svampecellen yderligere omfatter en modificering for at forøge tilstedeværelsen af et had UPR moduleringsprotein i den filamentøse svampeværtscelle.
13. Filamentøs svampecelle ifølge ethvert af de foregående krav, hvor svampecellen yderligere omfatter en modificering for at forøge tilstedeværelsen af et ire1 UPR moduleringsprotein i den filamentøse svampeværtscelle.
14. Fremgangsmåde til fremstilling af et heterologt polypeptid af interesse omfattende dyrkning af en filamentøs svampecelle ifølge ethvert af de foregående krav under betingelser, der er fremmende for at fremkalde ekspression af et heterologt polypeptid af interesse.
15. Fremgangsmåde ifølge krav 14, som yderligere omfatter genindvinding af polypeptidet af interesse fra værtscelle-dyrkningsmediet.
DK14715280.5T 2013-04-03 2014-04-03 Filamentøs svampecelle med inaktiveret komponent fra den selektive autofagisignalvej og fremgangsmåde til anvendelse deraf DK2981546T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361807980P 2013-04-03 2013-04-03
PCT/EP2014/056689 WO2014161936A1 (en) 2013-04-03 2014-04-03 A filamentous fungal cell with inactivated component of the selective autophagy pathway and method of using same

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DK2981546T3 true DK2981546T3 (da) 2017-02-06

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US (1) US20160060641A1 (da)
EP (1) EP2981546B1 (da)
JP (2) JP2016518829A (da)
CN (1) CN105102471A (da)
DK (1) DK2981546T3 (da)
WO (1) WO2014161936A1 (da)

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JP2020536513A (ja) * 2017-10-02 2020-12-17 アストラゼネカ・アクチエボラーグＡｓｔｒａｚｅｎｅｃａ Ａｋｔｉｅｂｏｌａｇ タンパク質産生の増加のための細胞株及び方法
CN110358781B (zh) * 2019-07-31 2022-01-28 湖北大学 一种酸性哺乳动物几丁质酶编码基因和应用
CN116162555A (zh) * 2021-11-24 2023-05-26 中国科学院天津工业生物技术研究所 一种生产柠檬酸的工程菌及其构建方法和应用

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US6432914B1 (en) * 1996-09-13 2002-08-13 The Trustees Of Columbia University In The City Of New York Beclin and uses thereof
EP1266018B1 (en) * 2000-03-24 2008-05-07 Genencor International, Inc. Production of secreted proteins by recombinant eukaryotic cells
JP5881034B2 (ja) * 2011-03-01 2016-03-09 国立大学法人 東京大学 糸状菌変異株及びこれを用いたタンパク質の製造方法

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EP2981546A1 (en) 2016-02-10
CN105102471A (zh) 2015-11-25
US20160060641A1 (en) 2016-03-03
JP2019122386A (ja) 2019-07-25
WO2014161936A1 (en) 2014-10-09
EP2981546B1 (en) 2017-01-11
JP2016518829A (ja) 2016-06-30

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