DK2652138T3 - Promotorer til ekspression af gener i en svampecelle - Google Patents

Promotorer til ekspression af gener i en svampecelle Download PDF

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Publication number
DK2652138T3
DK2652138T3 DK11809006.7T DK11809006T DK2652138T3 DK 2652138 T3 DK2652138 T3 DK 2652138T3 DK 11809006 T DK11809006 T DK 11809006T DK 2652138 T3 DK2652138 T3 DK 2652138T3
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DK
Denmark
Prior art keywords
polypeptide
promoter
sequence
trichoderma reesei
host cell
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Application number
DK11809006.7T
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English (en)
Inventor
Sandra Merino
Debbie Yaver
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Novozymes Inc
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Publication of DK2652138T3 publication Critical patent/DK2652138T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (9)

1. Fremgangsmåde til fremstilling af et polypeptid omfattende: (a) dyrkning af en filamentøs svampeværtscelle i et medium, der er fremmende for fremstillingen af polypeptidet, hvor den filamentøse svampeværtscelle omfatter et polynukleotid, der koder for polypeptidet, der er operativt forbundet med en promotor, der er fremmed overfor polynukleotidet valgt fra gruppen bestående af (i) en promotor omfattende en nukleotidsekvens, der har mindst 60% sekvensidentitet med SEQ ID NO: 1, og (ii) en promotor omfattende SEQ ID NO: 1; og (b) isolering af polypeptidet fra dyrkningsmediet.
2. Fremgangsmåde ifølge krav 1, hvor promotoren omfatter en nukleotidsekvens med mindst 60%, mindst 65%, mindst 70%, mindst 75%, mindst 80%, mindst 81%, mindst 82%, mindst 83%, mindst 84%, mindst 85%, mindst 86%, mindst 87%, mindst 88%, mindst 89%, mindst 90%, mindst 91%, mindst 92%, mindst 93%, mindst 94%, mindst 95%, mindst 96%, mindst 97%, mindst 98%, mindst 99% eller 100% sekvensidentitet med SEQ ID NO: 1.
3. Fremgangsmåde ifølge krav 1, hvor promotoren omfatter eller består af polynukleotidsekvensen i SEQ ID NO: 1.
4. Fremgangsmåden ifølge ethvert af kravene 1-3, hvor polypeptidet er naturlig eller fremmed overfor den filamentøse svampeværtscelle.
5. En isoleret promotor valgt fra gruppen bestående af (i) en promotor omfattende en nukleotidsekvens, der har mindst 60% sekvensidentitet med SEQ ID NO: 1: og (ii) en promotor omfattende SEQ ID NO: 1.
6. Promotor ifølge krav 5, som omfatter en nukleotidsekvens med mindst 60%, mindst 65%, mindst 70%, mindst 75%, mindst 80%, mindst 85%, mindst 90%, mindst 91%, mindst 92%, mindst 93%, mindst 94%, mindst 95%, mindst 96%, mindst 97%, mindst 98%, mindst 99% eller 100% sekvensidentitet med SEQ ID NO: 1.
7. Promotor ifølge krav 5, som omfatter eller består af polynukleotidsekvensen i SEQ ID NO: 1.
8. Nukleotidsyrekonstrukt omfattende et polynukleotid, der koder for et polypeptid, der er operativt forbundet med promotoren ifølge ethvert af kravene 5-7, hvor polynukleotidet, der koder for polypeptidet, er fremmed overfor promotoren.
9. Rekombinant filamentøs svampeværtscelle omfattende nukleotidsyrekonstruktet ifølge krav 8.
DK11809006.7T 2010-12-16 2011-12-15 Promotorer til ekspression af gener i en svampecelle DK2652138T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42390910P 2010-12-16 2010-12-16
PCT/US2011/065287 WO2012083081A1 (en) 2010-12-16 2011-12-15 Promoters for expressing genes in a fungal cell

Publications (1)

Publication Number Publication Date
DK2652138T3 true DK2652138T3 (da) 2018-01-22

Family

ID=45496274

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11809006.7T DK2652138T3 (da) 2010-12-16 2011-12-15 Promotorer til ekspression af gener i en svampecelle

Country Status (6)

Country Link
US (2) US9284588B2 (da)
EP (1) EP2652138B1 (da)
CN (1) CN103261420B (da)
BR (1) BR112013014869A2 (da)
DK (1) DK2652138T3 (da)
WO (1) WO2012083081A1 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3089760B1 (en) * 2013-12-31 2020-01-15 ProteoNic Biotechnology IP B.V. Construct and sequence for enhanced gene expression
CN105779444B (zh) * 2014-12-16 2018-11-30 青岛蔚蓝生物集团有限公司 一种提高芽孢杆菌蛋白表达量的串联启动子
DK3268482T3 (da) 2015-03-09 2020-03-09 Novozymes As Fremgangsmåder til indføring af en flerhed af ekspressionskonstruktioner i en eukaryot celle
EP3296401B1 (en) * 2016-09-20 2020-02-19 Clariant International Ltd Method for selective carbon source-independent expression of protein-encoding sequences in a filamentous fungus cell

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
US5641670A (en) 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
BR9507817A (pt) 1994-06-03 1997-09-16 Novo Nordisk Biotech Inc Construção de dna enzima vetor recombinante célula hospedeira recombinante lacase de ascomicete ou deuteromicete processos para obter uma enzima de lacase para melhorar o rendimento de enzima recombinante para polimerizar um substrato de lignina ou lignossulfato em soluç o para despolimerizar in situ a pasta kraft para oxidar corantes ou precursores de corantes para pintar cabelo e para polimerizar ou oxidar um composto fenólico ou anilina composição de corante e recipiente contendo a mesma
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
DE69932345T2 (de) 1998-10-26 2007-07-19 Novozymes A/S Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
WO2000056900A2 (en) 1999-03-22 2000-09-28 Novo Nordisk Biotech, Inc. Promoter sequences derived from fusarium venenatum and uses thereof
EP1682656B1 (en) 2003-10-28 2013-09-18 Novozymes Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
WO2005056772A1 (en) 2003-12-09 2005-06-23 Novozymes Inc. Methods for eliminating or reducing the expression of genes in filamentous fungal strains
BRPI0714870A2 (pt) * 2006-07-21 2013-05-28 Novozymes Inc mÉtodo para produzir um polipeptÍdeo secretado tendo atividade biolàgica, proteÍna de fusço isolada, polinucleotÍdeo isolado, construÇço de proteÍna de fusço, vetor de expressço, cÉlula hospedeira féngica, mÉtodos para degradar ou conventer um material celulàsico e para produzir uma substÂncia
JP2012504390A (ja) 2008-09-30 2012-02-23 ノボザイムス，インコーポレイティド 糸状菌細胞におけるポジティブ及びネガティブ選択遺伝子の使用方法
CN101560513A (zh) * 2009-05-15 2009-10-21 邢苗 一种里氏木霉表达盒及其重组菌株和应用

Also Published As

Publication number Publication date
US9701970B2 (en) 2017-07-11
EP2652138B1 (en) 2017-11-01
CN103261420B (zh) 2016-01-20
CN103261420A (zh) 2013-08-21
WO2012083081A1 (en) 2012-06-21
BR112013014869A2 (pt) 2017-06-06
EP2652138A1 (en) 2013-10-23
US9284588B2 (en) 2016-03-15
US20130288298A1 (en) 2013-10-31
US20160152993A1 (en) 2016-06-02

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