DK2819783T3 - Prøvebehandlingsindretning med aftagelig plade - Google Patents
Prøvebehandlingsindretning med aftagelig plade Download PDFInfo
- Publication number
- DK2819783T3 DK2819783T3 DK13720547.2T DK13720547T DK2819783T3 DK 2819783 T3 DK2819783 T3 DK 2819783T3 DK 13720547 T DK13720547 T DK 13720547T DK 2819783 T3 DK2819783 T3 DK 2819783T3
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- DK
- Denmark
- Prior art keywords
- microfluidic
- chamber
- biological
- tissue
- processing device
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/71—Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
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- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Microbiology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Claims (13)
1. Behandlingsindretning til biologiske og kemiske prøver omfattende - en mikrofluidindretning, -en anordning af mikrofluidadgangshuller i mikrofluidindretningen til henholdsvis at indsprøjte fluid i og opsamle fluid fra et fladt mikrofluidkammer, hvor anordningen af mikrofluidadgangshuller er udformet til advektiv transport af flui-diske substanser og reagenser i mikrofluidkammeret på en ensartet måde, - indløbsåbninger og mikrofluidkanaler, der er udformet uden på kammeret, hvor mikrofluidkanalerne er forbundet med mikrofluidadgangshullerne og kendetegnet ved - en individuelt tilpasset tætnende O-ring, der er positioneret på en side af mikrofluidindretningen, hvor behandlingsindretningen til biologiske og kemiske prøver er udformet således, at en aftagelig plade, der indeholder prøver, såsom immobiliserede enheder, biologiske prøver eller molekyler kan bringes i kontakt med mikrofluidindretningens tætningsring til dannelse af det mikroflui-diske og højtrykbestandige og flade mikrofluidkammer med stor flade og mindst en væg, der udgøres af den aftagelige plade.
2. Behandlingsindretning til biologiske og kemiske prøver ifølge krav 1, der omfatter trykdrevne flowgenererende midler, der bevirker en trykforskel mellem indløb og udløb til frembringelse af et fluidflow inden i mikrofluidkammeret.
3. Behandlingsindretning til biologiske og kemiske prøver ifølge krav 1, omfattende elektrokinetisk flow-genererende midler til tilvejebringelse af et fluidflow inden i mikrofluidkammeret ved at påføre en elektrisk potentialdifference mellem to vilkårlige eller flere punkter af mikrofluidkammeret, mikrofluidkanalerne og mikrofluidadgangshullerne.
4. Behandlingsindretning til biologiske og kemiske prøver ifølge krav 1, omfattende termisk induceret flow-genererende midler til tilvejebringelse af et fluidflow inden i mikrofluidkammeret ved at inducere fluidisk flow ved at generere en temperaturdifference mellem to vilkårlige eller flere punkter af mikrofluidkammeret, mikrofluidkanalerne og mikrofluidadgangshullerne.
5. Behandlingsindretning til biologiske og kemiske prøver ifølge et af de foregående krav, der omfatter et varmeelement og en temperatursensor til at indstille og styre temperaturen på fluiden, der er indesluttet i mikrofluidkammeret, mikrofluidkanalerne og mikrofluidadgangshullerne.
6. Behandlingsindretning til biologiske og kemiske prøver ifølge et af de foregående krav, der inkorporer lysdetektorer og -kilder eller et array af lysdetektorer og -kilder, der er fremstillet med siliciummikroelektronikteknologi til digital billeddannelse af enheder, der er immobiliseret på en glasplade til lysfelt- eller fluorescensdetektering.
7. Behandlingsindretning til biologiske og kemiske prøver ifølge et af de foregående krav, der omfatter mikrolinse-arrays, polariserings- og/eller fluorescenslysfiltre, der er anbragt foran lysdetektorer og -kilder eller et array af disse lysdetektorer og -kilder.
8. Behandlingsindretning til biologiske og kemiske prøver ifølge et af de foregående krav, hvor kammerhøjden er mindre end 100 pm.
9. Behandlingsindretning til biologiske og kemiske prøver ifølge et af de foregående krav, hvor mikrofluidadgangshullerne til indstrømning og udstrømning er anbragt langs kanter af mikrofluidkammeret.
10. Behandlingsindretning til biologiske og kemiske prøver ifølge et af de foregående krav, hvor mikrofluidadgangshullerne omfatter huller til indstrømning, der er anbragt langs en kant af mikrofluidkammeret, og huller til udstrømning, der er anbragt langs en modsat kant af mikrofluidkammeret.
11. Behandlingsindretning til biologiske og kemiske prøver ifølge et af de foregående krav, hvor mikrofluidadgangshullerne er vertikale og strækker sig mellem en topside og en bundside af mikrofluidindretningen, hvor bundsiden vender mod mikrofluidkammeret, og mikrofluidkanalerne danner en fordelt netværkskanal på topsiden.
12. Fremgangsmåde til behandling af biologiske og kemiske prøver, der udføres ved anvendelse af behandlingsindretningen til biologiske og kemiske prøver ifølge det foregående krav, omfattende trinnene: - at opnå pladerne med enheder på en eller flere sider manuelt eller automatisk; - at etablere det dannede kammer ved at inkorporere pladerne i mikrofluidind-retningen manuelt eller automatisk; - at udsætte enhederne på pladerne sekventielt for passende fluidiske eller reaktive substanser i foruddefinerede tidsrum og ved foruddefinerede temperaturer; - at udsætte enhederne på pladerne sekventielt for passende fluidiske eller reaktive substanser i foruddefinerede tidsrum og ved foruddefinerede temperaturer til indikation på detektering.
13. Anvendelse af en indretning ifølge et af kravene 1 til 11 til at udsætte enheder på pladens overflade for en passende fluidisk eller reaktiv substans eller en sekvens af passende fluidiske reaktive substanser i foruddefinerede tidsrum, hvor anvendelsen omfatter mindst en af følgende: i- en histokemisk proces ii. en cytokemisk proces iii. en immunohistokemisk proces iv. en immunocytokemisk proces v. en immunohistofluorescensproces vi. en immunocytofluorescensproces vii. en in situ-hybridiseringsproces viii. en in situ-fluorescenshybridiseringsproces ix. en antigenhentningsproces X. en epitophentningsproces xi. en varmeinduceret antigenhentningsproces xii. en varmeinduceret epitophentningsproces xiii. en paraffinopløsnings- eller ætsningsproces xiv. en farvningproces
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| KR102125410B1 (ko) * | 2015-02-26 | 2020-06-22 | 프라운호퍼 게젤샤프트 쭈르 푀르데룽 데어 안겐반텐 포르슝 에. 베. | 타깃 시간 도메인 포락선을 사용하여 처리된 오디오 신호를 얻도록 오디오 신호를 처리하기 위한 장치 및 방법 |
| WO2016168429A1 (en) | 2015-04-14 | 2016-10-20 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University | Method and apparatus for extracting and collecting single cells from formalin-fixed paraffin embedded tissues |
| TWI712686B (zh) * | 2015-04-22 | 2020-12-11 | 美商柏克萊燈光有限公司 | 用於微流體裝置之培養站 |
| AU2017217609B2 (en) * | 2016-02-08 | 2023-03-09 | Lunaphore Technologies Sa | Methods of sample cycle multiplexing and in situ imaging |
| EP3222351A1 (en) | 2016-03-23 | 2017-09-27 | Ecole Polytechnique Federale de Lausanne (EPFL) | Microfluidic network device |
| EP3427829A1 (en) * | 2017-07-12 | 2019-01-16 | Lunaphore Technologies SA | Methods of in situ antigen retrieval of a biological sample & imaging thereof |
| WO2019046613A1 (en) * | 2017-08-30 | 2019-03-07 | Microbedx, Inc. | DEVICE FOR OPTIMIZING THE GROWTH OF MICROORGANISMS IN LIQUID CULTURE |
| US10706535B2 (en) * | 2017-09-08 | 2020-07-07 | International Business Machines Corporation | Tissue staining quality determination |
| EP3459632A1 (en) | 2017-09-26 | 2019-03-27 | Lunaphore Technologies SA | Microfluidic cartrige with built-in sampling device |
| CN111215159A (zh) * | 2018-11-26 | 2020-06-02 | 南京怡天生物科技有限公司 | 微流控芯片及基于该芯片融合样本的方法 |
| EP3798640A1 (en) * | 2019-09-27 | 2021-03-31 | Lunaphore Technologies SA | Biological sample processing system and microfluidic cartridge therefor |
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| US5188963A (en) * | 1989-11-17 | 1993-02-23 | Gene Tec Corporation | Device for processing biological specimens for analysis of nucleic acids |
| CA2074214C (en) * | 1991-07-23 | 2000-02-22 | Will Bloch | Improvements in the in situ pcr |
| EP0849992B1 (en) * | 1995-06-07 | 2004-08-25 | WHATMAN plc | Dry solid medium for storage and analysis of genetic material |
| AU2001297830A1 (en) * | 2000-10-25 | 2002-12-09 | Exiqon A/S | Closed substrate platforms suitable for analysis of biomolecules |
| EP1372848A4 (en) * | 2001-03-09 | 2006-08-09 | Biomicro Systems Inc | METHOD AND SYSTEM FOR MICROFLUIDIC INTERFERENCE WITH NETWORKS |
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| ATE288316T1 (de) | 2001-05-25 | 2005-02-15 | Tecan Trading Ag | System mit verfahrenseinheit zum hybridisieren von nukleinsäureproben, proteinen und gewebeschnitten |
| WO2003015922A1 (en) | 2001-08-20 | 2003-02-27 | Biomicro Systems, Inc. | Laminated microarray interface device |
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| DE10316723A1 (de) * | 2003-04-09 | 2004-11-18 | Siemens Ag | Probenplatte zum Einbau in eine Gehäusestruktur |
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| WO2011026128A2 (en) | 2009-08-31 | 2011-03-03 | Life Technologies Corporation | Flowcells and methods of filling and using same |
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| JP6318094B2 (ja) | 2018-05-09 |
| US10436683B2 (en) | 2019-10-08 |
| US20150005190A1 (en) | 2015-01-01 |
| CN104284725A (zh) | 2015-01-14 |
| SG11201405196VA (en) | 2014-09-26 |
| KR20140129181A (ko) | 2014-11-06 |
| ES2704989T3 (es) | 2019-03-21 |
| HUE042751T2 (hu) | 2019-07-29 |
| AU2013227390A1 (en) | 2014-09-11 |
| KR102079307B1 (ko) | 2020-02-19 |
| TR201900296T4 (tr) | 2019-02-21 |
| EP2819783A1 (en) | 2015-01-07 |
| WO2013128322A1 (en) | 2013-09-06 |
| JP2015517088A (ja) | 2015-06-18 |
| CN104284725B (zh) | 2017-04-26 |
| CA2902127A1 (en) | 2013-09-06 |
| PL2819783T3 (pl) | 2019-07-31 |
| AU2013227390B2 (en) | 2016-04-14 |
| EP2819783B1 (en) | 2018-10-10 |
| CA2902127C (en) | 2021-08-10 |
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