DK2773958T3 - Fremgangsmåde til identifikation af en analyt i en biologisk prøve - Google Patents
Fremgangsmåde til identifikation af en analyt i en biologisk prøve Download PDFInfo
- Publication number
- DK2773958T3 DK2773958T3 DK12790972.9T DK12790972T DK2773958T3 DK 2773958 T3 DK2773958 T3 DK 2773958T3 DK 12790972 T DK12790972 T DK 12790972T DK 2773958 T3 DK2773958 T3 DK 2773958T3
- Authority
- DK
- Denmark
- Prior art keywords
- aptamer
- analyte
- sers
- sam
- dna
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56905—Protozoa
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/5695—Mycobacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3517—Marker; Tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2610/00—Assays involving self-assembled monolayers [SAMs]
Claims (8)
1. Fremgangsmåde til identifikation af et analytmolekyle i en biologisk prøve, hvilken fremgangsmåde omfatter trinnene: opfangning af analytmolekylet på en metaloverflade, hvortil der er bundet et selvmonteret monolag (SAM) af amfifile molekyler, der er kovalent coatet med et lag af oligoethylenglycolmolekyler, idet analytmolekylet er opfanget ved hjælp af en analytmolekylspecifik aptamer, der er bundet direkte til de amfifile molekyler af SAM eller bundet direkte til metaloverfladen og omgivet af SAM, hvilket resulterer i et aptamer-analytmolekyle-kompleks på metaloverfladen, for hvilket et reproducerbart og unikt SERS-spektrum kan måles, måling af SERS-signalintensiteten og det specifikke SERS-spektrum af det resulterende aptamer-analytmolekyle-kompleks og identifikation af det opfangede analytmolekyle ved at sammenligne det målte SERS-spektrum af aptamer-analytmolekyle-komplekset med en database med reference-SERS-spektre for at verificere identiteten af det opfangede analytmolekyle.
2. Fremgangsmåde ifølge krav 1, som yderligere omfatter et trin med at sammenligne den målte SERS-signalintensitet med en standardkurve for at kvantificere hyppigheden af den opfangede analyt.
3. Fremgangsmåde ifølge et hvilket som helst af kravene 1 eller 2, hvor verifikation af identiteten af det opfangede analytmolekyle skelner mellem sande og falske positive resultater i kvantitative analytassays på komplekse biologiske prøver.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor aptameren er en DNA-aptamer.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, hvor aptameren er bundet direkte til de amfifile molekyler af SAM.
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, hvor aptameren er bundet direkte til metaloverfladen og omgivet af et selvmonteret monolag (SAM).
7. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 6, hvor oligoethylengly-colmolekylerne har eksponerede terminaler og er derivatiseret på terminalerne af aptameren.
8. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 7 til diagnosticering eller kvantificering af en infektion, sygdom eller medicinsk tilstand i et individ, hvorfra prøven er blevet taget, eller til overvågning af et individ under anæstesi.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ZA201108016 | 2011-11-02 | ||
PCT/IB2012/056108 WO2013065016A1 (en) | 2011-11-02 | 2012-11-02 | A method of detecting and/or quantifying an analyte in a biological sample |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2773958T3 true DK2773958T3 (da) | 2018-03-12 |
Family
ID=47222168
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK12790972.9T DK2773958T3 (da) | 2011-11-02 | 2012-11-02 | Fremgangsmåde til identifikation af en analyt i en biologisk prøve |
Country Status (8)
Country | Link |
---|---|
US (1) | US9518986B2 (da) |
EP (1) | EP2773958B1 (da) |
CN (1) | CN104380105B (da) |
BR (1) | BR112014010696A2 (da) |
DK (1) | DK2773958T3 (da) |
IN (1) | IN2014KN01168A (da) |
WO (1) | WO2013065016A1 (da) |
ZA (1) | ZA201403386B (da) |
Families Citing this family (19)
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WO2015143247A1 (en) * | 2014-03-19 | 2015-09-24 | The Regents Of The University Of Colorado, A Body Corporate | Binding detection using liquid crystal |
EP3259286A4 (en) * | 2015-02-19 | 2018-07-11 | Ionica Sciences | Reagents and methods for detecting infectious diseases |
CN105510411B (zh) * | 2015-09-14 | 2018-05-08 | 陕西师范大学 | 基于细胞与微电极的相互作用实现单一癌细胞的检测方法 |
DE102015220414A1 (de) * | 2015-10-20 | 2017-04-20 | Robert Bosch Gmbh | Propofolsensor zur Messung einer Narkosetiefe |
CN105277527A (zh) * | 2015-10-29 | 2016-01-27 | 江南大学 | 一种基于拉曼信标分子编码银@金核壳纳米粒子的真菌毒素双重检测方法 |
KR101816521B1 (ko) * | 2016-01-28 | 2018-01-09 | 광주과학기술원 | 삽입제를 이용한 국소표면플라즈몬공명 기반 고감도 압타머 센서 |
US10103357B2 (en) | 2016-02-17 | 2018-10-16 | The Curators Of The University Of Missouri | Fabrication of multilayer nanograting structures |
US11144576B2 (en) | 2016-10-28 | 2021-10-12 | Hewlett-Packard Development Company, L.P. | Target class feature model |
KR101923196B1 (ko) * | 2017-09-25 | 2018-11-28 | 주식회사 엠디엡투스 | Cfp10에 특이적으로 결합하는 dna 압타머 및 이의 용도 |
US20210190774A1 (en) * | 2017-12-18 | 2021-06-24 | The University Of Memphis Research Foundation | Compositions and methods for the detection and molecular profiling of membrane bound vesicles |
TWI686599B (zh) * | 2018-05-24 | 2020-03-01 | 國立清華大學 | 塵蟎抗原檢測系統及方法 |
WO2020100159A1 (en) * | 2018-11-13 | 2020-05-22 | Translational Health Science And Technology Institute | A novel aptamer and an electrochemical biosensor for the rapid detection and diagnosis of tuberculous meningitis |
TWI693403B (zh) * | 2019-01-17 | 2020-05-11 | 逢甲大學 | 免疫檢測方法 |
WO2020209796A2 (en) * | 2019-04-09 | 2020-10-15 | Agency For Science, Technology And Research | Label-free detection of mycobacteria using surface enhanced raman spectroscopy |
CN110907643A (zh) * | 2019-12-02 | 2020-03-24 | 中国科学院重庆绿色智能技术研究院 | 一种大肠杆菌检测芯片的制备方法及检测芯片 |
TWI759776B (zh) * | 2020-06-22 | 2022-04-01 | 淡江大學 | 檢測基板、拉曼光譜檢測系統及拉曼光譜檢測方法 |
JP2023546790A (ja) * | 2020-09-24 | 2023-11-08 | イノテック プリシジョン メディスン インコーポレイテッド | 病原体を検出するためのシステム、装置、および方法 |
CN112240881A (zh) * | 2020-09-30 | 2021-01-19 | 合肥国研汉因检测科技有限公司 | 使用拉曼光谱法快速检测痕量芬太尼的方法 |
CN112986212B (zh) * | 2021-03-10 | 2023-01-17 | 中国人民解放军海军军医大学 | 一种g-四链体核酸适配体与小分子相互作用的研究方法 |
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-
2012
- 2012-11-02 WO PCT/IB2012/056108 patent/WO2013065016A1/en active Application Filing
- 2012-11-02 US US14/355,765 patent/US9518986B2/en active Active
- 2012-11-02 CN CN201280065543.0A patent/CN104380105B/zh not_active Expired - Fee Related
- 2012-11-02 DK DK12790972.9T patent/DK2773958T3/da active
- 2012-11-02 BR BR112014010696A patent/BR112014010696A2/pt not_active Application Discontinuation
- 2012-11-02 EP EP12790972.9A patent/EP2773958B1/en not_active Not-in-force
- 2012-11-02 IN IN1168KON2014 patent/IN2014KN01168A/en unknown
-
2014
- 2014-05-12 ZA ZA2014/03386A patent/ZA201403386B/en unknown
Also Published As
Publication number | Publication date |
---|---|
BR112014010696A2 (pt) | 2017-04-25 |
WO2013065016A1 (en) | 2013-05-10 |
IN2014KN01168A (da) | 2015-10-16 |
US9518986B2 (en) | 2016-12-13 |
EP2773958B1 (en) | 2017-11-29 |
CN104380105B (zh) | 2017-04-12 |
CN104380105A (zh) | 2015-02-25 |
EP2773958A1 (en) | 2014-09-10 |
ZA201403386B (en) | 2016-02-24 |
US20140302492A1 (en) | 2014-10-09 |
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