DK2681231T3 - PROCESS FOR THE ISOLATION OF osteopontin USING CONCENTRATED RAW MATERIALS - Google Patents
PROCESS FOR THE ISOLATION OF osteopontin USING CONCENTRATED RAW MATERIALS Download PDFInfo
- Publication number
- DK2681231T3 DK2681231T3 DK12707103.3T DK12707103T DK2681231T3 DK 2681231 T3 DK2681231 T3 DK 2681231T3 DK 12707103 T DK12707103 T DK 12707103T DK 2681231 T3 DK2681231 T3 DK 2681231T3
- Authority
- DK
- Denmark
- Prior art keywords
- milk
- raw material
- protein
- derived
- range
- Prior art date
Links
- 102000004264 Osteopontin Human genes 0.000 title claims description 133
- 108010081689 Osteopontin Proteins 0.000 title claims description 133
- 238000000034 method Methods 0.000 title claims description 79
- 239000002994 raw material Substances 0.000 title claims description 42
- 230000008569 process Effects 0.000 title claims description 17
- 238000002955 isolation Methods 0.000 title description 6
- 235000013336 milk Nutrition 0.000 claims description 268
- 239000008267 milk Substances 0.000 claims description 268
- 210000004080 milk Anatomy 0.000 claims description 268
- 235000018102 proteins Nutrition 0.000 claims description 144
- 102000004169 proteins and genes Human genes 0.000 claims description 144
- 108090000623 proteins and genes Proteins 0.000 claims description 144
- 108010046377 Whey Proteins Proteins 0.000 claims description 75
- 102000007544 Whey Proteins Human genes 0.000 claims description 73
- 239000005862 Whey Substances 0.000 claims description 65
- 238000005349 anion exchange Methods 0.000 claims description 63
- 235000009508 confectionery Nutrition 0.000 claims description 32
- 210000002966 serum Anatomy 0.000 claims description 27
- 239000000203 mixture Substances 0.000 claims description 25
- 238000005571 anion exchange chromatography Methods 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- 102000004407 Lactalbumin Human genes 0.000 claims description 9
- 108090000942 Lactalbumin Proteins 0.000 claims description 9
- 235000021241 α-lactalbumin Nutrition 0.000 claims description 9
- 102000008192 Lactoglobulins Human genes 0.000 claims description 8
- 108010060630 Lactoglobulins Proteins 0.000 claims description 8
- 238000011026 diafiltration Methods 0.000 claims description 4
- 238000001694 spray drying Methods 0.000 claims description 4
- 150000001768 cations Chemical class 0.000 claims description 3
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- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 28
- 235000021240 caseins Nutrition 0.000 description 28
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- 239000012141 concentrate Substances 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 12
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- 238000007696 Kjeldahl method Methods 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
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- 108010001441 Phosphopeptides Proteins 0.000 description 2
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- 239000007983 Tris buffer Substances 0.000 description 2
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- 229910052791 calcium Inorganic materials 0.000 description 2
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- 235000021243 milk fat Nutrition 0.000 description 2
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- 238000010979 pH adjustment Methods 0.000 description 2
- 230000020477 pH reduction Effects 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 125000001453 quaternary ammonium group Chemical group 0.000 description 2
- 235000020185 raw untreated milk Nutrition 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 229940108461 rennet Drugs 0.000 description 2
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 235000021246 κ-casein Nutrition 0.000 description 2
- CHRJZRDFSQHIFI-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC1=CC=CC=C1C=C CHRJZRDFSQHIFI-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- DFYULHRIYLAUJM-UHFFFAOYSA-N 3,4-diiodobenzoic acid Chemical compound OC(=O)C1=CC=C(I)C(I)=C1 DFYULHRIYLAUJM-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 235000002198 Annona diversifolia Nutrition 0.000 description 1
- 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
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- 102000003886 Glycoproteins Human genes 0.000 description 1
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- 241000282842 Lama glama Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 1
- 102000014171 Milk Proteins Human genes 0.000 description 1
- 108010011756 Milk Proteins Proteins 0.000 description 1
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- 206010029148 Nephrolithiasis Diseases 0.000 description 1
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- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Peptides Or Proteins (AREA)
- Dairy Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161448775P | 2011-03-03 | 2011-03-03 | |
| EP11156826 | 2011-03-03 | ||
| PCT/EP2012/053749 WO2012117120A1 (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using concentrated feeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2681231T3 true DK2681231T3 (en) | 2016-03-21 |
Family
ID=44202290
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12707103.3T DK2681231T3 (en) | 2011-03-03 | 2012-03-05 | PROCESS FOR THE ISOLATION OF osteopontin USING CONCENTRATED RAW MATERIALS |
| DK12706623.1T DK2681230T3 (en) | 2011-03-03 | 2012-03-05 | A method for isolating osteopontin BY APPLICATION OF RAW MATERIALS CONTAINING CASEINOMAKROPEPTID (CMP) |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12706623.1T DK2681230T3 (en) | 2011-03-03 | 2012-03-05 | A method for isolating osteopontin BY APPLICATION OF RAW MATERIALS CONTAINING CASEINOMAKROPEPTID (CMP) |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US9181325B2 (https=) |
| EP (2) | EP2681230B1 (https=) |
| JP (2) | JP6012053B2 (https=) |
| KR (2) | KR101940829B1 (https=) |
| CN (3) | CN105085649B (https=) |
| AR (2) | AR085620A1 (https=) |
| AU (2) | AU2012222312B2 (https=) |
| BR (2) | BR112013022251B1 (https=) |
| CA (2) | CA2828669C (https=) |
| DK (2) | DK2681231T3 (https=) |
| EA (2) | EA023367B1 (https=) |
| ES (2) | ES2565380T3 (https=) |
| MX (2) | MX2013010017A (https=) |
| PL (2) | PL2681231T3 (https=) |
| WO (2) | WO2012117120A1 (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9337668B2 (en) * | 2011-04-28 | 2016-05-10 | Zoll Circulation, Inc. | Viral distribution of battery management parameters |
| EA201491691A1 (ru) | 2012-03-28 | 2015-04-30 | Арла Фудз Амба | Агрегаты наночастиц, содержащие остеопонтин и кальций- и/или стронцийсодержащие частицы |
| ES2656474T3 (es) * | 2013-07-05 | 2018-02-27 | Arla Foods Amba | Osteopontina láctea de origen mamífero para potenciar la capacidad de respuesta inmune |
| US9661868B2 (en) * | 2013-07-16 | 2017-05-30 | Mead Johnson Nutrition Company | Expanded bed adsorption methods for isolation of basic milk proteins including lactoferrin |
| KR200482960Y1 (ko) | 2016-04-07 | 2017-03-21 | 오숙희 | 고주파 얼굴 축소 시술기 |
| IL310614A (en) * | 2016-07-06 | 2024-04-01 | Building Block Nutritionals Llc | Nutritional formulation |
| EP3538053B1 (en) | 2016-11-08 | 2026-04-15 | Dentherapy Ltd | Compositions comprising fluoride and calcium and method for preparing them |
| EP3727032A1 (en) * | 2017-12-22 | 2020-10-28 | Société des Produits Nestlé S.A. | Extracts and their use |
| CN109613140B (zh) * | 2019-01-07 | 2021-10-08 | 雅士利国际集团有限公司 | 食品中酪蛋白磷酸肽的检测方法 |
| IL299098A (en) | 2020-06-15 | 2023-02-01 | Building Block Nutritionals Llc | Nutritional formula |
| GB202010987D0 (en) | 2020-07-16 | 2020-09-02 | Dentherapy Ltd | Oral care compositions and methods |
| EP4326076A1 (en) * | 2021-04-19 | 2024-02-28 | Société des Produits Nestlé S.A. | Use of milk protein fractions as a source of osteopontin |
| CN113567573B (zh) * | 2021-07-09 | 2023-06-02 | 黑龙江飞鹤乳业有限公司 | 骨桥蛋白的分离方法和分析方法 |
| GB202110114D0 (en) | 2021-07-14 | 2021-08-25 | Dentherapy Ltd | Oral care compositions for use in treaatment |
| EP4587455A1 (en) | 2022-09-16 | 2025-07-23 | Univerza V Ljubljani | Isolation of osteopontin and glycomacropeptide from whey |
| WO2025008636A1 (en) | 2023-07-06 | 2025-01-09 | Dentherapy Ltd | Oral care compositions for use in the treatment of dental health conditions |
| CN117837766A (zh) * | 2023-12-27 | 2024-04-09 | 美维仕(北京)健康管理有限公司 | 营养组合物及其应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998014071A1 (en) * | 1996-10-01 | 1998-04-09 | John Stephen Ayers | Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5 %w/w |
| JP2001527030A (ja) | 1997-12-31 | 2001-12-25 | デピュイ・オーソピーディックス・インコーポレイテッド | 骨修復効果を向上するためのオステオポンチンを基剤とする組成物 |
| PT1308456E (pt) * | 1998-05-06 | 2007-12-03 | Genentech Inc | Purificação de anticorpos por cromatografia de permuta iónica |
| DE10085286T1 (de) * | 1999-12-08 | 2002-11-21 | Univ Massey | Verfahren zur Trennung von Molkenproteinen, das einen neuartigen Anionenaustauscher verwendet |
| US7259243B2 (en) * | 2000-01-07 | 2007-08-21 | Arla Foods Amba | Process for isolation of osteopontin from milk |
| NZ507335A (en) * | 2000-10-05 | 2004-10-29 | New Zealand Dairy Board | Bone health compositions derived from milk comprising an acidic protein fraction but that does not contain CGMP |
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