DK2622071T3 - Polypeptider med endopeptidaseaktivitet og polynukleotider, der koder for dem - Google Patents

Polypeptider med endopeptidaseaktivitet og polynukleotider, der koder for dem Download PDF

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Publication number
DK2622071T3
DK2622071T3 DK11763735.5T DK11763735T DK2622071T3 DK 2622071 T3 DK2622071 T3 DK 2622071T3 DK 11763735 T DK11763735 T DK 11763735T DK 2622071 T3 DK2622071 T3 DK 2622071T3
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DK
Denmark
Prior art keywords
polypeptide
ala
gly
thr
endopeptidase
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DK11763735.5T
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English (en)
Inventor
Peter R Oestergaard
Carsten P SÖNKSEN
Tine Hoff
Gitte B Lynglev
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Novozymes As
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Publication of DK2622071T3 publication Critical patent/DK2622071T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/347—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins from microorganisms or unicellular algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Dairy Products (AREA)
  • Peptides Or Proteins (AREA)

Claims (10)

1. Isoleret polypeptid med endopeptidaseaktivitet, der er udvalgt fra gruppen, som består af: (a) et polypeptid, som udviser mindst 70% sekvensidentitet med det modne polypeptid ifølge SEQ ID NO: 2; (b) et polypeptid, der kodes for af et polynukleotid, som udviser mindst 70% sekvensidentitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 1; og (c) et fragment af et polypeptid ifølge (a) eller (b), der har endopeptidaseaktivitet.
2. Polypeptid ifølge krav 1, som udviser mindst 75%, mindst 80%, mindst 85%, mindst 90%, mindst 95%, mindst 96%, mindst 97%, mindst 98%, mindst 99% eller 100% sekvensidentitet med det modne polypeptid ifølge SEQ ID NO: 2.
3. Polypeptid ifølge et hvilket som helst af kravene 1-2, som kodes for af et polynukleotid, som udviser mindst 75%, mindst 80%, mindst 85%, mindst 90%, mindst 95%, mindst 96%, mindst 97%, mindst 98%, mindst 99% eller 100% sekvensidentitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 1.
4. Polypeptid ifølge et hvilket som helst af kravene 1-3, der omfatter eller består af SEQ ID NO: 2.
5. Polypeptid ifølge et hvilket som helst af kravene 1-4, der kodes for af polynukleotidet, som er indeholdt i plasmid ExpVec8, der er indeholdt i E. coli DSM 23706.
6. Sammensætning, som omfatter polypeptidet ifølge et hvilket som helst af kravene 1-5.
7. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-5, hvilken fremgangsmåde omfatter: (a) dyrkning af en rekombinant værtscelle, som omfatter et polynukleotid, der koder for polypeptidet ifølge et hvilket som helst af kravene 1-5, og som er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
8. Anvendelse af polypeptidet ifølge et hvilket som helst af kravene 1-5 til fremstilling af et proteinhydrolysat.
9. Fremgangsmåde til fremstilling af et proteinhydrolysat, hvilken fremgangsmåde omfatter: (a) tilvejebringelse af en opløsning, som omfatter protein, der skal hydrolyseres; (b) tilsætning af polypeptidet ifølge et hvilket som helst af kravene 1-5 til opløsningen og (c) opnåelse af proteinhydrolysatet.
10. Fremgangsmåde ifølge krav 9, hvor proteinet, der skal hydrolyseres, er fødevareprotein.
DK11763735.5T 2010-10-01 2011-09-30 Polypeptider med endopeptidaseaktivitet og polynukleotider, der koder for dem DK2622071T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10185793 2010-10-01
PCT/EP2011/067167 WO2012042037A1 (en) 2010-10-01 2011-09-30 Polypeptides having endopeptidase activity and polynucleotides encoding same

Publications (1)

Publication Number Publication Date
DK2622071T3 true DK2622071T3 (da) 2016-03-07

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DK11763735.5T DK2622071T3 (da) 2010-10-01 2011-09-30 Polypeptider med endopeptidaseaktivitet og polynukleotider, der koder for dem

Country Status (10)

Country Link
US (1) US9458446B2 (da)
EP (1) EP2622071B1 (da)
JP (1) JP5944908B2 (da)
CN (1) CN103534349B (da)
BR (1) BR112013004743A2 (da)
CA (1) CA2809916A1 (da)
DK (1) DK2622071T3 (da)
MX (1) MX343295B (da)
RU (1) RU2583293C2 (da)
WO (1) WO2012042037A1 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2904101T3 (da) 2012-10-03 2019-08-12 Agrivida Inc Intein-modificerede proteaser, fremstilling deraf og industrielle anvendelser
CN113164563A (zh) * 2018-07-23 2021-07-23 因柯利尔疗法公司 神经性病症的治疗方法
CN110283880A (zh) * 2019-08-08 2019-09-27 天津科技大学 一种测定蛋白酶活力的新方法
WO2023190529A1 (ja) * 2022-03-30 2023-10-05 森永乳業株式会社 乳タンパク質分解物の製造方法

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Also Published As

Publication number Publication date
CN103534349B (zh) 2017-07-21
JP2013544499A (ja) 2013-12-19
EP2622071B1 (en) 2015-12-09
WO2012042037A1 (en) 2012-04-05
US20130273203A1 (en) 2013-10-17
JP5944908B2 (ja) 2016-07-05
MX2013003323A (es) 2013-06-24
US9458446B2 (en) 2016-10-04
EP2622071A1 (en) 2013-08-07
MX343295B (es) 2016-11-01
CA2809916A1 (en) 2012-04-05
CN103534349A (zh) 2014-01-22
RU2583293C2 (ru) 2016-05-10
RU2013120189A (ru) 2014-11-20
BR112013004743A2 (pt) 2016-06-07

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