DK251682A - PROCEDURE FOR THE PREPARATION OF A MICRO-ORGANISM FORMING ALFA-GALACTOSIDASE BUT NOT INVERTASE - Google Patents
PROCEDURE FOR THE PREPARATION OF A MICRO-ORGANISM FORMING ALFA-GALACTOSIDASE BUT NOT INVERTASE Download PDFInfo
- Publication number
- DK251682A DK251682A DK251682A DK251682A DK251682A DK 251682 A DK251682 A DK 251682A DK 251682 A DK251682 A DK 251682A DK 251682 A DK251682 A DK 251682A DK 251682 A DK251682 A DK 251682A
- Authority
- DK
- Denmark
- Prior art keywords
- dna
- cells
- isolates
- alpha
- transformable
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
1. Claims (for the Contracting States BE, CH, DE, FR, GB, IT, LI, LU, NL, SE) Process for the production of a micro-organism which constitutively forms an alpha-galactosidase, which does not need cofactors, but not invertase, characterised in that one fully splits not only a DNA containing an alpha-galactosidase gene but also a vector suitable for the transformable cells to be used, which contains antibiotic-resistant genes, with restriction endonuclease Sal I, obtains from the fragments of the DNA containing the alpha-galactosidase gene the fragment of about four megadalton relative molecular weight, mixes with the solution of the vector also split with Sal I and recombines in the presence of DNA ligase with formation of a recombinant DNA, incubates the recombinant DNA obtained with transformable E. coli cells with transformation of the recombinant DNA into the cells, cultures the transformed cells on a nutrient medium containing raffinose as sole C-source, isolates and lyses the antibiotic-resistant colonies formed, isolates from the lysate the plasmid DNA, splits this with restriction endonuclease Hind III or Eco R I, dilutes the solution obtained and treats with DNA ligase, again introduces the renatured plasmids obtained into transformable cells which constitutively do not form invertase, again cultures the transformed cells on a nutrient medium containing raffinose as C-source, as well as antibiotic, and isolates the colonies formed which do not utilise raffinose. 1. Claims (for the Contracting State AT) Process for the production of a micro-organism which constitutively forms an alpha-galactosidase, which does not need cofactors, but not invertase, characterised in that one fully splits not only a DNA containing an alpha-galactosidase gene but also a vector suitable for the transformable cells to be used, which contains antibiotic-resistant genes, with restriction endonuclease Sal I, obtains from the fragments of the DNA containing the alpha-galactosidase gene the fragment of about four megadalton relative molecular weight, mixes with the solution of the vector also split with Sal I and recombines in the presence of DNA ligase with formation of a recombinant DNA, incubates the recombinant DNA obtained with transformable E. coli cells with transformation of the recombinant DNA into the cells, cultures the transformed cells on a nutrient medium containing raffinose as sole C-source, isolates and lyses the antibiotic-resistant colonies formed, isolates from the lysate the plasmid DNA, splits this with restriction endonuclease Hind III or Eco R I, dilutes the solution obtained and treats with DNA ligase, again introduces the renatured plasmids obtained into transformable cells which constitutively do not form invertase, again cultures the transformed cells on a nutrient medium containing raffinose as C-source, as well as antibiotic, and isolates the colonies formed which do not utilise raffinose.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19813122216 DE3122216A1 (en) | 1981-06-04 | 1981-06-04 | METHOD FOR PRODUCING A MICROORGANISM, WHICH (ALPHA) -GALACTOSIDASE BUT DOES NOT CREATE INVERTASE, MICROORGANISM OBTAINED IN THIS OBTAIN, AND ITS USE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK251682A true DK251682A (en) | 1982-12-05 |
Family
ID=6133914
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK251682A DK251682A (en) | 1981-06-04 | 1982-06-04 | PROCEDURE FOR THE PREPARATION OF A MICRO-ORGANISM FORMING ALFA-GALACTOSIDASE BUT NOT INVERTASE |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5061625A (en) |
| EP (1) | EP0066857B1 (en) |
| JP (1) | JPS57208985A (en) |
| AT (1) | ATE29525T1 (en) |
| AU (1) | AU531972B2 (en) |
| CA (1) | CA1194433A (en) |
| CS (1) | CS272753B2 (en) |
| DD (2) | DD210467A5 (en) |
| DE (2) | DE3122216A1 (en) |
| DK (1) | DK251682A (en) |
| HU (1) | HU193266B (en) |
| SU (1) | SU1431681A3 (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE81141B1 (en) * | 1983-06-24 | 2000-04-05 | Genencor Int | Procaryotic carbonyl hydrolases |
| SU1364343A1 (en) * | 1984-07-13 | 1988-01-07 | Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов | Method of producing manъs leukocytic interferon alfa-2 |
| US5741672A (en) * | 1984-07-27 | 1998-04-21 | Unilever Patent Holdings B.V. | Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes |
| US5240838A (en) * | 1984-07-27 | 1993-08-31 | Internationale Otrool Maatschappij "Octropa" B.V. | Regulatory sequences of alcohol oxidase (MOX) and dihydroxyacetonesynthase (DAS) of Hansenula polymorpha |
| FI861548A0 (en) * | 1986-04-11 | 1986-04-11 | Alko Ab Oy | NYA JAESTSTAMMAR VARI MED GENTEKNISKA FOERFARANDEN INFOERTS EN -GALAKTOSIDASGEN OCH FOERFARANDEN FOER INDUSTRIELLT UTNYTTJANDE AV DYLIKA JAESTSTAMMAR. |
| EP0255153B1 (en) * | 1986-06-03 | 1995-01-18 | Unilever N.V. | Production of guar alpha-galactosidase by hosts transformed by recombinant DNA methods |
| CA1339101C (en) * | 1986-06-03 | 1997-07-29 | Nicolaas Overbeeke | Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods |
| EP0487159A1 (en) * | 1990-11-23 | 1992-05-27 | Unilever N.V. | A food-grade vector suitable for transforming a food-grade host cell, use of said vector for transforming food-grade host cells, and use of said transformed cells in biotransformation processes |
| US6770468B1 (en) | 1999-09-14 | 2004-08-03 | Genzyme Glycobiology Research Institute, Inc. | Phosphodiester-α-GlcNAcase of the lysosomal targeting pathway |
| US6642038B1 (en) | 1999-09-14 | 2003-11-04 | Genzyme Glycobiology Research Institute, Inc. | GlcNAc phosphotransferase of the lysosomal targeting pathway |
| US6800472B2 (en) | 2001-12-21 | 2004-10-05 | Genzyme Glycobiology Research Institute, Inc. | Expression of lysosomal hydrolase in cells expressing pro-N-acetylglucosamine-1-phosphodiester α-N-acetyl glucosimanidase |
| RU2504583C1 (en) * | 2012-10-03 | 2014-01-20 | Федеральное государственное бюджетное учреждение науки Тихоокеанский институт биоорганической химии им. Г.Б. Елякова Дальневосточного отделения Российской академии наук (ТИБОХ ДВО РАН) | PLASMID 40Gal DETERMINING SYNTHESIS OF α-GALACTOSIDASE α-PsGal, STRAIN Ecoli Rosetta(DE3)/40Gal - PRODUCER OF CHIMERIC PROTEIN CONTAINING AMINO-ACID SEQUENCE α-PsGal, AND METHOD FOR ITS PRODUCTION |
| CN115417510B (en) * | 2022-09-20 | 2023-07-04 | 南京农业大学 | Method for removing extracellular antibiotic resistance genes in water |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3957578A (en) * | 1975-01-03 | 1976-05-18 | Hokkaido Sugar Co., Ltd. | Method for manufacture of α-galactosidase by microorganism |
-
1981
- 1981-06-04 DE DE19813122216 patent/DE3122216A1/en not_active Withdrawn
-
1982
- 1982-05-18 CS CS363582A patent/CS272753B2/en unknown
- 1982-05-19 AU AU83826/82A patent/AU531972B2/en not_active Ceased
- 1982-05-27 CA CA000403872A patent/CA1194433A/en not_active Expired
- 1982-06-02 JP JP57093239A patent/JPS57208985A/en active Granted
- 1982-06-02 DD DD82255231A patent/DD210467A5/en not_active IP Right Cessation
- 1982-06-02 DD DD82240391A patent/DD202735A5/en not_active IP Right Cessation
- 1982-06-03 DE DE8282104893T patent/DE3277213D1/en not_active Expired
- 1982-06-03 EP EP82104893A patent/EP0066857B1/en not_active Expired
- 1982-06-03 HU HU821796A patent/HU193266B/en not_active IP Right Cessation
- 1982-06-03 AT AT82104893T patent/ATE29525T1/en not_active IP Right Cessation
- 1982-06-04 DK DK251682A patent/DK251682A/en not_active Application Discontinuation
- 1982-06-04 SU SU823448724A patent/SU1431681A3/en active
-
1988
- 1988-08-24 US US07/235,910 patent/US5061625A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CS363582A2 (en) | 1990-06-13 |
| JPS6251588B2 (en) | 1987-10-30 |
| SU1431681A3 (en) | 1988-10-15 |
| EP0066857A2 (en) | 1982-12-15 |
| CA1194433A (en) | 1985-10-01 |
| EP0066857B1 (en) | 1987-09-09 |
| US5061625A (en) | 1991-10-29 |
| EP0066857A3 (en) | 1983-06-22 |
| AU531972B2 (en) | 1983-09-15 |
| AU8382682A (en) | 1982-12-09 |
| CS272753B2 (en) | 1991-02-12 |
| DE3122216A1 (en) | 1982-12-23 |
| JPS57208985A (en) | 1982-12-22 |
| DD202735A5 (en) | 1983-09-28 |
| DD210467A5 (en) | 1984-06-13 |
| ATE29525T1 (en) | 1987-09-15 |
| HU193266B (en) | 1987-09-28 |
| DE3277213D1 (en) | 1987-10-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| AHB | Application shelved due to non-payment |