DK2486405T3 - Fremgangsmåde til mængdebestemmelse af biomolekyler - Google Patents

Fremgangsmåde til mængdebestemmelse af biomolekyler Download PDF

Info

Publication number
DK2486405T3
DK2486405T3 DK10771054.3T DK10771054T DK2486405T3 DK 2486405 T3 DK2486405 T3 DK 2486405T3 DK 10771054 T DK10771054 T DK 10771054T DK 2486405 T3 DK2486405 T3 DK 2486405T3
Authority
DK
Denmark
Prior art keywords
biomolecules
tissue
cells
proteins
sample
Prior art date
Application number
DK10771054.3T
Other languages
English (en)
Inventor
Matthias Mann
Tamar Geiger
Francesca Forner
Juergen Cox
Jacek R Wisniewski
Original Assignee
Max Planck Gesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft filed Critical Max Planck Gesellschaft
Application granted granted Critical
Publication of DK2486405T3 publication Critical patent/DK2486405T3/da

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (5)

1. Fremgangsmåde til mængdebestemmelse af ét eller et stort antal første biomolekyler i en prøve omfattende: (a) ekstraktion af et stort antal andre biomolekyler omfattende et stort antal referencebiomolekyler fra en blanding af mindst to forskellige cellebestande, idet de to forskellige cellebestande er bestande af (i) celler fra forskellige cellelinier; eller (ii) celler fra forskellige cellekulturer, hvor kulturerne er blevet underkastet forskellige dyrkningsbetingelser, idet hvert af det store antal referencebiomolekyler er en metabolisk isotopmærket form af hvert af det store antal første biomolekyler, idet formen er en enkelt form, og idet den metaboliske isotopmærkning er en mærkning med stabile isotoper med aminosyrer i cellekultur (SILAC), hvorved der fås en standardblanding; (b) blanding af standardblandingen med prøven; (c) bestemmelse af mængden af det store antal første biomolekyler i prøven; og (d) sammenligning af den med mængden af et stort antal referencebiomolekyler blandt et stort antal andre biomolekyler i standardblandingen, idet de nævnte biomolekyler er proteiner eller peptider, og idet mængden af det store antal første biomolekyler og mængden af referencebiomolekyler bestemmes ved massespektrometri.
2. Fremgangsmåde ifølge krav 1, ved hvilken det store antal referencebiomolekyler i standardblandingen er forudvalgt, og den massespektrometriske analyse er målrettet mod dem ved enkelt- eller flerdobbelt ionovervågning (single or multiple ion monitoring).
3. Fremgangsmåde ifølge krav 1 eller 2, ved hvilken sammenligningstrinnet omfatter bestemmelse af intensitetsforholdet mellem toppene fra det store antal første biomolekyler og de tilsvarende toppe fra det store antal referencebiomolekyler.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, ved hvilken mindst to forskellige cellebestande afviger fra hinanden med hensyn til cellemorphologi, ekspressionsprofil af mindst ét biomolekyle, fortrinsvis ekspressionsprofil af et stort antal biomolekyler, og/eller differentiationstilstand.
5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken prøven er valgt fra gruppen af prøver bestående af celler, såsom tumorceller, f.eks. i forbindelse med blod stående tumorceller; helt væv eller udvalgte dele af et væv; sundt væv; væv, der står i forbindelse med en sygdom, såsom kronisk inflammation, stofskiftesygdom, især diabetes, hjertekarsygdom, tumorvæv, f.eks. brystcancervæv, andet carcinomvæv, sarcomvæv, neuroendokrint tumorvæv, i forbindelse med blod stående tumorvæv, lymfomvæv og teratomvæv; subcellulære afdelinger af celler eller væv, f.eks. mitochondrier eller cellekerner; legemsvæsker; ekstrakter af ovenstående; og udvalgte proteinfraktioner fra ovennævnte, f.eks. berigede membranproteiner, glyco-proteiner, phosphorylerede proteiner, acetylerede proteiner, ubiquitinerede proteiner, proteiner med andre modifikationer og proteinkomplekser.
DK10771054.3T 2009-10-06 2010-10-06 Fremgangsmåde til mængdebestemmelse af biomolekyler DK2486405T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24903209P 2009-10-06 2009-10-06
EP09172382A EP2309262A1 (en) 2009-10-06 2009-10-06 Method for quantifying biomolecules
PCT/EP2010/064922 WO2011042467A1 (en) 2009-10-06 2010-10-06 Method for quantifying biomolecules

Publications (1)

Publication Number Publication Date
DK2486405T3 true DK2486405T3 (da) 2014-02-17

Family

ID=41682315

Family Applications (1)

Application Number Title Priority Date Filing Date
DK10771054.3T DK2486405T3 (da) 2009-10-06 2010-10-06 Fremgangsmåde til mængdebestemmelse af biomolekyler

Country Status (5)

Country Link
US (1) US8741556B2 (da)
EP (2) EP2309262A1 (da)
JP (1) JP5789262B2 (da)
DK (1) DK2486405T3 (da)
WO (1) WO2011042467A1 (da)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG186302A1 (en) 2010-06-16 2013-02-28 Abbott Lab Comparison of protein samples
US9405884B2 (en) 2010-06-16 2016-08-02 Abbvie Inc. Methods and systems for the analysis of protein samples
EP2596369A4 (en) * 2010-07-22 2014-01-22 Univ Georgetown MASS SPECTROMETRY METHODS FOR QUANTIFICATION OF NPY 1-36 AND NPY 3-36
EP2934749B1 (en) * 2012-12-19 2023-01-18 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Reaction vessel for sample preparation
WO2014194320A1 (en) * 2013-05-31 2014-12-04 The Uab Research Foundation Chromatography mass spectrometry method and system
WO2015049763A1 (ja) * 2013-10-03 2015-04-09 株式会社島津製作所 質量分析を用いたタンパク質の定量方法
CN103898075A (zh) * 2014-04-19 2014-07-02 吉林大学 新型高活力谷胱甘肽过氧化物酶gpx突变体及其制备方法
CN103881988A (zh) * 2014-04-19 2014-06-25 吉林大学 高活力谷胱甘肽过氧化物酶gpx3突变体及其制备方法
SG10202012249YA (en) * 2014-12-08 2021-01-28 Berg Llc Use of markers including filamin a in the diagnosis and treatment of prostate cancer
EP3091359A1 (de) * 2015-05-08 2016-11-09 Leibniz - Institut für Analytische Wissenschaften - ISAS - E.V. Verfahren zur identifizierung von markerproteinen zur diagnose und risikostratifizierung von störungen der blutgerinnung
CN106324249A (zh) * 2015-07-03 2017-01-11 中国科学院大连化学物理研究所 一种含血清培养液中分泌蛋白的样品预处理方法
CA3002962A1 (en) * 2015-11-05 2017-05-11 Vanderbilt University Protein quantitation in multicellular tissue specimens
US20170168055A1 (en) * 2015-12-11 2017-06-15 Expression Pathology, Inc. SRM/MRM Assays
RU2729116C2 (ru) 2015-12-16 2020-08-04 Гритстоун Онколоджи, Инк. Идентификация, производство и применение неоантигенов
US11204355B2 (en) 2017-02-20 2021-12-21 Vanderbilt University Immune checkpoint molecular fitness profiling by mass spectrometry
KR20200087143A (ko) 2017-10-10 2020-07-20 그릿스톤 온콜로지, 인코포레이티드 핫스팟을 이용한 신생항원 동정
US11885815B2 (en) 2017-11-22 2024-01-30 Gritstone Bio, Inc. Reducing junction epitope presentation for neoantigens
WO2023049541A1 (en) * 2021-07-23 2023-03-30 Cassava Sciences, Inc. Blood-based diagnostic assays for alzheimer's disease

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE536422T1 (de) 1998-08-25 2011-12-15 Univ Washington Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen
CA2359649A1 (en) * 1999-02-16 2000-08-24 Lance A. Liotta Lcm (laser capture microdissection) for cellular protein analysis
US7473535B2 (en) 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
CU23459A1 (es) * 2003-11-19 2009-12-01 Ct Ingenieria Genetica Biotech Método de aislamiento selectivo de péptidos para la identificación y análisis cuantitativo de proteínas en mezclas complejas

Also Published As

Publication number Publication date
US8741556B2 (en) 2014-06-03
EP2309262A1 (en) 2011-04-13
EP2486405A1 (en) 2012-08-15
US20120264154A1 (en) 2012-10-18
JP5789262B2 (ja) 2015-10-07
WO2011042467A1 (en) 2011-04-14
EP2486405B1 (en) 2013-12-11
JP2013506856A (ja) 2013-02-28

Similar Documents

Publication Publication Date Title
DK2486405T3 (da) Fremgangsmåde til mængdebestemmelse af biomolekyler
Rozanova et al. Quantitative mass spectrometry-based proteomics: an overview
Cifani et al. Towards comprehensive and quantitative proteomics for diagnosis and therapy of human disease
Macklin et al. Recent advances in mass spectrometry based clinical proteomics: applications to cancer research
Ebhardt et al. Applications of targeted proteomics in systems biology and translational medicine
Selevsek et al. Systematic quantification of peptides/proteins in urine using selected reaction monitoring
Liang et al. Quantitative proteomics for cancer biomarker discovery
Maes et al. Proteomics in cancer research: Are we ready for clinical practice?
Simpson et al. Quantitative mass spectrometry-based techniques for clinical use: biomarker identification and quantification
Mardamshina et al. Next-generation proteomics and its application to clinical breast cancer research
MacMullan et al. Quantitative proteomics and metabolomics reveal biomarkers of disease as potential immunotherapy targets and indicators of therapeutic efficacy
Khoo et al. Proteomic discovery of non-invasive biomarkers of localized prostate cancer using mass spectrometry
Thomas et al. Glycoproteomics: growing up fast
AU2015284050A1 (en) SRM assays to chemotherapy targets
He et al. Antibody-independent targeted quantification of TMPRSS2-ERG fusion protein products in prostate cancer
Nigjeh et al. Spectral library‐based glycopeptide analysis–detection of circulating galectin‐3 binding protein in pancreatic cancer
Fugmann et al. DeepQuanTR: MALDI‐MS‐based label‐free quantification of proteins in complex biological samples
Xu Combining laser capture microdissection and proteomics: methodologies and clinical applications
Dudley et al. Phosphoproteomic techniques and applications
KR101541958B1 (ko) 성체줄기세포의 노화 검출용 마커 및 그 용도
Parker et al. Emerging proteomic technologies for elucidating context‐dependent cellular signaling events: A big challenge of tiny proportions
Chaube Absolute quantitation of post-translational modifications
Hjelle et al. Clinical proteomics of myeloid leukemia
Timms et al. Overview of quantitative LC-MS techniques for proteomics and activitomics
JP6742034B2 (ja) 多細胞組織検体におけるタンパク質の定量