DK2437869T3 - Multiplexanalyse af stacked transgenic protein - Google Patents
Multiplexanalyse af stacked transgenic protein Download PDFInfo
- Publication number
- DK2437869T3 DK2437869T3 DK10784066.2T DK10784066T DK2437869T3 DK 2437869 T3 DK2437869 T3 DK 2437869T3 DK 10784066 T DK10784066 T DK 10784066T DK 2437869 T3 DK2437869 T3 DK 2437869T3
- Authority
- DK
- Denmark
- Prior art keywords
- proteins
- protein
- plant
- multiplex
- interest
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (2)
1. Fremgangsmåde med højt udbytte til detektering af forekomsten af to eller flere proteiner af interesse med kendte aminosyresekvenser i en plantebaseret prøve fra en transgen plante, hvilken fremgangsmåde omfatter: i) tilvejebringelse af massespektraldata for to eller flere proteiner af interesse, der er forventede produkter af transgen ekspression i den transgene plante; ii) tilvejebringelse af en første injektion af en kompleks plantebaseret prøve indeholdende proteiner, hvor prøven er en rå plantematrix ekstraheret fra væv af interesse; iii) etablering af kontakt mellem den rå plantematrix og en protease for at fordøje proteiner til peptider; iv) injektion af den fordøjede rå plantematrix i en LC-MS-anordning; v) opnåelse af samtidige massespektraldata for peptiderne; vi) sammenligning af de samtidige massespektraldata fra v) med massespektraldataene tilvejebragt til de to eller flere proteiner af interesse, hvorved forekomst eller fravær af de to eller flere proteiner af interesse bestemmes.
2. Fremgangsmåde ifølge krav 1, hvor de to eller flere proteiner af interesse er to proteiner af interesse eller fire proteiner af interesse.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18377709P | 2009-06-03 | 2009-06-03 | |
| PCT/US2010/037192 WO2010141674A2 (en) | 2009-06-03 | 2010-06-03 | Multiplex analysis of stacked transgenic protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK2437869T3 true DK2437869T3 (da) | 2015-08-10 |
| DK2437869T4 DK2437869T4 (da) | 2023-06-06 |
Family
ID=43298498
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10784066.2T DK2437869T4 (da) | 2009-06-03 | 2010-06-03 | Multiplexanalyse af stakket transgent protein |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US8227252B2 (da) |
| EP (1) | EP2437869B2 (da) |
| CN (2) | CN105241972B (da) |
| AU (1) | AU2010256590B2 (da) |
| BR (1) | BRPI1010781B1 (da) |
| CA (2) | CA2999632A1 (da) |
| CL (1) | CL2011003063A1 (da) |
| CO (1) | CO6430443A2 (da) |
| DK (1) | DK2437869T4 (da) |
| ES (1) | ES2544706T5 (da) |
| MX (1) | MX2011012942A (da) |
| NZ (1) | NZ596287A (da) |
| PL (1) | PL2437869T5 (da) |
| RU (1) | RU2561458C2 (da) |
| UA (1) | UA112515C2 (da) |
| WO (1) | WO2010141674A2 (da) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA112515C2 (uk) | 2009-06-03 | 2016-09-26 | Дау Аґросаєнсиз Елелсі | Спосіб виявлення присутності двох або більше білків, що становлять інтерес, в зразках рослинного походження |
| US8835361B2 (en) | 2010-06-01 | 2014-09-16 | The Curators Of The University Of Missouri | High-throughput quantitation of crop seed proteins |
| US9933416B1 (en) | 2013-07-30 | 2018-04-03 | Pioneer Hi-Bred International, Inc. | Detection and quantification of polypeptides in plants without a reference standard by mass spectrometry |
| US20150355189A1 (en) * | 2014-06-10 | 2015-12-10 | Dow Agrosciences Llc | Quantitative analysis of transgenic protein 5-enolpyruvylshikimate-3-phosphate synthase |
| WO2020198688A1 (en) * | 2019-03-28 | 2020-10-01 | The Regents Of The University Of California | Concurrent analysis of multiple analytes |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030167512A1 (en) * | 1998-01-16 | 2003-09-04 | Kumagai Monto H. | Method of determining the presence of a trait in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in a positive orientation |
| US7038113B1 (en) * | 1999-03-08 | 2006-05-02 | The Samuel Roberts Noble Foundation, Inc. | Genetic manipulation of isoflavonoids |
| US7417178B2 (en) * | 2000-05-02 | 2008-08-26 | Ventria Bioscience | Expression of human milk proteins in transgenic plants |
| AU2001255797A1 (en) * | 2000-05-02 | 2001-11-12 | Applied Phytologics, Inc. | Enhanced gene expression in plants using transcription factors |
| CA2444524A1 (en) * | 2001-04-17 | 2002-10-24 | Ista, S.P.A. | Methods for mass spectrometry detection and quantification of specific target proteins in complex biological samples |
| RU2242764C1 (ru) * | 2003-08-07 | 2004-12-20 | Институт цитологии Российской академии наук (статус государственного учреждения) | Способ определения концентрации основного белка теплового шока 70 кда |
| EP1787125A4 (en) | 2004-07-29 | 2008-10-29 | Eisai R&D Man Co Ltd | ABSOLUTE QUANTIFICATION OF PROTEIN CONTENT BASED ON ABUNDANCE INDEX OF EXPONENTIALLY MODIFIED PROTEINS BY MASS SPECTROMETRY |
| EP1941280A2 (en) | 2005-10-13 | 2008-07-09 | Applera Corporation | Methods for the development of a biomolecule assay |
| CN101055267A (zh) * | 2006-04-13 | 2007-10-17 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | 一种用于快速鉴定蛋白质及其n端序列的新方法和试剂盒 |
| WO2007132164A2 (en) * | 2006-05-02 | 2007-11-22 | Royal Holloway And Bedford New College | Analysis of proteins |
| CN1944648B (zh) * | 2006-10-19 | 2010-06-30 | 杭州市农业科学研究院 | 一种提高植物耐盐性的水稻Cyp2基因 |
| CN101423550B (zh) * | 2007-10-30 | 2012-11-21 | 武汉大学 | 基因工程免疫抑制多肽及制备方法和用途 |
| UA112515C2 (uk) | 2009-06-03 | 2016-09-26 | Дау Аґросаєнсиз Елелсі | Спосіб виявлення присутності двох або більше білків, що становлять інтерес, в зразках рослинного походження |
-
2010
- 2010-03-06 UA UAA201115616A patent/UA112515C2/uk unknown
- 2010-06-03 CA CA2999632A patent/CA2999632A1/en not_active Abandoned
- 2010-06-03 PL PL10784066.2T patent/PL2437869T5/pl unknown
- 2010-06-03 WO PCT/US2010/037192 patent/WO2010141674A2/en active Application Filing
- 2010-06-03 DK DK10784066.2T patent/DK2437869T4/da active
- 2010-06-03 EP EP10784066.2A patent/EP2437869B2/en active Active
- 2010-06-03 BR BRPI1010781-9A patent/BRPI1010781B1/pt not_active IP Right Cessation
- 2010-06-03 CN CN201510591882.8A patent/CN105241972B/zh active Active
- 2010-06-03 AU AU2010256590A patent/AU2010256590B2/en active Active
- 2010-06-03 US US12/793,058 patent/US8227252B2/en active Active
- 2010-06-03 CN CN201080024557.9A patent/CN102458617B/zh active Active
- 2010-06-03 RU RU2011154371/10A patent/RU2561458C2/ru active
- 2010-06-03 MX MX2011012942A patent/MX2011012942A/es active IP Right Grant
- 2010-06-03 CA CA2763896A patent/CA2763896C/en active Active
- 2010-06-03 ES ES10784066T patent/ES2544706T5/es active Active
- 2010-06-03 NZ NZ596287A patent/NZ596287A/xx unknown
-
2011
- 2011-12-02 CL CL2011003063A patent/CL2011003063A1/es unknown
-
2012
- 2012-01-03 CO CO12000507A patent/CO6430443A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN102458617B (zh) | 2015-10-14 |
| MX2011012942A (es) | 2012-01-31 |
| CA2999632A1 (en) | 2010-12-09 |
| EP2437869A2 (en) | 2012-04-11 |
| PL2437869T3 (pl) | 2015-12-31 |
| RU2011154371A (ru) | 2013-07-20 |
| NZ596287A (en) | 2013-11-29 |
| BRPI1010781B1 (pt) | 2022-05-24 |
| UA112515C2 (uk) | 2016-09-26 |
| ES2544706T5 (es) | 2023-07-19 |
| CO6430443A2 (es) | 2012-04-30 |
| BRPI1010781A2 (pt) | 2016-03-22 |
| EP2437869B2 (en) | 2023-05-03 |
| CA2763896C (en) | 2018-05-01 |
| EP2437869B1 (en) | 2015-07-22 |
| ES2544706T3 (es) | 2015-09-03 |
| DK2437869T4 (da) | 2023-06-06 |
| CA2763896A1 (en) | 2010-12-09 |
| CN105241972A (zh) | 2016-01-13 |
| CL2011003063A1 (es) | 2012-07-20 |
| AU2010256590A1 (en) | 2012-01-19 |
| CN105241972B (zh) | 2017-09-08 |
| WO2010141674A3 (en) | 2011-03-17 |
| PL2437869T5 (pl) | 2023-07-03 |
| AU2010256590B2 (en) | 2015-05-21 |
| RU2561458C2 (ru) | 2015-08-27 |
| EP2437869A4 (en) | 2013-03-06 |
| CN102458617A (zh) | 2012-05-16 |
| US8227252B2 (en) | 2012-07-24 |
| WO2010141674A2 (en) | 2010-12-09 |
| US20100313290A1 (en) | 2010-12-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2015275086B2 (en) | Quantitative analysis of transgenic proteins | |
| Newton et al. | Plant proteome analysis by mass spectrometry: principles, problems, pitfalls and recent developments | |
| US20240019446A1 (en) | Methods and systems for selective quantitation and detection of allergens including gly m 7 | |
| DK2437869T3 (da) | Multiplexanalyse af stacked transgenic protein | |
| AU2015301806A1 (en) | Methods and systems for selective quantitation and detection of allergens | |
| US20190128896A1 (en) | Methods and systems for selective quantitation and detection of allergens | |
| KR20210052477A (ko) | 단백질 검출을 위한 조성물 및 방법 | |
| Christofakis et al. | LC–MS/MS techniques for food allergen testing |