DK2356218T3 - Fremgangsmåde og medium til neural differentiering af pluripotente celler - Google Patents
Fremgangsmåde og medium til neural differentiering af pluripotente celler Download PDFInfo
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- DK2356218T3 DK2356218T3 DK09764840.6T DK09764840T DK2356218T3 DK 2356218 T3 DK2356218 T3 DK 2356218T3 DK 09764840 T DK09764840 T DK 09764840T DK 2356218 T3 DK2356218 T3 DK 2356218T3
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/0618—Cells of the nervous system
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- A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
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- A61P25/00—Drugs for disorders of the nervous system
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- C12N5/0602—Vertebrate cells
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- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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Claims (12)
1. Kulturmedium omfattende: a) noggin eller fragmenter deraf, der inhiberer BMP-signalvejen; og b) en inhibitor af Transforming Growth Factor (TGF)/aktivin/nodal-signalvejen valgt fra gruppen bestående af SB431542, Lefty-A og Cerberus og fragmenter af Lefty-A og Cerberus, der inhiberer TGF/aktivin/nodal-signalvejen.
2. Kulturmedium ifølge krav 1, hvor kulturmediet yderligere omfatter : a) en kulstofkilde; b) essentielle aminosyrer; c) vitaminer: d) en purin og en pyrimidin; e) uorganiske salte; f) selenium; g) en antioxidant; h) en phospholipid prækursor; i) en unik fedtsyre; og j) et bærerprotein.
3. In vitro-fremgangsmåde til fremstilling afen population af neurale prækursorer, hvor fremgangsmåden omfatter trinnet at dyrke pluripotente celler med kulturmediet som defineret i et hvilket som helst af kravene 1 til 2.
4. In v/fro-fremgangsmåde ifølge krav 3, hvor de pluripotente celler er humane pluripotente celler.
5. In v/fro-fremgangsmåde ifølge krav 3 eller 4, hvor de pluripotente celler er stamceller.
6. In v/'tro-fremgangsmåde ifølge krav 5, hvor stamcellerne er embryonale stamceller.
7. In v/fro-fremgangsmåde ifølge krav 3 eller 4, hvor de pluripotente celler er inducerede pluripotente celler (IPS).
8. Kit til dyrkning af celler omfattende: a) noggin eller fragmenter deraf, der inhiberer BMP-signalvejen og b) en inhibitor af Transforming Growth Factor (TGF)/aktivin/nodal-signalvejen valgt fra gruppen bestående af SB431542, Lefty-A og Cerberus og fragmenter af Lefty-A og Cerberus, der inhiberer TGF/aktivin/nodal-signalvejen.
9. Kit til dyrkning af celler ifølge krav 8, yderligere omfattende en mediumbase.
10. In v/'fro-fremgangsmåde ifølge et hvilket som helst af kravene 3-7, hvor de pluripotente celler dyrkes med mediet som defineret i krav 1 eller 2 i mindst 5 dage.
11. In Wfro-fremgangsmåde ifølge et hvilket som helst af kravene 3-7, hvor de pluripotente celler dyrkes med mediet som defineret i krav 1 eller 2 i mindst 7 dage.
12. In vitro-fremgangsmåde ifølge et hvilket som helst af kravene 3-7, hvor de pluripotente celler dyrkes med mediet som defineret i krav 1 eller 2 i mindst 10 dage.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP08305887 | 2008-12-05 | ||
PCT/EP2009/066504 WO2010063848A1 (en) | 2008-12-05 | 2009-12-07 | Method and medium for neural differentiation of pluripotent cells |
Publications (1)
Publication Number | Publication Date |
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DK2356218T3 true DK2356218T3 (da) | 2017-08-21 |
Family
ID=40427840
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK09764840.6T DK2356218T3 (da) | 2008-12-05 | 2009-12-07 | Fremgangsmåde og medium til neural differentiering af pluripotente celler |
Country Status (6)
Country | Link |
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US (3) | US20110229441A1 (da) |
EP (1) | EP2356218B1 (da) |
JP (1) | JP5604766B2 (da) |
DK (1) | DK2356218T3 (da) |
ES (1) | ES2633935T3 (da) |
WO (1) | WO2010063848A1 (da) |
Families Citing this family (48)
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EP2610336A1 (en) | 2007-07-31 | 2013-07-03 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
US9062290B2 (en) | 2007-11-27 | 2015-06-23 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
KR20190057164A (ko) | 2008-02-21 | 2019-05-27 | 얀센 바이오테크 인코포레이티드 | 세포 부착, 배양 및 탈리를 위한 방법, 표면 개질 플레이트 및 조성물 |
CA2729121C (en) | 2008-06-30 | 2019-04-09 | Centocor Ortho Biotech Inc. | Differentiation of pluripotent stem cells |
CA2742268C (en) | 2008-10-31 | 2020-02-18 | Centocor Ortho Biotech Inc. | Differentiation of human embryonic stem cells to the pancreatic endocrine lineage |
MX2011004563A (es) | 2008-10-31 | 2011-06-01 | Centocor Ortho Biotech Inc | Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico. |
CA2744227C (en) | 2008-11-20 | 2018-10-02 | Centocor Ortho Biotech Inc. | Methods and compositions for cell attachment and cultivation on planar substrates |
KR101837080B1 (ko) | 2008-11-20 | 2018-03-09 | 얀센 바이오테크 인코포레이티드 | 마이크로-캐리어 상의 만능 줄기 세포 배양 |
US8642334B2 (en) | 2009-02-17 | 2014-02-04 | Memorial Sloan Kettering Cancer Center | Methods of neural conversion of human embryonic stem cells |
KR102058901B1 (ko) | 2009-07-20 | 2019-12-24 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
US11261425B2 (en) | 2009-08-12 | 2022-03-01 | Kyoto University | Method for inducing differentiation of pluripotent stem cells into neural precursor cells |
US10119120B2 (en) | 2009-08-12 | 2018-11-06 | Kyoto University | Method for inducing differentiation of pluripotent stem cells into neural precursor cells |
KR101168053B1 (ko) * | 2009-11-06 | 2012-07-24 | 연세대학교 산학협력단 | 효율적이고 보편적인 전분화능 줄기세포의 신경세포 분화 유도방법 |
WO2011079017A2 (en) | 2009-12-23 | 2011-06-30 | Centocor Ortho Biotech Inc. | Differentiation of human embryonic stem cells |
EP2542667A4 (en) | 2010-03-01 | 2013-07-31 | Janssen Biotech Inc | PROCESS FOR CLEANING CELLS OBTAINED FROM PLURIPOTENTIAL STEM CELLS |
KR101903562B1 (ko) | 2010-05-12 | 2018-10-02 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
EP2412724A1 (en) * | 2010-07-29 | 2012-02-01 | Centre National de la Recherche Scientifique (C.N.R.S) | Regulation of Glypican 4 activity to modulate the fate of stem cells and uses thereof |
US20130149287A1 (en) | 2010-07-30 | 2013-06-13 | Cambridge Enterprise Limited | Corticogenesis of Human Pluripotent Cells |
US9181528B2 (en) | 2010-08-31 | 2015-11-10 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
EP2611909B1 (en) | 2010-08-31 | 2018-01-17 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
CN103154239B (zh) | 2010-08-31 | 2018-05-15 | 詹森生物科技公司 | 人胚胎干细胞的分化 |
US9181529B2 (en) | 2010-10-19 | 2015-11-10 | Cellular Dynamics International, Inc. | Titration of differentiation medium components |
DK2694644T3 (da) | 2011-03-30 | 2018-04-16 | Cellular Dynamics Int Inc | Priming af pluripotente stamceller til neural differentiering |
US10280398B2 (en) | 2011-11-04 | 2019-05-07 | Memorial Sloan-Kettering Cancer Center | Midbrain dopamine (DA) neurons for engraftment |
CN105143446B (zh) | 2011-12-22 | 2020-11-03 | 詹森生物科技公司 | 人胚胎干细胞分化成单一激素胰岛素阳性细胞 |
CN102604894B (zh) * | 2012-02-29 | 2014-07-30 | 中国科学院广州生物医药与健康研究院 | 用于制备神经干细胞的培养基及其用途 |
CN104160018A (zh) | 2012-03-07 | 2014-11-19 | 詹森生物科技公司 | 用于扩增和维持多能干细胞的成分确定的培养基 |
US20150064734A1 (en) * | 2012-03-21 | 2015-03-05 | Kyoto University | Method for screening therapeutic and/or prophylactic agents for alzheimer's disease |
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WO2013187416A1 (ja) * | 2012-06-12 | 2013-12-19 | 学校法人 慶應義塾 | 神経系分化に適したiPS細胞の増幅方法、及び神経幹細胞の誘導方法 |
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US10138465B2 (en) | 2012-12-31 | 2018-11-27 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into pancreatic endocrine cells using HB9 regulators |
DK2938724T3 (da) | 2012-12-31 | 2020-12-14 | Janssen Biotech Inc | Dyrkning af humane embryonale stamceller ved luft-væskegrænsefladen til differentiering til endokrine pankreasceller |
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EP2986716A1 (en) * | 2013-04-15 | 2016-02-24 | Institut National de la Santé et de la Recherche Médicale (INSERM) | Method and medium for amplifying neural precursor cells |
AU2014256876B2 (en) | 2013-04-26 | 2020-11-12 | Cornell, University | Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells |
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CN107208045B (zh) | 2014-11-25 | 2018-11-02 | 宾州研究基金会 | 人神经胶质细胞转化成用于大脑和脊髓修复的神经细胞的化学重编程 |
SG11201707857VA (en) * | 2015-03-30 | 2017-10-30 | Ajinomoto Kk | Chelated iron-containing culture medium for neural stem cells |
MA45479A (fr) | 2016-04-14 | 2019-02-20 | Janssen Biotech Inc | Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen |
EP4328301A3 (en) | 2016-08-16 | 2024-06-19 | FUJIFILM Cellular Dynamics, Inc. | Methods for differentiating pluripotent cells |
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Publication number | Priority date | Publication date | Assignee | Title |
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WO2003104444A1 (en) * | 2002-06-05 | 2003-12-18 | Es Cell International Pte Ltd | Generation of neural stem cells from undifferentiated human embryonic stem cells |
US20080089868A1 (en) * | 2006-04-27 | 2008-04-17 | The Research Foundation Of State University Of New York | Retinal stem cell compositions and methods for preparing and using same |
GB0622394D0 (en) * | 2006-11-09 | 2006-12-20 | Univ Cambridge Tech | Differentiation of pluripotent cells |
US8338170B2 (en) * | 2008-04-21 | 2012-12-25 | Viacyte, Inc. | Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells |
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2009
- 2009-12-07 EP EP09764840.6A patent/EP2356218B1/en active Active
- 2009-12-07 DK DK09764840.6T patent/DK2356218T3/da active
- 2009-12-07 JP JP2011539048A patent/JP5604766B2/ja active Active
- 2009-12-07 US US12/994,980 patent/US20110229441A1/en not_active Abandoned
- 2009-12-07 ES ES09764840.6T patent/ES2633935T3/es active Active
- 2009-12-07 WO PCT/EP2009/066504 patent/WO2010063848A1/en active Application Filing
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2016
- 2016-01-19 US US15/000,528 patent/US20160160177A1/en not_active Abandoned
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2018
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Also Published As
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US20110229441A1 (en) | 2011-09-22 |
ES2633935T3 (es) | 2017-09-26 |
US20180265843A1 (en) | 2018-09-20 |
EP2356218B1 (en) | 2017-05-03 |
EP2356218A1 (en) | 2011-08-17 |
US20160160177A1 (en) | 2016-06-09 |
WO2010063848A1 (en) | 2010-06-10 |
JP2012510805A (ja) | 2012-05-17 |
JP5604766B2 (ja) | 2014-10-15 |
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