DK2356218T3 - Fremgangsmåde og medium til neural differentiering af pluripotente celler - Google Patents

Fremgangsmåde og medium til neural differentiering af pluripotente celler Download PDF

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DK2356218T3
DK2356218T3 DK09764840.6T DK09764840T DK2356218T3 DK 2356218 T3 DK2356218 T3 DK 2356218T3 DK 09764840 T DK09764840 T DK 09764840T DK 2356218 T3 DK2356218 T3 DK 2356218T3
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cells
neural
medium
signaling pathway
pluripotent cells
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Alexandra Benchoua
Anselme Perrier
Laetitia Aubry
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Inserm (Institut Nat De La Santé Et De La Rech Médicale)
Ass Francaise Contre Les Myopathies
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Claims (12)

1. Kulturmedium omfattende: a) noggin eller fragmenter deraf, der inhiberer BMP-signalvejen; og b) en inhibitor af Transforming Growth Factor (TGF)/aktivin/nodal-signalvejen valgt fra gruppen bestående af SB431542, Lefty-A og Cerberus og fragmenter af Lefty-A og Cerberus, der inhiberer TGF/aktivin/nodal-signalvejen.
2. Kulturmedium ifølge krav 1, hvor kulturmediet yderligere omfatter : a) en kulstofkilde; b) essentielle aminosyrer; c) vitaminer: d) en purin og en pyrimidin; e) uorganiske salte; f) selenium; g) en antioxidant; h) en phospholipid prækursor; i) en unik fedtsyre; og j) et bærerprotein.
3. In vitro-fremgangsmåde til fremstilling afen population af neurale prækursorer, hvor fremgangsmåden omfatter trinnet at dyrke pluripotente celler med kulturmediet som defineret i et hvilket som helst af kravene 1 til 2.
4. In v/fro-fremgangsmåde ifølge krav 3, hvor de pluripotente celler er humane pluripotente celler.
5. In v/fro-fremgangsmåde ifølge krav 3 eller 4, hvor de pluripotente celler er stamceller.
6. In v/'tro-fremgangsmåde ifølge krav 5, hvor stamcellerne er embryonale stamceller.
7. In v/fro-fremgangsmåde ifølge krav 3 eller 4, hvor de pluripotente celler er inducerede pluripotente celler (IPS).
8. Kit til dyrkning af celler omfattende: a) noggin eller fragmenter deraf, der inhiberer BMP-signalvejen og b) en inhibitor af Transforming Growth Factor (TGF)/aktivin/nodal-signalvejen valgt fra gruppen bestående af SB431542, Lefty-A og Cerberus og fragmenter af Lefty-A og Cerberus, der inhiberer TGF/aktivin/nodal-signalvejen.
9. Kit til dyrkning af celler ifølge krav 8, yderligere omfattende en mediumbase.
10. In v/'fro-fremgangsmåde ifølge et hvilket som helst af kravene 3-7, hvor de pluripotente celler dyrkes med mediet som defineret i krav 1 eller 2 i mindst 5 dage.
11. In Wfro-fremgangsmåde ifølge et hvilket som helst af kravene 3-7, hvor de pluripotente celler dyrkes med mediet som defineret i krav 1 eller 2 i mindst 7 dage.
12. In vitro-fremgangsmåde ifølge et hvilket som helst af kravene 3-7, hvor de pluripotente celler dyrkes med mediet som defineret i krav 1 eller 2 i mindst 10 dage.
DK09764840.6T 2008-12-05 2009-12-07 Fremgangsmåde og medium til neural differentiering af pluripotente celler DK2356218T3 (da)

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EP08305887 2008-12-05
PCT/EP2009/066504 WO2010063848A1 (en) 2008-12-05 2009-12-07 Method and medium for neural differentiation of pluripotent cells

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EP (1) EP2356218B1 (da)
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DK (1) DK2356218T3 (da)
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