DK2297321T3 - Fremgangsmåde til tilvejebringelse af et variant- bibliotek af DNA-skevenser - Google Patents
Fremgangsmåde til tilvejebringelse af et variant- bibliotek af DNA-skevenserInfo
- Publication number
- DK2297321T3 DK2297321T3 DK09757275.4T DK09757275T DK2297321T3 DK 2297321 T3 DK2297321 T3 DK 2297321T3 DK 09757275 T DK09757275 T DK 09757275T DK 2297321 T3 DK2297321 T3 DK 2297321T3
- Authority
- DK
- Denmark
- Prior art keywords
- providing
- dna sequences
- variant library
- library
- variant
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08010256A EP2130918A1 (de) | 2008-06-05 | 2008-06-05 | Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen |
| PCT/EP2009/003945 WO2009146892A1 (de) | 2008-06-05 | 2009-06-03 | Verfahren zur erzeugung einer varianten-bibliothek von dna-sequenzen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2297321T3 true DK2297321T3 (da) | 2013-04-08 |
Family
ID=39803139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09757275.4T DK2297321T3 (da) | 2008-06-05 | 2009-06-03 | Fremgangsmåde til tilvejebringelse af et variant- bibliotek af DNA-skevenser |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US9856470B2 (da) |
| EP (2) | EP2130918A1 (da) |
| DK (1) | DK2297321T3 (da) |
| WO (1) | WO2009146892A1 (da) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090312196A1 (en) * | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| EP3359669B1 (en) | 2015-10-06 | 2020-05-13 | Pierce Biotechnology, Inc. | Devices and methods for producing proteins |
| US20200347424A1 (en) | 2016-03-08 | 2020-11-05 | Pfeifer & Langen GmbH & Co. KG | Saccharose phosphorylase |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5556747A (en) * | 1990-07-09 | 1996-09-17 | E. R. Squibb & Sons, Inc. | Method for site-directed mutagenesis |
| JP2000512142A (ja) * | 1996-06-07 | 2000-09-19 | マサチューセッツ インスティチュート オブ テクノロジー | プログラムされた連続的変異誘発 |
| GB9701425D0 (en) * | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| GB9712512D0 (en) * | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| US20020048772A1 (en) * | 2000-02-10 | 2002-04-25 | Dahiyat Bassil I. | Protein design automation for protein libraries |
| US6251604B1 (en) * | 1999-08-13 | 2001-06-26 | Genopsys, Inc. | Random mutagenesis and amplification of nucleic acid |
| US6582914B1 (en) * | 2000-10-26 | 2003-06-24 | Genencor International, Inc. | Method for generating a library of oligonucleotides comprising a controlled distribution of mutations |
| DK1409667T4 (da) * | 2001-07-23 | 2009-09-07 | Dsm Ip Assets Bv | Fremgangsm der til at fremstilling varierende polynucleotider |
| GB0213816D0 (en) | 2002-06-14 | 2002-07-24 | Univ Aston | Method of producing DNA and protein libraries |
| US20060141626A1 (en) * | 2004-10-27 | 2006-06-29 | Monsanto Technology Llc | Non-random method of gene shuffling |
-
2008
- 2008-06-05 EP EP08010256A patent/EP2130918A1/de not_active Withdrawn
-
2009
- 2009-06-03 EP EP09757275A patent/EP2297321B1/de active Active
- 2009-06-03 WO PCT/EP2009/003945 patent/WO2009146892A1/de active Application Filing
- 2009-06-03 DK DK09757275.4T patent/DK2297321T3/da active
-
2010
- 2010-12-03 US US12/960,061 patent/US9856470B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2130918A1 (de) | 2009-12-09 |
| US20110152126A1 (en) | 2011-06-23 |
| EP2297321B1 (de) | 2013-01-09 |
| EP2297321A1 (de) | 2011-03-23 |
| US9856470B2 (en) | 2018-01-02 |
| WO2009146892A1 (de) | 2009-12-10 |
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