DK2004819T3 - Filamentøse svampe med reduceret indhold af udp-galactofuranose - Google Patents

Filamentøse svampe med reduceret indhold af udp-galactofuranose Download PDF

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Publication number
DK2004819T3
DK2004819T3 DK07754572.1T DK07754572T DK2004819T3 DK 2004819 T3 DK2004819 T3 DK 2004819T3 DK 07754572 T DK07754572 T DK 07754572T DK 2004819 T3 DK2004819 T3 DK 2004819T3
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Denmark
Prior art keywords
cell
protein
udp
gene
fungal cell
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DK07754572.1T
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English (en)
Inventor
Michael Ward
Wouter Vervecken
Roland Contreras
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Danisco Us Inc
Univ Ghent
Flanders Interuniversity Inst For Biotechnology Vzw
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Application filed by Danisco Us Inc, Univ Ghent, Flanders Interuniversity Inst For Biotechnology Vzw filed Critical Danisco Us Inc
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Publication of DK2004819T3 publication Critical patent/DK2004819T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01024—Alpha-mannosidase (3.2.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99009—UDP-galactopyranose mutase (5.4.99.9)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (16)

  1. FILAMENTØSE SVAMPE MED REDUCERET INDHOLD AF UDP-GALACTOFURANOSE PATENTKRAV
    1. Rekombinant filamentøs svampecelle omfattende et nukleært genom omfattende et inaktiveret UDP-galactopyranosemutase- (UDP-gal/vmutase) gen, hvor svampecellen endvidere omfatter en rekombinant nukleinsyre til ekspression af et sekreteret protein i cellen.
  2. 2. Svampecelle ifølge krav 1, hvor UDP-galP-mutasegenet indeholder en deletion, en insertion eller en omfordeling.
  3. 3. Svampecelle ifølge krav 1, hvor svampecellen er en Aspergillus-celle.
  4. 4. Svampecelle ifølge krav 1, hvor svampecellen er en Aspergillus m'ger-celle eller en Aspergillus oryzae-celle.
  5. 5. Svampecelle ifølge krav 1, hvor det sekreterede protein er glycosyleret protein.
  6. 6. Svampecelle ifølge krav 1, hvor det sekreterede protein er heterologt med cellen.
  7. 7. Svampecelle ifølge krav 1, hvor det sekreterede protein er nativt i forhold til cellen.
  8. 8. Svampecelle ifølge krav 1, hvor det sekreterede protein er et terapeutisk protein, et antistofprotein eller et enzym.
  9. 9. Svampecelle ifølge krav 8, hvor enzymet er en amylase, protease, cellulase, xylanase eller phytase.
  10. 10. Svampecelle ifølge krav 1, hvor det nukleære genom omfatter mindst to inaktiverede UDP-galactopyranosemutasegener.
  11. 11. Svampecelle ifølge krav 1, hvor svampecellen endvidere omfatter en rekombinant nukleinsyre til ekspression af en mannosidase.
  12. 12. Fremgangsmåde til produktion af sekreret protein kodet for af den rekombinante nukleinsyre, hvilken fremgangsmåde omfatter dyrkning af cellen ifølge et hvilket som helst af kravene 1 til 11 i et dyrkningsmedium under forhold, der er egnede til at producere det sekreterede protein.
  13. 13. Fremgangsmåde ifølge krav 12, hvilken fremgangsmåde endvidere omfatter genvinding af det sekreterede protein fra dyrkningsmediet.
  14. 14. Fremgangsmåde til frembringelse af cellen ifølge 1, hvilken fremgangsmåde omfatter a) indføring af en rekombinant nukleinsyre i en filamentøs svampecelle, således at den rekombinante nukleinsyre rekombineres med UDP-galactopyranosemutasegenet af cellens genom, hvorved genet inaktiveres; og b) indføring af en anden rekombinant nukleinsyre i cellen, der tilvejebringer ekspression af det sekreterede protein.
  15. 15. Fremgangsmåde ifølge krav 14, hvor nukleinsyren indføres i UDP-galactopyranosemutasegenet.
  16. 16. Fremgangsmåde ifølge krav 14, hvor nukleinsyren deleterer mindst en del af UDP-galactopyranosemutasegenet.
DK07754572.1T 2006-04-05 2007-04-03 Filamentøse svampe med reduceret indhold af udp-galactofuranose DK2004819T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78931706P 2006-04-05 2006-04-05
PCT/US2007/008067 WO2007123801A1 (en) 2006-04-05 2007-04-03 Filamentous fungi having reduced udp-galactofuranose content

Publications (1)

Publication Number Publication Date
DK2004819T3 true DK2004819T3 (da) 2015-10-05

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Application Number Title Priority Date Filing Date
DK07754572.1T DK2004819T3 (da) 2006-04-05 2007-04-03 Filamentøse svampe med reduceret indhold af udp-galactofuranose

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US (2) US20110104750A1 (da)
EP (1) EP2004819B1 (da)
DK (1) DK2004819T3 (da)
WO (1) WO2007123801A1 (da)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115725559A (zh) * 2022-10-25 2023-03-03 江苏省农业科学院 一种细胞壁合成相关蛋白ugma及其编码基因与应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992010581A1 (en) * 1990-12-10 1992-06-25 Genencor International, Inc. IMPROVED SACCHARIFICATION OF CELLULOSE BY CLONING AND AMPLIFICATION OF THE β-GLUCOSIDASE GENE OF TRICHODERMA REESEI
ATE256747T1 (de) * 1995-05-18 2004-01-15 Genencor Int Glykosyltransferase expression in aspergillus
KR100618495B1 (ko) * 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
WO2001012792A1 (en) * 1999-08-13 2001-02-22 The Victoria University Of Manchester Phytase enzymes, nucleic acids encoding phytase enzymes and vectors and host cells incorporating same
US7795002B2 (en) * 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
ATE309385T1 (de) * 2000-06-28 2005-11-15 Glycofi Inc Verfahren für die herstellung modifizierter glykoproteine
DK1685244T3 (da) * 2003-11-21 2012-07-02 Danisco Us Inc Ekspression af granulær-stivelse-hydrolyserende enzymer i Trichoderma og fremgangsmåde til fremstilling af glucose fra granulære stivelsessubstrater
WO2007011221A2 (en) * 2005-07-15 2007-01-25 Universiteit Leiden Anti fungal screening method

Also Published As

Publication number Publication date
WO2007123801A1 (en) 2007-11-01
EP2004819B1 (en) 2015-07-22
EP2004819A1 (en) 2008-12-24
US20140377804A1 (en) 2014-12-25
US20110104750A1 (en) 2011-05-05

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