DK185484A - Hybridvektor, der producerer human-parathyroid hormon, samt human-parathyroid hormon-gen - Google Patents

Hybridvektor, der producerer human-parathyroid hormon, samt human-parathyroid hormon-gen Download PDF

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Publication number
DK185484A
DK185484A DK185484A DK185484A DK185484A DK 185484 A DK185484 A DK 185484A DK 185484 A DK185484 A DK 185484A DK 185484 A DK185484 A DK 185484A DK 185484 A DK185484 A DK 185484A
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DK
Denmark
Prior art keywords
human
gene
parathyroid
hybrid vector
hormon
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DK185484A
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English (en)
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DK185484D0 (da
Inventor
Hubert Mayer
Original Assignee
Biotechnolog Forschung Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Biotechnolog Forschung Gmbh filed Critical Biotechnolog Forschung Gmbh
Publication of DK185484D0 publication Critical patent/DK185484D0/da
Publication of DK185484A publication Critical patent/DK185484A/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone (parathormone); Parathyroid hormone-related peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Description

ducerer human-parathyroid hormon (PTH), kan (A) fremstilles ved (a) isolering af mRNA fra porcine parathyroider, (b) kloning af den isolerede mRNA som ds-cDNA ved hjælp af en vektor i E. coli, (c) isolering af hybridvektor-DNA fra pools af resulterende kloner, (d) fiksering af hybridvektor-DNA til en bærer for hver pool, hybridisering af mRNA isoleret ifølge (a) og dernæst fjernelse deraf igen, udførelse af forsøg på at translatere mRNA, der er blevet fjernet, til porcint parathyroid hormon (PTH), der er blevet identificeret ved antistof- -fældning, hvorved der bestemmes kloner, der er opnået ifølge (b), som har porcine parathyroid hormon (PTH)-gensekvenser, sekvensering af hybridvektor-DNA af bestemte kloner med porcine parathyroid hormon(PTH)-gensekvenser, og identifikation af den klon eller de kloner, der har hybridvektor-DNA, med en DNA-sekvens, der koder for porcint parathyroid hormon (PTH), (e) radioaktiv mærkning af hybridvektor-DNA af de ifølge (d) identificerede kloner, (f) screening af en human genbank med resulterende radioaktivt mærket hybridvektor-DNA og (g) isolering af det bestemte human-parathyroid hormon (PTH)-gen (DNA-sekvensen mellem EcoRI-restrik-tionsstederne ifølge (B) og overføring af dette til en ekspressions-hybridvektor, der kan klones i eukaryotiske celler, (B) og denne vektor er karakteriseret ved en DNA-sekvens lokaliseret mellem to EcoRI-restriktions-steder, der inkluderer en specifik DNA-sekvens, eller en subregion af DNA-sekvensen lokaliseret mellem EcoRI-restriktionsstedeme, især en subregion , der udtrykker human-parathyroid hormon (PTH) eller angonister eller antagonister deraf.
Et humant parathyroid hormon (PTH)-gen kan fremstilles ifølge (A) ovenfor og er karakteriseret ved DNA-sekvensen lokaliseret mellem to EcoRI-restriktionssteder ifølge (B) ovenfor eller en subregion deraf.
DK185484A 1983-04-11 1984-04-10 Hybridvektor, der producerer human-parathyroid hormon, samt human-parathyroid hormon-gen DK185484A (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19833312928 DE3312928A1 (de) 1983-04-11 1983-04-11 Human-parathormon produzierende hybridvektoren und human-parathormongen

Publications (2)

Publication Number Publication Date
DK185484D0 DK185484D0 (da) 1984-04-10
DK185484A true DK185484A (da) 1984-10-12

Family

ID=6195950

Family Applications (1)

Application Number Title Priority Date Filing Date
DK185484A DK185484A (da) 1983-04-11 1984-04-10 Hybridvektor, der producerer human-parathyroid hormon, samt human-parathyroid hormon-gen

Country Status (5)

Country Link
EP (1) EP0139076B1 (da)
JP (1) JPS6034986A (da)
AT (1) ATE77834T1 (da)
DE (2) DE3312928A1 (da)
DK (1) DK185484A (da)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4753874A (en) * 1985-07-08 1988-06-28 Board Of Trustees Of The Leland Stanford Junior University Rapid mutation testing system for human cells
EP0383751B1 (en) 1986-10-22 1994-03-09 GAUTVIK, Kaare M., Dr. Production of human parathyroid hormone from microorganisms
US5010010A (en) * 1986-10-22 1991-04-23 Selmer-Sande, A.S. Production of human parathyroid hormone from microorganisms
US5420242A (en) * 1986-10-22 1995-05-30 Kaare M. Gautvik Production of human parathyroid hormone from microorganisms
DE3725320A1 (de) * 1987-07-30 1989-02-09 Biotechnolog Forschung Gmbh Expressionsvektoren und verfahren unter deren verwendung zur gewinnung von cro/ss-galaktosidase/pth-fusionsproteinen und von pth
JPH05271279A (ja) * 1991-08-07 1993-10-19 Takeda Chem Ind Ltd ヒト副甲状腺ホルモンムテインおよびその製造法
EP0528271B1 (en) * 1991-08-07 1997-04-23 Takeda Chemical Industries, Ltd. Human parathyroid hormone muteins and production thereof
SE9702401D0 (sv) 1997-06-19 1997-06-19 Astra Ab Pharmaceutical use
KR100230578B1 (ko) * 1997-07-25 1999-12-01 허영섭 포스포리불로키나제를 융합파트너로 이용하는 재조합 인간 부갑상선호르몬의 발현벡터
CN1301740A (zh) * 1999-12-24 2001-07-04 上海博德基因开发有限公司 一种新的多肽——甲状旁腺激素9和编码这种多肽的多核苷酸

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2963374D1 (en) * 1978-10-10 1982-09-09 Univ Leland Stanford Junior Recombinant dna, method for preparing it and production of foreign proteins by unicellular hosts containing it
ZA811895B (en) * 1980-04-07 1982-04-28 Univ California Expression of hormone genomic clones
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
WO1984001173A1 (en) * 1982-09-15 1984-03-29 Immuno Nuclear Corp Production of mature proteins in transformed yeast

Also Published As

Publication number Publication date
DE3312928C2 (da) 1987-08-27
DE3485797D1 (de) 1992-08-06
DE3312928A1 (de) 1984-11-22
EP0139076A2 (de) 1985-05-02
ATE77834T1 (de) 1992-07-15
EP0139076B1 (de) 1992-07-01
EP0139076A3 (en) 1987-05-20
DK185484D0 (da) 1984-04-10
JPS6034986A (ja) 1985-02-22

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