DK179686A - DNA SECTION OF E. COLI TRP OPERON AND ITS USE - Google Patents
DNA SECTION OF E. COLI TRP OPERON AND ITS USE Download PDFInfo
- Publication number
- DK179686A DK179686A DK179686A DK179686A DK179686A DK 179686 A DK179686 A DK 179686A DK 179686 A DK179686 A DK 179686A DK 179686 A DK179686 A DK 179686A DK 179686 A DK179686 A DK 179686A
- Authority
- DK
- Denmark
- Prior art keywords
- sequence
- dna
- coli
- ligation
- cleavage site
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Electrotherapy Devices (AREA)
- Complex Calculations (AREA)
- Advance Control (AREA)
Abstract
For the contracting states : BE, CH, DE, GB, IT, LI, LU, NL, SE 1. DNA segment of the trp operon from E. coli, characterized by the sequence (coding strand) I 5' CTATCGACC 3' (I) which is connected at the 5' end to the Shine-Dalgarno sequence AAGG, and at the 3' end to the start codon ATG. For the contracting state : AT 1. A process for the preparation of a vector having the trp expression system of E. coli, characterized by cleavage with the restriction enzyme Nco l of an E. coli vector which contains the DNA sequence I 5' GTATCGACC 3' (I) (coding strand) followed by 5' ATGG 3', and a) ligation of a gene structure of DNA sequence II 5' CATG X... 3' 3' Y... 5' (II) in which X and Y denote the first complementary pair of nucleotides downstream of the start codon of a structural gene, in the cleavage site, or b) enzymatic filling in of the cleavage site and ligation of the DNA of the sequence III 5' GTA TCG ACC ATG 3' (III) 3' CAT AGC TGG TAC 5' with a DNA of the sequence IV 5' X... 3' (IV) 3' Y... 5' in which X and Y have the abovementioned meaning, or c) enzymatic degradation of the protruding sequence of the cleavage site, and ligation of the DNA of the sequence VI 5' GTA TCG AC 3' (VI) 3' CAT AGC TG 5' with a DNA of the sequence VII 5' Z ATG X... 3' (VII) 3' Z'TAC Y... 5' Z and Z' denoting any desired pair of nucleotides, which can also be dispensed with.
Description
i hvilken X og Y betyder det første komplementære nucleo-tidpar efter startkodonen af et strukturgen, eller b) opfylder overskæringsstedet enzymatisk og liga-terer ONA'en med sekvensen III: 5’ GTA TCG ACC ATG 3* (III) 3' CAT AGC TGG TAC 5‘ med en DNA med sekvensen IV: 5 * X 3 * 5 X... J (IV) 3’ Y... 5' hvor X og Y har den ovenfor angivne betydning, eller c) enzymatisk nedbryder den udragende sekvens med overskæringsstedet og ligaterer DNA1en med sekvensen VI: 5’ GTA TCG AC 3' (VI) 3’ CAT AGC TG 5' med en DNA med sekvensen VII: 5' Z ATG X... 3’ ivII) 3' Z'TAC Y... 5' hvor Z og Z’ betyder et vilkårligt nucleotidpar, der også kan være fraværende.in which X and Y denote the first complementary nucleotide pair after the start codon of a structural gene, or b) meet the cut-off enzymatically and ligate the ONA with the sequence III: 5 'GTA TCG ACC ATG 3 * (III) 3' CAT AGC TGG TAC 5 'with a DNA of sequence IV: 5 * X 3 * 5 X ... J (IV) 3' Y ... 5 'where X and Y have the meaning given above, or c) enzymatically degrades the protruding sequence with the cut-off site and ligates the DNA1 with sequence VI: 5 'GTA TCG AC 3' (VI) 3 'CAT AGC TG 5' with a DNA of sequence VII: 5 'Z ATG X ... 3' ivII) 3 'Z' TAC Y ... 5 'where Z and Z' mean any nucleotide pair that may also be absent.
Ved en fremgangsmåde til fremstilling af et polypeptid ud fra genetisk kodelige aminosyrer gås der således frem, at man bringer E. coli-celler med et indhold af en som ovenfor fremstillet vektor til ekspression.Thus, a method of producing a polypeptide from genetically encoded amino acids proceeds to express E. coli cells with a content of a vector prepared as above.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3514113 | 1985-04-19 | ||
| DE19853514113 DE3514113A1 (en) | 1985-04-19 | 1985-04-19 | CHANGE OF THE DNA SEQUENCE BETWEEN SHINE-DALGARNO SEQUENCE AND START CODON OF THE TRP OPERON TO INCREASE PROTEIN EXPRESSION |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK179686D0 DK179686D0 (en) | 1986-04-18 |
| DK179686A true DK179686A (en) | 1986-10-20 |
| DK172695B1 DK172695B1 (en) | 1999-05-31 |
Family
ID=6268543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK198601796A DK172695B1 (en) | 1985-04-19 | 1986-04-18 | DNA sections of the trp operon of E. coli, plasmids containing the DNA section, method of producing a vector in |
Country Status (19)
| Country | Link |
|---|---|
| EP (1) | EP0198415B1 (en) |
| JP (1) | JPH0665317B2 (en) |
| KR (1) | KR940004543B1 (en) |
| AT (1) | ATE44046T1 (en) |
| AU (1) | AU600229B2 (en) |
| CA (1) | CA1321963C (en) |
| DE (2) | DE3514113A1 (en) |
| DK (1) | DK172695B1 (en) |
| ES (2) | ES8704541A1 (en) |
| FI (1) | FI84362C (en) |
| GR (1) | GR861022B (en) |
| HU (1) | HU196458B (en) |
| IE (1) | IE58994B1 (en) |
| IL (1) | IL78529A (en) |
| NO (1) | NO175646C (en) |
| NZ (1) | NZ215858A (en) |
| PH (1) | PH26596A (en) |
| PT (1) | PT82417B (en) |
| ZA (1) | ZA862925B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4966849A (en) * | 1985-09-20 | 1990-10-30 | President And Fellows Of Harvard College | CDNA and genes for human angiogenin (angiogenesis factor) and methods of expression |
| AU8379991A (en) * | 1990-09-14 | 1992-03-26 | Astra Aktiebolag | A novel method of generating clones for the expression of unfused proteins |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3247922A1 (en) * | 1982-12-24 | 1984-06-28 | Boehringer Ingelheim International GmbH, 6507 Ingelheim | DNA SEQUENCES, THEIR PRODUCTION, PLASMIDES CONTAINING THESE SEQUENCES AND THE USE THEREOF FOR THE SYNTHESIS OF EUKARYOTIC GENE PRODUCTS IN PROKARYOTS |
| JPS60188077A (en) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | Novel manifestation plasmid having whole sequence of calf prochymosin cdna |
| DE3430683A1 (en) * | 1984-08-21 | 1986-03-06 | Hoechst Ag, 6230 Frankfurt | SYNTHETIC REGULATION REGION |
-
1985
- 1985-04-19 DE DE19853514113 patent/DE3514113A1/en not_active Withdrawn
-
1986
- 1986-04-09 EP EP86104879A patent/EP0198415B1/en not_active Expired
- 1986-04-09 DE DE8686104879T patent/DE3663956D1/en not_active Expired
- 1986-04-09 AT AT86104879T patent/ATE44046T1/en not_active IP Right Cessation
- 1986-04-17 PT PT82417A patent/PT82417B/en unknown
- 1986-04-17 IL IL78529A patent/IL78529A/en not_active IP Right Cessation
- 1986-04-17 PH PH33672A patent/PH26596A/en unknown
- 1986-04-17 ES ES554097A patent/ES8704541A1/en not_active Expired
- 1986-04-17 HU HU861613A patent/HU196458B/en unknown
- 1986-04-17 NZ NZ215858A patent/NZ215858A/en unknown
- 1986-04-17 GR GR861022A patent/GR861022B/en unknown
- 1986-04-17 FI FI861624A patent/FI84362C/en not_active IP Right Cessation
- 1986-04-18 CA CA000507096A patent/CA1321963C/en not_active Expired - Lifetime
- 1986-04-18 DK DK198601796A patent/DK172695B1/en not_active IP Right Cessation
- 1986-04-18 AU AU56387/86A patent/AU600229B2/en not_active Expired
- 1986-04-18 NO NO861551A patent/NO175646C/en unknown
- 1986-04-18 JP JP61089832A patent/JPH0665317B2/en not_active Expired - Lifetime
- 1986-04-18 IE IE103186A patent/IE58994B1/en not_active IP Right Cessation
- 1986-04-18 ZA ZA862925A patent/ZA862925B/en unknown
- 1986-04-19 KR KR1019860003040A patent/KR940004543B1/en not_active IP Right Cessation
- 1986-06-19 ES ES556233A patent/ES8706818A1/en not_active Expired
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B1 | Patent granted (law 1993) | ||
| PUP | Patent expired |