DK0411501T3 - Optimized Fermentation Methods for Preparation of Foreign Proteins in E. coli - Google Patents
Optimized Fermentation Methods for Preparation of Foreign Proteins in E. coliInfo
- Publication number
- DK0411501T3 DK0411501T3 DK90114473.3T DK90114473T DK0411501T3 DK 0411501 T3 DK0411501 T3 DK 0411501T3 DK 90114473 T DK90114473 T DK 90114473T DK 0411501 T3 DK0411501 T3 DK 0411501T3
- Authority
- DK
- Denmark
- Prior art keywords
- lactose
- limitation
- coli
- foreign proteins
- preparation
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4721—Lipocortins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention describes optimised fermentation processes for the production of foreign proteins in E.coli using the lac promoter or improved lac promoter (e.g. tac, trc). After the initial growth phase with glucose as carbon source, product formation is induced (1) by IPTG with glucose limitation or (2) by lactose with lactose limitation or (3) by IPTG and lactose with lactose limitation. The limitation of glucose or lactose is such that the partial pressure of oxygen remains above 10%.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3925550A DE3925550A1 (en) | 1989-08-02 | 1989-08-02 | OPTIMIZED FERMENTATION PROCEDURES FOR THE MANUFACTURE OF FOREIGN PROTEINS IN E.COLI |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0411501T3 true DK0411501T3 (en) | 1995-01-16 |
Family
ID=6386347
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK90114473.3T DK0411501T3 (en) | 1989-08-02 | 1990-07-27 | Optimized Fermentation Methods for Preparation of Foreign Proteins in E. coli |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0411501B1 (en) |
JP (1) | JP2977241B2 (en) |
KR (3) | KR100190777B1 (en) |
AT (1) | ATE111528T1 (en) |
AU (1) | AU624982B2 (en) |
CA (1) | CA2022487C (en) |
DE (2) | DE3925550A1 (en) |
DK (1) | DK0411501T3 (en) |
ES (1) | ES2060876T3 (en) |
IE (1) | IE65040B1 (en) |
PT (1) | PT94876B (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9215550D0 (en) * | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
AT397812B (en) * | 1992-12-18 | 1994-07-25 | Polymun Scient Gmbh | Process for the expression of genes under the control of the lac operator |
KR0151534B1 (en) * | 1995-10-30 | 1998-08-17 | 이종호 | Escherichiacoli for suppressing the production of organic acid |
CN101365785A (en) | 2002-07-01 | 2009-02-11 | 阿基昂生命科学公司,以生物技术资源部的名义经营 | Process and materials for production of glucosamine and n-acetylglucosamine |
EP2460882B1 (en) * | 2009-07-28 | 2017-11-29 | Mitsui Chemicals, Inc. | Method for producing d-lactic acid |
WO2015178466A1 (en) * | 2014-05-21 | 2015-11-26 | 味の素株式会社 | Fibroin-like protein production method |
CN111850072B (en) * | 2019-04-29 | 2022-08-16 | 暨南大学 | Peptibody multi-epitope vaccine fermentation production process and application |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3379506D1 (en) * | 1983-08-22 | 1989-05-03 | Kyowa Hakko Kogyo Kk | Process for producing peptide |
CA1319630C (en) * | 1987-02-19 | 1993-06-29 | Satori Sone | Method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign gene product thereby |
-
1989
- 1989-08-02 DE DE3925550A patent/DE3925550A1/en active Granted
-
1990
- 1990-07-27 DE DE59007117T patent/DE59007117D1/en not_active Expired - Lifetime
- 1990-07-27 ES ES90114473T patent/ES2060876T3/en not_active Expired - Lifetime
- 1990-07-27 EP EP90114473A patent/EP0411501B1/en not_active Expired - Lifetime
- 1990-07-27 DK DK90114473.3T patent/DK0411501T3/en active
- 1990-07-27 AT AT90114473T patent/ATE111528T1/en not_active IP Right Cessation
- 1990-07-31 KR KR1019900011693A patent/KR100190777B1/en not_active IP Right Cessation
- 1990-08-01 AU AU60005/90A patent/AU624982B2/en not_active Expired
- 1990-08-01 IE IE278590A patent/IE65040B1/en not_active IP Right Cessation
- 1990-08-01 CA CA002022487A patent/CA2022487C/en not_active Expired - Lifetime
- 1990-08-01 JP JP2202616A patent/JP2977241B2/en not_active Expired - Lifetime
- 1990-08-01 PT PT94876A patent/PT94876B/en not_active IP Right Cessation
-
1998
- 1998-09-19 KR KR1019980038815A patent/KR100190779B1/en not_active IP Right Cessation
- 1998-09-19 KR KR1019980038814A patent/KR100190778B1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
PT94876A (en) | 1991-04-18 |
ATE111528T1 (en) | 1994-09-15 |
KR100190779B1 (en) | 1999-06-01 |
ES2060876T3 (en) | 1994-12-01 |
JP2977241B2 (en) | 1999-11-15 |
DE3925550A1 (en) | 1991-02-07 |
EP0411501B1 (en) | 1994-09-14 |
DE3925550C2 (en) | 1991-08-08 |
JPH0376595A (en) | 1991-04-02 |
PT94876B (en) | 1997-04-30 |
IE902785A1 (en) | 1991-02-27 |
DE59007117D1 (en) | 1994-10-20 |
KR910004811A (en) | 1991-03-29 |
KR100190777B1 (en) | 1999-06-01 |
CA2022487A1 (en) | 1991-02-03 |
KR100190778B1 (en) | 1999-06-01 |
AU624982B2 (en) | 1992-06-25 |
EP0411501A1 (en) | 1991-02-06 |
IE65040B1 (en) | 1995-10-04 |
AU6000590A (en) | 1991-02-07 |
CA2022487C (en) | 2001-12-11 |
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