AU624982B2 - Optimized fermentation processes for the preparation of foreign proteins in E. Coli - Google Patents

Optimized fermentation processes for the preparation of foreign proteins in E. Coli

Info

Publication number
AU624982B2
AU624982B2 AU60005/90A AU6000590A AU624982B2 AU 624982 B2 AU624982 B2 AU 624982B2 AU 60005/90 A AU60005/90 A AU 60005/90A AU 6000590 A AU6000590 A AU 6000590A AU 624982 B2 AU624982 B2 AU 624982B2
Authority
AU
Australia
Prior art keywords
lactose
limitation
coli
fermentation processes
foreign proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU60005/90A
Other versions
AU6000590A (en
Inventor
Mathias Grote
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Publication of AU6000590A publication Critical patent/AU6000590A/en
Application granted granted Critical
Publication of AU624982B2 publication Critical patent/AU624982B2/en
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention describes optimised fermentation processes for the production of foreign proteins in E.coli using the lac promoter or improved lac promoter (e.g. tac, trc). After the initial growth phase with glucose as carbon source, product formation is induced (1) by IPTG with glucose limitation or (2) by lactose with lactose limitation or (3) by IPTG and lactose with lactose limitation. The limitation of glucose or lactose is such that the partial pressure of oxygen remains above 10%.
AU60005/90A 1989-08-02 1990-08-01 Optimized fermentation processes for the preparation of foreign proteins in E. Coli Expired AU624982B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3925550A DE3925550A1 (en) 1989-08-02 1989-08-02 OPTIMIZED FERMENTATION PROCEDURES FOR THE MANUFACTURE OF FOREIGN PROTEINS IN E.COLI
DE3925550 1989-08-02

Publications (2)

Publication Number Publication Date
AU6000590A AU6000590A (en) 1991-02-07
AU624982B2 true AU624982B2 (en) 1992-06-25

Family

ID=6386347

Family Applications (1)

Application Number Title Priority Date Filing Date
AU60005/90A Expired AU624982B2 (en) 1989-08-02 1990-08-01 Optimized fermentation processes for the preparation of foreign proteins in E. Coli

Country Status (11)

Country Link
EP (1) EP0411501B1 (en)
JP (1) JP2977241B2 (en)
KR (3) KR100190777B1 (en)
AT (1) ATE111528T1 (en)
AU (1) AU624982B2 (en)
CA (1) CA2022487C (en)
DE (2) DE3925550A1 (en)
DK (1) DK0411501T3 (en)
ES (1) ES2060876T3 (en)
IE (1) IE65040B1 (en)
PT (1) PT94876B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215550D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
AT397812B (en) * 1992-12-18 1994-07-25 Polymun Scient Gmbh Process for the expression of genes under the control of the lac operator
KR0151534B1 (en) * 1995-10-30 1998-08-17 이종호 Escherichiacoli for suppressing the production of organic acid
MXPA04012978A (en) 2002-07-01 2005-09-12 Arkion Life Sciences Llc D B A Process and materials for production of glucosamine and n-acetylglucosamine.
BR112012001917B1 (en) * 2009-07-28 2018-10-16 Mitsui Chemicals Inc method to produce lactic acid
WO2015178466A1 (en) 2014-05-21 2015-11-26 味の素株式会社 Fibroin-like protein production method
CN111850072B (en) * 2019-04-29 2022-08-16 暨南大学 Peptibody multi-epitope vaccine fermentation production process and application

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3379506D1 (en) * 1983-08-22 1989-05-03 Kyowa Hakko Kogyo Kk Process for producing peptide
CA1319630C (en) * 1987-02-19 1993-06-29 Satori Sone Method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign gene product thereby

Also Published As

Publication number Publication date
ES2060876T3 (en) 1994-12-01
DK0411501T3 (en) 1995-01-16
EP0411501B1 (en) 1994-09-14
DE3925550C2 (en) 1991-08-08
DE3925550A1 (en) 1991-02-07
KR910004811A (en) 1991-03-29
IE902785A1 (en) 1991-02-27
AU6000590A (en) 1991-02-07
KR100190778B1 (en) 1999-06-01
DE59007117D1 (en) 1994-10-20
JP2977241B2 (en) 1999-11-15
CA2022487C (en) 2001-12-11
ATE111528T1 (en) 1994-09-15
PT94876B (en) 1997-04-30
KR100190779B1 (en) 1999-06-01
JPH0376595A (en) 1991-04-02
EP0411501A1 (en) 1991-02-06
IE65040B1 (en) 1995-10-04
KR100190777B1 (en) 1999-06-01
PT94876A (en) 1991-04-18
CA2022487A1 (en) 1991-02-03

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