CA2022487A1 - Optimized fermentation processes for the preparation of foreign proteins in e.coli - Google Patents

Optimized fermentation processes for the preparation of foreign proteins in e.coli

Info

Publication number
CA2022487A1
CA2022487A1 CA002022487A CA2022487A CA2022487A1 CA 2022487 A1 CA2022487 A1 CA 2022487A1 CA 002022487 A CA002022487 A CA 002022487A CA 2022487 A CA2022487 A CA 2022487A CA 2022487 A1 CA2022487 A1 CA 2022487A1
Authority
CA
Canada
Prior art keywords
lactose
limitation
coli
preparation
fermentation processes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002022487A
Other languages
French (fr)
Other versions
CA2022487C (en
Inventor
Mathias Grote
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Publication of CA2022487A1 publication Critical patent/CA2022487A1/en
Application granted granted Critical
Publication of CA2022487C publication Critical patent/CA2022487C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention describes optimized fermentation processes for the preparation of foreign proteins in E.coli using the lac promoter or improved lac promoter (for example tac, trc). After the initial growth phase with glucose as carbon source, induction of product formation is effected (1) via IPTG with glucose limitation or (2) via lactose with lactose limitation or (3) via IPTG and lactose with lactose limitation. The limitation of glucose or lactose is such that the oxygen partial pressure remains above 10%.
CA002022487A 1989-08-02 1990-08-01 Optimized fermentation processes for the preparation of foreign proteins in e.coli Expired - Lifetime CA2022487C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3925550A DE3925550A1 (en) 1989-08-02 1989-08-02 OPTIMIZED FERMENTATION PROCEDURES FOR THE MANUFACTURE OF FOREIGN PROTEINS IN E.COLI
DEP3925550.6 1989-08-02

Publications (2)

Publication Number Publication Date
CA2022487A1 true CA2022487A1 (en) 1991-02-03
CA2022487C CA2022487C (en) 2001-12-11

Family

ID=6386347

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002022487A Expired - Lifetime CA2022487C (en) 1989-08-02 1990-08-01 Optimized fermentation processes for the preparation of foreign proteins in e.coli

Country Status (11)

Country Link
EP (1) EP0411501B1 (en)
JP (1) JP2977241B2 (en)
KR (3) KR100190777B1 (en)
AT (1) ATE111528T1 (en)
AU (1) AU624982B2 (en)
CA (1) CA2022487C (en)
DE (2) DE3925550A1 (en)
DK (1) DK0411501T3 (en)
ES (1) ES2060876T3 (en)
IE (1) IE65040B1 (en)
PT (1) PT94876B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215550D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
AT397812B (en) * 1992-12-18 1994-07-25 Polymun Scient Gmbh Process for the expression of genes under the control of the lac operator
KR0151534B1 (en) * 1995-10-30 1998-08-17 이종호 Escherichiacoli for suppressing the production of organic acid
JP2006508643A (en) * 2002-07-01 2006-03-16 アーキオン ライフ サイエンシーズ エルエルシー ディー／ビー／エー バイオ−テクニカル リソーセズ ディビジョン Processes and materials for the production of glucosamine and N-acetylglucosamine
BR112012001917B1 (en) * 2009-07-28 2018-10-16 Mitsui Chemicals Inc method to produce lactic acid
JPWO2015178466A1 (en) * 2014-05-21 2017-04-20 味の素株式会社 Production method of fibroin-like protein
CN111850072B (en) * 2019-04-29 2022-08-16 暨南大学 Peptibody multi-epitope vaccine fermentation production process and application

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3379506D1 (en) * 1983-08-22 1989-05-03 Kyowa Hakko Kogyo Kk Process for producing peptide
CA1319630C (en) * 1987-02-19 1993-06-29 Satori Sone Method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign gene product thereby

Also Published As

Publication number Publication date
ATE111528T1 (en) 1994-09-15
IE65040B1 (en) 1995-10-04
IE902785A1 (en) 1991-02-27
JP2977241B2 (en) 1999-11-15
DE3925550C2 (en) 1991-08-08
KR100190777B1 (en) 1999-06-01
ES2060876T3 (en) 1994-12-01
KR100190779B1 (en) 1999-06-01
KR100190778B1 (en) 1999-06-01
AU6000590A (en) 1991-02-07
DE3925550A1 (en) 1991-02-07
AU624982B2 (en) 1992-06-25
EP0411501B1 (en) 1994-09-14
PT94876B (en) 1997-04-30
CA2022487C (en) 2001-12-11
JPH0376595A (en) 1991-04-02
KR910004811A (en) 1991-03-29
DK0411501T3 (en) 1995-01-16
PT94876A (en) 1991-04-18
EP0411501A1 (en) 1991-02-06
DE59007117D1 (en) 1994-10-20

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