DE947192C - Process for the production of a healing serum for the therapy of rheumatism, in particular of primary chronic polyarthritis - Google Patents

Description

Process for the production of a healing serum for the therapy of rheumatism, especially primary chronic polyarthritis It is known that polyarthritis usually experiences a considerable improvement through a pregnancy, even a short-term one Symptoms may disappear.

On the basis of clinical investigations it was found that in the serum or the erythrocytes of the retroplacental blood a specifically effective substance against the Polyarthritis is present, which, as clinically and experimentally determined, among other things as an activator of the adrenal cortex in the sense of an immunobiological favorable »stress« is effective.

The invention relates to the production of a medicinal serum using it this active ingredient, which is found in the serum or the erythrocytes of the red placenta blood is available. The invention is that with increasing amounts of a higher aliphatic alcohol extracted, if necessary under the action of ultrasound, Lipoid active ingredients dried in a vacuum from the retroplacental blood with the to dryness brought ether-butanol extract from the remaining erythrocyte recovery Serum converted into a water-soluble form with the help of sodium deoxycholate-glycine will.

Here, the lipoid active ingredients are separated by the previously Immune globulin portion of the serum complements again because only the Lipoid active ingredients with the immune globulin component are effective. The binding of the lipoid active ingredients no the immune globulin portion is here during the dissolution of the lipoids by the Treatment with sodium deoxycholate-glycine achieved. This effect arises as a result a loose bond in the sense of a chemical reaction that only occurs through hydrolytic Cleavage in the organism becomes free again: Both through the dissolving process take place a solution as well as a binding of clinically particularly valuable and active ingredients, while dietary fiber or clinically inactive substances are not recorded.

In one embodiment of the invention, before the separation of the immune globulin (gamma globulin) the serum of a treatment with ultrasound be subjected. This sonication ensures that the molecular surface certain types of protein initially reduced in size, but then when the sonication is continued is enlarged.

Example The resulting from 500 ccm of freshly obtained retroplacental blood Erythrocyte pulp becomes larger with increasing amounts over the course of 48 hours aliphatic alcohol, e.g. B. n-butanol, extracted under the action of ultrasound. The solvent is then separated off in vacuo and the resulting lipoid body dried under vacuum.

The serum remaining after the erythrocyte collection is extracted first with ether, then with increasing amounts of butanol over a period of 30 hours with frequent shaking. After separation in a separating funnel, the solvent mixture is distilled off in vacuo, dried and combined with the lipoid body from the erythrocytes. The combined lipoid complex is dissolved in a mixture of sodium deoxycholate-glycine by prolonged shaking.

The serum obtained in the above lipoid extraction becomes short sonicated and the immune globulin (gamma globulin) according to known methods separated, purified by electrodialysis and in an isotonic solution of Glycine-phosphate buffer mixture dissolved according to S o e r e n s e n p $ 6.8. This solution is combined with the lipoid complex and then passed through EKS i filter sheets Filtered sterile and pyrogen-free.

Claims (1)

PATENT CLAIMS: i. Process for the production of a preparation for therapeutic purposes which is suitable for combination purposes and contains the lipoid active substances from the erythrocytes and the serum of the retroplacental blood, characterized in that the lipoid active substances extracted in a vacuum from the retroplacental blood with increasing amounts of a higher aliphatic alcohol, optionally under the action of ultrasound the ether-butanol extract brought to dryness from the serum remaining after the erythrocyte recovery can be brought into a water-soluble form with the aid of sodium deoxycholate-glycine. z. Process according to Claim i, characterized in that the serum is subjected to an ultrasound treatment even before the immunoglobulin (gamma globulin) is separated off.