DE875856C - Process for the simultaneous extraction of the blood sugar lowering hormone and the ferments from the pancreas - Google Patents

Process for the simultaneous extraction of the blood sugar lowering hormone and the ferments from the pancreas

Info

Publication number
DE875856C
DE875856C DEF3578D DEF0003578D DE875856C DE 875856 C DE875856 C DE 875856C DE F3578 D DEF3578 D DE F3578D DE F0003578 D DEF0003578 D DE F0003578D DE 875856 C DE875856 C DE 875856C
Authority
DE
Germany
Prior art keywords
pancreas
ferments
blood sugar
sugar lowering
hormone
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEF3578D
Other languages
German (de)
Inventor
Fritz Dr Lindner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Priority to DEF3578D priority Critical patent/DE875856C/en
Application granted granted Critical
Publication of DE875856C publication Critical patent/DE875856C/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C07K14/625—Extraction from natural sources

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Description

Verfahren zur gleichzeitigen Gewinnung des blutzuckersenkenden Hormons und der Fermente aus Bauchspeicheldrüsen Bei .der Gewinnung .des Inselhormons aus Bauchspeicheldrüsen wird zur Extraktion ausschließlich mit Mineralsäuren angesäuerter wässeriger Alkohol verwendet. Dabei werden die Fermente der Bauchspeicheldrüse in kurzer Zeit vollständig vernichtet.Process for the simultaneous production of the blood sugar lowering hormone and the ferments from pancreas in .the extraction of .the islet hormone from For extraction, the pancreas is acidified exclusively with mineral acids aqueous alcohol used. This is where the pancreas' ferments are in completely destroyed in a short time.

Es wurde nun gefunden, daß man das Hormon unter weitgehender Schonung ,der Fermente extrahieren kann, wenn man zum Ansäuern schwächere anorganische Säuren, wie z. B. Phosphorsäure oder phosphorige Säure, oder organische Säuren, insbesondere Säuren der aliphatischen Reihe, verwendet.It has now been found that the hormone can be used with great care which can extract ferments if weaker inorganic acids are used for acidification, such as B. phosphoric acid or phosphorous acid, or organic acids, in particular Acids of the aliphatic series are used.

Man hat zwar schon früher vereinzelt mit solchen Säuren gearbeitet, die Ausbeuten waren aber unter den dabei angewandten Bedingungen so schlecht, daß diese Verfahren nicht weiter verfolgt wurden (vgl. B om s k o w, Methoden der Hormonforschung, Bd. i, S. 669). Die Gewinnung von Fermenten aus den Extraktionsrückständen ist - nicht versucht worden.It is true that one has worked with such acids in isolated cases in the past, but the yields were so bad under the conditions used that these procedures were not pursued further (see B om s k o w, Methods of Hormone Research, Vol. I, p. 669). The extraction of ferments from the extraction residues is - not tried.

Beispiel z lcg eingefrorene Schweinepankreas werden fein gemah 1en und mit 5,2 1 Aceton und goo ccm Wasser unter Eiskühlung i Stunde kräftig verrührt, wobei mit Ameisensäure auf schwach kongosaure Reaktion eingestellt wird. Nach .dem Abschleudern wird nochmals mit 61 7o°/oigem Aceton unter denselben Bedingungen a Stunden extrahiert. Die Extrakte werden nach bekannten Verfahren auf Inselhormon verarbeitet, man erhält etwa aooo Einheiten pro Kilogramm an reinem Hormon.Example z lcg frozen porcine pancreas are finely ground and stirred vigorously with 5.2 l acetone and goo ccm water while cooling with ice for 1 hour, with formic acid being used to adjust to a weakly Congo-acidic reaction. After this It is spun off again with 61 70% acetone under the same conditions a hours extracted. The extracts are based on islet hormone according to known methods processed, you get about aooo units per kilogram of pure hormone.

Die Rückstände werden sofort .unter Neutralisieren mit Ammoniak mit Aceton unid Äther getrocknet. Man erhält ein Trockenpulver, das etwa die Hälfte des. in der frischen Drüse vorhandenen Fermentgehaltes zeigt.The residues are immediately. Neutralizing with ammonia with Dried acetone unid ether. You get a dry powder that is about half shows the ferment content present in the fresh gland.

An Stelle von Aceton können beispielsweise auch niedere aliphatische Alkohole verwendet werden.Instead of acetone, for example, lower aliphatic Alcohols are used.

Claims (1)

PATENTANSPRUCH: Verfahren zur gleichzeitigen Gewinnung des blutzuckersenkenden Hormons und .der Fermente aus Bauchspeicheldrüsen, dadurch gekennzeichnet, daß man .die Drüsen mit wässerigorganischen Lösungsmitteln in Gegenwart von schwächeren anorganischen oder von organischen Säuren extrahiert, aus :dem Extrakt das Hormon gewinnt .und den Rückstand unter Neutralisieren in an sich bekannter Weise auf Fermentpräparate verarbeitet.PATENT CLAIM: Process for the simultaneous production of the blood sugar lowering agent Hormones and .der enzymes from pancreas, characterized in that one .the glands with aqueous organic solvents in the presence of weaker ones inorganic or extracted from organic acids, from: the extract the hormone wins .und the residue while neutralizing in a known manner on fermentation preparations processed.
DEF3578D 1942-11-21 1942-11-21 Process for the simultaneous extraction of the blood sugar lowering hormone and the ferments from the pancreas Expired DE875856C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEF3578D DE875856C (en) 1942-11-21 1942-11-21 Process for the simultaneous extraction of the blood sugar lowering hormone and the ferments from the pancreas

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEF3578D DE875856C (en) 1942-11-21 1942-11-21 Process for the simultaneous extraction of the blood sugar lowering hormone and the ferments from the pancreas

Publications (1)

Publication Number Publication Date
DE875856C true DE875856C (en) 1953-05-07

Family

ID=7083831

Family Applications (1)

Application Number Title Priority Date Filing Date
DEF3578D Expired DE875856C (en) 1942-11-21 1942-11-21 Process for the simultaneous extraction of the blood sugar lowering hormone and the ferments from the pancreas

Country Status (1)

Country Link
DE (1) DE875856C (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1036469B (en) * 1956-02-23 1958-08-14 Organon Nv Process for the production of protaminase-free insulin preparations
DE975095C (en) * 1955-01-01 1961-08-10 Roehm & Haas G M B H Method for pickling animal bare with pancreatic enzyme preparations

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE975095C (en) * 1955-01-01 1961-08-10 Roehm & Haas G M B H Method for pickling animal bare with pancreatic enzyme preparations
DE1036469B (en) * 1956-02-23 1958-08-14 Organon Nv Process for the production of protaminase-free insulin preparations

Similar Documents

Publication Publication Date Title
DE875856C (en) Process for the simultaneous extraction of the blood sugar lowering hormone and the ferments from the pancreas
DE2460334C3 (en) Process for obtaining insulin and pancreatin from porcine pancreas
DE874062C (en) Process for the simultaneous extraction of the islet hormone and the ferments from the pancreas
DE619977C (en) Process for the production of citric acid by fermentation
DE629416C (en) Process for the production of herbal amylase preparations
DE637258C (en) Process for the production of ascorbic acid
DE744417C (en) Process for producing a digitally-like-looking substance from the thymus
DE954284C (en) Process for obtaining a kallikrein inactivator
DE911134C (en) Process for obtaining caffeine from dried kola nut
DE824781C (en) Process for the production of diastatic malt flours
DE937246C (en) Process for obtaining the active ingredients of Radix Liquiritiae
DE745284C (en) Process for the simultaneous production of ferment preparations in addition to the blood sugar lowering hormone from pancreas
DE973249C (en) Process for the production of injectable solutions from fresh placenta
AT112425B (en) Process for the preparation of highly effective extracts from gonads.
DE874063C (en) Process for the simultaneous production of ferment and hormone preparations
DE856149C (en) Process for the preparation of compounds of sex hormones
DE719026C (en) Process for the production of preparations containing the circulatory hormone kallikrein
AT159196B (en) Process for obtaining the lactation-promoting substance from the anterior pituitary gland.
AT217636B (en) Process for the production of a readily water-soluble protein preparation for the diagnosis of carcinoma
DE824207C (en) Process for the preparation of a sulfur dioxide compound of p-amino-diaethylaniline
DE741472C (en) Method of separating pituitary hormones
DE734244C (en) Process for the superficial saponification of fibers made from acetyl cellulose
DE545973C (en) Process for the preparation of highly effective extracts from female internal secretory organs
DE627272C (en) Process for obtaining the female sex hormone
DE701562C (en) Method for purifying gonad hormones