DE753010C - Pectolytic enzyme preparations - Google Patents

Pectolytic enzyme preparations

Info

Publication number
DE753010C
DE753010C DEI72552D DEI0072552D DE753010C DE 753010 C DE753010 C DE 753010C DE I72552 D DEI72552 D DE I72552D DE I0072552 D DEI0072552 D DE I0072552D DE 753010 C DE753010 C DE 753010C
Authority
DE
Germany
Prior art keywords
enzyme
pectolytic
kieselguhr
enzyme preparations
pectolytic enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEI72552D
Other languages
German (de)
Inventor
Arnold Dr Bohne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IG Farbenindustrie AG
Original Assignee
IG Farbenindustrie AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IG Farbenindustrie AG filed Critical IG Farbenindustrie AG
Priority to DEI72552D priority Critical patent/DE753010C/en
Application granted granted Critical
Publication of DE753010C publication Critical patent/DE753010C/en
Expired legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)

Description

Pektolytisch wirkende Enzympräparate Es ist bekannt, daß Pektin durch pektolvtisch wirkende Enzyme abgebaut wird. Solche Präparate, die gegebenenfalls noch protcolytische und diastatische Enzyme enthalten, sind im Handel. Zur Klärung von Fruchtsäften hat man vorgeschlagen, solche 1?tlzyttthr.iharate zusammen mit Kieselsol anzuwenden. Enzymatisch geklärte Fruchtsäfte hat man bereits mit Hilfe von Kieselgur filtriert.Pectolytic enzyme preparations It is known that pectin by pectolvic enzymes are broken down. Such preparations, if necessary still contain protcolytic and diastatic enzymes are commercially available. To clarify of fruit juices one has suggested such 1? tlzyttthr.iharate along with Apply silica sol. You can already have enzymatically clarified fruit juices with help filtered by kieselguhr.

Die vorliegende Erfindung betrifft ein pektolytisch wirkendes Präparat auf der Grundlage von pektolytischen Enzymen, dem noch Kieselgur beigemengt ist. Die Erfindung beruht auf der Erkenntnis, daB der Pektinabbau unter der Einwirkung pektolytischer Enzyme erheblich durch den Zusatz von Kieselgur beschleunigt wird. Das Verhältnis zwischen dem pektolytisch wirkenden Enzympräparat und der Kiese.lgur kann hierbei in weiten Grenzen schwanken. Beispielsweise können auf ein Teil eines der handelsüblichen pektolytisch wirkenden Handelstrockenpräparate 1/a bis a Teile Kieselgur angewendet werden. Besonders bewährt hat sich ein Mischungsverhältnis von etwa r : i. Die pektinabbauende Wirkung eines solchen Präparates ist ungefähr der eines reinenEnzympräparates mit der 1,25- bis i,5fachen Menge Enzym gleichzusetzen.The present invention relates to a pectolytic preparation based on pectolytic enzymes to which kieselguhr is added. The invention is based on the knowledge that pectin degradation under the action pectolytic enzymes are considerably accelerated by the addition of kieselguhr. The relationship between the pectolytic enzyme preparation and the Kiese.lgur can vary within wide limits. For example, on part of a of the commercially available pectolytic dry preparations 1 / a to a parts Diatomaceous earth can be applied. A mixing ratio has proven particularly useful from about r: i. The pectin-degrading effect of such a preparation is approximate that of a pure enzyme preparation with 1.25 to 1.5 times the amount To equate enzyme.

Zum Zwecke des Pektinabbaues, beispielsweise in Fruchtsäften, gibt man vorteilhaft die fertige Mischung von Enzympräparat und Kieselgur zu. Man kann hierbei von Enzymtrocken- oder -flüssigpräparaten ausgehen. Man kann jedoch auch die Kieselgur nach dem Enzympräparat zugeben, sofern der durch das Enzympräparat verursachte Pektinabbau noch nicht sehr weit fortgeschritten ist. Beispiel i Ein aus Schimmelpilzkulturen in bekannter Weise gewonnenes Filtrationsenzym, welches vorzugsweise pektolytische Enzyme enthält, wird mit reiner Kieselgur, wie sie in der Getränkeindustrie als Filterhilfsmittel verwandt wird, im Verhältnis i : i gemischt. Bei der Klärung von iooo 1 Apfelsaft erzielt man durch die Anwendung von i5oo g Filtrationsenzym-Kieselgur-Gemisch, enthaltend 7509 Enzym, denselben pektoly tischen Effekt, als wenn man mit iooo g reinem Enzym gearbeitet hätte. Beispiel e Eine verdünnte Apfelpektinlösung (PH = 3,2) zeigt in einem Ostwaldschen Viskosimeter (Wasserwert 41 Sekunden) eine Durchlaufgeschwindigkeit von 226 Sekunden. Von dieser Lösung werden 5oo ccm mit i g Filtrationsenzym und weitere 5oo ccm mit 1,s g Filtrationsenzym-Kieselgur-Gemisch, enthaltend o.75 g Enzym, 2 Stunden bei 30° stehengelassen. Im ersteren Fall wird eine Durchlaufgeschwindigkeit von 82 Sekunden, im letzteren von 8o Sekunden erzielt. Eine Behandlung der Pektinlösung mit Kieselgur allein verändert die Viskosität praktisch nicht.For the purpose of breaking down pectin, for example in fruit juices, it is advantageous to add the finished mixture of enzyme preparation and kieselguhr. You can start from dry or liquid enzyme preparations. However, the kieselguhr can also be added after the enzyme preparation, provided that the pectin degradation caused by the enzyme preparation has not yet progressed very far. Example i A filtration enzyme obtained in a known manner from mold cultures, which preferably contains pectolytic enzymes, is mixed with pure diatomaceous earth, as used in the beverage industry as a filter aid, in the ratio i: i. When clarifying 10000 apple juice, the same pectolytic effect is achieved by using 1500 g filtration enzyme-kieselguhr mixture containing 7509 enzyme as if one had worked with 1000 g pure enzyme. Example e A dilute apple pectin solution (pH = 3.2) shows a flow rate of 226 seconds in an Ostwald viscometer (water value 41 seconds). 500 cc of this solution are left to stand for 2 hours at 30 ° with ig filtration enzyme and a further 500 cc with 1, so-called filtration enzyme-kieselguhr mixture, containing 75 g of enzyme. In the former case, a throughput speed of 82 seconds is achieved, in the latter case of 80 seconds. Treatment of the pectin solution with kieselguhr alone practically does not change the viscosity.

Claims (1)

PATENTANSPRUCH: Pektolytisch wirkende Enzympräparate, gekennzeichnet durch einen Zusatz von Kieselgur. PATENT CLAIM: Pectolytic enzyme preparations, characterized by the addition of kieselguhr.
DEI72552D 1942-06-23 1942-06-23 Pectolytic enzyme preparations Expired DE753010C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEI72552D DE753010C (en) 1942-06-23 1942-06-23 Pectolytic enzyme preparations

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEI72552D DE753010C (en) 1942-06-23 1942-06-23 Pectolytic enzyme preparations

Publications (1)

Publication Number Publication Date
DE753010C true DE753010C (en) 1954-11-15

Family

ID=7197245

Family Applications (1)

Application Number Title Priority Date Filing Date
DEI72552D Expired DE753010C (en) 1942-06-23 1942-06-23 Pectolytic enzyme preparations

Country Status (1)

Country Link
DE (1) DE753010C (en)

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