DE70281C - Process for the preparation of albumosis-free peptones - Google Patents

Description

PATENTAM

IMPERIAL

A technically viable method for the preparation of albumosis-free peptones is up to now not known. The so-called in the trade. Peptones are all mixtures of albumoses and peptones in different ratios. So far one has a method of separating albumose and peptone applied to the precipitability of the. Albumose by sulfuric acid Ammonia based; however, this method is only achieved under the conditions to be specified in the same way its purpose.

That by enzymes, acids, alkalis or overheating with water from albumin substances mixture of albumose and peptones produced by every type and origin by salting out with sulfuric acid ammonia only then completely from the albumose released when saturation happens with salt

ι. in dilute solution,

2. one after the other in neutral, alkaline and acidic solution,

3. at the boiling point with subsequent cooling, with the cleaning progressing the albumoses are excreted and the peptones remain dissolved.

The method is based on the observation that pure peptone solutions do not exist with neutral solutions clouded by saturation with ammonium sulphate, even in the case of an acidic or an alkaline reaction will. On the other hand, the usual albumose solutions, after being salted out in a neutral solution, are also made by means of ammonium sulphate tarnished by the addition of alkalis and also of acids from the same body. Deuteroalbumoses are only precipitated in an acidic solution by salting out.

Example 1:

5 kg washed and out preistes Blutfibrin be with pepsin, obtained by digestion of 750 g of the gastric mucosa in 15 of 1N hydrochloric acid of 0.4 per cent., Held for 14 days at 40 ⁰ C., neutralized with caustic soda, filtered off, weakly acidified with acetic acid, cooked, the further filtered solution is at first somewhat concentrated and saturated with ammonium sulphate at the boiling point. If the cooled solution still gives turbidity when strongly diluted with a saturated solution of ammonium sulphate, it has been treated in an excessively concentrated state with the ammonium salt and must be diluted with the salt solution (that is, salted with larger amounts of this solution) and heated again.

The necessary degree of dilution is thus obtained from the sample every time; the solution must remain clear even with the addition of the greatest excess of ammonium sulphate. the The explanation of the process lies in the fact that the precipitation of many substances by salting out is not the only thing depends on the saturation with salt, but also on the weight ratio of the dissolved Salt to the salting-out substance.

Are the albumoses partly in a neutral or extremely weakly acidic solution If the ammonium sulphate is precipitated, certain quantities of it still remain in solution, which are sometimes only with an alkaline, sometimes with a rather strongly acidic reaction in the presence excrete the ammoh sulfate.

Claims (1)

The necessity, first of all, to let an alkaline reaction with ammonia prevail, seems to be based on the fact that the ammonium sulphate dissociates and becomes acidic on boiling will. The fact is that some albumose is also precipitated here, which is the case with saurer Reaction goes back into solution. Finally, in order to remove the deuteroalbumose, a rather strong acidification with acetic acid is necessary necessary. The peptones are isolated from the solution, which is finally obtained free of albumoses Evaporation, cooling and Auskrystallisiren the ammonium sulfate, further elimination of the Salt with moderate amounts of alcohol, boiling with carbonate of barite with added of ammonia and carbonate of ammonia, whereby the barium peptonate from Barium can be freed. Ammonia retained by the peptone can be easily removed by neutralizing with hydrochloric acid and removing the chlorammonium with not to concentrated alcohol. The peptone is produced by repeated precipitations with alcohol with the addition of very little Sodium chloride or other soluble salts, without which no filterable or settling salts are possible Precipitation occurs, further cleaned. * Example 2: 5 kg of fibrin are combined with 5 l of weakly alkaline trypsin solution obtained from 1.5 kg fresh ox pancreas, kept for 8 days at 40 ° C., with the addition of 1 pCt. Thymol to prevent putrefaction, weakly acidified with acetic acid, boiled, filtered and the Solution treated as in example 1. Commercial peptone can take the place of fibrin; in this case, however, are at treatment with pepsin and hydrochloric acid each 40 to 50 g of hydrogen chloride per kilogram to add more of the commercial peptone. Patent claim: Process for the preparation of albumose-free peptones, consisting in that one for the separation the albumose from the mix of albumose with pep tones the saturation with sulfuric acid ammonia in dilute solution, one after the other in neutral, alkaline and acidic solution and at the boiling point with subsequent cooling.