DE69912443D1 - Oligomerreinigung durch selektion mittels zweier enden - Google Patents

Oligomerreinigung durch selektion mittels zweier enden

Info

Publication number
DE69912443D1
DE69912443D1 DE69912443T DE69912443T DE69912443D1 DE 69912443 D1 DE69912443 D1 DE 69912443D1 DE 69912443 T DE69912443 T DE 69912443T DE 69912443 T DE69912443 T DE 69912443T DE 69912443 D1 DE69912443 D1 DE 69912443D1
Authority
DE
Germany
Prior art keywords
oligomer
capture moiety
support
cleaning
selection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69912443T
Other languages
English (en)
Other versions
DE69912443T2 (de
Inventor
Thomas Horn
S Urdea
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Corp filed Critical Bayer Corp
Application granted granted Critical
Publication of DE69912443D1 publication Critical patent/DE69912443D1/de
Publication of DE69912443T2 publication Critical patent/DE69912443T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Heterocyclic Compounds That Contain Two Or More Ring Oxygen Atoms (AREA)
  • Peptides Or Proteins (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
  • Detergent Compositions (AREA)
  • Developing Agents For Electrophotography (AREA)
DE69912443T 1998-08-27 1999-08-27 Oligomerreinigung durch selektion mittels zweier enden Expired - Fee Related DE69912443T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9835798P 1998-08-27 1998-08-27
US98357P 1998-08-27
PCT/US1999/019826 WO2000012524A1 (en) 1998-08-27 1999-08-27 Purification of oligomers using dual-end selection

Publications (2)

Publication Number Publication Date
DE69912443D1 true DE69912443D1 (de) 2003-12-04
DE69912443T2 DE69912443T2 (de) 2004-08-19

Family

ID=22268925

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69912443T Expired - Fee Related DE69912443T2 (de) 1998-08-27 1999-08-27 Oligomerreinigung durch selektion mittels zweier enden

Country Status (8)

Country Link
US (1) US6472522B1 (de)
EP (1) EP1105404B1 (de)
JP (1) JP2002525288A (de)
AT (1) ATE253075T1 (de)
AU (1) AU6023099A (de)
DE (1) DE69912443T2 (de)
ES (1) ES2211222T3 (de)
WO (1) WO2000012524A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69909972T2 (de) 1998-02-11 2004-05-13 University Of Houston, Houston Vorrichtung zur durchführung chemischer und biochemischer reaktionen unter anwendung von photoerzeugten reagenzien
US7094351B2 (en) * 2000-10-12 2006-08-22 Corcoran Robert C Purification of substances by reaction affinity chromatography
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
US20040161749A1 (en) * 2003-01-24 2004-08-19 Hall Jeff G. 3' end tagged oligonucleotides
EP1771579A4 (de) * 2004-05-04 2011-04-20 Dna Twopointo Inc Design, synthese und anordnung von synthetischen nukleinsäuren
EP1778867B1 (de) 2004-07-01 2010-04-28 Gen-Probe Incorporated Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe
US20060286572A1 (en) * 2005-05-16 2006-12-21 Kohne David E Method for producing chemically synthesized and in vitro enzymatically synthesized nucleic acid oligomers
JP4984990B2 (ja) * 2006-03-28 2012-07-25 富士通株式会社 機能性分子の製造方法
CA2723726C (en) 2008-05-13 2017-09-12 Michael M. Becker Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
JP2012509675A (ja) * 2008-11-25 2012-04-26 ジェン−プロウブ インコーポレイテッド 低分子ｒｎａを検出するための組成物および方法、ならびにその使用
US8158391B2 (en) 2009-05-06 2012-04-17 Dna Twopointo, Inc. Production of an α-carboxyl-ω-hydroxy fatty acid using a genetically modified Candida strain
CN107961768A (zh) * 2017-12-06 2018-04-27 苏州博进生物技术有限公司 以吲哚-3-乙酰肼为功能配基的层析介质

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5430136A (en) * 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
US5994069A (en) * 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages

Also Published As

Publication number Publication date
ATE253075T1 (de) 2003-11-15
WO2000012524A1 (en) 2000-03-09
EP1105404A1 (de) 2001-06-13
EP1105404B1 (de) 2003-10-29
AU6023099A (en) 2000-03-21
US6472522B1 (en) 2002-10-29
DE69912443T2 (de) 2004-08-19
ES2211222T3 (es) 2004-07-01
JP2002525288A (ja) 2002-08-13

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Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee