DE69720026T2 - METHOD FOR PRODUCING D-AMINO ACIDS BY DIRECT FERMENTATION - Google Patents

METHOD FOR PRODUCING D-AMINO ACIDS BY DIRECT FERMENTATION Download PDF

Info

Publication number
DE69720026T2
DE69720026T2 DE69720026T DE69720026T DE69720026T2 DE 69720026 T2 DE69720026 T2 DE 69720026T2 DE 69720026 T DE69720026 T DE 69720026T DE 69720026 T DE69720026 T DE 69720026T DE 69720026 T2 DE69720026 T2 DE 69720026T2
Authority
DE
Germany
Prior art keywords
cell
amino acids
relates
producing
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69720026T
Other languages
German (de)
Other versions
DE69720026D1 (en
Inventor
G Fotheringham
P Taylor
L Ton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PCBU Services Inc
Original Assignee
PCBU Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PCBU Services Inc filed Critical PCBU Services Inc
Publication of DE69720026D1 publication Critical patent/DE69720026D1/en
Application granted granted Critical
Publication of DE69720026T2 publication Critical patent/DE69720026T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine

Abstract

The present invention relates to materials and methods for production of natural and unnatural D-amino acids. In particular, the present invention relates to a fermentation method for the production of D-amino acids using recombinant host cells. Specifically, the invention relates to a method for producing a D-amino acid in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene and a L-aminodeaminase gene; (b) culturing the cell in a cell culture medium; and (c) isolating the D-amino acid from the cell culture medium. The invention also relates to a method for producing D-phenylalanine in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene, a L-aminodeaminase gene and means for increasing production of phenylpyruvate; (b) culturing the cell in a cell culture medium; and (c) isolating the D-phenylalanine from the cell culture medium. The invention also relates to the preparation of recombinant cells for use in the production of enantiomerically pure D-amino acids.
DE69720026T 1996-09-30 1997-09-25 METHOD FOR PRODUCING D-AMINO ACIDS BY DIRECT FERMENTATION Expired - Lifetime DE69720026T2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/723,896 US5728555A (en) 1996-09-30 1996-09-30 Preparation of d-amino acids by direct fermentative means
PCT/US1997/017133 WO1998014602A2 (en) 1996-09-30 1997-09-25 Preparation of d-amino acids by direct fermentative means

Publications (2)

Publication Number Publication Date
DE69720026D1 DE69720026D1 (en) 2003-04-24
DE69720026T2 true DE69720026T2 (en) 2004-01-15

Family

ID=24908159

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69720026T Expired - Lifetime DE69720026T2 (en) 1996-09-30 1997-09-25 METHOD FOR PRODUCING D-AMINO ACIDS BY DIRECT FERMENTATION

Country Status (9)

Country Link
US (1) US5728555A (en)
EP (1) EP0937156B1 (en)
JP (1) JP4548864B2 (en)
KR (1) KR20000048776A (en)
AT (1) ATE234933T1 (en)
AU (1) AU728884B2 (en)
CA (1) CA2267205A1 (en)
DE (1) DE69720026T2 (en)
WO (1) WO1998014602A2 (en)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358714B1 (en) * 1995-04-03 2002-03-19 The Nutrasweet Company Materials and methods for the production of D-phenylalanine
JPH10286098A (en) * 1997-02-14 1998-10-27 Daicel Chem Ind Ltd Production of d-amino acid, and production of amine
WO2000023609A1 (en) * 1998-10-19 2000-04-27 Nsc Technologies Llc Transaminase biotransformation process employing glutamic acid
US6365380B2 (en) * 2000-02-23 2002-04-02 Pcbu Services, Inc. Method for stereoselectively inverting a chiral center of a chemical compound using an enzyme and a metal catalyst
AU2001285833A1 (en) * 2000-07-24 2002-02-05 Degussa A.G. Process for the fermentative preparation of l-glutamic acid using coryneform bacteria
US8048665B2 (en) * 2000-07-24 2011-11-01 Evonik Degussa Gmbh Nucleotide sequences encoding alanine racemase from coryneform
DE10046934A1 (en) 2000-09-21 2002-04-18 Consortium Elektrochem Ind Process for the fermentative production of non-proteinogenic L-amino acids
CN1280424C (en) * 2000-11-01 2006-10-18 协和发酵工业株式会社 Process for producing D-serine
US7297800B2 (en) * 2001-12-27 2007-11-20 Ajinomoto Co., Inc. Process of producing glutamate derivatives
US7572607B2 (en) * 2002-04-23 2009-08-11 Cargill, Incorporated Polypeptides and biosynthetic pathways for the production of monatin and its precursors
US8372989B2 (en) * 2002-04-23 2013-02-12 Cargill, Incorporated Polypeptides and biosynthetic pathways for the production of monatin and its precursors
WO2004018672A1 (en) 2002-08-26 2004-03-04 Ajinomoto Co., Inc. NOVEL ALDOLASES AND PROCESS FOR PRODUCING SUBSTITUTED α-KETO ACID
DE10251184A1 (en) * 2002-11-04 2004-05-13 Degussa Ag Recombinant microorganism for D-amino acid production, useful as intermediates for pharmaceuticals, has genes involved in D-amino acid degradation inactivated, for improved yield
AU2003289264A1 (en) 2002-12-09 2004-06-30 Ajinomoto Co., Inc. Mutant d-aminotransferase and process for producing optically active glutamic acid derivative using the same
CN1832690A (en) * 2003-08-01 2006-09-13 嘉吉有限公司 Monatin tabletop sweetener compositions and methods of making the same
BRPI0413931A (en) * 2003-08-25 2006-10-24 Cargill Inc beverage compositions comprising monatin and methods for their manufacture
CN1890377B (en) * 2003-10-21 2013-06-05 嘉吉有限公司 Production of monatin and monatin precursors
US7180370B2 (en) * 2004-09-01 2007-02-20 Micron Technology, Inc. CMOS amplifiers with frequency compensating capacitors
US8153405B2 (en) * 2005-04-20 2012-04-10 Cargill, Incorporated Products and methods for in vivo secretion of monatin
US8158389B2 (en) * 2005-04-20 2012-04-17 Cargill, Incorporated Products and methods for in vivo secretion of monatin
EP3168299A3 (en) 2005-04-26 2017-07-05 Cargill, Incorporated Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors
BRPI0708673A2 (en) 2006-03-07 2011-06-07 Cargill Inc A method for making aldolase activity polypeptides and polynucleotides encoding these polypeptides
US20090198072A1 (en) * 2006-05-24 2009-08-06 Cargill, Incorporated Methods and systems for increasing production of equilibrium reactions
BRPI0711972A2 (en) * 2006-05-24 2012-01-10 Cargill Inc methods and systems for increasing the production of equilibrium reactions
US8133717B2 (en) 2006-12-22 2012-03-13 Richmond Chemical Corporation Stereoinversion of amino acids in a single reactor
US7588923B2 (en) * 2007-03-02 2009-09-15 Richmond Chemical Corporation Method to increase the yield and improve purification of products from transaminase reactions
KR20120098235A (en) * 2011-02-28 2012-09-05 이화여자대학교 산학협력단 Microorganism producing unnatural amino acids and the method of producing unnatural amino acids using the microorganism
CN104744278A (en) * 2015-03-09 2015-07-01 黄冈威尔曼生物科技有限责任公司 Novel preparation technology of L-valine
CN108384818A (en) * 2018-05-18 2018-08-10 南京大学 A kind of method that enzymatic conversion method prepares D-His

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0785718B2 (en) * 1986-03-07 1995-09-20 ダイセル化学工業株式会社 Method for producing D-amino acid
US4753883A (en) * 1986-05-07 1988-06-28 Biotechnica International, Inc. Enzyme deregulation
JPS63112978A (en) * 1986-10-31 1988-05-18 Daicel Chem Ind Ltd Escherichia coli
JPH01285193A (en) * 1988-05-12 1989-11-16 Daicel Chem Ind Ltd Production of d-aspartic acid
US5120837A (en) * 1989-09-20 1992-06-09 The Nutrasweet Company Dna encoding phe a feedback inhibition resistant enzyme analogues
WO1991005870A1 (en) * 1989-10-17 1991-05-02 The Rockefeller University Enzymatic production of d-amino acids
US5354672A (en) * 1992-11-24 1994-10-11 Ian Fotheringham Materials and methods for hypersecretion of amino acids
JP3151075B2 (en) * 1992-12-22 2001-04-03 協和醗酵工業株式会社 Alanine production method

Also Published As

Publication number Publication date
EP0937156A2 (en) 1999-08-25
DE69720026D1 (en) 2003-04-24
EP0937156B1 (en) 2003-03-19
AU4499597A (en) 1998-04-24
CA2267205A1 (en) 1998-04-09
JP4548864B2 (en) 2010-09-22
JP2001505048A (en) 2001-04-17
WO1998014602A3 (en) 1998-07-09
WO1998014602A2 (en) 1998-04-09
AU728884B2 (en) 2001-01-18
ATE234933T1 (en) 2003-04-15
KR20000048776A (en) 2000-07-25
US5728555A (en) 1998-03-17

Similar Documents

Publication Publication Date Title
DE69720026T2 (en) METHOD FOR PRODUCING D-AMINO ACIDS BY DIRECT FERMENTATION
DE69939947D1 (en) METHOD OF PREPARING POLYPEPTIDES IN MUTATED ASPERGILLUS CELLS
HUP0003383A2 (en) 13c, 15n, 2h labeled proteins for nmr structure determinations and their preparation
DE69636903D1 (en) PREPARATION OF 1,3-PROPANGLIUM FROM GLYCEROL WITH RECOMBINANT DIOL DEHYDRATASE EXPRESSING BACTERIA
EP1942189A3 (en) Method for producing secreted proteins
ATE414162T1 (en) METHOD AND MATERIALS FOR PRODUCING GLUCOSAMINE
DE60030988D1 (en) Process for producing L-amino acids by increasing cellular NADPH
DE69723855D1 (en) METHOD FOR PRODUCING POLYPEPTIDES IN SURFACTIN MUTANTS FROM BACILLUS CELLS
KR840002031A (en) DNA, recombinant DNA, hosts and polypeptides having them
AU4200897A (en) Novel host cells and methods of producing proteins
DE69938722D1 (en) METHOD FOR THE PRODUCTION OF HETEROLOGIC POLYPEPTIDES IN TRICHOTHECENE LACK OF FILAMENT SOLVENTS MUSHROOMS
DE69430975D1 (en) Process for the production of proteins
EP2298911A3 (en) Methods for producing polypeptides in respiratory-deficient cells
DE60019280D1 (en) METHOD FOR THE FERMENTATIVE MANUFACTURE OF L-AMINO ACIDS USING AN AMPLIFIED GND GENE
WO2002040686A3 (en) Expression system for recombinant proteins
ATE451469T1 (en) IN VIVO PROCESS FOR PRODUCING PROTEINS CONTAINING NON-CONVENTIONAL AMINO ACIDS
DE60036615D1 (en) METHOD FOR THE FERMENTATIVE MANUFACTURE OF L-AMINO ACIDS BY REINFORCEMENT OF THE TKT GENE
NZ515762A (en) Method of producing a heparin-binding protein by expressing it in a mammalian cell that can be cultured in anaerobic conditions
ATE312195T1 (en) METHOD FOR PRODUCING FOREIGN PROTEINS
HUP9802388A2 (en) A dna molecule for expression of bile salt-stimulated lipase (bssl)
JPS557015A (en) Proline acylase, its preparation and preparation of l-proline
WO2000052133A3 (en) Hansenula polymorpha mutants and process for the preparation of recombinant proteins using the same
ATE84568T1 (en) FERMENTATION PROCESS FOR PRODUCTION OF HEPATITIS VACCINE.
TNSN01128A1 (en) Process for the preparation of a neutrophil inhibitory factor
AU2099800A (en) Production of proteins