DE696938C - Process for the production of permanent bacterial preparations - Google Patents

Process for the production of permanent bacterial preparations

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Publication number
DE696938C
DE696938C DE1936W0097979 DEW0097979D DE696938C DE 696938 C DE696938 C DE 696938C DE 1936W0097979 DE1936W0097979 DE 1936W0097979 DE W0097979 D DEW0097979 D DE W0097979D DE 696938 C DE696938 C DE 696938C
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DE
Germany
Prior art keywords
production
permanent
preparations
bacterial preparations
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE1936W0097979
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German (de)
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Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Individual filed Critical Individual
Priority to DE1936W0097979 priority Critical patent/DE696938C/en
Application granted granted Critical
Publication of DE696938C publication Critical patent/DE696938C/en
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Fodder In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

Verfahren zur Herstellung von Bakteriendauerpräparaten Im Patent 695 o46 ist ein Verfahren zur Herstellung von Bakteriendauerpräparaten, vornehmlich des Termobacterium mobile Lindner und des Bacterium acidophilum, beschrieben worden. Die vorliegende Erfindung bedient sich des Verfahrens nach dem Patent 695 o46 zur Herstellung von Präparaten der Bakterien-. gruppe der sogenannten wilden Müchsäurebildner, insbesondere des Bacterium cucumeris und verwandter Arten, welche die gemeinsame Eigenschaft haben, neben Milchsäure Acetyltrimethylaminoäthylalkohol zu bilden. Von dieser Bakteriengruppe ist bekannt, daß sie zur ErzielungeinerMüchsäuregärungverwendetwerden. Für die Großtechnik brauchbare Reinzucht-Verfahren für diese Arten von Bakterien sind bisher nicht zur Anwendung gelangt, da eine technische Verwertung von Präparaten dieser Mikroorganismen außerhalb des Bedürfnisbereiches lag.Process for the production of permanent bacterial preparations In the patent 695 046 a process for the production of permanent bacterial preparations, primarily the Termobacterium mobile Lindner and the Bacterium acidophilum, has been described. The present invention makes use of the method according to the patent 695 o46 for the production of preparations of the bacterial. group of so-called wild muschic acid formers, especially the Bacterium cucumeris and related species, which have the common property of forming acetyltrimethylaminoethyl alcohol in addition to lactic acid. This group of bacteria is known to be used to achieve malic acid fermentation. Pure breeding methods for these types of bacteria that can be used in large-scale technology have not yet been used, since the technical utilization of preparations of these microorganisms was outside of the area of need.

AlsNährlösung organischer bzw, anorganischer Zusammensetzung haben sich z. B. die nachstehenden Zusammenstellungen bewährt.As a nutrient solution of organic or inorganic composition z. B. the following compilations are proven.

i. 4o kg Rübenzuckermelasse werden in _401 Wasser gelöst, zentrifugiert und sterilisiert. Diese Mischung wird mit 2o1 sterilisierter Peptonlösung . (i: i in Wasser) und 2o 1 sterilisierter Hefeautolysatlösung (i:i in Wasser) in iooo 1 Wasser aufgelöst und, in üblicher Weise beimpft, bei 30' der Gärung über. lassen.i. 40 kg of beet sugar molasses are dissolved in 40 liters of water, centrifuged and sterilized. This mixture is sterilized with 2o1 peptone solution. (i: i in water) and 2o 1 sterilized yeast autolysate solution (i: i in water) dissolved in 100o 1 water and, inoculated in the usual way, over fermentation at 30 '. permit.

2. ioo kg Roggenschrot werden i Stunde aufgekocht, mit 3oo 1 Wasser abfiltriert, sterilisiert und zentrifugiert. Die klare Flüssigkeit wird unter Zusatz von Pepton und Autolysat mit 7oo 1 Wasser verdünnt, beimpft und bei 30' der Gärung überlassen.2. 100 kg of rye meal are boiled for one hour, filtered off with 300 liters of water, sterilized and centrifuged. The clear liquid is diluted with 700 liters of water with the addition of peptone and autolysate, inoculated and left to ferment for 30 minutes.

Die Weiterverarbeitung der durch Ausschleudern gewonnenen Bakterienkonzentrate erfolgt entsprechend dem Verfahren nach . Patent 695046. The further processing of the bacterial concentrates obtained by centrifuging is carried out according to the method according to. Patent 695046.

Die so gewonnenen Präparate können überall verwendet werden, wo es sich um die Erzielung einer Milchsäuregärung handelt, so beispielsweise bei derZubereitung vonNahrungsmitteln, wie Gurken, Kohl oder ähnlichen Erzeugnissen. Ein weiteres Anwendungsgebiet ist ferner gegeben bei der Daueraufbewahrung von Futter- und Nahrungsmitteln nach Art der Gä,rfutterbereitung.The preparations obtained in this way can be used anywhere there is lactic acid fermentation is involved, for example in preparation of foods such as cucumber, cabbage or similar products. Another area of application is also given for the permanent storage of feed and food Type of forage preparation.

Claims (1)

PATENTANSPRUCH: Anwendung des Verfahrens zur Herstellung von Bakteriendauerpräparaten nach Patent 695 o46 auf die Herstellung von Dauerpräparaten der Müchsäurebüdner, insbesondere des Bacterium cucumeris.PATENT CLAIM: Application of the process for the production of permanent bacteria preparations according to patent 695 046 on the production of permanent preparations of the Müchsäurebüdner, in particular of the Bacterium cucumeris.
DE1936W0097979 1936-01-24 1936-01-24 Process for the production of permanent bacterial preparations Expired DE696938C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE1936W0097979 DE696938C (en) 1936-01-24 1936-01-24 Process for the production of permanent bacterial preparations

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE1936W0097979 DE696938C (en) 1936-01-24 1936-01-24 Process for the production of permanent bacterial preparations

Publications (1)

Publication Number Publication Date
DE696938C true DE696938C (en) 1940-10-02

Family

ID=7614874

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1936W0097979 Expired DE696938C (en) 1936-01-24 1936-01-24 Process for the production of permanent bacterial preparations

Country Status (1)

Country Link
DE (1) DE696938C (en)

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