DE635572C - Process for the production of butanol by fermentation - Google Patents

Process for the production of butanol by fermentation

Info

Publication number
DE635572C
DE635572C DEI49002D DEI0049002D DE635572C DE 635572 C DE635572 C DE 635572C DE I49002 D DEI49002 D DE I49002D DE I0049002 D DEI0049002 D DE I0049002D DE 635572 C DE635572 C DE 635572C
Authority
DE
Germany
Prior art keywords
fermentation
butanol
waste liquor
production
preculture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEI49002D
Other languages
German (de)
Inventor
Dr Curt Schumann
Dr Karl Vierling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IG Farbenindustrie AG
Original Assignee
IG Farbenindustrie AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IG Farbenindustrie AG filed Critical IG Farbenindustrie AG
Priority to DEI49002D priority Critical patent/DE635572C/en
Application granted granted Critical
Publication of DE635572C publication Critical patent/DE635572C/en
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

Verfahren zur Herstellung von Butanol durch Gärung Es ist bekannt, Butanol durch Vergärung zuckerhaltiger Lösungen, z. B. Melassemaische, zu gewinnen, und es ist ferner bekannt, daß Butanol neben anderen Stoffen aus den bei der Natronzellstoffherstellung anfallenden und praktisch zuckerfreien Schwarzlaugen durch Vergärung nach schwachem Ansäuern entsteht. Bei Verwendung von Sulfitablauge, die bekanntlich geringe Mengen von vergärbarem Zucker enthält, als Gärflüssigkeit konnte jedoch eine befriedigende Vergärung bis jetzt nicht erzielt werden.Process for the production of butanol by fermentation It is known Butanol by fermentation of sugary solutions, e.g. B. molasses mash to win, and it is also known that butanol, among other substances, is obtained from the sodium pulp production accruing and practically sugar-free black liquor through fermentation after weak Acidification occurs. When using sulphite waste liquor, which is known to be small amounts Containing fermentable sugar, however, as a fermentation liquid could be a satisfactory one Fermentation has not yet been achieved.

Es wurde nun gefunden, daß Sulfitablauge mit gutem Erfolg auf Butanol vergoren werden kann, wenn man die Ablauge mit einer größeren Menge Erdalkalihydroxyd, als zur Neutralisation des Säuregehaltes der Ablauge erforderlich ist, behandelt und die nach Abtrennung des entstandenen Niederschlages erhaltene Flüssigkeit nach Einstellung des für die Vergärung notwendigen pH-Wertes und Zugabe von Nährstoffen mit einer Vorkultur von Butylbazillen beimpft. Durch die Behandlung der Ablaugen mit überschüssigem Erdalkalihydroxyd, z. B. 2 °/o über die zur Neutralisation erforderliche Menge, wird ein großer Teil der die Entwicklung der Butylbazillen hemmenden Ligninsulfosäure zur Ausfällung gebracht, so daß sie durch anschließende Filtration u. dgl. aus der zu vergärenden Flüssigkeit entfernt werden kann. Gleichzeitig geht die reduzierende Wirkung der Ablaugen gegenüber Kupferlösungen wesentlich zurück, jedoch beruht dieser Rückgang ganz überwiegend auf dem Verschwinden nicht vergärbarer reduzierender Stoffe aus der Lösung, während der Gehalt an vergärbaren Zuckerstoffen nur unwesentlich beeinträchtigt-- wird. Als Impfmaterial kann eine Vorkultur von Butylbazillen in einer Maische aus Melasse, Rohzucker, Kartoffeln, Mais, Stärke usw. benützt werden. Die Vorkultur kann nach einem früheren Vorschlag durch unmittelbares Beimpfen der Gesamtmaische mit Sporen Butanol erzeugender Bazillen hergestellt werden.It has now been found that sulphite waste liquor with good success on butanol can be fermented if the waste liquor is mixed with a larger amount of alkaline earth hydroxide, than is necessary to neutralize the acidity of the waste liquor and the liquid obtained after separation of the precipitate formed Adjustment of the pH value necessary for fermentation and addition of nutrients inoculated with a preculture of butyl bacilli. By treating the waste liquors with excess alkaline earth metal hydroxide, e.g. B. 2% above that required for neutralization Amount, becomes a large part of the lignin sulfonic acid, which inhibits the development of butyl bacilli brought to precipitation, so that they by subsequent filtration and the like liquid to be fermented can be removed. At the same time the reducing goes Effect of the waste liquors in relation to copper solutions, but this is based Decline largely due to the disappearance of non-fermentable reducing substances from the solution, while the content of fermentable sugars is only insignificant impaired--. A preculture of butyl bacilli in a mash made from molasses, raw sugar, potatoes, corn, starch etc. can be used. The preculture can, according to an earlier proposal, by inoculating the Whole mash with spores of butanol-producing bacilli can be produced.

Beispiel Sulfitablauge wird mit Kalkmilch entsprechend einer Menge von 2o bis 25 kg Ca0 (je nach ihrem Säuregehalt) auf i cbm Ablauge innig vermischt. Der hierbei gebildete Niederschlag wird abfiltriert und ausgewaschen. Das Filtrat wird nach dem Neutralisieren mit Kohlensäure durch Behandlung mit gereinigten Feuerungsabgasen und nach Zusatz von- 0,5 kg Diammoniumphosphat je i cbm Ablauge mit einer größeren Menge einer in voller Gärung befindlichen Vorkultur eines Butylbazillus beimpft. Zur Vorkultur wird eine 4°/oige Melasselösung verwendet. Nach 3 bis ¢ Tagen ist die Gärung beendet, worauf die vergorene Maische unter Ausnutzung der Wärme der aus den Kochern kommenden heißen Ablauge destilliert wird. Man erhält je i cbm Sulfitablauge etwa 6 1 Rohbutanol, a das in bekannter Weise aufgearbeitet wird.Example Sulphite waste liquor is intimately mixed with milk of lime corresponding to an amount of 20 to 25 kg Ca0 (depending on its acidity) per 1 cbm waste liquor. The precipitate formed is filtered off and washed out. After neutralization with carbonic acid, the filtrate is inoculated with a larger amount of a preculture of a butyl bacillus in full fermentation after the addition of 0.5 kg of diammonium phosphate per 1 cbm of waste liquor. A 4% molasses solution is used for the preculture. Fermentation is over after 3 to ¢ days, whereupon the fermented mash is distilled using the warmth of the hot waste liquor coming from the digesters. About 6 l of crude butanol, which is worked up in a known manner, is obtained per 1 cubic meter of sulphite waste liquor.

Claims (1)

pA'rtNTANSPRUCH: Verfahren zur ' Herstellung von Butanol durch Gärung, dadurch gekennzeichnet, däß, man Sulfitablauge mit einer größeren Menge Erdalkalihydroxyd, als zur Neutra lisa;io des Säuregehaltes der Ablauge erfordet ist, behandelt und die nach Abtrennung des entstandenen Niederschlages erhaltene Flüssigkeit mit dem für die Vergärung notwen- mit 1 H W t 'lach Zugabe von Nährstoffen lba ill vergärt 'Be p ng eü er Vorkultur von e#
PARTNERSHIP: Process for the production of butanol by fermentation, characterized in that sulphite waste liquor is treated with a larger amount of alkaline earth metal hydroxide than is required to neutralize the acid content of the waste liquor, and the liquid obtained after separation of the precipitate formed is treated with it which is necessary for fermentation with 1 HW t 'laugh adding nutrients lba ill fermented 'Be p ng a preculture of e #
DEI49002D 1934-02-14 1934-02-14 Process for the production of butanol by fermentation Expired DE635572C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEI49002D DE635572C (en) 1934-02-14 1934-02-14 Process for the production of butanol by fermentation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEI49002D DE635572C (en) 1934-02-14 1934-02-14 Process for the production of butanol by fermentation

Publications (1)

Publication Number Publication Date
DE635572C true DE635572C (en) 1936-09-21

Family

ID=7192450

Family Applications (1)

Application Number Title Priority Date Filing Date
DEI49002D Expired DE635572C (en) 1934-02-14 1934-02-14 Process for the production of butanol by fermentation

Country Status (1)

Country Link
DE (1) DE635572C (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE761641C (en) * 1941-04-13 1952-11-17 Hirschberg Riesengeb Process for the purification of technical hydrolysates of cellulose-containing raw materials for the production of yeast
DE920724C (en) * 1951-07-14 1954-11-29 Tornescher Hefe Ges M B H Process for the fermentation of pentose-containing sugar solutions resulting from the saccharification of wood or woody plant parts

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE761641C (en) * 1941-04-13 1952-11-17 Hirschberg Riesengeb Process for the purification of technical hydrolysates of cellulose-containing raw materials for the production of yeast
DE920724C (en) * 1951-07-14 1954-11-29 Tornescher Hefe Ges M B H Process for the fermentation of pentose-containing sugar solutions resulting from the saccharification of wood or woody plant parts

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