DE69636564D1 - Verfahren zur freisetzung von magnetischen teilchen, die an zelloberflächen gebunden sind - Google Patents
Verfahren zur freisetzung von magnetischen teilchen, die an zelloberflächen gebunden sindInfo
- Publication number
- DE69636564D1 DE69636564D1 DE69636564T DE69636564T DE69636564D1 DE 69636564 D1 DE69636564 D1 DE 69636564D1 DE 69636564 T DE69636564 T DE 69636564T DE 69636564 T DE69636564 T DE 69636564T DE 69636564 D1 DE69636564 D1 DE 69636564D1
- Authority
- DE
- Germany
- Prior art keywords
- magnetic particles
- cell surfaces
- releasing magnetic
- tied
- coating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/002—High gradient magnetic separation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Soft Magnetic Materials (AREA)
- Hard Magnetic Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US41863995A | 1995-04-06 | 1995-04-06 | |
US418639 | 1995-04-06 | ||
PCT/US1996/003267 WO1996031776A1 (en) | 1995-04-06 | 1996-03-11 | Multiparameter cell separation using releasable colloidal magnetic particles |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69636564D1 true DE69636564D1 (de) | 2006-11-02 |
DE69636564T2 DE69636564T2 (de) | 2007-06-06 |
Family
ID=23658956
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69636564T Expired - Lifetime DE69636564T2 (de) | 1995-04-06 | 1996-03-11 | Verfahren zur freisetzung von magnetischen teilchen, die an zelloberflächen gebunden sind |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0819250B1 (de) |
AT (1) | ATE340254T1 (de) |
AU (1) | AU5360596A (de) |
DE (1) | DE69636564T2 (de) |
WO (1) | WO1996031776A1 (de) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5770029A (en) * | 1996-07-30 | 1998-06-23 | Soane Biosciences | Integrated electrophoretic microdevices |
US6074827A (en) | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
US6241894B1 (en) * | 1997-10-10 | 2001-06-05 | Systemix | High gradient magnetic device and method for cell separation or purification |
AU2002255255A1 (en) * | 2001-05-07 | 2002-11-18 | Gamida Volcano Ltd. | Magnetic beads and uses thereof |
US20120156779A1 (en) * | 2009-08-27 | 2012-06-21 | Ge Healthcare Bio-Sciences Ab | Method for cell expansion |
EP2700713B1 (de) | 2012-08-21 | 2016-07-13 | Miltenyi Biotec GmbH | Untersuchungs- und Anreicherungssystem für die Proteinexpression in eukaryotischen Zellen unter Verwendung einer trizistronischen Expressionskassette |
US20190178879A1 (en) * | 2012-08-23 | 2019-06-13 | Stemcell Technologies Canada Inc. | Compositions and methods for rapid and reversible biomolecular labeling |
EP3037170A1 (de) * | 2014-12-27 | 2016-06-29 | Miltenyi Biotec GmbH | Multisort-Zelltrennverfahren |
EP3037820A1 (de) | 2014-12-27 | 2016-06-29 | Miltenyi Biotec GmbH | Zellerkennungsverfahren und Reagenzien mit lösbarem Etikettierungsteil |
US10871490B2 (en) * | 2016-02-29 | 2020-12-22 | Multenyi Biotec, gmbH | Assay for detection of chimeric antigen receptor T cells |
EP3336546B1 (de) | 2016-12-13 | 2020-07-01 | Miltenyi Biotec B.V. & Co. KG | Umkehrbare zellmarkierung mit konjugaten mit zwei lösbaren bindungsstellen |
US20220252580A1 (en) | 2019-04-23 | 2022-08-11 | Miltenyi Biotec B.V. & Co. KG | Reversible cell detection with conjugates having a linker for increased fluorescent brightness and an enzymmatically releasable fluorescent moiety |
EP3976763A1 (de) | 2019-05-28 | 2022-04-06 | Miltenyi Biotec B.V. & Co. KG | Verfahren zur erzeugung von genetisch modifizierten t-zellen |
WO2021047804A1 (en) | 2019-09-11 | 2021-03-18 | Miltenyi Biotec B.V. & Co. KG | In vitro method for transduction of t cells in the presence of malignant cells |
US20230013963A1 (en) | 2019-12-20 | 2023-01-19 | Miltenyi Biotec B.V. & Co. KG | Reversible cell detection via mhc with conjugates having an enzymatically cleavable detection moiety |
KR20220129567A (ko) | 2019-12-31 | 2022-09-23 | 엘릭서젠 쎄라퓨틱스, 인크. | 핵산 및 단백질의 세포 및 조직으로의 온도-기반 일시적 전달 |
KR20220128367A (ko) * | 2019-12-31 | 2022-09-20 | 엘릭서젠 쎄라퓨틱스, 인크. | Zscan4 핵산 및 단백질의 세포 및 조직으로의 온도-기반 일시적 전달 |
WO2022043023A1 (en) | 2020-08-27 | 2022-03-03 | Miltenyi Biotec B.V. & Co. KG | Releasable reagents for cell separation |
WO2023143179A1 (zh) * | 2022-01-28 | 2023-08-03 | 南京大学 | 一种对抗原样本的处理方法和应用 |
WO2023209175A1 (en) | 2022-04-28 | 2023-11-02 | Bio-Recell Ltd. | Method for separating target cells |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4452773A (en) * | 1982-04-05 | 1984-06-05 | Canadian Patents And Development Limited | Magnetic iron-dextran microspheres |
US4714680B1 (en) * | 1984-02-06 | 1995-06-27 | Univ Johns Hopkins | Human stem cells |
JPH0825888B2 (ja) * | 1986-01-30 | 1996-03-13 | フレッド ハッチンソン キャンサ− リサ−チ センタ− | 免疫選択法 |
CA1335181C (en) * | 1988-10-11 | 1995-04-11 | R. Alan Hardwick | System for selective cell separation from cell concentrate |
US5385707A (en) * | 1988-12-28 | 1995-01-31 | Stefan Miltenyi | Metal matrices for use in high gradient magnetic separation of biological materials and method for coating the same |
US5081030A (en) * | 1989-04-25 | 1992-01-14 | The Johns Hopkins University | Release of cells from affinity matrices |
GB8929297D0 (en) * | 1989-12-29 | 1990-02-28 | Dynal As | Method of separation |
GB9108047D0 (en) * | 1991-04-16 | 1991-06-05 | Mellors Alan | The use of pasteurella haemolytica clycoproteinase for human bone marrow haematopoietic primitive stem-cell isolation |
AU678179B2 (en) * | 1992-07-28 | 1997-05-22 | Steven Kessler | Methods for positive immunoselection of stem cells |
-
1996
- 1996-03-11 AT AT96910404T patent/ATE340254T1/de not_active IP Right Cessation
- 1996-03-11 WO PCT/US1996/003267 patent/WO1996031776A1/en active IP Right Grant
- 1996-03-11 DE DE69636564T patent/DE69636564T2/de not_active Expired - Lifetime
- 1996-03-11 AU AU53605/96A patent/AU5360596A/en not_active Abandoned
- 1996-03-11 EP EP96910404A patent/EP0819250B1/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
AU5360596A (en) | 1996-10-23 |
EP0819250A4 (de) | 1998-05-06 |
EP0819250B1 (de) | 2006-09-20 |
DE69636564T2 (de) | 2007-06-06 |
EP0819250A1 (de) | 1998-01-21 |
WO1996031776A1 (en) | 1996-10-10 |
ATE340254T1 (de) | 2006-10-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE69636564D1 (de) | Verfahren zur freisetzung von magnetischen teilchen, die an zelloberflächen gebunden sind | |
DE69403460D1 (de) | Immobilisierung und abrennung von zellen und anderen teilchen | |
EP1002066A4 (de) | Zusammensetzung und verfahren zur regulierung der adhäsion von zellen und biomolekülen an hydrophobe oberflächen | |
PL316209A1 (en) | Method of and apparatus for detecting specific target cells in specialised or mixed cell populations and solutions containing moxed populations of cells | |
PL308109A1 (en) | Improved method of detecting specific target cells among specialised or mixed populations of cells or in suspensions containing mixed populations of cells | |
ES8802089A1 (es) | Metodo para determinar la presencia de uno o una pluralidad de analitos. | |
EP0811694A3 (de) | Reagenzzubereitung enthaltend magnetische Partikel in Form einer Tablette | |
DE69626418D1 (de) | Verfahren zur bulk-anreicherung von einer zellpopulation oder zellsubpopulation | |
CA2383791A1 (en) | Methods of modification of selected cells in a magnetic cell separation column | |
GR3014996T3 (en) | Test method and reagent kit therefor. | |
DE69012440D1 (de) | Verfahren zur Bestimmung von Nukleinsäuren. | |
CA2199835A1 (en) | Method for preparing nucleic acids for analysis and kits useful therefor | |
DE69633740D1 (de) | Verfahren zur auswahl einer population oder subpopulation einer probe unter verwendung von partikel-und schwerkraft -sedimentation | |
DE59908914D1 (de) | Verfahren zur herstellung von agglomeraten mit kern-schale-struktur | |
ES8606498A1 (es) | Procedimiento de ligacion de sustancias biologicamente importantes | |
KR880012752A (ko) | 활성화 인 프로테인 c의 분리 방법 | |
EP0997476A3 (de) | Antikörper gegen ein Antigen auf Langerhans Zellen in Säugetieren und deren Verwendungen | |
AU6659390A (en) | Methods for utilizing cell lines which lack human class ii histocompatibility antigens | |
WO1996000394B1 (en) | Process for the preparation of magnetic particles used in biological analyses | |
EP0347256A3 (de) | Verfahren zum Nachweis einer Substanz in einer Probe |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |