WO1996000394B1 - Process for the preparation of magnetic particles used in biological analyses - Google Patents
Process for the preparation of magnetic particles used in biological analysesInfo
- Publication number
- WO1996000394B1 WO1996000394B1 PCT/IB1995/000502 IB9500502W WO9600394B1 WO 1996000394 B1 WO1996000394 B1 WO 1996000394B1 IB 9500502 W IB9500502 W IB 9500502W WO 9600394 B1 WO9600394 B1 WO 9600394B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- glutaraldehyde
- solid surface
- excess
- preparation
- magnetic particles
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 10
- 239000006249 magnetic particle Substances 0.000 title abstract 2
- 238000002360 preparation method Methods 0.000 title abstract 2
- 238000004450 types of analysis Methods 0.000 title 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims abstract 6
- 239000002245 particle Substances 0.000 claims abstract 6
- 239000007787 solid Substances 0.000 claims 6
- 230000005298 paramagnetic Effects 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 230000001588 bifunctional Effects 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 230000001808 coupling Effects 0.000 claims 2
- 238000010168 coupling process Methods 0.000 claims 2
- 238000005859 coupling reaction Methods 0.000 claims 2
- 230000003100 immobilizing Effects 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 2
- 230000003213 activating Effects 0.000 claims 1
- 239000007825 activation reagent Substances 0.000 claims 1
- 125000003277 amino group Chemical group 0.000 claims 1
- 102000004965 antibodies Human genes 0.000 abstract 3
- 108090001123 antibodies Proteins 0.000 abstract 3
- 238000001994 activation Methods 0.000 abstract 1
- 238000004166 bioassay Methods 0.000 abstract 1
- 230000000984 immunochemical Effects 0.000 abstract 1
- 238000010791 quenching Methods 0.000 abstract 1
- 230000000171 quenching Effects 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
Abstract
An improved process for the preparation of antibody-coated magnetic particles which are used for biological separations has been invented. The new process involves the use of much lower concentration of glutaraldehyde (approximately ten times less) and surprisingly requires much less time than was used previously to activate the particles. The benefits of the novel process are: (1) it is a more efficient method of removing the excessive glutaraldehyde from the reaction system, and it eliminates the quenching step after antibody immobilization; (2) the activation process can be completed in a much shorter time than in the past, even though there is a significant reduction in glutaraldehyde concentration, which allows for the antibody immobilization step to be completed within one working day instead of two; (3) the resulting particles give 15 to 74 % improvement of the sensitivity, which is observed in some immunochemical assays.
Claims
1. A process for immobilizing proteins on a solid surface comprising a. activating said solid surface by mixing therewith approximately a 10 to 100 fold excess on a molecular basis of a bifunctional coupling reagent for about for 30 - 120 minutes, b. washing said solid surface to remove said excess activation reagent, c. immobilizing on said washed solid surface said proteins for about 4 - 8 hours, and d. washing said resulting solid surface to remove excess protein.
2. The process of claim 1 wherein the solid surface is a paramagnetic particle.
3. The process of claim 2 wherein the surface of said paramagnetic particles contain amine groups thereon.
4. The process of claim 2 wherein said bifunctional coupling reagent is glutaraldehyde.
5. The process of claim 4 wherein said excess of glutaraldehyde is in the range of 0.05 to 0.5 milligram per milligram of paramagnetic particles.
6. The process of claim 5 in which the excess of glutaraldehyde is about 0.2 milligram per milligram of paramagnetic particle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU25736/95A AU2573695A (en) | 1994-06-23 | 1995-06-21 | Process for the preparation of magnetic particles used in biological analyses |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26440194A | 1994-06-23 | 1994-06-23 | |
| US08/264,401 | 1994-06-23 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1996000394A1 WO1996000394A1 (en) | 1996-01-04 |
| WO1996000394B1 true WO1996000394B1 (en) | 1996-01-25 |
Family
ID=23005913
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/IB1995/000502 WO1996000394A1 (en) | 1994-06-23 | 1995-06-21 | Process for the preparation of magnetic particles used in biological analyses |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU2573695A (en) |
| WO (1) | WO1996000394A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2755962B1 (en) * | 1996-11-21 | 1998-12-24 | Saint Gobain Vitrage | GLAZING COMPRISING A SUBSTRATE PROVIDED WITH A STACK OF THIN FILMS FOR SUN PROTECTION AND / OR THERMAL INSULATION |
| FR2787440B1 (en) | 1998-12-21 | 2001-12-07 | Saint Gobain Vitrage | TRANSPARENT SUBSTRATE HAVING AN ANTI-REFLECTIVE COATING |
| FR3013043B1 (en) | 2013-11-08 | 2015-11-20 | Saint Gobain | SUBSTRATE COATED WITH A FUNCTIONAL LAYER STACK HAVING IMPROVED MECHANICAL PROPERTIES |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4554088A (en) * | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
| GB8400070D0 (en) * | 1984-01-04 | 1984-02-08 | Serono Diagnostics Ltd | Magnetic assay reagents |
| DE3582649D1 (en) * | 1984-11-01 | 1991-05-29 | Technicon Instr | MAGNETICALLY SENSITIVE REAGENT AND METHOD FOR THE PRODUCTION THEREOF. |
| DE585868T1 (en) * | 1992-08-31 | 1994-09-22 | Fujirebio Kk | Magnetic particle and its use in an immunoassay. |
-
1995
- 1995-06-21 AU AU25736/95A patent/AU2573695A/en not_active Abandoned
- 1995-06-21 WO PCT/IB1995/000502 patent/WO1996000394A1/en active Application Filing
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