DE69534503D1 - CONTROL OF PLANT ENGENEXPRESSION - Google Patents

CONTROL OF PLANT ENGENEXPRESSION

Info

Publication number
DE69534503D1
DE69534503D1 DE69534503T DE69534503T DE69534503D1 DE 69534503 D1 DE69534503 D1 DE 69534503D1 DE 69534503 T DE69534503 T DE 69534503T DE 69534503 T DE69534503 T DE 69534503T DE 69534503 D1 DE69534503 D1 DE 69534503D1
Authority
DE
Germany
Prior art keywords
plant
gene
promoter
specific
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69534503T
Other languages
German (de)
Other versions
DE69534503T2 (en
Inventor
John Oliver
Edwin Quisenberry
Lee Trolinder
Lee Keim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Agriculture USDA
D&PL Technology Holding Co LLC
Original Assignee
US Department of Agriculture USDA
D&PL Technology Holding Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/477,559 external-priority patent/US5723765A/en
Application filed by US Department of Agriculture USDA, D&PL Technology Holding Co LLC filed Critical US Department of Agriculture USDA
Application granted granted Critical
Publication of DE69534503D1 publication Critical patent/DE69534503D1/en
Publication of DE69534503T2 publication Critical patent/DE69534503T2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

A method for making a genetically modified plant comprising regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences, a second gene that encodes a recombinase specific for the specific excision sequences linked to a repressible promoter, and a third gene that encodes the repressor specific for the repressible promoter. Also a method for making a genetically modified hybrid plant by hybridizing a first plant regenerated from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences to a second plant regenerated from a second plant cell that has been transfected with DNA sequences comprising a second gene that encodes a recombinase specific for the specific excision sequences linked to a promoter that is active during seed germination, and growing a hybrid plant from the hybrid seed. Plant cells, plant tissues, plant seed and whole plants containing the above DNA sequences are also claimed.
DE69534503T 1994-08-01 1995-07-31 CONTROL OF PLANT ENGENEXPRESSION Expired - Fee Related DE69534503T2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US28360494A 1994-08-01 1994-08-01
US283604 1994-08-01
US477559 1995-06-07
US08/477,559 US5723765A (en) 1994-08-01 1995-06-07 Control of plant gene expression
PCT/US1995/009595 WO1996004393A2 (en) 1994-08-01 1995-07-31 Control of plant gene expression

Publications (2)

Publication Number Publication Date
DE69534503D1 true DE69534503D1 (en) 2006-02-16
DE69534503T2 DE69534503T2 (en) 2006-07-06

Family

ID=26962145

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69534503T Expired - Fee Related DE69534503T2 (en) 1994-08-01 1995-07-31 CONTROL OF PLANT ENGENEXPRESSION

Country Status (15)

Country Link
US (2) US5925808A (en)
EP (1) EP0775212B1 (en)
JP (1) JPH10503377A (en)
CN (1) CN1112445C (en)
AT (1) ATE305976T1 (en)
AU (1) AU696668B2 (en)
BR (1) BR9508471A (en)
CA (1) CA2196410C (en)
DE (1) DE69534503T2 (en)
DK (1) DK0775212T3 (en)
EG (1) EG23907A (en)
ES (1) ES2250974T3 (en)
HK (1) HK1003271A1 (en)
TR (1) TR199500933A2 (en)
WO (1) WO1996004393A2 (en)

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Also Published As

Publication number Publication date
US5977441A (en) 1999-11-02
US5925808A (en) 1999-07-20
JPH10503377A (en) 1998-03-31
WO1996004393A2 (en) 1996-02-15
CA2196410A1 (en) 1996-02-15
ATE305976T1 (en) 2005-10-15
DK0775212T3 (en) 2006-02-20
EP0775212B1 (en) 2005-10-05
HK1003271A1 (en) 1998-10-23
DE69534503T2 (en) 2006-07-06
TR199500933A2 (en) 1996-07-21
BR9508471A (en) 1997-10-28
ES2250974T3 (en) 2006-04-16
WO1996004393A3 (en) 1996-03-07
CA2196410C (en) 2005-10-11
CN1112445C (en) 2003-06-25
EP0775212A2 (en) 1997-05-28
AU3205095A (en) 1996-03-04
EG23907A (en) 2007-12-30
AU696668B2 (en) 1998-09-17
CN1161717A (en) 1997-10-08

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